##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938216_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1404789 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52097859536201 34.0 31.0 34.0 31.0 34.0 2 32.72842825506179 34.0 31.0 34.0 31.0 34.0 3 32.77059900098876 34.0 31.0 34.0 31.0 34.0 4 36.23766487351481 37.0 35.0 37.0 35.0 37.0 5 36.217253979067316 37.0 37.0 37.0 35.0 37.0 6 36.25850287836821 37.0 37.0 37.0 35.0 37.0 7 36.215471504973344 37.0 37.0 37.0 35.0 37.0 8 36.208585061528815 37.0 37.0 37.0 35.0 37.0 9 37.888106327711846 39.0 38.0 39.0 35.0 39.0 10-11 37.86451915554578 39.0 38.0 39.0 35.0 39.0 12-13 37.77924798670833 39.0 38.0 39.0 35.0 39.0 14-15 39.02489519778415 40.0 38.0 41.0 35.5 41.0 16-17 38.92197796252675 40.0 38.0 41.0 35.5 41.0 18-19 39.19076174429043 40.5 39.0 41.0 36.0 41.0 20-21 39.18391801188648 41.0 39.0 41.0 36.0 41.0 22-23 39.075612778858606 40.0 39.0 41.0 35.0 41.0 24-25 38.97283150708042 40.0 38.0 41.0 35.0 41.0 26-27 38.796158711379434 40.0 38.0 41.0 35.0 41.0 28-29 38.66763727506408 40.0 38.0 41.0 35.0 41.0 30-31 38.391312503158844 40.0 38.0 41.0 34.0 41.0 32-33 38.14470251404303 40.0 37.0 41.0 34.0 41.0 34-35 37.970092661602564 40.0 37.0 41.0 33.0 41.0 36-37 37.956283826254335 40.0 37.0 41.0 33.0 41.0 38-39 37.810965205450785 40.0 37.0 41.0 33.0 41.0 40-41 37.61929798709984 40.0 37.0 41.0 33.0 41.0 42-43 37.36265019159461 40.0 36.0 41.0 33.0 41.0 44-45 37.034625840606665 40.0 36.0 41.0 31.5 41.0 46-47 36.56490583283326 39.5 35.0 41.0 30.5 41.0 48-49 36.12804200488472 39.0 35.0 41.0 30.0 41.0 50-51 35.74560450003524 39.0 35.0 40.5 28.5 41.0 52-53 35.248584306967096 38.0 35.0 40.0 27.0 41.0 54-55 34.941548161325294 38.0 34.5 40.0 26.5 41.0 56-57 34.61040839585162 38.0 34.0 40.0 26.0 41.0 58-59 34.182654121010344 37.5 33.5 40.0 25.0 41.0 60-61 33.72125813912267 37.0 33.0 40.0 23.0 41.0 62-63 33.268879881604995 36.0 33.0 39.0 22.0 41.0 64-65 32.78762860472284 35.5 32.0 39.0 21.0 40.0 66-67 32.24986457040879 35.0 31.5 38.5 19.5 40.0 68-69 31.697938266885632 35.0 31.0 38.0 17.0 40.0 70-71 31.127951243923462 35.0 30.5 37.0 11.5 39.5 72-73 30.565151421316656 34.5 30.0 36.5 7.5 39.0 74-75 29.990096377463093 34.0 29.0 36.0 7.0 39.0 76-77 28.648026856702323 32.5 28.0 35.0 2.0 36.5 78-79 29.354998508672832 34.0 29.0 35.0 2.0 37.0 80-81 29.036899491667434 34.0 29.0 35.0 2.0 37.0 82-83 28.507058711308247 34.0 28.5 35.0 2.0 36.0 84-85 27.934917984124304 33.0 27.0 35.0 2.0 36.0 86-87 27.307179939478456 33.0 26.0 35.0 2.0 35.5 88-89 26.599801464846323 32.5 25.0 35.0 2.0 35.0 90-91 26.089694253015935 32.0 24.0 34.5 2.0 35.0 92-93 25.2935764730504 32.0 20.5 34.0 2.0 35.0 94-95 24.493049489994583 31.0 16.5 34.0 2.0 35.0 96-97 23.473552611815727 31.0 2.0 34.0 2.0 35.0 98-99 22.076242410781976 30.5 2.0 34.0 2.0 35.0 100-101 17.757448271590967 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1281363951141401 1101 2 0.1307461865238011 1101 3 0.12921587394075118 1101 4 0.005076945893748075 1101 5 0.11848834667805619 1101 6 0.09824428445959654 1101 7 0.12126575164343478 1101 8 0.16711277420321125 1101 9 -0.008128223430773573 1101 10-11 0.1733421046984418 1101 12-13 0.25962205864323806 1101 14-15 0.20279374340488943 1101 16-17 0.25079308123314803 1101 18-19 0.20369238506229692 1101 20-21 0.22348537480018393 1101 22-23 