##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938217_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1381371 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50439092756399 34.0 31.0 34.0 31.0 34.0 2 32.712757832617015 34.0 31.0 34.0 31.0 34.0 3 32.756777867784976 34.0 31.0 34.0 31.0 34.0 4 36.23176105477819 37.0 35.0 37.0 35.0 37.0 5 36.20353185349917 37.0 37.0 37.0 35.0 37.0 6 36.24746067493816 37.0 37.0 37.0 35.0 37.0 7 36.208335776558215 37.0 37.0 37.0 35.0 37.0 8 36.206870565546836 37.0 37.0 37.0 35.0 37.0 9 37.90361749305581 39.0 38.0 39.0 35.0 39.0 10-11 37.89150525094272 39.0 38.0 39.0 35.0 39.0 12-13 37.83418104187796 39.0 38.0 39.0 35.0 39.0 14-15 39.11633912974864 40.5 38.5 41.0 36.0 41.0 16-17 39.08067745739559 40.0 38.0 41.0 36.0 41.0 18-19 39.13371643099501 40.0 38.5 41.0 36.0 41.0 20-21 39.08450879597154 40.0 39.0 41.0 35.5 41.0 22-23 38.92675754739314 40.0 38.0 41.0 35.0 41.0 24-25 38.87854747204046 40.0 38.0 41.0 35.0 41.0 26-27 38.833417669836706 40.0 38.0 41.0 35.0 41.0 28-29 38.75860395216057 40.0 38.0 41.0 35.0 41.0 30-31 38.52658083889122 40.0 38.0 41.0 34.5 41.0 32-33 38.31167875972494 40.0 38.0 41.0 34.0 41.0 34-35 38.094602753351566 40.0 37.0 41.0 34.0 41.0 36-37 37.96481032249845 40.0 37.0 41.0 33.5 41.0 38-39 37.945234118857286 40.0 37.0 41.0 33.0 41.0 40-41 37.86048751566379 40.0 37.0 41.0 33.0 41.0 42-43 37.67663755790443 40.0 37.0 41.0 33.0 41.0 44-45 37.41930154896838 40.0 37.0 41.0 33.0 41.0 46-47 37.021191989697186 40.0 36.0 41.0 31.5 41.0 48-49 36.6283536428664 40.0 36.0 41.0 31.0 41.0 50-51 36.32245537223527 39.5 35.0 41.0 30.0 41.0 52-53 35.91643555569068 39.0 35.0 41.0 29.0 41.0 54-55 35.69817666651464 39.0 35.0 41.0 29.0 41.0 56-57 35.46227950347879 38.5 35.0 40.0 28.0 41.0 58-59 35.136356561705725 38.0 35.0 40.0 27.5 41.0 60-61 34.791998673781336 38.0 34.0 40.0 27.0 41.0 62-63 34.44077767667049 37.0 34.0 40.0 26.0 41.0 64-65 34.05914160641855 36.5 34.0 39.5 26.0 41.0 66-67 33.646353875968146 36.0 33.5 39.0 25.5 41.0 68-69 33.20541150784257 35.5 33.0 39.0 24.5 40.0 70-71 32.71926224019471 35.0 32.5 38.0 22.5 40.0 72-73 32.30334935364938 35.0 32.0 37.0 22.0 39.5 74-75 31.872456059957827 35.0 32.0 37.0 20.5 39.0 76-77 30.583527162507394 33.5 30.0 35.5 20.0 38.0 78-79 31.29546913899307 35.0 31.5 36.0 20.0 38.0 80-81 31.13943176742526 35.0 32.0 35.5 19.5 37.0 82-83 30.790393022584084 35.0 31.0 35.0 18.0 37.0 84-85 30.431485097052132 34.0 31.0 35.0 14.5 36.5 86-87 30.015539996134272 34.0 31.0 35.0 7.5 36.0 88-89 29.580387890002033 34.0 30.0 35.0 4.5 35.5 90-91 29.258850808363576 34.0 30.0 35.0 2.0 35.0 92-93 28.874419326886112 34.0 29.0 35.0 2.0 35.0 94-95 28.500828886664046 34.0 29.0 35.0 2.0 35.0 96-97 27.95134688653519 34.0 29.0 35.0 2.0 35.0 98-99 27.293070435096727 33.5 27.0 35.0 2.0 35.0 100-101 24.95243204034253 31.0 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08838094264355334 1101 2 0.06267143199309544 1101 3 0.11692557363611655 1101 4 -0.09999165644306629 1101 5 0.0251195958627477 1101 6 0.09799489879608814 1101 7 0.12426423201178238 1101 8 0.12591240618274213 1101 9 0.08694927034328259 1101 10-11 0.01699041216095054 1101 12-13 0.08491569405796184 1101 14-15 0.05516234911721085 1101 16-17 0.12168583327044757 