##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938218_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1359733 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49228635327671 33.0 31.0 34.0 31.0 34.0 2 32.714938153299215 34.0 31.0 34.0 31.0 34.0 3 32.66692431528837 34.0 31.0 34.0 31.0 34.0 4 36.205454306102745 37.0 35.0 37.0 35.0 37.0 5 36.17186315254539 37.0 35.0 37.0 35.0 37.0 6 36.240294969674196 37.0 37.0 37.0 35.0 37.0 7 36.199707589651794 37.0 37.0 37.0 35.0 37.0 8 36.19969214544326 37.0 37.0 37.0 35.0 37.0 9 37.890168878743104 39.0 38.0 39.0 35.0 39.0 10-11 37.85932311711196 39.0 38.0 39.0 35.0 39.0 12-13 37.79345724491499 39.0 38.0 39.0 35.0 39.0 14-15 39.030053694364994 40.0 38.0 41.0 36.0 41.0 16-17 38.98102164174878 40.0 38.0 41.0 35.5 41.0 18-19 39.002588743525386 40.0 38.5 41.0 35.5 41.0 20-21 39.02147701056016 40.0 38.5 41.0 35.0 41.0 22-23 39.02715864070373 40.0 39.0 41.0 35.0 41.0 24-25 38.96818088551208 40.0 38.0 41.0 35.0 41.0 26-27 38.7926677516836 40.0 38.0 41.0 35.0 41.0 28-29 38.7018988286671 40.0 38.0 41.0 35.0 41.0 30-31 38.44985669980798 40.0 38.0 41.0 34.0 41.0 32-33 38.20612392285838 40.0 37.0 41.0 34.0 41.0 34-35 37.98039357726848 40.0 37.0 41.0 33.5 41.0 36-37 37.89530518123779 40.0 37.0 41.0 33.0 41.0 38-39 37.84569176448612 40.0 37.0 41.0 33.0 41.0 40-41 37.70226948967187 40.0 37.0 41.0 33.0 41.0 42-43 37.48536550925807 40.0 36.5 41.0 33.0 41.0 44-45 37.18199381790396 40.0 36.0 41.0 32.5 41.0 46-47 36.74696650004081 40.0 36.0 41.0 31.0 41.0 48-49 36.32346129718114 39.0 35.0 41.0 30.0 41.0 50-51 36.01690589255391 39.0 35.0 41.0 29.5 41.0 52-53 35.56668110577591 39.0 35.0 40.0 28.0 41.0 54-55 35.25738325097648 38.0 35.0 40.0 27.5 41.0 56-57 34.99645739273814 38.0 34.5 40.0 27.0 41.0 58-59 34.619499195798 38.0 34.0 40.0 26.0 41.0 60-61 34.21765927575487 37.0 34.0 40.0 26.0 41.0 62-63 33.793405764219884 36.5 33.0 39.5 24.5 41.0 64-65 33.3594602763925 36.0 33.0 39.0 23.0 40.5 66-67 32.85553744742534 35.5 32.5 39.0 22.0 40.0 68-69 32.341287223300455 35.0 32.0 38.0 20.5 40.0 70-71 31.765397324327644 35.0 31.0 37.5 18.5 39.5 72-73 31.306091342932767 35.0 31.0 37.0 17.5 39.0 74-75 30.76699653534922 34.5 30.0 36.0 13.0 39.0 76-77 29.570283283556403 33.5 28.5 35.0 12.5 37.0 78-79 30.27209091784931 34.0 30.0 35.0 9.5 37.0 80-81 30.095298856466673 34.0 30.0 35.0 7.0 37.0 82-83 29.66998778436649 34.0 30.0 35.0 4.0 36.5 84-85 29.183315400891203 34.0 29.0 35.0 2.0 36.0 86-87 28.6576585256076 34.0 29.0 35.0 2.0 36.0 88-89 28.071093736785087 33.5 27.5 35.0 2.0 35.0 90-91 27.570736681392596 33.0 26.5 35.0 2.0 35.0 92-93 26.95457600867229 33.0 25.5 35.0 2.0 35.0 94-95 26.233516433005597 32.5 24.5 34.5 2.0 35.0 96-97 25.436155480524484 32.0 21.5 34.0 2.0 35.0 98-99 24.449841623318697 32.0 10.0 34.0 2.0 35.0 100-101 21.04113417854829 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.057165873879611695 1101 2 -0.04045221814607913 1101 3 0.15757164563451198 1101 4 -0.02876924392315061 1101 5 -0.11041034601235111 1101 6 0.015685400545592643 1101 7 0.010763233819190532 1101 8 0.08185621378112273 1101 9 0.033789553748000856 1101 10-11 0.06433402883303074 