##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938211_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1252812 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.65312273509513 31.0 31.0 34.0 30.0 34.0 2 31.968278560550186 31.0 31.0 34.0 30.0 34.0 3 32.03097272376063 33.0 31.0 34.0 30.0 34.0 4 35.71949422578967 37.0 35.0 37.0 33.0 37.0 5 35.59258851288142 37.0 35.0 37.0 33.0 37.0 6 35.670710369951756 37.0 35.0 37.0 33.0 37.0 7 35.617494085305694 37.0 35.0 37.0 33.0 37.0 8 35.59651168730823 37.0 35.0 37.0 33.0 37.0 9 37.108224538079135 39.0 37.0 39.0 33.0 39.0 10-11 37.012095190659096 39.0 37.0 39.0 33.0 39.0 12-13 36.85647846604279 39.0 37.0 39.0 33.0 39.0 14-15 37.72466140171071 39.0 37.0 40.5 32.5 41.0 16-17 37.520154660076685 39.0 36.0 40.0 32.0 41.0 18-19 37.859717579333534 39.5 37.0 41.0 33.0 41.0 20-21 37.787100937730486 39.0 37.0 41.0 32.5 41.0 22-23 37.57851297720647 39.0 36.5 40.5 32.0 41.0 24-25 37.343893577009155 39.0 36.0 40.0 32.0 41.0 26-27 37.02286336657056 39.0 36.0 40.0 31.0 41.0 28-29 36.78858080861295 39.0 36.0 40.0 30.5 41.0 30-31 36.36756392818715 38.0 35.0 40.0 30.0 41.0 32-33 35.97669283180557 38.0 35.0 40.0 30.0 41.0 34-35 35.7590205074664 38.0 34.0 40.0 29.0 41.0 36-37 35.59370919180212 38.0 34.0 40.0 28.5 41.0 38-39 35.23054815886182 38.0 34.0 40.0 27.0 41.0 40-41 34.791340999288 38.0 33.0 40.0 25.5 41.0 42-43 34.317842581329046 37.5 33.0 40.0 24.5 41.0 44-45 33.820610354945515 37.0 32.5 40.0 23.5 41.0 46-47 33.19895283570081 37.0 31.5 39.0 21.5 40.0 48-49 32.46214036902584 36.0 31.0 39.0 17.5 40.0 50-51 31.725940923298946 35.5 30.0 39.0 12.0 40.0 52-53 30.745726014757203 35.0 28.5 38.0 8.0 40.0 54-55 29.918382007835174 34.0 27.5 38.0 4.5 40.0 56-57 29.145404897143386 34.0 26.0 38.0 2.0 40.0 58-59 28.20159648853938 33.0 24.5 37.5 2.0 39.5 60-61 27.28086736078518 32.5 22.5 37.0 2.0 39.0 62-63 26.26686486080912 31.5 20.0 36.0 2.0 39.0 64-65 25.25631499378997 31.0 15.5 35.5 2.0 38.5 66-67 24.286877440509826 30.0 8.5 35.0 2.0 38.0 68-69 23.27509873787927 29.0 4.5 34.5 2.0 38.0 70-71 22.314032751921275 28.5 2.0 34.0 2.0 37.0 72-73 21.336103501562885 27.5 2.0 34.0 2.0 36.0 74-75 20.467993202491677 26.0 2.0 33.0 2.0 36.0 76-77 19.7193182217284 25.5 2.0 31.5 2.0 34.5 78-79 20.104091435905787 26.0 2.0 33.0 2.0 35.0 80-81 19.635032630594218 26.0 2.0 33.0 2.0 35.0 82-83 18.847744513941436 25.0 2.0 33.0 2.0 35.0 84-85 17.928722346209966 23.0 2.0 32.0 2.0 35.0 86-87 16.915485324214647 19.0 2.0 31.5 2.0 34.0 88-89 15.833037598618148 9.5 2.0 31.0 2.0 34.0 90-91 14.923485726509643 2.0 2.0 31.0 2.0 34.0 92-93 13.784111662404255 2.0 2.0 30.0 2.0 34.0 94-95 12.691389450292622 2.0 2.0 29.0 2.0 34.0 96-97 11.546941201074064 2.0 2.0 28.0 2.0 33.5 98-99 10.277871699824075 2.0 2.0 25.0 2.0 33.0 100-101 8.277105024536802 2.0 2.0 9.5 2.0 30.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.325267089788575 1101 2 0.29694422370486606 1101 3 0.3939614045319786 1101 4 0.2907094565373356 1101 5 0.12322849415534165 1101 6 0.21710832355690002 1101 7 0.22878952158124122 1101 8 0.2739579832195531 1101 9 0.32233559663455935 1101 10-11 0.5174702864524434 1101 12-13 0.42619282003781933 1101 14-15 0.5663169403155379 1101 16-17 0.6921274854811585 1101 18-19 0.5876002425078255 1101 20-21 0.5603962068921149 1101 22-23 0.5337406490754617 1101 24-25 0.6507713510944697 1101 26-27 0.9267380701166061 1101 28-29 0.9790919148730808 1101 30-31 1.2118027927538364 1101 32-33 0.9736957992763351 1101 34-35 1.0607972242709565 1101 36-37 1.2166660053512501 1101 38-39 1.395876462453451 1101 40-41 1.6502179284270753 1101 42-43 1.7856747301175346 1101 44-45 2.014188164743068 1101 46-47 1.9556028529956677 1101 48-49 2.2410956496540777 1101 50-51 2.523331175110375 1101 52-53 2.4901681887904914 1101 54-55 2.9769126146675013 1101 56-57 3.1749215836951343 1101 58-59 3.2067485964498097 1101 60-61 3.232666841315993 1101 62-63 3.5970173688704925 1101 64-65 3.590037683704928 1101 66-67 3.425944224833035 1101 68-69 4.0419146103504175 1101 70-71 4.508293605414341 1101 72-73 4.703013966261651 1101 74-75 4.6119808236406215 1101 76-77 4.7263288082622275 1101 78-79 4.769667383277934 1101 80-81 5.125173801016377 1101 82-83 4.988281044554622 1101 84-85 5.177383077197202 1101 86-87 5.418359041036219 1101 88-89 5.3830060163435505 1101 90-91 5.490161930322261 1101 92-93 5.074300423981592 1101 94-95 4.978722134715442 1101 96-97 4.264768877172058 1101 98-99 3.9260976376975396 1101 100-101 3.3631083809332196 1102 1 0.17888909414587317 1102 2 0.1974024709768365 1102 3 