##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938220_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2616479 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.517472909203555 34.0 31.0 34.0 31.0 34.0 2 32.733168506225354 34.0 31.0 34.0 31.0 34.0 3 32.78890715346846 34.0 31.0 34.0 31.0 34.0 4 36.245825401235784 37.0 35.0 37.0 35.0 37.0 5 36.21466635123003 37.0 37.0 37.0 35.0 37.0 6 36.24487374062624 37.0 37.0 37.0 35.0 37.0 7 36.20305991372375 37.0 37.0 37.0 35.0 37.0 8 36.1855558557894 37.0 37.0 37.0 35.0 37.0 9 37.87442895586015 39.0 38.0 39.0 35.0 39.0 10-11 37.84658543026717 39.0 38.0 39.0 35.0 39.0 12-13 37.75858032875479 39.0 38.0 39.0 35.0 39.0 14-15 39.04474027882509 40.0 38.0 41.0 36.0 41.0 16-17 38.92481995842505 40.0 38.0 41.0 35.0 41.0 18-19 39.16926354081191 40.0 39.0 41.0 36.0 41.0 20-21 39.14479305968059 40.0 39.0 41.0 36.0 41.0 22-23 39.04767743215214 40.0 39.0 41.0 35.5 41.0 24-25 38.94864663542111 40.0 38.0 41.0 35.0 41.0 26-27 38.7748900335145 40.0 38.0 41.0 35.0 41.0 28-29 38.65788622801865 40.0 38.0 41.0 34.5 41.0 30-31 38.42357362700025 40.0 38.0 41.0 34.0 41.0 32-33 38.2190287023133 40.0 38.0 41.0 34.0 41.0 34-35 38.071453086380586 40.0 38.0 41.0 33.0 41.0 36-37 38.08258312029258 40.0 38.0 41.0 33.5 41.0 38-39 37.940802888156185 40.0 37.5 41.0 33.0 41.0 40-41 37.7691191100712 40.0 37.0 41.0 33.0 41.0 42-43 37.531323010809565 40.0 37.0 41.0 33.0 41.0 44-45 37.25909992015988 40.0 37.0 41.0 32.0 41.0 46-47 36.87844542226404 39.5 36.0 41.0 31.0 41.0 48-49 36.48239064788977 39.0 35.5 41.0 30.0 41.0 50-51 36.09821213929101 39.0 35.0 40.5 29.5 41.0 52-53 35.564731075617274 38.5 35.0 40.0 28.0 41.0 54-55 35.23138366484119 38.0 34.5 40.0 27.5 41.0 56-57 34.870011951175606 38.0 34.0 40.0 26.0 41.0 58-59 34.416213927189936 37.5 33.5 40.0 26.0 41.0 60-61 33.914018610506716 37.0 33.0 40.0 25.0 41.0 62-63 33.418707736618565 36.0 32.5 39.0 23.0 40.5 64-65 32.89779642794763 36.0 32.0 39.0 22.0 40.0 66-67 32.3317039808078 35.0 31.0 38.5 20.5 40.0 68-69 31.732707008158673 35.0 31.0 38.0 18.5 40.0 70-71 31.09430975750235 34.5 30.0 37.0 15.0 39.0 72-73 30.492097395010624 34.0 29.5 36.5 9.5 39.0 74-75 29.877197562067188 34.0 29.0 36.0 7.0 38.5 76-77 28.563212622765175 32.5 27.0 34.5 7.0 36.5 78-79 29.24250739256841 34.0 29.0 35.0 2.0 37.0 80-81 28.91804998243823 34.0 29.0 35.0 2.0 37.0 82-83 28.355119227022268 33.5 27.5 35.0 2.0 36.0 84-85 27.762018537125655 33.0 26.5 35.0 2.0 36.0 86-87 27.107622495728037 33.0 25.5 35.0 2.0 35.0 88-89 26.346513004690657 32.0 24.5 34.5 2.0 35.0 90-91 25.787709551653194 32.0 23.5 34.0 2.0 35.0 92-93 24.938387428295812 31.0 19.0 34.0 2.0 35.0 94-95 24.084483957257063 31.0 12.0 34.0 2.0 35.0 96-97 23.00958100561862 30.5 2.0 34.0 2.0 35.0 98-99 21.54234641287012 30.0 2.0 34.0 2.0 35.0 100-101 17.19799967819348 23.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1475520279672793 1101 2 0.11706615614927074 1101 3 0.12007631501212757 1101 4 0.10422598585466147 1101 5 0.05136355142671789 1101 6 0.10898264174376493 1101 7 0.10395264445630659 1101 8 0.1008813747967352 1101 9 -0.025937535431253878 1101 10-11 0.07802413270793807 1101 12-13 0.15599209352429 1101 14-15 0.18178026688251236 1101 16-17 0.22450594471952456 1101 18-19 0.2430572243854101 1101 20-21 0.18922692089191884 1101 22-23 0.23883235486316323 1101 24-25 0.2093577018043291 1101 26-27 0.10541034717716258 