##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938212_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 838704 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13739412236021 33.0 31.0 34.0 31.0 34.0 2 32.2408442072531 34.0 31.0 34.0 31.0 34.0 3 32.226294377992716 34.0 31.0 34.0 30.0 34.0 4 35.749765113794616 37.0 35.0 37.0 35.0 37.0 5 35.781461636048 37.0 35.0 37.0 35.0 37.0 6 35.85045141074801 37.0 37.0 37.0 35.0 37.0 7 35.80514460405578 37.0 37.0 37.0 35.0 37.0 8 35.81600183139701 37.0 37.0 37.0 35.0 37.0 9 37.484598857284574 39.0 38.0 39.0 35.0 39.0 10-11 37.42347896278067 39.0 38.0 39.0 35.0 39.0 12-13 37.332135055991145 39.0 37.5 39.0 35.0 39.0 14-15 38.53533427764742 40.0 38.0 41.0 34.5 41.0 16-17 38.42282676605811 40.0 38.0 41.0 34.0 41.0 18-19 38.653118978805395 40.0 38.5 41.0 35.0 41.0 20-21 38.69944104237013 40.0 39.0 41.0 35.0 41.0 22-23 38.57543126061161 40.0 38.5 41.0 35.0 41.0 24-25 38.480737542684906 40.0 38.0 41.0 35.0 41.0 26-27 38.298080133157825 40.0 38.0 41.0 34.0 41.0 28-29 38.1433282779145 40.0 38.0 41.0 34.0 41.0 30-31 37.88126800396803 40.0 37.5 41.0 34.0 41.0 32-33 37.530267531810985 40.0 36.0 41.0 33.5 41.0 34-35 37.3159404271352 40.0 35.5 41.0 33.0 41.0 36-37 37.066202140445256 40.0 35.0 41.0 33.0 41.0 38-39 36.79828699994277 39.0 35.0 41.0 32.5 41.0 40-41 36.65804860832904 39.0 35.0 41.0 31.5 41.0 42-43 36.42656884908144 39.5 35.0 41.0 31.0 41.0 44-45 36.05419969381331 39.0 35.0 41.0 30.0 41.0 46-47 35.611103559777945 39.0 35.0 41.0 28.0 41.0 48-49 35.19227284000076 39.0 35.0 41.0 25.5 41.0 50-51 34.48687975733989 38.5 34.5 40.5 23.5 40.5 52-53 34.57988873309296 38.5 35.0 40.0 24.0 41.0 54-55 34.75722602968389 39.0 35.0 40.0 23.5 41.0 56-57 34.56167432133387 39.0 35.0 40.0 22.5 41.0 58-59 34.28899409088308 38.5 35.0 40.0 19.5 41.0 60-61 33.93808006161888 38.0 34.5 40.0 16.0 41.0 62-63 33.51596153112421 38.0 34.0 40.0 11.0 41.0 64-65 33.13129661954635 37.5 33.5 40.0 7.5 41.0 66-67 32.69477551078808 37.0 33.0 39.5 2.0 41.0 68-69 32.1466578196837 36.0 32.5 39.0 2.0 40.5 70-71 31.687035593010165 36.0 31.5 39.0 2.0 40.0 72-73 31.06638993017799 35.0 31.0 38.0 2.0 40.0 74-75 30.4643169699918 35.0 30.5 37.0 2.0 39.5 76-77 29.832102863465536 35.0 29.5 37.0 2.0 39.0 78-79 29.17577357446727 34.0 29.0 36.0 2.0 38.5 80-81 28.5212250090616 34.0 28.5 36.0 2.0 37.5 82-83 27.864803911749554 34.0 26.5 35.0 2.0 37.0 84-85 27.201977097998817 33.5 26.0 35.0 2.0 36.5 86-87 26.599300826036362 33.0 25.0 35.0 2.0 36.0 88-89 25.819928127205785 32.5 22.0 35.0 2.0 35.5 90-91 25.042126304393445 32.0 19.0 34.5 2.0 35.0 92-93 24.22832131479044 31.0 9.0 34.0 2.0 35.0 94-95 23.286156975524143 31.0 2.0 34.0 2.0 35.0 96-97 22.15751743165646 30.5 2.0 34.0 2.0 35.0 98-99 20.746191743451803 29.0 2.0 34.0 2.0 35.0 100-101 16.389263077319292 14.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.41047799619506975 1101 2 0.2946767691731367 1101 3 0.19491095647295253 1101 4 0.057482216279147735 1101 5 0.08532147970026926 1101 6 0.13770716372544456 1101 7 0.24211661308159904 1101 8 0.19662569990443757 1101 9 0.24708267810573403 1101 10-11 0.28913570169086 1101 12-13 0.27983484513316625 1101 14-15 0.4008377863048622 1101 16-17 0.4294353230975858 1101 18-19 0.35643253430075106 1101 20-21 0.3422991052324633 1101 22-23 0.3274175549931897 1101 24-25 0.388980382372452 1101 26-27 0.37670822875722365 1101 28-29 0.5506372533745676 1101 30-31 0.5053564492403027 1101 32-33 0.6254856211633992 1101 34-35 0.6399844678479951 1101 36-37 0.5138286128105278 1101 38-39 0.39321248206407944 1101 40-41 0.3678531233332123 