##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938219_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1377799 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47737079211119 33.0 31.0 34.0 31.0 34.0 2 32.69582718524255 34.0 31.0 34.0 31.0 34.0 3 32.749569421954874 34.0 31.0 34.0 31.0 34.0 4 36.22395646970276 37.0 35.0 37.0 35.0 37.0 5 36.154284478360054 37.0 35.0 37.0 35.0 37.0 6 36.21361461287169 37.0 37.0 37.0 35.0 37.0 7 36.18472505786403 37.0 37.0 37.0 35.0 37.0 8 36.185547383907235 37.0 37.0 37.0 35.0 37.0 9 37.8577288849825 39.0 38.0 39.0 35.0 39.0 10-11 37.835444792745534 39.0 38.0 39.0 35.0 39.0 12-13 37.74893362529657 39.0 38.0 39.0 35.0 39.0 14-15 38.954919041166384 40.0 38.0 41.0 35.5 41.0 16-17 38.87636077541064 40.0 38.0 41.0 35.0 41.0 18-19 39.14358480445987 40.0 38.5 41.0 36.0 41.0 20-21 39.130814073750955 40.0 39.0 41.0 36.0 41.0 22-23 39.01043766180698 40.0 38.5 41.0 35.0 41.0 24-25 38.9005090728038 40.0 38.0 41.0 35.0 41.0 26-27 38.73048608686753 40.0 38.0 41.0 35.0 41.0 28-29 38.58993728403054 40.0 38.0 41.0 34.5 41.0 30-31 38.31726979044113 40.0 38.0 41.0 34.0 41.0 32-33 38.052011940783814 40.0 37.0 41.0 34.0 41.0 34-35 37.92967842188882 40.0 37.0 41.0 33.0 41.0 36-37 37.87303409278131 40.0 37.0 41.0 33.0 41.0 38-39 37.683596809113666 40.0 37.0 41.0 33.0 41.0 40-41 37.44994262588375 40.0 36.0 41.0 33.0 41.0 42-43 37.16941476949831 40.0 36.0 41.0 32.5 41.0 44-45 36.832313349044384 39.5 35.0 41.0 31.0 41.0 46-47 36.38513418865887 39.0 35.0 41.0 30.5 41.0 48-49 35.92612420244172 39.0 35.0 40.5 29.5 41.0 50-51 35.543291147692806 39.0 35.0 40.0 28.0 41.0 52-53 35.02123568096653 38.0 34.5 40.0 26.5 41.0 54-55 34.64604670202257 38.0 34.0 40.0 26.0 41.0 56-57 34.28425844408365 38.0 34.0 40.0 25.0 41.0 58-59 33.789616627679365 37.0 33.0 40.0 23.0 41.0 60-61 33.32802643927017 36.5 33.0 39.5 22.0 41.0 62-63 32.776572272152904 36.0 32.0 39.0 20.5 40.0 64-65 32.21918654317502 35.0 31.0 39.0 18.0 40.0 66-67 31.66884502020977 35.0 31.0 38.0 14.0 40.0 68-69 31.077627070421737 35.0 30.0 38.0 8.0 40.0 70-71 30.48650419981434 34.5 29.5 37.0 7.0 39.0 72-73 29.840191130926932 34.0 29.0 36.0 2.0 39.0 74-75 29.202655830059392 34.0 28.0 36.0 2.0 38.5 76-77 27.910029329386944 32.0 26.5 34.5 2.0 36.5 78-79 28.595151760162402 33.5 28.0 35.0 2.0 37.0 80-81 28.319019682841983 34.0 27.5 35.0 2.0 37.0 82-83 27.790923422066644 33.0 26.5 35.0 2.0 36.0 84-85 27.159082710903405 33.0 25.5 35.0 2.0 36.0 86-87 26.4643997419072 32.5 24.5 35.0 2.0 35.0 88-89 25.694646679232605 32.0 22.0 34.5 2.0 35.0 90-91 25.067370494535126 32.0 19.0 34.0 2.0 35.0 92-93 24.144380276078003 31.0 11.0 34.0 2.0 35.0 94-95 23.26797050948651 31.0 2.0 34.0 2.0 35.0 96-97 22.233239754129592 30.0 2.0 34.0 2.0 35.0 98-99 20.846938849570947 29.5 2.0 34.0 2.0 35.0 100-101 17.021205197565102 22.