##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938218_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1359733 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95656058946867 33.0 31.0 34.0 30.0 34.0 2 32.121405452393965 34.0 31.0 34.0 30.0 34.0 3 32.19030942104075 34.0 31.0 34.0 30.0 34.0 4 35.71813951709637 37.0 35.0 37.0 35.0 37.0 5 35.733080685693444 37.0 35.0 37.0 35.0 37.0 6 35.77240605324722 37.0 36.0 37.0 35.0 37.0 7 35.72538579265194 37.0 35.0 37.0 35.0 37.0 8 35.7058900534149 37.0 35.0 37.0 35.0 37.0 9 37.33923277584643 39.0 37.0 39.0 34.0 39.0 10-11 37.256638619493685 39.0 37.0 39.0 34.0 39.0 12-13 37.15141207869486 39.0 37.0 39.0 34.0 39.0 14-15 38.322056977362465 40.0 38.0 41.0 33.5 41.0 16-17 38.18903122892509 40.0 38.0 41.0 33.0 41.0 18-19 38.35411437392489 40.0 38.0 41.0 34.0 41.0 20-21 38.352961941792984 40.0 38.0 41.0 34.0 41.0 22-23 38.26020659938385 40.0 38.0 41.0 34.0 41.0 24-25 38.14495787040544 40.0 38.0 41.0 33.5 41.0 26-27 37.968960450323706 40.0 38.0 41.0 33.0 41.0 28-29 37.75546375648749 40.0 37.5 41.0 33.0 41.0 30-31 37.510162289214136 40.0 37.0 41.0 32.5 41.0 32-33 37.22548213509564 40.0 36.0 41.0 32.0 41.0 34-35 36.97745366185862 39.5 36.0 41.0 31.0 41.0 36-37 36.723757531809554 39.0 35.0 41.0 30.5 41.0 38-39 36.44780776814272 39.0 35.0 41.0 30.0 41.0 40-41 36.21114329063133 39.0 35.0 40.0 30.0 41.0 42-43 35.99890971242148 39.0 35.0 40.5 29.5 41.0 44-45 35.68427220638169 39.0 35.0 40.0 28.0 41.0 46-47 35.22904459919705 38.5 35.0 40.0 26.5 41.0 48-49 34.80293226684945 38.0 34.5 40.0 24.0 41.0 50-51 34.08235734515526 37.5 33.5 39.5 23.5 40.5 52-53 34.2097132304651 38.0 34.0 40.0 24.0 40.5 54-55 34.31186122569652 38.0 34.0 40.0 23.0 41.0 56-57 34.01448777076088 38.0 34.0 40.0 22.5 41.0 58-59 33.70950546908841 37.5 33.5 40.0 21.5 41.0 60-61 33.30410124634763 37.0 33.0 40.0 19.5 41.0 62-63 32.778854378028626 36.0 32.5 39.5 17.0 41.0 64-65 32.26673986731218 35.5 32.0 39.0 11.5 40.5 66-67 31.73858581059664 35.0 31.0 39.0 7.5 40.0 68-69 31.149188848104735 35.0 30.5 38.0 4.5 40.0 70-71 30.625238190144685 35.0 30.0 37.5 2.0 39.5 72-73 30.007197001175967 34.5 29.0 37.0 2.0 39.0 74-75 29.347855424557615 34.0 29.0 36.0 2.0 39.0 76-77 28.69709604753286 34.0 27.5 35.5 2.0 38.0 78-79 28.00099982864283 34.0 26.0 35.0 2.0 37.0 80-81 27.351937475960355 33.0 25.5 35.0 2.0 37.0 82-83 26.64706526943157 33.0 24.5 35.0 2.0 36.0 84-85 25.995934128244294 32.0 22.5 35.0 2.0 36.0 86-87 25.315190188073686 32.0 19.5 34.5 2.0 35.0 88-89 24.535973606583056 31.0 16.5 34.0 2.0 35.0 90-91 23.72328942520333 31.0 6.0 34.0 2.0 35.0 92-93 22.8281795028877 30.0 2.0 34.0 2.0 35.0 94-95 21.891900468694956 29.5 2.0 34.0 2.0 35.0 96-97 20.703147970961947 29.0 2.0 34.0 2.0 35.0 98-99 19.22931744688112 26.5 2.0 34.0 2.0 35.0 100-101 15.551415608799669 12.