1101 42-43 0.2692258187673886 1101 44-45 0.5524835075170387 1101 46-47 0.46079003922708495 1101 48-49 0.8630467621378486 1101 50-51 0.8275829504992132 1101 52-53 0.7770977228044842 1101 54-55 0.707026904617237 1101 56-57 0.8577557675714829 1101 58-59 0.9137595849374449 1101 60-61 0.9191996788657804 1101 62-63 0.7396874152436013 1101 64-65 0.8542513481196963 1101 66-67 0.7896981666699361 1101 68-69 0.6640360414471331 1101 70-71 0.533481031725163 1101 72-73 0.66665883553231 1101 74-75 0.7342201991548691 1101 76-77 0.5111040990831413 1101 78-79 0.576665244090762 1101 80-81 0.48865111281129714 1101 82-83 0.3987709554358503 1101 84-85 0.2996015063059936 1101 86-87 0.46054709422094575 1101 88-89 0.2869989161455351 1101 90-91 0.2336213672532068 1101 92-93 0.3327459253067211 1101 94-95 0.20948593080918698 1101 96-97 -0.07657862336488108 1101 98-99 0.20264903619382224 1101 100-101 0.08006549853591238 1102 1 0.18290354478440918 1102 2 0.31031352465902984 1102 3 0.2248325865669969 1102 4 0.10915149527600931 1102 5 0.1194104295435352 1102 6 0.057748307926068776 1102 7 0.20318832154555366 1102 8 0.2664356528824996 1102 9 0.2434130072593419 1102 10-11 0.19628401673788431 1102 12-13 0.17629644387924515 1102 14-15 0.11067712799764706 1102 16-17 0.26938144378724616 1102 18-19 0.18100345906564286 1102 20-21 0.181775006486383 1102 22-23 0.24280450640384998 1102 24-25 0.21509225541321086 1102 26-27 0.26087065038731794 1102 28-29 0.25635433801407004 1102 30-31 0.2863635025318345 1102 32-33 0.07168566034835777 1102 34-35 0.15733850389814563 1102 36-37 0.25183409870394513 1102 38-39 0.10026773284778301 1102 40-41 0.09253768446173183 1102 42-43 0.18034748804638667 1102 44-45 0.2704586250719032 1102 46-47 0.35661292323962357 1102 48-49 0.25084064928517336 1102 50-51 0.25714211977820867 1102 52-53 0.25375034819633413 1102 54-55 0.12842678706237365 1102 56-57 0.15489330675644197 1102 58-59 0.05790241409104624 1102 60-61 0.05282624626389776 1102 62-63 0.07224129455394745 1102 64-65 0.004380658464519627 1102 66-67 0.06979123093325512 1102 68-69 0.2192358847073237 1102 70-71 0.033949291913248913 1102 72-73 -0.04081863311972711 1102 74-75 -0.09543987137804777 1102 76-77 -0.35058575201403386 1102 78-79 -0.270265594467233 1102 80-81 -0.37731675552726074 1102 82-83 -0.5243922498933067 1102 84-85 -0.27293376015168747 1102 86-87 -0.3534585875972347 1102 88-89 -0.4307420713152794 1102 90-91 -0.5405851374489892 1102 92-93 -0.796904348987951 1102 94-95 -1.1484098372158869 1102 96-97 -1.0684918286940324 1102 98-99 -1.1712332130224787 1102 100-101 -1.8575020955079715 1103 1 0.13325580800589876 1103 2 0.20289090173868374 1103 3 0.22477496039356026 1103 4 0.18182367670551258 1103 5 0.22266909670590707 1103 6 0.07443759131672323 1103 7 0.049107087230098045 1103 8 0.1687258285493769 1103 9 0.3234464676181119 1103 10-11 0.3218616726538599 1103 12-13 0.11657063293026937 1103 14-15 0.3409336574148796 1103 16-17 0.46508643495742064 1103 18-19 0.3783442899978837 1103 20-21 0.39069219209843453 1103 22-23 0.37256005989394936 1103 24-25 0.24733502890516945 1103 26-27 0.26861393507920894 1103 28-29 0.39176840995384055 1103 30-31 0.5432684193456652 1103 32-33 0.469363883231523 1103 34-35 0.2505674285193962 1103 36-37 0.3582677821317688 1103 38-39 0.3252412433970946 1103 40-41 0.5289081649896659 1103 42-43 0.5044909958073305 1103 44-45 0.40440973576992434 1103 46-47 0.28266274199601327 1103 48-49 0.1435769630689876 1103 50-51 0.07045004802432686 1103 52-53 0.015307184333522628 1103 54-55 0.007602361000472513 1103 56-57 -0.05508345688330252 1103 58-59 -0.018236632288726184 1103 60-61 0.18321661711620862 1103 62-63 0.03136110122742508 1103 64-65 -0.11163477047868753 1103 66-67 -0.20394424634157815 1103 68-69 -0.02481932488464622 1103 70-71 -0.4157616477651622 1103 72-73 -0.19212079874850474 1103 74-75 0.1230897317449191 1103 76-77 0.028909554241192126 1103 78-79 0.018428522086352928 1103 80-81 0.012626547753711748 1103 82-83 0.02721105896439724 1103 84-85 0.00407804391932487 1103 86-87 -0.5072591266807898 1103 88-89 -0.35423867880668425 1103 90-91 -0.2804262007448308 1103 92-93 -0.38294301429141697 1103 94-95 -0.5777062402273216 1103 96-97 -1.0981553661975205 1103 98-99 -0.9927017509906939 1103 100-101 -0.6192020676243413 1104 1 0.22113465823532152 1104 2 0.12986428363068825 1104 3 0.2377342273496552 1104 4 0.14210227179614776 1104 5 0.21585510796767693 1104 6 0.2281861424439242 1104 7 0.2233181802803088 1104 8 0.23286716994839196 1104 9 0.3359496397064774 1104 10-11 0.2683836920910423 1104 12-13 0.39940603412229336 1104 14-15 0.2353494101771645 1104 16-17 0.3996762582120894 1104 18-19 0.3479351077451156 1104 20-21 0.2743142712051707 1104 22-23 0.3941662682710003 1104 24-25 0.30372961612216187 1104 26-27 0.2812251296362476 1104 28-29 0.24083358693922463 1104 30-31 0.3167504464644466 1104 32-33 0.3345364105457733 1104 34-35 0.4781159014589065 1104 36-37 0.49467958435806025 1104 38-39 0.4547618979789618 1104 40-41 0.4228026664745528 1104 42-43 0.4953365202478466 1104 44-45 0.6019879749008012 1104 46-47 0.636463761179435 1104 48-49 0.6681083513120285 1104 50-51 0.6614186837862803 1104 52-53 0.8732747844514961 1104 54-55 0.8445055797271124 1104 56-57 0.747209705808821 1104 58-59 0.908021743739198 1104 60-61 0.8827341167561329 1104 62-63 1.074949024658359 1104 64-65 0.6550624168219841 1104 66-67 0.7233000490432886 1104 68-69 0.6872476421873586 1104 70-71 0.4461393419264077 1104 72-73 0.5151057329415174 1104 74-75 0.3487366663073068 1104 76-77 0.4454939180745825 1104 78-79 0.33247898744028603 1104 80-81 0.10559102722258729 1104 82-83 0.0877293610569474 1104 84-85 0.2548770672590379 1104 86-87 0.16567627245365557 1104 88-89 0.2742943417758852 1104 90-91 0.12311144937210727 1104 92-93 -0.25632524922485445 1104 94-95 -0.3559995538079761 1104 96-97 -0.13054324573360887 1104 98-99 0.08570370020029827 1104 100-101 -0.7967001300784737 1105 1 0.22005228384771414 1105 2 0.05519943991036058 1105 3 0.24551023281849638 1105 4 0.18991663509416412 1105 5 0.16483159955554072 1105 6 0.12295846404204269 1105 7 0.23984812597169736 1105 8 0.2366175022563155 1105 9 0.27373210144359206 1105 10-11 0.2780158746895083 1105 12-13 0.31936729651263107 1105 14-15 0.2856608301764112 1105 16-17 0.3948815295652466 1105 18-19 0.4922944180637572 1105 20-21 0.326515099126631 1105 22-23 0.5244119862460153 1105 24-25 0.3231553880260023 1105 26-27 0.5036198354962593 1105 28-29 0.43777825292229267 1105 30-31 0.37816569844880377 1105 32-33 0.3453981324704998 1105 34-35 0.25039477427042556 1105 36-37 0.35008765849121204 1105 38-39 0.2807986987081321 1105 40-41 0.19464953898223314 1105 42-43 0.19147889995237222 1105 44-45 0.2954766384532732 1105 46-47 0.32253246346950704 1105 48-49 0.33903330668781706 1105 50-51 0.550636715763801 1105 52-53 0.38825712465884976 1105 54-55 0.29853315179045836 1105 56-57 0.230769957983064 1105 58-59 0.3698632993200732 1105 60-61 0.22945945950802127 1105 62-63 0.2279103630047956 1105 64-65 0.12150881080626164 1105 66-67 -0.08351036453765914 1105 68-69 -0.07444484955240682 1105 70-71 -0.03937203249917687 1105 72-73 -0.2614456703473813 1105 74-75 -0.6093213261730384 