1101 18-19 0.08793639830852129 1101 20-21 0.03675883678012326 1101 22-23 0.05485839002898274 1101 24-25 0.07416068245041885 1101 26-27 0.11153587954589739 1101 28-29 0.12830637611123308 1101 30-31 0.01635747015144773 1101 32-33 -0.001570455013506944 1101 34-35 0.06087798129735944 1101 36-37 0.08689132927273846 1101 38-39 0.06226232942650256 1101 40-41 0.16298708310598187 1101 42-43 0.19550047747735988 1101 44-45 0.09003250826327758 1101 46-47 0.23017578887167645 1101 48-49 0.25252400735463 1101 50-51 0.27333864708767663 1101 52-53 0.13278099895671147 1101 54-55 0.19473217301057844 1101 56-57 0.30160505356858636 1101 58-59 0.23623060696632336 1101 60-61 0.36925805461021355 1101 62-63 0.3306567416534705 1101 64-65 0.3529992670971609 1101 66-67 0.22986305005398577 1101 68-69 0.19648266732215092 1101 70-71 0.3370679145354174 1101 72-73 0.1488754125201055 1101 74-75 0.21146708853187235 1101 76-77 0.2072732152766683 1101 78-79 0.23136277563780538 1101 80-81 0.34039468777424275 1101 82-83 0.32398085599275817 1101 84-85 0.5331974237068309 1101 86-87 0.4941795535270934 1101 88-89 0.6512006203252945 1101 90-91 0.633575749805761 1101 92-93 0.4946335186657187 1101 94-95 0.4731153479592187 1101 96-97 0.6975751218213126 1101 98-99 0.927028615803767 1101 100-101 0.9962761236074016 1102 1 0.11270468594571526 1102 2 0.07468247216396406 1102 3 0.0850879954464645 1102 4 0.0882980417090451 1102 5 0.023326377153637168 1102 6 0.064064750530477 1102 7 0.0987563749052498 1102 8 0.16079724259126493 1102 9 0.08791551645402507 1102 10-11 0.1372542458926489 1102 12-13 0.10969222074992757 1102 14-15 0.2537210127387439 1102 16-17 0.2456560183366321 1102 18-19 0.22267398620686407 1102 20-21 0.1494334198842182 1102 22-23 0.2445364890668955 1102 24-25 0.2403422997337401 1102 26-27 0.12108593941616874 1102 28-29 0.20346379563309824 1102 30-31 0.17900032663385446 1102 32-33 0.11191925377193712 1102 34-35 0.20655518574857012 1102 36-37 0.3212817158174204 1102 38-39 0.37084446728069054 1102 40-41 0.5369046794185337 1102 42-43 0.4303303557488505 1102 44-45 0.3882771996886447 1102 46-47 0.4123516109899299 1102 48-49 0.412607929759055 1102 50-51 0.3815847948169946 1102 52-53 0.4249710489333651 1102 54-55 0.40984069077055096 1102 56-57 0.5371188745865183 1102 58-59 0.3683829664098752 1102 60-61 0.39545967873585397 1102 62-63 0.37449755838571264 1102 64-65 0.36344449157601133 1102 66-67 0.319819580536965 1102 68-69 0.42219446477108136 1102 70-71 0.5428667781560179 1102 72-73 0.3593587205029678 1102 74-75 0.5205641338529823 1102 76-77 0.4278376717516714 1102 78-79 0.45697604141096093 1102 80-81 0.478980212113008 1102 82-83 0.46275824259753406 1102 84-85 0.38179440199172276 1102 86-87 0.44100163248188906 1102 88-89 0.287693863537303 1102 90-91 0.33305645031108 1102 92-93 0.30170069588634973 1102 94-95 0.6104973988974933 1102 96-97 0.4521093126017526 1102 98-99 0.5091710931848219 1102 100-101 0.6074735984225903 1103 1 0.09701833366712975 1103 2 0.2044630275463959 1103 3 0.12151988149164339 1103 4 0.04320452419257492 1103 5 0.13408103319842013 1103 6 0.1129782928640779 1103 7 0.14842009713471072 1103 8 0.16672955642030018 1103 9 0.15861770523419239 1103 10-11 0.08305261521460494 1103 12-13 0.1977904807178632 1103 14-15 