##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938213_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 835183 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51931732326927 34.0 31.0 34.0 31.0 34.0 2 32.721538872319 34.0 31.0 34.0 31.0 34.0 3 32.759598794515696 34.0 31.0 34.0 31.0 34.0 4 36.23167257954245 37.0 35.0 37.0 35.0 37.0 5 36.21937707065398 37.0 37.0 37.0 35.0 37.0 6 36.27474577427941 37.0 37.0 37.0 35.0 37.0 7 36.23998213565171 37.0 37.0 37.0 35.0 37.0 8 36.24663935927815 37.0 37.0 37.0 35.0 37.0 9 37.927983447938956 39.0 38.0 39.0 35.0 39.0 10-11 37.93383306413085 39.0 38.0 39.0 35.0 39.0 12-13 37.90217772631866 39.0 38.0 39.0 35.0 39.0 14-15 39.15660160707294 40.5 38.5 41.0 36.0 41.0 16-17 39.13299480473142 40.0 38.5 41.0 36.0 41.0 18-19 39.2430347600466 40.5 39.0 41.0 36.0 41.0 20-21 39.22118086694772 40.5 39.0 41.0 36.0 41.0 22-23 39.06007126581839 40.0 38.5 41.0 35.0 41.0 24-25 38.996889903170924 40.0 38.5 41.0 35.0 41.0 26-27 38.88010471956446 40.0 38.0 41.0 35.0 41.0 28-29 38.79723186415433 40.0 38.0 41.0 35.0 41.0 30-31 38.52261360683826 40.0 37.5 41.0 35.0 41.0 32-33 38.24710452679233 40.0 37.0 41.0 34.5 41.0 34-35 38.022821944412186 40.0 36.5 41.0 34.0 41.0 36-37 37.837965451883 40.0 36.0 41.0 34.0 41.0 38-39 37.72766148257328 40.0 36.0 41.0 33.0 41.0 40-41 37.647357525236984 40.0 36.0 41.0 33.0 41.0 42-43 37.44975831644083 40.0 36.0 41.0 33.0 41.0 44-45 37.16330073768264 40.0 36.0 41.0 33.0 41.0 46-47 36.728369111919186 40.0 36.0 41.0 31.5 41.0 48-49 36.337957070486354 40.0 35.0 41.0 30.5 41.0 50-51 36.070584530575935 40.0 35.0 41.0 30.0 41.0 52-53 35.75173524844256 40.0 35.0 41.0 28.0 41.0 54-55 35.57571214931339 39.5 35.0 41.0 27.5 41.0 56-57 35.38655480295935 39.0 35.0 41.0 27.0 41.0 58-59 35.12920342008877 39.0 35.0 41.0 26.0 41.0 60-61 34.84603973021481 39.0 35.0 40.5 25.5 41.0 62-63 34.524061792445494 38.5 35.0 40.0 23.5 41.0 64-65 34.164101759734095 38.0 35.0 40.0 22.0 41.0 66-67 33.75322055166353 37.5 34.0 40.0 20.5 41.0 68-69 33.28656773425705 37.0 34.0 39.5 16.5 41.0 70-71 32.81158500592086 36.5 33.5 39.0 10.5 40.5 72-73 32.39942862821681 36.0 33.0 39.0 7.5 40.0 74-75 31.917530648971542 35.0 33.0 38.0 4.0 40.0 76-77 30.52200894893694 34.5 30.5 36.0 2.0 39.0 78-79 31.169311995095686 35.0 32.0 37.0 2.0 39.0 80-81 30.938922367912184 35.0 32.0 36.0 2.0 39.0 82-83 30.558165096751253 35.0 32.0 36.0 2.0 37.5 84-85 30.15209121833179 35.0 31.0 35.5 2.0 37.0 86-87 29.76817595664663 35.0 31.0 35.0 2.0 36.5 88-89 29.34338701817446 34.0 31.0 35.0 2.0 36.0 90-91 29.042423037825245 34.0 30.0 35.0 2.0 36.0 92-93 28.714938522455558 34.0 30.0 35.0 2.0 35.5 94-95 28.39956452657681 34.0 29.0 35.0 2.0 35.0 96-97 27.945271275876067 34.0 29.0 35.0 2.0 35.0 98-99 27.44975232972893 34.0 29.0 35.0 2.0 35.0 100-101 25.275301341143198 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11341025361317492 1101 2 0.09745268599244383 1101 3 0.08276098177999813 1101 4 0.07178999078765003 1101 5 0.003879571628182532 1101 6 0.031568801563629734 1101 7 0.08703879796199487 1101 8 0.08513909120564733 1101 9 0.08961081563690954 1101 10-11 0.1993743946257922 1101 12-13 0.11874027888772787 1101 14-15 0.22756851773601028 1101 16-17 0.1968743001229285 1101 18-19 0.26429393577831917 1101 20-21 0.2360544770050339 1101 22-23 0.26222123441579726 1101 24-25 0.23711260029764247 1101 26-27 0.27703167214031765 1101 28-29 0.26645478872882933 1101 30-31 0.2397270889745613 1101 32-33 0.2751521543687616 1101 34-35 0.17778271111449584 1101 36-37 