1101 28-29 0.23937432722885177 1101 30-31 0.18029954624075373 1101 32-33 0.1841996897711624 1101 34-35 0.16813587813049935 1101 36-37 0.1812304800765432 1101 38-39 0.23957837011798944 1101 40-41 0.25840703895234896 1101 42-43 0.255315288408724 1101 44-45 0.18977250685923508 1101 46-47 0.12885985926548926 1101 48-49 0.10519370895021751 1101 50-51 -0.05848975609804086 1101 52-53 -0.2795411873364202 1101 54-55 -0.3395732123320414 1101 56-57 -0.15691049395586276 1101 58-59 -1.5169979146634205E-4 1101 60-61 -0.19048060053714977 1101 62-63 -0.21171882209343806 1101 64-65 -0.3109868722372724 1101 66-67 -0.34224881274550256 1101 68-69 -0.29451069577908484 1101 70-71 -0.3513340245604084 1101 72-73 -0.6307787145256718 1101 74-75 -0.6001488372527781 1101 76-77 -0.5541268804807729 1101 78-79 -0.6568028148058751 1101 80-81 -0.7079911095138911 1101 82-83 -0.726494153852915 1101 84-85 -0.660982214545303 1101 86-87 -0.6438159156213175 1101 88-89 -0.4607695139036885 1101 90-91 -0.5334544126979139 1101 92-93 -0.8626524775893749 1101 94-95 -0.8589244287649471 1101 96-97 -0.8392523440088588 1101 98-99 -0.6431017140837945 1101 100-101 -0.8682013162385012 1102 1 0.09730258766548161 1102 2 0.1076768882521506 1102 3 0.14174589034661977 1102 4 0.09220349215677714 1102 5 0.10256491938201151 1102 6 0.1038175528090477 1102 7 0.142232475929859 1102 8 0.13010907941711025 1102 9 0.23234996434839417 1102 10-11 0.1897731675590606 1102 12-13 0.19729627848030873 1102 14-15 0.2520873957629419 1102 16-17 0.21241293786211912 1102 18-19 0.29942808834790924 1102 20-21 0.1936604357966374 1102 22-23 0.18250335808914286 1102 24-25 0.14229197472550936 1102 26-27 0.11739097368657525 1102 28-29 0.06469560281190923 1102 30-31 0.14209243146296302 1102 32-33 0.07506955773526869 1102 34-35 0.18639437937329006 1102 36-37 0.14875917011831774 1102 38-39 0.10706982039739188 1102 40-41 -0.025098831268174138 1102 42-43 0.04278972738840281 1102 44-45 -0.18890519511689519 1102 46-47 -0.19072450972986843 1102 48-49 -0.1820218145218533 1102 50-51 -0.08492888561308831 1102 52-53 -0.013377147284948876 1102 54-55 -0.06191112020659517 1102 56-57 -0.08149887338717576 1102 58-59 -0.22537844367004567 1102 60-61 -0.2992151978188602 1102 62-63 0.008292151517004243 1102 64-65 0.0021918169230943363 1102 66-67 -0.21090813722943835 1102 68-69 -0.21391745975391174 1102 70-71 -0.3728559629671615 1102 72-73 -0.3891246080155426 1102 74-75 -0.2358481074415728 1102 76-77 -0.2609034154641101 1102 78-79 -0.4175340362700801 1102 80-81 -0.5703868822499771 1102 82-83 -0.5028396330779756 1102 84-85 -0.5943087169163093 1102 86-87 -0.7687509113023872 1102 88-89 -0.7902455570577445 1102 90-91 -1.047851216688958 1102 92-93 -0.9126419006154443 1102 94-95 -0.9387243917455379 1102 96-97 -0.9129396974736999 1102 98-99 -0.7132413742103658 1102 100-101 -1.2413051733083265 1103 1 0.1369573116625915 1103 2 0.13056741495270074 1103 3 0.08999455654935673 1103 4 0.020936191453145625 1103 5 0.020621968649912503 1103 6 0.06425631763194417 1103 7 0.03248503632855204 1103 8 0.0749485668453218 1103 9 0.13745933923856057 1103 10-11 0.20172488726130666 1103 12-13 0.09316423079398817 1103 14-15 0.1704750826527146 1103 16-17 0.17562684449983124 1103 18-19 0.1621720906534918 1103 20-21 0.206082499158164 1103 22-23 0.26401734978058045 1103 24-25 0.23395328160080453 1103 26-27 0.23813723237647366 1103 28-29 0.3032734480703425 