0.26766638694603273 1101 24-25 0.2243599685259312 1101 26-27 0.2854336947360281 1101 28-29 0.21224033376098106 1101 30-31 0.2503237785831729 1101 32-33 0.3839728945474903 1101 34-35 0.3547020931261997 1101 36-37 0.36197445329626277 1101 38-39 0.3516683089753556 1101 40-41 0.4138890286585024 1101 42-43 0.4053338317361579 1101 44-45 0.43885792413081504 1101 46-47 0.5617318988710096 1101 48-49 0.6540296217928301 1101 50-51 0.4982310640666654 1101 52-53 0.4123733915905632 1101 54-55 0.2738873086959259 1101 56-57 0.3943834109013906 1101 58-59 0.5153906463968454 1101 60-61 0.4594951325502876 1101 62-63 0.38314783543038544 1101 64-65 0.3441998566029767 1101 66-67 -0.07002744808868755 1101 68-69 0.0869018068902605 1101 70-71 0.250809982353676 1101 72-73 0.2623518546364849 1101 74-75 0.1423807080558035 1101 76-77 0.1702035014308052 1101 78-79 0.038836906150304884 1101 80-81 0.02544508853214822 1101 82-83 0.08325623442333452 1101 84-85 -0.2236724533701171 1101 86-87 -0.05831791030448841 1101 88-89 -0.14468887356883897 1101 90-91 -0.28418526002056055 1101 92-93 -0.23320693713728247 1101 94-95 -0.5428886098935131 1101 96-97 -0.32854945667805424 1101 98-99 -0.18346372513093456 1101 100-101 -0.6796761547745263 1102 1 0.08806602822236442 1102 2 0.06368892397478731 1102 3 0.020937328388626497 1102 4 0.02258518269622556 1102 5 0.01809029667581541 1102 6 -0.0444424785142985 1102 7 0.013288352534949865 1102 8 0.09456629776568093 1102 9 0.049504049926710536 1102 10-11 0.04836334894013561 1102 12-13 0.11048748196270708 1102 14-15 0.09249364053803788 1102 16-17 -0.08906558865369618 1102 18-19 0.05625585689318058 1102 20-21 0.025186404959555375 1102 22-23 0.11303663308107303 1102 24-25 0.20302478657085743 1102 26-27 0.254417101865144 1102 28-29 0.06966985505740553 1102 30-31 -0.021140359646025786 1102 32-33 -0.042371455975036554 1102 34-35 0.007009143232785675 1102 36-37 0.06488006923019185 1102 38-39 0.028989894615733647 1102 40-41 0.056726364854213784 1102 42-43 -0.08742970867263011 1102 44-45 -0.07289424129101008 1102 46-47 -0.10805655320645258 1102 48-49 0.008238963295880808 1102 50-51 0.18347339750435054 1102 52-53 0.04372541985658529 1102 54-55 -0.08298166278461139 1102 56-57 -0.13379316229060834 1102 58-59 0.019697636870482427 1102 60-61 -0.3456586459430895 1102 62-63 -0.17963970724445488 1102 64-65 -0.05384418233734323 1102 66-67 -0.3039049321741416 1102 68-69 -0.24315469533852152 1102 70-71 -0.24487855470075104 1102 72-73 -0.18814086268838537 1102 74-75 -0.105495613186946 1102 76-77 -0.15507938241909613 1102 78-79 -0.15022939079201691 1102 80-81 -0.2655384080464067 1102 82-83 -0.19735559973740635 1102 84-85 -0.6118263660376471 1102 86-87 -0.5633867118310896 1102 88-89 -0.7501646700415492 1102 90-91 -0.862065904652848 1102 92-93 -0.885610890802468 1102 94-95 -0.7725023374007058 1102 96-97 -0.7919333289442569 1102 98-99 -0.8560817611194231 1102 100-101 -1.3971038870821744 1103 1 0.10846711999177217 1103 2 0.12998182150921878 1103 3 0.105812459392439 1103 4 0.07365692876042829 1103 5 0.04920572487721131 1103 6 0.027777329545592977 1103 7 -0.031524180659786794 1103 8 0.052543400146049635 1103 9 0.02380088964621052 1103 10-11 0.10661765395959577 1103 12-13 0.17727514076488404 1103 14-15 0.16920350650942595 1103 16-17 0.1704744708198973 1103 18-19 0.13742482269444167 