##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938213_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 835183 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07670295013189 33.0 31.0 34.0 31.0 34.0 2 32.24839586054793 34.0 31.0 34.0 31.0 34.0 3 32.285381766630785 34.0 31.0 34.0 30.0 34.0 4 35.803534075765434 37.0 35.0 37.0 35.0 37.0 5 35.853305203769715 37.0 35.0 37.0 35.0 37.0 6 35.918221515524145 37.0 37.0 37.0 35.0 37.0 7 35.87494118055564 37.0 37.0 37.0 35.0 37.0 8 35.878923541307714 37.0 37.0 37.0 35.0 37.0 9 37.56009760735073 39.0 38.0 39.0 35.0 39.0 10-11 37.51296482327825 39.0 38.0 39.0 35.0 39.0 12-13 37.461570697679434 39.0 38.0 39.0 35.0 39.0 14-15 38.71683032341415 40.0 38.0 41.0 35.5 41.0 16-17 38.67819208484847 40.0 38.0 41.0 35.5 41.0 18-19 38.72954849416236 40.0 38.5 41.0 35.0 41.0 20-21 38.68606940035896 40.0 38.5 41.0 35.0 41.0 22-23 38.54319711967317 40.0 38.0 41.0 35.0 41.0 24-25 38.48426512512827 40.0 38.0 41.0 35.0 41.0 26-27 38.4245602460778 40.0 38.0 41.0 35.0 41.0 28-29 38.31857209737267 40.0 38.0 41.0 35.0 41.0 30-31 38.07367906195409 40.0 37.5 41.0 34.0 41.0 32-33 37.762452660075695 40.0 36.5 41.0 34.0 41.0 34-35 37.5606028858346 40.0 36.0 41.0 33.5 41.0 36-37 37.333480806002996 40.0 35.5 41.0 33.0 41.0 38-39 37.1070316325883 40.0 35.0 41.0 33.0 41.0 40-41 36.92195243437666 40.0 35.0 41.0 33.0 41.0 42-43 36.626639910055644 40.0 35.0 41.0 31.5 41.0 44-45 36.297907763927185 40.0 35.0 41.0 30.5 41.0 46-47 35.98708546510166 40.0 35.0 41.0 30.0 41.0 48-49 35.677330597006886 40.0 35.0 41.0 27.5 41.0 50-51 35.037906063701016 39.0 35.0 40.5 26.0 41.0 52-53 35.13300318612807 39.0 35.0 40.0 26.5 41.0 54-55 35.32030764515082 40.0 35.0 41.0 26.0 41.0 56-57 35.19024752658998 39.5 35.0 41.0 24.5 41.0 58-59 35.0234894627884 39.0 35.0 41.0 24.0 41.0 60-61 34.75652521662917 39.0 35.0 41.0 22.5 41.0 62-63 34.41216715378546 39.0 35.0 40.5 21.0 41.0 64-65 34.07621682912607 38.0 35.0 40.0 19.0 41.0 66-67 33.69912821501396 38.0 34.5 40.0 14.0 41.0 68-69 33.25610494945419 37.0 34.0 39.5 8.0 41.0 70-71 32.82378831944616 36.5 34.0 39.0 4.0 41.0 72-73 32.349053440982395 36.0 33.0 39.0 2.0 40.0 74-75 31.81899176587646 35.5 33.0 38.0 2.0 40.0 76-77 31.315876281006677 35.0 32.0 37.0 2.0 39.0 78-79 30.772658806513064 35.0 31.5 37.0 2.0 39.0 80-81 30.246566920064225 35.0 31.0 36.0 2.0 38.5 82-83 29.733651187823504 35.0 30.5 36.0 2.0 37.0 84-85 29.241365664770477 34.0 30.0 35.0 2.0 37.0 86-87 28.81371447934165 34.0 29.5 35.0 2.0 36.5 88-89 28.300234798840492 34.0 29.0 35.0 2.0 36.0 90-91 27.766576905899665 34.0 28.0 35.0 2.0 36.0 92-93 27.262524500618426 34.0 26.5 35.0 2.0 35.0 94-95 26.795532835318724 34.0 25.5 35.0 2.0 35.0 96-97 26.11696179160735 33.0 24.5 35.0 2.0 35.0 98-99 25.298893176704986 33.0 19.0 35.0 2.0 35.0 100-101 22.60698792959148 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4776541141903934 1101 2 0.4025084736086768 1101 3 0.5034431846147598 1101 4 0.3848782169365279 1101 5 0.31123330265638316 1101 6 0.3287986458292451 1101 7 0.4101518336394321 1101 8 0.4184792927911474 1101 9 0.5604774192284623 1101 10-11 0.44914178618188316 1101 12-13 0.32801467650143934 1101 14-15 0.4834060403547795 1101 16-17 0.5058558629849941 1101 18-19 0.4555568134857211 1101 20-21 0.5992893154198029 1101 22-23 0.5374712512408237 1101 24-25 0.578481361472555 1101 26-27 0.7137425211946038 1101 28-29 0.68149818597729 1101 30-31 0.7604392443297812 1101 32-33 0.7678818505681662 1101 34-35 0.5907017515548176 1101 36-37 0.5898334340828555 1101 38-39 0.713353083339392 1101 40-41 