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32854444426609675 1101 2 0.32731113318642 1101 3 0.23593833571692358 1101 4 0.2275646549111201 1101 5 0.27687295158119696 1101 6 0.2587025103339755 1101 7 0.3496683781791816 1101 8 0.20857888666517255 1101 9 0.26260153097872774 1101 10-11 0.2638452901631254 1101 12-13 0.39835261990962323 1101 14-15 0.539934977407782 1101 16-17 0.6973352520069156 1101 18-19 0.4668549296957778 1101 20-21 0.5255308626960584 1101 22-23 0.605169646962139 1101 24-25 0.5277376694135469 1101 26-27 0.6404092578502869 1101 28-29 0.6139094644745384 1101 30-31 0.5700778837749851 1101 32-33 0.46132817151653427 1101 34-35 0.6834099812441607 1101 36-37 0.7354222911176223 1101 38-39 0.641859360708807 1101 40-41 0.6951345616199447 1101 42-43 0.7404673529419696 1101 44-45 0.6869553920133598 1101 46-47 0.6835821579985435 1101 48-49 0.743145567359754 1101 50-51 0.5213011013089002 1101 52-53 0.7872976697411858 1101 54-55 0.698085482453088 1101 56-57 0.6537997161124238 1101 58-59 0.8293916543327953 1101 60-61 0.7689598569700635 1101 62-63 0.8478688662246299 1101 64-65 0.8574760165620816 1101 66-67 0.7916897913124181 1101 68-69 0.9323393053537785 1101 70-71 1.26523718611368 1101 72-73 1.1814818017404285 1101 74-75 1.3391445441327399 1101 76-77 1.379498176329431 1101 78-79 1.3334386082943261 1101 80-81 1.5977700805785453 1101 82-83 1.8110649541848254 1101 84-85 2.112369564974827 1101 86-87 1.9894915926145416 1101 88-89 2.4569330535235316 1101 90-91 2.7874423790599003 1101 92-93 3.234202987238394 1101 94-95 3.5382993167553174 1101 96-97 3.4356302942376296 1101 98-99 3.6978193048399355 1101 100-101 3.582426189459076 1102 1 0.4161229498584582 1102 2 0.4683064339969647 1102 3 0.20763203535519636 1102 4 0.1368444394795887 1102 5 0.2522005721744094 1102 6 0.3979025483792782 1102 7 0.2832091773088976 1102 8 0.3358612333470603 1102 9 0.514771748045014 1102 10-11 0.4916106338638073 1102 12-13 0.47014797184353796 1102 14-15 0.5603724389537916 1102 16-17 0.6156736828164782 1102 18-19 0.5339415808470918 1102 20-21 0.5531615616893433 1102 22-23 0.44929982134635793 1102 24-25 0.43398869113800487 1102 26-27 0.4702685793955226 1102 28-29 0.47835672268623597 1102 30-31 0.4947181624568344 1102 32-33 0.4941663214447516 1102 34-35 0.443764099796887 1102 36-37 0.7015976383553308 1102 38-39 0.6507643395906868 1102 40-41 0.6233606101693212 1102 42-43 0.7257782989919406 1102 44-45 0.5860530049677877 1102 46-47 0.5622188283211287 1102 48-49 0.8354268291560345 1102 50-51 0.963271907482941 1102 52-53 0.790776139963306 1102 54-55 0.8478374449071211 1102 56-57 0.8711543191483244 1102 58-59 0.8184845413471606 1102 60-61 0.7922088070088478 1102 62-63 0.8798613998337927 1102 64-65 0.9664068543612743 1102 66-67 0.8039225453465626 1102 68-69 1.1149618156899166 1102 70-71 1.3052076415575797 1102 72-73 1.4969186836900086 1102 74-75 1.454775516576646 1102 76-77 1.661389800715284 1102 78-79 1.8597805226270054 1102 80-81 2.134597042809663 1102 82-83 2.4886851015509315 1102 84-85 2.584775722369436 1102 86-87 2.4624057142584554 1102 88-89 2.7718595933923353 1102 90-91 2.926390664197566 1102 92-93 3.1004342135158076 1102 94-95 3.382163174014096 1102 96-97 3.565227459862509 1102 98-99 3.840445798043792 1102 100-101 3.644714971147396 1103 1 0.44285254118288364 1103 2 0.38824038542001915 1103 3 0.3664141531492646 1103 4 0.26810706067091417 1103 5 0.26934865710038025 1103 6 0.2689873293144416 1103 7 0.32694627719621394 1103 8 0.3659847397232028 1103 9 0.34376841214560727 1103 10-11 0.42049572942226376 1103 12-13 0.4466226290491946 1103 14-15 0.6122663149565071 1103 16-17 0.6104701260113572 