0.3603984070534594 1103 16-17 0.2343605415134533 1103 18-19 0.2756191788795803 1103 20-21 0.18610497820730387 1103 22-23 0.2546548520429113 1103 24-25 0.2547294356216909 1103 26-27 0.26591627070785506 1103 28-29 0.2889007300943476 1103 30-31 0.2597214398917558 1103 32-33 0.3158248353596562 1103 34-35 0.2141357422641974 1103 36-37 0.4296319999678815 1103 38-39 0.43438396787846045 1103 40-41 0.4611696561028964 1103 42-43 0.3127643042221351 1103 44-45 0.5033714537865919 1103 46-47 0.6586959005004402 1103 48-49 0.5158197082633365 1103 50-51 0.5479776987044431 1103 52-53 0.6745149350238719 1103 54-55 0.5809751962127621 1103 56-57 0.5092610801380815 1103 58-59 0.46573438942861145 1103 60-61 0.3980422466854918 1103 62-63 0.5057334287700428 1103 64-65 0.5064605660500661 1103 66-67 0.5619545902541745 1103 68-69 0.8399285135327688 1103 70-71 0.8651480842849395 1103 72-73 0.6491890801246996 1103 74-75 0.7782852074487998 1103 76-77 0.6706081246359581 1103 78-79 0.618764177916507 1103 80-81 0.7617408061376345 1103 82-83 0.9151249355167188 1103 84-85 0.973497944948587 1103 86-87 1.2444003893590079 1103 88-89 0.992449063519171 1103 90-91 1.0651051100161055 1103 92-93 1.1339578648071438 1103 94-95 0.8496421711521656 1103 96-97 1.1571350646231018 1103 98-99 1.085465351815575 1103 100-101 1.0756005850678036 1104 1 0.019934366523834512 1104 2 0.08447628322895184 1104 3 0.07109504773094244 1104 4 0.07130874428036549 1104 5 -0.09461425994773975 1104 6 -0.05945749313270454 1104 7 0.027948759831922132 1104 8 0.0039399958135817315 1104 9 0.017571810149654254 1104 10-11 -0.0362827535541399 1104 12-13 0.04231001655166722 1104 14-15 0.21559800286379271 1104 16-17 0.08999366333534198 1104 18-19 0.0950909073336561 1104 20-21 0.05202755198278197 1104 22-23 0.023937524040825053 1104 24-25 0.07348025052020546 1104 26-27 0.07004726292829133 1104 28-29 0.001506666901683218 1104 30-31 -0.016575878548856338 1104 32-33 -0.01835961420355403 1104 34-35 0.1683042198164557 1104 36-37 0.13776414391155356 1104 38-39 0.1144450309407361 1104 40-41 0.16745089387941903 1104 42-43 0.19883171262754473 1104 44-45 0.25672503282088144 1104 46-47 0.3325974172982171 1104 48-49 0.3538374404550879 1104 50-51 0.2347269055365544 1104 52-53 -0.019411897954519475 1104 54-55 0.09377779519576279 1104 56-57 0.24270668837833398 1104 58-59 0.3189396569326348 1104 60-61 0.3345937048105796 1104 62-63 0.11678834206483657 1104 64-65 0.12501689183562092 1104 66-67 0.18163438946317711 1104 68-69 0.11196282619302877 1104 70-71 -0.05030619447554585 1104 72-73 0.04614570579291666 1104 74-75 -0.02090152383026478 1104 76-77 -0.06399083664006255 1104 78-79 0.029148158098173838 1104 80-81 0.029457243184008775 1104 82-83 -0.07350885995461809 1104 84-85 0.06701732866800114 1104 86-87 -0.013198810953912954 1104 88-89 0.005659111761161739 1104 90-91 0.015765721081340445 1104 92-93 0.03445984229421484 1104 94-95 -0.0400167666547766 1104 96-97 0.27869945247133643 1104 98-99 0.08392247728821545 1104 100-101 0.08584711903277054 1105 1 0.08188393113719883 1105 2 0.10572153863097355 1105 3 0.08491226803740659 1105 4 0.02030069810374613 1105 5 0.016545778753297213 1105 6 0.0723875456096792 1105 7 0.010474374458922853 1105 8 0.015396287625996763 1105 9 -0.0023544676873825665 