##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938212_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 838704 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.531930216143 34.0 31.0 34.0 31.0 34.0 2 32.73305003910796 34.0 31.0 34.0 31.0 34.0 3 32.766058108701046 34.0 31.0 34.0 31.0 34.0 4 36.23599863599077 37.0 35.0 37.0 35.0 37.0 5 36.233577042675364 37.0 37.0 37.0 35.0 37.0 6 36.281361481523874 37.0 37.0 37.0 35.0 37.0 7 36.242624334687804 37.0 37.0 37.0 35.0 37.0 8 36.24349114824777 37.0 37.0 37.0 35.0 37.0 9 37.910435624487306 39.0 38.0 39.0 35.0 39.0 10-11 37.915584640111405 39.0 38.0 39.0 35.0 39.0 12-13 37.85793378832103 39.0 38.0 39.0 35.0 39.0 14-15 39.07277716572236 40.0 38.5 41.0 35.5 41.0 16-17 38.998929300444495 40.0 38.0 41.0 36.0 41.0 18-19 39.29006300196494 40.5 39.0 41.0 36.0 41.0 20-21 39.31409829928079 41.0 39.0 41.0 36.0 41.0 22-23 39.18908518380739 41.0 39.0 41.0 35.0 41.0 24-25 39.083318429386296 40.5 39.0 41.0 35.0 41.0 26-27 38.87884641065263 40.0 38.0 41.0 35.0 41.0 28-29 38.755339786146244 40.0 38.0 41.0 35.0 41.0 30-31 38.45329162612793 40.0 37.5 41.0 35.0 41.0 32-33 38.14795744386578 40.0 37.0 41.0 34.0 41.0 34-35 37.94359094507716 40.0 36.0 41.0 34.0 41.0 36-37 37.84807751006315 40.0 36.0 41.0 34.0 41.0 38-39 37.67145441061447 40.0 36.0 41.0 33.0 41.0 40-41 37.51247877677941 40.0 36.0 41.0 33.0 41.0 42-43 37.24745559816097 40.0 36.0 41.0 33.0 41.0 44-45 36.90915925046262 40.0 35.0 41.0 32.5 41.0 46-47 36.4339296104466 40.0 35.0 41.0 31.0 41.0 48-49 36.00588228981858 40.0 35.0 41.0 30.0 41.0 50-51 35.688723912131096 39.0 35.0 41.0 28.5 41.0 52-53 35.30951324901277 39.0 35.0 40.5 27.0 41.0 54-55 35.07773183387703 39.0 35.0 40.0 26.0 41.0 56-57 34.810148753314635 38.5 35.0 40.0 25.0 41.0 58-59 34.46845728648009 38.0 34.5 40.0 23.0 41.0 60-61 34.10209740265934 38.0 34.0 40.0 22.0 41.0 62-63 33.71011286461016 38.0 34.0 40.0 20.5 41.0 64-65 33.28535633548904 37.0 33.0 40.0 18.0 41.0 66-67 32.79233317117839 36.5 33.0 39.0 10.5 40.5 68-69 32.26942222762739 36.0 32.0 39.0 7.5 40.0 70-71 31.784363136458154 35.5 31.5 38.5 3.5 40.0 72-73 31.267878774871708 35.0 31.0 38.0 2.0 40.0 74-75 30.70369224422442 35.0 30.5 37.0 2.0 39.0 76-77 29.236725352448538 33.5 28.5 35.5 2.0 38.0 78-79 29.93066445372861 34.0 30.0 36.0 2.0 39.0 80-81 29.61980984948206 34.0 30.0 36.0 2.0 37.5 82-83 29.110003052328352 34.0 29.0 35.0 2.0 37.0 84-85 28.569187699116732 34.0 29.0 35.0 2.0 36.5 86-87 28.06361183444934 34.0 28.0 35.0 2.0 36.0 88-89 27.424195544554458 33.0 26.0 35.0 2.0 36.0 90-91 26.990316607527802 33.0 26.0 35.0 2.0 35.0 92-93 26.316919914534807 32.5 25.0 35.0 2.0 35.0 94-95 25.678759133138747 32.0 23.5 34.5 2.0 35.0 96-97 24.81602269692287 32.0 17.0 34.0 2.0 35.0 98-99 23.622689888208473 31.0 2.0 34.0 2.0 35.0 100-101 19.232216014231483 25.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07648030590989663 1101 2 0.07941572410342701 1101 3 0.07500691618579935 1101 4 0.15334840178773845 1101 5 0.16452270447445017 1101 6 0.08506340138485058 1101 7 0.07131537291631673 1101 8 0.022948227454946846 1101 9 -0.08876180104564213 1101 10-11 0.10057052197169725 1101 12-13 0.1683618135806313 1101 14-15 0.09449656429235631 1101 16-17 0.20530721535924812 1101 18-19 0.1529220459454237 1101 20-21 0.29424928887073776 1101 22-23 0.33902357837171593 1101 24-25 0.3744908701551992 1101 26-27 0.3389929005016299 1101 28-29 0.491382793190823 1101 30-31 0.4427105536152922 1101 32-33 0.44576208117651817 1101 34-35 0.5498554350246536 1101 36-37 0.501053981509564 1101 