1101 12-13 0.008910984412388245 1101 14-15 0.0875147779661063 1101 16-17 0.25022150864440107 1101 18-19 0.2090706414556962 1101 20-21 0.189894677445686 1101 22-23 0.17497798297077338 1101 24-25 0.168882831184618 1101 26-27 0.21909315621758196 1101 28-29 0.19626141182846624 1101 30-31 0.1483490450960474 1101 32-33 0.26487153711623534 1101 34-35 0.09263722515258621 1101 36-37 0.18628943514062968 1101 38-39 0.27583555784499225 1101 40-41 0.22266335738804344 1101 42-43 0.21326321672430737 1101 44-45 0.34871367457881064 1101 46-47 0.17297856309381388 1101 48-49 0.1776853879903726 1101 50-51 0.21570275335091083 1101 52-53 0.26196327226652016 1101 54-55 0.17348547565048733 1101 56-57 0.15432804664423827 1101 58-59 0.20468926937437715 1101 60-61 0.34989654582736307 1101 62-63 0.3610146654970947 1101 64-65 0.22880309325740456 1101 66-67 0.281277966176809 1101 68-69 0.42167077734509206 1101 70-71 0.3524104160386763 1101 72-73 0.36470471092425427 1101 74-75 0.5249058414331564 1101 76-77 0.5032335448078413 1101 78-79 0.6945823470316661 1101 80-81 0.8392449801205544 1101 82-83 0.9712313406735156 1101 84-85 1.13362174728611 1101 86-87 1.1988969630753061 1101 88-89 1.3885103536517285 1101 90-91 1.5962998168922162 1101 92-93 1.9027791558310625 1101 94-95 1.82057727646826 1101 96-97 2.013425950868694 1101 98-99 2.3181056224640315 1101 100-101 2.5538807964491888 1102 1 0.014653219357718683 1102 2 0.14528257636469988 1102 3 0.2735491870162079 1102 4 0.12155726206155038 1102 5 0.04411719373458567 1102 6 0.09571310228229635 1102 7 0.2048555115142392 1102 8 0.1310220407760525 1102 9 0.010873202887083266 1102 10-11 0.2035058301295436 1102 12-13 0.171901226386467 1102 14-15 0.2745493546080695 1102 16-17 0.2737338035192849 1102 18-19 0.20953342690693688 1102 20-21 0.14802552356729137 1102 22-23 0.235911103488192 1102 24-25 0.15304171788392296 1102 26-27 0.2921573784734903 1102 28-29 0.1253286182628841 1102 30-31 0.19150282242996752 1102 32-33 0.25681811913210595 1102 34-35 0.24902274296997007 1102 36-37 0.25295729072303175 1102 38-39 0.5213921338865646 1102 40-41 0.4495830847696709 1102 42-43 0.32963666704694816 1102 44-45 0.26045243412357166 1102 46-47 0.27554929614952783 1102 48-49 0.24189189076499673 1102 50-51 0.2668466389058821 1102 52-53 0.35552677968684065 1102 54-55 0.29255390072353293 1102 56-57 0.34929477186500435 1102 58-59 0.28132499450894954 1102 60-61 0.3184167321304514 1102 62-63 0.4446956080694875 1102 64-65 0.4058703948050706 1102 66-67 0.4578295755228723 1102 68-69 0.5339490074062354 1102 70-71 0.6576223254076332 1102 72-73 0.6042950105607439 1102 74-75 0.7710714395618048 1102 76-77 0.663845955304776 1102 78-79 0.7704170401873789 1102 80-81 0.875197494825958 1102 82-83 0.8437266667485162 1102 84-85 0.9239641847417097 1102 86-87 1.0515344240268547 1102 88-89 1.3031860322996955 1102 90-91 1.3741081101739532 1102 92-93 1.6945851485608436 1102 94-95 1.7203183900661863 1102 96-97 1.734096737128393 1102 98-99 2.239553365158887 1102 100-101 2.3353613532184276 1103 1 0.2509397720013311 1103 2 0.29624597072601944 1103 3 0.39857289533141227 1103 4 0.20329014465797712 1103 5 0.10356760709603208 