##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938210_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1237596 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.651862966590066 31.0 31.0 34.0 30.0 34.0 2 31.96513401788629 31.0 31.0 34.0 30.0 34.0 3 31.943605182951465 31.0 31.0 34.0 30.0 34.0 4 35.699063345388964 37.0 35.0 37.0 33.0 37.0 5 35.58036790681289 37.0 35.0 37.0 33.0 37.0 6 35.682954696039744 37.0 35.0 37.0 33.0 37.0 7 35.62735577684479 37.0 35.0 37.0 33.0 37.0 8 35.611900814159064 37.0 35.0 37.0 33.0 37.0 9 37.13971360605561 39.0 37.0 39.0 33.0 39.0 10-11 37.0434083497361 39.0 37.0 39.0 33.0 39.0 12-13 36.9248248216704 39.0 37.0 39.0 33.0 39.0 14-15 37.85720986493169 39.5 37.0 41.0 33.0 41.0 16-17 37.70283194192612 39.0 37.0 40.0 33.0 41.0 18-19 37.66490195508066 39.0 37.0 40.0 32.5 41.0 20-21 37.62579589785358 39.0 36.5 40.0 32.0 41.0 22-23 37.62587750768425 39.0 37.0 40.5 32.0 41.0 24-25 37.49908774753635 39.0 36.5 40.5 32.0 41.0 26-27 37.19509880445638 39.0 36.0 40.0 31.0 41.0 28-29 37.050193277935605 39.0 36.0 40.0 31.0 41.0 30-31 36.686541084489605 38.5 35.5 40.0 30.5 41.0 32-33 36.35348409335518 38.0 35.0 40.0 30.0 41.0 34-35 36.02682054563848 38.0 35.0 40.0 30.0 41.0 36-37 35.87238808140944 38.0 35.0 40.0 29.5 41.0 38-39 35.727098746279076 38.0 35.0 40.0 29.0 41.0 40-41 35.48660992763389 38.0 34.0 40.0 28.0 41.0 42-43 35.16334167208039 38.0 34.0 40.0 27.0 41.0 44-45 34.73610774436892 38.0 33.0 40.0 26.0 41.0 46-47 34.185518537551836 37.5 33.0 40.0 24.0 41.0 48-49 33.57305655480464 37.0 32.5 39.5 23.0 40.5 50-51 33.04376306969318 36.5 31.5 39.0 21.0 40.0 52-53 32.23482461158569 36.0 30.5 39.0 18.0 40.0 54-55 31.61555830820397 35.0 30.0 38.5 14.0 40.0 56-57 31.107331875668635 35.0 29.0 38.0 10.0 40.0 58-59 30.440903978358044 34.5 28.0 38.0 7.5 40.0 60-61 29.694080297609236 34.0 27.0 38.0 6.0 40.0 62-63 28.95325130333324 33.5 26.0 37.0 2.0 39.0 64-65 28.193313892417237 33.0 25.5 36.5 2.0 39.0 66-67 27.35118326174293 32.0 23.5 36.0 2.0 39.0 68-69 26.50730246380887 31.0 22.0 35.0 2.0 38.0 70-71 25.593423055665987 30.5 19.5 35.0 2.0 38.0 72-73 24.85559908079858 30.0 18.0 34.0 2.0 37.0 74-75 24.070253539927407 29.5 14.0 34.0 2.0 36.0 76-77 23.438770002488695 28.5 15.0 33.0 2.0 35.0 78-79 24.029371458860567 30.0 14.0 34.0 2.0 36.0 80-81 23.61193879101096 30.0 7.5 34.0 2.0 35.5 82-83 22.816928949350192 29.0 4.5 34.0 2.0 35.0 84-85 21.924569487942755 29.0 2.0 34.0 2.0 35.0 86-87 20.93864031557956 27.5 2.0 33.0 2.0 35.0 88-89 19.80605544943584 26.0 2.0 32.5 2.0 34.5 90-91 18.795272851560604 25.0 2.0 32.0 2.0 34.0 92-93 17.677056567732926 22.0 2.0 32.0 2.0 34.0 94-95 16.42330251552203 17.0 2.0 31.0 2.0 34.0 96-97 15.101978351578381 2.0 2.0 31.0 2.0 34.0 98-99 13.709345376035476 2.0 2.0 30.5 2.0 34.0 100-101 11.370207240488819 2.0 2.0 26.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2684532906581545 1101 2 0.28799793402544793 1101 3 0.3852639647875513 1101 4 0.2790883279504399 1101 5 0.2963415907012319 1101 6 0.23421225758708175 1101 7 0.22887031409315028 1101 8 0.27760858429708435 1101 9 0.32968186403432753 1101 10-11 0.4721355055944443 1101 12-13 0.3562947220666146 1101 14-15 0.6187215838286235 1101 16-17 0.47978766674068396 1101 18-19 0.4117935302137212 1101 20-21 0.4028129696697178 1101 22-23 0.440911552215276 1101 24-25 0.543270705535825 1101 26-27 0.7635722853264539 1101 28-29 0.674619848245996 