38-39 0.47395288846657024 1101 40-41 0.680114451161792 1101 42-43 0.6046975448198424 1101 44-45 0.6210534022035006 1101 46-47 0.6663957202719217 1101 48-49 0.7137437573288778 1101 50-51 0.44089075918911647 1101 52-53 0.6104536009242665 1101 54-55 0.23116518672464537 1101 56-57 0.6315552105118698 1101 58-59 0.7495532891956955 1101 60-61 0.48039846135190345 1101 62-63 0.6821540002339574 1101 64-65 0.9965206836532801 1101 66-67 0.7059519543204402 1101 68-69 0.7308128247005357 1101 70-71 0.8393059961966323 1101 72-73 0.5948578145539827 1101 74-75 0.6510733680799028 1101 76-77 0.657059537541496 1101 78-79 0.6145776040578674 1101 80-81 0.6771734030799301 1101 82-83 0.5765637989280492 1101 84-85 0.6067107876520019 1101 86-87 0.2189253784444425 1101 88-89 0.3172280439892745 1101 90-91 -0.042460611983646146 1101 92-93 0.2536258310642836 1101 94-95 -0.013571414478846577 1101 96-97 -0.05008710042319464 1101 98-99 0.6102430171554865 1101 100-101 0.46493950540964235 1102 1 0.1081143184490756 1102 2 0.13425506579621782 1102 3 0.03916248044912152 1102 4 -0.06924760134705821 1102 5 0.0188389270449818 1102 6 0.0038976490337532255 1102 7 -0.16682530106487548 1102 8 -0.08170890420649357 1102 9 0.0757229168226985 1102 10-11 0.08116809250460477 1102 12-13 0.11745174304772377 1102 14-15 0.08361000473123426 1102 16-17 0.0013181871460758998 1102 18-19 0.09372435785764566 1102 20-21 0.11569325438798472 1102 22-23 0.040826086208703316 1102 24-25 0.209725000249243 1102 26-27 0.018967430282188502 1102 28-29 0.01011222109050891 1102 30-31 -0.01892737741918893 1102 32-33 0.01972270029877876 1102 34-35 -0.08900134397220683 1102 36-37 -0.06817113604529368 1102 38-39 -0.035965802755995924 1102 40-41 -0.2221553372394638 1102 42-43 -0.2910841949920666 1102 44-45 -0.6166288078278512 1102 46-47 -0.514177359665382 1102 48-49 -0.5825963680629727 1102 50-51 -0.4383852988046115 1102 52-53 -0.4670356232136683 1102 54-55 -0.2367227443098372 1102 56-57 -0.33103883337528117 1102 58-59 -0.36438871707389353 1102 60-61 -0.39833292579542956 1102 62-63 -0.4556232333083656 1102 64-65 -0.21627712198935 1102 66-67 -0.3675208121372364 1102 68-69 -0.16222792765056226 1102 70-71 -0.16756606493190063 1102 72-73 0.05397125499285238 1102 74-75 -0.17970543286054053 1102 76-77 0.04277172406187546 1102 78-79 -0.06597588340295246 1102 80-81 -0.021141643942012678 1102 82-83 -0.05071872458292148 1102 84-85 -0.4008293769248006 1102 86-87 -0.38438183158690364 1102 88-89 -0.5375564387693466 1102 90-91 -0.8829435665290966 1102 92-93 -0.618897679907505 1102 94-95 -0.7010694552311953 1102 96-97 -0.8942269907053273 1102 98-99 -1.1626155251642665 1102 100-101 -1.2267875713928653 1103 1 0.060790217696222726 1103 2 0.1047541828145313 1103 3 0.13626258268910618 1103 4 0.0682168470217519 1103 5 0.13136983140412184 1103 6 0.002588977224107225 1103 7 0.10226290415865691 1103 8 0.05983027229689952 1103 9 0.0889787956218413 1103 10-11 0.14231503924687416 1103 12-13 0.17962016018685745 1103 14-15 0.20562320298385828 1103 16-17 0.21696183432518268 1103 18-19 0.18196861847666668 1103 20-21 0.17249221339903897 1103 22-23 0.2795099787637696 1103 24-25 0.21287492124561425 1103 26-27 0.16111446996989542 1103 28-29 0.2350290349207924 1103 30-31 0.21747470455868978 1103 32-33 0.14292002347007582 1103 34-35 0.19242350963872212 1103 36-37 0.5134303139035836 1103 38-39 0.37343562860380075 1103 40-41 0.3326601660601014 1103 42-43 0.3354582346017665 1103 44-45 0.3753760253350791 1103 46-47 0.12504747535646032 1103 48-49 0.1698360141286841 1103 50-51 