FastQCFastQC Report
Thu 26 May 2016
SRR938216_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938216_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1404789
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138200.9837776349330754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107560.7656665876512415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71320.5076919024849995No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38140.2714998480198806No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24640.17540000669139635No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24000.1708441623617497No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20600.14664123936050183No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14420.10264886755235128No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45950.052.9801371
GTATCAA81550.042.7042471
TATCAAC107150.032.4048652
ATCAACG107850.032.1945423
TCAACGC109150.031.8546734
CAACGCA111050.031.309665
AACGCAG113500.030.6713396
GTGGTAT21050.028.290171
ACGCAGA129900.026.7153437
CGCAGAG131350.026.4928138
TGGTATC21850.025.904492
GTAAGGT5250.025.3675354
GCAGAGT138100.024.9913659
GTACATG90900.024.894731
TAAGGTG6200.024.5492275
TACATGG91800.024.3520052
GAGTACT80300.024.036612-13
ACATGGG92100.023.4463773
CATGGGG38750.022.7080354
GTACTTT85900.022.19050814-15
Produced by FastQC (version 0.11.5)