1105 76-77 -0.7980355718802201 1105 78-79 -0.6919314033538342 1105 80-81 -0.46294036163465435 1105 82-83 -0.4298734645098783 1105 84-85 -0.5373925009305509 1105 86-87 -0.31607052789574297 1105 88-89 -0.05150034889290822 1105 90-91 -0.14747740027280187 1105 92-93 -0.6184886687683573 1105 94-95 -0.770373295785113 1105 96-97 -0.9734755308727934 1105 98-99 -0.8731898093511816 1105 100-101 -1.2512926256160526 1106 1 -0.00104403759637961 1106 2 0.07076309183106133 1106 3 0.1400786528542426 1106 4 0.1307154151191412 1106 5 0.13552597787200682 1106 6 0.15577116889502918 1106 7 0.07153401000506676 1106 8 -0.05207599282031339 1106 9 0.21285583092379312 1106 10-11 0.09371287512421844 1106 12-13 0.2485218013808037 1106 14-15 0.17811602990591524 1106 16-17 -0.21273917412849386 1106 18-19 0.1559396109619513 1106 20-21 0.31634576548967885 1106 22-23 0.31818066021320845 1106 24-25 0.3272162211695786 1106 26-27 0.35490001573238317 1106 28-29 0.213569891125168 1106 30-31 0.07734059373657942 1106 32-33 0.16593311359815033 1106 34-35 0.13439623023609215 1106 36-37 0.13590889902916103 1106 38-39 0.0727551784453695 1106 40-41 0.13291072956192806 1106 42-43 0.24287845557233112 1106 44-45 0.525572690785232 1106 46-47 0.5840326946299328 1106 48-49 0.6369758063945596 1106 50-51 0.6171488154595011 1106 52-53 0.4396195911060943 1106 54-55 0.3575290875071673 1106 56-57 0.4350769597288391 1106 58-59 0.4296475955918382 1106 60-61 0.838091921332051 1106 62-63 0.6118376406250192 1106 64-65 0.5095478113651879 1106 66-67 0.23581447451257986 1106 68-69 0.1756163907095214 1106 70-71 0.22047471158898446 1106 72-73 0.3042547802429425 1106 74-75 0.6697884991800862 1106 76-77 0.0921537381576627 1106 78-79 0.19152406548781187 1106 80-81 0.21350059585252978 1106 82-83 0.15133112281701244 1106 84-85 0.16341518049588188 1106 86-87 0.22898246649430476 1106 88-89 0.011775504848095864 1106 90-91 -0.017590254862540178 1106 92-93 0.3403121606490487 1106 94-95 0.47006546050279496 1106 96-97 0.2564687146126765 1106 98-99 -0.45253217519188027 1106 100-101 -1.1954130429468748 1107 1 0.1420718787061901 1107 2 0.05896049810063886 1107 3 0.06230934306928759 1107 4 0.09825039292616111 1107 5 0.07237578986678272 1107 6 0.060629324755538505 1107 7 0.09059014964174139 1107 8 0.18384807295232264 1107 9 0.14070695414255852 1107 10-11 0.16325902752600285 1107 12-13 0.0786086324948343 1107 14-15 0.11465578713224289 1107 16-17 0.13820416553216575 1107 18-19 0.23559370660399281 1107 20-21 0.22303505905395582 1107 22-23 0.08486484142405004 1107 24-25 0.23114105695100307 1107 26-27 0.10573511235428867 1107 28-29 0.1566752355857588 1107 30-31 0.18103063995288693 1107 32-33 0.1758745897744305 1107 34-35 0.1051122279576191 1107 36-37 0.1351215334848419 1107 38-39 0.1753044124601999 1107 40-41 0.3790050651058863 1107 42-43 0.2611030488325454 1107 44-45 0.4088729673887954 1107 46-47 0.3630614737084201 1107 48-49 0.24995013890896445 1107 50-51 0.16499665667357988 1107 52-53 0.1329296663870707 1107 54-55 0.16848798513228047 1107 56-57 0.06959418157500608 1107 58-59 0.023956315468005585 1107 60-61 0.045662149131580065 1107 62-63 0.2569566842993467 1107 64-65 0.1574723378807903 1107 66-67 0.31688314572671317 1107 68-69 0.3487145242271552 1107 70-71 0.14610417010554855 1107 72-73 0.33350406901043783 1107 74-75 0.2741941170884701 1107 76-77 0.12178724237613991 1107 78-79 0.014048084870573518 1107 80-81 -0.14546629850321224 1107 82-83 0.024734893019878967 1107 84-85 0.2126839088295256 1107 86-87 0.12442537144713484 1107 88-89 0.3000609572045896 1107 90-91 0.020503089768464378 1107 