0.07999001488624913 1103 52-53 0.1569284998460958 1103 54-55 0.07070359642815305 1103 56-57 -0.016401785322493367 1103 58-59 0.10043236295212665 1103 60-61 0.28812213508383167 1103 62-63 0.21059946396832885 1103 64-65 0.20249042146264884 1103 66-67 0.317415520235663 1103 68-69 0.3868407590303491 1103 70-71 0.270295260470089 1103 72-73 0.25517582790855187 1103 74-75 0.11879535717570988 1103 76-77 0.17344836687254173 1103 78-79 -0.0617635960156484 1103 80-81 0.007132359218136486 1103 82-83 0.18969860044728648 1103 84-85 0.1235743780710159 1103 86-87 -0.013401257184082027 1103 88-89 -0.14107721207541601 1103 90-91 -0.10989774442667155 1103 92-93 -0.314402838379479 1103 94-95 -0.3710397126900702 1103 96-97 -0.5182589639370399 1103 98-99 -0.5960368468484383 1103 100-101 -1.29729133568323 1104 1 0.10190391220181994 1104 2 0.18841844212332148 1104 3 0.07216239270672986 1104 4 0.14733044550737873 1104 5 0.0618993815505533 1104 6 0.09443550987432303 1104 7 0.05862525609929037 1104 8 0.0993527786925128 1104 9 0.21873950013409882 1104 10-11 0.1658210713586925 1104 12-13 0.08750614332849693 1104 14-15 0.3149796634133395 1104 16-17 0.18927378949757667 1104 18-19 0.10555729635023425 1104 20-21 -0.04802965514961244 1104 22-23 0.13525883166803254 1104 24-25 0.1705146817443719 1104 26-27 0.1512724228240856 1104 28-29 0.08346836744481578 1104 30-31 0.08918001926414121 1104 32-33 0.2317162073620409 1104 34-35 0.3226025868867879 1104 36-37 0.33696618079247287 1104 38-39 0.4022416202500523 1104 40-41 0.3282158130632524 1104 42-43 0.22179287792186386 1104 44-45 0.44025809223356305 1104 46-47 0.5680030386850632 1104 48-49 0.5867341575139307 1104 50-51 0.5832958453561332 1104 52-53 0.5585491798046789 1104 54-55 0.5509011918137006 1104 56-57 0.3948706164799418 1104 58-59 0.4653322621311631 1104 60-61 0.46567163680876433 1104 62-63 0.5153445652351039 1104 64-65 0.47690178474049105 1104 66-67 0.3275164783778166 1104 68-69 0.3418468288643055 1104 70-71 0.17830126857692008 1104 72-73 0.1435851884841206 1104 74-75 0.16326289213726852 1104 76-77 0.33263747820770107 1104 78-79 0.4245312676017008 1104 80-81 0.2720583209610332 1104 82-83 0.1393927082502806 1104 84-85 0.19590485138089875 1104 86-87 0.41181804557374946 1104 88-89 0.1834017081114112 1104 90-91 0.0347409118424018 1104 92-93 -0.09939868894497295 1104 94-95 0.22665889433447717 1104 96-97 0.20538869763370826 1104 98-99 0.28709141525865434 1104 100-101 -0.5729663903803193 1105 1 0.15332619012518478 1105 2 0.13152732518891241 1105 3 0.1699653625901405 1105 4 0.06763351033590936 1105 5 0.07241930103708683 1105 6 0.1169564927462261 1105 7 0.14385749186701702 1105 8 0.13739728896557324 1105 9 0.10089361505566785 1105 10-11 0.18116895028467184 1105 12-13 0.14058521362586873 1105 14-15 0.21323172372248678 1105 16-17 0.0718551574668922 1105 18-19 0.09169056132308384 1105 20-21 0.14118647792546568 1105 22-23 0.21441932690178334 1105 24-25 0.18072602958532258 1105 26-27 0.2696167697915399 1105 28-29 0.21875092412705044 1105 30-31 0.1868025934162887 1105 32-33 0.17125040659488633 1105 34-35 0.3145369987967044 1105 36-37 0.39720886052358395 1105 38-39 0.33843375119836594 1105 40-41 0.4506333057524827 1105 42-43 0.2872860228840679 1105 44-45 0.314878311746682 1105 46-47 0.43783327680742445 1105 48-49 0.5516281987580882 1105 50-51 0.3276466106138116 1105 52-53 0.24777480739192725 1105 54-55 0.2499773757793733 1105 56-57 0.19382906849467219 1105 58-59 0.14419937693880058 1105 60-61 0.15479282476212575 1105 62-63 0.14162157712223689 1105 64-65 0.09333773476137708 