1103 20-21 0.14194608788063334 1103 22-23 0.11804900211276248 1103 24-25 0.19886277071691438 1103 26-27 0.14212361925137174 1103 28-29 0.12715929664745573 1103 30-31 0.0038936516409009414 1103 32-33 -0.061775072674223 1103 34-35 -0.10086926251121753 1103 36-37 -0.21805975564362967 1103 38-39 -0.20467346890882254 1103 40-41 -0.11428835017522232 1103 42-43 -0.15714248015937926 1103 44-45 -0.3109397354700434 1103 46-47 -0.2858118934755325 1103 48-49 -0.5053638473571098 1103 50-51 -0.613650341095834 1103 52-53 -0.4727814860677739 1103 54-55 -0.5031214569843669 1103 56-57 -0.7831298356885341 1103 58-59 -0.727836935354155 1103 60-61 -0.9048652669962891 1103 62-63 -0.6113671966008383 1103 64-65 -0.6928882876629885 1103 66-67 -0.648583692373979 1103 68-69 -0.7896852600536945 1103 70-71 -0.9106394844656727 1103 72-73 -0.9408783807590986 1103 74-75 -1.0311786081115244 1103 76-77 -0.985082875853994 1103 78-79 -1.0248701776385225 1103 80-81 -0.8731072329741956 1103 82-83 -0.92470152647752 1103 84-85 -0.8446737426952531 1103 86-87 -0.9835716650673483 1103 88-89 -1.1799497527905132 1103 90-91 -1.4316497314693635 1103 92-93 -1.17074504722315 1103 94-95 -1.144494930182642 1103 96-97 -1.4420342977333966 1103 98-99 -1.5057416482502362 1103 100-101 -1.5662426559729923 1104 1 0.1123672252327097 1104 2 0.11179085445137815 1104 3 0.10269174026191763 1104 4 0.10651866206646332 1104 5 -0.05404044737399971 1104 6 0.012022936173075038 1104 7 0.007717618986660568 1104 8 4.4305246470344173E-4 1104 9 0.1016638372707277 1104 10-11 0.037464729727254564 1104 12-13 0.031214056576899907 1104 14-15 0.054569989943637154 1104 16-17 0.13852307953897025 1104 18-19 0.13927619540343983 1104 20-21 0.12246831361265009 1104 22-23 0.10079086445932006 1104 24-25 0.16141493482293612 1104 26-27 0.1469864488832897 1104 28-29 0.08360653411259733 1104 30-31 -0.05082687256372509 1104 32-33 0.0701967424382488 1104 34-35 0.10767287275275805 1104 36-37 0.03727253110061923 1104 38-39 0.10659127950502523 1104 40-41 0.20984605768445874 1104 42-43 0.06826942248984125 1104 44-45 -0.11399870690260627 1104 46-47 0.0390132737644322 1104 48-49 0.04033262381751257 1104 50-51 0.2709642701979149 1104 52-53 0.22415319312198534 1104 54-55 0.2630084756222786 1104 56-57 0.3876250365328886 1104 58-59 0.31638965488716764 1104 60-61 0.19217967971920302 1104 62-63 0.04207794927346953 1104 64-65 -0.01161408396754382 1104 66-67 0.038167643244072735 1104 68-69 0.15007263817530614 1104 70-71 -0.0012003937812146148 1104 72-73 -0.20258703004011025 1104 74-75 -0.28493968843697104 1104 76-77 -0.12527554771974536 1104 78-79 -0.09509799860088819 1104 80-81 -0.24076054002110325 1104 82-83 -0.3889428896042517 1104 84-85 -0.37794580453396165 1104 86-87 -0.3026903291159506 1104 88-89 -0.3818382693589477 1104 90-91 -0.5888095278448198 1104 92-93 -0.6530824012998977 1104 94-95 -0.4306447151768431 1104 96-97 -0.7684870221190785 1104 98-99 -0.7520903592471981 1104 100-101 -1.0006686642938547 1105 1 0.030940862270874447 1105 2 0.12752515453041013 1105 3 0.11947479219024615 1105 4 0.05659313755201367 1105 5 0.0349820625077939 1105 6 0.030805265762232636 1105 7 0.010743988396150428 1105 8 0.04527593471384961 1105 9 0.016985940597464833 1105 10-11 0.15270553211812654 1105 12-13 -0.006359669620287889 1105 14-15 0.07039093042520506 1105 