0.8568793313712391 1101 42-43 0.9379669947953957 1101 44-45 0.6806851179137823 1101 46-47 0.8640487372991501 1101 48-49 0.7044817912631132 1101 50-51 0.8833595777476546 1101 52-53 0.8795760650905606 1101 54-55 0.8226682739133793 1101 56-57 0.6446421248496321 1101 58-59 0.737744418336483 1101 60-61 0.7782919011317304 1101 62-63 0.5885861087687587 1101 64-65 0.7194843261619539 1101 66-67 0.8347931550908427 1101 68-69 0.7196228616319402 1101 70-71 0.9700934089275961 1101 72-73 0.8339471268386802 1101 74-75 0.9800992677070504 1101 76-77 0.827994693203685 1101 78-79 0.9307578869033541 1101 80-81 1.1663110086175479 1101 82-83 1.0843203211302246 1101 84-85 1.141861446978922 1101 86-87 1.2306582681232037 1101 88-89 1.0851877873951672 1101 90-91 1.1797338818333358 1101 92-93 1.372338437145462 1101 94-95 1.449382343123549 1101 96-97 1.384085492155485 1101 98-99 1.4892653512004905 1101 100-101 1.81931119801812 1102 1 0.3061025375239197 1102 2 0.22161094267408288 1102 3 0.07567712808666016 1102 4 0.17912656804181637 1102 5 0.13206544802522302 1102 6 0.14509941943172322 1102 7 -0.029085208043866828 1102 8 0.11390040244502586 1102 9 0.14732237560640726 1102 10-11 0.32349125682490865 1102 12-13 0.12409344329925887 1102 14-15 0.2234052800574986 1102 16-17 0.21558196589123213 1102 18-19 0.3695919012050197 1102 20-21 0.2771694165393441 1102 22-23 0.267928760126793 1102 24-25 0.17733331258543927 1102 26-27 0.2895527129795923 1102 28-29 0.3881203752533011 1102 30-31 0.24855199641584846 1102 32-33 0.37339875767884934 1102 34-35 0.32833456598525856 1102 36-37 0.30881615632725357 1102 38-39 0.33448554613234904 1102 40-41 0.5587563152719426 1102 42-43 0.32814300361484783 1102 44-45 0.17655100147425884 1102 46-47 0.1517566310856182 1102 48-49 0.25431072437596214 1102 50-51 0.2604432674703361 1102 52-53 0.3050517260740051 1102 54-55 0.12337820149506484 1102 56-57 0.18597283515736507 1102 58-59 0.12486023061908469 1102 60-61 0.08074861170555891 1102 62-63 0.12140814218382445 1102 64-65 0.16928569849853403 1102 66-67 0.16872807364399733 1102 68-69 0.5093883099222225 1102 70-71 0.40880679586188506 1102 72-73 0.2945380678966316 1102 74-75 0.20723142638108882 1102 76-77 0.20054511041681522 1102 78-79 0.2262882892906859 1102 80-81 0.3442481320328028 1102 82-83 0.6133579748528284 1102 84-85 0.40913540109498214 1102 86-87 0.2871977750704211 1102 88-89 0.1432231792333738 1102 90-91 0.22050577364303692 1102 92-93 0.41971161005606916 1102 94-95 0.46633602201199054 1102 96-97 0.5211338280548219 1102 98-99 0.5622805001237197 1102 100-101 0.683085883920306 1103 1 0.21946286141055538 1103 2 0.15916154996153153 1103 3 0.1280725396925959 1103 4 -0.007783013604608868 1103 5 -0.10231376924873103 1103 6 0.09182681484332988 1103 7 -0.0481135481248387 1103 8 -0.044196763546878515 1103 9 0.042464076011263785 1103 10-11 0.14668626357254766 1103 12-13 -0.030528688954454708 1103 14-15 0.20768328275655534 1103 16-17 0.0991008592785434 1103 18-19 0.17331997138045807 1103 20-21 0.22583675796983727 1103 22-23 0.24114063597025392 1103 24-25 0.31211738815898826 1103 26-27 0.21929292890401797 1103 28-29 0.10040107700768175 1103 30-31 0.1798610382512038 1103 32-33 0.21601009371124036 1103 34-35 0.41342228528350233 1103 36-37 0.4030469255580229 1103 38-39 0.4681899995736458 1103 40-41 0.4125687309264663 1103 42-43 0.14278537878353603 1103 44-45 0.20398015127182845 1103 46-47 0.3841284259574138 1103 48-49 0.6444929106107793 1103 50-51 0.5909763308981439 1103 52-53 0.7513236558985668 1103 54-55 0.7557668654626752 1103 56-57 0.6178722953462952 1103 58-59 