FastQCFastQC Report
Thu 26 May 2016
SRR938217_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938217_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381371
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138181.000310561029586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110310.7985544795713824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74090.5363512047089449No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36270.26256523410437893No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25520.18474399708695202No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24110.17453674646420114No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20520.14854807289279998No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46650.056.483441
GTATCAA84200.042.2355271
TATCAAC108100.032.7961042
ATCAACG108300.032.735543
TCAACGC110150.032.2720264
CAACGCA111300.031.853185
GTGGTAT19500.031.82311
AACGCAG115400.030.721486
TGGTATC20200.029.8786852
ACGCAGA127350.027.6228087
CGCAGAG129750.027.0742478
GTACATG85600.025.8748571
TACATGG85900.025.2832072
GAGTACT81600.025.1040312-13
GCAGAGT135850.025.086929
TAAGGTG4750.025.012415
ACATGGG85150.024.2222353
GTACTTT85700.023.78046614-15
CAGAGTA133400.022.39347610-11
CATGGGG36450.022.2950124
Produced by FastQC (version 0.11.5)