1105 10-11 0.09749268534701372 1105 12-13 0.1011368294222379 1105 14-15 0.10172920185161871 1105 16-17 0.09314484131875389 1105 18-19 0.15176662905007277 1105 20-21 0.13784430506522227 1105 22-23 0.16131689341243316 1105 24-25 0.028868108484104482 1105 26-27 0.18005784782661038 1105 28-29 0.05439601336690458 1105 30-31 0.13989558770764177 1105 32-33 0.1359545588099209 1105 34-35 0.21855612799246416 1105 36-37 0.14985046458436102 1105 38-39 0.03716183529377304 1105 40-41 0.1988841139212596 1105 42-43 0.16846525187023076 1105 44-45 0.08721625893250007 1105 46-47 -0.027731541495107592 1105 48-49 0.03964375335460346 1105 50-51 0.027503229269051133 1105 52-53 -0.031173642643466337 1105 54-55 0.09170319647091674 1105 56-57 0.05306748456115429 1105 58-59 0.1251533230287123 1105 60-61 0.023230754435552115 1105 62-63 -0.055496900271450045 1105 64-65 -0.03140340718774581 1105 66-67 -0.017851147265595557 1105 68-69 -0.017212730134339438 1105 70-71 0.06147043522667417 1105 72-73 -0.03008313474724389 1105 74-75 -0.16872986241470045 1105 76-77 -0.2343430946138838 1105 78-79 -0.17889574442152778 1105 80-81 -0.23928805356526794 1105 82-83 -0.03593350457492406 1105 84-85 0.08817627166666853 1105 86-87 0.09263363341334951 1105 88-89 0.031025655212996384 1105 90-91 -0.0824526255244642 1105 92-93 0.18320867423414455 1105 94-95 -0.0031313936611532256 1105 96-97 0.4298350549422487 1105 98-99 0.3851765396564204 1105 100-101 0.24605999539619816 1106 1 -0.1283998629873011 1106 2 0.01312474526563534 1106 3 0.011046552291269052 1106 4 0.005929518496515129 1106 5 0.018144256796531977 1106 6 -0.06277080503924282 1106 7 0.025430805724369066 1106 8 -0.03937237917980241 1106 9 -0.07061870503036971 1106 10-11 0.019422080103034034 1106 12-13 -0.1589939221497687 1106 14-15 -0.2863198685509829 1106 16-17 -0.1834289109761258 1106 18-19 -0.09812146236411934 1106 20-21 0.021674875466310084 1106 22-23 -0.036393063729605046 1106 24-25 -0.018429147940814516 1106 26-27 -0.13345027461952697 1106 28-29 -0.03225072147326813 1106 30-31 -0.017373782307508634 1106 32-33 -0.10938989279264177 1106 34-35 -0.15013805003896863 1106 36-37 -0.0831719443538077 1106 38-39 -0.10444486954278887 1106 40-41 -0.09251450143683115 1106 42-43 -0.26144367873737195 1106 44-45 -0.06123350469842137 1106 46-47 -0.0011585453393152534 1106 48-49 -0.10888767329839055 1106 50-51 -0.010513932063808795 1106 52-53 -0.4111365615823317 1106 54-55 -0.5119309592392938 1106 56-57 -0.4114786656322664 1106 58-59 -0.2399280224149436 1106 60-61 -0.220701229340726 1106 62-63 -0.23672604973594957 1106 64-65 -0.3169767269710704 1106 66-67 -0.17227819042903292 1106 68-69 -0.2294526560932013 1106 70-71 -0.46316079573752944 1106 72-73 -0.35883653359246637 1106 74-75 -0.4457852753120122 1106 76-77 -0.4122157426432338 1106 78-79 -0.438162229009901 1106 80-81 -0.5563530543062853 1106 82-83 -0.7071763250342364 1106 84-85 -0.49644249812527264 1106 86-87 -0.27843179681467234 1106 88-89 -0.3444775553535955 1106 90-91 -0.484426753469247 1106 92-93 -0.3789459592349864 1106 94-95 -0.4081179797536372 1106 96-97 -0.33319700233075267 1106 98-99 -0.3199655086336435 1106 100-101 -0.35552426723208086 1107 1 0.10485443303100084 1107 2 0.1274966412904064 1107 3 0.09611183064203743 