0.7104371536960059 1103 60-61 0.7502155482777653 1103 62-63 0.7452947818599398 1103 64-65 0.778361272047789 1103 66-67 0.5148987888936674 1103 68-69 0.6848100373176393 1103 70-71 0.7532568633382724 1103 72-73 0.8203815226874553 1103 74-75 0.7124102387967461 1103 76-77 0.8255113722251792 1103 78-79 0.7821756037306322 1103 80-81 1.0332494815604676 1103 82-83 1.1033714701294848 1103 84-85 1.0931772364526076 1103 86-87 1.0752375861365486 1103 88-89 0.9735032062239277 1103 90-91 1.1704214281639516 1103 92-93 1.2456056721343423 1103 94-95 0.986697020662465 1103 96-97 1.192304543304484 1103 98-99 1.254554454239777 1103 100-101 1.350056194311673 1104 1 0.4543715617327919 1104 2 0.35501278519246426 1104 3 0.34959509608357564 1104 4 0.33459077801730075 1104 5 0.308991279086392 1104 6 0.28493905974129063 1104 7 0.30732415327150875 1104 8 0.2907522160449574 1104 9 0.06442970436786766 1104 10-11 0.38783825068318123 1104 12-13 0.33767082769431056 1104 14-15 0.38698645139243126 1104 16-17 0.49471041889186296 1104 18-19 0.5151231400163354 1104 20-21 0.4373767687109762 1104 22-23 0.5308224297403896 1104 24-25 0.503779579351253 1104 26-27 0.34573626939810964 1104 28-29 0.40415376229231725 1104 30-31 0.4262627568333741 1104 32-33 0.26810461573057154 1104 34-35 0.41669161933291576 1104 36-37 0.3908825861380407 1104 38-39 0.3120272713244461 1104 40-41 0.3326949393045453 1104 42-43 0.4205176559049164 1104 44-45 0.2668359514866552 1104 46-47 0.34428551510886507 1104 48-49 0.2329059073884352 1104 50-51 0.18551177665538887 1104 52-53 0.17496087394368232 1104 54-55 0.10410870219737234 1104 56-57 0.05643969599076826 1104 58-59 0.09828624069923109 1104 60-61 0.04336807244532537 1104 62-63 -0.027885937796348514 1104 64-65 0.06446492403490112 1104 66-67 0.059158992975298474 1104 68-69 0.21748323817693205 1104 70-71 0.11058097719433846 1104 72-73 0.11328565802579504 1104 74-75 0.31130255888267655 1104 76-77 0.21973801024881112 1104 78-79 0.14679697859636676 1104 80-81 0.05091865449279709 1104 82-83 0.16351513285772867 1104 84-85 0.23140226330545133 1104 86-87 0.17415675906887884 1104 88-89 0.18837296991887698 1104 90-91 0.1369090758546072 1104 92-93 0.2438065314254274 1104 94-95 0.14120829885795416 1104 96-97 0.3668211920692528 1104 98-99 0.686072982703795 1104 100-101 0.553829019231113 1105 1 0.2641148138723537 1105 2 0.23107940290655193 1105 3 0.2598441212519518 1105 4 0.1553357012967851 1105 5 0.176186296050993 1105 6 0.2715414550418416 1105 7 0.28441877523940917 1105 8 0.34153524637867605 1105 9 0.30260512341886425 1105 10-11 0.41219005095663874 1105 12-13 0.2507857945247167 1105 14-15 0.254901514439986 1105 16-17 0.3074477967214122 1105 18-19 0.4422813792425373 1105 20-21 0.3220412114111397 1105 22-23 0.28932530359464437 1105 24-25 0.40103928968699876 1105 26-27 0.34284817247199584 1105 28-29 0.4485988045772231 1105 30-31 0.5097944325309527 1105 32-33 0.5394559602385982 1105 34-35 0.6114632935112709 1105 36-37 0.7007777600885206 1105 38-39 0.7124077088592671 1105 40-41 0.7704797927000016 1105 42-43 0.8773354636731838 1105 44-45 1.0247944388518206 1105 46-47 1.0408232803390263 1105 48-49 0.8080850485238003 1105 50-51 0.7796305035506208 1105 52-53 0.8910079317246087 1105 54-55 0.8524169895321521 1105 56-57 0.8856811630490498 1105 58-59 0.9823762290518943 1105 60-61 1.1376355561137288 1105 62-63 1.0598631506727898 1105 64-65 0.9401707274480771 1105 66-67 0.9509099551467628 1105 68-69 0.7892520511611778 1105 70-71 0.8723913154735712 1105 72-73 0.7374769908864849 1105 74-75 0.7008538198337071 1105 76-77 0.6394789835612187 1105 