##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938214_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 827248 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05608958861188 33.0 31.0 34.0 31.0 34.0 2 32.23065876254763 34.0 31.0 34.0 31.0 34.0 3 32.28315088099337 34.0 31.0 34.0 30.0 34.0 4 35.803566765951686 37.0 35.0 37.0 35.0 37.0 5 35.83707062452856 37.0 35.0 37.0 35.0 37.0 6 35.90082055199892 37.0 37.0 37.0 35.0 37.0 7 35.86000933214707 37.0 37.0 37.0 35.0 37.0 8 35.852396137554884 37.0 37.0 37.0 35.0 37.0 9 37.51532309537164 39.0 38.0 39.0 35.0 39.0 10-11 37.46801442856314 39.0 38.0 39.0 35.0 39.0 12-13 37.413076852407016 39.0 38.0 39.0 35.0 39.0 14-15 38.61698003500764 40.0 38.0 41.0 34.5 41.0 16-17 38.52046182039727 40.0 38.0 41.0 34.5 41.0 18-19 38.72745053478522 40.0 38.5 41.0 35.0 41.0 20-21 38.75640376283775 40.0 39.0 41.0 35.0 41.0 22-23 38.66033583641182 40.0 38.5 41.0 35.0 41.0 24-25 38.56765383536738 40.0 38.0 41.0 35.0 41.0 26-27 38.37785887666093 40.0 38.0 41.0 34.5 41.0 28-29 38.1827178790399 40.0 38.0 41.0 34.0 41.0 30-31 37.922274215229294 40.0 37.5 41.0 34.0 41.0 32-33 37.59896367232076 40.0 36.5 41.0 33.5 41.0 34-35 37.3802964769936 40.0 35.5 41.0 33.0 41.0 36-37 37.12364006924163 40.0 35.0 41.0 33.0 41.0 38-39 36.86285370287991 39.0 35.0 41.0 33.0 41.0 40-41 36.67092999922635 39.0 35.0 41.0 31.5 41.0 42-43 36.43935373672707 39.0 35.0 41.0 31.0 41.0 44-45 36.11969989652438 39.0 35.0 41.0 30.5 41.0 46-47 35.67674324990813 39.0 35.0 41.0 28.5 41.0 48-49 35.26406107962788 39.0 35.0 41.0 26.0 41.0 50-51 34.568565291182324 38.5 34.5 40.5 24.0 40.5 52-53 34.71766386863432 38.5 35.0 40.0 24.0 41.0 54-55 34.8983654236698 39.0 35.0 40.0 24.0 41.0 56-57 34.70421566446821 39.0 35.0 40.0 23.0 41.0 58-59 34.50211303019167 39.0 35.0 40.0 22.0 41.0 60-61 34.20378713517591 38.0 35.0 40.0 20.5 41.0 62-63 33.77433973850647 38.0 34.0 40.0 16.0 41.0 64-65 33.368886355724044 37.5 34.0 40.0 10.5 41.0 66-67 32.916217990252015 37.0 33.0 39.5 7.5 41.0 68-69 32.40388916020347 36.5 33.0 39.0 2.0 40.5 70-71 31.99508128155039 36.0 32.5 39.0 2.0 40.0 72-73 31.433080527242133 35.0 31.5 38.0 2.0 40.0 74-75 30.805407205578014 35.0 31.0 37.5 2.0 39.5 76-77 30.222651490242345 35.0 30.5 37.0 2.0 39.0 78-79 29.57380011798155 34.5 29.5 36.0 2.0 39.0 80-81 28.969678379397713 34.0 29.0 36.0 2.0 37.5 82-83 28.31058944838791 34.0 28.0 35.0 2.0 37.0 84-85 27.71279652534669 34.0 26.5 35.0 2.0 36.5 86-87 27.152291090458966 33.5 26.0 35.0 2.0 36.0 88-89 26.447987181594875 33.0 25.0 35.0 2.0 35.5 90-91 25.736328767189526 32.0 22.0 35.0 2.0 35.0 92-93 24.95891256310079 32.0 19.0 34.5 2.0 35.0 94-95 24.137698731214822 31.0 6.0 34.0 2.0 35.0 96-97 23.0653921194128 31.0 2.0 34.0 2.0 35.0 98-99 21.733138067423553 30.5 2.0 34.0 2.0 35.0 100-101 17.784434051215598 23.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2867127573647039 1101 2 0.024473022427834223 1101 3 -0.1078704506474466 1101 4 0.021749950932800743 1101 5 0.11588787827995617 1101 6 0.196074484591108 1101 7 0.014293332193354047 1101 8 -0.11134096148939676 1101 9 0.06551238041142682 1101 10-11 0.036054045233932186 1101 12-13 0.11858613882311886 1101 14-15 0.09655620357644068 1101 16-17 0.2574592560350055 1101 18-19 0.11286442355302029 1101 20-21 0.1841085856521758 1101 22-23 0.1057877711724231 1101 24-25 -0.056882310369154254 1101 26-27 0.16531289229168067 1101 28-29 0.20069561921212653 1101 30-31 