16-17 0.016493346769209438 1105 18-19 0.007523847927060956 1105 20-21 0.07235691521136545 1105 22-23 -0.036233450766680164 1105 24-25 -0.034750774013936336 1105 26-27 0.07968499015106545 1105 28-29 0.06780876490147136 1105 30-31 0.04671007176024489 1105 32-33 0.1529892029098221 1105 34-35 0.1045402177331809 1105 36-37 0.14724338063495424 1105 38-39 0.19060325401460432 1105 40-41 0.09332302770839362 1105 42-43 0.06576264363393136 1105 44-45 -0.04873657183122049 1105 46-47 -0.13466043545728468 1105 48-49 -0.08153491005914049 1105 50-51 -0.04200025710565569 1105 52-53 -0.008914526930780653 1105 54-55 0.14621444252664872 1105 56-57 0.05134542579008183 1105 58-59 0.06641118905698562 1105 60-61 -0.07147839896941832 1105 62-63 0.019711628614317078 1105 64-65 -0.015541362446420237 1105 66-67 0.014808987741922408 1105 68-69 0.01041863561538392 1105 70-71 -0.25714017265677214 1105 72-73 -0.2325625944242553 1105 74-75 -0.34947444710567055 1105 76-77 -0.43209817718543775 1105 78-79 -0.5134764428717169 1105 80-81 -0.5124807096549553 1105 82-83 -0.430935528192375 1105 84-85 -0.3966221003746675 1105 86-87 -0.5584291224065616 1105 88-89 -0.9419810648316833 1105 90-91 -0.7932791147967784 1105 92-93 -0.8634505877500445 1105 94-95 -0.8849937863363166 1105 96-97 -1.1218630006645007 1105 98-99 -1.483364320733525 1105 100-101 -2.1911123286388587 1106 1 0.12395311869073566 1106 2 0.14668837662941314 1106 3 0.19118363380734849 1106 4 0.15483633603112423 1106 5 0.140576808521935 1106 6 0.14283095959657288 1106 7 0.12349556185545651 1106 8 0.14345518665457035 1106 9 0.14972455192061318 1106 10-11 0.15562114861172205 1106 12-13 0.20603990983040177 1106 14-15 0.19081407215896462 1106 16-17 0.23287201397356228 1106 18-19 0.1965201465170736 1106 20-21 0.25029391922962674 1106 22-23 0.27695249827772983 1106 24-25 0.14928016228436292 1106 26-27 0.13558060174785425 1106 28-29 0.08216965957691258 1106 30-31 0.028513248923836443 1106 32-33 0.15306524744196537 1106 34-35 -0.060630234357326174 1106 36-37 -0.08608716823950857 1106 38-39 0.03394258833689889 1106 40-41 -0.06383192742821109 1106 42-43 -0.1626912615696483 1106 44-45 -0.21460352270472782 1106 46-47 -0.08706663999978304 1106 48-49 -0.22370880431691376 1106 50-51 -0.23950259749135228 1106 52-53 -0.309049621194319 1106 54-55 -0.34395593755198917 1106 56-57 -0.3657706429380383 1106 58-59 -0.07470067417807513 1106 60-61 -0.37546238353007766 1106 62-63 -0.3410862628781999 1106 64-65 -0.5065410311918228 1106 66-67 -0.47237001935844347 1106 68-69 -0.6578563921091849 1106 70-71 -1.0764384890193064 1106 72-73 -0.8179101957089472 1106 74-75 -0.5699165999724656 1106 76-77 -0.5494026289590899 1106 78-79 -0.6593716223973018 1106 80-81 -0.6229627570963707 1106 82-83 -0.4876863527282609 1106 84-85 -0.5929601528857198 1106 86-87 -0.7690572099153812 1106 88-89 -1.0067805117805406 1106 90-91 -1.112836097787575 1106 92-93 -1.3008302199134256 1106 94-95 -1.3155924930031766 1106 96-97 -1.5423063057211728 1106 98-99 -1.354647716237107 1106 100-101 -1.7762234429504957 1107 1 0.059966426598386136 1107 2 0.022655431443389773 1107 3 0.037765097406705195 1107 4 0.01190291614229011 1107 5 -0.015784614775014916 1107 6 0.046084093852265084 1107 7 0.054070479695354834 1107 8 -0.01177344157604665 1107 9 0.010926320898583697 1107 10-11 0.02802103500763309 