1107 4 0.07815753805741821 1107 5 0.07346561890306447 1107 6 0.03131024698977569 1107 7 0.01798293061950318 1107 8 1.0773515545281498E-4 1107 9 0.026295508791022826 1107 10-11 0.005568899172295971 1107 12-13 0.03694533950561407 1107 14-15 0.06942299417961095 1107 16-17 0.0859577147300854 1107 18-19 0.03996733827197829 1107 20-21 0.01147901424270259 1107 22-23 0.07042960289709299 1107 24-25 0.0021638555675735915 1107 26-27 -0.004246391179812292 1107 28-29 -0.006373495307109067 1107 30-31 -0.013707659527966598 1107 32-33 0.06734154069491183 1107 34-35 -0.02343590461126155 1107 36-37 0.03573258228119869 1107 38-39 0.1564984201130244 1107 40-41 0.08449696492187542 1107 42-43 0.18176437022103897 1107 44-45 0.16108951666831217 1107 46-47 0.11868457915183228 1107 48-49 0.07923779094472394 1107 50-51 0.10048971811826846 1107 52-53 0.007815691980411543 1107 54-55 0.06610209177712534 1107 56-57 -0.09766245760567216 1107 58-59 0.029314423240357712 1107 60-61 0.05986696377869549 1107 62-63 0.0874687427470775 1107 64-65 0.18002166325627655 1107 66-67 -0.030260840287063218 1107 68-69 0.13405308898219914 1107 70-71 0.04729106834874841 1107 72-73 0.017372282898691083 1107 74-75 -0.21082675205833823 1107 76-77 -0.21829238457089062 1107 78-79 -0.024917697264356775 1107 80-81 -0.014599606000068377 1107 82-83 -0.04990301247615392 1107 84-85 0.020488903100211786 1107 86-87 0.04872915523047183 1107 88-89 0.11561645194296588 1107 90-91 0.19108176671524646 1107 92-93 0.32621416389381963 1107 94-95 0.26920373542747456 1107 96-97 0.3619392763678597 1107 98-99 0.6011337429148611 1107 100-101 0.907556741495334 1108 1 0.03682855103444638 1108 2 0.01324441497723683 1108 3 0.017829313352223153 1108 4 -0.010079629588105377 1108 5 -0.015205180238737626 1108 6 0.035581446450350995 1108 7 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-0.6635352736179314 2316 54-55 -0.7528175657432854 2316 56-57 -0.7026627511793464 2316 58-59 -0.7852525728620137 2316 60-61 -0.6586640971792832 2316 62-63 -0.5980389497465808 2316 64-65 -0.6581674361805767 2316 66-67 -0.7533099137167554 2316 68-69 -0.6984471571016115 2316 70-71 -0.6297055780037724 2316 72-73 -0.7439175675687011 2316 74-75 -0.6963455830825147 2316 76-77 -0.6118736552613555 2316 78-79 -0.5321035604832005 2316 80-81 -0.46401491949357876 2316 82-83 -0.39934634498632704 2316 84-85 -0.36546182660305604 2316 86-87 -0.46525963025524675 2316 88-89 -0.5693209305196341 2316 90-91 -0.5931886422674175 2316 92-93 -0.9500507500804574 2316 94-95 -0.7149877540444542 2316 96-97 -0.7684432673962043 2316 98-99 -0.6048073247607775 2316 100-101 -0.44393146116030024 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 16.0 5 32.0 6 58.0 7 153.0 8 362.0 9 571.0 10 1044.0 11 1640.0 12 2539.0 13 3853.0 14 5199.0 15 7020.0 16 9437.0 17 14307.0 18 14310.0 19 8976.0 20 6934.0 21 6922.0 22 7316.0 23 7761.0 24 8722.0 25 10390.0 26 12255.0 27 14447.0 28 17975.0 29 22102.0 30 27086.0 31 33464.0 32 42631.0 33 55512.0 34 79302.0 35 129376.0 36 197512.0 37 319598.0 38 282539.0 39 30007.