##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938215_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 832412 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06312499099004 33.0 31.0 34.0 31.0 34.0 2 32.21084511035401 34.0 31.0 34.0 31.0 34.0 3 32.2650754674368 34.0 31.0 34.0 30.0 34.0 4 35.79566488709918 37.0 35.0 37.0 35.0 37.0 5 35.83364487777687 37.0 35.0 37.0 35.0 37.0 6 35.908416745553886 37.0 37.0 37.0 35.0 37.0 7 35.85924037615988 37.0 37.0 37.0 35.0 37.0 8 35.84999735707799 37.0 37.0 37.0 35.0 37.0 9 37.51233764049533 39.0 38.0 39.0 35.0 39.0 10-11 37.45029384487489 39.0 38.0 39.0 35.0 39.0 12-13 37.37614306377131 39.0 37.5 39.0 35.0 39.0 14-15 38.55999372906686 40.0 38.0 41.0 34.5 41.0 16-17 38.433914335689536 40.0 38.0 41.0 34.0 41.0 18-19 38.68264813577892 40.0 38.5 41.0 35.0 41.0 20-21 38.72266377707193 40.0 39.0 41.0 35.0 41.0 22-23 38.61215840232961 40.0 38.0 41.0 35.0 41.0 24-25 38.50744222812742 40.0 38.0 41.0 35.0 41.0 26-27 38.30430724208685 40.0 38.0 41.0 34.0 41.0 28-29 38.1239085933408 40.0 38.0 41.0 34.0 41.0 30-31 37.83991280760008 40.0 37.0 41.0 34.0 41.0 32-33 37.468460329740566 40.0 36.0 41.0 33.0 41.0 34-35 37.207859809805726 39.5 35.0 41.0 33.0 41.0 36-37 36.9315591317761 39.0 35.0 41.0 33.0 41.0 38-39 36.64730626180305 39.0 35.0 41.0 31.5 41.0 40-41 36.48754883399086 39.0 35.0 41.0 31.0 41.0 42-43 36.21703435318088 39.0 35.0 41.0 30.5 41.0 44-45 35.82552630187936 39.0 35.0 41.0 29.5 41.0 46-47 35.32430755443218 39.0 35.0 40.5 27.0 41.0 48-49 34.86488962196604 38.5 35.0 40.0 24.0 41.0 50-51 34.100229814082454 37.5 34.0 39.5 23.0 40.5 52-53 34.166985218857974 38.0 34.5 40.0 23.0 40.5 54-55 34.28246229030817 38.0 35.0 40.0 22.0 41.0 56-57 33.96032253259203 38.0 34.0 40.0 18.5 41.0 58-59 33.6598499300827 38.0 34.0 40.0 15.5 41.0 60-61 33.26786254883399 38.0 33.5 40.0 8.5 41.0 62-63 32.76815086759922 37.0 33.0 40.0 4.5 41.0 64-65 32.32423487407678 37.0 32.0 39.5 2.0 41.0 66-67 31.74338008101757 36.0 31.0 39.0 2.0 40.5 68-69 31.1612741046501 35.5 31.0 39.0 2.0 40.0 70-71 30.48297417624926 35.0 29.5 38.0 2.0 40.0 72-73 29.747582327020755 35.0 29.0 37.5 2.0 39.5 74-75 28.977601836590534 34.5 28.0 37.0 2.0 39.0 76-77 28.197936838969163 34.0 26.0 36.0 2.0 39.0 78-79 27.40810920553764 34.0 25.5 36.0 2.0 38.0 80-81 26.627039254599886 33.0 23.0 35.0 2.0 37.0 82-83 25.81957612336199 32.5 20.0 35.0 2.0 37.0 84-85 24.995913682167004 32.0 14.5 35.0 2.0 36.0 86-87 24.26709730277795 31.5 4.5 35.0 2.0 36.0 88-89 23.426857133246518 31.0 2.0 34.5 2.0 35.0 90-91 22.56822643114227 30.5 2.0 34.0 2.0 35.0 92-93 21.683394761248035 29.5 2.0 34.0 2.0 35.0 94-95 20.758595503188324 29.0 2.0 34.0 2.0 35.0 96-97 19.64053797878935 28.0 2.0 34.0 2.0 35.0 98-99 18.3343903019178 25.5 2.0 34.0 2.0 35.0 100-101 14.934245301605454 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6149433444468926 1101 2 0.47727764370774395 1101 3 0.5297057050996798 1101 4 0.4192116412993272 1101 5 0.37830204749721474 1101 6 0.47099261984453733 1101 7 0.47998748506052635 1101 8 0.33614567833971876 1101 9 0.48293172911082394 1101 10-11 0.5460681633637421 1101 12-13 0.5704624452106444 1101 14-15 0.7207747190143792 1101 16-17 0.8666568167945172 1101 18-19 0.7029795156542917 1101 20-21 0.5188447480152902 1101 22-23 0.7958889334323871 1101 24-25 0.9321840641546899 1101 26-27 0.9432122407239234 1101 28-29 0.8817702190931982 1101 30-31 0.8527560645845043 1101 32-33 0.7757439870534455 1101 34-35 0.8449579931793849 1101 36-37 