1103 6 0.18463323036298362 1103 7 0.18063621238781735 1103 8 0.154980269513878 1103 9 0.1854244184263436 1103 10-11 0.36870983320883965 1103 12-13 0.2928906162181093 1103 14-15 0.4413655231211919 1103 16-17 0.4258151146727869 1103 18-19 0.373732197421603 1103 20-21 0.3147468588196034 1103 22-23 0.4731537985378935 1103 24-25 0.5066068906478023 1103 26-27 0.5049118942102311 1103 28-29 0.5399429815970009 1103 30-31 0.5254609395992418 1103 32-33 0.5450554477349314 1103 34-35 0.7333027353833188 1103 36-37 0.6803953422900122 1103 38-39 0.7462998109179466 1103 40-41 0.9154486893038154 1103 42-43 0.8316281655240303 1103 44-45 0.8577357401226138 1103 46-47 1.0135004514417858 1103 48-49 0.9832157630859584 1103 50-51 0.7704525341876476 1103 52-53 0.8010601331025171 1103 54-55 0.9261014456577641 1103 56-57 0.8630087770640955 1103 58-59 0.8938318280821633 1103 60-61 1.0600396081443435 1103 62-63 0.9594683987334349 1103 64-65 0.956167724969859 1103 66-67 1.0231763863360968 1103 68-69 0.9929631877679412 1103 70-71 1.0769828145241185 1103 72-73 1.1359057256035285 1103 74-75 1.1732224810541432 1103 76-77 0.7043843850891172 1103 78-79 1.129749190894163 1103 80-81 1.2429642273180619 1103 82-83 1.2681947637238942 1103 84-85 1.3924973931182762 1103 86-87 1.6752795568492047 1103 88-89 1.7207413424044589 1103 90-91 1.5728763934687926 1103 92-93 1.8446738455518386 1103 94-95 1.67792903816785 1103 96-97 1.9193797307355176 1103 98-99 2.3814484684155772 1103 100-101 2.8028558584677263 1104 1 0.15885177244050652 1104 2 0.047518890137531855 1104 3 0.1323577547956205 1104 4 -9.152663061229305E-6 1104 5 6.752322607255223E-4 1104 6 0.07854517590536148 1104 7 -0.026661630168177908 1104 8 -0.023274424162011087 1104 9 0.046250066208514795 1104 10-11 0.05938218169369236 1104 12-13 0.017406838220743737 1104 14-15 0.14659581517214093 1104 16-17 -0.011888003465109875 1104 18-19 0.006377262199819711 1104 20-21 0.18841252361978178 1104 22-23 0.16009693918348233 1104 24-25 0.08058924132852496 1104 26-27 0.10472490200557161 1104 28-29 0.06637647413103309 1104 30-31 0.2285893706488764 1104 32-33 0.12147006949602002 1104 34-35 0.08870629544040298 1104 36-37 0.0664340582540035 1104 38-39 0.026015445524883773 1104 40-41 0.11903516547860704 1104 42-43 0.3187514362750221 1104 44-45 0.17344685946824256 1104 46-47 0.3855229865757366 1104 48-49 0.15765341405214173 1104 50-51 0.15209436005501686 1104 52-53 0.41020662519736817 1104 54-55 0.29798776812152994 1104 56-57 0.30855047117916 1104 58-59 0.35599043864430513 1104 60-61 0.25360261593968403 1104 62-63 0.329014402246834 1104 64-65 0.17423597540903302 1104 66-67 0.08161196494830136 1104 68-69 0.36698053515485185 1104 70-71 0.4440546881516987 1104 72-73 0.424140313328607 1104 74-75 0.35070656098387687 1104 76-77 0.4202814673245143 1104 78-79 0.6610324720755436 1104 80-81 0.7321536761542511 1104 82-83 0.7274407562579306 1104 84-85 0.8953396746290352 1104 86-87 0.7896453616362038 1104 88-89 0.8360421082147376 1104 90-91 0.8251964692263698 1104 92-93 1.035738363009024 1104 94-95 1.0495010764858108 1104 96-97 1.2213632205247116 1104 98-99 1.4329049489821095 1104 100-101 1.9014813799872705 