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2627879058378255 1101 2 0.24358359456341816 1101 3 0.2200801805071535 1101 4 0.13327675236929792 1101 5 0.11726772770049365 1101 6 0.10773570017544642 1101 7 0.14745719810719038 1101 8 0.12244364714813116 1101 9 0.029512767791779027 1101 10-11 0.213380320658608 1101 12-13 0.3039479798697684 1101 14-15 0.4798358830523384 1101 16-17 0.439477944134552 1101 18-19 0.39299897654427696 1101 20-21 0.352388258255786 1101 22-23 0.27832466330871597 1101 24-25 0.35541818131183334 1101 26-27 0.43611463656143457 1101 28-29 0.5583331775260731 1101 30-31 0.6283289604479521 1101 32-33 0.6094118471619154 1101 34-35 0.6479523624831884 1101 36-37 0.6437735481983609 1101 38-39 0.6749177867855778 1101 40-41 0.7652737214589322 1101 42-43 0.7911298922371373 1101 44-45 0.7576634093967911 1101 46-47 0.8378586514698512 1101 48-49 1.0032894024064092 1101 50-51 1.1501220359283764 1101 52-53 1.2787978786004004 1101 54-55 1.2931652063489167 1101 56-57 1.3206933221351989 1101 58-59 1.4361945737875672 1101 60-61 1.413981075075057 1101 62-63 1.5669967714453534 1101 64-65 1.5727093861962373 1101 66-67 1.8816204193834913 1101 68-69 2.1196993185371014 1101 70-71 2.1491281779538554 1101 72-73 2.374727789907059 1101 74-75 2.4199327286149135 1101 76-77 2.280869940754407 1101 78-79 2.387978221523131 1101 80-81 2.5314430836573614 1101 82-83 2.567424964567998 1101 84-85 2.7040555766999717 1101 86-87 2.9213435915587773 1101 88-89 3.31764862450105 1101 90-91 3.6428004906664135 1101 92-93 3.641127797202998 1101 94-95 3.8806865723199273 1101 96-97 4.426232205697623 1101 98-99 4.820810325863906 1101 100-101 5.018224936898012 1102 1 0.1231711111450835 1102 2 0.11462746427610426 1102 3 0.20685629283755702 1102 4 0.10332551035014603 1102 5 -0.0555606092907297 1102 6 0.042096359592846966 1102 7 0.09798156462754548 1102 8 0.09737194423512108 1102 9 0.08945702657070598 1102 10-11 0.15595002836013094 1102 12-13 0.24827378543640322 1102 14-15 0.32893242634843034 1102 16-17 0.27264521767804695 1102 18-19 0.31714882939743205 1102 20-21 0.2043469947881107 1102 22-23 0.27315800079874464 1102 24-25 0.22491700912507895 1102 26-27 0.37621901106263067 1102 28-29 0.3108742664127462 1102 30-31 0.36630656419895047 1102 32-33 0.2946999681714928 1102 34-35 0.2502028986962799 1102 36-37 0.2918148116660362 1102 38-39 0.41275759624287645 1102 40-41 0.5001119846273312 1102 42-43 0.5815756349546106 1102 44-45 0.33424056173118544 1102 46-47 0.41907404702052986 1102 48-49 0.5969468121230861 1102 50-51 0.5640495474008418 1102 52-53 0.65780794194837 1102 54-55 0.8509053799323283 1102 56-57 1.002740471476777 1102 58-59 0.8889872463733539 1102 60-61 0.800524308814083 1102 62-63 1.0048661973232456 1102 64-65 0.9043715220701429 1102 66-67 1.2078752203611494 1102 68-69 1.5108982535929698 1102 70-71 1.511442997387217 1102 72-73 1.624847502596598 1102 74-75 1.808323403495205 1102 76-77 1.7336560665524914 1102 78-79 1.6288601050027793 1102 80-81 1.8647163528113921 1102 82-83 1.8191554987858751 1102 84-85 2.2217502968715586 1102 86-87 2.301247447304984 1102 88-89 2.265739456504239 1102 90-91 2.6083272888507736 1102 92-93 2.8377200383243064 1102 94-95 2.891147092072522 1102 96-97 3.019232130877196 1102 98-99 3.3212205912270356 1102 100-101 3.023217018402402 1103 1 0.2771083599355393 1103 2 0.19264227758154107 1103 3 0.19389260663008656 1103 4 0.08036784279483555 1103 5 -0.020609660991389944 1103 6 0.056172584533207726 1103 7 