##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938215_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 832412 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49329178339572 33.0 31.0 34.0 31.0 34.0 2 32.70419575883096 34.0 31.0 34.0 31.0 34.0 3 32.741445341970085 34.0 31.0 34.0 31.0 34.0 4 36.219654449959876 37.0 35.0 37.0 35.0 37.0 5 36.16912298236931 37.0 37.0 37.0 35.0 37.0 6 36.24105611163703 37.0 37.0 37.0 35.0 37.0 7 36.213629789094824 37.0 37.0 37.0 35.0 37.0 8 36.22347587492732 37.0 37.0 37.0 35.0 37.0 9 37.890573417970906 39.0 38.0 39.0 35.0 39.0 10-11 37.88723973224798 39.0 38.0 39.0 35.0 39.0 12-13 37.830410301629485 39.0 38.0 39.0 35.0 39.0 14-15 39.010427528675706 40.0 38.0 41.0 35.5 41.0 16-17 38.95956809848969 40.0 38.0 41.0 35.5 41.0 18-19 39.24482648015646 40.5 39.0 41.0 36.0 41.0 20-21 39.26032841910016 41.0 39.0 41.0 36.0 41.0 22-23 39.13094897718918 40.5 39.0 41.0 35.0 41.0 24-25 39.016381911841734 40.0 38.5 41.0 35.0 41.0 26-27 38.8184727034209 40.0 38.0 41.0 35.0 41.0 28-29 38.687510511621646 40.0 38.0 41.0 35.0 41.0 30-31 38.386895551721985 40.0 37.5 41.0 34.5 41.0 32-33 38.07383302979774 40.0 36.5 41.0 34.0 41.0 34-35 37.88797314310702 40.0 36.0 41.0 34.0 41.0 36-37 37.77232788571044 40.0 36.0 41.0 33.5 41.0 38-39 37.56396231673739 40.0 36.0 41.0 33.0 41.0 40-41 37.37456151521122 40.0 35.5 41.0 33.0 41.0 42-43 37.098133496393615 40.0 35.0 41.0 33.0 41.0 44-45 36.76082516830608 40.0 35.0 41.0 31.5 41.0 46-47 36.30984776769196 40.0 35.0 41.0 30.5 41.0 48-49 35.86419585493722 39.0 35.0 41.0 29.5 41.0 50-51 35.54244652888233 39.0 35.0 41.0 27.5 41.0 52-53 35.147784390422046 39.0 35.0 40.0 26.5 41.0 54-55 34.8771515787855 38.5 35.0 40.0 25.5 41.0 56-57 34.58761646876787 38.0 35.0 40.0 24.0 41.0 58-59 34.2009798032705 38.0 34.0 40.0 22.5 41.0 60-61 33.842950365924565 38.0 34.0 40.0 21.5 41.0 62-63 33.38760733867363 37.5 33.0 40.0 19.0 41.0 64-65 32.91707651980029 37.0 33.0 39.5 15.0 41.0 66-67 32.437177143049354 36.0 32.0 39.0 8.0 40.0 68-69 31.90631382056001 36.0 31.5 39.0 6.0 40.0 70-71 31.404795341729816 35.0 31.0 38.0 2.0 40.0 72-73 30.791903528541155 35.0 30.5 37.5 2.0 39.5 74-75 30.172651283258773 35.0 29.5 37.0 2.0 39.0 76-77 28.73937365150911 33.0 28.0 35.5 2.0 38.0 78-79 29.423404516032925 34.0 29.0 36.0 2.0 38.0 80-81 29.144568434861583 34.0 29.0 36.0 2.0 37.5 82-83 28.62920765198003 34.0 29.0 35.0 2.0 37.0 84-85 28.028188565277773 34.0 27.5 35.0 2.0 36.5 86-87 27.44503142674541 33.5 26.0 35.0 2.0 36.0 88-89 26.73622857431176 33.0 25.5 35.0 2.0 35.5 90-91 26.18159457095765 32.5 24.5 35.0 2.0 35.0 92-93 25.394846542337206 32.0 20.5 34.5 2.0 35.0 94-95 24.622875451098736 31.5 15.5 34.0 2.0 35.0 96-97 23.704892529180263 31.0 2.0 34.0 2.0 35.0 98-99 22.444292609909517 31.0 2.0 34.0 2.0 35.0 100-101 18.428543798023092 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2503735449911346 1101 2 0.19170792979273443 1101 3 0.26438888673309435 1101 4 0.11526056452161981 1101 5 0.06803709887393694 1101 6 0.16943557636609796 1101 7 0.21392790246812154 1101 8 0.1989703965244516 1101 9 0.23898050801398085 1101 10-11 0.20862626119791372 1101 12-13 0.33430117856858743 1101 14-15 0.3990693799486493 1101 16-17 0.3968354696895986 1101 18-19 0.3953303576047702 1101 20-21 0.33427863273062286 1101 22-23 0.29151562150218524 1101 24-25 0.41493640120281583 