1107 12-13 -0.07738985173624968 1107 14-15 0.008727273224671706 1107 16-17 0.01886841651071336 1107 18-19 0.024346927328807055 1107 20-21 0.007290713971379148 1107 22-23 0.11414187900019357 1107 24-25 0.11293848446015176 1107 26-27 0.09150491978520847 1107 28-29 0.06001361624506529 1107 30-31 -0.0038311878568393354 1107 32-33 0.05339037152737802 1107 34-35 0.01906249362850332 1107 36-37 0.06003176442302305 1107 38-39 0.004572414820181336 1107 40-41 -0.08696550690374494 1107 42-43 -0.07295106330346357 1107 44-45 -0.043361857052381936 1107 46-47 -0.008465593219249001 1107 48-49 0.00984389310400502 1107 50-51 0.013474235388919453 1107 52-53 -0.22099887803423712 1107 54-55 -0.04214773912351433 1107 56-57 -0.07886297330955472 1107 58-59 0.03318453159409529 1107 60-61 -0.2570904383305219 1107 62-63 -0.16595933735650448 1107 64-65 -0.3301617558236316 1107 66-67 -0.4110833177990898 1107 68-69 -0.22841021204670398 1107 70-71 -0.47270470126961683 1107 72-73 -0.5738320793729486 1107 74-75 -0.5123312278514476 1107 76-77 -0.6077518533406945 1107 78-79 -0.5468443371293183 1107 80-81 -0.47290798369595777 1107 82-83 -0.45572492402158815 1107 84-85 -0.5451155200027458 1107 86-87 -0.7966118818835746 1107 88-89 -0.6700263429161772 1107 90-91 -0.7792174018002349 1107 92-93 -0.6628531841687106 1107 94-95 -1.1530504662521146 1107 96-97 -1.4984094149569138 1107 98-99 -1.5660914348776451 1107 100-101 -2.0563651775767546 1108 1 -0.017839760446555886 1108 2 0.0031370096423870564 1108 3 -0.05571755122577571 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-0.1062837608565701 2316 54-55 -0.18521563631967553 2316 56-57 -0.2958951783752255 2316 58-59 -0.34423317727516434 2316 60-61 -0.06648539976532675 2316 62-63 -0.15111951579598326 2316 64-65 0.053295964309249655 2316 66-67 -0.03548536134187685 2316 68-69 0.13665752847502333 2316 70-71 0.26567926543071607 2316 72-73 0.5649065220324623 2316 74-75 0.7722846127810072 2316 76-77 0.7169010810233019 2316 78-79 0.7380437782677554 2316 80-81 0.7303853553115296 2316 82-83 0.8818005091091266 2316 84-85 0.7158981636038142 2316 86-87 0.9620610009472408 2316 88-89 1.0635904864590096 2316 90-91 1.2401729652625662 2316 92-93 1.2042242235892147 2316 94-95 1.60117349773817 2316 96-97 1.8337359450449462 2316 98-99 2.179647126300548 2316 100-101 2.651055685953459 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 17.0 5 33.0 6 62.0 7 190.0 8 373.0 9 702.0 10 1133.0 11 1931.0 12 2973.0 13 4673.0 14 5787.0 15 8397.0 16 11558.0 17 16858.0 18 17182.0 19 11876.0 20 10383.0 21 10995.0 22 11825.0 23 12949.0 24 14668.0 25 16873.0 26 19167.0 27 23084.0 28 27446.0 29 33135.0 30 40213.0 31 49397.0 32 61814.0 33 79252.0 34 110893.0 35 164026.0 36 215604.0 37 267171.0 38 145346.0 39 6793.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.32585513515361 18.824826773150246 14.814250555828062 22.035067535868087 2 16.057807451833057 20.45409563190502 39.6453352356624 23.84276168059952 3 19.289277944208404 25.255934549099567 29.653523709719675 25.801263796972357 4 12.95989145693158 17.107848301665445 35.839571522535785 34.09268871886719 5 13.602310973422096 37.81051213859007 33.10221700826324 15.484959879724597 6 31.726092849495437 35.84570548304062 16.994051686826577 15.434149980637372 7 28.65441002171856 33.78706695453908 19.63291284313872 