##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938216_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1404789 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06546250006229 33.0 31.0 34.0 30.0 34.0 2 32.157978173234554 34.0 31.0 34.0 30.0 34.0 3 32.17049962663432 34.0 31.0 34.0 30.0 34.0 4 35.70202856087284 37.0 35.0 37.0 35.0 37.0 5 35.709259540044805 37.0 35.0 37.0 35.0 37.0 6 35.75388047600031 37.0 36.0 37.0 35.0 37.0 7 35.69600060934418 37.0 35.0 37.0 35.0 37.0 8 35.694150509435936 37.0 35.0 37.0 35.0 37.0 9 37.336725301806894 39.0 37.0 39.0 35.0 39.0 10-11 37.25163601081728 39.0 37.0 39.0 34.0 39.0 12-13 37.09678962463401 39.0 37.0 39.0 33.5 39.0 14-15 38.26737467334952 40.0 38.0 41.0 33.5 41.0 16-17 38.11414845930599 40.0 38.0 41.0 33.0 41.0 18-19 38.30995046231142 40.0 38.0 41.0 34.0 41.0 20-21 38.32847851171955 40.0 38.0 41.0 34.0 41.0 22-23 38.206081838624876 40.0 38.0 41.0 34.0 41.0 24-25 38.083012466640895 40.0 38.0 41.0 33.5 41.0 26-27 37.90289253403892 40.0 38.0 41.0 33.0 41.0 28-29 37.718593682040506 40.0 37.5 41.0 33.0 41.0 30-31 37.46450926082137 40.0 37.0 41.0 32.5 41.0 32-33 37.14748371463615 40.0 36.0 41.0 31.5 41.0 34-35 36.89772200665011 39.0 35.5 41.0 31.0 41.0 36-37 36.64212703829543 39.0 35.0 41.0 30.5 41.0 38-39 36.34897198084552 39.0 35.0 40.5 30.0 41.0 40-41 36.203971557294366 39.0 35.0 41.0 30.0 41.0 42-43 35.959572932305136 39.0 35.0 41.0 29.5 41.0 44-45 35.58134531235652 39.0 35.0 40.0 27.5 41.0 46-47 35.13064346318202 38.0 35.0 40.0 25.5 41.0 48-49 34.67879482256766 38.0 34.0 40.0 24.0 41.0 50-51 33.94060282362689 37.5 33.5 39.5 23.0 40.5 52-53 34.00906043541059 38.0 33.5 40.0 23.0 40.5 54-55 34.09106492149355 38.0 34.0 40.0 22.5 41.0 56-57 33.7864010182312 38.0 33.5 40.0 21.0 41.0 58-59 33.38622277082181 37.0 33.0 40.0 19.0 41.0 60-61 32.906950438820346 36.5 32.5 40.0 16.0 41.0 62-63 32.37182452311343 36.0 32.0 39.0 11.0 41.0 64-65 31.8586371334058 35.5 31.0 39.0 7.5 40.0 66-67 31.317138374517455 35.0 31.0 38.5 4.0 40.0 68-69 30.665221965718693 35.0 30.0 38.0 2.0 40.0 70-71 30.099714974989126 35.0 29.0 37.0 2.0 39.5 72-73 29.423289191472882 34.0 28.5 36.5 2.0 39.0 74-75 28.769709187643127 34.0 27.5 36.0 2.0 39.0 76-77 28.055288730193645 34.0 26.0 35.5 2.0 38.0 78-79 27.325457417448455 33.0 25.5 35.0 2.0 37.0 80-81 26.609086133220007 33.0 24.5 35.0 2.0 36.5 82-83 25.892584936243097 32.0 21.0 35.0 2.0 36.0 84-85 25.17616346654195 31.5 19.0 35.0 2.0 35.5 86-87 24.449564311793445 31.0 14.0 34.0 2.0 35.0 88-89 23.594010559592938 31.0 4.5 34.0 2.0 35.0 90-91 22.704051640495475 30.0 2.0 34.0 2.0 35.0 92-93 21.771525830569573 29.0 2.0 34.0 2.0 35.0 94-95 20.701141594929915 29.0 2.0 34.0 2.0 35.0 96-97 19.44328187364793 26.5 2.0 33.5 2.0 35.0 98-99 17.901050620413457 24.0 2.0 33.0 2.0 35.0 100-101 13.934896628604012 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14193753491348104 1101 2 0.15430260919294625 1101 3 0.15971246986364207 1101 4 -0.01885948606226151 1101 5 0.13939862220306054 1101 6 0.07654526241557136 1101 7 0.10209452628899385 1101 8 0.11285608055226248 1101 9 0.20985422789241426 1101 10-11 0.21072910648424426 1101 12-13 0.3272867646055886 1101 14-15 0.32221407737966956 1101 16-17 0.3890283103652692 1101 18-19 0.43332230626456436 1101 20-21 0.4720541423250211 1101 