0.3259533642307417 1101 38-39 0.5601886299008569 1101 40-41 0.46104175239063494 1101 42-43 0.4590037722532756 1101 44-45 0.507728115569364 1101 46-47 0.6906091009500628 1101 48-49 0.729398065117131 1101 50-51 0.3679018850532074 1101 52-53 0.5590043003194509 1101 54-55 0.35973261625878195 1101 56-57 0.42150735583155097 1101 58-59 0.35884284297318914 1101 60-61 0.49672112480271124 1101 62-63 0.5280011091243395 1101 64-65 0.4103054211775543 1101 66-67 0.4333232604707007 1101 68-69 0.6061628557631096 1101 70-71 0.7817953257030794 1101 72-73 0.5844653535125914 1101 74-75 0.6242836353667833 1101 76-77 0.5216892433359561 1101 78-79 0.6887081400010615 1101 80-81 0.7459726803696043 1101 82-83 0.6250507922949957 1101 84-85 0.6568764242796945 1101 86-87 0.5676423629127605 1101 88-89 0.4722105803265926 1101 90-91 0.4756821270965155 1101 92-93 0.5760343552277831 1101 94-95 0.45702453642524077 1101 96-97 0.5844493529163941 1101 98-99 0.6398459209720535 1101 100-101 1.2164384933107115 1102 1 0.10778025228265165 1102 2 0.1582688651184796 1102 3 0.08172887822750852 1102 4 0.12882559170761 1102 5 -0.004611048204175461 1102 6 0.03990356045435561 1102 7 -0.01781570014676248 1102 8 0.1047357535005986 1102 9 -0.017243264308348216 1102 10-11 -0.08692789957123637 1102 12-13 0.10418153641943206 1102 14-15 0.03486547084467162 1102 16-17 0.08612084552217425 1102 18-19 0.10880078795752013 1102 20-21 0.14918956282358664 1102 22-23 0.15554582433045994 1102 24-25 0.1959063886688952 1102 26-27 0.006508397539938926 1102 28-29 0.06022510292168448 1102 30-31 0.10064495786129868 1102 32-33 -0.08946170965207045 1102 34-35 0.09703628926354924 1102 36-37 0.25671309128401987 1102 38-39 0.11082727961288441 1102 40-41 0.050588615214003596 1102 42-43 0.00672002930978266 1102 44-45 0.019957497257607315 1102 46-47 0.1366393987965253 1102 48-49 0.05106938860461696 1102 50-51 0.030440137509920362 1102 52-53 0.08581998525226453 1102 54-55 0.07898334329306067 1102 56-57 -0.020019131024810122 1102 58-59 -0.14015831648016075 1102 60-61 -0.4521422307693186 1102 62-63 -0.3787721892353275 1102 64-65 -0.4159999142085766 1102 66-67 -0.44709813429465584 1102 68-69 -0.10190674945253164 1102 70-71 -0.08722921289151486 1102 72-73 -0.041273560402743215 1102 74-75 -0.03725862765806198 1102 76-77 -0.12321961602805942 1102 78-79 -0.08425663922761473 1102 80-81 0.03376515722805351 1102 82-83 0.2046063985370381 1102 84-85 0.08754871714620549 1102 86-87 0.24345160432615742 1102 88-89 -0.0028887335051166474 1102 90-91 0.1563306606731416 1102 92-93 -0.004177957786609454 1102 94-95 -0.34877653179243495 1102 96-97 -0.27339881571754177 1102 98-99 -0.2732207380916414 1102 100-101 -0.18147163955123702 1103 1 0.06796918615579983 1103 2 0.1529382308404763 1103 3 0.07595964745827644 1103 4 0.047179167955924584 1103 5 0.11208935665412412 1103 6 0.060720024691875096 1103 7 0.08670521753204952 1103 8 0.10237408008629956 1103 9 0.05026685714913981 1103 10-11 0.00397965778368814 1103 12-13 -0.010309016886914435 1103 14-15 0.13397140876640634 1103 16-17 0.18456888870706223 1103 18-19 0.206186078105965 1103 20-21 0.1577928016899861 1103 22-23 0.25983057466783777 1103 24-25 0.126641881246492 1103 26-27 0.2154490183226656 1103 28-29 0.24139919199051008 1103 30-31 0.2474735678477984 1103 32-33 0.24501872219678233 1103 34-35 0.236965439061116 1103 36-37 0.46297152583192513 1103 38-39 0.3857260650379928 1103 40-41 0.294094013324667 1103 42-43 0.2883495839656476 1103 44-45 0.1806525040052378 1103 46-47 0.41131888597601574 1103 48-49 0.6106341659325523 