92-93 0.007789072219537729 1107 94-95 0.06581479108429633 1107 96-97 0.12443508668786407 1107 98-99 -0.05867662442258492 1107 100-101 -0.31864995161404863 1108 1 -0.16164726691007303 1108 2 -0.06998225909187283 1108 3 -0.044301732712177966 1108 4 -0.0897626970214418 1108 5 -0.03569279062146791 1108 6 0.032181642526687426 1108 7 -0.04985961975391717 1108 8 -0.05006512649627837 1108 9 0.018433347144529932 1108 10-11 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-0.21016137507898236 2316 64-65 -0.2346023385162468 2316 66-67 -0.0410366730870777 2316 68-69 -0.06374202725834976 2316 70-71 -0.1518889410440103 2316 72-73 -0.20424148390631558 2316 74-75 -0.14444352623264933 2316 76-77 -0.06106088311208424 2316 78-79 -0.1486352115020324 2316 80-81 -0.18020885786318885 2316 82-83 -0.7545209643379245 2316 84-85 -0.5237934455163753 2316 86-87 -0.48396550003963057 2316 88-89 -0.7565167415838303 2316 90-91 -0.5208965946236255 2316 92-93 -0.6388207640523049 2316 94-95 -0.45657764271406265 2316 96-97 -0.5631366721709163 2316 98-99 -0.6809201667806235 2316 100-101 -0.7086160316274714 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5128.0 3 842.0 4 618.0 5 539.0 6 615.0 7 649.0 8 757.0 9 904.0 10 1228.0 11 1857.0 12 2975.0 13 4399.0 14 5325.0 15 7405.0 16 9245.0 17 12901.0 18 11453.0 19 7098.0 20 6526.0 21 6271.0 22 6783.0 23 7517.0 24 8457.0 25 9590.0 26 10765.0 27 12583.0 28 14446.0 29 17376.0 30 21156.0 31 25514.0 32 32135.0 33 42165.0 34 59723.0 35 92515.0 36 112399.0 37 160063.0 38 110335.0 39 8447.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.351937182902496 24.370163311557665 15.88732374184905 19.39057576369079 2 13.9128505322328 24.313668415922965 41.80738457353975 19.96609647830449 3 15.76335973576078 32.03745299567841 32.59081730499401 19.608369963566798 4 9.970081398652827 21.031964909515928 42.78622645969585 26.211727232135395 5 9.199002710648704 42.070247494531515 36.708204461042044 12.02254533377774 6 23.164347360674384 44.31240382685488 19.143951485725754 13.379297326744974 7 22.8615649552817 37.76304617049202 24.827283117156085 14.548105757070196 8 23.442171903374447 36.63022805749044 22.087118581781652 17.840481457353462 9 26.186184537224495 17.831205355437564 24.183863974941747 31.798746132396193 10-11 21.367273491099333 30.4118755941288 29.711840446170218 18.509010468601655 12-13 23.007366436323732 30.431358641203566 29.45446099872589 17.106813923746817 14-15 21.026391333857447 31.103163844033787 28.797362000801098 19.07308282130767 16-17 16.563536056713676 34.290201537467865 30.261541455401137 18.884720950417318 18-19 16.318099032407687 32.067127947950944 34.9331329181364 16.681640101504964 20-21 17.251070312294804 31.3975186424166 35.112511132919295 16.238899912369302 22-23 17.670513125308375 31.031172682059672 34.62732678093345 16.67098741169851 24-25 17.094099308559866 32.16621568415412 34.76325359835054 15.976431408935488 26-27 16.32293378166673 32.126029288143364 35.30368776508349 16.247349165106414 28-29 16.228397950461527 31.15148056710901 36.08234684570681 16.53777463672265 30-31 16.742920943599685 31.58424899134896 35.27083048635449 16.40199957869686 32-33 16.210984482432387 32.4654828579734 35.06676607014631 16.25676658944791 34-35 16.786446964697234 31.733166412665604 35.10065960714767 16.37972701548949 36-37 17.34580630379058 31.03767190311848 34.417558104566744 17.1989636885242 38-39 16.27989924781804 32.44826251394946 34.59732826590178 16.674509972330725 40-41 16.044029591665083 31.6561859870271 35.882041352455055 16.417743068852754 42-43 16.21228399101157 