1101 26-27 0.3799103768654035 1101 28-29 0.3222496696531181 1101 30-31 0.2735323932771365 1101 32-33 0.2673282629055649 1101 34-35 0.2575040829471149 1101 36-37 0.3532082432460655 1101 38-39 0.39049605131636156 1101 40-41 0.41533185556206575 1101 42-43 0.5655301182573353 1101 44-45 0.510278719122006 1101 46-47 0.455955196237781 1101 48-49 0.03169280664557306 1101 50-51 -0.021873132865927403 1101 52-53 -0.0743383582773447 1101 54-55 0.014027835202533367 1101 56-57 0.1026364181070889 1101 58-59 0.42644979061785193 1101 60-61 0.2556177479858448 1101 62-63 0.31388686739271776 1101 64-65 0.55004396379924 1101 66-67 0.8714859919648035 1101 68-69 0.8056498024112244 1101 70-71 1.065409907445769 1101 72-73 1.1672684176191161 1101 74-75 1.1551716293086045 1101 76-77 1.242584890062158 1101 78-79 1.4342419817824172 1101 80-81 1.2687534540142558 1101 82-83 1.1753534116155286 1101 84-85 1.331202357987717 1101 86-87 1.5069232745483774 1101 88-89 1.4649122736667444 1101 90-91 1.7468801619256276 1101 92-93 1.863756646506932 1101 94-95 2.1636607099608725 1101 96-97 2.692268120713134 1101 98-99 3.076296690901568 1101 100-101 3.573560183085494 1102 1 0.1572398486813924 1102 2 0.04125420928456691 1102 3 0.07489791793177858 1102 4 0.05100509826978339 1102 5 -0.15579187010022366 1102 6 -0.1010228974045404 1102 7 -0.06692293151338191 1102 8 0.015686756389634127 1102 9 0.17016623094199446 1102 10-11 0.13457043196967078 1102 12-13 0.16823299085793053 1102 14-15 0.27459331910689144 1102 16-17 0.2526160850210246 1102 18-19 0.16769985894433148 1102 20-21 0.07855203174539582 1102 22-23 0.14000538038214927 1102 24-25 0.21614632255793254 1102 26-27 0.20292913064641027 1102 28-29 0.19579128230543574 1102 30-31 0.2661464715763344 1102 32-33 0.36176694581973834 1102 34-35 0.4319045237041763 1102 36-37 0.41422176843835956 1102 38-39 0.3955247869485419 1102 40-41 0.5505394867089066 1102 42-43 0.6644293058815691 1102 44-45 0.5798967296655988 1102 46-47 0.410697655835051 1102 48-49 0.523644669060225 1102 50-51 0.6174179828544055 1102 52-53 0.6108076965148115 1102 54-55 0.40346586994455436 1102 56-57 0.6190146481649919 1102 58-59 0.681690262487173 1102 60-61 0.5173604668519758 1102 62-63 0.9859700926973574 1102 64-65 0.8084421143480327 1102 66-67 0.8348729075295722 1102 68-69 1.1988450750756385 1102 70-71 1.0475257574155137 1102 72-73 1.2571763771731703 1102 74-75 1.312036852711941 1102 76-77 0.9445099613610921 1102 78-79 1.2053950800453173 1102 80-81 1.2821987050729362 1102 82-83 1.45224175315904 1102 84-85 1.6004681949940256 1102 86-87 1.4252211744706003 1102 88-89 1.7174217801916818 1102 90-91 1.953122059773694 1102 92-93 2.0901783040991866 1102 94-95 2.357435016416666 1102 96-97 2.773513259421975 1102 98-99 3.2990000009007083 1102 100-101 3.2725414725159645 1103 1 0.26449898735240396 1103 2 0.23677983702863514 1103 3 0.16142546960658422 1103 4 0.09414554521369922 1103 5 -0.014278131261484361 1103 6 0.047088937236082984 1103 7 0.06260323887781283 1103 8 0.11330774203415217 1103 9 0.2913862003564276 1103 10-11 0.33396858696942644 1103 12-13 0.33763139251546903 1103 14-15 0.4270309146867888 1103 16-17 0.3237676068995725 1103 18-19 0.3653498268788198 1103 20-21 0.44605055179867037 1103 22-23 0.41619427091542605 1103 24-25 0.4567856595418007 1103 26-27 0.3986890832881116 1103 28-29 0.32774086545377656 