1103 50-51 0.5295123372400141 1103 52-53 0.6460595264128628 1103 54-55 0.6152181611068173 1103 56-57 0.43782499852984813 1103 58-59 0.6843524797411575 1103 60-61 0.6482963657219116 1103 62-63 0.7309106717633327 1103 64-65 0.6429407065741444 1103 66-67 0.5356953879725523 1103 68-69 0.6622970292249235 1103 70-71 0.8419880610626009 1103 72-73 0.47594978642586483 1103 74-75 0.4252751105335726 1103 76-77 0.39731007358057724 1103 78-79 0.6045254120544357 1103 80-81 0.500735467619414 1103 82-83 0.7598358182401448 1103 84-85 0.6252511462960015 1103 86-87 0.858144586782295 1103 88-89 0.705596421690565 1103 90-91 0.3711417267999977 1103 92-93 0.31532271697722436 1103 94-95 0.4831735356839495 1103 96-97 0.534310361070581 1103 98-99 0.7680242011796103 1103 100-101 0.7718684414204233 1104 1 0.19357337519983275 1104 2 0.1179758248254359 1104 3 0.10458478172250807 1104 4 0.07530752456172252 1104 5 0.12581116116540159 1104 6 0.10369800535782048 1104 7 0.16288674277372195 1104 8 0.13996806504870563 1104 9 0.13959285070446015 1104 10-11 0.1775631701360041 1104 12-13 0.20027771780696213 1104 14-15 0.22560674711227335 1104 16-17 0.3340922506834261 1104 18-19 0.2538096011994142 1104 20-21 0.18951272650201645 1104 22-23 0.1966995327774015 1104 24-25 0.17374848704671564 1104 26-27 0.3139855381937693 1104 28-29 0.360558697898135 1104 30-31 0.28420826172535385 1104 32-33 0.2702872506608003 1104 34-35 0.3852938493726086 1104 36-37 0.4613335022014198 1104 38-39 0.4583831541894412 1104 40-41 0.18272183287353982 1104 42-43 0.19641215109776766 1104 44-45 0.31554106469267396 1104 46-47 0.442897833344432 1104 48-49 0.12441638934823374 1104 50-51 0.08274810523142406 1104 52-53 0.14359577775550036 1104 54-55 0.06214351019382036 1104 56-57 0.06106613709053477 1104 58-59 0.25689544429540234 1104 60-61 0.10178723575413784 1104 62-63 0.09192033878803585 1104 64-65 0.08125971838939705 1104 66-67 0.05466021074376215 1104 68-69 -0.008480360678397858 1104 70-71 -0.16659142336275323 1104 72-73 0.01148281542693752 1104 74-75 0.1120836497387252 1104 76-77 -0.07395066755798041 1104 78-79 -0.13283361050195452 1104 80-81 -0.057092137106685925 1104 82-83 0.08725257441629708 1104 84-85 0.16905485198880754 1104 86-87 -0.012948324066254457 1104 88-89 0.03462434865082287 1104 90-91 -0.11501563087991684 1104 92-93 0.2053603109621882 1104 94-95 0.2736474884229416 1104 96-97 0.32417341155393586 1104 98-99 0.23392108624942765 1104 100-101 0.18132064335382836 1105 1 0.08801620195833237 1105 2 0.007731230709879355 1105 3 0.08910774723626247 1105 4 0.014529305085346778 1105 5 -0.025376360989859847 1105 6 -0.12280158951734421 1105 7 0.039735124806441036 1105 8 0.08085782045458956 1105 9 -0.11467045511414398 1105 10-11 -0.05112188328983791 1105 12-13 -0.014620431278977719 1105 14-15 -0.021956173837750725 1105 16-17 0.07246231955465277 1105 18-19 0.10519841975413868 1105 20-21 0.13054649112449113 1105 22-23 0.17325293329393077 1105 24-25 0.1380072985117451 1105 26-27 0.12640957728832092 1105 28-29 0.08441865130878057 1105 30-31 0.2750169921654191 1105 32-33 0.24208676451100786 1105 34-35 0.3134395803697245 1105 36-37 0.6256021252834145 1105 38-39 0.4815338226454102 1105 40-41 0.38065696226028223 1105 42-43 0.3967226412988154 1105 44-45 0.43962795130836696 1105 46-47 0.2194176844610567 1105 48-49 0.34952178510454956 1105 50-51 0.37653956722564885 1105 52-53 0.46487400989202854 1105 54-55 0.5626910617206349 1105 56-57 0.3309995446967591 1105 58-59 0.19909552532036656 1105 60-61 0.38716602746933404 1105 