0.13064831808355137 1103 8 0.10865141409060897 1103 9 0.1491523708256608 1103 10-11 0.2162387475695695 1103 12-13 0.19300155305732147 1103 14-15 0.3520624147702236 1103 16-17 0.3992463395246233 1103 18-19 0.3101707776672171 1103 20-21 0.2679107538231662 1103 22-23 0.3057241289312529 1103 24-25 0.33283703966486655 1103 26-27 0.27521099688451756 1103 28-29 0.42878450485073927 1103 30-31 0.43310511293022813 1103 32-33 0.4283222219649119 1103 34-35 0.27274643015459077 1103 36-37 0.3626088146483184 1103 38-39 0.3911319564073281 1103 40-41 0.6148717070775334 1103 42-43 0.6200185964333542 1103 44-45 0.7251928986599907 1103 46-47 0.8163016306857713 1103 48-49 0.8389249298886767 1103 50-51 0.8434986489110372 1103 52-53 1.0311370112836187 1103 54-55 0.8960993572551459 1103 56-57 0.9767700188452011 1103 58-59 1.0588003411240479 1103 60-61 1.2257087518875025 1103 62-63 1.3415685922688354 1103 64-65 1.3753769640134195 1103 66-67 1.4892079021455054 1103 68-69 1.6464160369177137 1103 70-71 1.7038310690098015 1103 72-73 1.9958467812388143 1103 74-75 1.9493139466015172 1103 76-77 1.9289962336031081 1103 78-79 2.0299994981188725 1103 80-81 2.0398779979599624 1103 82-83 2.246965633894238 1103 84-85 2.3710691577677423 1103 86-87 2.5703444042065335 1103 88-89 2.8487619519242138 1103 90-91 3.1003744292991975 1103 92-93 3.04103822125737 1103 94-95 3.401151135952709 1103 96-97 3.4042790191334014 1103 98-99 3.3967276327985054 1103 100-101 2.992229135578075 1104 1 0.19253268569421067 1104 2 0.21071853881889524 1104 3 0.19128730919195647 1104 4 0.1475748674486681 1104 5 9.724943293676347E-4 1104 6 0.1266764361920778 1104 7 0.18196624478141388 1104 8 0.2700833627566013 1104 9 0.29073902912964655 1104 10-11 0.29278142137668084 1104 12-13 0.2667057392235108 1104 14-15 0.4156027245111389 1104 16-17 0.39906139557068343 1104 18-19 0.35140523835455895 1104 20-21 0.4251694019632737 1104 22-23 0.3106130821484854 1104 24-25 0.46190379639119783 1104 26-27 0.445118203265892 1104 28-29 0.4984199240302374 1104 30-31 0.44343785122648427 1104 32-33 0.4018269903025029 1104 34-35 0.5580988690024711 1104 36-37 0.5811964861235239 1104 38-39 0.6377667805497467 1104 40-41 0.7158671880122185 1104 42-43 0.7813058063527833 1104 44-45 0.7949971797104709 1104 46-47 0.8118710168326686 1104 48-49 1.0869749773308115 1104 50-51 1.1755132514423607 1104 52-53 1.317663442186948 1104 54-55 1.3006682770216358 1104 56-57 1.2397040340588532 1104 58-59 1.3737518536439524 1104 60-61 1.4105079670818625 1104 62-63 1.5550019561581294 1104 64-65 1.7672973255386424 1104 66-67 1.8127874521688305 1104 68-69 1.7595776136051313 1104 70-71 1.857334550780763 1104 72-73 2.0110845548352465 1104 74-75 2.1187065263284452 1104 76-77 2.2489653560038434 1104 78-79 2.255003920691685 1104 80-81 2.3322904712235015 1104 82-83 2.280199258313271 1104 84-85 2.506850184126275 1104 86-87 2.7785189269707224 1104 88-89 2.85284680099387 1104 90-91 3.2297628276845565 1104 92-93 3.5193032861485776 1104 94-95 3.746336964421211 1104 96-97 3.93196432493421 1104 98-99 4.253773997443652 1104 100-101 3.9244994432283242 1105 1 0.07943167446673272 1105 2 0.09204321639650459 1105 3 