1105 66-67 -0.04409398077679327 1105 68-69 -0.10693027796794041 1105 70-71 0.08997382184113079 1105 72-73 0.01085665557614135 1105 74-75 -0.06842219388301274 1105 76-77 -0.2769529285372023 1105 78-79 -0.11233117590595398 1105 80-81 -0.2322284512846089 1105 82-83 -0.19413526258257718 1105 84-85 -0.22432225735025568 1105 86-87 -0.32240207519644315 1105 88-89 -0.3101040455629658 1105 90-91 -0.44115252175297925 1105 92-93 -0.2912068238429981 1105 94-95 -0.19922694365116556 1105 96-97 -0.22730470557923255 1105 98-99 -0.11006877615071176 1105 100-101 -0.9594689918766548 1106 1 0.19495921777473058 1106 2 0.20707538240628764 1106 3 0.1699291359563162 1106 4 0.13422841376605277 1106 5 0.16039136857733638 1106 6 0.1996946742382093 1106 7 0.184512400403392 1106 8 0.18001188588389283 1106 9 0.21447273747408957 1106 10-11 0.19563459018502982 1106 12-13 0.12157054356424624 1106 14-15 0.1130107412561756 1106 16-17 0.2795648751751614 1106 18-19 0.23817985549024456 1106 20-21 0.3165923887320403 1106 22-23 0.3134120539737779 1106 24-25 0.3336257128610356 1106 26-27 0.39590367783109315 1106 28-29 0.15741147021768143 1106 30-31 0.24515507511321033 1106 32-33 0.2864968167079809 1106 34-35 0.3258008942983892 1106 36-37 0.47452339740920024 1106 38-39 0.3569032966748722 1106 40-41 0.2090111460930757 1106 42-43 0.37399878362958106 1106 44-45 0.32011996064505155 1106 46-47 0.33038335581598943 1106 48-49 0.20787280788996298 1106 50-51 0.3426754884162051 1106 52-53 0.25215132977308485 1106 54-55 0.20364264858317682 1106 56-57 0.5366822436728782 1106 58-59 0.398596600253569 1106 60-61 0.08360403914789316 1106 62-63 0.22583642521188807 1106 64-65 0.4196939909285291 1106 66-67 0.33004865555609797 1106 68-69 0.1992279047661043 1106 70-71 -0.1707130351361208 1106 72-73 -0.048555886429500816 1106 74-75 0.062119618552728184 1106 76-77 0.12310555160025416 1106 78-79 0.30883018097124193 1106 80-81 0.219625489828136 1106 82-83 0.3272237882106097 1106 84-85 -0.10205564312595428 1106 86-87 0.06706873993731932 1106 88-89 0.09275682346223135 1106 90-91 -0.09889363587722855 1106 92-93 -0.16994938013228733 1106 94-95 -0.2969353503003056 1106 96-97 -0.4403535390816238 1106 98-99 -0.49202773999997973 1106 100-101 -1.3298914979316798 1107 1 0.001533956752794552 1107 2 -0.052187207947454795 1107 3 -0.012471429558331693 1107 4 -0.0706002356101294 1107 5 -0.03097159431550267 1107 6 -0.08032501778983203 1107 7 -8.498587519056855E-4 1107 8 -0.01797346817024703 1107 9 -0.13871041258768457 1107 10-11 -0.026531880034717403 1107 12-13 -0.12638412059042992 1107 14-15 -0.009530319304701607 1107 16-17 -0.02992264295081526 1107 18-19 -0.01448716777885295 1107 20-21 -0.06366808883120001 1107 22-23 -0.13019505850903812 1107 24-25 0.018259777229353347 1107 26-27 -0.16383669666456768 1107 28-29 -0.19273898129464584 1107 30-31 -0.04370867277833668 1107 32-33 -0.0041673000935205096 1107 34-35 0.042923649162624145 1107 36-37 -0.18180732136561062 1107 38-39 -0.0401829774450988 1107 40-41 -0.1553576439359361 1107 42-43 -0.1067336398941876 1107 44-45 -0.3006341430442063 1107 46-47 -0.20898807717352952 1107 48-49 -0.24213973631059105 1107 50-51 -0.3286877584962724 1107 52-53 -0.352190383717371 1107 54-55 -0.1535404446746682 1107 56-57 -0.3030360088489772 1107 58-59 -0.26379478195953965 1107 60-61 -0.21869383748975224 1107 62-63 -0.15675853596420808 1107 64-65 -0.07248205706447663 1107 66-67 -0.24520234679653186 1107 68-69 -0.22817983224433647 1107 70-71 -0.4657968130390735 1107 72-73 -0.31320774936162366 1107 74-75 -0.08437060880499203 