0.2695873430221596 1101 32-33 0.09146899363350514 1101 34-35 0.25815958719076804 1101 36-37 0.1831081058658981 1101 38-39 0.3056196946631218 1101 40-41 0.2976964987604376 1101 42-43 0.5685597190814065 1101 44-45 0.4435542441173581 1101 46-47 0.4172510835483152 1101 48-49 0.8111223368414144 1101 50-51 0.3985207183882622 1101 52-53 0.5137785066946137 1101 54-55 0.5270446464589753 1101 56-57 0.6045771473845747 1101 58-59 0.5239003578898647 1101 60-61 0.5399864691678644 1101 62-63 0.4352714335190768 1101 64-65 0.45165252239768705 1101 66-67 0.3357550746205007 1101 68-69 0.4621560113602641 1101 70-71 0.6041235906666103 1101 72-73 0.5575437582002678 1101 74-75 0.6906745707275377 1101 76-77 0.6476748359110367 1101 78-79 0.6511621419588387 1101 80-81 0.8340411951700482 1101 82-83 0.8963056804854546 1101 84-85 1.2148204901867459 1101 86-87 1.195443877623621 1101 88-89 1.6397590046793908 1101 90-91 1.6656231118552434 1101 92-93 1.965505229826146 1101 94-95 2.198975325676443 1101 96-97 2.3369666509823546 1101 98-99 2.8231112747896603 1101 100-101 3.243708567188218 1102 1 0.39868811263593784 1102 2 0.28118107838993467 1102 3 0.32676042922917503 1102 4 0.3499961778972178 1102 5 0.39055621310834 1102 6 0.25994068543148074 1102 7 0.25946195816317186 1102 8 0.3335074089054544 1102 9 0.35936064064465967 1102 10-11 0.3357998866326781 1102 12-13 0.3857379100646412 1102 14-15 0.5631398555916078 1102 16-17 0.5984566908429301 1102 18-19 0.5046562023484782 1102 20-21 0.4631912540814298 1102 22-23 0.4165478076831306 1102 24-25 0.28242925817497877 1102 26-27 0.43221876705365503 1102 28-29 0.4379090022755676 1102 30-31 0.47682496164261323 1102 32-33 0.5124457337937116 1102 34-35 0.5345669019225241 1102 36-37 0.5036253146851237 1102 38-39 0.41896420153248215 1102 40-41 0.2890153694464388 1102 42-43 0.3552100975652692 1102 44-45 0.21484297773081806 1102 46-47 0.4077496435785335 1102 48-49 0.4202511944653864 1102 50-51 0.43433898318533437 1102 52-53 0.31317025716280256 1102 54-55 0.4043344344765387 1102 56-57 0.396835932773989 1102 58-59 0.5344994797443547 1102 60-61 0.5253310389867281 1102 62-63 0.4613600664133415 1102 64-65 0.42373245252374403 1102 66-67 0.39444367918748213 1102 68-69 0.5416336878942687 1102 70-71 0.63185481204426 1102 72-73 0.6985722743232046 1102 74-75 0.5697859598656052 1102 76-77 0.7451009882802992 1102 78-79 0.9317221564372211 1102 80-81 1.0583971431108132 1102 82-83 1.05233191468907 1102 84-85 1.1296950633104572 1102 86-87 1.093961416927712 1102 88-89 1.212272892912555 1102 90-91 1.4782086838301254 1102 92-93 2.062290084176741 1102 94-95 2.0065996902799412 1102 96-97 2.123953415850238 1102 98-99 2.11122916346347 1102 100-101 2.7433111984772687 1103 1 0.41569184680756166 1103 2 0.5006857309910657 1103 3 0.3330173755789403 1103 4 0.38181569138534144 1103 5 0.2940025326474043 1103 6 0.3120516782189071 1103 7 0.3561318955239514 1103 8 0.22393063052366102 1103 9 0.39537005280326554 1103 10-11 0.21565186834779126 1103 12-13 0.34974942414800125 1103 14-15 0.5150420509869065 1103 16-17 0.5863171453609084 1103 18-19 0.4104590492474429 1103 20-21 0.6654829100912139 1103 22-23 0.5654368567481995 1103 24-25 0.6335681107658857 1103 26-27 0.593345017396615 1103 28-29 0.5547367235008167 1103 30-31 0.6176957723252272 1103 32-33 0.6522400055837423 1103 34-35 0.7594635495385589 1103 36-37 0.8378964536772244 1103 38-39 0.713120897232379 1103 40-41 0.5465116143485673 1103 