0.9003359268765649 1101 38-39 0.9286560014233416 1101 40-41 0.926930229249102 1101 42-43 0.9187275667577595 1101 44-45 0.9829701533712623 1101 46-47 0.8221501750874793 1101 48-49 0.8659354973717512 1101 50-51 1.047377330170569 1101 52-53 1.1274918162412249 1101 54-55 1.0421548264470388 1101 56-57 1.2524062532387248 1101 58-59 1.4170955548988147 1101 60-61 1.5000936655543882 1101 62-63 1.6240217948828573 1101 64-65 1.437525767389836 1101 66-67 1.7105513642934973 1101 68-69 1.8513233256031434 1101 70-71 2.043408149470853 1101 72-73 2.4897476634704283 1101 74-75 2.5153946583313633 1101 76-77 2.8116042072035583 1101 78-79 3.029413908552396 1101 80-81 3.234981341185339 1101 82-83 3.457933758845183 1101 84-85 3.861235284316148 1101 86-87 3.981667800285731 1101 88-89 4.228719436804184 1101 90-91 4.237734693685564 1101 92-93 4.706692221985374 1101 94-95 4.599708166071718 1101 96-97 4.735818473558066 1101 98-99 4.718289762879422 1101 100-101 3.902148737826817 1102 1 0.4216735319847089 1102 2 0.2951671951726098 1102 3 0.3365936145284252 1102 4 0.3120968716164967 1102 5 0.278773052596172 1102 6 0.2631864665302146 1102 7 0.3313270408465385 1102 8 0.3767212552129351 1102 9 0.46381497170006014 1102 10-11 0.44498787643953364 1102 12-13 0.5691779840577667 1102 14-15 0.5910300988432695 1102 16-17 0.5666686999506823 1102 18-19 0.5908684189470037 1102 20-21 0.5647774677456496 1102 22-23 0.6742345820366538 1102 24-25 0.6796302658658675 1102 26-27 0.7961473802969934 1102 28-29 0.9283284249526815 1102 30-31 0.7218004427713467 1102 32-33 0.8336851762332955 1102 34-35 0.8560557807517597 1102 36-37 0.9208440938457088 1102 38-39 1.0635665536660213 1102 40-41 1.1628821780867113 1102 42-43 1.09312476665405 1102 44-45 0.9904803080683493 1102 46-47 0.9990158306055861 1102 48-49 0.7826702221146391 1102 50-51 0.88863888924066 1102 52-53 0.8218203098891408 1102 54-55 1.0208440062932098 1102 56-57 1.3452169437581247 1102 58-59 1.4587795061562687 1102 60-61 1.5479709017483145 1102 62-63 1.5130547220283077 1102 64-65 1.4351156472619309 1102 66-67 1.7032572669812431 1102 68-69 1.8979690347034897 1102 70-71 1.975159942747144 1102 72-73 2.098170660618475 1102 74-75 2.4081221667576003 1102 76-77 2.5814533991489377 1102 78-79 2.6815021031768858 1102 80-81 2.963813118905506 1102 82-83 3.2899707478694715 1102 84-85 3.5245260543446655 1102 86-87 3.7882597110972434 1102 88-89 3.613893579056363 1102 90-91 3.915463498456109 1102 92-93 4.14275219951541 1102 94-95 4.081797008868364 1102 96-97 4.0788409003146135 1102 98-99 4.374678579749183 1102 100-101 4.14891327848882 1103 1 0.3521468682779343 1103 2 0.2484502103174009 1103 3 0.14506384293353847 1103 4 0.04970848426511054 1103 5 0.05215621080247956 1103 6 -0.03380800833535602 1103 7 -0.0172620766118996 1103 8 0.027971810192951807 1103 9 0.17330143018754995 1103 10-11 0.18200134395470258 1103 12-13 0.2538167537287208 1103 14-15 0.2854396001012205 1103 16-17 0.31896570059199547 1103 18-19 0.33203498695055345 1103 20-21 0.1628270212950511 1103 22-23 0.14990487457279045 1103 24-25 0.2616439800388193 1103 26-27 0.26264816960190984 1103 28-29 0.35787679454470833 1103 30-31 0.3579277921867643 1103 32-33 0.3134993195907967 1103 34-35 0.28753116430461745 1103 36-37 0.25018157102986294 1103 38-39 0.2097729569021709 1103 40-41 0.4602528060774418 1103 42-43 0.3432480955475441 1103 44-45 0.5652176175252066 1103 46-47 0.5636864191310664 1103 48-49 0.7158413779530264 1103 50-51 0.566395906654698 1103 52-53 0.9152111144868442 