1101 30-31 0.4913327840265609 1101 32-33 0.774656237538629 1101 34-35 0.8385399332572021 1101 36-37 0.9482306607318165 1101 38-39 1.0920722681895114 1101 40-41 1.1757155523581133 1101 42-43 1.3576482642460945 1101 44-45 1.2668735775003555 1101 46-47 1.334402291468244 1101 48-49 1.213493168210654 1101 50-51 1.5080833421816635 1101 52-53 1.593662172833639 1101 54-55 1.8245019159787113 1101 56-57 2.1714860389812323 1101 58-59 1.9266920235024223 1101 60-61 2.030567306651971 1101 62-63 2.0952263662938826 1101 64-65 2.2052474328222083 1101 66-67 2.2420777311119124 1101 68-69 2.6791848664643645 1101 70-71 2.8378204147422537 1101 72-73 2.638897586164905 1101 74-75 2.6998649722710333 1101 76-77 2.893395455988397 1101 78-79 3.315316571697039 1101 80-81 3.397944395244057 1101 82-83 3.434750889134783 1101 84-85 3.557918654819929 1101 86-87 3.939579825686831 1101 88-89 4.287438644372859 1101 90-91 4.6109799446567585 1101 92-93 4.842596992220965 1101 94-95 4.702769866249824 1101 96-97 4.817984598957109 1101 98-99 4.77133671625217 1101 100-101 4.106208576245729 1102 1 0.14884819611863875 1102 2 0.28230405052625684 1102 3 0.3583663505976027 1102 4 0.16186233525082372 1102 5 0.07849589806684776 1102 6 0.036204365029497865 1102 7 0.1251498013082255 1102 8 0.13361104551668035 1102 9 0.21245124498208412 1102 10-11 0.24157948740361945 1102 12-13 0.3221163854254385 1102 14-15 0.3736289873807479 1102 16-17 0.3252246951418627 1102 18-19 0.4682535377084136 1102 20-21 0.45879183649090294 1102 22-23 0.5095012780113493 1102 24-25 0.5368666769079553 1102 26-27 0.4225644112742799 1102 28-29 0.17725397777461893 1102 30-31 0.20698327233807845 1102 32-33 0.28095386005600886 1102 34-35 0.43610450557402913 1102 36-37 0.5731606871482953 1102 38-39 0.6762713077587108 1102 40-41 0.5937840038716189 1102 42-43 0.5228252453659508 1102 44-45 0.5145904724775221 1102 46-47 0.47787184775476277 1102 48-49 0.5560352564537041 1102 50-51 0.868143692951719 1102 52-53 0.8639504639977673 1102 54-55 0.6901179379631337 1102 56-57 1.1003870951775419 1102 58-59 1.2130522637026502 1102 60-61 1.2082082600507213 1102 62-63 1.381429888798774 1102 64-65 1.5064455424945287 1102 66-67 1.5831457477482758 1102 68-69 1.6794398941530844 1102 70-71 2.390191304806237 1102 72-73 2.009840274908523 1102 74-75 2.11840010710608 1102 76-77 2.441403117228351 1102 78-79 2.7891366204587946 1102 80-81 3.067198927913047 1102 82-83 2.8555170825255765 1102 84-85 3.076039499776975 1102 86-87 3.5279714973268277 1102 88-89 3.4115486497856935 1102 90-91 3.697118004271939 1102 92-93 3.9509629413403395 1102 94-95 3.6134390450259666 1102 96-97 3.5719193118687986 1102 98-99 3.366754082654671 1102 100-101 2.629678063136959 1103 1 0.41739227003328594 1103 2 0.5603026831953386 1103 3 0.5967377075469393 1103 4 0.4250313356644 1103 5 0.22687662376507234 1103 6 0.3300187400101535 1103 7 0.3797167147134104 1103 8 0.5180026535934985 1103 9 0.5535257532634361 1103 10-11 0.6988257785888337 1103 12-13 0.5120164239695342 1103 14-15 0.7027829584079583 1103 16-17 0.6764585284231472 1103 18-19 0.710821840952029 1103 20-21 0.6575070799799079 1103 22-23 0.7804191903277413 1103 24-25 0.8888875694993246 1103 26-27 0.8390172566449081 1103 28-29 0.8344773394392675 1103 30-31 0.9152466213985306 1103 32-33 0.8575433822498226 1103 34-35 0.8837175846851011 1103 36-37 0.9500720666613987 1103 38-39 1.1781091862012971 1103 40-41 1.0343177693490446 