78-79 0.9179647509162159 1105 80-81 0.8910462688139624 1105 82-83 0.8447910861670955 1105 84-85 0.7873949457382245 1105 86-87 0.9630281699160967 1105 88-89 0.8471106895724958 1105 90-91 0.6499711865549678 1105 92-93 0.7820053717556057 1105 94-95 1.157359486380109 1105 96-97 1.3733823152778406 1105 98-99 1.2147118265788137 1105 100-101 1.1046500300175595 1106 1 0.1392266859296356 1106 2 -0.007365972100558338 1106 3 -0.04284200541565042 1106 4 0.13662051632999805 1106 5 0.12411153708102773 1106 6 0.1343780814295812 1106 7 0.040585582089121885 1106 8 0.053933513895820795 1106 9 0.30484106794828136 1106 10-11 -0.41876116833653754 1106 12-13 -0.22671781980329087 1106 14-15 -0.16434706452297831 1106 16-17 0.010912014125857183 1106 18-19 -0.02731411534419692 1106 20-21 0.0326053758353666 1106 22-23 -0.13309909484049598 1106 24-25 -0.030757667393039867 1106 26-27 -0.1155945385583621 1106 28-29 -0.09414530100006147 1106 30-31 0.07960708612117884 1106 32-33 0.0520125697445053 1106 34-35 -0.016394869182875027 1106 36-37 -0.17993669452959438 1106 38-39 0.017658287301408393 1106 40-41 0.009932390376661715 1106 42-43 -0.010928671118051625 1106 44-45 0.1392810845458996 1106 46-47 0.34606338246811674 1106 48-49 0.026921010413964552 1106 50-51 -0.03528073214548044 1106 52-53 0.021080338013987898 1106 54-55 -0.31045750124420124 1106 56-57 -0.21622792051301332 1106 58-59 -0.004250009023820667 1106 60-61 -0.3859234270253893 1106 62-63 -0.45848910487740824 1106 64-65 -0.30290817116216573 1106 66-67 -0.43155778529210664 1106 68-69 -0.6851894865221198 1106 70-71 -0.5129873433787608 1106 72-73 -0.38888455565678015 1106 74-75 -0.2788101599080939 1106 76-77 -0.22112693645362924 1106 78-79 -0.04542502480088828 1106 80-81 -0.22781648250530395 1106 82-83 -0.0032312852511289236 1106 84-85 -0.23214337811154806 1106 86-87 -0.34081933088459593 1106 88-89 -0.34261085002647107 1106 90-91 -0.35940286870608773 1106 92-93 -0.23146641852656913 1106 94-95 -0.3648338345213311 1106 96-97 -0.22365079286464962 1106 98-99 -0.22876247801849203 1106 100-101 -0.7892032177891473 1107 1 0.15391540721044805 1107 2 0.20326270797880852 1107 3 0.09361846212382119 1107 4 -0.07037109031559652 1107 5 0.06245401458804167 1107 6 0.02375347374903214 1107 7 0.020699637341742516 1107 8 0.06622232842052256 1107 9 0.13563851385208636 1107 10-11 0.06542669001699863 1107 12-13 0.010588583271520235 1107 14-15 -0.01824343741990475 1107 16-17 0.08421589512193606 1107 18-19 0.16358940952762424 1107 20-21 0.12527842821063473 1107 22-23 0.27899677223641106 1107 24-25 0.2400400031948351 1107 26-27 0.1756872981215949 1107 28-29 0.28872899809094577 1107 30-31 0.3147424793393725 1107 32-33 0.34505396357214835 1107 34-35 0.38229367796778035 1107 36-37 0.3815516127708136 1107 38-39 0.43555872698005516 1107 40-41 0.4340334083359352 1107 42-43 0.4624869902044111 1107 44-45 0.15809907780818833 1107 46-47 0.34617502236694975 1107 48-49 0.2838149497015223 1107 50-51 0.3464799765080926 1107 52-53 0.4123163953140434 1107 54-55 0.5853361320517294 1107 56-57 0.5501504952115042 1107 58-59 0.5154330179200173 1107 60-61 0.3947793004988256 1107 62-63 0.32646015162894315 1107 64-65 0.4960669886092788 1107 66-67 0.7035047260298697 1107 68-69 0.6650663022523844 1107 70-71 0.7086831546194432 1107 72-73 0.7312540182453304 1107 74-75 0.5458709642424928 1107 76-77 0.7651841863909823 1107 78-79 0.513401478370664 1107 80-81 0.42136601853736977 1107 82-83 0.31634248906214424 1107 84-85 0.3839452653724038 1107 86-87 0.672769140575614 1107 88-89 0.4237521186680162 1107 90-91 0.4903382531134035 