1103 18-19 0.5403221857717213 1103 20-21 0.7149047085916465 1103 22-23 0.6654292854826522 1103 24-25 0.6699550420656948 1103 26-27 0.765364026283315 1103 28-29 0.7877217861129466 1103 30-31 0.7613803663818146 1103 32-33 0.7176671191598274 1103 34-35 0.7476611889736304 1103 36-37 0.8194653403780663 1103 38-39 0.9419638129871686 1103 40-41 1.043669431893946 1103 42-43 0.961018338710339 1103 44-45 0.8137119622481919 1103 46-47 0.9529664491219663 1103 48-49 1.039021567583589 1103 50-51 1.0929870729948803 1103 52-53 0.9832352222004843 1103 54-55 1.0905425357797043 1103 56-57 1.111446182454543 1103 58-59 1.1211231373686275 1103 60-61 1.0892254641921895 1103 62-63 1.2413088900994325 1103 64-65 1.3482245290497232 1103 66-67 1.4338418812906006 1103 68-69 1.56448262880145 1103 70-71 1.6687102243693275 1103 72-73 1.6984739491673544 1103 74-75 1.8982738597837958 1103 76-77 1.8471123743784155 1103 78-79 2.310559828893812 1103 80-81 2.4412632632021634 1103 82-83 2.4426039465933833 1103 84-85 2.548491401096662 1103 86-87 2.6556714370552577 1103 88-89 2.8923703541782224 1103 90-91 3.1032892818633258 1103 92-93 3.3276971024716424 1103 94-95 3.491299521929438 1103 96-97 3.858300169081417 1103 98-99 4.248864760233214 1103 100-101 4.173872293948657 1104 1 0.2632720899312382 1104 2 0.395364616864903 1104 3 0.317615020518609 1104 4 0.24699855715752506 1104 5 0.23752910286235362 1104 6 0.3531194110008755 1104 7 0.24715653191733367 1104 8 0.2061630170618045 1104 9 0.33669770507489716 1104 10-11 0.35954690695849223 1104 12-13 0.3697880468138024 1104 14-15 0.4170574326552341 1104 16-17 0.5641042937759622 1104 18-19 0.4975630729039153 1104 20-21 0.4545662698564641 1104 22-23 0.38807730096354476 1104 24-25 0.5003706107964803 1104 26-27 0.6159171114831494 1104 28-29 0.7075031596307397 1104 30-31 0.5938123629782126 1104 32-33 0.6177048607057216 1104 34-35 0.5871031630623449 1104 36-37 0.6546305111070936 1104 38-39 0.6573307980552414 1104 40-41 0.8133904189383045 1104 42-43 0.8199840144898829 1104 44-45 0.6241608807813535 1104 46-47 0.6479715270754127 1104 48-49 0.8187296538813484 1104 50-51 0.9245448381463888 1104 52-53 0.766362692556207 1104 54-55 0.8765551757665335 1104 56-57 0.9808292111106667 1104 58-59 0.8909998940660415 1104 60-61 0.8474654729756779 1104 62-63 0.9324015421635607 1104 64-65 0.9479644554880124 1104 66-67 1.0316772320811118 1104 68-69 1.1736586279231034 1104 70-71 1.384847136973633 1104 72-73 1.5400637603223863 1104 74-75 1.5767375043819527 1104 76-77 1.5622975138162722 1104 78-79 1.7221372196491878 1104 80-81 1.802709587861802 1104 82-83 2.0180657658731356 1104 84-85 2.0468657697835297 1104 86-87 2.1887361192028187 1104 88-89 2.6604348304628083 1104 90-91 2.5461962729700467 1104 92-93 2.867722698101854 1104 94-95 2.9655443479065973 1104 96-97 2.9924802854626193 1104 98-99 3.3392814955208756 1104 100-101 3.5301682700136556 1105 1 0.16344095942011805 1105 2 0.18098571518717677 1105 3 0.020219796822416924 1105 4 -0.017087109053946392 1105 5 0.03132746740910619 1105 6 0.17958010159782845 1105 7 0.21339482688416922 1105 8 0.09395015615661606 1105 9 0.17919998105357138 1105 10-11 0.18378801032043413 1105 12-13 0.14683307461324802 1105 14-15 0.2424512582502487 