1103 42-43 1.0896064189635268 1103 44-45 1.145801488775767 1103 46-47 1.2144601883045638 1103 48-49 1.5723060723788862 1103 50-51 1.6873423132416718 1103 52-53 1.4250681866884278 1103 54-55 1.5824279675886395 1103 56-57 1.7647305653283674 1103 58-59 1.7792341749836957 1103 60-61 1.8726790382758374 1103 62-63 1.9462958144058469 1103 64-65 1.9604183984436823 1103 66-67 2.048560856473589 1103 68-69 2.1534985880988486 1103 70-71 2.6029737388240832 1103 72-73 2.443414297871808 1103 74-75 2.463501348676317 1103 76-77 2.0409017625579047 1103 78-79 2.6426240176628006 1103 80-81 2.9019678180398465 1103 82-83 2.957628005136332 1103 84-85 3.201506882182297 1103 86-87 2.919855994438837 1103 88-89 3.2328397646668137 1103 90-91 3.2239124854317502 1103 92-93 3.8966305280012676 1103 94-95 3.896816267993753 1103 96-97 3.3839872732796774 1103 98-99 3.5021824426734636 1103 100-101 3.1936872968408228 1104 1 0.07940333352309281 1104 2 0.1080002721128892 1104 3 0.20571443630185726 1104 4 0.09656553159785375 1104 5 0.07663930716915957 1104 6 0.15763210015585827 1104 7 0.09046333100531712 1104 8 -0.03139305236413037 1104 9 0.030418361579336306 1104 10-11 0.20531491483767894 1104 12-13 0.14937398323097995 1104 14-15 0.28155378708636647 1104 16-17 0.18425341375961324 1104 18-19 0.2703158589488197 1104 20-21 0.381657175109666 1104 22-23 0.2247144514437025 1104 24-25 0.2772742906032377 1104 26-27 0.33614846800902143 1104 28-29 0.2381359420812359 1104 30-31 0.4565810109769117 1104 32-33 0.6960926025920102 1104 34-35 0.875429107912332 1104 36-37 0.7375183234237355 1104 38-39 0.8081018060275653 1104 40-41 0.9380762413145476 1104 42-43 1.0957123278420724 1104 44-45 1.0276794383062153 1104 46-47 1.1945008808221473 1104 48-49 1.063158042343609 1104 50-51 1.11473325255497 1104 52-53 1.1166358303817674 1104 54-55 0.9479256553598923 1104 56-57 1.5655434000598696 1104 58-59 1.539479413417471 1104 60-61 1.6896916929040167 1104 62-63 1.79247911354663 1104 64-65 1.737503297524885 1104 66-67 1.810207650837576 1104 68-69 2.268774532117863 1104 70-71 2.301574214134355 1104 72-73 2.3755217775763278 1104 74-75 2.445907759271268 1104 76-77 2.5228748418507614 1104 78-79 2.6979621176404613 1104 80-81 2.9704270544288427 1104 82-83 3.1230468130690063 1104 84-85 3.4627799282982217 1104 86-87 3.441526283484354 1104 88-89 3.2881907291675 1104 90-91 3.247395656604386 1104 92-93 3.6408570654810397 1104 94-95 3.704657871861233 1104 96-97 3.5997280328282013 1104 98-99 3.6639951216765336 1104 100-101 2.9662428681949162 1105 1 0.11121304679832278 1105 2 0.10933916310695224 1105 3 0.26317464850344763 1105 4 0.15216897987372136 1105 5 0.13863406528460587 1105 6 0.18100111619729375 1105 7 0.204425374708741 1105 8 0.10381914922738389 1105 9 0.06212386871587938 1105 10-11 0.06525554891624807 1105 12-13 0.27482396428440836 1105 14-15 0.016324691469343122 1105 16-17 0.23311977728115352 1105 18-19 0.3829421694199624 1105 20-21 0.33626356010398695 1105 22-23 0.30993296855877617 1105 24-25 0.3832606174947344 1105 26-27 0.30035760657161603 1105 28-29 0.21375249506848348 1105 30-31 0.3452406989900467 1105 32-33 0.3871182436176568 1105 34-35 0.29465040385776575 1105 36-37 0.5380251441891843 1105 38-39 0.5868808574359221 1105 40-41 0.8369986551076494 1105 42-43 0.636299829420949 1105 44-45 0.859186795891155 1105 46-47 0.8859670296158768 1105 48-49 0.9273498132164164 1105 50-51 1.2260909322316138 