1107 92-93 0.5100050655538659 1107 94-95 0.7193826710515339 1107 96-97 0.8384912046610182 1107 98-99 0.6798669350926652 1107 100-101 0.905063546802328 1108 1 0.03300721179701327 1108 2 0.07212008265690883 1108 3 0.03930122328970498 1108 4 0.11815245443612099 1108 5 0.004105688486511383 1108 6 0.051675299691815724 1108 7 -0.0703071440026406 1108 8 -0.08766995702007563 1108 9 -0.05118087552842354 1108 10-11 0.058554439256504054 1108 12-13 -0.06983981642347459 1108 14-15 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-0.7643786836253099 2316 88-89 -0.9071326753049895 2316 90-91 -0.6712260603291433 2316 92-93 -0.8349501613657573 2316 94-95 -1.0341379803381336 2316 96-97 -0.9158328391249739 2316 98-99 -1.0152224118798934 2316 100-101 -1.1582888126925326 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4691.0 3 662.0 4 578.0 5 529.0 6 505.0 7 582.0 8 696.0 9 771.0 10 1057.0 11 1562.0 12 2604.0 13 3923.0 14 4660.0 15 6346.0 16 8691.0 17 12869.0 18 10371.0 19 5769.0 20 5373.0 21 5127.0 22 5186.0 23 5579.0 24 6269.0 25 6759.0 26 7694.0 27 8786.0 28 10276.0 29 11837.0 30 14352.0 31 17700.0 32 21897.0 33 29198.0 34 41793.0 35 75881.0 36 95863.0 37 174845.0 38 195681.0 39 28221.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.26300682729812 24.366101074821074 15.895220891315622 19.47567120656518 2 13.972962126611998 24.28089679007613 41.718348497463325 20.02779258584854 3 15.773035438952537 32.10974442406461 32.55756838060963 19.559651756373224 4 9.98732354202761 21.024947940255302 42.79011475908739 26.197613758629696 5 9.211809791322647 42.097606453731245 36.69301397014239 11.997569784803726 6 23.22779385941255 44.35666468892524 19.10510197354621 13.31043947811599 7 22.831336922359842 37.82281168634321 24.82443285912332 14.52141853217363 8 23.391475189879486 36.678366916644705 22.162230155497518 17.767927737978294 9 26.133672279971197 17.824048838983973 24.17426681026376 31.868012070781067 10-11 21.374354967907912 30.42921856829686 29.731538437112633 18.464888026682598 12-13 22.967182205345253 30.382500692219455 29.497238913401247 17.153078189034048 14-15 21.065034173340603 31.109839885469892 28.753047447332325 19.072078493857184 16-17 16.60148141935909 34.26978844996091 30.313446291809065 18.815283838870933 18-19 16.350247430597527 32.079597114338036 34.87378673345486 16.696368721609577 20-21 17.265125430398427 31.458582595457536 35.10403554505351 16.17225642909053 22-23 17.734609569132633 31.001315534085272 34.59507189908967 16.669002997692424 24-25 17.090294850968647 32.298006383933625 34.59637828312093 16.0153204819768 26-27 16.28422738238517 32.17414381745955 35.27932755066524 16.262301249490037 28-29 16.208886500592183 31.216156228152624 36.03652176934445 16.538435501910737 30-31 16.766626706614737 31.607382604616554 35.2667528174233 16.359237871345407 32-33 16.254312805805565 32.44769665166308 35.02558477123482 16.272405771296526 34-35 16.76824647076832 31.767009300048947 35.07609648373914 16.38864774544359 36-37 17.3039710810691 31.109237978502506 34.36231816335554 17.224472777072858 38-39 16.22672314042657 32.46248990827007 34.632780813615035 16.678006137688328 40-41 15.969046570895513 31.671563034370404 35.948682472800456 16.410707921933632 42-43 16.181521873890105 31.59239811719779 34.80752426123252 17.418555747679587 44-45 16.161467973410826 31.653535951731826 34.700177185310444 17.484818889546904 46-47 16.956806580576856 31.503206210730806 34.1379401203916 17.402047088300744 48-49 16.566649799237673 31.747455663114525 33.95629040323136 17.72960413441644 50-51 16.367818404693423 