FastQCFastQC Report
Thu 26 May 2016
SRR938211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938211_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252812
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61160.48818178625364383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54330.43366442850164266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35180.28080829366257665No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA18460.14734852475870283No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16430.13114497626140234No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC16070.1282714405672998No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA15220.1214867035117799No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA15010.11981047435688674No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14870.11869298825362465No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG14350.11454232558436542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26600.063.0987051
TGGTATC8700.043.7218062
GTATCAA46350.042.059251
GTGGTAT9850.038.6172331
TATCAAC62050.031.3407152
ATCAACG62050.031.336963
TCAACGC63150.030.7911074
CAACGCA64500.030.146645
AACGCAG66650.029.1450586
TAAGGTG8450.027.0059765
ACGCAGA77450.024.9582867
ATACCGT1151.2828821E-424.7796526
CGCAGAG77600.024.7264298
GTAAGGT8250.024.2030814
AGGTAAG7700.023.4649852
GAGTACT60300.022.56571612-13
GCAGAGT84750.022.3041239
GTGTAGG4350.021.8609031
CAGAGTA80500.021.62319610-11
GTACTTT63100.021.4514814-15
Produced by FastQC (version 0.11.5)