1103 30-31 0.3340667764453684 1103 32-33 0.3404187143560762 1103 34-35 0.5419663114798183 1103 36-37 0.46762626254324147 1103 38-39 0.5457177966668638 1103 40-41 0.7123254124155807 1103 42-43 0.6215940979490568 1103 44-45 0.551025435698854 1103 46-47 0.5303945134148478 1103 48-49 0.4137154598450934 1103 50-51 0.5098166065039536 1103 52-53 0.6980690551059041 1103 54-55 0.6179625647302132 1103 56-57 0.6327750694564926 1103 58-59 0.6231367870789555 1103 60-61 0.7278767216001398 1103 62-63 0.5948263405391074 1103 64-65 0.43371343791371686 1103 66-67 0.45340430706585977 1103 68-69 0.6471548525903188 1103 70-71 0.6788802786527555 1103 72-73 0.9023776499273559 1103 74-75 1.1241853653654843 1103 76-77 0.8974538220487709 1103 78-79 0.8531153454490976 1103 80-81 1.2240429653462357 1103 82-83 1.1995716826149128 1103 84-85 1.345504207030924 1103 86-87 1.4689474336585988 1103 88-89 1.6413802676861273 1103 90-91 1.9671260556298442 1103 92-93 1.9459266638249026 1103 94-95 1.9235238872173142 1103 96-97 2.219522953073003 1103 98-99 2.7001395598765825 1103 100-101 2.8762826545000806 1104 1 0.093063603470668 1104 2 0.15834606985834654 1104 3 0.21413724135949508 1104 4 0.1338945478539344 1104 5 0.025581366045933862 1104 6 -0.05573507383664378 1104 7 0.07879943088265406 1104 8 0.13400513784930723 1104 9 0.0363703240174047 1104 10-11 0.14244352794201376 1104 12-13 0.249849194951004 1104 14-15 0.42873195101045525 1104 16-17 0.3397282707980551 1104 18-19 0.2425001112572005 1104 20-21 0.22196736676081485 1104 22-23 0.27927974726374316 1104 24-25 0.16180560673698352 1104 26-27 0.15849403112300564 1104 28-29 0.24695472497434423 1104 30-31 0.24062899657400294 1104 32-33 0.33952603375544754 1104 34-35 0.476030072718288 1104 36-37 0.3622114423006195 1104 38-39 0.43046895104535565 1104 40-41 0.4634623443857535 1104 42-43 0.54696948904202 1104 44-45 0.4416386229466056 1104 46-47 0.7126670979432674 1104 48-49 0.8945562661072373 1104 50-51 0.8525303555298578 1104 52-53 0.7929752509489774 1104 54-55 0.9245561886063669 1104 56-57 0.8502605597620416 1104 58-59 0.7846654517218283 1104 60-61 1.0744228909244953 1104 62-63 1.2431072761381543 1104 64-65 1.113191670464282 1104 66-67 1.075995232545928 1104 68-69 1.22345142027703 1104 70-71 1.1870045444447683 1104 72-73 1.3790458286263068 1104 74-75 1.315675531340105 1104 76-77 1.357123268528646 1104 78-79 1.2509106860634454 1104 80-81 1.5957324869624792 1104 82-83 1.6410909261335291 1104 84-85 1.7046991079928127 1104 86-87 1.9975859067386104 1104 88-89 2.324952618431098 1104 90-91 2.395592389649412 1104 92-93 2.8033943586735255 1104 94-95 3.0176595281445415 1104 96-97 3.0176201185940137 1104 98-99 3.524679843906032 1104 100-101 3.3331257637037055 1105 1 0.22507175841460025 1105 2 0.22345242865456783 1105 3 0.252855466025359 1105 4 0.17961870758570342 1105 5 0.026607837647475208 1105 6 0.0962342243622345 1105 7 0.0965105057653588 1105 8 0.03267392139166958 1105 9 0.15841211455326487 1105 10-11 0.018324819520323388 1105 12-13 0.2586372481002144 1105 14-15 0.22068764579676525 1105 16-17 0.32458603877285697 1105 18-19 0.25749494338113976 1105 20-21 0.27320392761921397 1105 22-23 0.267027796434931 1105 24-25 0.2800878118395005 1105 26-27 0.22369184889409865 1105 28-29 0.2111232297000214 1105 30-31 0.3360073881036527 1105 32-33 0.5645621733697936 1105 34-35 0.44327355249254197 