##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938210_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1237596 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37673521892443 33.0 31.0 34.0 31.0 34.0 2 32.58884320893086 34.0 31.0 34.0 31.0 34.0 3 32.67928548573202 34.0 31.0 34.0 31.0 34.0 4 36.18168610758276 37.0 37.0 37.0 35.0 37.0 5 36.18643887019674 37.0 37.0 37.0 35.0 37.0 6 36.23547264212231 37.0 37.0 37.0 35.0 37.0 7 36.200885426261884 37.0 37.0 37.0 35.0 37.0 8 36.17710464481139 37.0 37.0 37.0 35.0 37.0 9 37.9070270104299 39.0 38.0 39.0 35.0 39.0 10-11 37.83180617907621 39.0 38.0 39.0 35.0 39.0 12-13 37.754305524581525 39.0 38.0 39.0 35.0 39.0 14-15 39.05932953888022 40.0 38.5 41.0 36.0 41.0 16-17 38.90807339390237 40.0 38.0 41.0 36.0 41.0 18-19 39.04541546675975 40.0 39.0 41.0 36.0 41.0 20-21 39.03140160440079 40.0 39.0 41.0 36.0 41.0 22-23 38.951310443795876 40.0 39.0 41.0 36.0 41.0 24-25 38.85302311901461 40.0 38.5 41.0 35.5 41.0 26-27 38.68791350327571 40.0 38.0 41.0 35.0 41.0 28-29 38.496317053384146 40.0 38.0 41.0 35.0 41.0 30-31 38.28318328436744 40.0 38.0 41.0 34.0 41.0 32-33 38.03191509991952 40.0 38.0 41.0 34.0 41.0 34-35 37.81382575573935 40.0 37.5 41.0 33.5 41.0 36-37 37.56381403947653 40.0 37.0 41.0 33.0 41.0 38-39 37.27047841137173 39.5 36.5 41.0 32.5 41.0 40-41 37.059158239037615 39.0 36.0 41.0 32.0 41.0 42-43 36.88909547218963 39.0 36.0 41.0 31.0 41.0 44-45 36.633331070882576 39.0 36.0 41.0 31.0 41.0 46-47 36.25284745587413 39.0 35.5 40.0 30.0 41.0 48-49 35.845757420030445 39.0 35.0 40.0 29.0 41.0 50-51 35.147681472790794 38.5 34.5 39.5 28.0 40.5 52-53 35.297211287043595 38.0 35.0 40.0 28.0 40.5 54-55 35.40356222870791 38.0 35.0 40.0 28.0 41.0 56-57 35.09490172883558 38.0 35.0 40.0 27.5 41.0 58-59 34.76029091884589 38.0 34.0 40.0 26.5 41.0 60-61 34.323699333223445 37.5 34.0 40.0 26.0 41.0 62-63 33.77258249057043 36.5 33.5 39.5 24.5 41.0 64-65 33.23632388921749 36.0 33.0 39.0 22.5 40.5 66-67 32.690188478307945 35.5 32.0 39.0 21.5 40.0 68-69 32.07373569403909 35.0 31.5 38.0 19.0 40.0 70-71 31.501725118697863 35.0 31.0 37.5 16.0 39.5 72-73 30.843738182734917 34.5 30.5 37.0 9.0 39.0 74-75 30.154350854398366 34.0 29.5 36.0 6.0 39.0 76-77 29.476449503715266 34.0 29.0 35.5 2.0 38.0 78-79 28.75767294011939 34.0 28.0 35.0 2.0 37.0 80-81 28.099792662549007 33.0 26.5 35.0 2.0 36.5 82-83 27.355294053956218 33.0 25.5 35.0 2.0 36.0 84-85 26.696453850852784 32.0 25.0 35.0 2.0 35.5 86-87 25.993539087068804 32.0 23.5 34.5 2.0 35.0 88-89 25.132847876043556 31.0 19.5 34.0 2.0 35.0 90-91 24.255253329842695 31.0 15.5 34.0 2.0 35.0 92-93 23.322897779242986 30.0 4.5 34.0 2.0 35.0 94-95 22.30533186920449 29.5 2.0 34.0 2.0 35.0 96-97 21.0177230695639 29.0 2.0 34.0 2.0 35.0 98-99 19.41350165966923 27.5 2.0 33.5 2.0 35.0 100-101 15.611144105184568 12.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3208737663905836 1101 2 0.3104267724057692 1101 3 0.21657463962868917 1101 4 0.1725101912107121 1101 5 0.20221559026077784 1101 6 0.1913522175414002 1101 7 0.26073118362049286 1101 8 0.22357078989694656 1101 9 0.2844563474322328 1101 10-11 0.3624934122485328 1101 12-13 0.41559608247904123 1101 14-15 0.46788561132762396 1101 16-17 0.5992656559636487 1101 18-19 0.45559047071923686 1101 20-21 0.4787298519806811 1101 22-23 0.4853919631098549 1101 24-25 0.5592926233917197 1101 26-27 0.5774618644618741 1101 28-29 0.5039319339167818 1101 30-31 0.5529862856572976 1101 32-33 0.6104860473316833 