FastQCFastQC Report
Thu 26 May 2016
SRR938218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938218_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359733
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131590.9677635241624643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108930.8011131597159148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74320.5465778943366087No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36240.2665229129542344No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25120.1847421515841713No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24450.1798147136239247No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19340.14223380619577522No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47750.053.2229461
GTATCAA80050.038.9279021
ATCAACG107000.028.9011883
TATCAAC107850.028.8066042
TCAACGC109000.028.5431164
CAACGCA110350.028.1078365
AACGCAG112850.027.4420536
TACCGTC1351.3435156E-524.6283237
GTACATG85850.024.2355181
TGGTATC22200.024.1802022
ACGCAGA128250.024.0357637
GTGGTAT22650.023.9086531
CGCAGAG129450.023.776268
TACATGG87350.023.3307572
ATACCGT1903.2895332E-722.4988066
GAGTACT81450.022.47956512-13
ACATGGG87450.022.1081853
GCAGAGT137550.022.064549
GTACTTT84650.021.65783114-15
AGTACTT83200.021.20753112-13
Produced by FastQC (version 0.11.5)