FastQCFastQC Report
Thu 26 May 2016
SRR938210_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938210_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237596
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60060.48529568615283175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49110.39681770141467815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31890.25767698021002006No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA21020.1698454099722365No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20230.16346206678108205No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA18060.1459280734585438No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA17690.14293840639433222No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA14660.11845545719281576No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA14510.11724343000462185No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC14020.11328414118985516No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG13190.10657759074851567No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13010.105123158122683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23450.056.992881
GTATCAA39650.043.9395031
TGGTATC7150.034.562222
ATCAACG50650.033.965143
TCAACGC51250.033.6602334
TATCAAC51400.033.6544572
CAACGCA53050.032.607715
AACGCAG54350.031.9139166
GTGGTAT9700.029.0325851
ACGCAGA62600.027.243727
CGCAGAG62750.027.1028888
TAAGGTG8000.026.1376785
AGGTAAG7750.025.1412282
GTAAGGT8600.024.8667144
GCAGAGT70250.023.7350149
GAGTACT47850.023.28176912-13
CTAACGA1050.002190130522.6300264
TTACGTA1050.002190130522.6300264
TACATGG38100.022.5765692
AGGCGAC5750.022.3151725
Produced by FastQC (version 0.11.5)