42-43 0.6503073115849247 1103 44-45 0.6927030137239498 1103 46-47 0.7400560409632178 1103 48-49 0.6198631289337015 1103 50-51 0.5973172059428506 1103 52-53 0.6366504307111001 1103 54-55 0.8227953326308466 1103 56-57 0.850611026825824 1103 58-59 1.067638509045743 1103 60-61 0.9848050462690665 1103 62-63 0.7126923704855841 1103 64-65 0.8166075321597219 1103 66-67 0.7992629927137429 1103 68-69 1.1121423350824813 1103 70-71 1.3767778732279439 1103 72-73 1.2905698468791371 1103 74-75 1.422224564596796 1103 76-77 1.5868625593295391 1103 78-79 1.6408636689859897 1103 80-81 1.7858700436274582 1103 82-83 1.787637042548404 1103 84-85 1.8447180124224865 1103 86-87 1.708085002662525 1103 88-89 2.56061344192414 1103 90-91 2.9305526554448385 1103 92-93 3.0657429141021986 1103 94-95 3.2005115489487537 1103 96-97 3.3075579377786184 1103 98-99 3.4821793543572994 1103 100-101 3.652740098082461 1104 1 0.14848525827307668 1104 2 0.09280753130138208 1104 3 0.1576431179364235 1104 4 0.08894632314445516 1104 5 0.033669527028536095 1104 6 0.05140288778002855 1104 7 -0.04401168130754485 1104 8 0.128772032860887 1104 9 0.1283824084106584 1104 10-11 0.05068061529257051 1104 12-13 0.1654679293489778 1104 14-15 0.15684627702989218 1104 16-17 0.2222856679300449 1104 18-19 0.1388483150898452 1104 20-21 0.14721265302131314 1104 22-23 0.04282245317098443 1104 24-25 -0.09620323997210534 1104 26-27 0.16175243034000175 1104 28-29 0.2807249720950509 1104 30-31 0.29262793121973374 1104 32-33 0.31589654117163235 1104 34-35 0.2479626663936827 1104 36-37 0.4099259185061612 1104 38-39 0.3541588187787923 1104 40-41 0.4452615275077463 1104 42-43 0.3941300341974312 1104 44-45 0.5295254184464042 1104 46-47 0.4626643393672438 1104 48-49 0.7518706572438063 1104 50-51 0.9635886503636755 1104 52-53 0.7353268712688177 1104 54-55 0.7516342760569401 1104 56-57 0.978354407449693 1104 58-59 0.899608353700664 1104 60-61 0.9729094320991649 1104 62-63 0.8696584783534647 1104 64-65 0.7922626277688423 1104 66-67 0.9655489632362801 1104 68-69 1.1012509166402253 1104 70-71 1.1003324734759374 1104 72-73 1.1561528803923267 1104 74-75 1.1587916046614666 1104 76-77 1.1285598395801486 1104 78-79 1.4191416733101399 1104 80-81 1.278233314063474 1104 82-83 1.3354331802895345 1104 84-85 1.5962574634200486 1104 86-87 1.7640473358491953 1104 88-89 2.1152347422418174 1104 90-91 2.0022684406858993 1104 92-93 2.428529146726138 1104 94-95 2.5018523712945253 1104 96-97 2.824961891825609 1104 98-99 3.108903802628589 1104 100-101 3.743802966629662 1105 1 0.28884436743046393 1105 2 0.1698201308374081 1105 3 0.13559122877776275 1105 4 0.0209183982631842 1105 5 0.18358594686489482 1105 6 0.15021115658247908 1105 7 0.29365526678940057 1105 8 0.0528846399600269 1105 9 0.24717119832732948 1105 10-11 0.11429260576613132 1105 12-13 0.09720693697538252 1105 14-15 0.17223181851050384 1105 16-17 0.38538352077647886 1105 18-19 0.3793770541638395 1105 20-21 0.2742637627158473 1105 22-23 0.30917846608075905 1105 24-25 0.25146554488190276 1105 26-27 0.5491669401033619 1105 28-29 0.39705960762225345 1105 30-31 0.3833976867766964 1105 32-33 0.5236576886663116 1105 34-35 0.3404348950899916 1105 36-37 0.06962191206053348 1105 38-39 0.12925100391773725 1105 40-41 0.25297158537330944 1105 42-43 0.3452221329007443 1105 44-45 0.3955247300483862 1105 46-47 0.4027343522245417 1105 48-49 0.13861837789004028 1105 50-51 0.11935413816159013 1105 52-53 