0.2522142174416544 1102 4 0.15245129131838553 1102 5 -0.027450211670419833 1102 6 0.11060326362046169 1102 7 0.037090788215842 1102 8 0.211166199839532 1102 9 0.31120715535905674 1102 10-11 0.24187764198724437 1102 12-13 0.3257453599089857 1102 14-15 0.4861944030851575 1102 16-17 0.49207991594829537 1102 18-19 0.4297796226774011 1102 20-21 0.40964116581844934 1102 22-23 0.4591505015686437 1102 24-25 0.511261169917617 1102 26-27 0.43233205855373313 1102 28-29 0.4189966225474038 1102 30-31 0.48444341465916096 1102 32-33 0.5011464259916707 1102 34-35 0.6106624144547794 1102 36-37 0.6463291163869016 1102 38-39 0.938146442836171 1102 40-41 0.806760595999144 1102 42-43 1.1452143372061627 1102 44-45 1.3112563607083203 1102 46-47 1.3409505526816048 1102 48-49 1.5015759781491127 1102 50-51 1.5031957640435927 1102 52-53 1.9321307061206596 1102 54-55 2.1326362256607148 1102 56-57 2.1067780805026075 1102 58-59 2.223147411868233 1102 60-61 2.3282757077581913 1102 62-63 2.4893009658109513 1102 64-65 2.7706262490974183 1102 66-67 3.0569720568412606 1102 68-69 3.3352126113690055 1102 70-71 3.1788908005213408 1102 72-73 3.3468852774123725 1102 74-75 3.526526608284449 1102 76-77 3.44597449481968 1102 78-79 3.637756538131793 1102 80-81 3.8702411882406267 1102 82-83 3.6051839083937587 1102 84-85 3.9221969960307312 1102 86-87 3.946641987211322 1102 88-89 3.6655204109903003 1102 90-91 3.6473303111426016 1102 92-93 3.6589326354520377 1102 94-95 3.4700213901078367 1102 96-97 3.183810778444352 1102 98-99 3.2939666287282954 1102 100-101 2.654408107894712 1103 1 0.34099838230558177 1103 2 0.4285737273581738 1103 3 0.3618216967970369 1103 4 0.3821997563609685 1103 5 0.333971124540831 1103 6 0.37454722504341476 1103 7 0.3476796702332905 1103 8 0.38639184565341367 1103 9 0.3959845006406084 1103 10-11 0.556759150146064 1103 12-13 0.6384077986218486 1103 14-15 0.779831049662981 1103 16-17 0.875871816546919 1103 18-19 0.6721775919661255 1103 20-21 0.6937972524955427 1103 22-23 0.6955965951575962 1103 24-25 0.8630099502378314 1103 26-27 0.941256840587755 1103 28-29 0.9000496498189108 1103 30-31 1.0811810996321398 1103 32-33 1.2242154515816992 1103 34-35 1.2061187788187056 1103 36-37 1.290425138634184 1103 38-39 1.4223866035469186 1103 40-41 1.6524382610045478 1103 42-43 1.7862101294624608 1103 44-45 1.946468021130066 1103 46-47 2.0449594289593875 1103 48-49 2.3418373352171926 1103 50-51 2.358632888388282 1103 52-53 2.7981094208388697 1103 54-55 2.9979742800744056 1103 56-57 3.2024001677213327 1103 58-59 3.3980436329829438 1103 60-61 3.3606745258712927 1103 62-63 3.675421878822622 1103 64-65 3.582510598796894 1103 66-67 3.910008220801796 1103 68-69 4.009530823502342 1103 70-71 4.281851176590958 1103 72-73 4.31504627918682 1103 74-75 4.398293496858059 1103 76-77 4.644082020658299 1103 78-79 4.816436090760927 1103 80-81 4.7607873056775 1103 82-83 4.952999802428142 1103 84-85 5.2113651885602685 1103 86-87 5.459820996188647 1103 88-89 5.346370528815207 1103 90-91 5.284324502758645 1103 92-93 4.762343696846292 1103 94-95 4.915694608890297 1103 96-97 4.8712109910546495 1103 98-99 4.961977267327169 1103 100-101 3.9784003310465987 1104 1 0.328013045278432 1104 2 0.29437962103863313 1104 3 0.3427258101053283 1104 4 0.2161255415062655 1104 5 0.21450200158739108 1104 6 0.26511207379642343 1104 7 0.3870010405651172 1104 8 0.3263723709997919 1104 9 0.34937940873934537 1104 10-11 0.4627034445921083 1104 12-13 0.4415043020667824 1104 14-15 0.586404331846019 1104 16-17 0.7432304870414796 1104 18-19 0.6862715764433887 1104 20-21 0.8094560806979203 1104 22-23 0.767671249113782 1104 24-25 0.7421765959549944 1104 26-27 0.9951833631553981 1104 28-29 0.8952957896643525 1104 30-31 0.7255633659656198 1104 32-33 0.7427943046553267 1104 34-35 0.8192309860075682 1104 36-37 0.958973908228252 1104 38-39 1.0831463496725249 1104 40-41 1.5084599009983108 1104 42-43 1.3955916499938041 1104 44-45 1.2014443649565791 1104 46-47 1.3621608106207077 1104 48-49 1.682776174413867 1104 50-51 1.8309175401154327 1104 52-53 1.9043275671266997 1104 54-55 2.243556868862566 1104 56-57 2.267656178970377 1104 58-59 2.6174066676769847 1104 60-61 2.7573222709510965 1104 62-63 2.8816814213190973 1104 64-65 2.8467751121903255 1104 66-67 3.109603308898624 1104 68-69 3.194330537918784 1104 70-71 3.5287762092302764 1104 72-73 3.7748014549698716 1104 74-75 3.6715637805813017 1104 76-77 3.8710740246537902 1104 78-79 3.9839340335201925 1104 80-81 4.119130988062995 1104 82-83 4.379603654119098 1104 84-85 4.121598264298566 1104 