1103 30-31 0.3671031510457041 1103 32-33 0.3444184045893337 1103 34-35 0.2826992864819573 1103 36-37 0.2546689990970421 1103 38-39 0.2981798595502312 1103 40-41 0.44862562625063873 1103 42-43 0.3682815102131656 1103 44-45 0.36847796143118927 1103 46-47 0.3771909883265465 1103 48-49 0.40083353085958606 1103 50-51 0.3640950172991282 1103 52-53 0.2672243618917136 1103 54-55 0.30311834985941033 1103 56-57 0.23915688390741252 1103 58-59 0.3252544510390507 1103 60-61 0.1735819279271098 1103 62-63 -0.04897229883199117 1103 64-65 0.14604958387585043 1103 66-67 0.09589484386895464 1103 68-69 0.20799661839712158 1103 70-71 0.07804254894387697 1103 72-73 -0.04519602152432611 1103 74-75 -0.2145782586404117 1103 76-77 -0.2094902757159005 1103 78-79 -0.1609917519094246 1103 80-81 -0.24054848435312337 1103 82-83 -0.2764617273681296 1103 84-85 -0.4336516064940632 1103 86-87 -0.5310293146820406 1103 88-89 -0.7029432542804912 1103 90-91 -0.9954476577874871 1103 92-93 -0.9639936224264609 1103 94-95 -1.0062242863070203 1103 96-97 -1.2697641177375765 1103 98-99 -1.2454286787097786 1103 100-101 -1.8565200394365533 1104 1 0.12162058392736697 1104 2 0.08309961923600184 1104 3 0.09750184670377848 1104 4 0.012095127278868745 1104 5 -0.025357254932949047 1104 6 0.024350200596202853 1104 7 0.026852895219612094 1104 8 0.04543400536327624 1104 9 0.1096350058117892 1104 10-11 0.030260455265796793 1104 12-13 0.14410243287665025 1104 14-15 0.20285382761655057 1104 16-17 0.054123294602540284 1104 18-19 0.09650338371979217 1104 20-21 0.12020510763189662 1104 22-23 0.0807397298225041 1104 24-25 0.1126944800729035 1104 26-27 0.09176682977587092 1104 28-29 -0.06009336907124663 1104 30-31 -0.07154157054779375 1104 32-33 -0.1305465566483761 1104 34-35 -0.14406907278354453 1104 36-37 -0.05919714655543373 1104 38-39 0.03709443190998485 1104 40-41 0.08156373629118008 1104 42-43 0.0431121736276765 1104 44-45 0.009765547040821332 1104 46-47 -0.15768460837834652 1104 48-49 -0.20866061400634806 1104 50-51 -0.05353857974882459 1104 52-53 -0.15511677131415524 1104 54-55 -0.200193493033062 1104 56-57 -0.10946018321221374 1104 58-59 0.06800048590627483 1104 60-61 -0.35156105114167246 1104 62-63 -0.3412733937148644 1104 64-65 -0.22139888299525978 1104 66-67 -0.4199075333280291 1104 68-69 -0.34787755165488576 1104 70-71 -0.37377716564187224 1104 72-73 -0.5111082217075733 1104 74-75 -0.49923776554566146 1104 76-77 -0.5898560176381586 1104 78-79 -0.5681229244842179 1104 80-81 -0.48945464740992506 1104 82-83 -0.6057963883393391 1104 84-85 -0.6548175291535792 1104 86-87 -0.768131912361742 1104 88-89 -0.7179410996580948 1104 90-91 -0.8384476452362897 1104 92-93 -0.8127684162730233 1104 94-95 -0.8052850382911565 1104 96-97 -0.8311813153896672 1104 98-99 -0.8263316119248536 1104 100-101 -1.3138041274455574 1105 1 0.0967912775651314 1105 2 0.14900520732019373 1105 3 0.05483569247751774 1105 4 0.03780760899376645 1105 5 0.08028965532592025 1105 6 0.07932276804028504 1105 7 0.05945822329258732 1105 8 0.055526989766526924 1105 9 0.13163752356746272 1105 10-11 0.043295919540717875 1105 12-13 0.09795691287382624 1105 14-15 0.09488054249443678 1105 16-17 0.03424047612801928 1105 18-19 0.08731906596490546 1105 20-21 0.14626936922748968 1105 22-23 0.1194990488519565 1105 24-25 0.0786636003094614 1105 26-27 0.02282973837191804 1105 28-29 0.07980560696301353 1105 30-31 0.06789777781624906 