1101 34-35 0.5993410688746863 1101 36-37 0.6205709956592855 1101 38-39 0.6846222172716878 1101 40-41 0.8220568464659692 1101 42-43 0.9796085314497489 1101 44-45 0.908001810949564 1101 46-47 1.1092965303658318 1101 48-49 1.2238009228588425 1101 50-51 1.1568518883988972 1101 52-53 1.1947473316942592 1101 54-55 1.177590397038827 1101 56-57 1.132818944260535 1101 58-59 1.294448699031328 1101 60-61 1.1731863688079471 1101 62-63 1.2650592216801968 1101 64-65 1.1523462646854483 1101 66-67 1.2108321094534418 1101 68-69 1.3355712907176098 1101 70-71 1.531375908482179 1101 72-73 1.6500617374087234 1101 74-75 1.9778514510143914 1101 76-77 2.102847882216121 1101 78-79 2.232964657309818 1101 80-81 2.5636944626690834 1101 82-83 2.8639384584142036 1101 84-85 2.6741888904601048 1101 86-87 3.024491763625715 1101 88-89 3.470046273857985 1101 90-91 3.8664412678102167 1101 92-93 3.8994294330265937 1101 94-95 4.369122594182102 1101 96-97 4.775946400516187 1101 98-99 4.842711642228199 1101 100-101 4.476571482736199 1102 1 0.2603009314079543 1102 2 0.22048585092885276 1102 3 0.12205478563637939 1102 4 0.04824635957293566 1102 5 0.11225385019702827 1102 6 0.14103022339836002 1102 7 -0.0239294502823455 1102 8 0.12907869402041428 1102 9 0.20334135018491395 1102 10-11 0.11852501948970229 1102 12-13 0.1890858096630268 1102 14-15 0.3522771071662234 1102 16-17 0.41663922931216035 1102 18-19 0.3224278497979398 1102 20-21 0.32617124765874195 1102 22-23 0.35289958502580987 1102 24-25 0.2836892313499675 1102 26-27 0.32559917923428117 1102 28-29 0.4065333320005493 1102 30-31 0.3316783463735504 1102 32-33 0.48437861417091455 1102 34-35 0.44015694929272087 1102 36-37 0.4971936888441988 1102 38-39 0.48516916781134967 1102 40-41 0.6027081675209729 1102 42-43 0.7933683451002693 1102 44-45 0.6405783886514698 1102 46-47 0.607611381420412 1102 48-49 0.9012180764491191 1102 50-51 0.9111214335821742 1102 52-53 0.7898686115284477 1102 54-55 0.7485291533827123 1102 56-57 0.8143101796354699 1102 58-59 0.7641038738380601 1102 60-61 0.8022026211847333 1102 62-63 0.8214480990032058 1102 64-65 0.9338210765085577 1102 66-67 0.941341864117966 1102 68-69 1.1585334980429707 1102 70-71 1.2943961996648135 1102 72-73 1.445342163773386 1102 74-75 1.6355672819304523 1102 76-77 1.9781422339022008 1102 78-79 2.29188125805096 1102 80-81 2.299840271424916 1102 82-83 2.367314539245836 1102 84-85 2.5407972650836363 1102 86-87 2.7619734317034172 1102 88-89 3.170727041647467 1102 90-91 3.3563384702547836 1102 92-93 3.508540803213453 1102 94-95 3.7852340985331843 1102 96-97 3.954833577654604 1102 98-99 4.090453403850756 1102 100-101 4.3755047614785685 1103 1 0.30206923352618986 1103 2 0.3119633842188563 1103 3 0.26805956285428323 1103 4 0.1650490559720197 1103 5 0.18877936667392703 1103 6 0.2639929425300167 1103 7 0.22084529179571888 1103 8 0.20044155157322763 1103 9 0.2812609252432239 1103 10-11 0.305919691400625 1103 12-13 0.3160246201976662 1103 14-15 0.5113974294736963 1103 16-17 0.3218889641456144 1103 18-19 0.4272586636974509 1103 20-21 0.4869972168178336 1103 22-23 0.5388917580410038 1103 24-25 0.5974494757814739 1103 26-27 0.5826693491940773 1103 28-29 0.6491572006467479 1103 30-31 0.6898255004402358 1103 32-33 0.6437535526269542 1103 34-35 0.692549778401677 1103 36-37 0.840736989063366 1103 38-39 0.8186213689046582 1103 40-41 0.8260058264591237 1103 42-43 1.0690782458703083 1103 44-45 0.9272789853816974 1103 46-47 1.0348312544218174 1103 48-49 1.0484389622882446 