1107 76-77 -0.14914699234784123 1107 78-79 -0.09988275249400047 1107 80-81 0.04446190240767933 1107 82-83 0.0623447095703078 1107 84-85 -0.23275280087834815 1107 86-87 -0.5764645274403435 1107 88-89 -0.2961465293895671 1107 90-91 -0.22173765071567786 1107 92-93 -0.3349174515916289 1107 94-95 -0.2416343604918758 1107 96-97 -0.5817653375728504 1107 98-99 -0.6330490114927727 1107 100-101 -0.8371284760258746 1108 1 -0.0936390937092284 1108 2 -0.03818914208881097 1108 3 -0.09905749445868395 1108 4 -0.026394694834493748 1108 5 -0.011148526857617469 1108 6 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0.21427835865384282 2316 76-77 -0.07230852383261421 2316 78-79 0.11083009148978817 2316 80-81 -0.04775851983586321 2316 82-83 -0.08407324547915351 2316 84-85 -0.02426494024912884 2316 86-87 0.04499366499659985 2316 88-89 0.022786799749646036 2316 90-91 0.12581219383956466 2316 92-93 0.4393924837664187 2316 94-95 0.808366425906577 2316 96-97 1.024346026933319 2316 98-99 1.1881297473607653 2316 100-101 1.6141468001310493 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 8.0 5 18.0 6 61.0 7 124.0 8 253.0 9 423.0 10 847.0 11 1445.0 12 2469.0 13 3748.0 14 4806.0 15 6385.0 16 8634.0 17 12323.0 18 11726.0 19 7222.0 20 5656.0 21 5834.0 22 6441.0 23 7040.0 24 7600.0 25 8730.0 26 9437.0 27 10406.0 28 12375.0 29 14867.0 30 18102.0 31 22177.0 32 27522.0 33 36120.0 34 52733.0 35 87894.0 36 113398.0 37 185836.0 38 137790.0 39 8251.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.24790226091975 24.806085082890057 16.2043114991993 19.741701156990892 2 13.801677136846926 24.44739390296838 41.485406142823756 20.265522817360942 3 15.688909504350512 31.984517735325298 32.792018477601154 19.534554282723036 4 9.871897594383716 21.524161086628894 42.2441051908659 26.359836128121483 5 9.046803721023402 42.32962200754983 36.80567644456739 11.81789782685938 6 23.240959775651724 44.24094069841754 18.974813281570437 13.543286244360292 7 22.827839142295733 37.86210629733493 24.330395467292394 14.979659093076938 8 23.356869646502222 36.860680287681944 22.024218317785536 17.758231748030294 9 25.622269596901887 17.808070546939085 23.986054674831646 32.58360518132738 10-11 21.225605219481487 30.489481390335566 29.65497958755413 18.629933802628816 12-13 22.75422470808886 30.21490478683005 29.52222316283473 17.508647342246366 14-15 20.88048942177455 31.04951210438963 28.53766048570175 19.532337988134074 16-17 16.547790233770446 34.364648986629085 30.127663988667987 18.959896790932483 18-19 16.29627377477632 32.019997519983214 34.643032583605184 17.040696121635285 20-21 17.02364600621912 31.465332226864305 34.93187107728114 16.579150689635437 22-23 17.52787634254755 31.119918350216523 34.49297964478529 16.85922566245064 24-25 17.001074915595577 32.35179966387276 34.56578000537756 16.081345415154104 26-27 16.350707933204735 32.15242104509962 35.207699164910125 16.289171856785515 28-29 16.125078102269853 31.15547240545452 35.96371046594264 16.755739026332986 30-31 16.651295225274584 31.603544394054207 35.255786945264354 16.489373435406858 32-33 15.991219786718554 32.3976635380301 35.150064861977526 16.461051813273812 34-35 16.499111646375635 31.745286956106984 35.096407235610464 16.65919416190692 36-37 17.08857061779091 31.029762799005123 34.509203551218675 17.37246303198529 38-39 16.032980496078647 32.45925877348564 34.62659961665837 16.881161113777345 40-41 15.82480160871175 31.620390767196444 36.02962261687514 16.525185007216667 42-43 16.13068232628849 31.45485438340239 34.90553551733389 