1103 54-55 0.8416933723827 1103 56-57 0.7298520135622795 1103 58-59 0.8870945868133617 1103 60-61 1.1448611636988915 1103 62-63 1.2130695214955196 1103 64-65 1.2360843957203187 1103 66-67 1.5583704829054774 1103 68-69 1.4857699572036118 1103 70-71 1.5319158995326951 1103 72-73 1.932302548537173 1103 74-75 2.0095108500983336 1103 76-77 2.2949542131196345 1103 78-79 2.4851347557347303 1103 80-81 2.7839718431914307 1103 82-83 3.15078175867308 1103 84-85 3.4844349142923754 1103 86-87 3.433127995838994 1103 88-89 3.7352615431429896 1103 90-91 3.8647259916730192 1103 92-93 3.9597691202395957 1103 94-95 4.108991029883608 1103 96-97 4.276188712460041 1103 98-99 4.3066318627643305 1103 100-101 4.073704236014343 1104 1 0.3168118368160293 1104 2 0.3342289651006567 1104 3 0.2060917922688219 1104 4 0.1430822935396847 1104 5 0.2263714078900705 1104 6 0.2707908960227883 1104 7 0.2929895022987381 1104 8 0.2712170967230705 1104 9 0.016707224760516226 1104 10-11 0.27278434405938157 1104 12-13 0.3264611818897407 1104 14-15 0.3952189596973952 1104 16-17 0.43046063528382916 1104 18-19 0.4852532895198465 1104 20-21 0.40083834990619494 1104 22-23 0.560968298511753 1104 24-25 0.5469557494655319 1104 26-27 0.6121050010801028 1104 28-29 0.6567299762095686 1104 30-31 0.7673861356972438 1104 32-33 0.7286190609169623 1104 34-35 0.9480857312236779 1104 36-37 1.0392676056670354 1104 38-39 0.8674633857377856 1104 40-41 1.175170749248288 1104 42-43 1.2905595858298256 1104 44-45 1.4279861486439955 1104 46-47 1.511029894711747 1104 48-49 1.7156145731098746 1104 50-51 2.1041269277417385 1104 52-53 2.0466474288499867 1104 54-55 1.9623506411871503 1104 56-57 2.2849714059579256 1104 58-59 2.210122838555108 1104 60-61 2.180312505336765 1104 62-63 2.413928472145116 1104 64-65 2.554058970315843 1104 66-67 2.5367774987352867 1104 68-69 2.663849559888046 1104 70-71 2.903904919852085 1104 72-73 3.2313883505539884 1104 74-75 3.4408994772893244 1104 76-77 3.6780915638064506 1104 78-79 3.851351883571244 1104 80-81 4.073668506252481 1104 82-83 4.430751983268031 1104 84-85 4.48478093706591 1104 86-87 4.904358673811011 1104 88-89 5.3248785337335 1104 90-91 5.779938781139972 1104 92-93 5.538182533129088 1104 94-95 5.596188187262907 1104 96-97 5.8149461708500745 1104 98-99 5.917489999242687 1104 100-101 4.950409633969613 1105 1 0.3787979350518498 1105 2 0.2021586535718498 1105 3 0.3536692837711328 1105 4 0.2214741783759635 1105 5 0.2603604043986465 1105 6 0.2626874532583443 1105 7 0.3276249272899534 1105 8 0.28979126018423784 1105 9 0.24816591549421219 1105 10-11 0.3039008183958245 1105 12-13 0.283327175844569 1105 14-15 0.36223157215641066 1105 16-17 0.6312494911409345 1105 18-19 0.5271086458191476 1105 20-21 0.3924116929004313 1105 22-23 0.33481422832098673 1105 24-25 0.47646219066362505 1105 26-27 0.4871178763726789 1105 28-29 0.4001671287986568 1105 30-31 0.4285510566518198 1105 32-33 0.5449721032154073 1105 34-35 0.5370460113900464 1105 36-37 0.5179878548616657 1105 38-39 0.3389201940149107 1105 40-41 0.21498292980783873 1105 42-43 0.27793291958937516 1105 44-45 0.366483983818938 1105 46-47 0.4683114498339833 1105 48-49 0.36598378322399583 1105 50-51 0.715591443435386 1105 52-53 0.8953334386458636 1105 54-55 0.8624072924744937 1105 56-57 1.0543100954129656 1105 58-59 1.0799154486699365 1105 60-61 1.1378187121157453 1105 62-63 1.3714178078510528 1105 64-65 1.1873084810547283 1105 66-67 1.0837803082129263 1105 68-69 1.1548412948381603 1105 70-71 