62-63 0.32713961260177626 1105 64-65 0.4077017095779283 1105 66-67 0.522406893784229 1105 68-69 0.5743154437475866 1105 70-71 0.6637720495698503 1105 72-73 0.4529691804444056 1105 74-75 0.3869719239069589 1105 76-77 0.40715563972920066 1105 78-79 0.30602893823962773 1105 80-81 0.4489625800655439 1105 82-83 0.6184978268597057 1105 84-85 0.5003409334188973 1105 86-87 0.5399014759033598 1105 88-89 0.4272187933766034 1105 90-91 0.4569161815472924 1105 92-93 0.5582050468714428 1105 94-95 0.48238014401619367 1105 96-97 0.5822126073811518 1105 98-99 0.4895453582907514 1105 100-101 0.45076030507459564 1106 1 -0.06805392348803707 1106 2 -0.0901923640423874 1106 3 -0.00860482463873069 1106 4 0.08460536311813627 1106 5 0.10280581910095776 1106 6 0.01623554751581935 1106 7 0.1364195213857755 1106 8 -0.07436073040192781 1106 9 0.04072619306432301 1106 10-11 -0.13156649801594256 1106 12-13 -0.06937113992672295 1106 14-15 -0.18691694752843802 1106 16-17 -0.1704212706478998 1106 18-19 -0.14254019324187794 1106 20-21 -0.0384566427897326 1106 22-23 0.0029881519645158505 1106 24-25 -0.04677091794085442 1106 26-27 -0.1438980072630116 1106 28-29 -0.027044081452451962 1106 30-31 0.027690696918973856 1106 32-33 -0.10726684697342392 1106 34-35 -0.08908131987482193 1106 36-37 0.05358720102714898 1106 38-39 0.022434845432890427 1106 40-41 0.09253141412461474 1106 42-43 -0.12658042047851126 1106 44-45 0.06105216116986867 1106 46-47 -0.017822914544595392 1106 48-49 0.172534204025375 1106 50-51 0.3818113976171347 1106 52-53 0.3775995873613667 1106 54-55 -0.009938971773635785 1106 56-57 -0.09794336538354997 1106 58-59 -0.06947822942016302 1106 60-61 -0.12125318668746132 1106 62-63 -0.056374262526560415 1106 64-65 -0.36327635900899935 1106 66-67 -0.34315357399812285 1106 68-69 -0.4672201442878645 1106 70-71 -0.4857720811505928 1106 72-73 -0.42909671855794684 1106 74-75 -0.22437332448642522 1106 76-77 -0.1968958498528508 1106 78-79 -0.16915641767066347 1106 80-81 -0.3902095517444337 1106 82-83 -0.305155293435309 1106 84-85 -0.16306784932331908 1106 86-87 -0.0800410839687693 1106 88-89 -0.06498084324499587 1106 90-91 -0.17234281386408412 1106 92-93 -0.23275359563784548 1106 94-95 -0.2670788292452606 1106 96-97 -0.44468382977444065 1106 98-99 -0.363376186097387 1106 100-101 -0.2869008430694535 1107 1 0.11229857773719232 1107 2 0.16304484195499214 1107 3 0.04243075829707266 1107 4 0.07202445929836898 1107 5 -0.04506859935347052 1107 6 0.08835773071479025 1107 7 0.03649689696345604 1107 8 0.005550968757333408 1107 9 0.03295633696043154 1107 10-11 0.112238189736388 1107 12-13 0.08533999359038091 1107 14-15 0.16821027280659706 1107 16-17 0.16928489875546404 1107 18-19 0.07341771219702053 1107 20-21 0.200598083229103 1107 22-23 0.11368297341790878 1107 24-25 0.0326724553534703 1107 26-27 0.02472387893170236 1107 28-29 0.05395663332375733 1107 30-31 0.13664702535653106 1107 32-33 0.1480669422813392 1107 34-35 0.11160930279496029 1107 36-37 0.14503221451521853 1107 38-39 0.2261341282694147 1107 40-41 0.22702168319784022 1107 42-43 0.24402402180647442 1107 44-45 0.2824186335316199 1107 46-47 0.3252464945784226 1107 48-49 0.3950599057769253 1107 50-51 0.4411560784323427 1107 52-53 0.4249096275096065 1107 54-55 0.3579208141305301 1107 56-57 0.25971014649871904 1107 58-59 0.12852180147390158 1107 60-61 0.250990747193633 1107 62-63 0.25347209569568463 1107 64-65 0.3736143785305188 1107 66-67 0.46194071607745 1107 68-69 0.40635223417560695 1107 70-71 0.5761311895473753 1107 72-73 0.38431860573840737 