0.08219921356624837 1105 54-55 0.07703894141987178 1105 56-57 0.11635891753719108 1105 58-59 0.06527226420274701 1105 60-61 0.1795870472784955 1105 62-63 0.11253564252405113 1105 64-65 0.2660857526945577 1105 66-67 0.5044928433624207 1105 68-69 0.6000485353387148 1105 70-71 0.5841481741987522 1105 72-73 0.5041990493647148 1105 74-75 0.44563363274995993 1105 76-77 0.4101308379579365 1105 78-79 0.7370480480919248 1105 80-81 0.6019169843671257 1105 82-83 0.575121487247138 1105 84-85 0.6507952566259014 1105 86-87 0.7571672143453974 1105 88-89 0.7179820060140223 1105 90-91 0.9872965554168438 1105 92-93 1.476469277706979 1105 94-95 1.8141280824204884 1105 96-97 2.0078550465870535 1105 98-99 2.526560748888784 1105 100-101 3.131701361550949 1106 1 0.13687980430081836 1106 2 0.016928748199234178 1106 3 0.13372287833995244 1106 4 0.10569443159338476 1106 5 0.08899739588100175 1106 6 -0.11184191045452252 1106 7 0.058506446056888706 1106 8 0.07588741747627381 1106 9 0.016702080541804776 1106 10-11 0.045065073047929616 1106 12-13 0.2291698524259047 1106 14-15 0.44101241826570714 1106 16-17 0.15982507524404355 1106 18-19 0.28437952568253166 1106 20-21 0.3069797557955951 1106 22-23 0.23181993110288346 1106 24-25 0.2013613880228533 1106 26-27 0.05030854635513293 1106 28-29 0.1630251770130826 1106 30-31 0.028815667663614875 1106 32-33 0.23959412944402203 1106 34-35 0.1390605541616523 1106 36-37 0.2176379298554636 1106 38-39 -0.12619663519345892 1106 40-41 0.08749237555314693 1106 42-43 0.0066674711457324065 1106 44-45 -0.0775541055132365 1106 46-47 0.13408615525627 1106 48-49 0.15739162193485612 1106 50-51 0.24467116927918653 1106 52-53 0.26659657492582056 1106 54-55 -0.2816254969569272 1106 56-57 0.048854092191177756 1106 58-59 0.1368403839654846 1106 60-61 0.16501023600837073 1106 62-63 0.20350739071160007 1106 64-65 0.29158285160238506 1106 66-67 0.33252957483779966 1106 68-69 -0.10214008272925668 1106 70-71 0.07984067019724961 1106 72-73 0.08315083121199507 1106 74-75 0.09705019545592108 1106 76-77 0.32087738371633634 1106 78-79 0.5911644507376295 1106 80-81 0.5371492188554008 1106 82-83 0.838772950150819 1106 84-85 0.9984879173923069 1106 86-87 0.9218637765805973 1106 88-89 1.1980590644360163 1106 90-91 1.177896987344159 1106 92-93 1.3016770029682583 1106 94-95 1.6348422042075157 1106 96-97 2.0745875223426324 1106 98-99 2.2909852969539344 1106 100-101 2.864576532203433 1107 1 0.30557552390379783 1107 2 0.2814570626267425 1107 3 0.10070045721269594 1107 4 0.1587230561945887 1107 5 0.23615265997174362 1107 6 0.37429443304068855 1107 7 0.2303039026325706 1107 8 0.2935185766396913 1107 9 0.33593728082539087 1107 10-11 0.2589575313960353 1107 12-13 0.28683446603810125 1107 14-15 0.40947439983252565 1107 16-17 0.5499460429859795 1107 18-19 0.43994271249571426 1107 20-21 0.49125339546237967 1107 22-23 0.563750097710475 1107 24-25 0.5013104309154457 1107 26-27 0.44148188914137876 1107 28-29 0.4047363388351286 1107 30-31 0.5561608023676214 1107 32-33 0.631423821928955 1107 34-35 0.6197577803225869 1107 36-37 0.564561671436465 1107 38-39 0.5707138132290837 1107 40-41 0.6943222895907226 1107 42-43 0.7126144979092857 1107 44-45 0.6435138889922527 1107 46-47 0.7062458503998315 1107 48-49 0.6125881391273467 1107 50-51 0.15253019533377454 1107 52-53 0.32358545298324515 1107 54-55 0.5233638685163058 1107 56-57 0.6650252194510173 1107 58-59 0.5766223761668314 1107 60-61 0.5718279646507014 