1.4210104120079272 1105 72-73 1.7112762555929386 1105 74-75 1.6028955205787234 1105 76-77 1.9763348411278194 1105 78-79 2.4259442251696264 1105 80-81 2.4614074983213 1105 82-83 2.628486286954452 1105 84-85 2.9921589236898107 1105 86-87 3.035361667982496 1105 88-89 3.012550574280901 1105 90-91 3.412977847441514 1105 92-93 3.9325527448521775 1105 94-95 4.037488396464209 1105 96-97 3.9195530393302924 1105 98-99 3.821890507861145 1105 100-101 3.500479426934909 1106 1 0.3886275549732119 1106 2 0.20969305724158005 1106 3 0.18390683291923438 1106 4 0.08310261874293445 1106 5 0.177465581331802 1106 6 0.0797238228520527 1106 7 0.026312673030446376 1106 8 0.05560056555776072 1106 9 0.14582646595292914 1106 10-11 -6.658591926438362E-4 1106 12-13 -0.00248868260890589 1106 14-15 0.2540688919467158 1106 16-17 0.16416151604263263 1106 18-19 0.27392876377458464 1106 20-21 0.3205317780904764 1106 22-23 0.261246076289531 1106 24-25 0.21049233483138607 1106 26-27 0.28937295500963245 1106 28-29 0.28705687322853635 1106 30-31 0.2173895232311125 1106 32-33 0.2699639118654744 1106 34-35 0.21877848190118954 1106 36-37 0.5773096110870952 1106 38-39 0.6863251743556731 1106 40-41 0.7183634999257933 1106 42-43 0.2976937321712896 1106 44-45 0.36969007818328947 1106 46-47 0.35538795704236037 1106 48-49 0.48675786579279645 1106 50-51 0.6572739467119249 1106 52-53 0.7322944478201734 1106 54-55 0.6752046384771191 1106 56-57 0.6464382081259501 1106 58-59 1.064943299259717 1106 60-61 1.302151936231077 1106 62-63 1.0477529978903704 1106 64-65 1.1558340380665229 1106 66-67 1.3381782439927399 1106 68-69 1.0439173105655506 1106 70-71 1.61846454044829 1106 72-73 2.0138172123426124 1106 74-75 2.1400187184817376 1106 76-77 2.0973513876546868 1106 78-79 2.506035149153899 1106 80-81 2.604337544192859 1106 82-83 2.6174779317775183 1106 84-85 2.947457088827427 1106 86-87 2.955070055924832 1106 88-89 3.0401292112402736 1106 90-91 3.2115410846630077 1106 92-93 3.628111394917706 1106 94-95 3.515295572086753 1106 96-97 3.7506727968663363 1106 98-99 3.9154185222779176 1106 100-101 3.407914715270426 1107 1 0.33562627783400245 1107 2 0.2594072973437491 1107 3 0.22995249064592116 1107 4 0.125794189624159 1107 5 0.17947547916066497 1107 6 0.15415070663238595 1107 7 0.16242538056557265 1107 8 0.04402649148368454 1107 9 0.17901292827732362 1107 10-11 0.11229311271029019 1107 12-13 0.16280825225699402 1107 14-15 0.41398667611019135 1107 16-17 0.3282427740247513 1107 18-19 0.029166630952111916 1107 20-21 0.00930173339060758 1107 22-23 0.2340206612192901 1107 24-25 0.2703071496462002 1107 26-27 0.3420445705907369 1107 28-29 0.3981560111595712 1107 30-31 0.4113757939846252 1107 32-33 0.4234840268080049 1107 34-35 0.45239996976836494 1107 36-37 0.572185090014294 1107 38-39 0.6271042292726605 1107 40-41 0.6194594450088076 1107 42-43 0.2760749582249318 1107 44-45 0.4598289670721769 1107 46-47 0.6515541447818336 1107 48-49 0.8462877189217863 1107 50-51 0.9445935507987642 1107 52-53 1.0895406164025516 1107 54-55 1.009908501823226 1107 56-57 1.1331320140007861 1107 58-59 1.1923363388510282 1107 60-61 1.4222525109437214 1107 62-63 1.5896830745187245 1107 64-65 1.4490843573513317 1107 66-67 1.544253435563622 1107 68-69 1.9021995583305618 1107 70-71 2.137356622185198 1107 72-73 2.3021138279237086 1107 74-75 2.545095346834227 1107 76-77 2.406325685228122 1107 78-79 2.6686508898946393 1107 80-81 2.666798431804608 1107 82-83 2.89150331491928 1107 84-85 2.770774457920659 1107 86-87 