31.529644394222206 33.95246812554681 18.15006907553756 52-53 16.51250776982398 31.986823336658837 34.06464493551196 17.436023958005222 54-55 17.017777926818752 31.50318335552979 33.67404499334106 17.804993724310393 56-57 16.963385335450784 32.08226442753357 33.000146113733976 17.954204123281666 58-59 16.458131609722972 30.98480643216129 34.073221104645974 18.483840853469765 60-61 17.188198975360137 32.043201355806346 33.05346871434945 17.71513095448407 62-63 16.80386055025655 31.656068995539577 33.949034173821765 17.591036280382102 64-65 17.024963727175667 30.94983601629478 33.76208021279258 18.263120043736972 66-67 16.888471611641556 31.696451559979543 32.73454181401437 18.68053501436453 68-69 16.69008935751569 31.070463877061112 33.571971332303015 18.667475433120185 70-71 17.166637818529846 31.015779831019792 33.709976204779046 18.107606145671312 72-73 17.319256814421006 30.520365256439508 33.211996423375986 18.948381505763503 74-75 16.807360811011513 31.412502934455055 32.775347702980454 19.004788551552988 76-77 16.90019227763669 30.7919670942364 33.56367527534744 18.74416535277946 78-79 16.778894803336307 30.390153941955916 33.353192304698496 19.47775895000928 80-81 17.097815484712346 31.064510353739028 32.4143315890982 19.423342572450427 82-83 17.447315836716072 30.566784770529804 32.63409139765952 19.351807995094607 84-85 16.849385199888626 30.22235794629518 32.708323977354034 20.219932876462153 86-87 16.95385816531932 30.617328005422113 33.04302843135963 19.385785397898932 88-89 17.640932146897764 29.74232339572761 33.235209034702464 19.381535422672165 90-91 17.25135357410051 29.645474099848514 33.50077180229163 19.60240052375935 92-93 17.83733056889564 29.933182774486923 32.9569362173078 19.272550439309633 94-95 17.397744888741933 28.837846191444854 33.65631356876388 20.108095351049325 96-97 17.96972842307137 28.675464282569855 33.10533720567387 20.249470088684905 98-99 17.50383881067537 28.960323106885426 32.991553418757356 20.544284663681857 100-101 17.844228211343403 28.350290427467495 33.835129789834376 19.970351571354726 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7844.0 1 4544.0 2 1793.0 3 2170.0 4 1540.0 5 1597.0 6 3352.5 7 6246.5 8 8680.0 9 8648.0 10 6125.0 11 4015.0 12 3527.5 13 3248.5 14 2886.0 15 2581.5 16 2353.0 17 2320.5 18 2434.0 19 2588.0 20 2821.0 21 3089.0 22 3395.0 23 3764.0 24 3965.5 25 4823.5 26 7330.0 27 9672.5 28 9779.0 29 10868.0 30 14981.0 31 18997.0 32 24012.0 33 30855.0 34 37222.0 35 41805.0 36 48673.5 37 54942.5 38 52918.0 39 48528.0 40 43635.0 41 37917.0 42 32961.5 43 31481.5 44 31061.0 45 28385.5 46 23485.5 47 18776.5 48 19526.5 49 17291.0 50 11303.0 51 7186.5 52 4514.0 53 3289.0 54 2599.0 55 2138.0 56 1819.0 57 1644.0 58 1466.0 59 1225.0 60 1069.0 61 968.0 62 824.5 63 615.5 64 449.0 65 381.0 66 330.0 67 290.0 68 248.0 69 220.5 70 199.5 71 177.5 72 157.0 73 119.0 74 85.0 75 68.0 76 60.0 77 50.5 78 43.0 79 31.0 80 24.0 81 17.0 82 10.5 83 8.0 84 7.0 85 4.5 86 1.5 87 1.0 88 0.5 89 1.5 90 2.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5093494479652962 2 0.05124625381503215 3 0.05747243418508279 4 0.06764984440535787 5 0.08249688990317093 6 0.10752134562125905 7 0.07208001120712466 8 0.053042267383315994 9 0.052683064669659226 10-11 0.07561217122474954 12-13 0.10895815647588612 14-15 0.056035623330455725 16-17 0.06112432844059326 18-19 0.060405923013279725 20-21 0.0762108424141775 22-23 0.06477622269610372 24-25 0.08914214010582111 26-27 0.06735050881064389 28-29 0.06675183762121595 30-31 0.0717806756124107 32-33 0.07237934680183865 34-35 