1107 74-75 0.1281433477739391 1107 76-77 0.05076497830455651 1107 78-79 0.3551143771913772 1107 80-81 0.4713239732256902 1107 82-83 0.3229799270570055 1107 84-85 0.410363118601623 1107 86-87 0.45092459413478636 1107 88-89 0.4768581397225482 1107 90-91 0.42995092966746995 1107 92-93 0.5468495842034997 1107 94-95 0.7236627826663629 1107 96-97 0.8676684580009209 1107 98-99 0.8189823226182504 1107 100-101 0.7547478605736586 1108 1 0.059442624997657845 1108 2 0.13962084647045714 1108 3 0.07136205207120838 1108 4 0.08870781474772826 1108 5 0.07053310588734973 1108 6 0.11909629754183015 1108 7 0.07180520526361533 1108 8 0.005852180932819806 1108 9 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-1.042631210472969 2316 60-61 -0.9205899660771664 2316 62-63 -1.1546556680405544 2316 64-65 -1.1612618149387615 2316 66-67 -1.001076675265395 2316 68-69 -1.2102364908871408 2316 70-71 -1.3108986709766235 2316 72-73 -1.2196100494756763 2316 74-75 -1.2067866265086558 2316 76-77 -1.0692923878963043 2316 78-79 -1.0981887164226514 2316 80-81 -1.110416223395191 2316 82-83 -1.0852662429965996 2316 84-85 -0.9980103105781168 2316 86-87 -1.2763436061334552 2316 88-89 -1.2140128438687725 2316 90-91 -1.300081006840184 2316 92-93 -1.5305848275494895 2316 94-95 -1.495453640621836 2316 96-97 -1.5400017261817673 2316 98-99 -1.3045818624585728 2316 100-101 -1.1636878923798584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 5.0 5 25.0 6 47.0 7 128.0 8 249.0 9 448.0 10 846.0 11 1419.0 12 2338.0 13 3392.0 14 4549.0 15 6183.0 16 8075.0 17 11830.0 18 10993.0 19 6521.0 20 4835.0 21 4629.0 22 4952.0 23 5487.0 24 6098.0 25 6650.0 26 7258.0 27 7856.0 28 9014.0 29 11101.0 30 13248.0 31 16027.0 32 19889.0 33 25869.0 34 36166.0 35 67849.0 36 85533.0 37 176627.0 38 236784.0 39 32259.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.26109607116045 24.781155746704613 16.19297806588496 19.76477011624997 2 13.80156470984129 24.405449779232928 41.56863778272496 20.224347728200822 3 15.667348006691261 31.916050287444065 32.886690399811286 19.52991130605339 4 9.888371770019265 21.47684998377601 42.22056722897856 26.414211017226165 5 9.004014688996303 42.30031023141036 36.86162194393325 11.834053135660088 6 23.134570507301994 44.44079920209104 18.945189257923115 13.479441032683855 7 22.730587188676015 37.85194382548495 24.438835560589713 14.978633425249315 8 23.301260330133218 36.87202009631479 22.107622566686384 17.719097006865603 9 25.697813644962764 17.8068200866879 24.138269594578414 32.357096673770926 10-11 21.260909285749353 30.511516637671026 29.628057563432208 18.599516513147417 12-13 22.774110584147426 30.227028088454865 29.49162039936158 17.50724092803613 14-15 20.846449221308383 31.094083572103358 28.57301932630334 19.486447880284917 16-17 16.555453249056193 34.33531174679501 30.18004480465911 18.929190199489693 18-19 16.269967180845395 31.989097000298138 34.73131038347284 17.009625435383622 20-21 17.00058550042326 31.44484502198919 34.97035978941142 16.584209688176124 22-23 17.587043797586876 31.10815234505492 34.43724309522584 16.86756076213237 24-25 17.086291200846812 32.32874104257832 34.54330246194761 16.041665294627254 26-27 16.286914121094064 32.16286794359969 35.2562977884312 16.293920146875042 28-29 16.111209226725894 31.21605158154056 35.94567654374942 16.727062647984123 30-31 16.63809009522464 31.618339932685412 35.30166442564085 16.4419055464491 32-33 15.959802653202097 32.343765621641815 35.208359400583944 16.48807232457214 34-35 16.523554779517873 31.762755221188705 