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.35933576135593 18.821373838020346 14.78900309909503 22.0302873015287 2 16.036761122933125 20.448863360733544 39.65215515087975 23.86222036545358 3 19.20948416289593 25.29419004524887 29.722425339366517 25.77390045248869 4 12.970375083884052 17.195235747673866 35.89911761576 33.93527155268208 5 13.635004643937073 37.7806541472204 33.109497738116694 15.474843470725821 6 31.747227935145588 35.87457677915636 16.960613767047374 15.417581518650675 7 28.72472348123712 33.77115923238579 19.638026279688802 17.866091006688283 8 27.349397154264075 34.30556008006573 18.93373583375864 19.41130693191155 9 27.484665507907767 16.59751998569529 19.646103160429227 36.27171134596772 10-11 25.643726413830898 26.874170660886904 25.733673285453364 21.748429639828835 12-13 26.618193084985858 24.49747388645049 26.885101829993534 21.999231198570115 14-15 23.3375031038005 25.42937415075313 26.040506134847192 25.192616610599178 16-17 21.88193153453678 28.9632089349109 26.517734593533365 22.637124937018953 18-19 21.732720608728577 26.604293850095306 30.108421271331164 21.55456426984496 20-21 22.745880722847083 25.603042195036668 30.359005654527277 21.29207142758897 22-23 22.48570441973952 25.615927944049787 30.472914227966275 21.42545340824442 24-25 22.02742994735758 25.943630658209422 30.51421468391563 21.51472471051737 26-27 21.60788556003522 26.301286841373557 30.425630821632144 21.665196776959082 28-29 21.564453562603227 26.345792095603603 30.618577709600718 21.47117663219245 30-31 21.73301017612213 26.123756760493745 30.456408886533744 21.68682417685039 32-33 21.459881016327316 26.3312909105914 30.292152840014886 21.9166752330664 34-35 21.937243342518066 26.385194662357048 30.183444820836197 21.49411717428869 36-37 21.974953549646767 26.054005842989298 30.09476125441473 21.876279352949204 38-39 21.611821336098284 26.22366584220069 30.30955125494535 21.85496156675568 40-41 21.800433839479393 26.174812982747785 30.290712996938794 21.73404018083403 42-43 21.853523841172034 26.162465959411563 29.904713501593292 22.079296697823104 44-45 21.678270800115108 26.41597877411847 29.901634834480472 22.00411559128595 46-47 21.913687829360644 26.653875013166285 29.377440286614114 22.054996870858957 48-49 21.93440429833839 26.8184651335521 29.109051804330626 22.138078763778886 50-51 21.939688903270735 27.024456138141023 28.886953613475306 22.148901345112932 52-53 22.223283969331916 26.997960721630903 28.70050840795123 22.078246901085947 54-55 21.910732163915412 26.98695716067588 28.69591152557857 22.406399149830133 56-57 21.991268095247403 27.222882194573362 28.607774450165813 22.178075260013422 58-59 22.00947464511706 26.896612133887277 28.637853263171152 22.456059957824507 60-61 21.981603783487564 27.046390868202675 28.840984789748735 22.131020558561023 62-63 21.866935095640493 27.087979985101757 28.880184975651 22.16489994360675 64-65 21.790308324121472 26.95673356397376 28.780465204496114 22.472492907408654 66-67 21.824875431726888 27.221036202439457 28.594309566365588 22.359778799468064 68-69 21.786097058028282 27.108568706634973 28.65362960453376 22.451704630802983 70-71 21.815717862905764 27.14249104693815 28.47681759643137 22.564973493724715 72-73 21.826612836088206 27.2529972035029 28.28512398189914 22.635265978509757 74-75 21.71690298985573 27.51201523703625 28.156845626555064 22.614236146552955 76-77 21.750709983053067 27.231677804152543 28.37228376735866 