1107 62-63 0.5731818191057911 1107 64-65 0.5792495699646665 1107 66-67 0.7858872051107539 1107 68-69 0.7715333708333105 1107 70-71 0.8858934883468379 1107 72-73 0.8445663310347555 1107 74-75 1.033683370748367 1107 76-77 1.195752760355827 1107 78-79 1.3724449142257527 1107 80-81 1.3978673817409053 1107 82-83 1.3996805405442956 1107 84-85 1.4942894948471093 1107 86-87 1.733657577537702 1107 88-89 1.8154958111570387 1107 90-91 2.1379538155965534 1107 92-93 2.4273305832589323 1107 94-95 2.49216507287111 1107 96-97 2.839736184164682 1107 98-99 3.236588584795129 1107 100-101 3.447550765631295 1108 1 0.35174011623298185 1108 2 0.1835580096857825 1108 3 0.07770433979188596 1108 4 0.23932286025134175 1108 5 0.2945546339185512 1108 6 0.21665083203919977 1108 7 0.13989786467764276 1108 8 0.14789464253468054 1108 9 0.111736475280928 1108 10-11 0.14053886598727416 1108 12-13 0.2379348325245445 1108 14-15 0.26683497169653236 1108 16-17 0.38708182440039707 1108 18-19 0.2935703840367765 1108 20-21 0.2767998594124492 1108 22-23 0.27505130602717287 1108 24-25 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-2.3279357750948826 2316 82-83 -1.9653577008354688 2316 84-85 -1.9320755313134228 2316 86-87 -1.7000053799421018 2316 88-89 -2.0017895780440007 2316 90-91 -2.05617609966178 2316 92-93 -2.1244270705083146 2316 94-95 -2.7297535417410046 2316 96-97 -3.1640566534291636 2316 98-99 -3.4234370925012207 2316 100-101 -3.5950226969158763 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4650.0 3 699.0 4 570.0 5 523.0 6 541.0 7 590.0 8 683.0 9 876.0 10 1137.0 11 1741.0 12 2773.0 13 4051.0 14 5092.0 15 6886.0 16 9098.0 17 12279.0 18 10872.0 19 6472.0 20 6030.0 21 6117.0 22 6267.0 23 6943.0 24 7619.0 25 8598.0 26 9891.0 27 11523.0 28 13234.0 29 15678.0 30 19282.0 31 23647.0 32 29965.0 33 39366.0 34 56790.0 35 90162.0 36 110876.0 37 167423.0 38 119789.0 39 8515.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.35837731880133 24.39669264906276 15.855293680423815 19.389636351712095 2 13.930207471689716 24.386146947423413 41.69218341097196 19.99146216991491 3 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16.232850450729405 22-23 17.659174331343845 31.036832780025275 34.640349763256715 16.663643125374165 24-25 17.052145307938375 32.33798980161474 34.68660619729418 15.923258693152711 26-27 16.300731708644612 32.1838107270096 35.28415320705377 16.23130435729202 28-29 16.22485020326641 31.2428608832943 35.9934838647648 16.53880504867449 30-31 16.776441107000448 31.71179190345977 35.15342016116645 16.358346828373328 32-33 16.114217228247004 32.55303478327123 35.06898966334205 16.263758325139715 34-35 16.76486364397765 31.857607025432845 35.039237468168885 16.338291862420622 36-37 17.302747744479113 31.151283167646277 34.329148828096656 17.216820259777954 38-39 16.2444128581197 32.49976874537869 34.620056214522066 16.63576218197955 40-41 16.002415860523353 31.707564799291927 35.91615585565052 16.3738634845342 42-43 16.217438434783766 31.640709309643732 34.75613626841606 17.385715987156445 44-45 16.21721121202449 31.703022081652787 34.626386752942764 17.45337995337995 46-47 16.974854642287323 31.591217292625313 34.07957446782783 17.354353597259532 48-49 16.576928425036424 31.797747374657664 33.8356296891909 17.789694511115005 50-51 16.397605867691606 31.594276667244987 33.83298744839685 18.175130016666554 52-53 16.452064532977396 32.023413999555075 34.06086479219259 