1103 50-51 0.9802265070551073 1103 52-53 1.0595858891331957 1103 54-55 1.1101065918598536 1103 56-57 0.9891956023686177 1103 58-59 1.0946772243896348 1103 60-61 1.2685658585516961 1103 62-63 1.348343160575297 1103 64-65 1.3495501649264767 1103 66-67 1.539490762516273 1103 68-69 1.7339666971753118 1103 70-71 1.8952516255208671 1103 72-73 1.9382522997468534 1103 74-75 2.0045764171639853 1103 76-77 2.0224440370346812 1103 78-79 2.3745731274961805 1103 80-81 2.509705873486606 1103 82-83 2.7239494703306697 1103 84-85 2.671081956885555 1103 86-87 2.6381301064446525 1103 88-89 3.0428372890330806 1103 90-91 3.060818448422502 1103 92-93 3.528416336711654 1103 94-95 3.999575880621151 1103 96-97 3.956405586196201 1103 98-99 3.9955054946572837 1103 100-101 4.093673522749905 1104 1 0.21136554411640418 1104 2 0.2023447834727179 1104 3 0.19816999462829443 1104 4 0.10542266880403872 1104 5 0.15652936159582964 1104 6 0.17927599589070553 1104 7 0.19229146730843638 1104 8 0.15506093096155382 1104 9 0.26528792719998506 1104 10-11 0.2427677478420236 1104 12-13 0.1822958720511707 1104 14-15 0.3138816365789765 1104 16-17 0.4086374898035672 1104 18-19 0.3536888262643956 1104 20-21 0.4329929647544404 1104 22-23 0.34973418117547794 1104 24-25 0.30508016917926994 1104 26-27 0.3895244472691388 1104 28-29 0.3680734661434215 1104 30-31 0.3290423218197205 1104 32-33 0.31178758267576967 1104 34-35 0.31847246938906437 1104 36-37 0.3012564449617514 1104 38-39 0.4468874343241396 1104 40-41 0.5553843345806939 1104 42-43 0.6058944795442116 1104 44-45 0.6357763413836679 1104 46-47 0.40032217139147264 1104 48-49 0.596628449941683 1104 50-51 0.724654475882339 1104 52-53 0.5722276527915966 1104 54-55 0.7777871860654031 1104 56-57 1.0227476325934788 1104 58-59 0.9038926239007807 1104 60-61 0.8628052605544951 1104 62-63 1.0758085787061518 1104 64-65 1.2157025892119862 1104 66-67 1.1164727454132262 1104 68-69 1.2155182940688718 1104 70-71 1.477410901857592 1104 72-73 1.6914809564875206 1104 74-75 1.7061695867793354 1104 76-77 1.7223709123774533 1104 78-79 1.9494832451050037 1104 80-81 2.047399941587326 1104 82-83 2.1487837549616273 1104 84-85 2.3469189972327626 1104 86-87 2.567167704705909 1104 88-89 2.849338612724793 1104 90-91 3.2246752324271597 1104 92-93 3.5575815645829785 1104 94-95 3.749981451636703 1104 96-97 4.026375049881008 1104 98-99 4.189927369763076 1104 100-101 4.027224198814446 1105 1 0.18259991319560243 1105 2 0.12287655055620661 1105 3 0.07545779303678302 1105 4 0.0020659680335484154 1105 5 0.0807857086970003 1105 6 0.05750007066650653 1105 7 0.13262397962370187 1105 8 0.14252145893932777 1105 9 0.16608999869171015 1105 10-11 0.07752917893966327 1105 12-13 0.04319015018683103 1105 14-15 0.16944008674948918 1105 16-17 0.3186641728905997 1105 18-19 0.1891220711803996 1105 20-21 0.16052312137941982 1105 22-23 0.2031429850153117 1105 24-25 0.22267521780879207 1105 26-27 0.2886742199102912 1105 28-29 0.3744829331132351 1105 30-31 0.3495725100223197 1105 32-33 0.30610124315322906 1105 34-35 0.20485935836216385 1105 36-37 0.27285237776458615 1105 38-39 0.5988389311032236 1105 40-41 0.6539664995421717 1105 42-43 0.4891954458193126 1105 44-45 0.5814833642458694 1105 46-47 0.6219709967040998 1105 48-49 0.4987338876074716 1105 50-51 0.6495336914943124 1105 52-53 0.5136440668373154 1105 54-55 0.5884280538183404 1105 56-57 0.518077302923146 1105 58-59 0.5636137935357439 1105 60-61 0.6160459135796401 1105 62-63 0.4646932572459974 