22.64532844543573 78-79 21.746438863998158 27.27514910910972 28.303837274707515 22.674574752184608 80-81 21.65667297199666 27.651043781865987 28.209727871802727 22.482555374334627 82-83 21.90932845315576 27.51419695150577 28.047001148497092 22.52947344684138 84-85 21.725503170105593 27.434118415280214 27.950645097303926 22.889733317310267 86-87 21.831570405102486 27.31136161185319 28.108543243230528 22.7485247398138 88-89 21.758898137755224 27.369666573884416 27.917636868602596 22.95379841975777 90-91 21.70963856119441 27.380574908351722 28.100332424089743 22.80945410636413 92-93 21.774188586037262 27.15417562064314 28.170847843390202 22.9007879499294 94-95 21.521517391055696 27.12620288105078 28.205999691610728 23.146280036282793 96-97 21.79830762336838 26.96672363905135 28.130458797817532 23.104509939762742 98-99 21.57219168492751 27.08189906983714 27.969169759608388 23.37673948562696 100-101 21.61099371566364 26.897444640143743 28.23390674916442 23.257654895028203 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3632.0 1 2341.0 2 1668.5 3 2156.5 4 1595.5 5 1356.5 6 2508.0 7 5330.5 8 8050.5 9 8447.0 10 6085.0 11 3642.5 12 3089.5 13 3091.5 14 3119.5 15 3025.5 16 2889.5 17 2925.5 18 3010.5 19 3046.0 20 3166.5 21 3451.5 22 3839.0 23 4363.5 24 4995.5 25 5857.0 26 6817.5 27 7844.0 28 8962.5 29 10507.0 30 12761.5 31 15014.0 32 17711.5 33 20152.0 34 21876.0 35 23907.5 36 27040.0 37 30137.0 38 32071.5 39 33494.0 40 35120.5 41 37945.5 42 40106.0 43 42604.5 44 45985.5 45 49180.5 46 50737.0 47 51246.0 48 53404.0 49 53303.0 50 51490.0 51 49722.5 52 47892.5 53 47781.0 54 47830.5 55 45782.5 56 42011.5 57 38531.0 58 35347.0 59 31150.0 60 26199.5 61 21621.0 62 17553.5 63 13406.5 64 10183.5 65 8017.5 66 6166.5 67 5063.0 68 3818.5 69 2441.0 70 1706.5 71 1263.0 72 973.0 73 815.5 74 619.0 75 446.5 76 299.5 77 181.0 78 115.5 79 64.5 80 36.0 81 18.0 82 10.0 83 7.0 84 3.0 85 2.0 86 2.0 87 2.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.017229259916416374 3 0.008759413654984794 4 0.0 5 0.0 6 0.0 7 0.0 8 4.343510903298245E-4 9 5.791347871064327E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.1717554516491224E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005103625311375438 26-27 0.022803432242315787 28-29 3.619592419415204E-5 30-31 0.0 32-33 3.6195924194152044E-4 34-35 0.019871562382589473 36-37 0.004162531282327485 38-39 0.019907758306783624 40-41 0.015564247403485377 42-43 0.008361258488849123 44-45 0.0027870861629497074 46-47 1.0858777258245612E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.0858777258245612E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.619592419415204E-5 84-85 0.0020993636032608184 86-87 3.2576331774736837E-4 88-89 3.619592419415204E-5 90-91 2.1717554516491224E-4 92-93 1.0858777258245612E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1381371.