31.50174453598445 34.81000992020291 17.47596155280107 44-45 16.183696314015116 31.70995419281004 34.604376998958145 17.5019724942167 46-47 16.97251290554424 31.4733046841683 34.093778797033295 17.460403613254165 48-49 16.559169026843694 31.80534607679834 33.828866142925044 17.806618753432918 50-51 16.47177762071341 31.540950557528802 33.78669267175436 18.200579150003428 52-53 16.550666009939167 32.02959805653183 34.018385189761254 17.401350743767747 54-55 17.062395510944402 31.50277553946622 33.564255513055805 17.87057343653358 56-57 17.007027450886856 32.03599295167845 32.99550684408719 17.961472753347504 58-59 16.60716117657444 31.007171674034772 33.87184509565811 18.51382205373268 60-61 17.216703761220806 32.00287930076169 32.93244974343927 17.84796719457823 62-63 16.956832674348625 31.614684513431897 33.75101173885477 17.67747107336471 64-65 17.080965930269382 30.95701430192746 33.593934040925184 18.368085726877975 66-67 16.861809379374012 31.61705551086082 32.73739911629663 18.78373599346854 68-69 16.792644057746582 30.95369190012402 33.481491732976046 18.772172309153348 70-71 17.266949594844068 30.950095883424844 33.5872926875095 18.195661834221585 72-73 17.3318016410818 30.51509116491518 33.09594489248033 19.057162301522688 74-75 16.84695253562229 31.423955187873243 32.62823338138438 19.100858895120084 76-77 16.910444130140792 30.819354638668727 33.37409872897829 18.896102502212184 78-79 16.801864707348514 30.47453677478137 33.15335417156118 19.570244346308925 80-81 17.134992763868357 31.025443634201764 32.21643874988745 19.623124852042427 82-83 17.601573223713316 30.568325873877356 32.37034201396963 19.45975888843969 84-85 16.883678304023793 30.16929829425693 32.5420860664755 20.404937335243776 86-87 17.01088836149837 30.494746633910154 32.92870687290551 19.565658131685968 88-89 17.712755835241776 29.707878493583873 33.02454240620692 19.554823264967432 90-91 17.307057639066684 29.61816453282582 33.269297220068786 19.80548060803871 92-93 17.93764673243462 29.80141340437147 32.77218773398883 19.488752129205086 94-95 17.54217554644605 28.817963080847825 33.25432285554566 20.385538517160466 96-97 18.108891375883207 28.577573144279345 32.76351865683217 20.550016823005283 98-99 17.64835356237041 28.883700732730954 32.72833122970435 20.739614475194276 100-101 18.266693728435154 27.808175837820414 33.570123807590825 20.355006626153607 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7536.0 1 4413.0 2 1754.5 3 2107.5 4 1525.5 5 1560.5 6 3257.0 7 6071.5 8 8295.5 9 8219.5 10 5949.5 11 3990.5 12 3583.5 13 3331.0 14 2939.5 15 2593.0 16 2358.0 17 2342.5 18 2405.5 19 2517.5 20 2758.0 21 3029.5 22 3339.5 23 3785.5 24 3991.5 25 4748.0 26 7260.5 27 9709.0 28 9990.0 29 11165.5 30 15131.5 31 18928.0 32 23991.5 33 31007.5 34 37284.5 35 42278.5 36 49036.5 37 54870.5 38 53024.5 39 48778.5 40 44157.5 41 38442.5 42 33246.0 43 31590.5 44 31147.0 45 28436.5 46 23803.5 47 19106.0 48 18931.5 49 17011.0 50 11749.5 51 7445.5 52 4783.5 53 3526.0 54 2747.5 55 2214.0 56 1910.0 57 1678.0 58 1445.0 59 1271.5 60 1162.5 61 1052.0 62 881.0 63 679.0 64 526.0 65 416.5 66 348.5 67 318.5 68 269.0 69 219.5 70 189.0 71 177.0 72 160.0 73 125.5 74 103.5 75 91.5 76 66.5 77 46.0 78 40.5 79 30.0 80 22.5 81 14.5 82 8.5 83 8.0 84 5.5 85 2.5 86 1.0 87 1.0 88 1.0 89 0.5 90 1.0 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14605868101260994 2 0.05281958831721323 3 0.1527356492874721 4 0.13091627081783325 5 0.19494362731070794 6 0.19935519563517046 7 0.16108185963104982 8 0.06939277742803182 9 0.11637001850474063 10-11 0.09747181365535397 12-13 