22-23 0.5090481674714198 1101 24-25 0.4669322306981911 1101 26-27 0.36821986153497477 1101 28-29 0.33859937207148505 1101 30-31 0.3010601013794485 1101 32-33 0.29013642326249567 1101 34-35 0.21019979385949483 1101 36-37 0.3207796676729302 1101 38-39 0.41884821061659494 1101 40-41 0.46296114176165304 1101 42-43 0.3130400128510118 1101 44-45 0.33163781047412044 1101 46-47 0.2961820409136209 1101 48-49 0.3566768385974939 1101 50-51 0.37627740034862 1101 52-53 0.5921628708037403 1101 54-55 0.40773881453037575 1101 56-57 0.23838460865275124 1101 58-59 0.25566909442519403 1101 60-61 0.3117468494502944 1101 62-63 0.17743138351215748 1101 64-65 0.1605844328000856 1101 66-67 0.14388731468811145 1101 68-69 0.09598076867066396 1101 70-71 0.14454428237264594 1101 72-73 0.06312498397409172 1101 74-75 0.03629484602485533 1101 76-77 -0.3345168949129409 1101 78-79 -0.4527737623257373 1101 80-81 -0.20081122588985423 1101 82-83 -0.0010050770889868943 1101 84-85 -0.31223270723041807 1101 86-87 -0.28843183860477595 1101 88-89 -0.35348325410566517 1101 90-91 -0.4637453704100736 1101 92-93 -0.8042609002907142 1101 94-95 -1.0948215040968918 1101 96-97 -1.2283559888920301 1101 98-99 -1.2362344058444563 1101 100-101 -1.331937215986155 1102 1 0.17165562009915902 1102 2 0.2809033814397708 1102 3 0.23952224072399986 1102 4 0.11478990195449512 1102 5 0.2067510678997948 1102 6 0.21015588900888815 1102 7 0.1624997322463244 1102 8 0.25670270458136457 1102 9 0.21292830693590048 1102 10-11 0.20418438921900872 1102 12-13 0.15639659360807912 1102 14-15 0.20345354843532704 1102 16-17 0.1736087179565473 1102 18-19 0.12945809051252155 1102 20-21 0.24288318953941967 1102 22-23 0.21623393904737043 1102 24-25 0.2983230203876559 1102 26-27 0.2951888673044607 1102 28-29 0.22900144729845806 1102 30-31 0.31121261267244904 1102 32-33 0.3419425565123362 1102 34-35 0.32991594097400423 1102 36-37 0.39436077595147623 1102 38-39 0.17124918263382227 1102 40-41 0.15115530667660693 1102 42-43 0.3602141559999339 1102 44-45 0.25403594992579315 1102 46-47 0.44201295165798626 1102 48-49 0.4650865766597221 1102 50-51 0.4409836334836754 1102 52-53 0.33605272872821956 1102 54-55 0.17163553024514044 1102 56-57 0.15597185912608325 1102 58-59 0.31560021835738183 1102 60-61 0.3861762365725525 1102 62-63 0.06053140289287029 1102 64-65 0.06640759125976814 1102 66-67 0.08327935666971698 1102 68-69 0.008414240650623128 1102 70-71 -0.4567736060242673 1102 72-73 -0.5357404989783348 1102 74-75 -0.5529607855627212 1102 76-77 -0.5275726368214926 1102 78-79 -0.7255915687273351 1102 80-81 -0.8041745247851537 1102 82-83 -0.9489827743622712 1102 84-85 -0.8449503302606693 1102 86-87 -0.7629655834055278 1102 88-89 -0.7766521495541845 1102 90-91 -1.1077708933165873 1102 92-93 -1.2154718966083813 1102 94-95 -1.5599690211295574 1102 96-97 -1.7871391783608495 1102 98-99 -1.667908452089815 1102 100-101 -1.716945490059235 1103 1 0.11710634420585109 1103 2 0.09076282038844141 1103 3 0.17089527424677442 1103 4 0.03934647713860784 1103 5 0.07213680843698711 1103 6 0.06969424436790206 1103 7 0.09635111641339478 1103 8 0.013451708384380368 1103 9 -0.047493915854353475 1103 10-11 0.08616962590632227 1103 12-13 -0.03656598119544441 1103 14-15 0.03315257339776423 1103 16-17 0.03729204802365871 1103 18-19 0.04070780294318155 