##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938217_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1381371 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.981490128285593 33.0 31.0 34.0 30.0 34.0 2 32.13853917593463 34.0 31.0 34.0 30.0 34.0 3 32.19976385779056 34.0 31.0 34.0 30.0 34.0 4 35.72674972907351 37.0 35.0 37.0 35.0 37.0 5 35.755168596995304 37.0 35.0 37.0 35.0 37.0 6 35.79808393255686 37.0 36.0 37.0 35.0 37.0 7 35.74905872499133 37.0 36.0 37.0 35.0 37.0 8 35.734918425245645 37.0 36.0 37.0 35.0 37.0 9 37.39925552223117 39.0 38.0 39.0 35.0 39.0 10-11 37.320376640308794 39.0 37.0 39.0 34.5 39.0 12-13 37.22212099428756 39.0 37.0 39.0 34.0 39.0 14-15 38.46125190119092 40.0 38.0 41.0 34.0 41.0 16-17 38.39383807825703 40.0 38.0 41.0 34.0 41.0 18-19 38.36764308791773 40.0 38.0 41.0 34.0 41.0 20-21 38.28888039491201 40.0 38.0 41.0 34.0 41.0 22-23 38.13114145294783 40.0 38.0 41.0 33.5 41.0 24-25 38.06903576229702 40.0 38.0 41.0 33.5 41.0 26-27 38.07087560112381 40.0 38.0 41.0 33.0 41.0 28-29 37.96794018406351 40.0 38.0 41.0 33.0 41.0 30-31 37.751063979191684 40.0 37.5 41.0 33.0 41.0 32-33 37.49443849624757 40.0 36.5 41.0 33.0 41.0 34-35 37.27823806928045 40.0 36.0 41.0 32.0 41.0 36-37 37.06368383294568 40.0 36.0 41.0 31.5 41.0 38-39 36.83317443322612 39.5 35.0 41.0 31.0 41.0 40-41 36.596720938835404 39.0 35.0 41.0 30.5 41.0 42-43 36.2902999990589 39.0 35.0 41.0 30.0 41.0 44-45 35.9968853407231 39.0 35.0 41.0 29.5 41.0 46-47 35.757608926204476 39.0 35.0 41.0 28.0 41.0 48-49 35.4596154834581 39.0 35.0 41.0 26.5 41.0 50-51 34.798091171741696 38.5 34.5 40.0 25.5 40.5 52-53 34.87223417894251 38.5 35.0 40.0 26.0 41.0 54-55 35.00235273507262 39.0 35.0 40.0 26.0 41.0 56-57 34.80019198318193 38.0 35.0 40.0 25.5 41.0 58-59 34.547044204634375 38.0 34.5 40.0 25.0 41.0 60-61 34.18543316748361 37.5 34.0 40.0 23.5 41.0 62-63 33.779443393556114 37.0 34.0 40.0 22.5 41.0 64-65 33.36552164480071 36.5 33.0 39.5 21.5 41.0 66-67 32.94169741510427 36.0 33.0 39.0 20.0 41.0 68-69 32.47218451813452 35.0 32.5 39.0 18.0 40.5 70-71 32.01010807379046 35.0 32.0 38.0 14.5 40.0 72-73 31.537245967955023 35.0 31.5 37.0 10.0 39.5 74-75 31.029036370388546 35.0 31.0 37.0 7.0 39.0 76-77 30.517881510470396 35.0 30.5 36.0 3.5 39.0 78-79 29.973442688459507 34.0 30.0 36.0 2.0 37.5 80-81 29.463313621033016 34.0 29.5 35.0 2.0 37.0 82-83 28.955235414671368 34.0 29.0 35.0 2.0 37.0 84-85 28.482946290315926 34.0 29.0 35.0 2.0 36.0 86-87 27.994309276798194 34.0 27.5 35.0 2.0 36.0 88-89 27.466866612951915 33.5 26.5 35.0 2.0 35.0 90-91 26.89394485623341 33.0 25.5 35.0 2.0 35.0 92-93 26.289071147432516 33.0 24.5 35.0 2.0 35.0 94-95 25.72405385663953 32.0 23.0 35.0 2.0 35.0 96-97 24.920633920937966 32.0 18.0 34.5 2.0 35.0 98-99 23.888348604393748 31.5 2.0 34.0 2.0 35.0 100-101 21.07316861292151 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35702240308597055 1101 2 0.3184648969872086 1101 3 0.35819230851475936 1101 4 0.28129737900774643 1101 5 0.3093164543890481 1101 6 0.2496838923541702 1101 7 0.3046693037563415 1101 8 0.23602485608751778 1101 9 0.33066008447194406 1101 10-11 0.4387230160228839 1101 12-13 0.31870866990466595 1101 14-15 0.39518320584576827 1101 16-17 0.4280957257628373 1101 18-19 0.5000348006993391 1101 20-21 0.49884945457410623 1101 22-23 0.4705082428334961 