FastQCFastQC Report
Thu 26 May 2016
SRR938214_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938214_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827248
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141001.7044465504903004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102741.24194920991045No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80090.9681498172253061No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77470.9364785408970465No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30600.3699011662766184No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25240.3051080208111715No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23170.28008529485716493No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17760.21468773572133146No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17120.2069512407403826No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14960.18084057017968008No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12440.15037812119219388No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA12430.15025723845811656No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT12190.1473560528402607No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT11110.13430071755990947No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10960.1324874765487496No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA10730.1297071736649711No Hit
GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10350.12511362977003268No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT10350.12511362977003268No Hit
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10070.12172891321586755No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT9750.11786066572539312No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT9560.1155638937779239No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG9370.11326712183045472No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC9360.11314623909637739No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG8970.10843181246736167No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC8960.10831092973328434No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG8870.1072229851265884No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT8870.1072229851265884No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8810.10649768872212444No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC8800.10637680598804711No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA8710.10528886138135118No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA8540.10323385490203663No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA8370.10117884842272208No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG8370.10117884842272208No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT8360.10105796568864475No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG8310.10045355201825812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGT1251.2732926E-1038.190131
GGTCGAT250.001616590137.99727634-35
GGTATCA53600.037.8516921
ATTGCCG801.076208E-535.6299938
GCCGTGT401.0795402E-535.61706592-93
GTATCAA86000.034.8040851
CCTTTAG2150.033.3053471
TACCTGG2000.033.2747842
GTGGTAC1308.230927E-932.9051486
CATTCCG2250.031.6711049
GAGTGGG1051.898743E-631.67117
GGTAATC1051.898743E-631.67118
ATGTATT1252.0605148E-730.5521031
TAGGCAT2650.030.4944155
GGAGTGG804.4761685E-429.7060366
CTTGTCA650.005839136429.2348639
TGGTACT1503.32966E-828.5039947
TCAACGC104100.028.402524
ATCAACG104150.028.3888853
CATGGGT5400.028.1691324
Produced by FastQC (version 0.11.5)