17.508927772975227 44-45 16.066216448234417 31.592611934603866 34.69424254564185 17.646929071519867 46-47 16.898771078677434 31.358120812731087 34.068078926628374 17.675029181963104 48-49 16.438425580648065 31.65602813413434 33.832500791406666 18.073045493810934 50-51 16.33575443874293 31.37027650442697 33.87443042019682 18.419538636633273 52-53 16.326589016398135 31.994068912228485 34.098269562811815 17.58107250856156 54-55 16.91439064670566 31.51579650265014 33.50867779188801 18.061135058756193 56-57 16.977413930858486 32.046460045076735 32.78623132237023 18.189894701694552 58-59 16.229745539457067 31.23292376201374 33.8495013231662 18.68782937536299 60-61 16.998568577991634 32.54914141507482 32.4300251646493 18.02226484228424 62-63 16.61260509212331 32.269214715878604 33.22544869119313 17.892731500804963 64-65 16.690358610968335 31.54133190863655 33.08496543400979 18.68334404638533 66-67 16.628696569335624 32.27587456929627 32.09581223911085 18.999616622257253 68-69 16.499871787991008 31.566094781473065 32.84111848578636 19.09291494474957 70-71 17.039275861184862 31.446506755262092 32.81789144238068 18.69632594117237 72-73 17.19148048231559 30.921198932824588 32.397418508637266 19.489902076222556 74-75 16.705194488484285 31.761155727329154 31.710355050225292 19.823294733961266 76-77 16.574501629037414 31.133695553658058 32.880259787342645 19.411543029961887 78-79 16.506210819007595 30.720044746758035 32.57634349099847 20.1974009432359 80-81 16.8708230907325 31.275074870604875 31.653199000493075 20.200903038169553 82-83 17.236688620711867 30.820247179807687 31.782297135796227 20.160767063684222 84-85 16.533983205022633 30.493145996330455 31.919162767456406 21.053708031190503 86-87 16.63217867127196 30.83095594759892 32.26615350119744 20.27071187993168 88-89 17.23857061677615 30.128397390908727 32.12272197936984 20.510310012945283 90-91 16.91370841699081 29.7753040143067 32.522509253257496 20.788478315444994 92-93 17.34502942109494 30.233961508096467 31.920755050005127 20.500254020803467 94-95 16.860789924153305 29.06333714723178 32.50842625114849 21.567446677466418 96-97 17.302968294625778 29.069718911132412 31.860991466689565 21.766321327552244 98-99 16.62815142333499 29.325422803170415 31.90403623056202 22.142389542932573 100-101 16.844594900755276 28.78442801031201 32.571061988901 21.79991510003172 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7126.0 1 4144.0 2 1656.0 3 2089.0 4 1533.5 5 1517.0 6 3289.0 7 6190.0 8 8353.0 9 8254.0 10 5945.0 11 3910.0 12 3396.0 13 3151.5 14 2877.5 15 2482.0 16 2188.0 17 2123.0 18 2142.5 19 2256.5 20 2502.5 21 2763.5 22 3019.0 23 3332.5 24 3550.5 25 4437.5 26 7001.0 27 9368.5 28 9482.0 29 10504.0 30 14477.5 31 18329.0 32 23664.0 33 30805.5 34 37117.0 35 42349.5 36 49270.5 37 54807.0 38 52965.0 39 48861.0 40 44098.0 41 38491.5 42 33106.5 43 31843.5 44 31939.5 45 29397.5 46 24933.0 47 20522.5 48 20841.0 49 18282.0 50 12575.5 51 8674.5 52 5708.0 53 3865.5 54 2754.0 55 2142.5 56 1772.5 57 1493.0 58 1202.0 59 1020.5 60 925.0 61 800.0 62 636.0 63 448.0 64 294.5 65 217.5 66 182.5 67 149.5 68 117.0 69 107.0 70 102.0 71 88.0 72 68.0 73 57.0 74 46.5 75 39.0 76 32.0 77 22.5 78 16.5 79 9.0 80 6.0 81 4.0 82 2.5 83 1.5 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0075115893092199395 2 0.015738568076460825 3 0.007988515614567237 4 0.0 5 0.0011923157633682443 6 9.538526106945954E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.346210343577711E-4 14-15 0.0 16-17 0.00214616837406284 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003994257807283619 