1101 24-25 0.34433407146966744 1101 26-27 0.4068733055335585 1101 28-29 0.47157819791742384 1101 30-31 0.4573212480681903 1101 32-33 0.3859284922072632 1101 34-35 0.3735104057832572 1101 36-37 0.4216779024456372 1101 38-39 0.34665627071125016 1101 40-41 0.3502737076399214 1101 42-43 0.32388340857522735 1101 44-45 0.3236809573483015 1101 46-47 0.39836598765510445 1101 48-49 0.46378434126165047 1101 50-51 0.45700600522540924 1101 52-53 0.49315854222783173 1101 54-55 0.43365429768017094 1101 56-57 0.2169254097685993 1101 58-59 0.3124307188028652 1101 60-61 0.3966036259014558 1101 62-63 0.534277158422519 1101 64-65 0.45076017927465273 1101 66-67 0.28793327918079115 1101 68-69 0.3546120599265947 1101 70-71 0.48244484274736266 1101 72-73 0.45441646050225515 1101 74-75 0.4089658185096461 1101 76-77 0.5338866230369774 1101 78-79 0.44049193180294566 1101 80-81 0.5460469861594532 1101 82-83 0.579415945416045 1101 84-85 0.7319466472672751 1101 86-87 0.8459561748171822 1101 88-89 0.7027387785610237 1101 90-91 0.7786850997276105 1101 92-93 0.9885453747651063 1101 94-95 1.097472556743611 1101 96-97 1.006780536692446 1101 98-99 1.4573765689406457 1101 100-101 1.2493878520012451 1102 1 0.3454736816283699 1102 2 0.2484704216958349 1102 3 0.13727446717329883 1102 4 0.13254980040201048 1102 5 0.09168338750539817 1102 6 0.2990705110013323 1102 7 0.22211305112690383 1102 8 0.24902179091444765 1102 9 0.24991843279210713 1102 10-11 0.37515217030326653 1102 12-13 0.2154622101102035 1102 14-15 0.3942689705902751 1102 16-17 0.2299384125742776 1102 18-19 0.15643924404449194 1102 20-21 0.26262048429656915 1102 22-23 0.20051064689079823 1102 24-25 0.2176922621391455 1102 26-27 0.2412120857518616 1102 28-29 0.28084023322932694 1102 30-31 0.17035800009430346 1102 32-33 0.2480979921171098 1102 34-35 0.1718386035262185 1102 36-37 0.3225489686912866 1102 38-39 0.3340976718143409 1102 40-41 0.3664331914148491 1102 42-43 0.5364537965993179 1102 44-45 0.27895017709300873 1102 46-47 0.43872612237477426 1102 48-49 0.32103361426546684 1102 50-51 0.2949089737738646 1102 52-53 0.44625601981692853 1102 54-55 0.4405934126172255 1102 56-57 0.4859778528918355 1102 58-59 0.6512765285772062 1102 60-61 0.5761913300729518 1102 62-63 0.6467939752657159 1102 64-65 0.5981988180234339 1102 66-67 0.5693198076758108 1102 68-69 0.4082366964285029 1102 70-71 0.5152587802696118 1102 72-73 0.8330104105995666 1102 74-75 0.7216521907409366 1102 76-77 0.5914562829091032 1102 78-79 0.6217803909409092 1102 80-81 0.8910959572691581 1102 82-83 0.8938498823835097 1102 84-85 0.9096761191606113 1102 86-87 0.8092934340069213 1102 88-89 0.8121736632818539 1102 90-91 1.0351477374407985 1102 92-93 0.9467066870105398 1102 94-95 1.0682811752890622 1102 96-97 1.2063814400632111 1102 98-99 1.1109553781001011 1102 100-101 0.9655709718203873 1103 1 0.40408176929290107 1103 2 0.42274899680731437 1103 3 0.29061769713969454 1103 4 0.256155536671848 1103 5 0.21455424210988383 1103 6 0.22889072846285075 1103 7 0.27327486674356294 1103 8 0.27232496218096713 1103 9 0.303306424247765 1103 10-11 0.43106295328936284 1103 12-13 0.27231411757291824 1103 14-15 0.5295067447543929 1103 16-17 0.36193607817761375 1103 18-19 0.5491935487965804 1103 20-21 0.43840905141897224 1103 22-23 0.5166358317444164 1103 24-25 0.5996987100600037 1103 26-27 