17.925610180603634 8 27.36994666102881 34.24336323818025 18.890239032338666 19.496451068452274 9 27.428318416502407 16.569392271721945 19.73933451927656 36.26295479249909 10-11 25.60057061950229 26.915536781680384 25.72293775079389 21.760954848023438 12-13 26.615548287505824 24.467878743873143 26.907622803167904 22.008950165453133 14-15 23.328200889955717 25.39014755952673 26.049570433709263 25.232081116808292 16-17 21.904043253324886 28.94990024897713 26.50810910857645 22.637947389121532 18-19 21.796227049044376 26.58182118453376 30.134810281116952 21.48714148530491 20-21 22.766016818184085 25.53497357966214 30.41307271056365 21.285936891590122 22-23 22.507757392747237 25.555368101544072 30.449056762261094 21.487817743447593 24-25 22.09177499038968 25.893510542876264 30.462185173056938 21.55252929367712 26-27 21.675242666256555 26.277375381007207 30.434260993762198 21.61312095897404 28-29 21.63342278338813 26.291446289566878 30.582308110411194 21.492822816633794 30-31 21.701353233483765 26.078797847156054 30.46937650316267 21.750472416197514 32-33 21.480272126276613 26.329470119711928 30.30209519009616 21.88816256391529 34-35 21.979340196204063 26.34644682699016 30.183567553714063 21.49064542309171 36-37 22.02302076025671 26.046581820369163 30.066868335901358 21.86352908347277 38-39 21.655717463211282 26.231531783893796 30.292875323590874 21.819875429304048 40-41 21.832798423353278 26.241685818899903 30.239682620473985 21.68583313727284 42-43 21.872489525638027 26.180227721937054 29.881518957785413 22.065763794639505 44-45 21.673120188555075 26.431096211280202 29.835914801409608 22.059868798755115 46-47 21.947443221758768 26.656667512584665 29.344729141922375 22.051160123734192 48-49 21.957175637971517 26.796885928055158 29.062144407202673 22.183794026770652 50-51 22.03506506403537 26.98743496532967 28.76385985506839 22.21364011556657 52-53 22.247427619713083 26.99672488033764 28.590894092361864 22.164953407587415 54-55 21.953968163567502 26.98333428254122 28.638266366716973 22.424431187174303 56-57 21.990376266821688 27.251105960385136 28.51959188308556 22.238925889707616 58-59 22.097191471849033 26.855444587204495 28.577429884699008 22.46993405624746 60-61 22.049093852376743 27.005818878680582 28.754863896327997 22.19022337261468 62-63 21.94775156879505 27.123217739532496 28.76237583227928 22.166654859393173 64-65 21.86626016028617 26.931913978617118 28.6711598817505 22.53066597934621 66-67 21.83294176277944 27.210415776733587 28.475651431012388 22.480991029474584 68-69 21.874124370303196 26.97614157189839 28.616192656157153 22.533541401641262 70-71 21.758126043922715 27.130207510120623 28.471305563743183 22.64036088221348 72-73 21.921889855035325 27.159048964510045 28.164764332289355 22.754296848165275 74-75 21.75684450197144 27.505806097007028 28.038927548737753 22.69842185228378 76-77 21.798385687133166 27.193158269659957 28.33100213350174 22.677453909705136 78-79 21.77885169957975 27.234467810590385 28.26511706728681 22.721563422543053 80-81 21.691822316545775 27.515562021512558 28.167232240144408 22.62538342179726 82-83 21.92340161297616 27.45431259806943 28.006510011938346 22.61577577701607 84-85 21.768524151984682 27.352336699133232 27.91959674482305 22.95954240405904 86-87 21.895189572362916 27.213505228416096 28.053861408699433 