1105 36-37 0.37428846674058747 1105 38-39 0.254191988406788 1105 40-41 0.2962451479673973 1105 42-43 0.4048830878372556 1105 44-45 0.5676078216949492 1105 46-47 0.47088242256047863 1105 48-49 0.4942367812610087 1105 50-51 0.47989274973046747 1105 52-53 0.4804936759366285 1105 54-55 0.6238781490605732 1105 56-57 0.5113002795956731 1105 58-59 0.8010721262735032 1105 60-61 0.8959571285473373 1105 62-63 1.0384229783122194 1105 64-65 0.955376101482905 1105 66-67 1.0051136390642057 1105 68-69 1.028330357008727 1105 70-71 1.194195173362857 1105 72-73 1.2542105435762103 1105 74-75 1.213238815623015 1105 76-77 1.2938851590833345 1105 78-79 1.273408705932244 1105 80-81 1.2843359883317014 1105 82-83 1.2408842110912808 1105 84-85 1.4378520096397835 1105 86-87 1.5083718501157364 1105 88-89 1.6965602008240914 1105 90-91 1.8313873013337805 1105 92-93 2.111263027137408 1105 94-95 2.3577146521558063 1105 96-97 2.607271224803636 1105 98-99 2.8715508814237474 1105 100-101 3.340042903648367 1106 1 0.2369524923595563 1106 2 0.11039214031904976 1106 3 0.2058174581616612 1106 4 0.1768959028674857 1106 5 -0.03434072067493332 1106 6 0.09861790719316588 1106 7 0.09059143630270938 1106 8 0.1651264115620492 1106 9 0.18608577117187508 1106 10-11 0.159965546912197 1106 12-13 0.18118557706483074 1106 14-15 0.28609475588850586 1106 16-17 0.1594623493888392 1106 18-19 0.06076738767996659 1106 20-21 0.1019572041591843 1106 22-23 0.13285020796836022 1106 24-25 0.10052850646597733 1106 26-27 -0.02416857050301502 1106 28-29 0.11587748920199203 1106 30-31 0.2809525060590943 1106 32-33 0.07575401478526089 1106 34-35 0.18817607542831638 1106 36-37 0.25580222820847354 1106 38-39 0.2686210513163587 1106 40-41 0.11374350969740732 1106 42-43 0.14361752427237917 1106 44-45 0.11621856874607062 1106 46-47 0.16652380526033994 1106 48-49 0.19243058860045892 1106 50-51 0.2983806265325697 1106 52-53 0.2697875815722881 1106 54-55 0.4184874216687007 1106 56-57 0.2745678090845374 1106 58-59 0.23664715903890254 1106 60-61 0.23952282317381446 1106 62-63 0.13739250649046397 1106 64-65 0.1660355051526281 1106 66-67 0.6064389994836006 1106 68-69 0.6046065693285172 1106 70-71 0.669587539024711 1106 72-73 0.8809479288973137 1106 74-75 0.9886819676544718 1106 76-77 0.9639711682576433 1106 78-79 1.1106752524591101 1106 80-81 0.7612863487510992 1106 82-83 1.3692819830440968 1106 84-85 1.3667756662583948 1106 86-87 1.2942728986085292 1106 88-89 1.5651660330652426 1106 90-91 1.5048030942564559 1106 92-93 1.3689211201911426 1106 94-95 1.6911419129683871 1106 96-97 2.2093968801116297 1106 98-99 2.427433908946682 1106 100-101 2.7629868748148176 1107 1 0.18948761368778122 1107 2 0.08403837275838555 1107 3 0.09916356288707817 1107 4 0.05419794629022334 1107 5 -0.04771396460851918 1107 6 0.005458584076940554 1107 7 0.016889329023015875 1107 8 0.2045437167983195 1107 9 0.1168456690007389 1107 10-11 0.11285906543071889 1107 12-13 0.20511038549394556 1107 14-15 0.2472146473316741 1107 16-17 0.2038453315088944 1107 18-19 0.09689845409120323 1107 20-21 0.09970464988077055 1107 22-23 0.03416246850475346 1107 24-25 0.2685090300930497 1107 26-27 0.31180524814321586 1107 28-29 0.27657033722242375 1107 30-31 0.4044766120616501 1107 32-33 0.46681164569203304 1107 34-35 0.5098898366032856 1107 36-37 0.5752211298687584 1107 38-39 0.5514155915462453 