FastQCFastQC Report
Thu 26 May 2016
SRR938218_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938218_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359733
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135850.9990932043276143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106570.7837568110798223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71130.5231174061378226No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36320.2671112637554579No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23680.17415183716214874No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23540.17312222326000767No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20200.14855857730892755No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13740.10104925011013191No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44850.054.8125151
GTATCAA80500.043.821121
TATCAAC105050.033.5712852
ATCAACG105450.033.3537943
TCAACGC106450.033.040474
CAACGCA108350.032.329485
AACGCAG111150.031.5520136
GTGGTAT19300.028.4430051
ACGCAGA126100.027.6890837
CGCAGAG127000.027.3058368
TGGTATC19850.026.3385772
GTACATG85350.025.7270071
GCAGAGT134200.025.2372139
TACATGG86600.024.9720172
GAGTACT78650.024.58238612-13
ACATGGG87550.023.5609993
GTACTTT83300.023.12715114-15
CATGGGG36450.022.1672344
GTATAAC2806.184564E-1122.162581
GTATAGG4550.022.0314391
Produced by FastQC (version 0.11.5)