1105 1 0.06770492304526954 1105 2 0.14816693442157458 1105 3 0.15961869575023258 1105 4 0.1316805989301244 1105 5 0.011964696105174255 1105 6 0.0679862605717716 1105 7 -0.04110291804696686 1105 8 -0.029867649874070423 1105 9 0.07954438276463804 1105 10-11 0.10139938971813933 1105 12-13 0.017245284377594317 1105 14-15 0.0675157169799192 1105 16-17 0.0698521447986451 1105 18-19 0.05946751288261254 1105 20-21 0.13777250622479897 1105 22-23 0.17974706842655053 1105 24-25 0.034137939143590756 1105 26-27 0.04612708775795227 1105 28-29 0.19713856120347373 1105 30-31 0.042545387361059284 1105 32-33 0.12180587795616304 1105 34-35 0.08537920072717498 1105 36-37 0.17430071435362748 1105 38-39 0.1253050150397712 1105 40-41 0.2175987587612127 1105 42-43 -0.06312831889470516 1105 44-45 0.1163121743661506 1105 46-47 0.10621468087928321 1105 48-49 0.29251683501065173 1105 50-51 0.2411674270988442 1105 52-53 0.17603991788822526 1105 54-55 0.259590280639415 1105 56-57 0.38071626809683323 1105 58-59 0.20015245174743512 1105 60-61 0.292241926155576 1105 62-63 0.4160502651678897 1105 64-65 0.5191417458278096 1105 66-67 0.4962414274922011 1105 68-69 0.3632820698147583 1105 70-71 0.4635402153658994 1105 72-73 0.44061016880396053 1105 74-75 0.3502310440115828 1105 76-77 0.2815702199256336 1105 78-79 0.5778002369019184 1105 80-81 0.7585651893549255 1105 82-83 0.7529296852473983 1105 84-85 1.0423211579168488 1105 86-87 1.0914817919262276 1105 88-89 1.3786501562378852 1105 90-91 1.309357080164073 1105 92-93 1.440201618146233 1105 94-95 1.5190146470086354 1105 96-97 1.6657130018681912 1105 98-99 2.0174875304296336 1105 100-101 2.269054983511783 1106 1 0.11006081049547589 1106 2 0.06902941691695474 1106 3 0.16764889292105778 1106 4 0.021616992509592592 1106 5 0.05144271816629242 1106 6 0.04573157477781109 1106 7 0.0656624096485956 1106 8 0.07189052474566893 1106 9 0.1163698688864585 1106 10-11 0.09943569649284001 1106 12-13 -0.06124119736769984 1106 14-15 -0.02862661259389654 1106 16-17 0.15601192606273173 1106 18-19 0.09183026607578881 1106 20-21 -0.029294158022224792 1106 22-23 0.14024636219264153 1106 24-25 0.171768108488493 1106 26-27 0.21326029224518095 1106 28-29 0.08886700443406426 1106 30-31 0.14465592279827888 1106 32-33 0.09658183394281394 1106 34-35 -0.07262816104303482 1106 36-37 0.10182705067824571 1106 38-39 0.18937872952770363 1106 40-41 0.1379773122368846 1106 42-43 0.30399082140539946 1106 44-45 0.25485046676211454 1106 46-47 -0.07654867480552241 1106 48-49 0.12485357701901734 1106 50-51 0.10648015110505327 1106 52-53 0.05633175733267848 1106 54-55 0.09122078873463835 1106 56-57 0.10895458499170729 1106 58-59 -0.010572171468460567 1106 60-61 -0.02970924540633746 1106 62-63 -0.0225315808398463 1106 64-65 0.1405882701588581 1106 66-67 0.15975348186162108 1106 68-69 0.009994981948366899 1106 70-71 0.15443887155550584 1106 72-73 0.018025201454044293 1106 74-75 0.18406191686830553 1106 76-77 0.2503802384661604 1106 78-79 0.639816496916982 1106 80-81 0.8140665549421264 1106 82-83 0.7612343609090928 1106 84-85 0.8512395160666664 1106 86-87 0.9873803422563583 1106 88-89 1.1693947112802725 