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.989374908905575 #Duplication Level Percentage of deduplicated Percentage of total 1 75.81869063358589 40.1758502308541 2 13.349751223763043 14.147899450732016 3 4.4858802610520785 7.131119728480434 4 2.041546391800957 4.327210685962574 5 1.1268212631116123 2.9854777183173877 6 0.6840426645056631 2.1748195921906377 7 0.4460664667376507 1.6545748268176559 8 0.30692464186360213 1.301099593319359 9 0.24405689041659073 1.1639179858847768 >10 1.3468210153741365 13.161284851396305 >50 0.10090939310691989 3.63891963485097 >100 0.0457573911735096 4.316453221012552 >500 0.0013658817541650585 0.4388954642186872 >1k 9.56117227915541E-4 1.0328953116531803 >5k 1.3658817541650585E-4 0.5501392519007654 >10k+ 2.731763508330117E-4 1.7994424524084764 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13671 0.9896689593165052 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11191 0.8101371753135109 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7601 0.5502504395994993 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3587 0.25966956016884674 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2602 0.18836358950636722 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2395 0.17337847688998828 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1954 0.14145367175074616 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.239184838830409E-5 0.0 14-15 0.0 0.0 0.0 7.239184838830409E-5 0.0 16-17 0.0 0.0 0.0 1.4478369677660817E-4 0.0 18-19 0.0 0.0 0.0 1.4478369677660817E-4 0.0 20-21 0.0 0.0 0.0 2.5337146935906427E-4 0.0 22-23 0.0 0.0 0.0 7.963103322713449E-4 0.0 24-25 0.0 0.0 0.0 0.001665012512930994 0.0 26-27 0.0 0.0 0.0 0.0035472005710269 0.0 28-29 0.0 0.0 0.0 0.009266156593702923 0.0 30-31 0.0 0.0 0.0 0.02265864854553918 0.0 32-33 0.0 0.0 0.0 0.04571545225721403 0.0 34-35 0.0 0.0 0.0 0.08024636393843508 0.0 36-37 0.0 0.0 0.0 0.13580710757645847 0.0 38-39 0.0 0.0 0.0 0.21706695739232978 0.0 40-41 0.0 0.0 0.0 0.304480114321207 0.0 42-43 0.0 0.0 0.0 0.3896853198742409 0.0 44-45 0.0 0.0 0.0 0.4873781192742572 0.0 46-47 0.0 0.0 0.0 0.6066436894939882 0.0 48-49 0.0 0.0 0.0 0.730868101328318 0.0 50-51 0.0 0.0 0.0 0.8635985553482736 0.0 52-53 0.0 0.0 0.0 0.9931075721149496 0.0 54-55 0.0 0.0 0.0 1.1281183693591368 0.0 56-57 0.0 0.0 0.0 1.2852448762859507 0.0 58-59 0.0 0.0 0.0 1.457465083601726 0.0 60-61 0.0 0.0 0.0 1.6397115619192817 0.0 62-63 0.0 0.0 0.0 1.8161304964415788 0.0 64-65 0.0 0.0 0.0 1.9916807287832161 0.0 66-67 0.0 0.0 0.0 2.1846050047380463 0.0 68-69 0.0 0.0 0.0 2.397364647151272 0.0 70-71 0.0 0.0 0.0 2.6290909538422333 0.0 72-73 0.0 0.0 0.0 2.8560032026153728 0.0 74-75 0.0 0.0 0.0 3.0771241035174475 0.0 76-77 0.0 0.0 0.0 3.3153656765633563 0.0 78-79 0.0 0.0 0.0 3.5779309106677353 0.0 80-81 0.0 0.0 0.0 3.8572910535982006 0.0 82-83 0.0 0.0 0.0 4.14182721368843 0.0 84-85 0.0 0.0 0.0 4.436389644780439 0.0 86-87 0.0 0.0 0.0 4.7354403704725225 0.0 88-89 0.0 0.0 0.0 5.051430788687471 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4650 0.0 54.145046 1 GTATCAA 8455 0.0 42.026558 1 TATCAAC 10815 0.0 32.63609 2 ATCAACG 10970 0.0 32.040405 3 TCAACGC 11075 0.0 31.778376 4 CAACGCA 11250 0.0 31.241827 5 AACGCAG 11640 0.0 30.15426 6 GTGGTAT 2135 0.0 28.035513 1 TGGTATC 2150 0.0 26.514204 2 ACGCAGA 13215 0.0 26.48851 7 CGCAGAG 13305 0.0 26.237936 8 GTGTAAG 470 0.0 25.268412 1 GCAGAGT 13995 0.0 24.571001 9 GTACATG 8275 0.0 24.340734 1 TACATGG 8410 0.0 23.441635 2 GAGTACT 8275 0.0 23.417974 12-13 GTACTTT 8620 0.0 22.370514 14-15 ACATGGG 8660 0.0 22.158298 3 TAAGGTA 345 0.0 22.02714 4 AGTACTT 8465 0.0 22.022667 12-13 >>END_MODULE