1107 40-41 0.6936588992248645 1107 42-43 0.6531745166095391 1107 44-45 0.793498262232653 1107 46-47 0.8639224323356913 1107 48-49 1.0097557801713393 1107 50-51 0.9173062331736901 1107 52-53 0.9018357935773906 1107 54-55 0.7426261509151857 1107 56-57 0.9924980453552905 1107 58-59 0.9078237235344275 1107 60-61 0.6864217695339647 1107 62-63 0.8667172191341379 1107 64-65 1.0503003518959204 1107 66-67 1.1851283353712034 1107 68-69 1.204193095509865 1107 70-71 1.1007664981562613 1107 72-73 1.0788558152319219 1107 74-75 1.1920655883441285 1107 76-77 1.1483733074658176 1107 78-79 0.9613816700836324 1107 80-81 0.9598806974100995 1107 82-83 1.3470374108857328 1107 84-85 1.3592485270502195 1107 86-87 1.5508597440746854 1107 88-89 1.6996216524777594 1107 90-91 1.460625252621714 1107 92-93 1.789890947777348 1107 94-95 1.7714675834664746 1107 96-97 2.18163905763398 1107 98-99 2.503980843812581 1107 100-101 2.45764683650064 1108 1 0.3112207850424795 1108 2 0.19863989384921865 1108 3 0.1305543002669225 1108 4 0.12626368207159544 1108 5 0.09547153863186963 1108 6 0.15956944762962166 1108 7 0.13620921550681686 1108 8 0.23120953094587549 1108 9 0.3468110600037164 1108 10-11 0.32283862136663544 1108 12-13 0.30365749619191007 1108 14-15 0.3597332347074982 1108 16-17 0.3660084020204195 1108 18-19 0.33439142490717444 1108 20-21 0.29749154305520875 1108 22-23 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80-81 0.74619706303681 2316 82-83 0.9139248401869544 2316 84-85 1.1486209043536313 2316 86-87 1.1896300414656729 2316 88-89 1.5883207949700058 2316 90-91 1.7651007133040757 2316 92-93 2.014784215429234 2316 94-95 2.283284770111244 2316 96-97 2.584248070587819 2316 98-99 3.15975533751811 2316 100-101 3.924236874814813 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 3.0 5 30.0 6 54.0 7 148.0 8 239.0 9 452.0 10 853.0 11 1572.0 12 2460.0 13 3899.0 14 4596.0 15 6421.0 16 8628.0 17 12304.0 18 11726.0 19 7280.0 20 5941.0 21 6156.0 22 6687.0 23 7361.0 24 8558.0 25 9704.0 26 10561.0 27 11755.0 28 14307.0 29 17225.0 30 21015.0 31 25518.0 32 31698.0 33 40930.0 34 57653.0 35 90720.0 36 110211.0 37 171799.0 38 118387.0 39 5559.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.304094606997495 24.770185917550442 16.158464798681422 19.767254676770637 2 13.79610717289439 24.467980295566502 41.606271777003485 20.129640754535625 3 15.702203320598764 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17.58777670392192 31.113194751868217 34.48196532840468 16.817063215805184 24-25 17.107966951282876 32.34936013911456 34.47548226079174 16.06719064881083 26-27 16.337175181425053 32.14213416293384 35.233913825427074 16.286776830214023 28-29 16.196606968664558 31.1342219958386 35.94674271874985 16.722428316746996 30-31 16.64109839838926 31.61883778705737 35.28661287919924 16.453450935354127 32-33 15.99075938357448 32.3742930183611 35.15278491900645 16.482162679057964 34-35 16.554351574073294 31.644922512798395 35.122985927473565 16.677739985654746 36-37 17.136989477503192 31.04094511458938 34.48211031382485 17.339955094082583 38-39 16.117082113903187 32.333640894040535 34.6856393177919 16.86363767426438 40-41 15.939952603645736 31.608682723758548 35.95142624580626 16.499938426789456 42-43 16.18529019546596 31.459807537513367 34.83006355346781 17.524838713552867 44-45 16.018361618402945 31.649704434888275 