1105 32-33 -0.03850140960282289 1105 34-35 0.05512226488200156 1105 36-37 -0.08613477915918821 1105 38-39 0.00309061213736328 1105 40-41 -0.03760179633963645 1105 42-43 -0.03170909317206139 1105 44-45 -0.19370597526014421 1105 46-47 -0.30567530601628334 1105 48-49 -0.18147591192116153 1105 50-51 -0.2585246771883263 1105 52-53 -0.28118495464755 1105 54-55 -0.36166477218628046 1105 56-57 -0.40549256107949816 1105 58-59 -0.17621632344628324 1105 60-61 -0.287854773615976 1105 62-63 -0.3551037942419484 1105 64-65 -0.3694315432276625 1105 66-67 -0.4887952108356828 1105 68-69 -0.4729621631479297 1105 70-71 -0.5150550246867702 1105 72-73 -0.5637171577793261 1105 74-75 -0.5917448933041136 1105 76-77 -0.5811615249345543 1105 78-79 -0.7523685193922311 1105 80-81 -0.6605224189608556 1105 82-83 -0.6108664109677697 1105 84-85 -0.792475232034878 1105 86-87 -0.8314260152446131 1105 88-89 -1.0515817665103881 1105 90-91 -1.1811055638131691 1105 92-93 -1.14063118682391 1105 94-95 -0.9851873085851679 1105 96-97 -1.033434353756025 1105 98-99 -1.199622224461077 1105 100-101 -1.7124801487641221 1106 1 0.15307695378467656 1106 2 0.11831487733841328 1106 3 0.14380896137520693 1106 4 0.127619544814209 1106 5 0.09306898747228587 1106 6 0.025344939853674475 1106 7 0.08700290345890238 1106 8 0.04640287502125062 1106 9 0.08318032810979048 1106 10-11 0.06853257864606377 1106 12-13 -0.01164380761494499 1106 14-15 0.05702503475230003 1106 16-17 0.11034227544663366 1106 18-19 0.15259726910329618 1106 20-21 0.2192992286768103 1106 22-23 0.2413645160882325 1106 24-25 0.23328535235681613 1106 26-27 0.19315413134629011 1106 28-29 0.14035270433107883 1106 30-31 0.17352300599698367 1106 32-33 0.2642636650328285 1106 34-35 0.29806479707819733 1106 36-37 0.2135583556071552 1106 38-39 0.23737430199498277 1106 40-41 0.26892395628909327 1106 42-43 0.2037902051992191 1106 44-45 0.16626900488488872 1106 46-47 0.24409171172428046 1106 48-49 0.23679723360753968 1106 50-51 0.26167876918422195 1106 52-53 0.2661349274857443 1106 54-55 0.10299892185862092 1106 56-57 0.2205244544982179 1106 58-59 0.1930147053265543 1106 60-61 0.03827609441380275 1106 62-63 0.2730534917093834 1106 64-65 0.07635799238237695 1106 66-67 -0.17189789778815623 1106 68-69 0.00385207913631902 1106 70-71 -0.5224632203330373 1106 72-73 -0.4681492710919315 1106 74-75 -0.41341120526329433 1106 76-77 -0.4314634588689863 1106 78-79 -0.22611609916248554 1106 80-81 -0.24336556384934838 1106 82-83 -0.29751271223381437 1106 84-85 -0.4895972566014031 1106 86-87 -0.6365932381964363 1106 88-89 -0.7102429447275433 1106 90-91 -1.1300966372964467 1106 92-93 -1.0771316254499972 1106 94-95 -1.1244125063129644 1106 96-97 -1.3428393795285523 1106 98-99 -1.1897968270589239 1106 100-101 -1.719582084774892 1107 1 -0.0378171875816804 1107 2 0.02482340651332038 1107 3 0.034881063736570184 1107 4 0.06448469055262507 1107 5 0.00981684414986006 1107 6 0.04088461407992838 1107 7 0.0038314808409651846 1107 8 -0.009203365627818982 1107 9 -0.04351644925687026 1107 10-11 0.05451533445091883 1107 12-13 0.06930591840396261 1107 14-15 0.012280891227135271 1107 16-17 0.011427499615535908 1107 18-19 0.10372369837599393 1107 20-21 0.0484557348594592 1107 22-23 0.04429805666728015 1107 24-25 0.08826309784631547 1107 26-27 0.024843953591791035 1107 28-29 0.01795430447825197 1107 30-31 0.03774749590783699 1107 32-33 -0.03266650780334146 1107 34-35 0.1035194517531437 1107 36-37 0.0039351335745365645 1107 38-39 0.06917628847519808 1107 40-41 0.022579384005574354 1107 42-43 0.12112751074210593 1107 44-45 0.06211348424573515 1107 46-47 0.025834659456684506 1107 48-49 0.026024680148843515 1107 50-51 0.03293365701060935 1107 52-53 -4.8415150978797783E-4 1107 54-55 0.033481500265644115 1107 56-57 -0.01348862225590608 1107 58-59 -0.08588236826633278 1107 60-61 -0.2536681670011305 1107 62-63 -0.1507062722107122 1107 64-65 -0.5068462816850641 1107 66-67 -0.5157948493613702 1107 68-69 -0.34332860946760846 1107 70-71 -0.5407262797228256 1107 72-73 -0.6293090962095889 1107 74-75 -0.5312986317933728 1107 76-77 -0.49353627269887923 1107 78-79 -0.4298734602337042 1107 80-81 -0.41545647413485653 1107 82-83 -0.5399892609919341 1107 84-85 -0.7247385459926363 1107 86-87 -0.7830271664225883 1107 88-89 -0.9619024122337052 1107 90-91 -1.0922428506506563 1107 92-93 -1.2288935894785986 1107 94-95 -1.36090399027864 1107 96-97 -1.2993984999356876 1107 98-99 -1.287222962921252 1107 100-101 -1.7585570444614707 1108 1 -0.0192772002205146 1108 2 -0.034974644924005815 1108 3 -0.021470859593897273 1108 4 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-0.4313422462347063 2316 48-49 -0.29943248031961645 2316 50-51 -0.14634710001516993 2316 52-53 -0.11438045998180968 2316 54-55 -0.15741535611405055 2316 56-57 -0.13690345685627392 2316 58-59 -0.20549004353970446 2316 60-61 -0.035832174032613295 2316 62-63 0.09085731932411534 2316 64-65 0.2545006580222591 2316 66-67 0.25404488764294797 2316 68-69 0.3411941401157499 2316 70-71 0.49610841196495414 2316 72-73 0.8025526484197769 2316 74-75 1.1442960763316385 2316 76-77 0.9295780117108627 2316 78-79 0.9062467826720315 2316 80-81 1.0666621022183627 2316 82-83 1.1774449330130423 2316 84-85 1.3487424941187705 2316 86-87 1.4156802280481067 2316 88-89 1.4056024390321902 2316 90-91 1.5729929779455603 2316 92-93 1.6873010775533395 2316 94-95 2.3367260807210606 2316 96-97 2.763038091165381 2316 98-99 3.2086237827354367 2316 100-101 3.60908067441623 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 22.0 5 43.0 6 84.0 7 239.0 8 583.0 9 1125.0 10 1988.0 11 3310.0 12 4930.0 13 7443.0 14 10049.0 15 13959.0 16 18492.0 17 24891.0 18 25953.0 19 21255.0 20 20843.0 21 22357.0 22 23763.0 23 26273.0 24 29481.0 25 33259.0 26 39171.0 27 45977.0 28 55126.0 29 66182.0 30 80135.0 31 97489.0 32 120927.0 33 155703.0 34 209679.0 35 295006.0 36 415474.0 37 494669.0 38 240513.0 39 10078.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52021129600361 18.260744925436747 14.516241851362741 23.702801927196905 2 16.183521419585816 19.445148422051712 39.291277392533196 25.08005276582927 3 19.82139178183561 22.81042676310063 29.08392193496823 28.284259520095528 4 13.631344743332352 15.92320513423202 34.974203862674884 35.47124625976074 5 14.479602210620456 36.55844155844156 33.59776186144638 15.364194369491605 6 34.31860414808224 34.637128798771464 16.16969330058652 14.874573752559778 7 30.13205915277745 31.85670513694167 19.38895745006935 18.622278260211527 8 27.17568151703109 34.63203794106507 18.603321486623816 19.588959055280018 9 26.429220337713392 16.68547693293162 19.449305727277 37.43599700207798 10-11 25.960193068623905 25.933229351353482 26.00861692373606 22.09796065628656 12-13 26.55361811793582 22.97807684208072 26.890428545166227 