3.026605815822659 1107 88-89 3.330049390039761 1107 90-91 3.4997546861955726 1107 92-93 3.432982084572881 1107 94-95 3.6561903677444825 1107 96-97 4.213499265576427 1107 98-99 4.086843330707037 1107 100-101 3.3544528698042697 1108 1 0.4765608027267447 1108 2 0.3429295784225772 1108 3 0.37382820647507486 1108 4 0.1833048010279157 1108 5 0.2759363772239709 1108 6 0.29325559435397963 1108 7 0.26667371282506025 1108 8 0.13093753603272518 1108 9 0.38434379586307443 1108 10-11 0.38243346686640933 1108 12-13 0.3448251813192087 1108 14-15 0.4904978072227095 1108 16-17 0.5923490962737006 1108 18-19 0.4906376854690748 1108 20-21 0.5151587036360468 1108 22-23 0.5633930596014736 1108 24-25 0.5158260960637335 1108 26-27 0.3782938471790871 1108 28-29 0.4420673032670521 1108 30-31 0.5156388813825927 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2316 92-93 4.05769472825104 2316 94-95 4.361337238753418 2316 96-97 4.655166844485382 2316 98-99 5.03217447465887 2316 100-101 5.2523492390799476 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4425.0 3 704.0 4 608.0 5 497.0 6 572.0 7 636.0 8 723.0 9 906.0 10 1240.0 11 1898.0 12 3023.0 13 4511.0 14 5346.0 15 7590.0 16 9850.0 17 13374.0 18 11871.0 19 7818.0 20 7872.0 21 8134.0 22 9188.0 23 10786.0 24 12497.0 25 14520.0 26 16653.0 27 18813.0 28 21509.0 29 24699.0 30 28395.0 31 32483.0 32 38315.0 33 45914.0 34 59495.0 35 86954.0 36 90886.0 37 125922.0 38 95836.0 39 7949.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.18033619181191 24.40990365749389 15.878725900806653 19.531034249887547 2 13.897523831038447 24.292458781585964 41.76310632013679 20.046911067238796 3 15.765290976548089 32.07598645743068 32.57115317357396 19.587569392447264 4 9.942614725482066 21.007937795564207 42.86854265438442 26.18090482456931 5 9.255519655358105 42.090857950611586 36.70510712362489 11.948515270405416 6 23.303363223309372 44.29081424141164 19.09921388561675 13.306608649662236 7 22.937661782060186 37.763447889652916 24.78014463947086 14.518745688816036 8 23.414666386276924 36.60972752125454 22.219605251299306 17.756000841169225 9 26.13084107657032 17.893126505184323 24.14351707197485 31.832515346270508 10-11 21.338272317056315 30.399204475127835 29.82491242617061 18.437610781645247 12-13 23.003808792488407 30.355517341452025 29.494137800297658 17.146536065761904 14-15 20.965225285523484 31.102157595624607 28.78444411466241 19.148173004189502 16-17 16.574647047831576 34.31169144857446 30.284338519238197 18.829322984355766 18-19 16.339149081134277 32.067022793349395 34.90854601898955 16.68528210652678 20-21 17.203274387704763 31.474189530155527 35.06951434001787 16.253021742121838 22-23 17.693448777853014 30.96394091108725 34.66135386356823 16.68125644749151 24-25 17.100255580187458 32.23519386655626 34.61720361742417 16.047346935832127 26-27 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33.82246753355977 18.215868637220495 52-53 16.538609170042054 31.982217117676136 34.036042713997986 17.443130998283824 54-55 16.98672761730846 31.488915653242106 33.64925949175094 17.875097237698494 56-57 17.04918584268556 32.01579175642441 32.976525020634774 17.95849738025526 58-59 16.53102157006321 31.023131848956098 33.94186913316686 18.50397744781383 60-61 17.220605328840072 32.02572212060323 32.988349357097164 17.765323193459544 62-63 16.89405197985009 31.627502970465937 33.861990170130504 17.616454879553466 64-65 17.015004811776056 31.024541860048803 33.65491936520599 18.305533962969147 66-67 