0.39009843123123034 1103 28-29 0.4839894023087652 1103 30-31 0.5505606733184294 1103 32-33 0.5129004466332034 1103 34-35 0.5286565149211313 1103 36-37 0.49720433355408034 1103 38-39 0.6039692233382112 1103 40-41 0.7322787586109101 1103 42-43 0.59019931336789 1103 44-45 0.803844364332015 1103 46-47 0.8529101317576888 1103 48-49 1.0252689577107432 1103 50-51 0.8375332435666607 1103 52-53 0.9244520751398682 1103 54-55 0.9147564010982236 1103 56-57 0.8575055795181257 1103 58-59 1.0045803797650876 1103 60-61 0.8966243833164569 1103 62-63 0.9574401271941468 1103 64-65 1.1399536383825435 1103 66-67 1.09595544348727 1103 68-69 1.0580987290533983 1103 70-71 1.1742116447216233 1103 72-73 1.1295144816286857 1103 74-75 1.2063769075820225 1103 76-77 1.1201163845573454 1103 78-79 1.1290442174249762 1103 80-81 1.4135160806748814 1103 82-83 1.2080069782127616 1103 84-85 1.1880441379264397 1103 86-87 1.1735652665649532 1103 88-89 1.314089625002282 1103 90-91 1.3604266468469461 1103 92-93 1.2427291470793307 1103 94-95 1.0953587599817673 1103 96-97 1.1277720492160874 1103 98-99 1.1739432924980022 1103 100-101 0.7552149196704185 1104 1 0.2125447620348666 1104 2 0.11192388489457983 1104 3 0.08668445362629029 1104 4 0.11702736943293957 1104 5 0.19785860127368693 1104 6 0.16146711161727012 1104 7 0.030537242335718418 1104 8 0.08563388477647749 1104 9 -0.055634642291416014 1104 10-11 0.13661567179082112 1104 12-13 0.06347355518428799 1104 14-15 0.08210029510425443 1104 16-17 0.10933220577985736 1104 18-19 0.20080433153377442 1104 20-21 0.13052300682772966 1104 22-23 0.15533970848830592 1104 24-25 0.22694294057850328 1104 26-27 0.3057770787170071 1104 28-29 0.35676472935470827 1104 30-31 0.2698677922718815 1104 32-33 0.4114347158322147 1104 34-35 0.4101251793567897 1104 36-37 0.42662488638124074 1104 38-39 0.40935352947929005 1104 40-41 0.32482732656257696 1104 42-43 0.1702321266148772 1104 44-45 0.29164967964341315 1104 46-47 0.47010612028391563 1104 48-49 0.24482489234501514 1104 50-51 0.2837928593691714 1104 52-53 0.13376636520027319 1104 54-55 0.3998410418909586 1104 56-57 0.3187131504686178 1104 58-59 0.3709212681131291 1104 60-61 0.3903844337315192 1104 62-63 0.5955138157507918 1104 64-65 0.44765279958033943 1104 66-67 0.41547769564945725 1104 68-69 0.5227696187052757 1104 70-71 0.4850845640140733 1104 72-73 0.5112314184794862 1104 74-75 0.34845392092462646 1104 76-77 0.4008788036107589 1104 78-79 0.3573706331753357 1104 80-81 0.6115125168520308 1104 82-83 0.5721373185143825 1104 84-85 0.7184621265395918 1104 86-87 0.6659763882999279 1104 88-89 0.5209282533650921 1104 90-91 0.5295827379417339 1104 92-93 0.5554404774283199 1104 94-95 0.621569155913793 1104 96-97 0.5171044843501633 1104 98-99 0.7173170810155405 1104 100-101 0.8624658866833279 1105 1 0.17603203121379352 1105 2 0.23157148220535362 1105 3 0.27180026729899964 1105 4 0.12422592724054482 1105 5 0.24826297746758996 1105 6 0.20320096757995287 1105 7 0.23217748810382943 1105 8 0.26249781921626436 1105 9 0.3170209681181646 1105 10-11 0.34824593219585864 1105 12-13 0.2452728018372241 1105 14-15 0.2692100803465962 1105 16-17 0.31328117912742215 1105 18-19 0.23316528070144216 1105 20-21 0.3643644979752807 1105 22-23 0.36114517376901034 1105 24-25 0.37860520376207063 1105 26-27 0.2900527160850004 1105 28-29 