1103 20-21 0.09138853903912292 1103 22-23 -0.047506478858331036 1103 24-25 0.03452892287253917 1103 26-27 0.010490112160162823 1103 28-29 0.07590038478301153 1103 30-31 0.14546105553773714 1103 32-33 0.13812931611005297 1103 34-35 0.1054070809982619 1103 36-37 0.15589805771829646 1103 38-39 0.17972772073073173 1103 40-41 0.3525080896377375 1103 42-43 0.3545516672541922 1103 44-45 0.16887623178634215 1103 46-47 0.36222346661301685 1103 48-49 0.4909385629472851 1103 50-51 0.5122397647459422 1103 52-53 0.36034971045727104 1103 54-55 0.267659262880926 1103 56-57 0.3885286842470066 1103 58-59 0.3589844195110743 1103 60-61 0.36387221685026816 1103 62-63 0.2518759208982573 1103 64-65 0.1654157965714944 1103 66-67 0.19407350246807553 1103 68-69 0.27205354775763624 1103 70-71 0.09279965528182288 1103 72-73 0.17968372614656403 1103 74-75 -0.04816143085411184 1103 76-77 -0.2003440099686209 1103 78-79 -0.21918381386989694 1103 80-81 0.03881432178489064 1103 82-83 -0.05901461290309129 1103 84-85 -0.3167017908288763 1103 86-87 -0.5263509774394244 1103 88-89 -0.4000016435741607 1103 90-91 -0.569342541394203 1103 92-93 -0.7471201446365789 1103 94-95 -0.5182136694997155 1103 96-97 -0.7055083975071454 1103 98-99 -0.9899562865862066 1103 100-101 -0.9610658687134954 1104 1 0.2729412371050728 1104 2 0.2596853680339066 1104 3 0.22486604277015232 1104 4 0.19541047674918133 1104 5 0.23061251333696475 1104 6 0.2617692686145858 1104 7 0.19360559259238386 1104 8 0.2510253148679453 1104 9 0.3321009832672601 1104 10-11 0.40285118715480195 1104 12-13 0.35743115840990924 1104 14-15 0.304442169042936 1104 16-17 0.2668934817131401 1104 18-19 0.3978132063165063 1104 20-21 0.2885459755625348 1104 22-23 0.5152502410319926 1104 24-25 0.5090427854159145 1104 26-27 0.45303670997841294 1104 28-29 0.5046616899822567 1104 30-31 0.2897427266304504 1104 32-33 0.3319070318050308 1104 34-35 0.43534720107557234 1104 36-37 0.432939131313411 1104 38-39 0.5319980729994924 1104 40-41 0.592041537365688 1104 42-43 0.5275732673079503 1104 44-45 0.4826952819300274 1104 46-47 0.19389379210856816 1104 48-49 0.28398112652968877 1104 50-51 0.09545047572280652 1104 52-53 0.2486242088696642 1104 54-55 0.04188174190020533 1104 56-57 -0.04318719803342219 1104 58-59 -0.03446746728587158 1104 60-61 -0.07415177215684565 1104 62-63 0.1617883047774029 1104 64-65 0.004892417369198654 1104 66-67 -0.036013923171449136 1104 68-69 0.046022845341855856 1104 70-71 -0.055794830601129775 1104 72-73 -0.1263202983006586 1104 74-75 -0.12564368037513063 1104 76-77 -0.5129172592996873 1104 78-79 -0.6757273502298737 1104 80-81 -0.5118439234905097 1104 82-83 -0.8587803737781243 1104 84-85 -0.8348877312960532 1104 86-87 -0.9982433406725058 1104 88-89 -0.7274057163824743 1104 90-91 -0.6033807614731579 1104 92-93 -0.78231659702762 1104 94-95 -1.1083843566452387 1104 96-97 -1.0757720924534127 1104 98-99 -1.1882264515483278 1104 100-101 -1.405618605321731 1105 1 0.12299742743590514 1105 2 0.1078752636473368 1105 3 0.25484412577065285 1105 4 0.13309688584199364 1105 5 0.1804054075142787 1105 6 0.12890117409394009 1105 7 0.06135355163161904 1105 8 0.07584194700437763 1105 9 0.10715630467544912 1105 10-11 0.08301587444088199 1105 12-13 0.1960397336941213 1105 14-15 0.17938808175676257 1105 16-17 0.274272523903484 