1105 64-65 0.7765558164445281 1105 66-67 0.8309376626798652 1105 68-69 0.9338838365723419 1105 70-71 1.1986681647096837 1105 72-73 1.511453799736227 1105 74-75 1.6121424931832742 1105 76-77 1.7334530773389254 1105 78-79 1.6159936025636377 1105 80-81 1.926314776752161 1105 82-83 2.10382748996226 1105 84-85 2.1781476823407573 1105 86-87 2.6186479055395573 1105 88-89 2.688514594788707 1105 90-91 2.7554160433403823 1105 92-93 2.9115350823296033 1105 94-95 3.425315384944774 1105 96-97 3.4069129745999653 1105 98-99 3.545483451612263 1105 100-101 3.7046343604918466 1106 1 8.787800213880814E-4 1106 2 0.07613353767384012 1106 3 -0.09772517549367876 1106 4 0.022242275314368953 1106 5 0.06554714793320215 1106 6 -0.06742163637393617 1106 7 -0.07879346150845379 1106 8 -0.05447420743956144 1106 9 0.051236343045303556 1106 10-11 -0.03289404740740309 1106 12-13 0.07415607370518984 1106 14-15 0.14579508395633667 1106 16-17 0.09255992475693375 1106 18-19 0.190118719395322 1106 20-21 0.1294677957653505 1106 22-23 -0.003566298413666402 1106 24-25 0.013637247839433542 1106 26-27 -0.011345988137378527 1106 28-29 -0.04384640093108061 1106 30-31 0.007898860470071156 1106 32-33 0.08125814647623031 1106 34-35 0.28198215557916484 1106 36-37 0.40516113134066956 1106 38-39 0.1505334446711828 1106 40-41 0.26072212393910377 1106 42-43 0.3710751751372783 1106 44-45 0.3934818660995063 1106 46-47 0.5691639825013297 1106 48-49 0.6820859477703181 1106 50-51 0.6065471536512987 1106 52-53 0.7508764953579643 1106 54-55 0.4331249754238158 1106 56-57 0.8282761247199062 1106 58-59 1.0001446536579621 1106 60-61 0.989405464474288 1106 62-63 0.9679350089769159 1106 64-65 1.0639692709838258 1106 66-67 1.2843327204896937 1106 68-69 1.1630240529148352 1106 70-71 1.155984218575238 1106 72-73 1.3908805259901875 1106 74-75 1.4355019382766336 1106 76-77 1.7664175873519632 1106 78-79 2.068940591881937 1106 80-81 2.084896637331447 1106 82-83 2.1432760324435947 1106 84-85 2.297022845274757 1106 86-87 2.285118310800847 1106 88-89 2.552826018789517 1106 90-91 2.8353489229977598 1106 92-93 3.1322577475065856 1106 94-95 3.1642766066535053 1106 96-97 3.431901653861221 1106 98-99 3.4007483357386796 1106 100-101 3.2209272311772725 1107 1 0.1696888767438054 1107 2 0.16178348894996475 1107 3 0.005626207223698998 1107 4 0.02441146016970208 1107 5 0.10044069651358711 1107 6 0.16546482490608838 1107 7 0.0732744386554316 1107 8 0.12179426900110002 1107 9 0.15766621272602066 1107 10-11 0.09690804009795073 1107 12-13 0.06368044037364484 1107 14-15 0.09397905522028793 1107 16-17 0.30181583180418414 1107 18-19 0.05799344384524119 1107 20-21 0.19884780941013247 1107 22-23 0.2598650923770549 1107 24-25 0.19317658382814074 1107 26-27 0.15028246156034442 1107 28-29 0.3210089674729275 1107 30-31 0.2822452930098436 1107 32-33 0.3431552870627499 1107 34-35 0.3385538869008897 1107 36-37 0.2949148208471115 1107 38-39 0.47010083762319255 1107 40-41 0.5205057941347064 1107 42-43 0.7385901208051635 1107 44-45 0.5843123164721149 1107 46-47 0.6268605152745792 1107 48-49 0.7749351539443197 1107 50-51 0.5940876123390311 1107 52-53 0.7496739038995202 1107 54-55 0.7760380429886382 1107 56-57 0.8334770174127968 1107 58-59 0.7809631622132898 1107 60-61 0.7655042746025487 1107 62-63 0.8057181409497574 1107 64-65 1.0760001788457672 1107 66-67 1.1656377845140256 1107 68-69 1.2434410494006158 1107 70-71 1.4481366785790932 1107 72-73 1.617987925606915 1107 74-75 1.7040180623579992 1107 