34.71443923120033 17.617494715508446 46-47 16.888331739571267 31.39340855249564 34.09321345679784 17.625046251135252 48-49 16.502885590308644 31.58550092982802 33.925868440147426 17.98574503971591 50-51 16.307309361229773 31.37454769993705 33.97097831362354 18.34716462520963 52-53 16.372421349043503 31.918749369302702 34.15027654574898 17.55855273590482 54-55 16.904129205249323 31.494680518781564 33.52360369624657 18.077586579722542 56-57 16.895900107158475 32.04993440748091 32.83674430450306 18.217421180857556 58-59 16.31782098287867 31.154103977357366 33.86586209713459 18.66221294262937 60-61 17.10306915325584 32.41297578602904 32.53088614772493 17.953068912990204 62-63 16.648126168291665 32.12838113818638 33.32184062699721 17.90165206652475 64-65 16.698762151434625 31.523452328894823 33.13076937862501 18.64701614104554 66-67 16.573403554970376 32.21229391214927 32.17457220703209 19.039730325848257 68-69 16.45975641694077 31.471421397731397 32.934409410792334 19.1344127745355 70-71 17.10787446600962 31.337426658914097 32.93315089162578 18.621547983450505 72-73 17.16536833044714 30.906408669269947 32.389569341605686 19.53865365867723 74-75 16.700083612441915 31.835196993796337 31.699086510045504 19.765632883716236 76-77 16.536222447537998 31.198432987510994 32.960961639188284 19.304382925762724 78-79 16.516520665247498 30.638253653239023 32.65444275190651 20.190782929606975 80-81 16.850400133107403 31.318442042718107 31.690794385702723 20.140363438471766 82-83 17.19118759194108 30.880429892316684 31.835866286958886 20.092516228783346 84-85 16.5242824121907 30.56938484235414 31.959508488992906 20.946824256462254 86-87 16.59259196965811 30.83319467479116 32.360320790792194 20.213892564758545 88-89 17.159711777341027 30.22595781896465 32.18670562173539 20.427624781958933 90-91 16.873020754744182 29.821069020697077 32.732691965765966 20.573218258792775 92-93 17.35877185816639 30.181208343945066 32.03455740666881 20.42546239121973 94-95 16.883716767488195 29.052277629513767 32.53823379422317 21.525771808774866 96-97 17.276663274904525 29.09783531893576 32.02386086647309 21.60164053968662 98-99 16.631667972109966 29.330187455250524 32.031193687741165 22.006950884898345 100-101 16.75318231837119 28.800882255421595 32.766646804707285 21.679288621499932 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7043.0 1 4119.0 2 1659.5 3 2004.0 4 1473.0 5 1522.5 6 3277.5 7 6314.0 8 8521.5 9 8272.5 10 5926.0 11 3832.5 12 3267.5 13 3060.5 14 2741.0 15 2385.5 16 2194.5 17 2167.5 18 2238.5 19 2353.5 20 2537.0 21 2779.5 22 3034.5 23 3357.5 24 3581.5 25 4513.5 26 7011.5 27 9224.0 28 9387.0 29 10429.0 30 14389.0 31 18305.5 32 23525.0 33 30614.5 34 36953.0 35 41915.0 36 48924.5 37 54418.5 38 52473.5 39 48370.5 40 43698.5 41 38267.5 42 32833.0 43 31630.5 44 31604.5 45 28987.5 46 24710.0 47 20462.5 48 20680.0 49 18030.5 50 12420.5 51 8522.0 52 5526.5 53 3716.0 54 2641.0 55 2149.5 56 1807.5 57 1491.0 58 1244.0 59 1077.0 60 941.0 61 800.5 62 624.0 63 460.5 64 306.5 65 205.5 66 156.5 67 152.5 68 130.0 69 96.0 70 86.5 71 73.5 72 61.0 73 57.0 74 50.5 75 35.5 76 26.5 77 19.0 78 13.0 79 7.0 80 5.0 81 5.5 82 3.