16.900006547572925 31.637211598935327 32.705427096980365 18.757354756511383 68-69 16.713391437442972 30.98600179129976 33.526139340534975 18.774467430722293 70-71 17.271531740772623 30.963616778540022 33.67302861887222 18.091822861815128 72-73 17.336087191077116 30.48719972797845 33.26298500397098 18.91372807697345 74-75 16.827881930594334 31.46713299147928 32.63122293722156 19.073762140704822 76-77 16.917713956867722 30.847910095134658 33.45968295655716 18.774692991440464 78-79 16.783274753963646 30.375030110657043 33.25808422333728 19.58361091204204 80-81 17.221232367333748 30.91094549897697 32.29686916174175 19.570952971947523 82-83 17.5678468274069 30.410324924668036 32.53259579294402 19.48923245498104 84-85 16.971329405185323 30.087154907952428 32.70540631943664 20.236109367425605 86-87 17.037337369805744 30.432479186939126 33.039848151752146 19.490335291502987 88-89 17.782201036508507 29.631934940045685 33.15036823328531 19.4354957901605 90-91 17.391489806536264 29.50403064467485 33.397697083444974 19.706782465343906 92-93 17.94928634549661 29.77031733130041 32.96471646959242 19.315679853610565 94-95 17.556315379342124 28.666184817422995 33.52421052125634 20.25328928197854 96-97 18.14578751101688 28.494056140886315 32.963710828115026 20.396445519981786 98-99 17.675408057395078 28.786752800935755 32.843326025978456 20.694513115690707 100-101 18.25936360274331 27.777119645450416 33.760564777760074 20.2029519740462 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7575.0 1 4397.5 2 1725.5 3 2035.0 4 1453.0 5 1552.5 6 3291.0 7 6176.0 8 8355.0 9 8245.5 10 5975.5 11 3943.5 12 3458.5 13 3268.0 14 2907.5 15 2494.0 16 2251.5 17 2288.5 18 2432.0 19 2563.5 20 2792.5 21 3071.5 22 3395.0 23 3768.0 24 3981.0 25 4801.5 26 7233.5 27 9530.0 28 9794.5 29 11047.0 30 14903.0 31 18869.5 32 24009.5 33 30890.5 34 37299.0 35 42281.5 36 48611.0 37 54141.0 38 52308.0 39 47693.0 40 43381.0 41 38364.0 42 33224.5 43 31231.5 44 30721.5 45 28091.0 46 23520.0 47 19149.0 48 18889.5 49 16852.5 50 11731.5 51 7615.5 52 4981.0 53 3535.5 54 2719.5 55 2263.5 56 1915.0 57 1643.0 58 1425.0 59 1252.0 60 1109.0 61 994.5 62 857.0 63 662.0 64 506.0 65 427.5 66 366.5 67 320.5 68 289.0 69 256.0 70 200.0 71 164.0 72 147.5 73 128.5 74 108.5 75 81.5 76 61.0 77 49.5 78 44.0 79 39.0 80 27.0 81 12.5 82 8.0 83 9.5 84 5.5 85 2.0 86 0.5 87 2.0 88 3.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3823827623820897 2 0.023906430950058385 3 0.04432901015362585 4 0.0380821035737111 5 0.057183221769988905 6 0.05670269049461084 7 0.03291639236339697 8 0.028471478066149934 9 0.03459825182722017 10-11 0.030874134443040224 12-13 0.031054333671306996 14-15 0.02552822400445933 16-17 0.03033353675823991 18-19 0.026128888098681902 20-21 0.03814216998313335 22-23 0.025047692729081274 24-25 0.04607093602687131 26-27 0.038502568439666894 28-29 0.03099426726188474 30-31 0.03826230280197787 32-33 0.03531904874028726 34-35 0.033937521323575345 36-37 0.03477845105548694 38-39 0.01789979000783266 40-41 0.013875340576541426 42-43 0.013154543663474336 44-45 0.010631754467739533 46-47 0.0169987938664988 48-49 0.015196801583831082 50-51 0.010932086514850819 52-53 0.004625113525513808 54-55 0.005345910438580894 56-57 0.009490492688716646 58-59 0.003784183793602207 60-61 0.006547238627026041 62-63 0.00612677376107024 64-65 0.009490492688716646 66-67 0.005105644800891866 68-69 0.006667371445870554 70-71 0.017599457960721373 72-73 0.015016602355564311 74-75 0.010571688058317276 76-77 0.0078686996343157 78-79 0.0078686996343157 80-81 0.009490492688716646 82-83 0.01153275060907339 84-85 0.00522577761973638 86-87 2.40265637689029E-4 88-89 0.02342589967468033 90-91 0.019581649471655863 92-93 0.013394809301163366 94-95 0.024206762997169673 96-97 0.020002114337611662 98-99 0.017479325141876858 100-101 0.1122641192101988 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 832412.