76-77 1.8945587530877752 1107 78-79 1.9060927312648097 1107 80-81 2.0393813363524487 1107 82-83 2.0598433591689833 1107 84-85 2.185791545098887 1107 86-87 2.4455026459958837 1107 88-89 2.87706449141427 1107 90-91 2.9574053859281904 1107 92-93 3.1458074001321563 1107 94-95 3.72529892502698 1107 96-97 3.990290149770413 1107 98-99 4.08922222194872 1107 100-101 3.8649162711960248 1108 1 0.136443173762558 1108 2 0.07528875996999318 1108 3 -0.03486025769129242 1108 4 0.04517462581488019 1108 5 0.06886271352308881 1108 6 0.05964638692099555 1108 7 0.00249209578317533 1108 8 0.08116494539044083 1108 9 0.04365203348287139 1108 10-11 0.11854543884813751 1108 12-13 0.11971986937364676 1108 14-15 0.25044659446372464 1108 16-17 0.2657561748410373 1108 18-19 0.13886388808920458 1108 20-21 0.030073718180368303 1108 22-23 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-1.9606538232176263 2316 94-95 -2.313876599945136 2316 96-97 -2.2996041712388795 2316 98-99 -2.6601986338156145 2316 100-101 -2.824638686808031 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2395.0 3 471.0 4 496.0 5 496.0 6 628.0 7 730.0 8 954.0 9 1140.0 10 1519.0 11 2191.0 12 2961.0 13 4199.0 14 5250.0 15 6951.0 16 9036.0 17 11821.0 18 11206.0 19 9135.0 20 8981.0 21 9580.0 22 10682.0 23 12185.0 24 14021.0 25 16109.0 26 19074.0 27 22461.0 28 26619.0 29 32458.0 30 39729.0 31 49603.0 32 63205.0 33 83435.0 34 113610.0 35 156102.0 36 200056.0 37 202128.0 38 82784.0 39 3195.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.90674068611584 17.749416094930513 14.230084963359399 24.113758255594252 2 15.887601921586159 19.027033318971824 39.9629618552376 25.12240290420442 3 19.463702506063054 22.548666127728374 29.313257881972515 28.674373484236053 4 13.065485477943255 15.8321381854988 35.36435705358197 35.738019282975976 5 14.10783986311594 36.55389850299232 33.91203937680879 15.42622225708295 6 33.86192815412295 34.76992742636505 16.50765156569026 14.86049285382174 7 29.681773133005997 32.22315814661103 19.541292858286138 18.553775862096842 8 27.25403276653119 34.15281994574478 18.965966004804724 19.627181282919306 9 26.334258649986175 16.515042997314588 19.49704701654091 37.65365133615832 10-11 25.542639779181048 25.85253487886121 26.37721836509418 22.227606976863566 12-13 26.422745497461765 23.14134898308921 27.039762990267402 23.39614252918162 14-15 22.95744455554307 24.69839686567152 25.303583510017873 27.040575068767538 16-17 22.586396017210898 27.83013491786993 26.35146222323892 23.232006841680253 18-19 22.660440586894108 25.97375459205718 28.73111087668328 22.634693944365424 20-21 22.738971086074013 25.292237570277887 29.4198418790515 22.5489494645966 22-23 22.602566424046845 25.094418019614373 29.3808182679467 22.92219728839208 24-25 22.474464602544426 25.421560942757132 29.38186692689521 22.722107527803235 26-27 22.007727001902243 25.744708200177698 29.107344532285172 23.140220265634888 28-29 22.210438290843143 26.32149896996135 28.903284685546193 22.564778053649313 30-31 22.213994876591162 25.748645788789126 28.929152584298183 23.10820675032153 32-33 21.8429257496134 25.68211525450035 29.08426436316105 23.390694632725204 34-35 22.76315603331373 25.901687715107386 28.73986229085449 22.595293960724398 36-37 22.881615222291515 25.433389338850628 28.734791309053858 22.950204129804003 38-39 22.23079838904564 25.859688881812424 28.765945271842487 23.143567457299444 40-41 22.769165614981972 25.617382357802327 28.82416195580621 22.789290071409493 