35.09564544658693 16.61804455270649 36-37 17.076374486917288 31.067128221584678 34.55728804954332 17.29920924195472 38-39 16.07124145004997 32.44451177487472 34.6325445094396 16.851702265635712 40-41 15.883538164453693 31.644926126072182 36.01922069368142 16.452315015792703 42-43 16.205976370650173 31.423814116899003 34.92175539497705 17.44845411747378 44-45 16.033933617510417 31.687760429139328 34.69975813017865 17.578547823171608 46-47 16.857513178257445 31.39265968815199 34.1327793625944 17.61704777099616 48-49 16.457111794660573 31.691437684914565 33.917357034326606 17.934093486098256 50-51 16.276013759858618 31.378392519962688 34.00721757985974 18.33837614031895 52-53 16.352045000915965 31.954553672668144 34.21459727987759 17.478804046538304 54-55 16.874505347929734 31.479567950976012 33.59778635340997 18.04814034768428 56-57 16.87695989980639 32.07027681358457 32.88123680678366 18.171526479825378 58-59 16.260867378766093 31.258538547839215 33.83474041018555 18.64585366320914 60-61 16.96610203991221 32.47312265695063 32.68541146072178 17.875363842415375 62-63 16.563256196546146 32.25825956706495 33.42483024678424 17.753653989604672 64-65 16.667664451982382 31.586909695240443 33.234812011259805 18.51061384151737 66-67 16.5931298888986 32.186838094166184 32.288252993655284 18.931779023279926 68-69 16.503538452420223 31.51919672550218 32.994145583923974 18.983119238153623 70-71 17.017408160846188 31.43197359141649 33.0611973663257 18.48942088141162 72-73 17.184736758291297 30.870719351327793 32.56112732179654 19.38341656858437 74-75 16.631325110784104 31.8315267432407 31.8747508031174 19.662397342857794 76-77 16.606360522184957 31.123298726147443 33.00097104466925 19.269369706998347 78-79 16.468366813021817 30.724344245512658 32.63775723404332 20.169531707422205 80-81 16.802964140793094 31.41802455270282 31.70784127550489 20.071170030999195 82-83 17.204373173304532 30.92861085534547 31.90408569139937 19.962930279950623 84-85 16.5486547116076 30.497254702866712 31.985660155503975 20.968430430021716 86-87 16.622863786412765 30.883784473066317 32.3038958022273 20.189455938293616 88-89 17.24917443144129 30.155942058138223 32.2899679351327 20.304915575287783 90-91 16.865585861730406 29.807526521228954 32.73042936851936 20.596458248521277 92-93 17.392119064153835 30.162958883115014 32.048181230393446 20.3967408223377 94-95 16.843793515912083 29.031781058761975 32.716901565285696 21.407523860040254 96-97 17.231623028499758 29.05724198004628 32.11842920559279 21.592705785861174 98-99 16.605701983876585 29.333870540947316 32.097576219822486 21.962851255353616 100-101 16.654074615982363 28.99981201724652 32.842921850660275 21.50319151611084 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7486.0 1 4324.0 2 1722.5 3 2142.0 4 1522.0 5 1550.0 6 3423.0 7 6495.5 8 8758.0 9 8511.0 10 5991.0 11 3893.0 12 3458.0 13 3166.5 14 2732.5 15 2412.0 16 2189.5 17 2142.0 18 2225.5 19 2377.0 20 2613.0 21 2886.0 22 3081.5 23 3312.5 24 3521.0 25 4332.5 26 6877.0 27 9328.0 28 9264.0 29 10221.5 30 14433.0 31 18216.5 32 23327.0 33 30472.5 34 36957.0 35 42153.0 36 49094.5 37 54937.5 38 53019.0 39 48564.5 40 43711.5 41 38154.5 42 32699.5 43 31465.5 44 31567.5 45 29027.5 46 24697.5 47 20386.0 48 21198.0 49 18558.0 50 12295.0 51 8397.0 52 5519.0 53 3692.5 54 2602.0 55 2126.5 56 1738.0 57 1402.0 58 1282.0 59 1071.5 60 891.0 61 791.0 62 621.5 63 435.5 64 287.5 65 203.0 66 153.5 67 134.5 68 131.0 69 108.0 70 82.5 71 73.0 72 64.5 73 55.0 74 42.5 75 33.0 76 28.0 77 25.5 78 17.5 79 9.0 80 6.5 81 3.5 82 2.