0.07393589189435129 36-37 0.06872745254632817 38-39 0.0408293751189859 40-41 0.04555887751546667 42-43 0.04352339547141166 44-45 0.02173176417623443 46-47 0.028855951330426982 48-49 0.02879608421148419 50-51 0.02723953911897153 52-53 0.02658100081060079 54-55 0.02574286114540167 56-57 0.025982329621172844 58-59 0.02292910655509032 60-61 0.030592097779768027 62-63 0.02065415603526413 64-65 0.02292910655509032 66-67 0.016762793303982483 68-69 0.019935750607950595 70-71 0.042086584616784585 72-73 0.038015620528674555 74-75 0.03292691541853701 76-77 0.02304884079297591 78-79 0.015325982449355409 80-81 0.017541065850238808 82-83 0.02197123265200561 84-85 0.018618673991209114 86-87 0.010057675982389488 88-89 0.04280499004409812 90-91 0.05370080569168673 92-93 0.035141998819420414 94-95 0.05471854671371424 96-97 0.04472073785026755 98-99 0.03460319474893526 100-101 0.16906474389445186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 835183.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.18200415242077 #Duplication Level Percentage of deduplicated Percentage of total 1 75.69901436036959 14.520588077947691 2 9.164142440719013 3.5157323670249507 3 3.5811617299189247 2.0608157752138547 4 1.940648529110832 1.4890211257517294 5 1.2066166173274855 1.1572662481977865 6 0.8894081842575883 1.0236378890175644 7 0.630070140720888 0.8460205638937087 8 0.5262975525951074 0.8076353471430592 9 0.4404937510812482 0.7604597666120314 >10 4.154972139184428 17.42310540731835 >50 0.8826482898151158 11.897784069370026 >100 0.821927095409395 30.177820783678555 >500 0.05070532728725133 6.652787822245538 >1k 0.00938987542356506 2.7854436342616014 >5k 0.0012519833898086746 1.9079154159926968 >10k+ 0.0012519833898086746 2.973965706330865 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14335 1.7163903000899206 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10432 1.249067569622466 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8024 0.9607475247939673 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7865 0.9417097809701587 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3031 0.3629144750312207 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2502 0.29957506318974403 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2380 0.2849674861677022 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1802 0.21576109666983165 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1690 0.20235086202664565 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1451 0.17373437917198986 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1322 0.15828866248474885 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1279 0.15314009025566852 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1180 0.1412864007049952 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1168 0.13984958985036813 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1142 0.1367364996653428 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1103 0.13206686438780482 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1063 0.12727749487238127 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1051 0.1258406840177542 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 1033 0.12368546773581358 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 963 0.11530407108382235 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 957 0.11458566565650881 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 956 0.11446593141862323 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 953 0.11410672870496646 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 