0.11896826731788224 1105 4 0.10365794648256355 1105 5 0.002440277998552176 1105 6 -0.04360674580070878 1105 7 0.08361009490301541 1105 8 -0.016687546965130196 1105 9 0.13877948724036315 1105 10-11 -0.03776419312678314 1105 12-13 0.09195387126320753 1105 14-15 0.2716636121879574 1105 16-17 0.20816390724930045 1105 18-19 0.1730258720360922 1105 20-21 0.15949577036457896 1105 22-23 0.24707990269023838 1105 24-25 0.2693281570942432 1105 26-27 0.2746240907343065 1105 28-29 0.1827492775633388 1105 30-31 0.2484225707311154 1105 32-33 0.35042048631988365 1105 34-35 0.4300924751881112 1105 36-37 0.5099149183548377 1105 38-39 0.41199281472894 1105 40-41 0.5234250347972349 1105 42-43 0.48383376239361553 1105 44-45 0.5304052359616165 1105 46-47 0.5151424396591224 1105 48-49 0.675479347451116 1105 50-51 0.6857550696467101 1105 52-53 0.9098412141071108 1105 54-55 0.8616753591060018 1105 56-57 0.9015561900562119 1105 58-59 1.0695182045625273 1105 60-61 0.8740738703433166 1105 62-63 0.9305137936137626 1105 64-65 1.1700349730069703 1105 66-67 1.2985511896890038 1105 68-69 1.5193052006160883 1105 70-71 1.5802070248241975 1105 72-73 1.7961882966134617 1105 74-75 1.6757140950456169 1105 76-77 1.5643152416113395 1105 78-79 1.6681943169031577 1105 80-81 1.7807899074278524 1105 82-83 1.76889490216114 1105 84-85 1.975882257959288 1105 86-87 2.167984565850876 1105 88-89 2.2450680544563397 1105 90-91 2.403842196143316 1105 92-93 2.577914659162758 1105 94-95 2.586884178504725 1105 96-97 2.712184841688682 1105 98-99 2.5612234219890944 1105 100-101 2.5440207407355686 1106 1 0.1445432176479784 1106 2 0.1439727823853474 1106 3 0.027045895187065128 1106 4 0.08418792326701663 1106 5 -0.00545980633188492 1106 6 0.018248370337332176 1106 7 0.031670659291883396 1106 8 0.1503174284211255 1106 9 0.2123350406026674 1106 10-11 0.10594436131351159 1106 12-13 0.1206574744429858 1106 14-15 0.10391896344233231 1106 16-17 0.06438653960915985 1106 18-19 -0.023710518028934757 1106 20-21 -0.1090168994888785 1106 22-23 0.07892511630725352 1106 24-25 0.07070228267312473 1106 26-27 -0.1396930575150961 1106 28-29 -0.034389815023594394 1106 30-31 0.2120727518386829 1106 32-33 0.271524096518057 1106 34-35 0.20188706860901817 1106 36-37 0.3141558950526573 1106 38-39 0.20042539808064674 1106 40-41 0.3407939799210382 1106 42-43 0.1708929878037324 1106 44-45 0.17362442163004488 1106 46-47 0.31512220693342385 1106 48-49 0.31803973583171796 1106 50-51 0.38882035500490275 1106 52-53 0.30262887795654336 1106 54-55 0.2900712493952824 1106 56-57 0.42325236141039824 1106 58-59 0.638492334089257 1106 60-61 0.5530197179190566 1106 62-63 0.4899280973283524 1106 64-65 0.7620713941064707 1106 66-67 0.8817707482098349 1106 68-69 1.0485656149866998 1106 70-71 0.8152918322952836 1106 72-73 1.1357285257422305 1106 74-75 1.325578826389016 1106 76-77 1.3922279590583102 1106 78-79 1.4825146453641906 1106 80-81 1.3878810665491912 1106 82-83 1.7417756475150163 1106 84-85 1.745043033009761 1106 86-87 1.894620702485927 1106 88-89 2.0716025888130396 1106 90-91 2.4109960273661954 1106 92-93 2.463830324336918 1106 94-95 2.593671188765846 1106 96-97 2.602252602128825 