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016762793303982483 3 0.0076629912246777045 4 0.0 5 0.0 6 0.0 7 0.0 8 5.986711894279457E-4 9 3.5920271365676745E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.3946847577117826E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006166313251107842 26-27 0.022450169603547964 28-29 5.9867118942794566E-5 30-31 0.0 32-33 4.1906983259956204E-4 34-35 0.020594288916321334 36-37 0.004190698325995621 38-39 0.019576547894293826 40-41 0.017421331612353222 42-43 0.00927940343613316 44-45 0.00275388747136855 46-47 5.9867118942794566E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.7960135682838373E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0032926915418537016 86-87 2.3946847577117826E-4 88-89 1.1973423788558913E-4 90-91 3.5920271365676745E-4 92-93 3.5920271365676745E-4 94-95 0.0 96-97 5.9867118942794566E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 835183.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.39261152665863 #Duplication Level Percentage of deduplicated Percentage of total 1 73.014771736488 12.6991756052039 2 10.163867369002707 3.5355239351509202 3 3.888367898063705 2.0288661697125656 4 2.130348418463984 1.4820928983510266 5 1.4237875408022993 1.2381691796835508 6 0.9544290297383194 0.9960008006402584 7 0.7382170509353261 0.8987665672491586 8 0.5879289006142498 0.8180495178943309 9 0.48848643273847653 0.7646449284597224 >10 4.659801169372422 17.848463658318874 >50 0.9505448061522686 11.689884387244808 >100 0.9229927827855363 30.70664203731018 >500 0.06130325199097964 7.175924746475401 >1k 0.01239841051502959 3.324581172297348 >5k 0.0013776011683366211 1.8973988903767576 >10k+ 0.0013776011683366211 2.895815505631203 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13792 1.6513746089180454 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10380 1.2428413892524153 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8151 0.9759537730054372 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7687 0.9203970866265238 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3279 0.39260856602684685 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2366 0.2832912068373039 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2270 0.2717967200002874 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 2026 0.24258156595620362 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1834 0.21959259228217048 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1705 0.20414687559492947 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1474 0.1764882666433584 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1327 0.1588873336741768 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1326 0.1587675994362912 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1285 0.15385849568298207 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1248 0.14942832888121524 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1215 0.14547709903099082 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 1199 0.1435613512248214 RNA PCR Primer, Index 27 (95% over 23bp) GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1079 0.12919324267855067 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1068 0.1278761660618092 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1028 0.12308679654638566 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1020 0.12212892264330093 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1002 0.11997370636136033 