1106 90-91 1.1298179015730945 1106 92-93 1.1013242729808326 1106 94-95 1.4619972225393703 1106 96-97 1.5505590670715783 1106 98-99 1.818302345916571 1106 100-101 2.2148677876687337 1107 1 0.06780219701592927 1107 2 0.08324098054711726 1107 3 0.13377820934107376 1107 4 0.1118789592250522 1107 5 0.07858950548749988 1107 6 0.07611011097029774 1107 7 0.13495019550614984 1107 8 0.030601387526296264 1107 9 -0.020245937787485957 1107 10-11 0.027546829545833873 1107 12-13 0.11925030725171837 1107 14-15 0.20954848749981636 1107 16-17 0.2076112472841416 1107 18-19 0.18113219476725106 1107 20-21 0.17496113126213686 1107 22-23 0.1457011786939688 1107 24-25 0.1367752428270279 1107 26-27 0.268127905252328 1107 28-29 0.13164714096419772 1107 30-31 0.09317010241710477 1107 32-33 0.26319162918633054 1107 34-35 0.3008808396461973 1107 36-37 0.3847524586036499 1107 38-39 0.38674846250789585 1107 40-41 0.28778438500907555 1107 42-43 0.3214752749363612 1107 44-45 0.2312055070706336 1107 46-47 0.2664779690932164 1107 48-49 0.19495595526093012 1107 50-51 0.10249552139367779 1107 52-53 0.22294857075181795 1107 54-55 0.25064058405559564 1107 56-57 0.2039753587915527 1107 58-59 0.38678386396897935 1107 60-61 0.4623352895161048 1107 62-63 0.7421013153337412 1107 64-65 0.5428790510833608 1107 66-67 0.498281233180073 1107 68-69 0.7057979677222832 1107 70-71 0.7058983132765739 1107 72-73 0.548028144247688 1107 74-75 0.7353843831616977 1107 76-77 0.6861523152266109 1107 78-79 0.6560335647328834 1107 80-81 0.8132444603700328 1107 82-83 0.8611351868273616 1107 84-85 1.0452678714322339 1107 86-87 1.1131733961017396 1107 88-89 1.2450276539190277 1107 90-91 1.1847353678277663 1107 92-93 1.218629690431598 1107 94-95 1.416036884427868 1107 96-97 1.2926786238713674 1107 98-99 1.3285210516294619 1107 100-101 1.708510288578676 1108 1 0.04332561998540996 1108 2 0.06114347252580643 1108 3 0.16214132464788378 1108 4 -0.03693905519478591 1108 5 -0.054867605506700556 1108 6 -0.003915898084706271 1108 7 -0.08976553886063243 1108 8 7.687158371538771E-5 1108 9 -0.07215243486425038 1108 10-11 -0.06860192224182526 1108 12-13 -0.02915830537638442 1108 14-15 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-2.9345738136030235 2316 100-101 -3.348337708700914 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 4.0 4 13.0 5 20.0 6 65.0 7 170.0 8 331.0 9 666.0 10 992.0 11 1790.0 12 2782.0 13 4091.0 14 5510.0 15 7494.0 16 10334.0 17 15009.0 18 15013.0 19 10138.0 20 8555.0 21 8692.0 22 8888.0 23 9980.0 24 11330.0 25 13069.0 26 15445.0 27 18587.0 28 22332.0 29 27211.0 30 33468.0 31 40864.0 32 51691.0 33 68235.0 34 96422.0 35 150288.0 36 212529.0 37 289849.0 38 186368.0 39 11502.