1106 98-99 2.6263969244658227 1106 100-101 2.7406883176007355 1107 1 0.1703407653758262 1107 2 0.12110431922809539 1107 3 0.22999658326423145 1107 4 0.07981293903100095 1107 5 -0.028856294281624173 1107 6 0.058479692351305346 1107 7 0.043371644828503975 1107 8 0.04441528462950117 1107 9 0.14921167800041957 1107 10-11 0.1760957174544373 1107 12-13 0.16386147185933453 1107 14-15 0.24036703990777397 1107 16-17 0.2830521337768772 1107 18-19 0.23301096890642725 1107 20-21 0.1900471861618982 1107 22-23 0.190075431751346 1107 24-25 0.2998795728915624 1107 26-27 0.21910993276113544 1107 28-29 0.24236179619224885 1107 30-31 0.2670514828026498 1107 32-33 0.40407175308590837 1107 34-35 0.36302839173058743 1107 36-37 0.4760102983846437 1107 38-39 0.42466047493758907 1107 40-41 0.5156008917271961 1107 42-43 0.4782450887721623 1107 44-45 0.5997047888677329 1107 46-47 0.6839203830272282 1107 48-49 0.7798012550517086 1107 50-51 0.8016269027316483 1107 52-53 0.7928516462978692 1107 54-55 1.0557886228913986 1107 56-57 1.1079645358088186 1107 58-59 1.1391903357100688 1107 60-61 1.118112618480417 1107 62-63 1.1112142407076462 1107 64-65 1.2147443619786529 1107 66-67 1.5239610257545841 1107 68-69 1.7864437997219085 1107 70-71 1.7705945061952875 1107 72-73 1.9251003681484882 1107 74-75 1.6302595495910701 1107 76-77 1.4547772386307116 1107 78-79 1.652918609004498 1107 80-81 1.5907750042981945 1107 82-83 1.9771661391209037 1107 84-85 2.0708897095241134 1107 86-87 2.2752870993829184 1107 88-89 2.4168266237558917 1107 90-91 2.4932609740866845 1107 92-93 2.700120322352028 1107 94-95 2.602532464644817 1107 96-97 2.8288018312564915 1107 98-99 3.006081842257352 1107 100-101 2.9363456289620977 1108 1 0.17648845339110153 1108 2 0.20036203280335485 1108 3 0.0714817442403799 1108 4 0.013750435281693285 1108 5 -0.1135253612892484 1108 6 0.025821606844743883 1108 7 0.027754529401590844 1108 8 0.017576712459892008 1108 9 0.07024567525034087 1108 10-11 0.13385499015348756 1108 12-13 0.1262351863614768 1108 14-15 0.22153934102392725 1108 16-17 0.09936165072492287 1108 18-19 0.10804405103125703 1108 20-21 0.21565686766778924 1108 22-23 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scores pass #Quality Count 2 3.0 3 4.0 4 11.0 5 39.0 6 75.0 7 199.0 8 381.0 9 728.0 10 1233.0 11 1977.0 12 3140.0 13 4913.0 14 6184.0 15 8500.0 16 11909.0 17 17358.0 18 17492.0 19 12784.0 20 11872.0 21 12634.0 22 14022.0 23 15134.0 24 17188.0 25 19454.0 26 22533.0 27 25768.0 28 30577.0 29 37190.0 30 44962.0 31 53690.0 32 65101.0 33 82022.0 34 110187.0 35 156783.0 36 199154.0 37 242106.0 38 125561.0 39 4931.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.28135018242864 18.851370918399564 14.76957088806132 22.097708011110473 2 16.00668690449747 20.423223273795784 39.611125870887285 23.95896395081946 3 19.224680730608853 25.289910034802993 29.682418136751455 25.802991097836696 4 12.964968333161558 17.18370540477376 35.8795513368588 33.97177492520587 5 13.661113238629301 37.746548878862505 33.112450922757525 15.479886959750665 6 31.690907019093494 35.884479521323506 16.987310921259198 15.437302538323806 7 28.758331222478752 