86-87 4.164949265776848 1104 88-89 4.223204238401529 1104 90-91 4.307593529511992 1104 92-93 4.201946293243315 1104 94-95 3.9653507857241745 1104 96-97 3.8249754483034266 1104 98-99 3.4202592743896627 1104 100-101 2.293846144750253 1105 1 0.26805227225644046 1105 2 0.2609878864676496 1105 3 0.31282513559001046 1105 4 0.18880439055180886 1105 5 0.05014454263228885 1105 6 0.151200920678356 1105 7 0.30336743160179935 1105 8 0.15972875031827272 1105 9 0.3365013122401166 1105 10-11 0.44425499791861967 1105 12-13 0.5138928259253461 1105 14-15 0.6114951736968735 1105 16-17 0.5606713090185664 1105 18-19 0.5925191532258296 1105 20-21 0.5491275479788627 1105 22-23 0.5397071422866944 1105 24-25 0.5968201450583948 1105 26-27 0.5956589780629429 1105 28-29 0.8181890748234437 1105 30-31 0.9130218493012094 1105 32-33 1.0744352492912839 1105 34-35 0.9770073249928615 1105 36-37 1.2361786091754183 1105 38-39 1.0917511782673301 1105 40-41 1.190048707481985 1105 42-43 1.1606226481994781 1105 44-45 1.5011102252848971 1105 46-47 1.4305534317842898 1105 48-49 1.4124318565506329 1105 50-51 2.0246892677346686 1105 52-53 1.9724436364685474 1105 54-55 2.1981682078126106 1105 56-57 2.1110980236469423 1105 58-59 2.70904699413828 1105 60-61 2.813635542347665 1105 62-63 2.9179693583997626 1105 64-65 2.8955943714760934 1105 66-67 2.775796583917483 1105 68-69 3.4999040331921876 1105 70-71 3.973917275060998 1105 72-73 3.9944776832802127 1105 74-75 3.7324678122188324 1105 76-77 3.8418029122267967 1105 78-79 4.015139653053211 1105 80-81 4.351605600876887 1105 82-83 4.1534840394913495 1105 84-85 4.417572428935831 1105 86-87 4.50913725811365 1105 88-89 3.9933316528751703 1105 90-91 3.6270567044678916 1105 92-93 3.9479952624370664 1105 94-95 3.958061747316547 1105 96-97 3.7296784018226568 1105 98-99 3.0999626481592113 1105 100-101 2.436016555982812 1106 1 0.16776625936173417 1106 2 0.19106826179377734 1106 3 0.07862411283070969 1106 4 0.06131096237800904 1106 5 -0.06798766595095174 1106 6 0.04754208317601183 1106 7 0.1201766112119742 1106 8 0.13855688705611868 1106 9 0.18034298743342703 1106 10-11 0.23360617196426148 1106 12-13 0.19505561968350804 1106 14-15 0.10673450661128214 1106 16-17 0.14718582296662674 1106 18-19 0.2314336312029468 1106 20-21 0.19192370786094415 1106 22-23 0.16664181444112103 1106 24-25 0.189808332769708 1106 26-27 0.08754330734187477 1106 28-29 0.1665175224687161 1106 30-31 0.32981682696741643 1106 32-33 0.7801336833487511 1106 34-35 0.6097214755027593 1106 36-37 0.6945904918706489 1106 38-39 0.7327408399101287 1106 40-41 0.8176838809266584 1106 42-43 0.792186660583134 1106 44-45 0.7553181680647683 1106 46-47 1.0212948061596023 1106 48-49 1.270570612284871 1106 50-51 1.6144255392802158 1106 52-53 1.549310150258691 1106 54-55 1.5748701106537695 1106 56-57 1.6088991898513605 1106 58-59 2.1236782316156173 1106 60-61 2.140070788611567 1106 62-63 2.1950665578202795 1106 64-65 2.2514380601650963 1106 66-67 2.754945498154189 1106 68-69 2.791307360724108 1106 70-71 2.888200043990434 1106 72-73 3.2741452152236157 1106 74-75 3.16015250054922 1106 76-77 3.3713317147561703 1106 78-79 3.84749913880027 1106 80-81 3.551085886494981 1106 82-83 3.5199635585334725 1106 84-85 3.537615319903285 1106 86-87 3.7991478081291703 1106 88-89 3.4971014739087423 1106 90-91 3.2007979680790033 1106 92-93 2.9307974759663136 1106 94-95 2.8542913231116103 1106 96-97 2.7489924972794597 1106 98-99 2.519733454007122 1106 100-101 1.8875177398437 1107 1 0.2599773962343086 1107 2 0.24369180083030884 1107 3 0.21026160266097094 1107 4 0.1022450963911723 1107 5 0.09312538764232414 1107 6 0.23304158586285695 1107 7 0.20681659609456204 1107 8 0.2841564860372827 1107 9 0.4716875886720189 1107 10-11 0.3252051440705941 1107 12-13 0.3904598011875322 1107 14-15 0.5572323289567649 1107 16-17 0.5141995338732315 1107 18-19 0.37923945616003607 1107 20-21 0.3712645048519434 1107 22-23 0.4120983863863259 1107 24-25 0.5158228459403134 1107 26-27 0.5626818930715345 1107 28-29 0.5834931003760246 1107 30-31 0.7325134636821389 1107 32-33 0.7630932114500553 1107 34-35 0.7260763454941213 1107 36-37 0.9400821416490359 1107 38-39 0.668588574957063 1107 40-41 0.8903541187430477 1107 42-43 1.0383931626555523 1107 44-45 1.3908292124902815 1107 46-47 1.5199191327500685 1107 48-49 1.7082370198281893 1107 50-51 1.569483197054094 1107 52-53 2.1072836246275415 1107 54-55 1.9269536773678482 1107 56-57 2.406448041391787 1107 58-59 2.4064313096954812 1107 60-61 2.5947060979584506 1107 62-63 2.62522913791938 1107 64-65 