17.463656675274933 54-55 17.00309211977959 31.570573175634216 33.62316037008714 17.80317433449905 56-57 16.9693177730827 32.11601877627757 32.957445072133915 17.957218378505818 58-59 16.515811255125488 30.985901018780304 33.93766174414366 18.560625981950547 60-61 17.12252248548826 32.04485853396672 33.01440352851991 17.818215452025115 62-63 16.849997098309284 31.63247910782683 33.86526966379077 17.65225413007312 64-65 16.991819590907003 30.964408087070698 33.701810426334 18.341961895688296 66-67 16.809092354348905 31.70361352416381 32.77354406330603 18.71375005818125 68-69 16.659946671244725 31.06064498657109 33.47845299458914 18.80095534759504 70-71 17.20072520323041 30.970155795368882 33.684347979061236 18.144771022339476 72-73 17.22506347479144 30.511123201547573 33.2864828920324 18.97733043162858 74-75 16.788565176258654 31.405626131558506 32.715528836417825 19.090279855765015 76-77 16.85667447250166 30.842860925166743 33.48008061327991 18.82038398905168 78-79 16.820502280482756 30.43194670712798 33.199174250644546 19.548376761744713 80-81 17.085166796538026 30.984021893349116 32.38367042457356 19.547140885539303 82-83 17.52504467297448 30.517988881930357 32.51799613599928 19.438970309095875 84-85 16.834152628231944 30.213121453114006 32.68376103509226 20.268964883561793 86-87 16.95957757001211 30.45354648253052 33.11138351140092 19.47549243605645 88-89 17.64123331376441 29.72666587262384 33.19254188223216 19.439558931379587 90-91 17.281692635952552 29.57687124268641 33.4409147605628 19.70052136079824 92-93 17.87504329916352 29.833410813395567 32.913469984385095 19.378075903055816 94-95 17.541737105211606 28.691463452976073 33.477293998998746 20.289505442813578 96-97 18.053293382039087 28.624908858087057 32.84567119746266 20.476126562411203 98-99 17.592658087801873 28.911862593066175 32.808807392696174 20.686671926435782 100-101 18.13898697227924 27.92106730333263 33.658627830688665 20.281317893699466 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7649.0 1 4469.5 2 1775.0 3 2138.0 4 1556.0 5 1575.0 6 3332.0 7 6217.0 8 8435.5 9 8323.0 10 5974.5 11 3953.5 12 3478.0 13 3283.0 14 2944.5 15 2617.0 16 2387.5 17 2327.0 18 2358.5 19 2501.0 20 2788.0 21 3081.0 22 3301.5 23 3539.5 24 3869.5 25 4782.0 26 7177.5 27 9590.5 28 9791.0 29 10843.5 30 14787.5 31 18572.0 32 23647.0 33 30436.5 34 36698.5 35 41553.0 36 48384.0 37 54431.5 38 52415.5 39 48144.5 40 43537.5 41 37695.0 42 32393.0 43 31043.5 44 30666.0 45 27870.0 46 23337.0 47 18807.0 48 19094.5 49 17061.5 50 11400.0 51 7227.5 52 4602.5 53 3346.0 54 2696.5 55 2210.0 56 1883.0 57 1644.5 58 1458.5 59 1290.0 60 1121.0 61 994.5 62 826.5 63 628.0 64 464.0 65 401.5 66 352.5 67 298.0 68 247.0 69 219.0 70 201.5 71 184.5 72 167.5 73 138.5 74 109.0 75 84.0 76 70.0 77 52.5 78 33.5 79 25.5 80 21.0 81 15.5 82 9.5 83 4.5 84 2.5 85 2.5 86 2.5 87 1.5 88 0.5 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4678161808792526 2 0.04110012958629093 3 0.052221341121404946 4 0.056452236814111365 5 0.08256290737481384 6 0.08352996924743245 7 0.04714426629015724 8 0.04206719145890954 9 0.04641896988569329 10-11 0.05161692745101831 12-13 0.047386031758311895 14-15 0.04085836411813628 16-17 0.041583660522600235 18-19 0.04913883140243313 20-21 0.057661064154884624 22-23 0.04871574183316249 24-25 0.06630417964141345 26-27 0.05856768466046458 28-29 0.04563323211419067 30-31 0.04847397636500783 32-33 0.04653985261977061 