948 0.1135080575155385 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 939 0.11243044937456821 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 929 0.11123310699571233 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 910 0.10895815647588612 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 905 0.10835948528645817 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 902 0.1080002825728014 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 895 0.10716214290760227 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 880 0.10536612933931845 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 858 0.10273197610583548 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 853 0.10213330491640756 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 838 0.1003372913481237 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.1973423788558915E-4 0.0 16-17 0.0 0.0 0.0 1.7960135682838373E-4 0.0 18-19 0.0 0.0 0.0 2.9933559471397286E-4 0.0 20-21 0.0 0.0 0.0 5.986711894279457E-4 0.0 22-23 0.0 0.0 0.0 0.001376943735684275 0.0 24-25 0.0 0.0 0.0 0.00562750918062269 0.0 26-27 0.0 0.0 0.0 0.013350367524243189 0.0 28-29 0.0 0.0 0.0 0.02963422387668331 0.0 30-31 0.0 0.0 0.0 0.07202014408818187 0.0 32-33 0.0 0.0 0.0 0.14463895936579169 0.0 34-35 0.0 0.0 0.0 0.2543155212689914 0.0 36-37 0.0 0.0 0.0 0.4161962108903079 0.0 38-39 0.0 0.0 0.0 0.6563830920887996 0.0 40-41 0.0 0.0 0.0 0.907525656053823 0.0 42-43 0.0 0.0 0.0 1.1518433684593676 0.0 44-45 0.0 1.1973423788558915E-4 0.0 1.421005935226172 0.0 46-47 0.0 1.1973423788558915E-4 0.0 1.742193028354265 0.0 48-49 0.0 1.1973423788558915E-4 0.0 2.066134008953726 0.0 50-51 0.0 1.1973423788558915E-4 0.0 2.386363228178735 0.0 52-53 0.0 1.1973423788558915E-4 0.0 2.6929427442847853 0.0 54-55 0.0 1.1973423788558915E-4 0.0 3.0182008015009885 0.0 56-57 0.0 1.1973423788558915E-4 0.0 3.3835698284088638 0.0 58-59 0.0 1.1973423788558915E-4 0.0 3.7759389259599394 0.0 60-61 0.0 1.1973423788558915E-4 0.0 4.176210483211464 0.0 62-63 0.0 1.1973423788558915E-4 0.0 4.54888329863036 0.0 64-65 0.0 1.1973423788558915E-4 0.0 4.929937510701247 0.0 66-67 0.0 1.1973423788558915E-4 0.0 5.36187877387351 0.0 68-69 0.0 1.1973423788558915E-4 0.0 5.811660438490726 0.0 70-71 0.0 1.1973423788558915E-4 0.0 6.313706097945001 0.0 72-73 0.0 1.1973423788558915E-4 0.0 6.781388031126113 0.0 74-75 0.0 1.1973423788558915E-4 0.0 7.245897007003256 0.0 76-77 0.0 1.1973423788558915E-4 0.0 7.729563460942092 0.0 78-79 0.0 1.1973423788558915E-4 0.0 8.257052645947056 0.0 80-81 0.0 1.1973423788558915E-4 0.0 8.829681638634886 0.0 82-83 0.0 1.1973423788558915E-4 0.0 9.387283984468075 0.0 84-85 0.0 1.7960135682838373E-4 0.0 9.926267656310053 0.0 86-87 0.0 2.394684757711783E-4 0.0 10.50566163343842 0.0 88-89 0.0 2.394684757711783E-4 0.0 11.097448104187944 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 20 0.0020707604 71.277016 2 GTATGGT 55 7.919898E-7 52.06882 1 GGGCCTA 75 1.3517638E-7 44.376747 7 ATCACGC 45 9.605613E-4 42.238235 3 CACGCAG 50 0.0016125551 38.014412 5 GGGATGC 205 0.0 37.109474 7 GGATGCC 225 0.0 35.924038 8 GGTATCA 5705 0.0 35.222134 1 GTATCAA 8845 0.0 32.755184 1 CGCAGAA 60 0.003931452 31.697678 9 GGTACTC 150 9.513315E-10 31.678677 3 TACAGCA 80 4.4826316E-4 29.698757 4 CATATAG 225 0.0 29.566765 3 CATGGGT 455 0.0 29.241856 4 TCACGCA 65 0.00583235 29.241854 4 GCCTATG 115 3.8335675E-6 28.941359 9 GTGGTAC 170 3.4560799E-9 28.076324 1 CATTCCG 205 3.092282E-11 27.832108 9 GACGATC 35 0.008356326 27.166996 94-95 GGCCTAT 125 7.331757E-6 26.62605 8 >>END_MODULE