FastQCFastQC Report
Thu 26 May 2016
SRR938219_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938219_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377799
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135780.9854848203547832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105910.7686897726010833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72600.5269273674897427No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36820.2672378191593984No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25330.18384394240379037No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24190.17556987630271179No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19900.14443325913286337No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13990.10153875855621901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44200.055.4745031
GTATCAA78400.042.349251
ATCAACG100900.033.016853
TATCAAC102000.032.6141972
TCAACGC102550.032.4392744
CAACGCA104900.031.576655
AACGCAG107050.030.9823536
TACCGTA804.490619E-429.6914257
GTGGTAT18700.028.3161321
GTACATG85950.027.3623661
TGGTATC19200.026.9795112
ACGCAGA123650.026.6634527
TACATGG87450.026.4653662
CGCAGAG125550.026.2221058
TACCGGA950.001223140525.0123882
ACATGGG89900.024.951183
GCAGAGT131450.024.358499
GAGTACT78100.023.84265112-13
GTACTTT82250.022.52454814-15
CATGGGG40100.022.2803634
Produced by FastQC (version 0.11.5)