0.29857414743338495 1105 30-31 0.37601809369339634 1105 32-33 0.48433923674151913 1105 34-35 0.4312127983335259 1105 36-37 0.4641097946316677 1105 38-39 0.5154211363826278 1105 40-41 0.5500995982636923 1105 42-43 0.5034755296596884 1105 44-45 0.3586492951139064 1105 46-47 0.2376998958675287 1105 48-49 0.25878837858051185 1105 50-51 0.34939390380093727 1105 52-53 0.2916431214251105 1105 54-55 0.3635097221691339 1105 56-57 0.3961741393296663 1105 58-59 0.3076863205827962 1105 60-61 0.20816396187660047 1105 62-63 0.20528613706861165 1105 64-65 0.2539946421795918 1105 66-67 0.1778565481803298 1105 68-69 0.402149843965141 1105 70-71 0.2776381293882224 1105 72-73 0.4941643036085246 1105 74-75 0.34052558399408284 1105 76-77 0.1754747375212986 1105 78-79 0.23370770016616405 1105 80-81 0.257722627083826 1105 82-83 0.4190894053105474 1105 84-85 0.3130600037713087 1105 86-87 0.27452689811163467 1105 88-89 0.27960911041108005 1105 90-91 0.012517173592993203 1105 92-93 0.32961105141097136 1105 94-95 0.5880561687397154 1105 96-97 0.5007102961209036 1105 98-99 0.4340237511553795 1105 100-101 0.3851174657351528 1106 1 0.13388937498789488 1106 2 0.07011158933514139 1106 3 0.10605004121341466 1106 4 0.20311543112256203 1106 5 0.01897438337213231 1106 6 0.13177001971423152 1106 7 0.051842851186698624 1106 8 0.16289917784698105 1106 9 0.19625496897108974 1106 10-11 -0.3010885141742179 1106 12-13 -0.08442047790097718 1106 14-15 -0.02522972644474919 1106 16-17 -0.028756827447971034 1106 18-19 0.0407278814569878 1106 20-21 0.0891389416837498 1106 22-23 -0.03261652748346222 1106 24-25 0.013630473930568598 1106 26-27 0.07483511437673229 1106 28-29 0.005019779520296197 1106 30-31 0.1412527841451805 1106 32-33 0.020526541468598225 1106 34-35 -0.18857021999225765 1106 36-37 -0.18489043825917406 1106 38-39 -0.0161529637362392 1106 40-41 0.14852471176016735 1106 42-43 -0.06906618653408714 1106 44-45 0.044407717747262154 1106 46-47 0.13465174795462787 1106 48-49 0.10004151277705375 1106 50-51 0.203296488889265 1106 52-53 0.20654949628257668 1106 54-55 -0.054336521483072886 1106 56-57 0.04191692500658206 1106 58-59 -0.0358836237956055 1106 60-61 -0.30349192224660015 1106 62-63 -0.025237232166205104 1106 64-65 0.009432038399502574 1106 66-67 -0.18317340400143678 1106 68-69 -0.4538449779943434 1106 70-71 -0.1904089448071673 1106 72-73 0.0967496943963333 1106 74-75 0.01761562806186845 1106 76-77 0.203354342030309 1106 78-79 0.31529737675035463 1106 80-81 0.17458815833202834 1106 82-83 0.04577899811452468 1106 84-85 0.29271834988339407 1106 86-87 0.38981996250141293 1106 88-89 0.2014001317130294 1106 90-91 0.16637333174099922 1106 92-93 0.29890499897121714 1106 94-95 -0.15847164054386198 1106 96-97 0.060235077823545424 1106 98-99 0.1941597957010437 1106 100-101 -0.06289192248783237 1107 1 0.27460006367842027 1107 2 0.20519735267334482 1107 3 0.20271681732368307 1107 4 0.1861007575904523 1107 5 0.11225697294101167 1107 6 0.17387963557194297 1107 7 0.12577343253684603 1107 8 0.17451257165028977 1107 9 0.16730047419369498 1107 10-11 0.2351896610107289 1107 12-13 0.068468666673553 1107 14-15 0.2459234617663668 1107 16-17 0.14773311635035924 1107 18-19 0.2823534235002185 1107 20-21 0.3863014021472466 1107 22-23 0.4097747391073554 1107 24-25 0.4156577501314871 1107 26-27 0.376683902336417 1107 