FastQCFastQC Report
Thu 26 May 2016
SRR938210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938210_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237596
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61290.4952343090960216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55330.4470764288184512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35740.2887856780403298No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA17540.14172637920613834No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16790.13566624326516893No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC16290.13162615263785596No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA15740.12718205294781174No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG15440.12475799857142396No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA15010.12128352063193482No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14940.120717907944111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25050.059.7283551
GTATCAA43750.042.0156061
TGGTATC9150.040.490322
GTGGTAT10100.037.1521071
TATCAAC57950.031.6381862
ATCAACG58000.031.6083583
TCAACGC59700.030.9469534
CAACGCA61350.030.1146415
AACGCAG63750.028.9809116
TAAGGTG7750.028.803075
AGGTAAG7550.026.4228742
GTAAGGT8000.026.1219354
ACGCAGA72550.025.400197
CGCAGAG74300.024.674098
GGTAAGG8650.023.0608773
GCAGAGT79800.022.7354249
GTACATG38800.022.4025331
GAGTACT55750.022.36284412-13
AAGGTAA8750.021.7134911
TACATGG38400.021.6463432