23.57787649481723 14-15 23.283122088883573 24.585177255387872 25.018488587143256 27.1132120685853 16-17 23.02556973994549 27.735166428235907 25.863940440595577 23.37532339122302 18-19 23.01344669687775 26.244984194407827 28.055566278192945 22.686002830521478 20-21 23.438579098093275 25.302152243530333 28.61727535363364 22.641993304742748 22-23 23.37054874126641 25.056363915017087 28.50510170347249 23.067985640244007 24-25 23.294400118330373 25.45542000689114 28.37998802554728 22.870191849231205 26-27 22.746649046954914 25.62376015836334 28.48789348815438 23.141697306527366 28-29 22.880728351967985 26.09125230404949 28.30503178608865 22.722987557893873 30-31 22.930431588711677 25.605038673323644 28.288703995523623 23.175825742441052 32-33 22.717648412236443 25.607008502648025 28.337510066008555 23.337833019106977 34-35 23.35915533872223 25.676542402008373 28.115859988594337 22.848442270675058 36-37 23.383064611677906 25.331420827055773 28.11130068007867 23.17421388118765 38-39 22.860204294573727 25.67563745548303 28.226491332806912 23.23766691713633 40-41 23.352265593602894 25.590541475638496 28.082368613610857 22.974824317147753 42-43 23.045732346458898 25.569095957301773 27.908657994346065 23.476513701893264 44-45 23.017425902792628 25.756833086914337 28.078904527593103 23.146836482699936 46-47 23.40772474121681 26.135439423101914 27.43287154172253 23.023964293958745 48-49 23.24050084292992 26.04592295953057 27.44339795350146 23.270178244038053 50-51 23.304969030630353 26.257889970304337 27.35819074899608 23.078950250069227 52-53 23.452073696170043 26.161184810891026 27.19855367300741 23.188187819931525 54-55 23.2937202299731 26.078639119430218 27.311121099749037 23.316519550847644 56-57 23.098398323287096 26.406890332869402 27.233244051862503 23.26146729198099 58-59 23.25058431088015 26.04344505260518 27.25407909011882 23.45189154639585 60-61 23.15602353700642 26.21477948341427 27.33401225849123 23.29518472108808 62-63 23.022657478776114 26.232965510624222 27.466996587702617 23.277380422897046 64-65 22.897290399922323 26.275612987670293 27.318041803423448 23.509054808983937 66-67 22.86604087491077 26.254146117246606 27.22043153065427 23.65938147718835 68-69 22.99597985012416 26.046465217608223 27.40057770419641 23.5569772280712 70-71 23.038231664395127 26.102586537908373 27.1330848302861 23.7260969674104 72-73 23.032441094154755 26.183443967424175 26.942304329587984 23.84181060883308 74-75 22.88266644495985 26.594439937042907 26.83332903298558 23.689564585011663 76-77 23.12651354280352 26.276536574457225 26.964408927489476 23.632540955249784 78-79 23.005326596416324 26.33988261111985 27.063418169835217 23.5913726226286 80-81 23.00368971813581 26.38773421950784 26.93929987474147 23.669276187614884 82-83 23.105977987115406 26.508575742242375 26.763296973988233 23.622149296653987 84-85 22.962797028288374 26.52145018212428 26.763428810403628 23.75232397918372 86-87 23.011929331988377 26.410795904951474 26.800883359702993 23.776391403357156 88-89 22.96460815523366 26.5850639973776 26.49878484782166 23.951542999567074 90-91 22.98069661319577 26.43184100219265 26.656591932554075 23.930870452057498 92-93 22.91819738046288 26.238607093133403 26.751015527458804 24.092179998944907 94-95 22.7844474748218 26.14308881246052 26.883159203433355 24.189304509284327 96-97 22.83966969064173 26.00790568225212 26.715411111149333 