28-29 0.3360985746318761 1107 30-31 0.34706552423708814 1107 32-33 0.3563218800276289 1107 34-35 0.29842978333863357 1107 36-37 0.29045620056123767 1107 38-39 0.21505449898513262 1107 40-41 0.2542317673989132 1107 42-43 0.2401802752256046 1107 44-45 0.12451115622735642 1107 46-47 0.13750370549622204 1107 48-49 0.19341332267446631 1107 50-51 -0.030816225649928697 1107 52-53 -0.05563098192887139 1107 54-55 0.05033081992719701 1107 56-57 0.14157293900394308 1107 58-59 -0.19112842296421917 1107 60-61 -0.15088542767286128 1107 62-63 -0.09683761645599986 1107 64-65 -0.13323888038906517 1107 66-67 -0.16385351510096768 1107 68-69 -0.008544414930803157 1107 70-71 0.11873069047916118 1107 72-73 -0.024864037254612725 1107 74-75 -0.11689912517373813 1107 76-77 -0.004500948782755643 1107 78-79 0.02967526838120449 1107 80-81 0.212745670517144 1107 82-83 -0.028577223682930253 1107 84-85 -0.13031091581189003 1107 86-87 0.19094023673853044 1107 88-89 0.37000109510224277 1107 90-91 0.2601367594004387 1107 92-93 0.42235833483602647 1107 94-95 0.4706063827524041 1107 96-97 0.417212987821884 1107 98-99 0.1669276101455992 1107 100-101 0.2828956170248773 1108 1 0.3074281856482557 1108 2 0.15208386437455346 1108 3 0.1285243085319081 1108 4 0.150519854174199 1108 5 0.22830034741507887 1108 6 0.2035038860948717 1108 7 0.11139847765763733 1108 8 0.10560102445405306 1108 9 0.17435984396056625 1108 10-11 0.23249656877461433 1108 12-13 0.22903217056703795 1108 14-15 0.2780539513452922 1108 16-17 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-0.9483809878483171 2316 60-61 -0.8813528487663049 2316 62-63 -0.9647206135323501 2316 64-65 -0.883783934449788 2316 66-67 -1.070318136883099 2316 68-69 -0.8938708688544139 2316 70-71 -1.052722378315508 2316 72-73 -1.1128446130389271 2316 74-75 -1.0407992168723368 2316 76-77 -1.0100035123716786 2316 78-79 -0.9640826214112437 2316 80-81 -0.9524575170021734 2316 82-83 -1.0812229700594536 2316 84-85 -1.0838483490454642 2316 86-87 -1.173416638325147 2316 88-89 -1.2592393175587269 2316 90-91 -1.120827319270397 2316 92-93 -1.239303620261019 2316 94-95 -1.2609759707068484 2316 96-97 -1.177882432256137 2316 98-99 -0.9755918304756719 2316 100-101 -0.9373129152621829 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3810.0 3 954.0 4 892.0 5 1111.0 6 1151.0 7 1513.0 8 1848.0 9 2197.0 10 2845.0 11 3669.0 12 4880.0 13 6129.0 14 7186.0 15 8905.0 16 11665.0 17 16913.0 18 14739.0 19 10352.0 20 9898.0 21 10036.0 22 10503.0 23 11718.0 24 12926.0 25 14546.0 26 16635.0 27 19359.0 28 22756.0 29 26868.0 30 32393.0 31 39509.0 32 49906.0 33 65714.0 34 92157.0 35 144678.0 36 201681.0 37 272762.0 38 204466.0 39 22100.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.11095655008141 18.449445690148142 14.419913539594702 22.019684220175744 2 15.796099086590312 20.112468683251596 40.0900734508576 24.00135877930049 3 19.170745381701813 25.357378013401995 29.4228856658368 26.048990939059397 4 12.742835989675507 16.884368160984405 36.147237734957876 34.22555811438221 5 13.655508001475166 37.63385000394875 33.140822630042535 15.569819364533544 6 31.627436400295117 35.685554883961615 17.251315768494322 15.435692947248953 7 28.722899456594575 33.710841086694934 19.74631421018583 17.81994524652466 8 27.32837553881067 34.057614623344996 19.09608014561922 19.51792969222512 