22.83744379052156 88-89 21.824313547905682 27.267976201664972 27.850972752672188 23.056737497757158 90-91 21.724372295744004 27.26160624929017 28.063132328171044 22.95088912679478 92-93 21.90415295426097 27.057252468233028 27.96284086394208 23.075753713563923 94-95 21.651900056707486 26.98852643483641 28.047137339974004 23.3124361684821 96-97 21.842058423574475 26.91193746128708 27.915411834226845 23.330592280911596 98-99 21.67296549863291 26.966226940917704 27.77046266068785 23.590344899761536 100-101 22.0851527490486 26.518066366447503 27.71538340237235 23.68139748213155 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3600.0 1 2303.5 2 1669.5 3 2179.5 4 1596.0 5 1375.5 6 2567.0 7 5329.0 8 7973.5 9 8340.5 10 6014.0 11 3650.5 12 3112.5 13 3077.0 14 3100.0 15 3072.0 16 2929.5 17 2885.5 18 2983.5 19 3133.0 20 3223.5 21 3494.0 22 3943.5 23 4459.5 24 5134.0 25 6030.5 26 7007.0 27 8062.5 28 9277.5 29 10809.5 30 13100.5 31 15456.0 32 18078.5 33 20469.5 34 22291.5 35 24464.0 36 27449.5 37 30512.5 38 32489.0 39 33789.5 40 35312.5 41 37991.5 42 40374.5 43 43239.0 44 46702.5 45 49846.5 46 51170.0 47 51778.0 48 53809.5 49 53527.0 50 52131.5 51 50617.5 52 48874.5 53 48537.0 54 48477.5 55 46355.0 56 42621.0 57 39215.5 58 36017.0 59 32072.5 60 27169.0 61 22519.5 62 18222.5 63 13980.5 64 10824.0 65 8534.5 66 6617.5 67 5409.5 68 4069.5 69 2742.0 70 1928.5 71 1399.0 72 1098.5 73 895.0 74 694.0 75 537.0 76 368.0 77 200.5 78 114.0 79 74.0 80 40.0 81 19.0 82 14.0 83 7.5 84 2.5 85 1.5 86 1.0 87 1.0 88 0.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009111688659293318 2 0.013881088191892163 3 0.009467613997546963 4 7.118506765072904E-5 5 9.254058794594776E-4 6 0.0014948864206653098 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.474432103326549E-4 14-15 0.0 16-17 0.002954180307505255 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003488068314885723 26-27 0.020501299483409965 28-29 0.006726988892993895 30-31 0.0030253653751559843 32-33 0.0 34-35 0.009182873726944046 36-37 9.609984132848421E-4 38-39 0.0031677355104574425 40-41 0.002527069901600881 42-43 0.0035236608487110876 44-45 3.559253382536452E-5 46-47 2.4914773677755164E-4 48-49 3.203328044282807E-4 50-51 0.004093141389916921 52-53 0.007652394772453372 54-55 0.007759172373929466 56-57 0.017120008770000333 58-59 0.023811405129168864 60-61 0.003630438450187181 62-63 0.0204657069495846 64-65 0.021426705362869444 66-67 0.01345397778598779 68-69 0.0314993924354476 70-71 0.01644375062731841 72-73 0.00480499206642421 74-75 0.01644375062731841 76-77 0.020679262152536786 78-79 0.027263880910229223 80-81 0.011923498831497114 82-83 0.034916275682682595 84-85 0.022672444046757202 86-87 0.031606170036923696 88-89 0.022850406715884022 90-91 0.028865544932370625 92-93 0.02441647820420006 94-95 0.015126826875779922 96-97 0.015589529815509661 98-99 0.011638758560894198 100-101 0.010570982546133263 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1404789.