33.754270397931776 19.671446996259977 17.815951383329498 8 27.29030474010322 34.33546451327735 18.919550557883348 19.454680188736077 9 27.423158240062595 16.623252012811736 19.670503462406344 36.283086284719325 10-11 25.600178255318813 26.889517266306623 25.75368395535198 21.756620523022587 12-13 26.60623937163549 24.448123420034417 26.95498399984323 21.99065320848687 14-15 23.33366477984089 25.42950749710226 25.991962543157605 25.244865179899246 16-17 21.90359406560754 28.96137244982759 26.55641352621101 22.57861995835387 18-19 21.824046903793658 26.590235585887346 30.09067360333401 21.495043906984982 20-21 22.769504114896293 25.57557379559718 30.362411353179965 21.292510736326562 22-23 22.456723408316698 25.63379371887462 30.487057394988877 21.422425477819807 24-25 22.019582817259096 25.942134121358883 30.465031124130356 21.573251937251666 26-27 21.6677215603329 26.274546238457262 30.39008322530351 21.667648975906324 28-29 21.628118470110664 26.29683284717147 30.586609512708314 21.48843917000956 30-31 21.6945287375009 26.151601213239378 30.42232575288558 21.731544296374146 32-33 21.461911352817065 26.31889702344101 30.29469465430008 21.924496969441844 34-35 21.947763623555186 26.319038733952194 30.158245087709684 21.57495255478294 36-37 21.973030703540235 26.04644107334287 30.0985129072739 21.88201531584299 38-39 21.661241508862865 26.246392049279716 30.259678090770286 21.832688351087132 40-41 21.785548501269737 26.258964997991143 30.224850370141315 21.730636130597805 42-43 21.895351896712846 26.19385394267672 29.866790059609677 22.044004101000752 44-45 21.678142507037744 26.443558861752447 29.831402588061184 22.046896043148628 46-47 21.91230360887183 26.691121128698743 29.377906356442413 22.018668905987013 48-49 21.967681788127297 26.813453921798462 29.032718125067593 22.18614616500665 50-51 22.042620149963817 26.98858832093796 28.80670547735918 22.162086051739042 52-53 22.23862116317402 27.00143489725279 28.660820627682266 22.09912331189092 54-55 21.93531131899501 26.920690173240075 28.729662309233785 22.414336198531135 56-57 21.958827085808597 27.26174137156436 28.545128861321572 22.234302681305472 58-59 22.10199020321542 26.86298944911413 28.58254360759443 22.45247674007602 60-61 22.033511419299913 27.073941844928033 28.747371713871182 22.145175021900872 62-63 21.984556528201864 27.146412502839674 28.698525692063935 22.170505276894524 64-65 21.843570796611118 26.981874714671733 28.652111084417974 22.522443404299175 66-67 21.88730790460289 27.199732036288328 28.481995008704104 22.430965050404687 68-69 21.8482768731864 27.03877815567875 28.604732520144637 22.50821245099021 70-71 21.852461788693418 27.117308112431495 28.421199318623398 22.60903078025169 72-73 21.923234783605874 27.17817590888653 28.171209360439864 22.727379947067732 74-75 21.792801417333006 27.48873384289 28.024842520570854 22.693622219206137 76-77 21.761918828508367 27.173121768850173 28.386107117221016 22.678852285420444 78-79 21.798752938563606 27.20875831670657 28.28326192717515 22.709226817554665 80-81 21.739406552849747 27.58536722559012 28.1525405583912 22.522685663168936 82-83 21.949115544447416 