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.28075217708182 18.887972859377538 14.770473320865198 22.060801642675436 2 16.02679615592256 20.46568176769898 39.587289772418025 23.920232303960436 3 19.45438177389176 25.323102133518482 29.53357967450016 25.688936418089604 4 12.944305977717685 17.235442546441103 35.9370552895311 33.8831961863101 5 13.66456110004273 37.74685985222055 33.071295824388535 15.517283223348183 6 31.6901651870848 35.849885014021154 17.055064567918212 15.404885230975832 7 28.67357047302669 33.799429740985914 19.659080128231057 17.86791965775634 8 27.388418860242638 34.29965404845675 18.908414035748326 19.40351305555229 9 27.35500278363473 16.592448664553995 19.690777527646972 36.361771024164305 10-11 25.571086382400075 26.913335191541282 25.759248323016358 21.756330103042288 12-13 26.554808922045726 24.497530029792614 26.947753713412855 21.99990733474881 14-15 23.274753205224847 25.435765698118672 26.01830653518007 25.271174561476407 16-17 21.890805033046927 28.967083978987052 26.564259306790376 22.57785168117564 18-19 21.782989748722727 26.63221382433169 30.094069938730616 21.490726488214964 20-21 22.681474966041126 25.60576230774718 30.385487444961623 21.327275281250067 22-23 22.476986290690892 25.629259567871042 30.484514239192546 21.409239902245513 24-25 22.065808150267337 25.95796162416986 30.507314059615652 21.46891616594715 26-27 21.65463786521784 26.29241113380419 30.43049537476861 21.622455626209362 28-29 21.57907432889102 26.31142133678305 30.634090519641077 21.47541381468486 30-31 21.66938043673312 26.158904843013183 30.421546304712987 21.75016841554071 32-33 21.41637848738606 26.309787700983904 30.38503007311982 21.888803738510216 34-35 21.953220766104934 26.36535054635353 30.156670014678046 21.52475867286349 36-37 21.951939759948868 26.0260718564724 30.09608517630203 21.9259032072767 38-39 21.666273124292452 26.22205941092223 30.3110382383425 21.800629226442812 40-41 21.771470017706093 26.172541357741803 30.356571447066727 21.69941717748538 42-43 21.869044142580883 26.15148722196191 29.920572630290533 22.058896005166677 44-45 21.67431905398473 26.360411813054967 29.94051548344271 22.024753649517596 46-47 21.922602272259255 26.66624500626607 29.364185146178873 22.0469675752958 48-49 21.962836766978175 26.79798430719017 29.112670079596626 22.126508846235026 50-51 21.94691899071362 26.94996002891744 28.902696338178156 22.200424642190782 52-53 22.238888075820768 26.926278909168193 28.721226887925795 22.113606127085244 54-55 21.94096193885123 26.96676479867739 28.73064050074537 22.361632761726018 56-57 21.99060403770446 27.227955782495535 28.597599675818707 22.183840503981298 58-59 22.08253385039563 26.884689861906715 28.603997990782016 22.428778296915645 60-61 21.995457931814556 27.08340534501994 28.824298593915127 22.096838129250376 62-63 21.894886716730415 27.192434102871665 28.80800863110625 22.104670549291665 64-65 21.811966025682985 26.95790276473396 28.746011165427333 22.484120044155727 66-67 21.853585996889816 27.22904201690561 28.566038832073946 22.35133315413063 68-69 21.86530825673095 27.040501512435956 28.65174406333036 22.44244616750274 70-71 21.807553394673807 27.125803374633108 28.474193095262084 22.592450135431 72-73 21.881979771028575 27.1750409823105 28.289781891003603 22.653197355657323 74-75 21.756881681918436 27.500619606937537 28.080292233842968 22.66220647730106 76-77 21.748497683001 27.208429890279927 28.390279562237588 22.652792864481484 78-79 21.773502592053 27.236891360289118 28.3004089773507 22.689197070307184 80-81 21.679152006871963 27.57121277196616 28.229322335055247 