1105 16-17 0.45295524968700107 1105 18-19 0.32745805274525 1105 20-21 0.2326847670602632 1105 22-23 0.23250958653384401 1105 24-25 0.390696888595933 1105 26-27 0.39956298545895663 1105 28-29 0.47495199075311945 1105 30-31 0.3779454386382426 1105 32-33 0.4486575609918404 1105 34-35 0.42368179812541484 1105 36-37 0.42611666421543504 1105 38-39 0.506927218265929 1105 40-41 0.4965223688017417 1105 42-43 0.496305809424193 1105 44-45 0.6469094908086603 1105 46-47 0.5480225333831257 1105 48-49 0.4654195274019628 1105 50-51 0.4048611788603935 1105 52-53 0.43713831357325006 1105 54-55 0.5973814982727035 1105 56-57 0.5952149204031656 1105 58-59 0.4478932644653071 1105 60-61 0.5778188999492713 1105 62-63 0.7249262731103627 1105 64-65 0.6361990682465049 1105 66-67 0.9645627869321949 1105 68-69 1.0896790792171558 1105 70-71 0.989383650174311 1105 72-73 1.3025491290309326 1105 74-75 1.224709139997767 1105 76-77 1.3224083458490448 1105 78-79 1.6152642720942403 1105 80-81 1.5374762797268033 1105 82-83 1.7140590273505723 1105 84-85 1.9100316967571267 1105 86-87 2.108946301768821 1105 88-89 2.6502853880775845 1105 90-91 2.9064273051263747 1105 92-93 3.3180083131038067 1105 94-95 3.5079796654056246 1105 96-97 3.6685902314894108 1105 98-99 4.023641356198464 1105 100-101 3.957167270208741 1106 1 0.17822650325048173 1106 2 0.14530956048717059 1106 3 0.12891479148407825 1106 4 0.21754890070931765 1106 5 0.19615974063543007 1106 6 0.0934074659691646 1106 7 0.11834663057836536 1106 8 0.11804528450366547 1106 9 0.09533054510308858 1106 10-11 0.1305701847949976 1106 12-13 0.2811059863839134 1106 14-15 0.2746045609145753 1106 16-17 0.28371317326855916 1106 18-19 0.3090059904514888 1106 20-21 0.21838542066689826 1106 22-23 0.22058754144980242 1106 24-25 0.15763364814672798 1106 26-27 0.18030003960154062 1106 28-29 0.10575346794644958 1106 30-31 0.23028886491619716 1106 32-33 0.2575645770552697 1106 34-35 0.2586037796937646 1106 36-37 0.34917497463775504 1106 38-39 0.3367815556309992 1106 40-41 0.4919878026592315 1106 42-43 0.5491259057865676 1106 44-45 0.4993315555523168 1106 46-47 0.47380067612403565 1106 48-49 0.6758968931343432 1106 50-51 0.6511266078785951 1106 52-53 0.6076461668338666 1106 54-55 0.10003176579471784 1106 56-57 0.5136675876082037 1106 58-59 0.6777224980124785 1106 60-61 0.8480380593040735 1106 62-63 0.6616865800423426 1106 64-65 0.8849854650017548 1106 66-67 1.0232618647449634 1106 68-69 0.9009974228491018 1106 70-71 1.0129963617798339 1106 72-73 1.2560609414358446 1106 74-75 1.3975262420493237 1106 76-77 1.67054385807279 1106 78-79 1.9252374442792082 1106 80-81 2.0279920270795593 1106 82-83 2.1053466414897706 1106 84-85 2.132682145752522 1106 86-87 1.9125210249462974 1106 88-89 2.1889815623162256 1106 90-91 2.384559006409088 1106 92-93 2.7111341013788106 1106 94-95 2.8441863272934924 1106 96-97 3.017905320698702 1106 98-99 3.421868814935955 1106 100-101 2.8944818270961097 1107 1 0.36787842110006963 1107 2 0.16155155492683804 1107 3 0.16572004049862699 1107 4 0.1274384297848954 1107 5 0.23841883812708886 1107 6 0.24962915126061347 1107 7 0.07679564280644513 1107 8 0.21522426831055697 1107 9 0.3471872155644107 1107 10-11 0.23888241520025133 1107 12-13 