26-27 0.02044821534176539 28-29 0.0071538945802094665 30-31 0.003278868349262672 32-33 0.0 34-35 0.008823136648925008 36-37 7.153894580209466E-4 38-39 0.0031596367729258474 40-41 0.002444247314904901 42-43 0.0034577157137679086 44-45 0.0 46-47 1.1923157633682443E-4 48-49 8.942368225261833E-4 50-51 0.004471184112630916 52-53 0.00858467349625136 54-55 0.008226978767240887 56-57 0.015977031229134474 58-59 0.02205784162231252 60-61 0.003278868349262672 62-63 0.018361662755870964 64-65 0.019434746942902384 66-67 0.012578931303534976 68-69 0.029688662507869286 70-71 0.016155878593639712 72-73 0.004948110417978214 74-75 0.015798183864629237 76-77 0.020507831129933804 78-79 0.024740552089891073 80-81 0.01079045765848261 82-83 0.03410023083233179 84-85 0.021402067952459986 86-87 0.02956943093153246 88-89 0.020805910070775865 90-91 0.025694404700585666 92-93 0.022653999503996644 94-95 0.012459699727198154 96-97 0.01287701024437704 98-99 0.008942368225261833 100-101 0.008346210343577711 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 838704.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.564929400198263 #Duplication Level Percentage of deduplicated Percentage of total 1 73.28490612391985 12.872442021668096 2 10.045908502127071 3.5291134720142696 3 3.90653826130868 2.0585420627718074 4 2.137781377149569 1.5019991585076318 5 1.382363384769986 1.2140557629451956 6 0.9968975427566149 1.050626097465064 7 0.70763939461177 0.8700745205008344 8 0.5471099718350989 0.7687958423542378 9 0.460578471528709 0.7281025502087699 >10 4.5887393319661465 17.668808364085802 >50 0.9643173997893172 12.067489757828803 >100 0.9011614010707211 30.43836331435387 >500 0.06247690195818112 7.479155014042363 >1k 0.010865548166640194 3.0733230560405973 >5k 0.0013581935208300242 1.851508654215492 >10k+ 0.0013581935208300242 2.8276003509971805 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13382 1.5955569545393848 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10323 1.2308275625250387 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7818 0.9321524638012934 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7704 0.9185600640988955 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3213 0.38309105477021693 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2293 0.2733980045403384 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2175 0.2593286785325931 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 2023 0.2412054789293958 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1890 0.22534767927659816 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1706 0.20340906923062252 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1432 0.1707396173143326 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1379 0.1644203437684809 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1372 0.1635857227341231 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1318 0.15714721761193462 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1314 0.1566702913065873 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 1290 0.15380873347450352 RNA PCR Primer, Index 27 (95% over 23bp) GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1124 0.13401629180259067 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1109 0.1322278181575383 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1083 0.12912779717278086 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1044 0.1244777656956447 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 998 