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013454875710585623 3 0.003844250203024464 4 0.001922125101512232 5 0.11965228756913644 6 1.201328188445145E-4 7 0.0 8 3.6039845653354345E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.8019922826677172E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.8019922826677172E-4 24-25 5.405976848003152E-4 26-27 0.00522577761973638 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009730758326405674 36-37 6.006640942225724E-4 38-39 0.006727437855292812 40-41 0.008349230909693757 42-43 0.005045578391469609 44-45 0.002823121242846091 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 7.207969130670869E-4 70-71 0.0 72-73 6.006640942225725E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.8019922826677172E-4 82-83 0.0021023243297790036 84-85 0.00480531275378058 86-87 0.003784183793602207 88-89 0.0 90-91 0.0028831876522683476 92-93 0.0 94-95 6.006640942225725E-5 96-97 3.6039845653354345E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 832412.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.73198869616359 #Duplication Level Percentage of deduplicated Percentage of total 1 73.6639679620783 13.062086472181294 2 9.934546604069027 3.523185361697248 3 3.805233535496693 2.0242307411306992 4 2.0806292086637113 1.4757477443573093 5 1.3500012928438763 1.1969103832256924 6 0.9461184595681802 1.0065937098176807 7 0.704006158844234 0.8738400475458855 8 0.568371882625455 0.806269103834543 9 0.45231210279812767 0.7218353784558946 >10 4.566362609619044 17.719399437229722 >50 0.9530564612909292 11.938213505199517 >100 0.9022899594465971 30.718719068740835 >500 0.05956602883068308 7.213290790994536 >1k 0.010830187060124197 3.0420455771865376 >5k 0.0013537733825155246 1.8561939179794755 >10k+ 0.0013537733825155246 2.821438760423119 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13390 1.608578444328049 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10117 1.215383728249953 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7974 0.9579390974661586 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7491 0.899914945964258 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3152 0.3786586449979097 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2245 0.269698178305935 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2207 0.2651331311898435 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 2016 0.24218776279054124 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1874 0.22512890251462017 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1687 0.20266406539069595 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1469 0.1764751108825918 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1362 0.16362089926622872 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1340 0.16097797725164945 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1274 0.15304921120791148 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1266 0.15208814865715534 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1210 0.14536071080186255 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 1097 0.1317857022724324 RNA PCR Primer, Index 27 (95% over 23bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1086 0.13046424126514275 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1058 0.12710052233749636 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1002 0.12037308448220353 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 998 0.11989255320682547 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 