2.792929479108711 1107 66-67 3.4551986243246304 1107 68-69 3.5578510852431755 1107 70-71 3.87152619202568 1107 72-73 3.7594798520944934 1107 74-75 3.625432800724951 1107 76-77 3.80572117375414 1107 78-79 4.084913268830405 1107 80-81 4.034527451916837 1107 82-83 4.4071156232034205 1107 84-85 3.952846624266858 1107 86-87 4.105895255651035 1107 88-89 4.214356599515671 1107 90-91 3.9127634130186824 1107 92-93 4.038231543784212 1107 94-95 3.8029676441032034 1107 96-97 3.6771689365172975 1107 98-99 3.5185982334863635 1107 100-101 2.5282969656193437 1108 1 0.3228972912489425 1108 2 0.3241630002803326 1108 3 0.18104059575804143 1108 4 0.24870555635845903 1108 5 0.11487646635557525 1108 6 0.1885864327032536 1108 7 0.2697670588968606 1108 8 0.23707490061200076 1108 9 0.18625637608383983 1108 10-11 0.17316036885471675 1108 12-13 0.34214561467727833 1108 14-15 0.46345155513621705 1108 16-17 0.45301151380877513 1108 18-19 0.32804590392537847 1108 20-21 0.40284492540477146 1108 22-23 0.46635351233459943 1108 24-25 0.4928397655104604 1108 26-27 0.611149817572695 1108 28-29 0.5626497650827531 1108 30-31 0.6404064260783642 1108 32-33 0.645217528844384 1108 34-35 0.7112006215320363 1108 36-37 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0.3418332272938258 2311 50-51 0.22282699598300226 2311 52-53 0.6184391227679527 2311 54-55 0.7545202559526736 2311 56-57 0.902770291616303 2311 58-59 1.2120204314317888 2311 60-61 1.5790168144775585 2311 62-63 1.5214384255379265 2311 64-65 1.9905917056357616 2311 66-67 2.511669054217286 2311 68-69 2.9767619188390277 2311 70-71 3.5208357800941954 2311 72-73 3.5104601935448336 2311 74-75 3.8021311188633824 2311 76-77 3.5223375499231473 2311 78-79 3.6246823361189726 2311 80-81 3.40844866490718 2311 82-83 3.0797641555123683 2311 84-85 3.4077526807552125 2311 86-87 3.584139080910461 2311 88-89 3.4616018870589897 2311 90-91 3.6088093691689753 2311 92-93 3.378871104910969 2311 94-95 2.8590442917586127 2311 96-97 2.4261277645273225 2311 98-99 2.407773218557903 2311 100-101 2.352030338605177 2312 1 -0.1319966394579133 2312 2 -0.22358046765316075 2312 3 -0.15380892326708206 2312 4 -0.10544848301140775 2312 5 -0.12994838962498534 2312 6 -0.06404323029580894 2312 7 -0.1471257615179482 2312 8 -0.0867699861291058 2312 9 -0.1894300483207374 2312 10-11 -0.3002872699545378 2312 12-13 -0.10056058566673443 2312 14-15 -0.15808173997394448 2312 16-17 -0.2026122974051745 2312 18-19 -0.2130649341630999 2312 20-21 -0.15799725841227286 2312 22-23 -0.3132234845984456 2312 24-25 -0.11530996010178995 2312 26-27 -0.18355354333592544 2312 28-29 -0.07852568836234042 2312 30-31 0.06063740322637301 2312 32-33 -0.06642233610042325 2312 34-35 0.012077024185813912 2312 36-37 -0.05994106148570921 2312 38-39 0.25732548331338734 2312 40-41 0.5306617343019084 2312 42-43 0.8741059774568356 2312 44-45 0.8250199016523894 2312 46-47 0.9413547687044854 2312 48-49 1.13312151172304 2312 50-51 1.1680576544632935 2312 52-53 1.1680497341289353 2312 54-55 1.2816034405508532 2312 56-57 1.2992776926776735 2312 58-59 1.676757110711197 2312 60-61 2.0284150320354435 2312 62-63 2.2085701572910956 2312 64-65 2.437813332917358 2312 66-67 2.49948615757037 2312 68-69 2.8727335758676595 2312 70-71 2.949396202264065 2312 72-73 3.219928078649904 2312 74-75 3.2032430628913673 2312 76-77 3.611306458326087 2312 78-79 3.634003270889682 2312 80-81 3.583390063255628 2312 82-83 3.6004963489394655 2312 84-85 3.817474605507119 2312 86-87 3.8994201308616745 2312 88-89 3.5877394135338463 2312 90-91 3.1536809809821182 2312 92-93 2.9822905261527293 2312 94-95 2.5454180691556285 2312 96-97 2.0992064037791245 2312 98-99 2.112679693891664 2312 100-101 1.6643935022614453 2313 1 -0.1623708140727338 2313 2 -0.22247723676269615 2313 3 -0.2809630766868274 2313 4 -0.15061573390132565 2313 5 -0.06574904721581731 2313 6 -0.16452419548696895 2313 7 -0.07167672480699139 2313 8 -0.06469449904995628 2313 9 -0.27375384386977686 2313 10-11 -0.1826709008341325 2313 12-13 -0.18985163754032186 2313 14-15 -0.2350850822744306 2313 16-17 -0.29573204958582266 2313 18-19 -0.22555644254602214 2313 20-21 -0.25165232213336 2313 22-23 -0.11215014420008629 2313 24-25 -0.07680285431661815 2313 26-27 0.002224017056455807 2313 28-29 0.023278610433891345 2313 30-31 0.03238001891616449 2313 32-33 0.271617391080909 2313 34-35 0.3092129715785461 2313 36-37 0.2713678703367961 2313 38-39 0.3887268039296785 2313 40-41 0.41819468177616415 2313 42-43 0.677551536001431 2313 44-45 0.8802982908265946 2313 46-47 0.8226487552809374 2313 48-49 