42-43 22.47421622246343 25.649898287331617 28.566671704481255 23.309213785723696 44-45 22.457568905575965 26.04823107470556 28.822515916206164 22.671684103512312 46-47 23.00210584338199 26.31879906714088 27.992724313135795 22.686370776341327 48-49 22.721413099702573 26.327872429846117 27.952242014742012 22.998472455709297 50-51 22.739016521685723 26.596487485089753 27.94960255502649 22.714893438198033 52-53 23.08864120976387 26.219685897469567 27.94802967677753 22.743643215989028 54-55 22.54524427951 26.526918669397137 28.024905567668828 22.902931483424034 56-57 22.631463254778776 26.705331101770728 27.839726717089636 22.823478926360856 58-59 22.614395511903325 26.22727454559193 27.854710638743207 23.303619303761536 60-61 22.19267953847683 26.47288911014473 28.20671240305128 23.127718948327153 62-63 22.46976625595351 26.46115788703819 28.342238426443178 22.726837430565126 64-65 22.19117536530904 26.55645773276859 28.09353980233995 23.158827099582425 66-67 22.111194973025913 26.642809119148207 28.05886783182651 23.187128075999368 68-69 22.663659402253668 26.145260245278994 28.25217706117752 22.938903291289815 70-71 22.454789299588725 26.434430038476677 27.787417375358924 23.323363286575674 72-73 22.396493948821057 26.60049854392686 27.834291724566807 23.168715782685275 74-75 22.405819811003223 26.918242144899686 27.62729321330485 23.048644830792245 76-77 22.65830876036691 26.57827941018416 27.659597679460774 23.10381414998816 78-79 22.609992181296935 26.78081569074302 27.71413043697863 22.895061690981418 80-81 22.91446191543534 26.734131549247625 27.401587784556863 22.949818750760173 82-83 22.744790946919096 26.9319944109202 27.32369871659664 22.999515925564058 84-85 22.615331714670955 27.00960004978013 27.380567081352936 22.994501154195977 86-87 22.912233338733795 26.934782758009078 27.121038084568 23.031945818689124 88-89 22.79057418338807 27.042868463942344 26.85968164369456 23.30687570897502 90-91 22.761425348784016 27.062495403026283 26.986438220310422 23.189641027879283 92-93 22.928216533536773 26.679869208364714 27.13146563567773 23.260448622420775 94-95 22.73163370546034 26.36262014917411 27.46829161049198 23.43745453487357 96-97 23.117691653654294 25.94832714235374 27.28068091076907 23.653300293222898 98-99 22.834015655629493 26.242596388884508 26.899783685445584 24.023604270040412 100-101 23.702464560813 25.15506667098194 27.100095475435303 24.04237329276976 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 591.0 1 437.0 2 388.0 3 505.0 4 416.5 5 367.5 6 699.0 7 1539.0 8 2483.0 9 2738.0 10 2110.5 11 1433.0 12 1245.5 13 1275.5 14 1359.0 15 1398.0 16 1491.5 17 1631.5 18 1732.5 19 1980.5 20 2278.0 21 2665.0 22 3260.5 23 3948.5 24 4831.5 25 5997.5 26 7370.5 27 8927.5 28 10260.5 29 11381.0 30 12734.5 31 15308.0 32 19208.5 33 20409.0 34 20022.5 35 21155.0 36 23103.5 37 25407.0 38 27358.0 39 28918.0 40 30823.5 41 33139.5 42 34737.5 43 36013.0 44 37206.5 45 39800.0 46 42176.0 47 43521.5 48 44167.5 49 44034.5 50 44303.0 51 44244.5 52 44916.5 53 46460.5 54 47940.0 55 46249.0 56 42618.0 57 39593.5 58 37304.5 59 33105.0 60 26618.0 61 22298.0 62 19028.5 63 14699.5 64 10443.0 65 7724.5 66 5819.5 67 4489.5 68 3577.0 69 2807.5 70 2208.0 71 1591.5 72 1118.0 73 826.0 74 618.0 75 453.5 76 301.5 77 169.5 78 115.5 79 82.5 80 61.5 81 40.0 82 22.0 83 15.0 84 12.0 85 8.5 86 5.0 87 3.0 88 3.0 89 4.0 90 3.0 91 1.0 92 1.0 93 1.