992 0.11917175629375838 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 991 0.11905162347491387 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 971 0.11664896709802357 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 969 0.11640870146033455 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 965 0.11592817018495649 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 950 0.11412617790228877 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 927 0.11136312306886494 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 923 0.11088259179348689 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 922 0.11076245897464236 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 911 0.1094409979673527 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 869 0.1043954195758831 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 868 0.10427528675703858 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 860 0.10331422420628246 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 838 0.10067130219170316 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.006640942225725E-5 0.0 16-17 0.0 0.0 0.0 1.201328188445145E-4 0.0 18-19 0.0 0.0 0.0 1.8019922826677175E-4 0.0 20-21 0.0 0.0 0.0 6.006640942225726E-4 0.0 22-23 0.0 0.0 0.0 0.002642922014579319 0.0 24-25 0.0 0.0 0.0 0.007448234768359898 0.0 26-27 0.0 0.0 0.0 0.014776336717875283 0.0 28-29 0.0 0.0 0.0 0.029973138301706368 0.0 30-31 0.0 0.0 0.0 0.07328101949515384 0.0 32-33 0.0 0.0 0.0 0.15106701969697697 0.0 34-35 0.0 0.0 0.0 0.2579852284685949 0.0 36-37 0.0 0.0 0.0 0.4202246003181117 0.0 38-39 0.0 0.0 0.0 0.6547839291120263 0.0 40-41 0.0 0.0 0.0 0.9070027822760844 0.0 42-43 0.0 0.0 0.0 1.1547166547334733 0.0 44-45 0.0 0.0 0.0 1.4340254585469694 0.0 46-47 0.0 0.0 0.0 1.7517767643907103 0.0 48-49 0.0 0.0 0.0 2.073672652484587 0.0 50-51 0.0 0.0 0.0 2.394006813933485 0.0 52-53 0.0 0.0 0.0 2.7058716116538446 0.0 54-55 0.0 0.0 0.0 3.0355761329726145 0.0 56-57 0.0 0.0 0.0 3.4010201678976273 0.0 58-59 0.0 0.0 0.0 3.791271629914033 0.0 60-61 0.0 0.0 0.0 4.2044083939203185 0.0 62-63 0.0 0.0 0.0 4.582226109186316 0.0 64-65 0.0 0.0 0.0 4.964068273883606 0.0 66-67 0.0 0.0 0.0 5.401171535249372 0.0 68-69 0.0 0.0 0.0 5.85377193024608 0.0 70-71 0.0 0.0 0.0 6.3306992210588025 0.0 72-73 0.0 0.0 0.0 6.789726721863692 0.0 74-75 0.0 0.0 0.0 7.262749696063969 0.0 76-77 0.0 0.0 0.0 7.77133198464222 0.0 78-79 0.0 0.0 0.0 8.325684877200231 0.0 80-81 0.0 0.0 0.0 8.899799618458168 0.0 82-83 0.0 0.0 0.0 9.458837690951116 0.0 84-85 0.0 0.0 0.0 10.006162813606725 0.0 86-87 0.0 0.0 0.0 10.572288722411498 0.0 88-89 0.0 0.0 0.0 11.153671499209526 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCCTA 90 1.1972588E-8 42.216618 7 GCCTATG 75 6.9262915E-6 37.994957 9 GGTATCA 5400 0.0 34.614754 1 TCGTATC 35 2.0553292E-4 33.924065 94-95 GTATCAA 8585 0.0 33.57336 1 GTGGTAC 190 0.0 32.5426 1 GGTACTC 190 0.0 32.53868 3 CCTTTAG 195 0.0 31.708176 1 CTTTAGG 210 0.0 31.708174 2 ATGGGTA 300 0.0 31.70436 5 CATTCCG 180 5.456968E-12 31.662462 9 GGCATTC 200 0.0 30.870901 7 TGGTACT 205 1.8189894E-12 30.16143 2 GACTGGT 80 4.4960837E-4 29.683558 7 TAGGCAT 225 0.0 29.590736 5 GAGTACC 345 0.0 28.950941 1 AGTACCA 255 0.0 27.9778 2 GCGCACC 35 0.008398071 27.139252 92-93 ATCAACG 10680 0.0 26.98433 3 TCAACGC 10700 0.0 26.889444 4 >>END_MODULE