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 985 0.11793822431730532 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 983 0.11769875584153414 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 982 0.11757902160364855 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 952 0.11398699446708088 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 928 0.11111337275782672 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 919 0.11003576461685644 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 915 0.10955682766531406 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 902 0.1080002825728014 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 892 0.10680294019394551 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 882 0.10560559781508962 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 872 0.10440825543623372 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 870 0.10416878696046256 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 864 0.10345038153314902 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 857 0.1026122418679499 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.986711894279457E-5 0.0 18-19 0.0 0.0 0.0 1.7960135682838373E-4 0.0 20-21 0.0 0.0 0.0 4.789369515423566E-4 0.0 22-23 0.0 0.0 0.0 0.001257209497798686 0.0 24-25 0.0 0.0 0.0 0.0055077749427371 0.0 26-27 0.0 0.0 0.0 0.013170766167414805 0.0 28-29 0.0 0.0 0.0 0.02969409099562611 0.0 30-31 0.0 0.0 0.0 0.07285828375338099 0.0 32-33 0.0 0.0 0.0 0.14571656750676198 0.0 34-35 0.0 0.0 0.0 0.2558121992425612 0.0 36-37 0.0 0.0 0.0 0.41865076276696245 0.0 38-39 0.0 0.0 0.0 0.6600948534632529 0.0 40-41 0.0 0.0 0.0 0.9136919693049308 0.0 42-43 0.0 0.0 0.0 1.159566226802988 0.0 44-45 0.0 0.0 0.0 1.4290281291645064 0.0 46-47 0.0 0.0 0.0 1.7507540263630845 0.0 48-49 0.0 0.0 0.0 2.077029824601315 0.0 50-51 0.0 0.0 0.0 2.399414260108264 0.0 52-53 0.0 0.0 0.0 2.7103640758971386 0.0 54-55 0.0 0.0 0.0 3.0379569507521107 0.0 56-57 0.0 0.0 0.0 3.405900263774526 0.0 58-59 0.0 0.0 0.0 3.8000055077749426 0.0 60-61 0.0 0.0 0.0 4.203030952497835 0.0 62-63 0.0 0.0 0.0 4.577499781485017 0.0 64-65 0.0 0.0 0.0 4.9607092098378445 0.0 66-67 0.0 0.0 0.0 5.394626087935219 0.0 68-69 0.0 0.0 0.0 5.846922171548032 0.0 70-71 0.0 0.0 0.0 6.351841452711561 0.0 72-73 0.0 0.0 0.0 6.820481259795757 0.0 74-75 0.0 1.1973423788558915E-4 0.0 7.288522395690526 0.0 76-77 0.0 1.1973423788558915E-4 0.0 7.775601275409102 0.0 78-79 0.0 1.1973423788558915E-4 0.0 8.305844347885433 0.0 80-81 0.0 1.1973423788558915E-4 0.0 8.88200550059089 0.0 82-83 0.0 1.1973423788558915E-4 0.0 9.44266106949016 0.0 84-85 0.0 1.1973423788558915E-4 0.0 9.986733446442276 0.0 86-87 0.0 1.1973423788558915E-4 0.0 10.571275995799724 0.0 88-89 0.0 1.1973423788558915E-4 0.0 11.167672234707842 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 25 5.270171E-5 76.01255 2 GTACACG 60 1.4919406E-6 47.499313 3 CACGCAG 40 5.389305E-4 47.496468 5 ATCACGC 50 0.0016156778 37.99945 3 CGTGCAA 135 3.092282E-10 35.182568 9 GGTATCA 5505 0.0 34.17458 1 CCGTGCA 140 4.5656634E-10 33.92605 8 CTGACCG 140 4.5656634E-10 33.92605 4 TGACCGT 140 4.5656634E-10 33.92605 5 GTATGGT 85 1.6298269E-5 33.53495 1 ACCGTGC 145 6.6393113E-10 32.756184 7 TGGTACT 190 0.0 32.505367 2 GTATCAA 8435 0.0 32.272667 1 GTGGTAC 195 0.0 31.671896 1 TCACGCA 60 0.0039517777 31.66431 4 GCAGATC 150 9.549694E-10 31.66431 8 CATATAG 200 0.0 30.874554 3 TACTCGC 110 2.7338538E-6 30.225025 7 TCCTGAC 160 1.900844E-9 29.692404 2 GACCGTG 160 1.906301E-9 29.685293 6 >>END_MODULE