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39972656666634343 2 0.0398352935853057 3 0.048157880277570383 4 0.05252117815506837 5 0.07506488385547465 6 0.07441846935510457 7 0.044764204150627505 8 0.038784870022204335 9 0.0437945824000724 10-11 0.04561262318236323 12-13 0.041693735273869666 14-15 0.03692642833364038 16-17 0.03821925733438053 18-19 0.04375418149379927 20-21 0.05102634462296258 22-23 0.04395618602516491 24-25 0.05934893131522727 26-27 0.05130915096687448 28-29 0.0409261180546802 30-31 0.04274415883697103 32-33 0.04242095158678599 34-35 0.04593583043254826 36-37 0.04165333436759654 38-39 0.025614174577164115 40-41 0.023876935607419547 42-43 0.023190120200776345 44-45 0.01438272263323411 46-47 0.02622018817126106 48-49 0.025533372764617857 50-51 0.015513948008881735 52-53 0.01595835797788616 54-55 0.016321966134344328 56-57 0.015917957071613028 58-59 0.015311943477516087 60-61 0.017372389697445693 62-63 0.015796754352793645 64-65 0.017695596947630732 66-67 0.009049803005181012 68-69 0.011958668256846336 70-71 0.018018804197815763 72-73 0.015554348915154864 74-75 0.013534303601498389 76-77 0.01010022656828238 78-79 0.014423123539507239 80-81 0.017089583353533788 82-83 0.014786731695965404 84-85 0.012483880038397021 86-87 0.003838086095947304 88-89 0.027028206296723645 90-91 0.029815868829569585 92-93 0.016806777009621878 94-95 0.03102789601776347 96-97 0.02521016551443282 98-99 0.022422502981586885 100-101 0.13542383782753015 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1237596.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.736035627033445 #Duplication Level Percentage of deduplicated Percentage of total 1 81.41247539178295 49.44671005880313 2 11.017145965061163 13.38275539684365 3 3.2246533121235794 5.875579753499073 4 1.3530862511433912 3.287243790235765 5 0.7437156154188795 2.2585169057231083 6 0.4728830990943198 1.7232626852408757 7 0.3286632406363549 1.397319160481014 8 0.22154558983167608 1.0764640669623053 9 0.17783648240670355 0.9720974638115868 >10 0.9567832364031035 10.509701515635307 >50 0.054575279297858846 2.2497838406360224 >100 0.030241813981541157 3.4202672118810207 >500 0.00426314854563812 1.7242902721813569 >1k 0.001998350880767869 2.1900347492661005 >5k 1.3322339205119126E-4 0.48597312879968224 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6006 0.48529568615283175 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4911 0.39681770141467815 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3189 0.25767698021002006 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2102 0.1698454099722365 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2023 0.16346206678108205 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1806 0.1459280734585438 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1769 0.14293840639433222 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1466 0.11845545719281576 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 1451 0.11724343000462185 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1402 0.11328414118985516 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1319 0.10657759074851567 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1301 0.105123158122683 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 8.080181254625904E-5 9 0.0 0.0 0.0 0.0 8.080181254625904E-5 10-11 0.0 0.0 0.0 0.0 8.080181254625904E-5 12-13 2.424054376387771E-4 0.0 0.0 8.080181254625904E-5 8.080181254625904E-5 14-15 2.424054376387771E-4 0.0 0.0 8.080181254625904E-5 8.080181254625904E-5 