27.40725310529309 28.07242290704135 22.571208443218147 84-85 21.759040333418376 27.329041970170465 27.97448844238905 22.93742925402211 86-87 21.92863587106814 27.25427163544066 28.04657735686637 22.770515136624834 88-89 21.78245883470666 27.324050895667657 27.847603315142482 23.0458869544832 90-91 21.807713593598315 27.2746935701908 28.07102094882101 22.84657188738988 92-93 21.945653901621355 27.044910034047053 27.978899679851704 23.030536384479884 94-95 21.63445644225657 26.991774916125195 28.06936432966383 23.304404311954404 96-97 21.86785203765578 26.858139064803837 28.000470316189656 23.273538581350728 98-99 21.638715081082218 26.95451223291641 27.794148493357884 23.612624192643484 100-101 22.00110466040402 26.474108342363433 27.923267472251034 23.60151952498151 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3651.0 1 2400.5 2 1668.5 3 2100.0 4 1574.0 5 1339.5 6 2524.0 7 5232.0 8 7749.0 9 8189.0 10 6031.5 11 3664.0 12 3038.0 13 2998.0 14 3000.5 15 2944.5 16 2902.5 17 2863.0 18 2937.5 19 3051.5 20 3206.0 21 3505.5 22 3875.0 23 4403.5 24 5053.0 25 5950.5 26 6902.5 27 7882.5 28 9127.5 29 10670.5 30 12672.5 31 14980.0 32 17793.0 33 20217.5 34 21943.5 35 24004.5 36 27022.0 37 29935.0 38 31942.5 39 33232.5 40 34720.5 41 37452.0 42 39713.0 43 42309.0 44 45642.5 45 48809.5 46 50000.5 47 50732.0 48 52753.0 49 52408.0 50 51291.5 51 49595.0 52 47673.5 53 47571.5 54 47252.0 55 45238.0 56 41819.0 57 38124.5 58 35130.5 59 31434.0 60 26617.0 61 22059.0 62 17953.5 63 14024.0 64 10796.5 65 8440.0 66 6456.0 67 5254.0 68 4020.5 69 2716.5 70 1887.5 71 1368.5 72 1131.5 73 932.5 74 701.5 75 546.5 76 356.0 77 203.5 78 129.5 79 78.0 80 51.0 81 32.5 82 11.0 83 4.5 84 2.0 85 2.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013136894423642347 3 0.0031934991969075318 4 0.0012338519624415462 5 0.11990137893843733 6 0.0 7 0.0 8 1.451590544048878E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.2660787241099753E-4 24-25 6.89505508423217E-4 26-27 0.006749896029827282 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011431275534384914 36-37 7.25795272024439E-4 38-39 0.008165196810274939 40-41 0.010850639316765363 42-43 0.006278129103011397 44-45 0.003592686596520973 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.628976360122195E-5 68-69 9.435338536317706E-4 70-71 0.0 72-73 3.628976360122195E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0886929080366585E-4 82-83 0.002213675579674539 84-85 0.005116856667772295 86-87 0.003483817305717307 88-89 0.0 90-91 0.002649152742889202 92-93 0.0 94-95 1.0886929080366585E-4 96-97 1.8144881800610975E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1377799.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.37172473711141 #Duplication Level Percentage of deduplicated Percentage of total 1 76.07492538548797 40.60249977070553 2 13.23027740851623 14.122454480859037 3 4.472109066971013 7.160525223501511 4 1.9736320600318107 4.213445881614237 5 1.0927136776495885 2.916000680999526 6 0.6785516375100278 2.172928273026084 7 0.4607959492889431 1.7215432195797786 8 0.32102336395075937 1.3706856491969168 9 0.2318826207743068 1.1138377866557663 >10 