9 27.685491277730616 16.421870427725835 19.803071699053458 36.08956659549009 10-11 25.62670754758674 26.83545743619541 25.803883156109425 21.733951860108427 12-13 26.70818887046464 24.491130752907615 26.9023078305639 21.898372546063847 14-15 23.492136270361925 25.350559439191816 26.06511470911842 25.092189581327833 16-17 21.975734597431046 28.825216373387885 26.60164840737017 22.597400621810902 18-19 21.91569759966447 26.579787170466812 30.08802280632049 21.416492423548227 20-21 22.873092290917256 25.50651872298959 30.384240593223716 21.236148392869435 22-23 22.577269625463444 25.539543590167092 30.429048713947726 21.454138070421738 24-25 22.04716821174394 25.863463185520864 30.52220810067808 21.567160502057124 26-27 21.73384995043069 26.23663376414138 30.36162593121294 21.667890354214986 28-29 21.625139046201916 26.28531936526473 30.581942086509827 21.507599502023528 30-31 21.73625303642698 26.042020599526346 30.471418646215415 21.75030771783126 32-33 21.455358569150125 26.349599170920797 30.27664107006567 21.918401189863413 34-35 22.016674672190113 26.26902036300883 30.14130292248697 21.573002042314084 36-37 22.00872517790481 25.992974362726596 30.02271849710187 21.975581962266723 38-39 21.690673103629084 26.27552415291262 30.224102325106433 21.80970041835186 40-41 21.854808979958992 26.258239366590885 30.2360943827093 21.650857270740826 42-43 21.911591578281058 26.153738630639594 29.87069610161801 22.063973689461335 44-45 21.71255948398458 26.442821517634485 29.818601000402595 22.026017997978343 46-47 21.924536759282827 26.646284569939326 29.38515899013035 22.044019680647494 48-49 22.048505067350447 26.754147602173433 29.062669607157687 22.13467772331843 50-51 22.013822837552148 27.02278208411373 28.834943125348712 22.128451952985408 52-53 22.20683058160488 26.954073573088106 28.700984554090375 22.13811129121664 54-55 21.910355224970022 26.924207666417573 28.820898721491346 22.34453838712107 56-57 21.95568341822982 27.23249279233712 28.635538638612505 22.176285150820547 58-59 22.124751183349904 26.803952392296516 28.648761763805027 22.42253466054856 60-61 22.004764887558068 26.930847577909173 28.895893000032586 22.168494534500173 62-63 21.974803393627642 26.98755085771137 28.950718253166592 22.0869274954944 64-65 21.87824260965587 26.83836281023153 28.874350572944007 22.40904400716859 66-67 21.90730849405446 27.07775361862374 28.733440058387437 22.281497828934363 68-69 21.91192985784324 26.89653973952288 28.799722825815433 22.391807576818447 70-71 21.823439095577104 27.00525793578945 28.649992395547415 22.52131057308603 72-73 21.855212287405713 27.085478030037763 28.450021128619518 22.609288553937002 74-75 21.718413209806407 27.328612545246315 28.389866562967196 22.563107681980078 76-77 21.864276564793354 27.084152468919914 28.49255880940951 22.559012156877227 78-79 21.8291773462502 27.105905266663772 28.42546736753696 22.639450019549066 80-81 21.75014553532381 27.444884831598976 28.330984977279112 22.4739846557981 82-83 21.953933100898965 27.313355563037867 28.173398936305016 22.559312399758152 84-85 21.770029277716173 27.223098948688513 28.158776671521547 22.848095102073767 86-87 21.96940847011943 27.090271583629686 28.231571524551995 22.70874842169889 88-89 21.88219821063415 27.116819365447576 28.110251646094188 22.89073077782409 90-91 