22.520312886106634 82-83 21.85742395565546 27.469402629151002 28.13009182428837 22.54308159090516 84-85 21.720732441892192 27.29075636679756 28.085213261149207 22.903297930161045 86-87 21.79179942966304 27.292774812507698 28.168615287310462 22.7468104705188 88-89 21.72904533463555 27.3155097361026 27.99240733291021 22.963037596351636 90-91 21.680732527993822 27.306084174527 28.197253020023165 22.815930277456008 92-93 21.829156630936403 27.07841659159021 28.147235654268325 22.94519112320506 94-95 21.523947366098614 27.01183027243604 28.224385393592264 23.23983696787308 96-97 21.836598562509792 26.841595874478113 28.13482960968015 23.186975953331945 98-99 21.619819795305176 27.008751360103076 27.9673965043834 23.404032340208342 100-101 21.826055235876254 26.721120015062187 28.09977377296102 23.35305097610054 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3613.0 1 2344.0 2 1676.5 3 2128.5 4 1547.5 5 1354.5 6 2501.0 7 5196.5 8 7833.0 9 8331.0 10 6077.5 11 3590.5 12 3020.5 13 2993.0 14 2929.5 15 2886.5 16 2820.5 17 2854.5 18 2960.5 19 3015.5 20 3101.0 21 3350.5 22 3737.5 23 4224.5 24 4917.0 25 5780.5 26 6695.5 27 7777.5 28 8978.0 29 10355.0 30 12549.5 31 14956.5 32 17699.5 33 19899.0 34 21506.5 35 23617.0 36 26682.5 37 29603.5 38 31550.0 39 33059.5 40 34342.0 41 37027.0 42 39572.0 43 42188.5 44 45549.0 45 48666.0 46 49725.0 47 50144.0 48 52055.5 49 52022.0 50 50969.0 51 49126.0 52 47094.0 53 46900.5 54 46956.5 55 45007.5 56 41060.0 57 37399.5 58 34522.0 59 30829.0 60 25961.0 61 21452.5 62 17484.5 63 13407.0 64 10172.5 65 7981.5 66 6137.0 67 4978.5 68 3776.5 69 2564.0 70 1759.0 71 1260.5 72 1040.0 73 854.5 74 631.5 75 464.0 76 321.0 77 183.5 78 92.5 79 68.5 80 41.5 81 14.5 82 8.5 83 6.5 84 5.0 85 2.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.009928419770646148 3 0.007427928865446379 4 0.0 5 2.941754006117377E-4 6 1.4708770030586886E-4 7 0.0 8 3.677192507646722E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001691508553517492 26-27 0.02114385691896865 28-29 4.412631009176066E-4 30-31 7.354385015293443E-5 32-33 0.0034565609571879184 34-35 0.01614287510856911 36-37 0.007795648116211051 38-39 0.02103354114373925 40-41 0.02309276894802141 42-43 0.01853305023853948 44-45 0.009965191695722617 46-47 0.0021327716544350984 48-49 1.4708770030586886E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.7803502599407384E-4 68-69 4.412631009176066E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.677192507646722E-4 82-83 0.0012134735275234182 84-85 0.004559718709481935 86-87 7.722104266058115E-4 88-89 0.0 90-91 0.004265543308870197 92-93 5.883508012234754E-4 94-95 7.354385015293443E-5 96-97 1.4708770030586886E-4 98-99 1.1031577522940165E-4 100-101 0.002721122455658574 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1359733.