16-17 3.2320725018503615E-4 0.0 0.0 8.080181254625904E-5 8.080181254625904E-5 18-19 3.6360815645816567E-4 0.0 0.0 8.080181254625904E-5 8.080181254625904E-5 20-21 4.040090627312952E-4 0.0 0.0 8.080181254625904E-5 8.080181254625904E-5 22-23 4.040090627312952E-4 0.0 0.0 8.080181254625904E-5 8.080181254625904E-5 24-25 5.656126878238133E-4 0.0 0.0 2.424054376387771E-4 8.080181254625904E-5 26-27 5.656126878238133E-4 0.0 0.0 6.868154066432019E-4 8.080181254625904E-5 28-29 6.060135940969427E-4 0.0 0.0 0.001939243501110217 8.080181254625904E-5 30-31 6.464145003700723E-4 0.0 0.0 0.0058581314096037805 8.080181254625904E-5 32-33 6.464145003700723E-4 0.0 0.0 0.013776709039137166 8.080181254625904E-5 34-35 9.292208442819789E-4 0.0 0.0 0.026502994515172965 8.080181254625904E-5 36-37 0.0012928290007401446 0.0 0.0 0.04892549749675985 8.080181254625904E-5 38-39 0.0012928290007401446 0.0 0.0 0.08403388504810941 8.080181254625904E-5 40-41 0.0012928290007401446 0.0 0.0 0.12592962485334472 8.080181254625904E-5 42-43 0.001333229907013274 0.0 0.0 0.16927979728441267 8.080181254625904E-5 44-45 0.0015352344383789216 0.0 0.0 0.2199829346571902 8.080181254625904E-5 46-47 0.0018584416885639578 0.0 0.0 0.28268514119308724 8.080181254625904E-5 48-49 0.0018584416885639578 0.0 0.0 0.34878102385592713 8.080181254625904E-5 50-51 0.0018584416885639578 0.0 0.0 0.42012902433427385 8.080181254625904E-5 52-53 0.0018584416885639578 0.0 0.0 0.48897216862368653 8.080181254625904E-5 54-55 0.001939243501110217 0.0 0.0 0.5666227104806415 8.080181254625904E-5 56-57 0.002020045313656476 0.0 0.0 0.6590599840335618 8.080181254625904E-5 58-59 0.002020045313656476 0.0 0.0 0.7559413572765263 8.080181254625904E-5 60-61 0.002020045313656476 0.0 0.0 0.8645389933386987 8.080181254625904E-5 62-63 0.002020045313656476 0.0 0.0 0.9716417958687649 1.2120271881938856E-4 64-65 0.002020045313656476 0.0 0.0 1.0766437512726283 1.6160362509251808E-4 66-67 0.0021008471262027348 0.0 0.0 1.199381704530396 2.020045313656476E-4 68-69 0.0021008471262027348 0.0 0.0 1.3386032275476003 2.424054376387771E-4 70-71 0.002181648938748994 0.0 0.0 1.4847333055375098 2.424054376387771E-4 72-73 0.0023028516575683824 0.0 0.0 1.633206636091261 2.424054376387771E-4 74-75 0.0023836534701146416 0.0 0.0 1.7812759575822805 2.424054376387771E-4 76-77 0.002424054376387771 0.0 0.0 1.947404484177389 2.424054376387771E-4 78-79 0.002424054376387771 0.0 0.0 2.130258985969573 2.424054376387771E-4 80-81 0.0026664598140265484 0.0 0.0 2.3327887291167713 2.424054376387771E-4 82-83 0.0026664598140265484 0.0 0.0 2.537702125734085 2.424054376387771E-4 84-85 0.0027472616265728075 0.0 0.0 2.7504128972621116 2.424054376387771E-4 86-87 0.002868464345392196 0.0 0.0 2.9702746292004822 2.424054376387771E-4 88-89 0.0029088652516653254 0.0 0.0 3.199751776831858 2.424054376387771E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2345 0.0 56.99288 1 GTATCAA 3965 0.0 43.939503 1 TGGTATC 715 0.0 34.56222 2 ATCAACG 5065 0.0 33.96514 3 TCAACGC 5125 0.0 33.660233 4 TATCAAC 5140 0.0 33.654457 2 CAACGCA 5305 0.0 32.60771 5 AACGCAG 5435 0.0 31.913916 6 GTGGTAT 970 0.0 29.032585 1 ACGCAGA 6260 0.0 27.24372 7 CGCAGAG 6275 0.0 27.102888 8 TAAGGTG 800 0.0 26.137678 5 AGGTAAG 775 0.0 25.141228 2 GTAAGGT 860 0.0 24.866714 4 GCAGAGT 7025 0.0 23.735014 9 GAGTACT 4785 0.0 23.281769 12-13 CTAACGA 105 0.0021901305 22.630026 4 TTACGTA 105 0.0021901305 22.630026 4 TACATGG 3810 0.0 22.576569 2 AGGCGAC 575 0.0 22.315172 5 >>END_MODULE