24.43701351595681 98-99 22.595324871903173 26.20852500874365 26.552403595311493 24.64374652404169 100-101 23.27715589511447 25.47402162178071 26.37544198093077 24.873380502174047 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1078.0 1 738.5 2 544.5 3 706.5 4 573.5 5 542.0 6 1096.0 7 2371.0 8 3925.0 9 4479.5 10 3651.0 11 2831.0 12 2688.0 13 2712.5 14 2916.0 15 3078.5 16 3119.0 17 3210.5 18 3264.0 19 3487.0 20 3850.5 21 4434.0 22 5300.5 23 6456.5 24 7879.5 25 9703.5 26 12257.5 27 15118.0 28 17314.0 29 19642.0 30 22856.0 31 26525.5 32 31991.5 33 35416.0 34 37243.0 35 40099.0 36 44490.5 37 50191.5 38 54744.0 39 58187.5 40 61308.0 41 67158.0 42 72737.0 43 76316.5 44 80666.0 45 86194.5 46 91753.5 47 98410.0 48 102480.5 49 102950.5 50 102628.5 51 99575.5 52 99008.5 53 103064.5 54 107533.0 55 103869.5 56 94389.5 57 86774.5 58 80562.0 59 72062.0 60 60125.5 61 50126.0 62 41634.0 63 31955.5 64 23466.0 65 17749.0 66 13171.0 67 10232.0 68 7853.0 69 5822.5 70 4494.0 71 3071.0 72 2155.0 73 1648.0 74 1215.5 75 849.0 76 539.0 77 304.5 78 171.0 79 116.0 80 71.0 81 40.0 82 23.0 83 12.5 84 6.0 85 5.0 86 4.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0093255095875029 2 0.014981966222545643 3 0.009554825397031659 4 7.643860317625328E-5 5 7.261667301744061E-4 6 0.0015669913651131923 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.408246349387861E-4 14-15 0.0 16-17 0.002522473904816358 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0028855572699035613 26-27 0.017943962095625456 28-29 0.006172417206482453 30-31 0.0029046669206976242 32-33 0.0 34-35 0.00898153587320976 36-37 6.497281269981528E-4 38-39 0.002331377396875725 40-41 0.001738978222259762 42-43 0.002694460761962928 44-45 1.910965079406332E-5 46-47 1.1465790476437991E-4 48-49 3.8219301588126637E-4 50-51 0.003955697714371107 52-53 0.007510092762066885 54-55 0.006611939174745908 56-57 0.01559347504795567 58-59 0.022052537016349072 60-61 0.0033824081905492074 62-63 0.019090541143269257 64-65 0.01991225612741398 66-67 0.012478601968523347 68-69 0.029830164889532843 70-71 0.01584190050827849 72-73 0.0046436451429573864 74-75 0.01572724260351411 76-77 0.019644721016297093 78-79 0.02459412057195949 80-81 0.011083597460556725 82-83 0.033422779238816744 84-85 0.020848629016323082 86-87 0.029944822794297225 88-89 0.02107794482585184 90-91 0.026543304952953952 92-93 0.022816923048111604 94-95 0.014389567047929678 96-97 0.014752650413016882 98-99 0.01123647466690923 100-101 0.010491198285940763 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2616479.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.97388969790343 #Duplication Level Percentage of deduplicated Percentage of total 1 73.84702005858571 35.42728794809453 2 14.070405244480732 13.500241384070414 3 4.854406575998208 6.986542968771453 4 2.2491158446803956 4.315953418020168 5 1.2364490488194249 2.9658635142570353 6 0.7397959759588869 2.1294534329762675 7 0.5181759519311462 1.7401241173435646 8 0.36205170389871266 1.389522280621987 9 0.2789553461731217 1.2044315707154827 >10 1.6006033449898336 14.382755172166403 >50 0.15289909960016904 5.101086164435826 >100 0.08294163912686228 7.08746008911281 >500 0.005504793746883299 1.7810520486858161 >1k 0.0014360331513589594 1.0540873422758288 >5k 1.5955923903988436E-4 0.5385448238322205 >10k+ 7.977961951994218E-5 0.3955937246201287 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10336 