FastQCFastQC Report
Thu 26 May 2016
SRR938216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938216_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1404789
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134550.9577950852405592No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108430.7718596885368549No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75940.5405794037396363No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36080.2568357240838304No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26260.18693198765081445No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24480.1742610456089847No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20410.14528872307513796No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47900.055.1412161
GTATCAA84950.042.1083951
ATCAACG110000.032.511063
TATCAAC111200.032.2952462
TCAACGC111950.031.9849134
CAACGCA113400.031.5759355
AACGCAG117150.030.5651826
TATTACG650.00584266729.2326552
TATAACG1004.951706E-528.5018352
CGTATAG856.4041454E-427.9439721
TAGACCG856.4161554E-427.935025
ACGCAGA131400.027.2152927
GTGGTAT21700.027.1455731
CGCAGAG132500.026.8459858
TGGTATC21800.026.366382
GTACATG88200.025.3143251
GCAGAGT139700.025.1224169
TACATGG89050.024.3783252
GAGTACT83450.024.35912712-13
TACGCAT2002.0596417E-823.7447685
Produced by FastQC (version 0.11.5)