34-35 0.051193837881747674 36-37 0.04690250082200259 38-39 0.028709649343364986 40-41 0.025808463725509156 42-43 0.02641287739589579 44-45 0.016440051834516373 46-47 0.028588766609287664 48-49 0.025748022358470492 50-51 0.017225789606018994 52-53 0.016440051834516373 54-55 0.01758843780825097 56-57 0.016984024137864342 58-59 0.015896079531168405 60-61 0.019220354718294877 62-63 0.017407113707134984 64-65 0.01813241011159894 66-67 0.011060770168075353 68-69 0.013841073051853859 70-71 0.0204896234261068 72-73 0.017890644643444288 74-75 0.015170783126704446 76-77 0.010758563332882038 78-79 0.014566369456317816 80-81 0.017830203276405627 82-83 0.015231224493743109 84-85 0.013780631684815194 86-87 0.004110012958629093 88-89 0.031006421290834186 90-91 0.033544958706458045 92-93 0.018555499680869583 94-95 0.03457246194611531 96-97 0.029132738912635632 98-99 0.02605022919366381 100-101 0.15454857551786164 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 827248.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.298225884213437 #Duplication Level Percentage of deduplicated Percentage of total 1 75.85892119162992 14.6394259648882 2 9.052471087516313 3.493932637144022 3 3.5853588758136894 2.075731963842664 4 1.9066932502146594 1.4718318813819038 5 1.2230245299228435 1.1801101820192494 6 0.8541453747602229 0.9890094228047352 7 0.6698978946297698 0.9049488622947016 8 0.5360697624518183 0.8276156292393464 9 0.40965153458067854 0.7114993063337347 >10 4.163512945660786 17.740932028903313 >50 0.8562952814089207 11.631183444174123 >100 0.8210878395888769 30.07242995138532 >500 0.0509250497754204 6.608171973994091 >1k 0.009430564773225998 2.7842528313452015 >5k 0.0012574086364301332 1.9116562493757625 >10k+ 0.0012574086364301332 2.957267670873625 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14100 1.7044465504903004 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10274 1.24194920991045 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8009 0.9681498172253061 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7747 0.9364785408970465 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3060 0.3699011662766184 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2524 0.3051080208111715 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2317 0.28008529485716493 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1776 0.21468773572133146 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1712 0.2069512407403826 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1496 0.18084057017968008 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1244 0.15037812119219388 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1243 0.15025723845811656 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1219 0.1473560528402607 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1111 0.13430071755990947 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1096 0.1324874765487496 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1073 0.1297071736649711 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1035 0.12511362977003268 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1035 0.12511362977003268 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1007 0.12172891321586755 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 975 0.11786066572539312 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 956 0.1155638937779239 