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.388179932079684 #Duplication Level Percentage of deduplicated Percentage of total 1 75.97219348005203 14.729625570260197 2 9.084030074148961 3.522456191720464 3 3.496497659895427 2.0337217728644426 4 1.9015792662976378 1.474726438803627 5 1.1959739125205746 1.1593878705011111 6 0.8906646104653604 1.0361019436102843 7 0.6692876008798184 0.9083387902517495 8 0.5418881093350034 0.8404979333473213 9 0.4132288205752414 0.7210579253790467 >10 4.0959540999714825 17.467841101120648 >50 0.8597296596622381 11.796188994070562 >100 0.814415388791954 29.914408369802363 >500 0.05400467898239329 7.122483914279345 >1k 0.008069664675530032 2.4924661946355284 >5k 0.0012414868731584665 1.8916747294679497 >10k+ 0.0012414868731584665 2.889022259885363 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13933 1.6738105649606205 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10072 1.2099777514019499 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7869 0.9453251514874845 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7849 0.9429224951105942 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2952 0.3546320812290068 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2483 0.2982897891909295 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2308 0.2772665458931395 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1766 0.2121545580794126 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1612 0.19365410397735738 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1481 0.17791670470872598 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1279 0.15364987530213403 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1226 0.14728283590337476 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1167 0.1401949995915484 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1159 0.1392339370407923 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1150 0.13815274167119168 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1084 0.13022397562745372 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1043 0.12529853005482863 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 993 0.1192918891126029 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 985 0.11833082656184679 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 978 0.11748989682993517 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 970 0.11652883427917907 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 953 0.11448657635882231 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 941 0.11304498253268815 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 924 0.1110027246123314 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 920 0.11052219333695333 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 895 0.10751887286584048 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 881 0.10583701340201726 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 879 0.10559674776432824 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 863 0.103674622662816 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 