0.8969023872427329 2313 50-51 1.088260909809609 2313 52-53 1.271171931895303 2313 54-55 1.6712667649170392 2313 56-57 2.294027835336845 2313 58-59 2.522679775856165 2313 60-61 2.685894448355654 2313 62-63 2.8974671981323787 2313 64-65 2.9434327454333236 2313 66-67 3.2999263629995745 2313 68-69 3.782138211611251 2313 70-71 3.8160818901529794 2313 72-73 4.14728603087946 2313 74-75 4.248866823115275 2313 76-77 4.332170021816207 2313 78-79 4.079458557430808 2313 80-81 4.196949477401983 2313 82-83 4.344606563335812 2313 84-85 4.274342625397885 2313 86-87 4.4484187993761175 2313 88-89 4.625298175524009 2313 90-91 4.488446340619079 2313 92-93 4.25374782350878 2313 94-95 4.190609775902727 2313 96-97 4.055294553557665 2313 98-99 3.3977534690608167 2313 100-101 2.8035880893650553 2314 1 -0.10119639976733197 2314 2 -0.2799785719185657 2314 3 -0.311567089997304 2314 4 -0.24013279962087353 2314 5 -0.24065820059627896 2314 6 -0.3695089018833926 2314 7 -0.14385543775408394 2314 8 -0.23644222741762633 2314 9 -0.3278640698565738 2314 10-11 -0.20047702499802966 2314 12-13 -0.14091303977143355 2314 14-15 -0.21188138043807925 2314 16-17 -0.1139930604201922 2314 18-19 -0.2422626970510109 2314 20-21 -0.08374946919471427 2314 22-23 -0.0763483597595993 2314 24-25 0.21326769419902547 2314 26-27 -0.10596188892615999 2314 28-29 -0.0175090008135399 2314 30-31 0.04716570305750167 2314 32-33 0.32112646453875016 2314 34-35 0.4917605236683045 2314 36-37 0.2358135098857943 2314 38-39 0.19066063248445886 2314 40-41 0.48768721050535646 2314 42-43 0.6782424558211275 2314 44-45 0.5742581577218289 2314 46-47 0.7369186771136285 2314 48-49 1.1421126322765076 2314 50-51 1.3653057523732564 2314 52-53 1.596446712574945 2314 54-55 1.6863082761267485 2314 56-57 1.8087164172332528 2314 58-59 2.2244918531346123 2314 60-61 2.2792666599339775 2314 62-63 2.8526087620013776 2314 64-65 3.1194025541045534 2314 66-67 3.171541322720813 2314 68-69 3.6330974697501013 2314 70-71 4.093367620978089 2314 72-73 4.218488119324665 2314 74-75 4.251140322792224 2314 76-77 4.4315793787379185 2314 78-79 4.085206263390273 2314 80-81 4.1335407777877755 2314 82-83 4.212501760135922 2314 84-85 4.483019084452483 2314 86-87 4.491595686054062 2314 88-89 4.524588208725508 2314 90-91 4.512825539824336 2314 92-93 4.28723596022874 2314 94-95 4.474540474777461 2314 96-97 4.693965896070896 2314 98-99 4.598979198167962 2314 100-101 3.9632213790023787 2315 1 -0.18827522554517273 2315 2 -0.358034697703868 2315 3 -0.30255197901436404 2315 4 -0.3322469332658642 2315 5 -0.2879801023514901 2315 6 -0.13255535800563933 2315 7 -0.07022623423328866 2315 8 -0.15560789170260847 2315 9 -0.26363132505093745 2315 10-11 -0.2724363301989001 2315 12-13 -0.2991056285976299 2315 14-15 -0.21693249874188325 2315 16-17 -0.1913468866091037 2315 18-19 -0.29374495710865745 2315 20-21 -0.2176784132978682 2315 22-23 -0.1631239413623291 2315 24-25 -0.0753119847099768 2315 26-27 0.060466040146948785 2315 28-29 0.045898755407236536 2315 30-31 -0.023965451471177346 2315 32-33 0.237608781513984 2315 34-35 0.561175613402014 2315 36-37 0.45758713731854783 2315 38-39 0.5053439747348776 2315 40-41 0.5304313584303273 2315 42-43 0.47632230990976865 2315 44-45 0.6762854351519891 2315 46-47 0.9173416885085288 2315 48-49 0.9559425271560755 2315 50-51 1.2515316367142226 2315 52-53 1.4410298040085934 2315 54-55 1.8792601099476762 2315 56-57 2.2246162777219567 2315 58-59 2.64701438933103 2315 60-61 2.745191058220378 2315 62-63 3.1003453398892837 2315 64-65 3.706724860606947 2315 66-67 3.8121470142250438 2315 68-69 4.029746542562851 2315 70-71 4.220638790204145 2315 72-73 4.549697794805969 2315 74-75 4.800525486531427 2315 76-77 4.990708830798777 2315 78-79 5.144986156815282 2315 80-81 5.218431122923285 2315 82-83 5.618987770429147 2315 84-85 5.564358089305653 2315 86-87 5.387900723765114 2315 88-89 5.497335442847721 2315 90-91 5.543098415312722 2315 92-93 5.476571314768295 2315 94-95 5.032783593759218 2315 96-97 4.962329085832813 2315 98-99 4.9939732344712535 2315 100-101 4.557902178934583 2316 1 -0.35404202702054377 2316 2 -0.33127277534546806 2316 3 -0.23700963060572278 2316 4 -0.1607387865775749 2316 5 -0.12160768818083767 2316 6 -0.2780721132902073 2316 7 -0.23362738250233406 2316 8 -0.26920440994284434 2316 9 -0.2648795030805431 2316 10-11 -0.36079419597871265 2316 12-13 -0.5158715199431825 2316 14-15 -0.2279019580700279 2316 16-17 -0.2468504347467615 2316 18-19 -0.4097050756308249 2316 20-21 -0.39768190326932285 2316 22-23 -0.2512724088542626 2316 