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 937 0.11326712183045472 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 936 0.11314623909637739 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 897 0.10843181246736167 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 896 0.10831092973328434 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 887 0.1072229851265884 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 887 0.1072229851265884 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 881 0.10649768872212444 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 880 0.10637680598804711 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 871 0.10528886138135118 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 854 0.10323385490203663 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 837 0.10117884842272208 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 837 0.10117884842272208 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 836 0.10105796568864475 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 831 0.10045355201825812 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.2088273407732627E-4 0.0 14-15 0.0 0.0 0.0 1.813241011159894E-4 0.0 16-17 0.0 0.0 0.0 2.4176546815465254E-4 0.0 18-19 0.0 0.0 0.0 3.0220683519331567E-4 0.0 20-21 0.0 0.0 0.0 8.461791385412839E-4 0.0 22-23 0.0 0.0 0.0 0.0020550064793145462 0.0 24-25 0.0 0.0 0.0 0.005923253969788987 0.0 26-27 0.0 0.0 0.0 0.012874011179235248 0.0 28-29 0.0 0.0 0.0 0.02943494574782895 0.0 30-31 0.0 0.0 0.0 0.07404067462236234 0.0 32-33 0.0 0.0 0.0 0.15013635572403922 0.0 34-35 0.0 0.0 0.0 0.2649749530974992 0.0 36-37 0.0 0.0 0.0 0.43819991103030775 0.0 38-39 0.0 0.0 0.0 0.672833297874398 0.0 40-41 0.0 0.0 0.0 0.9239067365530047 0.0 42-43 0.0 0.0 0.0 1.1619852813182987 0.0 44-45 0.0 0.0 0.0 1.4324603988163163 0.0 46-47 0.0 0.0 0.0 1.7487500725296403 0.0 48-49 0.0 0.0 0.0 2.0600231127787554 0.0 50-51 0.0 0.0 0.0 2.384351488308222 0.0 52-53 0.0 0.0 0.0 2.6999763069841207 0.0 54-55 0.0 0.0 0.0 3.0270245440303274 0.0 56-57 0.0 0.0 0.0 3.4043599984527013 0.0 58-59 0.0 0.0 0.0 3.7977728565073594 0.0 60-61 0.0 0.0 0.0 4.20236736746417 0.0 62-63 0.0 0.0 0.0 4.572449857841905 0.0 64-65 0.0 0.0 0.0 4.97281347310601 0.0 66-67 0.0 0.0 0.0 5.39868334526043 0.0 68-69 0.0 0.0 0.0 5.845647254511343 0.0 70-71 0.0 0.0 0.0 6.32869465988434 0.0 72-73 0.0 0.0 0.0 6.787505077074831 0.0 74-75 0.0 0.0 0.0 7.257497147167475 0.0 76-77 0.0 0.0 0.0 7.753841653288978 0.0 78-79 0.0 0.0 0.0 8.297934839371022 0.0 80-81 0.0 0.0 0.0 8.87944123165 0.0 82-83 0.0 0.0 0.0 9.425589424211362 0.0 84-85 0.0 0.0 0.0 9.973309092315727 0.0 86-87 0.0 0.0 0.0 10.533056592460785 0.0 88-89 0.0 0.0 0.0 11.11111782681856 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 125 1.2732926E-10 38.19013 1 GGTCGAT 25 0.0016165901 37.997276 34-35 GGTATCA 5360 0.0 37.851692 1 ATTGCCG 80 1.076208E-5 35.629993 8 GCCGTGT 40 1.0795402E-5 35.617065 92-93 GTATCAA 8600 0.0 34.804085 1 CCTTTAG 215 0.0 33.305347 1 TACCTGG 200 0.0 33.274784 2 GTGGTAC 130 8.230927E-9 32.905148 6 CATTCCG 225 0.0 31.671104 9 GAGTGGG 105 1.898743E-6 31.6711 7 GGTAATC 105 1.898743E-6 31.6711 8 ATGTATT 125 2.0605148E-7 30.552103 1 TAGGCAT 265 0.0 30.494415 5 GGAGTGG 80 4.4761685E-4 29.706036 6 CTTGTCA 65 0.0058391364 29.234863 9 TGGTACT 150 3.32966E-8 28.503994 7 TCAACGC 10410 0.0 28.40252 4 ATCAACG 10415 0.0 28.388885 3 CATGGGT 540 0.0 28.169132 4 >>END_MODULE