1105 18-19 0.2245837810194189 1105 20-21 0.08683931074957485 1105 22-23 0.001984688701938353 1105 24-25 -0.03894718243142847 1105 26-27 -0.024734233953658702 1105 28-29 -0.08875907963638241 1105 30-31 -0.028831514875641062 1105 32-33 -0.04401585219203952 1105 34-35 -0.08569634227509937 1105 36-37 -0.18193445681323084 1105 38-39 -0.06588744782769851 1105 40-41 0.15436293598357054 1105 42-43 0.01050712290156497 1105 44-45 0.1686986256450922 1105 46-47 0.1471641398841541 1105 48-49 -0.08303164839617949 1105 50-51 0.003951914104597165 1105 52-53 -0.03767245290585208 1105 54-55 0.017437059026747193 1105 56-57 0.08438248326590525 1105 58-59 0.12086794440967452 1105 60-61 0.0030385299612660788 1105 62-63 0.09376198825886206 1105 64-65 0.10007269237188154 1105 66-67 0.049592083627807426 1105 68-69 -0.13698359677505323 1105 70-71 -0.21025527497572583 1105 72-73 -0.3265916036541796 1105 74-75 -0.5098298927640066 1105 76-77 -0.6335212033521493 1105 78-79 -0.7428794550123747 1105 80-81 -0.7121507413346038 1105 82-83 -0.5711757945306175 1105 84-85 -0.5578197835603653 1105 86-87 -0.866808086994947 1105 88-89 -0.8083553052687265 1105 90-91 -1.0371340992694797 1105 92-93 -1.1333789745913805 1105 94-95 -1.2203794181422936 1105 96-97 -1.5091602308785816 1105 98-99 -1.4950971865863636 1105 100-101 -1.391765207934947 1106 1 0.14083851164658512 1106 2 0.1415336105429006 1106 3 0.18791226339973122 1106 4 0.2511508371331601 1106 5 0.22373754064981455 1106 6 0.14994477007855522 1106 7 0.11557503133147407 1106 8 0.13558299232865068 1106 9 0.3739072732350692 1106 10-11 0.2810332437033338 1106 12-13 0.26853984171192025 1106 14-15 0.377681400495689 1106 16-17 0.0023897016143763494 1106 18-19 0.3419985549741611 1106 20-21 0.4587515138806495 1106 22-23 0.5191442525980747 1106 24-25 0.5041608497722763 1106 26-27 0.3457025900349322 1106 28-29 0.06600825001955002 1106 30-31 0.027797177532868034 1106 32-33 0.14211927087624332 1106 34-35 0.08433145430577582 1106 36-37 0.11337555427659396 1106 38-39 0.12239938984314591 1106 40-41 0.13905897389061295 1106 42-43 0.04962224107304536 1106 44-45 0.18740212398162015 1106 46-47 0.14277141596325293 1106 48-49 0.4289836757292349 1106 50-51 0.46907339812516824 1106 52-53 0.4878128350696116 1106 54-55 0.44761175981743406 1106 56-57 0.22870780407878044 1106 58-59 0.29964431875616526 1106 60-61 0.6653051755044501 1106 62-63 0.5453198930015617 1106 64-65 0.21421899168200298 1106 66-67 0.14044254378363874 1106 68-69 0.14685665209796284 1106 70-71 -0.04430507838332787 1106 72-73 -0.2617253296922293 1106 74-75 -0.2253902171054527 1106 76-77 -0.6701795646500948 1106 78-79 -0.5645437932953214 1106 80-81 -0.432919540032632 1106 82-83 -0.3786926813135558 1106 84-85 -0.4901188344439511 1106 86-87 -0.8016566460374754 1106 88-89 -0.85413123314164 1106 90-91 -0.6663086992435545 1106 92-93 -0.7447580018550752 1106 94-95 -0.8486561741516851 1106 96-97 -0.8568162445896377 1106 98-99 -0.8807210253950011 1106 100-101 -0.8210740380301473 1107 1 0.03044617180806597 1107 2 0.12381678226089576 1107 3 0.04394017754749768 1107 4 0.10825744916166968 1107 5 0.053529401728241055 1107 6 0.1041482566421692 1107 7 0.16033341977632176 1107 8 -0.007977413941212319 1107 9 0.05738950907174711 1107 10-11 0.19491890930157751 1107 12-13 0.15867792477815357 