24-25 -0.3613606527041995 2316 26-27 -0.29161425656905493 2316 28-29 -0.40964858845075725 2316 30-31 -0.43111031265927835 2316 32-33 -0.2844594713789377 2316 34-35 0.031838297395367476 2316 36-37 -0.004606549254638992 2316 38-39 0.249146880307201 2316 40-41 -0.004660988951847855 2316 42-43 0.0928209789304546 2316 44-45 0.32068745249235775 2316 46-47 0.17894687008968901 2316 48-49 0.8130507778592033 2316 50-51 0.9253373479498741 2316 52-53 1.0881204679927734 2316 54-55 1.5002447609163134 2316 56-57 2.0022838818907616 2316 58-59 2.1951626515305307 2316 60-61 2.669573299055781 2316 62-63 2.712342630029088 2316 64-65 3.1196435716441506 2316 66-67 3.774567206789353 2316 68-69 3.9230577727435794 2316 70-71 4.156204336658405 2316 72-73 4.459833766831455 2316 74-75 4.699706369699502 2316 76-77 4.853216272649693 2316 78-79 5.285977924588476 2316 80-81 5.378924988100895 2316 82-83 5.144542915399825 2316 84-85 5.249629041109831 2316 86-87 5.3794820229925335 2316 88-89 5.3219127115002465 2316 90-91 5.18461231018931 2316 92-93 5.240557546488715 2316 94-95 4.797068525978501 2316 96-97 4.7372700642565775 2316 98-99 4.200581255931157 2316 100-101 3.6473142213890597 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 29.0 5 51.0 6 152.0 7 340.0 8 949.0 9 1726.0 10 2863.0 11 4648.0 12 7150.0 13 10519.0 14 14319.0 15 19796.0 16 25604.0 17 32174.0 18 35194.0 19 35982.0 20 38266.0 21 40910.0 22 42683.0 23 43886.0 24 44391.0 25 45546.0 26 47903.0 27 52140.0 28 57632.0 29 62226.0 30 67183.0 31 70876.0 32 75196.0 33 80383.0 34 85016.0 35 85795.0 36 71544.0 37 41348.0 38 8270.0 39 112.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.68741040156065 19.682282736755393 15.281941743853029 22.348365117830927 2 17.64023578323525 21.06546305668824 37.471940070696654 23.822361089379857 3 20.95855989323082 23.62874721822228 29.98580774523546 25.426885143311438 4 14.95987758532748 17.423257350986717 34.74314550471706 32.87371955896873 5 14.24821780421974 37.01486002112587 33.913176183696 14.82374599095838 6 33.75345522987905 35.75726905335282 15.906309890318651 14.582965826449481 7 29.407205550393833 32.699000328860194 19.435557769242312 18.458236351503658 8 27.284069067247334 34.96186167393567 18.524227176071673 19.22984208274532 9 26.440838689284586 16.49297739804536 19.350549004958445 37.71563490771161 10-11 25.864495231527158 26.335196342308343 26.10611169113961 21.69419673502489 12-13 26.725837555834396 23.455873666599615 26.952208312180918 22.866080465385068 14-15 23.106837344180406 24.999900224375423 25.238962620858214 26.65429981058595 16-17 22.5540623812671 28.125568720606125 26.462988860259962 22.857380037866815 18-19 22.729547609697224 26.13572507287606 29.040710018741837 22.09401729868488 20-21 22.84804104686098 25.344824283292304 29.714474318572936 22.092660351273775 22-23 22.826132506390646 25.22187167621522 29.696142464025797 22.255853353368334 24-25 22.8007642054287 25.584758036868482 29.562234171413017 22.052243586289798 26-27 22.229761282314296 25.847525613956662 29.40761613589466 22.515096967834378 28-29 22.450694916715356 26.25713195595189 29.274863267593222 22.01730985973953 30-31 22.490764775560898 25.812292666417626 29.202506042407002 22.494436515614474 32-33 22.242084207367107 25.691045424213687 29.31130129660316 22.755569071816044 34-35 22.981696929637593 25.952193925692473 28.953265223687342 22.11284392098259 36-37 23.111479530443617 25.43850791766759 29.06498605722624 22.385026494662554 38-39 22.575385659262807 25.83675587219861 28.987327625775812 22.60053084276278 40-41 22.962087814496318 25.731391656804615 28.993232604040482 22.31328792465859 42-43 22.746303708185724 25.809304709189924 28.753366614993354 22.691024967631005 44-45 22.749058985664174 25.991632146510472 28.98459853580524 22.274710332020113 46-47 23.33630265355057 26.318593691631303 28.14684086678608 22.198262788032043 48-49 23.123980293930774 26.48665561951833 27.906541444366752 22.482822642184143 50-51 23.10163057186553 26.59102083951942 27.937272312206463 22.37007627640859 52-53 23.548625013170373 26.326256453482245 27.831749695884138 22.29336883746324 54-55 23.136232730848683 26.59565042480436 27.809240332947006 22.458876511399957 56-57 23.167841623483813 26.708516521233832 27.632837169503482 22.490804685778873 58-59 23.09692116614464 26.36073888181148 27.67761643406992 22.864723517973964 60-61 22.836067981468887 26.55645859075424 27.95267765634429 22.654795771432585 62-63 23.051343697218737 26.599761177255644 27.974508545575873 22.374386579949746 64-65 22.74623008081021 