0.15001953407653446 1107 14-15 0.24671215293495408 1107 16-17 0.4164082398299058 1107 18-19 0.24122566031650194 1107 20-21 0.3437239871391782 1107 22-23 0.4665261958186875 1107 24-25 0.48454268871856243 1107 26-27 0.46277338021440784 1107 28-29 0.51211261267769 1107 30-31 0.5472144296615298 1107 32-33 0.634445932134291 1107 34-35 0.6654154757496542 1107 36-37 0.6584899329352609 1107 38-39 0.6429545493039939 1107 40-41 0.6667636082489921 1107 42-43 0.7517904892650975 1107 44-45 0.6496744297323929 1107 46-47 0.5177501859165758 1107 48-49 0.4645759950401853 1107 50-51 0.3679412854490849 1107 52-53 0.3637028523963721 1107 54-55 0.4704885861061925 1107 56-57 0.6181411179538259 1107 58-59 0.6829111078522487 1107 60-61 0.7091662721764749 1107 62-63 0.9060567931625556 1107 64-65 1.0016529544890105 1107 66-67 1.0414057847784157 1107 68-69 1.1153731633876376 1107 70-71 1.225038195914692 1107 72-73 1.4884278961865185 1107 74-75 1.6073358123240098 1107 76-77 1.8963306681619265 1107 78-79 1.9951181449738655 1107 80-81 2.187133757442222 1107 82-83 2.1316006684705364 1107 84-85 2.2944697283249873 1107 86-87 2.3547536953766475 1107 88-89 2.60398346932395 1107 90-91 2.8216122632297846 1107 92-93 2.904057457092865 1107 94-95 3.4357655329715335 1107 96-97 3.388381259488593 1107 98-99 3.510965280485909 1107 100-101 3.6881118888947757 1108 1 0.2104935352509223 1108 2 0.05771308894238558 1108 3 0.045658796954583636 1108 4 0.053767410985756214 1108 5 0.047390294856462845 1108 6 0.20784608604531485 1108 7 0.09374552535815184 1108 8 0.09729710107455247 1108 9 0.20039536951430392 1108 10-11 0.2029939947872137 1108 12-13 0.21380887184597697 1108 14-15 0.3074793737451671 1108 16-17 0.46154038768879246 1108 18-19 0.3983055788807448 1108 20-21 0.22474668578241364 1108 22-23 0.17474451997598806 1108 24-25 0.1739572441563766 1108 26-27 0.30049095596300646 1108 28-29 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quality scores pass #Quality Count 2 3640.0 3 1031.0 4 1062.0 5 1217.0 6 1384.0 7 1789.0 8 2104.0 9 2759.0 10 3355.0 11 4350.0 12 5621.0 13 7372.0 14 8285.0 15 10680.0 16 13609.0 17 17805.0 18 16933.0 19 12693.0 20 12547.0 21 13313.0 22 14323.0 23 16465.0 24 18064.0 25 20563.0 26 23371.0 27 27392.0 28 31512.0 29 37068.0 30 43610.0 31 53394.0 32 65283.0 33 82924.0 34 111007.0 35 156525.0 36 190900.0 37 210715.0 38 109249.0 39 5819.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.124052428590424 18.35399642398002 14.403898157315325 22.118052990114226 2 15.853830805360833 20.189936373963356 40.03231331555806 23.92391950511775 3 19.196151321695467 25.279099816631494 29.467510220660305 26.057238641012738 4 12.758788412165917 16.858416294021165 36.178169314123785 34.20462597968913 5 13.655233633990834 37.70295117394806 33.01518205064413 15.626633141416981 6 31.738179323624276 35.7559359161015 17.163552908374395 15.342331851899832 7 28.67723559648526 33.696079253729366 19.7699469787606 17.85673817102477 8 27.44206784110373 34.00118895566137 19.047202128348353 19.50954107488655 9 27.735167128254783 16.510482121676787 19.682195844823983 36.072154905244446 10-11 25.618209845884426 26.78557369329198 25.817283460076744 21.778933000746846 12-13 26.733161724481334 24.505839716547403 