1107 14-15 0.0383811475913447 1107 16-17 0.21807220523212578 1107 18-19 0.25142742393725825 1107 20-21 0.15624059301131155 1107 22-23 0.2593644281434422 1107 24-25 0.05434626787589991 1107 26-27 0.09815954896163959 1107 28-29 0.049942467841859184 1107 30-31 0.05625500201455225 1107 32-33 0.11637793656916529 1107 34-35 0.228404701895073 1107 36-37 0.02174817569096632 1107 38-39 0.07424423911486855 1107 40-41 0.0575575065044589 1107 42-43 0.148690958023046 1107 44-45 0.05116809955674029 1107 46-47 -0.22763201379831344 1107 48-49 -0.21064143077460074 1107 50-51 -0.08333406187672665 1107 52-53 -0.04899407855071303 1107 54-55 -0.17369631013022513 1107 56-57 -0.19855690697463046 1107 58-59 -0.18465343215334684 1107 60-61 -0.28178534261135013 1107 62-63 -0.370951728967313 1107 64-65 -0.5151536162080674 1107 66-67 -0.550311435740575 1107 68-69 -0.6615887300310348 1107 70-71 -0.7497978361226068 1107 72-73 -0.7509359165114518 1107 74-75 -0.7418763344152026 1107 76-77 -0.7049627025573457 1107 78-79 -0.9886286717795194 1107 80-81 -0.9999218797359077 1107 82-83 -1.1876465769871345 1107 84-85 -1.1234326927904696 1107 86-87 -1.212941589438298 1107 88-89 -1.219378930630178 1107 90-91 -1.3120402960572335 1107 92-93 -1.1471766870229807 1107 94-95 -1.150021046144218 1107 96-97 -1.7070671202878316 1107 98-99 -1.680865194394606 1107 100-101 -1.4264905187377384 1108 1 -0.22310502265806065 1108 2 -0.11184220091818986 1108 3 -0.06251709449626475 1108 4 -0.03527153586567522 1108 5 -0.05550158543824324 1108 6 0.07089599576804773 1108 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2316 100-101 -0.48152262641118604 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4268.0 3 1255.0 4 1107.0 5 1182.0 6 1482.0 7 1825.0 8 2248.0 9 2824.0 10 3748.0 11 4718.0 12 6311.0 13 7939.0 14 9315.0 15 11692.0 16 14659.0 17 19128.0 18 18683.0 19 14305.0 20 14459.0 21 15188.0 22 16888.0 23 18698.0 24 21151.0 25 23674.0 26 26839.0 27 31064.0 28 35776.0 29 41495.0 30 48715.0 31 58464.0 32 71155.0 33 89625.0 34 117664.0 35 160643.0 36 187048.0 37 195910.0 38 98286.0 39 5358.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.09876049466151 18.42298306810186 14.393075822481336 22.085180614755295 2 15.834095999829067 20.13980834220638 40.06751824167685 23.958577416287707 3 19.17161762996858 25.329373038003293 29.417170675087284 26.081838656940842 4 12.743115571442317 16.84465621514129 36.119416814862596 34.292811398553795 5 13.661071228606989 37.6511285381606 33.07741960792705 15.610380625305359 6 31.718818319092644 35.61469618800416 17.212648781820423 15.453836711082769 7 28.70021698346633 33.73804113059581 19.740432726212216 17.821309159725644 8 27.37962687912146 33.9618501746355 19.075981224991647 19.582541721251392 9 27.628469203027667 16.527589696805553 19.73964050004989 36.104300600116886 10-11 25.605009169153874 26.817895651572865 25.832002288814632 21.74509289045863 12-13 26.69838765505065 24.47865517077895 26.915798100234095 21.907159073936306 14-15 23.465663824877648 25.375112229815294 26.001424993415018 25.157798951892037 16-17 21.998386874303435 28.84886052949443 26.55156424363972 22.601188352562414 18-19 21.86102606193465 26.544134077814086 30.10164678410759 21.493193076143672 20-21 22.90226077336882 25.571287743410743 30.3217487438762 