0.1498740914553883 14-15 0.13109511818233846 16-17 0.13032011293614912 18-19 0.13228743394570672 20-21 0.13091627081783325 22-23 0.1975667219901181 24-25 0.21789570575554665 26-27 0.1468933020469677 28-29 0.1254316183063393 30-31 0.09866412941872223 32-33 0.12406045517846583 34-35 0.09311986111905989 36-37 0.0872775138785555 38-39 0.048825330509929606 40-41 0.05901963028672809 42-43 0.061463877601633 44-45 0.035590625536542096 46-47 0.04787147789923501 48-49 0.03737909918159446 50-51 0.04846763578091913 52-53 0.02420400999637536 54-55 0.04751378317022454 56-57 0.024621320513554246 58-59 0.04840801999275072 60-61 0.036484862359068275 62-63 0.050017646273297856 64-65 0.03821372021595223 66-67 0.03582908868921574 68-69 0.06253696178866441 70-71 0.05323689883439211 72-73 0.04954071996795055 74-75 0.042863751693088384 76-77 0.019792441671912856 78-79 0.02158091531696522 80-81 0.02426362578454377 82-83 0.020388599553596978 84-85 0.018421278544039375 86-87 0.02313092580934394 88-89 0.06468313016272725 90-91 0.10677187660962628 92-93 0.07827552986512523 94-95 0.07505627730403099 96-97 0.06748507220664264 98-99 0.05603884087830749 100-101 0.1328239760392224 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 838704.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.687514775834593 #Duplication Level Percentage of deduplicated Percentage of total 1 76.47882222714246 15.056779426352943 2 8.872098944775653 3.493391581358743 3 3.4161577629509643 2.0176696930403746 4 1.8402306109964177 1.4491826937974035 5 1.1999079737001255 1.1811602980931482 6 0.8425063970703226 0.9952114284634285 7 0.6606505503343915 0.9104597229960111 8 0.5270688486745432 0.8301340596929755 9 0.4149536274641415 0.7352465104787804 >10 4.040057798664704 17.47107041786132 >50 0.8564906884205309 11.884145653266632 >100 0.7888412123252562 29.69716454774849 >500 0.05073710707145599 6.739399134996028 >1k 0.009060197691331427 2.763232129369776 >5k 0.00120802635884419 1.8826651922443645 >10k+ 0.00120802635884419 2.893087510239587 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13953 1.6636381846277113 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10376 1.2371468360708904 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8008 0.9548064633052901 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7824 0.9328678532593143 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2918 0.3479177397508537 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2471 0.29462122512829314 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2357 0.2810288254258952 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1822 0.21723993208569412 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1715 0.2044821534176539 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1525 0.18182815391365725 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1349 0.16084339647837617 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1295 0.15440489135618765 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1188 0.14164711268814742 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1185 0.14128941795913696 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1115 0.13294320761555925 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1090 0.12996241820713864 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1084 0.1292470287491177 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1077 0.1284124077147599 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 1046 0.12471622884831836 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 982 0.1170854079627616 