21.85649463836827 27.1389576896653 28.192705109711635 22.811842562254796 92-93 22.051352797746944 26.869153633301686 28.296251880143007 22.78324168880836 94-95 21.835261497275074 26.73675998922198 28.359546801797496 23.068431711705447 96-97 22.025396397747016 26.614615132243635 28.248332243343636 23.111656226665712 98-99 21.93862070014317 26.63664494394553 28.070503856573552 23.354230499337746 100-101 22.257312214556407 26.176038474424107 28.332755617500876 23.23389369351861 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3784.0 1 2443.5 2 1746.0 3 2197.5 4 1573.0 5 1354.0 6 2528.5 7 5325.0 8 8110.0 9 8543.0 10 6145.5 11 3738.0 12 3181.5 13 3141.0 14 3106.5 15 2985.5 16 2869.0 17 2901.5 18 2940.5 19 3018.5 20 3242.0 21 3544.5 22 3896.5 23 4473.0 24 5216.0 25 6054.0 26 6946.0 27 8051.0 28 9172.0 29 10528.0 30 12627.0 31 14920.0 32 17668.0 33 20068.0 34 21816.0 35 23820.0 36 26920.0 37 29796.5 38 31787.0 39 33470.5 40 34843.5 41 37435.5 42 39741.0 43 42378.5 44 45887.5 45 49138.0 46 50352.0 47 50655.5 48 52713.5 49 52650.5 50 51255.5 51 49690.5 52 47800.5 53 47443.0 54 47269.0 55 45081.0 56 41505.5 57 38452.0 58 35292.0 59 31236.5 60 26512.0 61 21975.0 62 17897.0 63 13880.0 64 10755.5 65 8545.5 66 6684.0 67 5463.5 68 4196.0 69 2823.0 70 2035.5 71 1529.5 72 1182.5 73 969.5 74 743.5 75 568.5 76 388.0 77 222.0 78 128.0 79 94.5 80 66.0 81 40.0 82 26.5 83 20.0 84 14.0 85 10.5 86 4.5 87 2.5 88 5.0 89 4.0 90 2.5 91 1.5 92 0.0 93 1.5 94 1.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.49725960657926077 2 0.05212213083957894 3 0.06008523416229239 4 0.06978574184632513 5 0.08600151588530526 6 0.1135828101212491 7 0.0725366320850807 8 0.05675520913643041 9 0.05552454771382923 10-11 0.0797758169239111 12-13 0.11199018945670643 14-15 0.05733434392353684 16-17 0.06421156952042571 18-19 0.06345145511234852 20-21 0.08078930280134736 22-23 0.06841029672694736 24-25 0.09331309257252396 26-27 0.07098020734473215 28-29 0.07126977473828537 30-31 0.07586665711094268 32-33 0.07561328564158361 34-35 0.07774884516903859 36-37 0.07224706469152747 38-39 0.04310934571523508 40-41 0.04719948514917426 42-43 0.045715452257214025 44-45 0.023672134422975437 46-47 0.03044077224728187 48-49 0.030585555944058474 50-51 0.02863097603757427 52-53 0.028269016795632745 54-55 0.02801564532627368 56-57 0.027183139069808186 58-59 0.024178877361693565 60-61 0.03156284589730058 62-63 0.022586256697150874 64-65 0.02504757954235321 66-67 0.01824274579385263 68-69 0.020993636032608183 70-71 0.04350750088137076 72-73 0.039272577750654965 74-75 0.03311927063764911 76-77 0.024758012148799996 78-79 0.016722516977698245 80-81 0.019039056126123974 82-83 0.024323661058470173 84-85 0.02074026456324912 86-87 0.010062466925974269 88-89 0.04361608865395321 90-91 0.056067486576741515 92-93 0.03561678940704561 94-95 0.056682817288042094 96-97 0.04517251339430175 98-99 0.03489287092316257 100-101 0.1720754236189988 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1381371.