21.204702739344242 22-23 22.594862654517456 25.535272399704294 30.37580599778969 21.49405894798856 24-25 22.073827449640323 25.843138695686758 30.44398699760262 21.639046857070294 26-27 21.677983422844367 26.167210909426625 30.41020137041527 21.744604297313742 28-29 21.663283502942924 26.33817905013877 30.499220969533 21.499316477385307 30-31 21.71724764968014 26.083598280208577 30.397398936602286 21.801755133508998 32-33 21.4720577020391 26.335892008239075 30.299272310005914 21.89277797971591 34-35 22.0093829009473 26.365851912044835 30.02880990202781 21.595955284980057 36-37 22.067192740972523 26.00437872790331 29.982851288540076 21.94557724258409 38-39 21.71269156068228 26.25744359745566 30.128790884143758 21.9010739577183 40-41 21.868468240669678 26.28462756173175 30.155293162364245 21.691611035234327 42-43 21.919077664301376 26.170299605246477 29.775185659520453 22.135437070931697 44-45 21.697097062381093 26.480479669965185 29.754727817774405 22.06769544987931 46-47 21.99627648322815 26.618373005500523 29.30976767904824 22.07558283222309 48-49 22.057319068551784 26.84510106594168 28.929233365915145 22.168346499591394 50-51 22.061746337388005 27.046723387191836 28.691502284335996 22.200027991084163 52-53 22.26916291795802 26.999389407012742 28.592129224974144 22.139318450055097 54-55 22.007035356977774 27.01390476556763 28.62203723369023 22.357022643764367 56-57 22.06115969293408 27.270364549992916 28.49684097497203 22.17163478210097 58-59 22.164365453455094 26.775374240941513 28.576068237274953 22.48419206832844 60-61 22.091266544869697 26.94222073783897 28.8002951042532 22.16621761303814 62-63 21.989980370985524 27.016496634008618 28.840299876642046 22.15322311836381 64-65 21.954597004349445 26.854566916096502 28.74952152556344 22.44131455399061 66-67 21.93095398665312 27.10691250606207 28.577547868109264 22.384585639175544 68-69 21.927680719844886 26.911731813331546 28.744004023198926 22.416583443624642 70-71 21.89576116188436 26.949448173302642 28.565079654488684 22.589711010324315 72-73 21.939061486206892 27.02552078919086 28.34400824459732 22.69140948000493 74-75 21.72725447019915 27.29570399405208 28.307498710998814 22.669542824749964 76-77 21.8861562165803 27.02874930757587 28.338801525967504 22.746292949876327 78-79 21.92104205434629 27.115435757533252 28.24366578815364 22.71985639996682 80-81 21.851156554520994 27.423217674914387 28.123298883480125 22.60232688708449 82-83 22.01928437619486 27.22625819447144 28.035541239187072 22.718916190146626 84-85 21.870197334483926 27.17022186289933 27.978199970309582 22.981380832307163 86-87 22.07986813249409 26.93956309988323 28.104369001167722 22.876199766454956 88-89 21.97515589005851 26.98949478371991 27.978223116889406 23.057126209332175 90-91 21.95752358949399 27.002016001162993 28.02754702755629 23.012913381786724 92-93 22.232037838249887 26.7176358360542 27.931605895733934 23.11872042996198 94-95 21.93642305131883 26.629165097967 28.043755233698555 23.390656617015615 96-97 22.20082088074435 26.44929187994797 27.975550233713097 23.374337005594583 98-99 22.012880314358302 26.517179806595852 27.753964913180383 23.715974965865456 100-101 22.88296359970762 25.48351906438451 27.762257126758637 23.871260209149238 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3728.0 1 2443.5 