FastQCFastQC Report
Thu 26 May 2016
SRR938212_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938212_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838704
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139531.6636381846277113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103761.2371468360708904No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80080.9548064633052901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78240.9328678532593143No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29180.3479177397508537No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24710.29462122512829314No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23570.2810288254258952No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18220.21723993208569412No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17150.2044821534176539No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15250.18182815391365725No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13490.16084339647837617No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA12950.15440489135618765No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT11880.14164711268814742No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT11850.14128941795913696No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA11150.13294320761555925No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10900.12996241820713864No Hit
GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10840.1292470287491177No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT10770.1284124077147599No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT10460.12471622884831836No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT9820.1170854079627616No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG9670.11529693431770922No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT9430.11243537648562543No Hit
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9240.11016997653522577No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC9220.10993151338255212No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG9180.10945458707720483No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG9050.10790457658482612No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC8930.10647379766878422No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC8870.10575840821076327No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA8840.10540071348175281No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA8760.1044468608710582No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA8620.10277761880234267No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA8610.10265838722600584No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG8590.10241992407333218No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8390.10003529254659571No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAG608.2279075E-539.632913
GGTATCA58200.036.0029951
CATTCCG2150.035.3783049
GCGAAAT352.0631534E-433.90214548-49
GTATCAA90050.032.7352641
AGGGTCT1605.2750693E-1132.699115
CCTTTAG2350.032.4235841
GACTGTA600.003900989231.7480931
GGGACAC753.0616584E-431.6949627
TATGCCG601.5030128E-731.67507794-95
AGCGAAA452.4265057E-531.64200248-49
GACAATG4250.031.3220777
GGACAAT4400.030.2560866
GAGCGAA404.5150644E-429.6643846-47
GGATGCC1652.6211637E-928.8118788
GTATGGT856.3589925E-427.9761753
TAGGCAT2750.027.6726345
CTTTAGG2750.027.670982
GGCATTC2750.027.6610557
GGTATGG1056.783969E-527.2126521
Produced by FastQC (version 0.11.5)