0.11899311318415079 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 996 0.11875465003147714 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 966 0.1151777027413724 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 957 0.11410461855434098 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 954 0.1137469238253305 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 948 0.11303153436730956 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 947 0.11291230279097274 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 946 0.11279307121463591 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 932 0.11112382914592037 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 928 0.11064690284057307 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 888 0.10587763978710009 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 883 0.10528148190541597 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 877 0.10456609244739502 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 870 0.10373147141303726 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 865 0.10313531353135312 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 840 0.10015452412293253 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.1923157633682444E-4 0.0 14-15 0.0 0.0 0.0 1.1923157633682444E-4 0.0 16-17 0.0 0.0 0.0 1.1923157633682444E-4 0.0 18-19 0.0 0.0 0.0 2.9807894084206107E-4 0.0 20-21 0.0 0.0 0.0 6.557736698525345E-4 0.0 22-23 0.0 0.0 0.0 0.002086552585894428 0.0 24-25 0.0 0.0 0.0 0.0059019630286728094 0.0 26-27 0.0 0.0 0.0 0.014367404948587344 0.0 28-29 0.0 0.0 0.0 0.030702130906732295 0.0 30-31 0.0 0.0 0.0 0.07654667200824129 0.0 32-33 0.0 0.0 0.0 0.1532721913809878 0.0 34-35 0.0 0.0 0.0 0.26850950991052863 0.0 36-37 0.0 0.0 0.0 0.4415741429634293 0.0 38-39 0.0 0.0 0.0 0.6783680535683626 0.0 40-41 0.0 0.0 0.0 0.9259524218317785 0.0 42-43 0.0 0.0 0.0 1.1709733112039529 0.0 44-45 0.0 0.0 0.0 1.4419270684293863 0.0 46-47 0.0 0.0 0.0 1.7674888876170853 0.0 48-49 0.0 0.0 0.0 2.098714206680784 0.0 50-51 0.0 0.0 0.0 2.4325030046357234 0.0 52-53 0.0 0.0 0.0 2.7454858925198877 0.0 54-55 0.0 0.0 0.0 3.07844006944047 0.0 56-57 0.0 0.0 0.0 3.4531849138671094 0.0 58-59 0.0 0.0 0.0 3.850166447280566 0.0 60-61 0.0 0.0 0.0 4.264019248745684 0.0 62-63 0.0 0.0 0.0 4.644844903565501 0.0 64-65 0.0 0.0 0.0 5.045701463209904 0.0 66-67 0.0 0.0 0.0 5.47165626967321 0.0 68-69 0.0 0.0 0.0 5.909057307464851 0.0 70-71 0.0 0.0 0.0 6.398681775692021 0.0 72-73 0.0 0.0 0.0 6.865890707567866 0.0 74-75 0.0 0.0 0.0 7.341267002422786 0.0 76-77 0.0 0.0 0.0 7.840310765180565 0.0 78-79 0.0 0.0 0.0 8.39092218470402 0.0 80-81 0.0 0.0 0.0 8.973964592991091 0.0 82-83 0.0 0.0 0.0 9.529881817661535 0.0 84-85 0.0 0.0 0.0 10.085262500238464 0.0 86-87 0.0 0.0 0.0 10.656143287739178 0.0 88-89 0.0 0.0 0.0 11.246995364276312 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTAGAG 35 2.7874988E-4 54.285748 1 CCTTATG 45 9.629202E-4 42.217213 2 TAGGCAT 210 0.0 38.440945 5 CATTCCG 210 0.0 38.438652 9 GTGTACG 50 0.0016184038 37.98643 9 GGTATCA 5645 0.0 37.108086 1 GGCATTC 220 0.0 36.69144 7 GCACTGA 65 1.330672E-4 36.525414 6 GTACAGG 80 1.0772559E-5 35.62502 1 GTATCAA 8770 0.0 33.634567 1 CTTTAGG 255 0.0 33.525433 2 TACTCCC 170 3.6379788E-12 33.519436 5 GTGGTAC 190 0.0 32.50002 1 CCTTTAG 235 0.0 32.340446 1 TGGTACT 195 0.0 31.662912 2 GTCATGC 60 0.00395726 31.65536 8 AGGGTCT 140 1.7096681E-8 30.52663 5 TCGTATC 55 2.7363312E-6 30.224586 94-95 GGTACTC 205 1.8189894E-12 30.114786 3 GTTATCT 80 4.4925997E-4 29.687517 1 >>END_MODULE