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.80780370829245 #Duplication Level Percentage of deduplicated Percentage of total 1 75.59461765591999 39.91985730577242 2 13.546060038670966 14.306753590857616 3 4.4939811797519305 7.119518280273013 4 2.0547306930017872 4.340232604377684 5 1.103450126430918 2.913538883922721 6 0.678692087871514 2.150414313281405 7 0.4666328426389402 1.724929889054377 8 0.3232694672746905 1.3656920458180901 9 0.23900144742280616 1.1359027369351193 >10 1.3462853069070975 13.137489523744733 >50 0.10537842428983567 3.8345346118613435 >100 0.04506718943653843 4.277747104839555 >500 0.0014842354387279192 0.4691352209411538 >1k 9.445134610086759E-4 1.031826721514102 >5k 1.3493049442981085E-4 0.541101589888688 >10k+ 2.698609888596217E-4 1.7313255769180063 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13455 0.9577950852405592 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10843 0.7718596885368549 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7594 0.5405794037396363 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3608 0.2568357240838304 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2626 0.18693198765081445 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2448 0.1742610456089847 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2041 0.14528872307513796 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.118506765072904E-5 0.0 14-15 0.0 0.0 0.0 7.118506765072904E-5 0.0 16-17 0.0 0.0 0.0 1.423701353014581E-4 0.0 18-19 0.0 0.0 0.0 1.423701353014581E-4 0.0 20-21 0.0 0.0 0.0 2.4914773677755164E-4 0.0 22-23 0.0 0.0 0.0 7.118506765072905E-4 0.0 24-25 0.0 0.0 0.0 0.0012101461500623938 0.0 26-27 0.0 0.0 0.0 0.0031677355104574425 0.0 28-29 0.0 0.0 0.0 0.009004911057817224 0.0 30-31 0.0 0.0 0.0 0.023740220061518137 0.0 32-33 0.0 0.0 0.0 0.04801432813041674 0.0 34-35 0.0 0.0 0.0 0.08303738141457542 0.0 36-37 0.0 0.0 0.0 0.14009221313663475 0.0 38-39 0.0 0.0 0.0 0.22152793052906877 0.0 40-41 0.0 0.0 0.0 0.31082959789690834 0.0 42-43 0.0 0.0 0.0 0.39689234468663975 0.0 44-45 0.0 0.0 0.0 0.4985446212918808 0.0 46-47 0.0 0.0 0.0 0.6171033514641701 0.0 48-49 0.0 0.0 0.0 0.7396128528910748 0.0 50-51 0.0 0.0 0.0 0.8693832312183538 0.0 52-53 0.0 0.0 0.0 0.9974807604558407 0.0 54-55 0.0 0.0 0.0 1.1312375025715604 0.0 56-57 0.0 0.0 0.0 1.2871328007266571 0.0 58-59 0.0 0.0 0.0 1.4545600798411717 0.0 60-61 0.0 0.0 0.0 1.6316685281561858 0.0 62-63 0.0 0.0 0.0 1.8056448334945676 0.0 64-65 0.0 0.0 0.0 1.9804041745771075 0.0 66-67 0.0 0.0 0.0 2.1830324696449077 0.0 68-69 0.0 0.0 0.0 2.401214701994392 0.0 70-71 0.0 0.0 0.0 2.62950521395028 0.0 72-73 0.0 0.0 0.0 2.861782089694609 0.0 74-75 0.0 0.0 0.0 3.098543624700934 0.0 76-77 0.0 0.0 0.0 3.3380457848118112 0.0 78-79 0.0 0.0 0.0 3.606377897321235 0.0 80-81 0.0 0.0 0.0 3.897667194148018 0.0 82-83 0.0 0.0 0.0 4.193405557702972 0.0 84-85 0.0 0.0 0.0 4.486652443890151 0.0 86-87 0.0 0.0 0.0 4.787053429376227 0.0 88-89 0.0 0.0 0.0 5.106069310052969 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4790 0.0 55.141216 1 GTATCAA 8495 0.0 42.108395 1 ATCAACG 11000 0.0 32.51106 3 TATCAAC 11120 0.0 32.295246 2 TCAACGC 11195 0.0 31.984913 4 CAACGCA 11340 0.0 31.575935 5 AACGCAG 11715 0.0 30.565182 6 TATTACG 65 0.005842667 29.232655 2 TATAACG 100 4.951706E-5 28.501835 2 CGTATAG 85 6.4041454E-4 27.943972 1 TAGACCG 85 6.4161554E-4 27.93502 5 ACGCAGA 13140 0.0 27.215292 7 GTGGTAT 2170 0.0 27.145573 1 CGCAGAG 13250 0.0 26.845985 8 TGGTATC 2180 0.0 26.36638 2 GTACATG 8820 0.0 25.314325 1 GCAGAGT 13970 0.0 25.122416 9 TACATGG 8905 0.0 24.378325 2 GAGTACT 8345 0.0 24.359127 12-13 TACGCAT 200 2.0596417E-8 23.744768 5 >>END_MODULE