26.87362247842964 21.887376080541625 14-15 23.430845056640475 25.345702982738942 26.066427945529203 25.157024015091373 16-17 21.989523017724053 28.923313492793394 26.47906442902654 22.608099060456013 18-19 21.927778217209834 26.566255419576883 30.090447380668138 21.415518982545144 20-21 22.889905691478088 25.556093554995645 30.354782831374948 21.199217922151316 22-23 22.603514072218786 25.541239891588308 30.384852677751873 21.47039335844103 24-25 22.09797584585681 25.864604771291443 30.432189235543188 21.605230147308554 26-27 21.72265007125094 26.2534222709861 30.311984543716214 21.711943114046754 28-29 21.67616246066821 26.332443167792462 30.47658744450874 21.514806927030584 30-31 21.780944129587425 26.098709975295836 30.39105425903137 21.729291636085375 32-33 21.523767549608696 26.325286777427753 30.223090599998603 21.927855072964945 34-35 22.01069281053446 26.303232272097148 30.077098313226063 21.608976604142324 36-37 22.077188691725695 25.99011412607212 29.993907782366563 21.938789399835628 38-39 21.706980916863056 26.29210540544815 30.133266732458324 21.867646945230465 40-41 21.816357449694696 26.27715909044932 30.22485709420031 21.681626365655674 42-43 21.922804457035163 26.17360574203368 29.802321501214895 22.101268299716267 44-45 21.670874623720373 26.455588430539283 29.798257115063876 22.075279830676468 46-47 21.945635781807482 26.68404016625593 29.342774119983815 22.027549931952773 48-49 21.995867124142233 26.82253351616662 29.024845807977968 22.15675355171318 50-51 22.011779217999386 26.94806890408551 28.86798434439391 22.172167533521193 52-53 22.240206543865746 26.936394713565658 28.70882500593041 22.11457373663818 54-55 21.971521116058607 26.9587277955805 28.718898917505243 22.35085217085565 56-57 22.024019248352143 27.234932434370755 28.546487550873746 22.194560766403356 58-59 22.163499890217437 26.821259641833006 28.583885832017597 22.431354635931964 60-61 22.07548273177212 26.94842463256996 28.847685119970812 22.128407515687105 62-63 21.95903887323985 27.007664902426132 28.878819073216533 22.15447715111748 64-65 21.915052314427953 26.886247013546438 28.800997740299174 22.397702931726435 66-67 21.943065269844027 27.10899710541887 28.639877847278942 22.30805977745816 68-69 21.90331254084545 26.84636152302369 28.762148821058215 22.488177115072645 70-71 21.871822078703033 26.91096395490179 28.658069000774194 22.559144965620984 72-73 21.915858462149547 27.037135112670512 28.4005457909003 22.64646063427964 74-75 21.738959340037358 27.316799941744634 28.375289575857565 22.56895114236044 76-77 21.87572976775779 27.002519503072058 28.478916676197702 22.642834052972454 78-79 21.91182355000721 27.04674577278518 28.30498027250714 22.73645040470047 80-81 21.806603235784138 27.381398102958105 28.224585045072214 22.587413616185543 82-83 22.055586550983637 27.180779679879546 28.1203395758575 22.643294193279317 84-85 21.88937405919846 27.137745665739025 28.05169080402237 22.921189471040137 86-87 22.089352346077344 26.959452725981652 28.197938565895246 22.753256362045754 88-89 21.92897049406089 26.92968223835673 28.097421057994104 23.04392620958828 90-91 21.954613521923164 27.023762186125538 28.10620905235597 22.91541523959533 92-93 22.13995689560056 