2 1757.5 3 2195.5 4 1635.0 5 1418.0 6 2518.0 7 5094.5 8 7869.0 9 8379.0 10 6058.5 11 3816.0 12 3189.5 13 3142.0 14 3143.5 15 3018.5 16 2994.0 17 3044.0 18 3058.5 19 3148.5 20 3343.0 21 3620.0 22 3983.0 23 4537.0 24 5310.5 25 6194.5 26 7111.5 27 8231.0 28 9350.0 29 10831.5 30 13088.5 31 15325.5 32 17898.5 33 20350.0 34 22289.0 35 24365.5 36 27297.0 37 30146.5 38 32010.5 39 33618.5 40 35183.5 41 37731.5 42 39977.0 43 42598.0 44 46011.5 45 49345.5 46 50813.0 47 51485.5 48 53287.5 49 53120.0 50 51866.0 51 50234.0 52 48679.5 53 48221.5 54 47741.0 55 45598.5 56 42203.0 57 39066.0 58 35923.5 59 31927.0 60 27404.0 61 23046.5 62 18925.5 63 14895.0 64 11507.5 65 9166.0 66 7312.0 67 5946.0 68 4595.0 69 3193.5 70 2267.5 71 1686.5 72 1350.5 73 1094.0 74 806.0 75 631.0 76 448.5 77 287.0 78 195.5 79 119.5 80 74.5 81 45.0 82 28.0 83 17.0 84 8.5 85 7.0 86 6.5 87 6.0 88 5.0 89 3.0 90 1.5 91 1.5 92 1.5 93 1.5 94 1.5 95 0.5 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.146071758819296 2 0.05153798897912783 3 0.1566071488316039 4 0.1284178620419152 5 0.19604367631010777 6 0.20074189077505591 7 0.16927809087343365 8 0.07175454819193487 9 0.1230789819681105 10-11 0.10268446008617664 12-13 0.15251400744168697 14-15 0.14062610114401522 16-17 0.13685329255852657 18-19 0.13115848714646824 20-21 0.1325465959656575 22-23 0.1938013466791098 24-25 0.21693649366559675 26-27 0.14774460790908814 28-29 0.12549927426823532 30-31 0.09702524720794369 32-33 0.12236713129160322 34-35 0.09101010899145708 36-37 0.0843543051661139 38-39 0.0519650993850322 40-41 0.0591903837515812 42-43 0.06228693419438791 44-45 0.040824636297693104 46-47 0.05267695006153949 48-49 0.04495337022143539 50-51 0.05484809462488673 52-53 0.029648580676528643 54-55 0.052534579926238036 56-57 0.028474027060291618 58-59 0.0531752455350946 60-61 0.03840434399756832 62-63 0.05303287539979314 64-65 0.04175004217715258 66-67 0.040290748290312636 68-69 0.06627329798282874 70-71 0.05160917404677856 72-73 0.05111087857322345 74-75 0.042782225658088154 76-77 0.0215690754981709 78-79 0.023669034993867404 80-81 0.0270859182411024 82-83 0.023918182730644957 84-85 0.021035187490790433 86-87 0.024843588610104436 88-89 0.06513433690041708 90-91 0.10791656255850522 92-93 0.08043912644532382 94-95 0.07300028687582263 96-97 0.06627329798282874 98-99 0.05595146317347303 100-101 0.1295212305905015 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1404789.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.91967093562211 #Duplication Level Percentage of deduplicated Percentage of total 1 77.50812683605488 42.56720820672595 2 12.575616132256497 13.812973995924555 3 4.118189768192327 6.785088807587054 4 1.848017559603448 4.059700650266911 5 1.0204304743479184 2.8020852931934237 6 0.617135718761064 2.0335734358185764 7 0.41017536145715694 1.576868711398884 8 0.295828708809667 1.2997452272909507 9 0.2168121583284193 1.071652715121491 >10 1.253911952582263 12.7754479637472 >50 0.09145234042923843 3.4446819754125473 >100 0.04170460002112877 4.0334471936040375 >500 0.001299194577950525 0.4288928969400499 >1k 9.094362045653675E-4 1.0462246793931045 >5k 1.2991945779505248E-4 0.508877747625426 >10k+ 2.5983891559010496E-4 1.7535304999498698 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13820 0.9837776349330754 