862 0.1035544898439715 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 852 0.10235316165552635 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 846 0.10163236474245926 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 841 0.10103170064823669 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 838 0.10067130219170316 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 833 0.10007063809748058 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.006640942225725E-5 0.0 16-17 0.0 0.0 0.0 1.201328188445145E-4 0.0 18-19 0.0 0.0 0.0 1.8019922826677175E-4 0.0 20-21 0.0 0.0 0.0 6.006640942225726E-4 0.0 22-23 0.0 0.0 0.0 0.002642922014579319 0.0 24-25 0.0 0.0 0.0 0.007688500406048928 0.0 26-27 0.0 0.0 0.0 0.014956535946142055 0.0 28-29 0.0 0.0 0.0 0.030213403939395395 0.0 30-31 0.0 0.0 0.0 0.072620288991509 0.0 32-33 0.0 0.0 0.0 0.14962542587084282 0.0 34-35 0.0 0.0 0.0 0.2557627713199714 0.0 36-37 0.0 0.0 0.0 0.4162001508868205 1.201328188445145E-4 38-39 0.0 0.0 0.0 0.6494380186734454 1.201328188445145E-4 40-41 0.0 0.0 0.0 0.9006357428773252 1.201328188445145E-4 42-43 0.0 0.0 0.0 1.1465476230520464 1.201328188445145E-4 44-45 0.0 0.0 0.0 1.4253158291807422 1.201328188445145E-4 46-47 0.0 0.0 0.0 1.7394631504591476 1.201328188445145E-4 48-49 0.0 0.0 0.0 2.0584157844913338 1.201328188445145E-4 50-51 0.0 0.0 0.0 2.3764073559727636 1.201328188445145E-4 52-53 0.0 0.0 0.0 2.6864701614104556 1.201328188445145E-4 54-55 0.0 0.0 0.0 3.0142525576277133 1.201328188445145E-4 56-57 0.0 0.0 0.0 3.3771738033569916 1.201328188445145E-4 58-59 0.0 0.0 0.0 3.7646021441305506 1.201328188445145E-4 60-61 0.0 0.0 0.0 4.177018111223768 1.201328188445145E-4 62-63 6.006640942225725E-5 0.0 0.0 4.555616689812257 1.201328188445145E-4 64-65 1.201328188445145E-4 0.0 0.0 4.933975002763054 1.201328188445145E-4 66-67 1.201328188445145E-4 0.0 0.0 5.367654478791752 1.201328188445145E-4 68-69 1.201328188445145E-4 0.0 0.0 5.818032416639838 1.201328188445145E-4 70-71 1.201328188445145E-4 0.0 0.0 6.292016453390869 1.201328188445145E-4 72-73 1.201328188445145E-4 0.0 0.0 6.747980567315224 1.201328188445145E-4 74-75 1.201328188445145E-4 0.0 0.0 7.213675439565984 1.201328188445145E-4 76-77 1.201328188445145E-4 0.0 0.0 7.71330783314032 1.201328188445145E-4 78-79 1.201328188445145E-4 0.0 0.0 8.256428307136371 1.201328188445145E-4 80-81 1.201328188445145E-4 0.0 0.0 8.819010297785232 1.201328188445145E-4 82-83 1.201328188445145E-4 0.0 0.0 9.35942778335728 1.201328188445145E-4 84-85 1.201328188445145E-4 0.0 0.0 9.886630658856433 1.201328188445145E-4 86-87 1.201328188445145E-4 0.0 0.0 10.43209372281995 1.201328188445145E-4 88-89 1.201328188445145E-4 0.0 0.0 10.990831463265787 1.201328188445145E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGGTCC 30 1.4849702E-6 47.535767 94-95 TTAACGC 20 5.377679E-4 47.520054 22-23 GTATGGT 90 1.1479642E-8 42.41855 1 GGTATCA 5800 0.0 33.240055 1 AGAGACG 30 0.0039369226 31.690512 94-95 TAACGCT 60 0.0039375387 31.687653 4 AGAACCG 60 0.0039375387 31.687653 5 CTAACGC 60 0.0039375387 31.687653 3 AACGCTA 60 0.0039375387 31.687653 5 GTATCAA 8590 0.0 30.443583 1 GTAATAC 125 2.1402411E-7 30.420149 3 TACCTGG 145 2.383058E-8 29.502298 2 TACAGGG 65 0.005824271 29.25014 2 GTACATG 5245 0.0 27.93195 1 GGATATG 325 0.0 27.898355 1 GTGGTAC 155 4.3823093E-8 27.708893 1 GTATAGT 700 0.0 27.26907 1 GACATAT 210 4.0017767E-11 27.26907 1 ACGCTAA 70 0.008375463 27.152681 6 GGGATGC 195 5.347829E-10 26.796516 7 >>END_MODULE