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.46617582048558 #Duplication Level Percentage of deduplicated Percentage of total 1 75.86802338650111 40.563730775353804 2 13.474285824329002 14.40837069878102 3 4.464427429414969 7.160875856366979 4 1.9991444321031133 4.2754643078947945 5 1.090150764227011 2.914309621549905 6 0.663824639024458 2.129529891843123 7 0.4487396602547834 1.6794675500965048 8 0.3238345503941751 1.3851356006498294 9 0.23186905123269475 1.1157436314482794 >10 1.2909129949313207 12.845026607775972 >50 0.0989818379629746 3.6159470574356622 >100 0.04319192316178506 4.141959159622552 >500 0.0012379767454208722 0.412289422616806 >1k 9.628708019940116E-4 1.036680645951926 >5k 1.3755297171343022E-4 0.5465813394377634 >10k+ 2.7510594342686044E-4 1.7688878331750657 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13159 0.9677635241624643 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10893 0.8011131597159148 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7432 0.5465778943366087 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3624 0.2665229129542344 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2512 0.1847421515841713 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2445 0.1798147136239247 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1934 0.14223380619577522 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 7.354385015293443E-5 0.0 22-23 0.0 0.0 0.0 3.677192507646722E-4 0.0 24-25 0.0 0.0 0.0 8.089823516822788E-4 0.0 26-27 0.0 0.0 0.0 0.002721122455658574 0.0 28-29 0.0 0.0 0.0 0.007611788490828714 0.0 30-31 0.0 0.0 0.0 0.0220999269709568 0.0 32-33 0.0 0.0 0.0 0.04659002907188396 0.0 34-35 0.0 0.0 0.0 0.08192784907036896 0.0 36-37 0.0 0.0 0.0 0.13896110486396962 0.0 38-39 0.0 0.0 0.0 0.21827814725390943 0.0 40-41 0.0 0.0 0.0 0.30494957465914263 0.0 42-43 0.0 0.0 0.0 0.3893779146347114 0.0 44-45 0.0 0.0 0.0 0.493552778376343 0.0 46-47 0.0 0.0 0.0 0.6139072891516202 0.0 48-49 0.0 0.0 0.0 0.7412852376165027 0.0 50-51 0.0 0.0 0.0 0.8737377117419376 0.0 52-53 0.0 0.0 0.0 0.9959675906961146 0.0 54-55 0.0 0.0 0.0 1.1259931177665026 0.0 56-57 0.0 0.0 0.0 1.2825679747421002 0.0 58-59 0.0 0.0 0.0 1.449034479563267 0.0 60-61 0.0 0.0 0.0 1.6207225977452926 0.0 62-63 0.0 0.0 0.0 1.7911972423997946 0.0 64-65 0.0 0.0 0.0 1.964687184910567 0.0 66-67 0.0 0.0 0.0 2.162483369896884 0.0 68-69 0.0 0.0 0.0 2.3790700085972762 0.0 70-71 0.0 0.0 0.0 2.603930330439873 0.0 72-73 0.0 0.0 0.0 2.838167493176969 0.0 74-75 0.0 0.0 0.0 3.0634323061954074 0.0 76-77 0.0 0.0 0.0 3.30447227507165 0.0 78-79 0.0 0.0 0.0 3.567428311293467 0.0 80-81 0.0 0.0 0.0 3.854359642665141 0.0 82-83 0.0 0.0 0.0 4.14831441172642 0.0 84-85 0.0 0.0 0.0 4.435650234273934 0.0 86-87 0.0 0.0 0.0 4.728943108683837 0.0 88-89 0.0 0.0 0.0 5.0478292429469604 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4775 0.0 53.222946 1 GTATCAA 8005 0.0 38.927902 1 ATCAACG 10700 0.0 28.901188 3 TATCAAC 10785 0.0 28.806604 2 TCAACGC 10900 0.0 28.543116 4 CAACGCA 11035 0.0 28.107836 5 AACGCAG 11285 0.0 27.442053 6 TACCGTC 135 1.3435156E-5 24.628323 7 GTACATG 8585 0.0 24.235518 1 TGGTATC 2220 0.0 24.180202 2 ACGCAGA 12825 0.0 24.035763 7 GTGGTAT 2265 0.0 23.908653 1 CGCAGAG 12945 0.0 23.77626 8 TACATGG 8735 0.0 23.330757 2 ATACCGT 190 3.2895332E-7 22.498806 6 GAGTACT 8145 0.0 22.479565 12-13 ACATGGG 8745 0.0 22.108185 3 GCAGAGT 13755 0.0 22.06454 9 GTACTTT 8465 0.0 21.657831 14-15 AGTACTT 8320 0.0 21.207531 12-13 >>END_MODULE