26.676694937071986 28.12896748045223 23.054380686875227 94-95 21.914709773912815 26.585025195388873 28.191468034649315 23.308796996049 96-97 22.228450657185714 26.43433623396944 28.057383012703802 23.279830096141044 98-99 22.06255519473235 26.510611549358664 27.848710250137653 23.57812300577133 100-101 22.79688216414489 25.55487424249957 27.938512417390793 23.709731175964748 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3741.0 1 2428.5 2 1699.0 3 2128.5 4 1558.0 5 1332.5 6 2423.0 7 4972.0 8 7632.0 9 8190.0 10 5925.0 11 3527.0 12 3001.0 13 3071.0 14 3047.5 15 2948.5 16 2873.5 17 2863.5 18 2899.5 19 2994.5 20 3134.0 21 3405.5 22 3849.5 23 4415.5 24 5085.0 25 5888.5 26 6837.0 27 7864.0 28 8973.0 29 10497.0 30 12588.0 31 14731.5 32 17255.0 33 19588.5 34 21476.0 35 23376.5 36 26182.5 37 29257.0 38 31426.0 39 32861.5 40 34206.5 41 36746.0 42 39077.0 43 41540.5 44 44892.0 45 48239.5 46 49454.5 47 49932.5 48 51550.5 49 51475.0 50 50474.0 51 48901.0 52 47207.5 53 46665.5 54 46175.0 55 44235.5 56 40875.0 57 37639.5 58 34561.0 59 30862.5 60 26559.5 61 22192.0 62 18113.0 63 14111.0 64 10889.0 65 8765.0 66 6905.5 67 5527.0 68 4298.0 69 3045.5 70 2186.0 71 1613.0 72 1238.0 73 988.0 74 750.0 75 589.5 76 430.0 77 260.5 78 152.5 79 110.0 80 76.0 81 41.5 82 26.0 83 20.5 84 17.5 85 9.0 86 7.0 87 7.0 88 3.5 89 3.5 90 4.0 91 2.5 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4613405720093577 2 0.03978722293273753 3 0.053025115960265726 4 0.05559915071561844 5 0.08494314692663928 6 0.08376644532419233 7 0.04817122185017205 8 0.04096392453518448 9 0.048318309550477924 10-11 0.0505981689052189 12-13 0.0466635729220369 14-15 0.0401549421835022 16-17 0.04140518763610209 18-19 0.04820799377524852 20-21 0.05802609777066527 22-23 0.048134449925095586 24-25 0.06755002636547028 26-27 0.05909248359788282 28-29 0.04578104672020169 30-31 0.04784027452448385 32-33 0.04570750287004875 34-35 0.05067171275537183 36-37 0.045744274795125224 38-39 0.02879241733487383 40-41 0.026365470279826993 42-43 0.02603452295413879 44-45 0.015113261206428025 46-47 0.028167294608573888 48-49 0.0286085577094915 50-51 0.016179647033645574 52-53 0.01584869970795737 54-55 0.017246032860863125 56-57 0.017650524036704265 58-59 0.016731225909792584 60-61 0.018863997564227686 62-63 0.01651059435933378 64-65 0.01919494488991589 66-67 0.009082665493887402 68-69 0.013789471903675207 70-71 0.01879045371407475 72-73 0.017503436336398396 74-75 0.014819085805816288 76-77 0.010259367096334354 78-79 0.015370664681963296 80-81 0.01823887483792774 82-83 0.015701612007651505 84-85 0.013789471903675207 86-87 0.00356687673241732 88-89 0.029160136585638506 90-91 0.0332050483440499 92-93 0.017871155587163068 94-95 0.033572767594814576 96-97 0.027652487657503347 98-99 0.02511522482722711 100-101 0.1515371032401214 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1359733.