26.636199206265587 27.76641666906128 22.851154043862927 66-67 22.76597298633752 26.6976929072673 27.698323090363626 22.838011016031555 68-69 23.294094639817526 26.285747354431365 27.718135939224258 22.702022066526848 70-71 23.13435695060392 26.48873095085296 27.252851984176395 23.12406011436672 72-73 23.08498801097052 26.722525007742583 27.127334348649278 23.065152632637616 74-75 23.16947794242073 26.95775583247925 26.897810685082842 22.974955540017177 76-77 23.11005162785797 26.59102083951942 27.495865301417933 22.80306223120468 78-79 23.149403102779985 26.822819385510353 27.38655121438811 22.641226297321545 80-81 23.34974044358664 26.669436439110306 27.13319372722371 22.847629390079348 82-83 23.401205950248567 26.889106536780368 27.05353978486384 22.65614772810723 84-85 23.204227825548315 26.965279529482384 27.025108002567155 22.805384642402142 86-87 23.55538242823309 26.780951985638236 26.684166539614985 22.979499046513688 88-89 23.16927839133086 27.156987640603695 26.33954655606747 23.33418741199797 90-91 23.263516800788008 26.877828451603968 26.737740399677996 23.12091434793003 92-93 23.384762991080464 26.5674045935881 26.64766407396484 23.400168341366598 94-95 23.192156202269853 26.38593276003613 26.767714057541774 23.654196980152243 96-97 23.438212148520407 26.034014707725266 26.668906962023804 23.858866181730523 98-99 23.15083987062704 26.376942430308777 26.214946855553745 24.25727084351044 100-101 23.90801652602306 25.637805193436847 26.385084114775402 24.069094165764696 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 530.0 1 388.5 2 349.0 3 470.5 4 388.5 5 344.0 6 691.5 7 1540.5 8 2436.0 9 2747.5 10 2187.0 11 1479.5 12 1298.5 13 1381.5 14 1493.0 15 1592.5 16 1673.5 17 1789.5 18 2031.0 19 2284.5 20 2560.5 21 2989.0 22 3644.5 23 4482.5 24 5422.5 25 6641.5 26 8057.0 27 9514.5 28 10870.0 29 12109.5 30 13831.0 31 16441.0 32 19443.0 33 20573.5 34 20501.5 35 21578.5 36 23703.0 37 25723.5 38 27414.5 39 29214.5 40 31178.5 41 33399.5 42 34900.5 43 35894.0 44 37064.5 45 39292.0 46 41417.5 47 42947.0 48 43401.5 49 42901.0 50 43221.0 51 43248.5 52 43811.0 53 45003.0 54 45864.0 55 44867.5 56 42302.0 57 39501.5 58 36625.5 59 32880.0 60 27704.5 61 23442.0 62 20345.0 63 16705.5 64 12768.0 65 9702.5 66 7410.0 67 5720.0 68 4452.5 69 3471.5 70 2729.0 71 2047.5 72 1474.0 73 1073.5 74 764.5 75 548.5 76 401.0 77 275.5 78 189.5 79 126.5 80 78.5 81 46.0 82 28.5 83 19.0 84 11.0 85 6.0 86 3.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010855579288831844 3 0.0012771269751566876 4 0.0016762291548931526 5 0.1010526719092729 6 7.982043594729297E-5 7 0.0 8 3.192817437891719E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 7.982043594729297E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.5919196176281834E-4 24-25 5.986532696046973E-4 26-27 0.00614617356794156 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010057374929358914 36-37 9.97755449341162E-4 38-39 0.006944377927414488 40-41 0.009139439915965046 42-43 0.005587430516310508 44-45 0.0033125480918126584 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.1973065392093945E-4 68-69 7.582941414992833E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.1973065392093945E-4 82-83 0.0019156904627350311 84-85 0.00510850790062675 86-87 0.0032726378738390117 88-89 0.0 90-91 0.00227488242449785 92-93 3.9910217973646486E-5 94-95 3.9910217973646486E-5 96-97 7.982043594729297E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1252812.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.050497849491634 #Duplication Level Percentage of deduplicated Percentage of total 1 82.13500434435717 50.9651791043752 2 10.647616273986053 13.213797814223675 3 3.0488916705150775 5.675557381338863 4 1.3169376166213556 3.268665389923122 5 0.725036173338238 2.249442775726399 6 0.4490024017652497 1.6716493539090722 7 0.3022994095880015 1.3130480205180013 8 0.23005239151345272 1.1419892339900723 9 0.162498699201699 0.9074812666824189 >10 0.8969275993161335 9.988603268502146 >50 0.05116642834138235 2.1670058079001637 >100 0.029407756054314158 3.5628493018519904 >500 0.003353503011297531 1.4344365008142193 >1k 0.0015477706205988602 1.5159904144631446 >5k 2.5796177009981006E-4 0.9243043657815889 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6116 0.48818178625364383 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5433 0.43366442850164266 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3518 0.28080829366257665 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1846 0.14734852475870283 