0.395034701214877 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8735 0.3338455993722862 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5336 0.20393819327424373 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.821930158812664E-5 0.0 10-11 0.0 0.0 0.0 3.821930158812664E-5 0.0 12-13 0.0 0.0 0.0 7.643860317625328E-5 7.643860317625328E-5 14-15 0.0 0.0 0.0 7.643860317625328E-5 9.55482539703166E-5 16-17 0.0 0.0 0.0 1.1465790476437992E-4 1.1465790476437992E-4 18-19 0.0 0.0 0.0 1.1465790476437992E-4 1.1465790476437992E-4 20-21 0.0 0.0 0.0 1.7198685714656987E-4 1.1465790476437992E-4 22-23 0.0 0.0 0.0 2.4842546032282313E-4 1.1465790476437992E-4 24-25 0.0 0.0 0.0 5.541798730278362E-4 1.5287720635250655E-4 26-27 0.0 0.0 0.0 0.001146579047643799 1.7198685714656987E-4 28-29 0.0 0.0 0.0 0.003363298539755144 2.2931580952875985E-4 30-31 0.0 0.0 0.0 0.011542229079614245 2.484254603228232E-4 32-33 0.0 0.0 0.0 0.02940975257206345 2.675351111168865E-4 34-35 0.0 0.0 0.0 0.056220592636134284 2.675351111168865E-4 36-37 0.0 0.0 0.0 0.09935107447833519 2.675351111168865E-4 38-39 0.0 0.0 0.0 0.16065483422569032 2.675351111168865E-4 40-41 0.0 0.0 0.0 0.22570408552868187 2.675351111168865E-4 42-43 0.0 0.0 0.0 0.2886321655935324 2.675351111168865E-4 44-45 0.0 0.0 0.0 0.3677461198809545 2.675351111168865E-4 46-47 0.0 0.0 0.0 0.46281663258141953 2.675351111168865E-4 48-49 0.0 0.0 0.0 0.5593776980438214 2.675351111168865E-4 50-51 0.0 0.0 0.0 0.6638310492841716 2.675351111168865E-4 52-53 0.0 0.0 0.0 0.7636216457307703 2.675351111168865E-4 54-55 0.0 0.0 0.0 0.8748207037014246 2.675351111168865E-4 56-57 0.0 0.0 0.0 0.9996258330374521 2.675351111168865E-4 58-59 0.0 0.0 0.0 1.1298963225005818 2.675351111168865E-4 60-61 0.0 0.0 0.0 1.2739830894878192 2.8664476191094977E-4 62-63 0.0 0.0 0.0 1.4121649743796914 3.057544127050131E-4 64-65 0.0 0.0 0.0 1.5518374120335001 3.057544127050131E-4 66-67 0.0 0.0 0.0 1.7119762856877507 3.057544127050131E-4 68-69 0.0 0.0 0.0 1.8930784462630887 3.057544127050131E-4 70-71 0.0 0.0 0.0 2.0808116556639664 3.4397371429313974E-4 72-73 0.0 0.0 0.0 2.2695003476045477 4.77741269851583E-4 74-75 0.0 0.0 0.0 2.4606541844975633 5.35070222233773E-4 76-77 0.0 1.910965079406332E-5 0.0 2.6711852072957587 5.35070222233773E-4 78-79 0.0 3.821930158812664E-5 0.0 2.902660407364248 5.35070222233773E-4 80-81 0.0 3.821930158812664E-5 0.0 3.1461937970837908 5.35070222233773E-4 82-83 0.0 3.821930158812664E-5 0.0 3.39398863893041 5.35070222233773E-4 84-85 0.0 3.821930158812664E-5 0.0 3.6432931431897604 5.35070222233773E-4 86-87 0.0 3.821930158812664E-5 0.0 3.9036812449096665 5.923991746159629E-4 88-89 0.0 3.821930158812664E-5 0.0 4.180408098058498 6.497281269981529E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6530 0.0 43.723995 1 GTATCAA 9555 0.0 36.345043 1 ATCAACG 11760 0.0 29.158972 3 TCAACGC 11805 0.0 29.00426 4 TATCAAC 11870 0.0 28.97376 2 CAACGCA 12010 0.0 28.588266 5 AACGCAG 12390 0.0 27.673136 6 CGCAGAG 14310 0.0 23.861078 8 ACGCAGA 14605 0.0 23.37912 7 GAGTACT 8950 0.0 22.737196 12-13 TAAGGTG 1170 0.0 22.729752 5 TACATGG 8550 0.0 22.556772 2 GTACATG 8915 0.0 22.221493 1 GTACTTT 9105 0.0 22.141068 14-15 GCAGAGT 15640 0.0 21.437233 9 GTAAGGT 990 0.0 21.1062 4 ACATGGG 8905 0.0 20.64046 3 AGTACTT 9145 0.0 20.175133 12-13 CAGAGTA 14915 0.0 20.09144 10-11 GGACCGA 800 0.0 19.58919 6 >>END_MODULE