FastQCFastQC Report
Thu 26 May 2016
SRR938217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938217_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381371
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136710.9896689593165052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111910.8101371753135109No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76010.5502504395994993No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35870.25966956016884674No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26020.18836358950636722No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23950.17337847688998828No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19540.14145367175074616No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46500.054.1450461
GTATCAA84550.042.0265581
TATCAAC108150.032.636092
ATCAACG109700.032.0404053
TCAACGC110750.031.7783764
CAACGCA112500.031.2418275
AACGCAG116400.030.154266
GTGGTAT21350.028.0355131
TGGTATC21500.026.5142042
ACGCAGA132150.026.488517
CGCAGAG133050.026.2379368
GTGTAAG4700.025.2684121
GCAGAGT139950.024.5710019
GTACATG82750.024.3407341
TACATGG84100.023.4416352
GAGTACT82750.023.41797412-13
GTACTTT86200.022.37051414-15
ACATGGG86600.022.1582983
TAAGGTA3450.022.027144
AGTACTT84650.022.02266712-13
Produced by FastQC (version 0.11.5)