FastQCFastQC Report
Thu 26 May 2016
SRR938219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938219_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377799
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129720.9415016268701023No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109890.7975764244276561No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72600.5269273674897427No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36840.2673829782138033No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26110.189505145525581No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23770.17252153616020913No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18960.13761078357583365No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13890.10081296328419458No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41700.056.122781
GTATCAA75750.043.328791
TATCAAC101050.032.433442
TCAACGC101550.032.126224
ATCAACG101800.032.096373
CAACGCA104550.031.2487265
AACGCAG107900.030.3721336
TGGTATC18400.027.4030042
GTGGTAT18850.027.2535151
ACGCAGA123300.026.5401767
CGCAGAG125200.026.061548
GTACATG84300.025.0533331
GTGTACG1151.2696371E-424.8178141
GCAGAGT132750.024.400439
TACATGG85950.023.7422312
GAGTACT80750.023.08579312-13
ACATGGG85700.022.3651183
GTACTTT83500.022.18327914-15
CAGAGTA131050.020.9296810-11
TAAGGTG5000.020.9243665
Produced by FastQC (version 0.11.5)