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 967 0.11529693431770922 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 943 0.11243537648562543 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 924 0.11016997653522577 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 922 0.10993151338255212 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 918 0.10945458707720483 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 905 0.10790457658482612 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 893 0.10647379766878422 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 887 0.10575840821076327 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 884 0.10540071348175281 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 876 0.1044468608710582 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 862 0.10277761880234267 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 861 0.10265838722600584 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 859 0.10241992407333218 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 839 0.10003529254659571 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.1923157633682444E-4 0.0 14-15 0.0 0.0 0.0 1.1923157633682444E-4 0.0 16-17 0.0 0.0 0.0 1.1923157633682444E-4 0.0 18-19 0.0 0.0 0.0 2.9807894084206107E-4 0.0 20-21 0.0 0.0 0.0 6.557736698525345E-4 0.0 22-23 0.0 0.0 0.0 0.002086552585894428 0.0 24-25 0.0 0.0 0.0 0.0059019630286728094 0.0 26-27 0.0 0.0 0.0 0.014427020736755756 0.0 28-29 0.0 0.0 0.0 0.030225204601384996 0.0 30-31 0.0 0.0 0.0 0.07553320360937828 0.0 32-33 0.0 0.0 0.0 0.1517221808886091 0.0 34-35 0.0 0.0 0.0 0.2661248783837922 0.0 36-37 0.0 0.0 0.0 0.4372818062153036 0.0 38-39 0.0 0.0 0.0 0.672644937904195 0.0 40-41 0.0 0.0 0.0 0.9183812167343901 0.0 42-43 0.0 0.0 0.0 1.1615540166733438 0.0 44-45 0.0 0.0 0.0 1.4307193002537248 0.0 46-47 0.0 0.0 0.0 1.7533599458211717 0.0 48-49 0.0 0.0 0.0 2.082558328087144 0.0 50-51 0.0 0.0 0.0 2.4131874892691583 0.0 52-53 0.0 0.0 0.0 2.7221165035578703 0.0 54-55 0.0 0.0 0.0 3.050241801636811 0.0 56-57 0.0 0.0 0.0 3.420038535645472 0.0 58-59 0.0 0.0 0.0 3.81248926915813 0.0 60-61 0.0 0.0 0.0 4.222526660180469 0.0 62-63 0.0 0.0 0.0 4.601384993990728 0.0 64-65 0.0 0.0 0.0 4.999022301074038 0.0 66-67 0.0 0.0 0.0 5.420684770789218 0.0 68-69 0.0 0.0 0.0 5.855999255994964 0.0 70-71 0.0 0.0 0.0 6.3438948663652495 0.0 72-73 0.0 0.0 0.0 6.809971098265896 0.0 74-75 0.0 0.0 0.0 7.280399282702836 0.0 76-77 0.0 0.0 0.0 7.775031477136153 0.0 78-79 0.0 1.1923157633682444E-4 0.0 8.32033709151262 0.0 80-81 0.0 1.1923157633682444E-4 0.0 8.898312157805375 0.0 82-83 0.0 1.1923157633682444E-4 0.0 9.44934088784601 0.0 84-85 0.0 1.1923157633682444E-4 0.0 10.00054846525115 0.0 86-87 0.0 1.1923157633682444E-4 0.0 10.565884984452204 0.0 88-89 0.0 1.1923157633682444E-4 0.0 11.148748545374769 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTAGAG 60 8.2279075E-5 39.63291 3 GGTATCA 5820 0.0 36.002995 1 CATTCCG 215 0.0 35.378304 9 GCGAAAT 35 2.0631534E-4 33.902145 48-49 GTATCAA 9005 0.0 32.735264 1 AGGGTCT 160 5.2750693E-11 32.69911 5 CCTTTAG 235 0.0 32.423584 1 GACTGTA 60 0.0039009892 31.748093 1 GGGACAC 75 3.0616584E-4 31.694962 7 TATGCCG 60 1.5030128E-7 31.675077 94-95 AGCGAAA 45 2.4265057E-5 31.642002 48-49 GACAATG 425 0.0 31.322077 7 GGACAAT 440 0.0 30.256086 6 GAGCGAA 40 4.5150644E-4 29.66438 46-47 GGATGCC 165 2.6211637E-9 28.811878 8 GTATGGT 85 6.3589925E-4 27.976175 3 TAGGCAT 275 0.0 27.672634 5 CTTTAGG 275 0.0 27.67098 2 GGCATTC 275 0.0 27.661055 7 GGTATGG 105 6.783969E-5 27.212652 1 >>END_MODULE