FastQCFastQC Report
Thu 26 May 2016
SRR938211_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938211_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252812
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60280.48115758789028207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49460.394791876195311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32000.2554253950313375No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA21480.1714542964147853No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20180.16107763974163722No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA17820.14224001685807608No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA17420.13904719942018437No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA15330.12236472830720012No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG13970.11150914901836828No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13600.10855579288831843No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA13570.10831633158047656No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC13560.10823651114452927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24350.056.381821
GTATCAA42100.045.2922021
GTGGTAT9250.036.0746271
TATCAAC53850.035.2151572
TGGTATC8800.035.1053352
ATCAACG53850.034.9503823
TCAACGC54650.034.438764
CAACGCA56200.033.4889345
AACGCAG57300.033.0053336
CGCAGAG66050.028.1967858
ACGCAGA66550.028.056337
GCAGAGT72700.025.2898089
TAAGGTG7000.025.1215325
CAGAGTA68350.023.21497710-11
TACATGG38150.023.0472772
GTACATG40550.023.0415021
GAGTACT50750.022.65327512-13
AGAGTAC65900.021.98744410-11
ACATGGG38850.021.7756633
GTACTTT52900.021.73128114-15
Produced by FastQC (version 0.11.5)