1.3157564707469043 12.960099338613231 >50 0.1008733765629184 3.6700029380735666 >100 0.04487527605382229 4.271776526180548 >500 0.001223860952756912 0.3970704076133336 >1k 9.518918521442647E-4 1.0411939439994304 >5k 1.3598455030632353E-4 0.5269111970887957 >10k+ 2.7196910061264706E-4 1.7390246822926496 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12972 0.9415016268701023 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10989 0.7975764244276561 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7260 0.5269273674897427 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3684 0.2673829782138033 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2611 0.189505145525581 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2377 0.17252153616020913 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1896 0.13761078357583365 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1389 0.10081296328419458 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.0886929080366586E-4 0.0 18-19 0.0 0.0 0.0 1.8144881800610975E-4 0.0 20-21 0.0 0.0 0.0 3.628976360122195E-4 0.0 22-23 0.0 0.0 0.0 9.072440900305487E-4 0.0 24-25 0.0 0.0 0.0 0.0016330393620549877 0.0 26-27 0.0 0.0 0.0 0.0037741354145270824 0.0 28-29 0.0 0.0 0.0 0.008346645628281048 0.0 30-31 0.0 0.0 0.0 0.02384237468600282 0.0 32-33 0.0 0.0 0.0 0.04724927220879098 0.0 34-35 0.0 0.0 0.0 0.08172454762995182 0.0 36-37 0.0 0.0 0.0 0.1367398292494043 0.0 38-39 0.0 0.0 0.0 0.22060547293182822 0.0 40-41 0.0 0.0 0.0 0.3053057811770803 0.0 42-43 0.0 0.0 0.0 0.38909884533230177 0.0 44-45 0.0 0.0 0.0 0.4899480983800975 0.0 46-47 0.0 0.0 0.0 0.611010749753774 0.0 48-49 0.0 0.0 0.0 0.737081388504419 0.0 50-51 0.0 0.0 0.0 0.8696478949396829 0.0 52-53 0.0 0.0 0.0 0.9999644360316708 0.0 54-55 0.0 0.0 0.0 1.1334381865569652 0.0 56-57 0.0 0.0 0.0 1.2881777385525757 0.0 58-59 0.0 0.0 0.0 1.4492679991783999 0.0 60-61 0.0 0.0 0.0 1.6303539195484973 0.0 62-63 0.0 0.0 0.0 1.8058875060876078 0.0 64-65 0.0 0.0 0.0 1.983489609151988 0.0 66-67 0.0 0.0 0.0 2.1833736270675184 0.0 68-69 0.0 0.0 0.0 2.4026000889825 0.0 70-71 0.0 0.0 0.0 2.63333040595907 0.0 72-73 0.0 0.0 0.0 2.8585083891046517 0.0 74-75 0.0 0.0 0.0 3.0806743218713324 0.0 76-77 0.0 0.0 0.0 3.3207673978570167 0.0 78-79 0.0 0.0 0.0 3.5869528138719797 0.0 80-81 0.0 0.0 0.0 3.8750935368656823 0.0 82-83 0.0 0.0 0.0 4.167988218891145 0.0 84-85 0.0 0.0 0.0 4.452282226943117 0.0 86-87 0.0 0.0 0.0 4.739080228683575 0.0 88-89 0.0 0.0 0.0 5.049974633455243 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4170 0.0 56.12278 1 GTATCAA 7575 0.0 43.32879 1 TATCAAC 10105 0.0 32.43344 2 TCAACGC 10155 0.0 32.12622 4 ATCAACG 10180 0.0 32.09637 3 CAACGCA 10455 0.0 31.248726 5 AACGCAG 10790 0.0 30.372133 6 TGGTATC 1840 0.0 27.403004 2 GTGGTAT 1885 0.0 27.253515 1 ACGCAGA 12330 0.0 26.540176 7 CGCAGAG 12520 0.0 26.06154 8 GTACATG 8430 0.0 25.053333 1 GTGTACG 115 1.2696371E-4 24.817814 1 GCAGAGT 13275 0.0 24.40043 9 TACATGG 8595 0.0 23.742231 2 GAGTACT 8075 0.0 23.085793 12-13 ACATGGG 8570 0.0 22.365118 3 GTACTTT 8350 0.0 22.183279 14-15 CAGAGTA 13105 0.0 20.92968 10-11 TAAGGTG 500 0.0 20.924366 5 >>END_MODULE