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10756 0.7656665876512415 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7132 0.5076919024849995 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3814 0.2714998480198806 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2464 0.17540000669139635 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2400 0.1708441623617497 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2060 0.14664123936050183 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1442 0.10264886755235128 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 7.118506765072904E-5 0.0 8 0.0 0.0 0.0 7.118506765072904E-5 0.0 9 0.0 0.0 0.0 7.118506765072904E-5 0.0 10-11 0.0 0.0 0.0 7.118506765072904E-5 0.0 12-13 0.0 0.0 0.0 1.423701353014581E-4 0.0 14-15 0.0 0.0 0.0 1.423701353014581E-4 0.0 16-17 0.0 0.0 0.0 2.1355520295218713E-4 0.0 18-19 0.0 0.0 0.0 2.1355520295218713E-4 0.0 20-21 0.0 0.0 0.0 2.1355520295218713E-4 0.0 22-23 0.0 0.0 0.0 6.406656088565614E-4 0.0 24-25 0.0 0.0 0.0 0.0011389610824116647 0.0 26-27 0.0 0.0 0.0 0.003096550442806713 0.0 28-29 0.0 0.0 0.0 0.008933725990166494 0.0 30-31 0.0 0.0 0.0 0.023669034993867407 0.0 32-33 0.0 0.0 0.0 0.047836365461289915 0.0 34-35 0.0 0.0 0.0 0.08253908594102033 0.0 36-37 0.0 0.0 0.0 0.13927358485865138 0.0 38-39 0.0 0.0 0.0 0.22013982170987956 0.0 40-41 0.0 0.0 0.0 0.3094058965438938 0.0 42-43 0.0 0.0 0.0 0.3958601612057042 0.0 44-45 0.0 0.0 0.0 0.49712091993886626 0.0 46-47 0.0 0.0 0.0 0.6151813546376004 0.0 48-49 0.0 0.0 0.0 0.7372993381924261 0.0 50-51 0.0 0.0 0.0 0.8662510882417217 0.0 52-53 0.0 0.0 0.0 0.9939570996071296 0.0 54-55 0.0 0.0 0.0 1.1273579163845957 0.0 56-57 0.0 0.0 0.0 1.2810464774425196 0.0 58-59 0.0 3.559253382536452E-5 0.0 1.446480574662814 0.0 60-61 0.0 7.118506765072904E-5 0.0 1.6225568394968923 0.0 62-63 0.0 7.118506765072904E-5 0.0 1.7950026658807836 0.0 64-65 0.0 7.118506765072904E-5 0.0 1.9688010085500385 0.0 66-67 0.0 7.118506765072904E-5 0.0 2.169009011317714 0.0 68-69 0.0 7.118506765072904E-5 0.0 2.385376024442105 0.0 70-71 0.0 7.118506765072904E-5 0.0 2.612136057443502 0.0 72-73 0.0 7.118506765072904E-5 0.0 2.842455343827436 0.0 74-75 0.0 7.118506765072904E-5 0.0 3.077472844676318 0.0 76-77 0.0 7.118506765072904E-5 0.0 3.3142343796826426 0.0 78-79 0.0 7.118506765072904E-5 0.0 3.579256386546307 0.0 80-81 0.0 7.118506765072904E-5 0.0 3.8676270955994103 0.0 82-83 0.0 7.118506765072904E-5 0.0 4.159699428170351 0.0 84-85 0.0 7.118506765072904E-5 0.0 4.4466464358704405 0.0 86-87 0.0 7.118506765072904E-5 0.0 4.742598354628346 0.0 88-89 0.0 7.118506765072904E-5 0.0 5.055599097088601 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4595 0.0 52.980137 1 GTATCAA 8155 0.0 42.704247 1 TATCAAC 10715 0.0 32.404865 2 ATCAACG 10785 0.0 32.194542 3 TCAACGC 10915 0.0 31.854673 4 CAACGCA 11105 0.0 31.30966 5 AACGCAG 11350 0.0 30.671339 6 GTGGTAT 2105 0.0 28.29017 1 ACGCAGA 12990 0.0 26.715343 7 CGCAGAG 13135 0.0 26.492813 8 TGGTATC 2185 0.0 25.90449 2 GTAAGGT 525 0.0 25.367535 4 GCAGAGT 13810 0.0 24.991365 9 GTACATG 9090 0.0 24.89473 1 TAAGGTG 620 0.0 24.549227 5 TACATGG 9180 0.0 24.352005 2 GAGTACT 8030 0.0 24.0366 12-13 ACATGGG 9210 0.0 23.446377 3 CATGGGG 3875 0.0 22.708035 4 GTACTTT 8590 0.0 22.190508 14-15 >>END_MODULE