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.990841886241206 #Duplication Level Percentage of deduplicated Percentage of total 1 77.24822705180236 42.47945039798124 2 12.770753734196399 14.045489987306375 3 4.174483009409793 6.88675005381763 4 1.8658387849672258 4.104161824373965 5 1.0340877838767835 2.843267890983088 6 0.6155092064993944 2.0308421672480392 7 0.414164525534408 1.5942679156986919 8 0.2953637241016274 1.2993839880803173 9 0.21915519932779506 1.084637602330421 >10 1.2274253380630928 12.495737301755018 >50 0.09270500900619691 3.4907203871051466 >100 0.03960178561000921 3.8448369856753053 >500 0.00134242380247679 0.4338461055448088 >1k 9.396966617337531E-4 1.0519494646951721 >5k 1.3424238024767899E-4 0.5250888801667901 >10k+ 2.6848476049535797E-4 1.78956904723792 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13585 0.9990932043276143 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10657 0.7837568110798223 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7113 0.5231174061378226 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3632 0.2671112637554579 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2368 0.17415183716214874 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2354 0.17312222326000767 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2020 0.14855857730892755 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1374 0.10104925011013191 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 7.354385015293443E-5 0.0 22-23 0.0 0.0 0.0 3.677192507646722E-4 0.0 24-25 0.0 0.0 0.0 8.089823516822788E-4 0.0 26-27 0.0 0.0 0.0 0.0027946663058115084 0.0 28-29 0.0 0.0 0.0 0.007611788490828714 0.0 30-31 0.0 0.0 0.0 0.02180575157034506 0.0 32-33 0.0 0.0 0.0 0.04618553789604282 0.0 34-35 0.0 0.0 0.0 0.08130272634406902 0.0 36-37 0.0 0.0 0.0 0.13815212251228734 0.0 38-39 0.0 0.0 0.0 0.2171749895016154 0.0 40-41 0.0 0.0 0.0 0.30410382038238387 0.0 42-43 0.0 0.0 0.0 0.38809089725703505 0.0 44-45 0.0 0.0 0.0 0.49189804174790197 0.0 46-47 0.0 0.0 0.0 0.6122157805981028 0.0 48-49 0.0 0.0 0.0 0.73871120286115 0.0 50-51 0.0 0.0 0.0 0.870943045436126 0.0 52-53 0.0 0.0 0.0 0.9931361524652267 0.0 54-55 0.0 0.0 0.0 1.1228307322099265 0.0 56-57 0.0 0.0 0.0 1.278706922609071 0.0 58-59 0.0 0.0 0.0 1.4441070416030206 0.0 60-61 0.0 0.0 0.0 1.615390668609205 0.0 62-63 0.0 0.0 0.0 1.7852769624624834 0.0 64-65 0.0 0.0 0.0 1.9580314664717264 0.0 66-67 0.0 0.0 0.0 2.1548348094809793 0.0 68-69 0.0 0.0 0.0 2.370465378129383 0.0 70-71 0.0 0.0 0.0 2.595509559597362 0.0 72-73 0.0 0.0 0.0 2.8289745119078527 0.0 74-75 0.0 0.0 0.0 3.0519962374966263 0.0 76-77 0.0 0.0 0.0 3.2917124170701157 0.0 78-79 0.0 0.0 0.0 3.551358980035051 0.0 80-81 0.0 0.0 0.0 3.8323700314694134 0.0 82-83 0.0 0.0 0.0 4.120919327544452 0.0 84-85 0.0 0.0 0.0 4.403805747157714 0.0 86-87 0.0 0.0 0.0 4.693347885209817 0.0 88-89 0.0 3.6771925076467215E-5 0.0 5.004622230982112 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4485 0.0 54.812515 1 GTATCAA 8050 0.0 43.82112 1 TATCAAC 10505 0.0 33.571285 2 ATCAACG 10545 0.0 33.353794 3 TCAACGC 10645 0.0 33.04047 4 CAACGCA 10835 0.0 32.32948 5 AACGCAG 11115 0.0 31.552013 6 GTGGTAT 1930 0.0 28.443005 1 ACGCAGA 12610 0.0 27.689083 7 CGCAGAG 12700 0.0 27.305836 8 TGGTATC 1985 0.0 26.338577 2 GTACATG 8535 0.0 25.727007 1 GCAGAGT 13420 0.0 25.237213 9 TACATGG 8660 0.0 24.972017 2 GAGTACT 7865 0.0 24.582386 12-13 ACATGGG 8755 0.0 23.560999 3 GTACTTT 8330 0.0 23.127151 14-15 CATGGGG 3645 0.0 22.167234 4 GTATAAC 280 6.184564E-11 22.16258 1 GTATAGG 455 0.0 22.031439 1 >>END_MODULE