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.756116944051726 #Duplication Level Percentage of deduplicated Percentage of total 1 77.03707014551323 42.18250821914838 2 12.882502382378172 14.107916139630483 3 4.22653572268922 6.942860528993493 4 1.881132307010622 4.1201400235962975 5 1.047888039069995 2.8689140005794838 6 0.6228498373142198 2.046290311833661 7 0.41337551122025973 1.5844386483928596 8 0.2966989053714763 1.2996863967754155 9 0.2358531265946498 1.1622961243293195 >10 1.221804787386226 12.504373195178504 >50 0.0900883483544726 3.3643860841821978 >100 0.04155415114859798 3.9903156974957907 >500 0.0013233679744514464 0.43513726187230284 >1k 9.263575821160125E-4 1.0532423149565233 >5k 1.3233679744514463E-4 0.5368746000352859 >10k+ 2.6467359489028926E-4 1.8006204529999756 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13818 1.000310561029586 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11031 0.7985544795713824 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7409 0.5363512047089449 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3627 0.26256523410437893 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2552 0.18474399708695202 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2411 0.17453674646420114 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2052 0.14854807289279998 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.239184838830409E-5 0.0 14-15 0.0 0.0 0.0 7.239184838830409E-5 0.0 16-17 0.0 0.0 0.0 7.239184838830409E-5 0.0 18-19 0.0 0.0 0.0 7.239184838830409E-5 0.0 20-21 0.0 0.0 0.0 1.8097962097076022E-4 0.0 22-23 0.0 0.0 0.0 7.239184838830408E-4 0.0 24-25 0.0 0.0 0.0 0.0015202288161543856 0.0 26-27 0.0 0.0 0.0 0.0034024168742502916 0.0 28-29 0.0 0.0 0.0 0.009338548442091227 0.0 30-31 0.0 0.0 0.0 0.022731040393927482 0.0 32-33 0.0 0.0 0.0 0.04553447263624327 0.0 34-35 0.0 0.0 0.0 0.07995679654488186 0.0 36-37 0.0 0.0 0.0 0.1350831890925754 0.0 38-39 0.0 0.0 0.0 0.21598107966650523 0.0 40-41 0.0 0.0 0.0 0.30274270995988767 0.0 42-43 0.0 0.0 0.0 0.38729638887742684 0.0 44-45 0.0 0.0 0.0 0.48408429017258947 0.0 46-47 0.0 0.0 0.0 0.6030602929987672 0.0 48-49 0.0 0.0 0.0 0.7263798067282432 0.0 50-51 0.0 0.0 0.0 0.8586759096578689 0.0 52-53 0.0 0.0 0.0 0.9868818731535554 0.0 54-55 0.0 0.0 0.0 1.1209153804445005 0.0 56-57 0.0 0.0 0.0 1.2775351444325964 0.0 58-59 0.0 0.0 0.0 1.4490676291886828 0.0 60-61 0.0 0.0 0.0 1.6305901890223553 0.0 62-63 0.0 0.0 0.0 1.806176617288187 0.0 64-65 0.0 0.0 0.0 1.981147714842718 0.0 66-67 0.0 0.0 0.0 2.1726965456781704 0.0 68-69 0.0 0.0 0.0 2.3845150940623485 0.0 70-71 0.0 0.0 0.0 2.615408894496844 0.0 72-73 0.0 0.0 0.0 2.8414162451651293 0.0 74-75 0.0 0.0 0.0 3.061740835734933 0.0 76-77 0.0 0.0 0.0 3.298606963661464 0.0 78-79 0.0 0.0 0.0 3.5593262056319412 0.0 80-81 0.0 0.0 0.0 3.834523817280079 0.0 82-83 0.0 0.0 0.0 4.115114621633146 0.0 84-85 0.0 0.0 0.0 4.406491811396069 0.0 86-87 0.0 0.0 0.0 4.702140120213903 0.0 88-89 0.0 0.0 0.0 5.014257574540077 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4665 0.0 56.48344 1 GTATCAA 8420 0.0 42.235527 1 TATCAAC 10810 0.0 32.796104 2 ATCAACG 10830 0.0 32.73554 3 TCAACGC 11015 0.0 32.272026 4 CAACGCA 11130 0.0 31.85318 5 GTGGTAT 1950 0.0 31.8231 1 AACGCAG 11540 0.0 30.72148 6 TGGTATC 2020 0.0 29.878685 2 ACGCAGA 12735 0.0 27.622808 7 CGCAGAG 12975 0.0 27.074247 8 GTACATG 8560 0.0 25.874857 1 TACATGG 8590 0.0 25.283207 2 GAGTACT 8160 0.0 25.10403 12-13 GCAGAGT 13585 0.0 25.08692 9 TAAGGTG 475 0.0 25.01241 5 ACATGGG 8515 0.0 24.222235 3 GTACTTT 8570 0.0 23.780466 14-15 CAGAGTA 13340 0.0 22.393476 10-11 CATGGGG 3645 0.0 22.295012 4 >>END_MODULE