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1643 0.13114497626140234 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1607 0.1282714405672998 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1522 0.1214867035117799 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1501 0.11981047435688674 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1487 0.11869298825362465 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1435 0.11454232558436542 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.982043594729297E-5 0.0 0.0 0.0 0.0 3 7.982043594729297E-5 0.0 0.0 0.0 0.0 4 7.982043594729297E-5 0.0 0.0 0.0 0.0 5 1.5964087189458594E-4 0.0 0.0 0.0 0.0 6 1.5964087189458594E-4 0.0 0.0 0.0 0.0 7 1.5964087189458594E-4 0.0 0.0 0.0 0.0 8 1.5964087189458594E-4 0.0 0.0 0.0 0.0 9 1.5964087189458594E-4 0.0 0.0 0.0 0.0 10-11 1.5964087189458594E-4 0.0 0.0 0.0 0.0 12-13 2.3946130784187892E-4 0.0 0.0 0.0 0.0 14-15 2.3946130784187892E-4 0.0 0.0 0.0 0.0 16-17 2.793715258155254E-4 0.0 0.0 0.0 7.982043594729297E-5 18-19 3.991021797364649E-4 0.0 0.0 0.0 7.982043594729297E-5 20-21 4.7892261568375783E-4 0.0 0.0 0.0 7.982043594729297E-5 22-23 5.587430516310508E-4 0.0 0.0 7.982043594729297E-5 1.1973065392093946E-4 24-25 6.385634875783438E-4 0.0 0.0 7.982043594729297E-5 1.5964087189458594E-4 26-27 6.784737055519903E-4 0.0 0.0 1.9955108986823244E-4 1.5964087189458594E-4 28-29 9.97755449341162E-4 0.0 0.0 0.0010376656673148086 1.5964087189458594E-4 30-31 0.0011973065392093946 0.0 0.0 0.003951111579391002 1.5964087189458594E-4 32-33 0.001237216757183041 0.0 0.0 0.01261162887967229 1.5964087189458594E-4 34-35 0.001317037193130334 0.0 0.0 0.02654029495247491 1.5964087189458594E-4 36-37 0.0014367678470512736 0.0 0.0 0.05112498922424115 1.5964087189458594E-4 38-39 0.0015165882829985663 0.0 0.0 0.0862858912590237 1.5964087189458594E-4 40-41 0.0015165882829985663 0.0 0.0 0.12475934138561892 1.5964087189458594E-4 42-43 0.0015964087189458593 0.0 0.0 0.16395117543573975 1.5964087189458594E-4 44-45 0.0018358700267877383 0.0 0.0 0.21591427923742748 1.5964087189458594E-4 46-47 0.002115241552603264 0.0 0.0 0.2759392470697918 2.3946130784187892E-4 48-49 0.0021950619885505564 0.0 0.0 0.336203676209998 2.3946130784187892E-4 50-51 0.002234972206524203 0.0 0.0 0.40548781461224825 2.3946130784187892E-4 52-53 0.002234972206524203 0.0 0.0 0.47165895601255414 2.3946130784187892E-4 54-55 0.002234972206524203 0.0 0.0 0.5426592337876712 2.3946130784187892E-4 56-57 0.002394613078418789 0.0 0.0 0.6260715893525924 2.3946130784187892E-4 58-59 0.002394613078418789 0.0 0.0 0.7147920039080086 2.3946130784187892E-4 60-61 0.002394613078418789 0.0 0.0 0.8028738549758463 2.3946130784187892E-4 62-63 0.002394613078418789 0.0 0.0 0.8888803747090546 2.3946130784187892E-4 64-65 0.002434523296392436 0.0 0.0 0.984106154794175 2.3946130784187892E-4 66-67 0.002713894822207961 0.0 0.0 1.089788411988391 2.3946130784187892E-4 68-69 0.002713894822207961 0.0 0.0 1.2064858893433332 2.3946130784187892E-4 70-71 0.002793715258155254 0.0 0.0 1.3257775308665627 2.3946130784187892E-4 72-73 0.002873535694102547 0.0 0.0 1.4495391168028404 2.3946130784187892E-4 74-75 0.0029533561300498397 0.0 0.0 1.5748172910221165 2.3946130784187892E-4 76-77 0.0029533561300498397 3.9910217973646486E-5 0.0 1.7133057473906699 2.3946130784187892E-4 78-79 0.0029932663480234864 7.982043594729297E-5 0.0 1.8707515572967053 2.3946130784187892E-4 80-81 0.0033524583097863047 7.982043594729297E-5 0.0 2.040250253030782 2.3946130784187892E-4 82-83 0.0034322787457335977 7.982043594729297E-5 0.0 2.215216648627248 2.3946130784187892E-4 84-85 0.0035919196176281837 7.982043594729297E-5 0.0 2.37645392924078 2.793715258155254E-4 86-87 0.0036717400535754767 7.982043594729297E-5 0.0 2.5385293244317584 3.192817437891719E-4 88-89 0.0036717400535754767 7.982043594729297E-5 0.0 2.7092253267050443 3.192817437891719E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2660 0.0 63.098705 1 TGGTATC 870 0.0 43.721806 2 GTATCAA 4635 0.0 42.05925 1 GTGGTAT 985 0.0 38.617233 1 TATCAAC 6205 0.0 31.340715 2 ATCAACG 6205 0.0 31.33696 3 TCAACGC 6315 0.0 30.791107 4 CAACGCA 6450 0.0 30.14664 5 AACGCAG 6665 0.0 29.145058 6 TAAGGTG 845 0.0 27.005976 5 ACGCAGA 7745 0.0 24.958286 7 ATACCGT 115 1.2828821E-4 24.779652 6 CGCAGAG 7760 0.0 24.726429 8 GTAAGGT 825 0.0 24.203081 4 AGGTAAG 770 0.0 23.464985 2 GAGTACT 6030 0.0 22.565716 12-13 GCAGAGT 8475 0.0 22.304123 9 GTGTAGG 435 0.0 21.860903 1 CAGAGTA 8050 0.0 21.623196 10-11 GTACTTT 6310 0.0 21.45148 14-15 >>END_MODULE