1105 52-53 1.0200020044376004 1105 54-55 1.25701685152006 1105 56-57 1.6054748766292768 1105 58-59 1.5423245567798567 1105 60-61 1.529153452408881 1105 62-63 1.4891097817290238 1105 64-65 1.691652596501772 1105 66-67 1.755936778032222 1105 68-69 1.9358414450899453 1105 70-71 2.174398516269001 1105 72-73 2.2151114264332215 1105 74-75 1.7456290867943203 1105 76-77 2.1386407569701262 1105 78-79 2.3143951099355213 1105 80-81 2.5928313742469555 1105 82-83 2.895815854372529 1105 84-85 2.9951959318349175 1105 86-87 3.1403176501636523 1105 88-89 3.4368202605556526 1105 90-91 3.2891081108168443 1105 92-93 3.3616856621717055 1105 94-95 3.4049728585986365 1105 96-97 3.3722460320703416 1105 98-99 3.435216070268167 1105 100-101 3.188192470316933 1106 1 -0.03907028907424248 1106 2 0.07821665366947883 1106 3 0.1799714507650556 1106 4 0.09553460376280754 1106 5 -0.0819838566086446 1106 6 0.019801202531148476 1106 7 0.03672291334737565 1106 8 -0.01659753859954094 1106 9 -0.18293838476709823 1106 10-11 -0.00985067558564623 1106 12-13 0.014701954229053626 1106 14-15 -0.013395421780366235 1106 16-17 0.04901494089228464 1106 18-19 -0.016378374652624927 1106 20-21 0.06284179804686829 1106 22-23 0.13777098254147546 1106 24-25 0.06257318414955648 1106 26-27 0.0395452430552794 1106 28-29 0.061448883435417656 1106 30-31 0.2320105642415129 1106 32-33 0.344408376165525 1106 34-35 0.020648321688248927 1106 36-37 0.12840801792167866 1106 38-39 0.1945109295500842 1106 40-41 0.350471921235993 1106 42-43 0.49321997483426117 1106 44-45 0.6851357277212671 1106 46-47 -0.032975422942818966 1106 48-49 0.3356534163853766 1106 50-51 0.6958555666669781 1106 52-53 0.4141076025958057 1106 54-55 0.5415059582185471 1106 56-57 0.5532270222795823 1106 58-59 0.8903706184323887 1106 60-61 0.9084579781651492 1106 62-63 1.0517801218167087 1106 64-65 0.9064546211153832 1106 66-67 0.984396692036924 1106 68-69 0.734385725048643 1106 70-71 1.1282310624406833 1106 72-73 1.1471504925040144 1106 74-75 1.0529146698864267 1106 76-77 1.4429363954174725 1106 78-79 1.6521424733672063 1106 80-81 1.8582466231762496 1106 82-83 1.8289906973634054 1106 84-85 2.110918216127235 1106 86-87 2.369197066309777 1106 88-89 2.990905883429047 1106 90-91 2.6777004034039784 1106 92-93 2.363031454665741 1106 94-95 2.3331046827822703 1106 96-97 2.4618914707648383 1106 98-99 2.6222595376729085 1106 100-101 2.115695305791684 1107 1 0.0071017592943221075 1107 2 0.011034996132625707 1107 3 0.1298016267701989 1107 4 0.10455982139086473 1107 5 0.09690740452504798 1107 6 0.04416997341750317 1107 7 -0.07643158706733288 1107 8 0.00783167722462963 1107 9 0.02864254995341753 1107 10-11 0.03023745838977021 1107 12-13 0.11677952558299154 1107 14-15 0.26911134016854277 1107 16-17 0.16309897932895723 1107 18-19 0.1255628959945696 1107 20-21 0.20553258592298107 1107 22-23 0.1790320111145789 1107 24-25 0.16524285309000675 1107 26-27 0.2850460372492094 1107 28-29 0.23486041675003833 1107 30-31 0.15906902973902248 1107 32-33 0.37908826503093707 1107 34-35 0.5503575342326243 1107 36-37 0.5528221487918046 1107 38-39 0.3782283247622189 1107 40-41 0.5707671763609383 1107 42-43 0.5665643879874978 1107 44-45 0.5434617288208372 1107 46-47 0.8652321481272907 1107 48-49 0.7712997935260475 1107 50-51 0.7314450204444682 1107 52-53 0.7499793605133078 1107 54-55 1.0207355007607468 1107 56-57 0.9527283778230107 1107 58-59 1.1047522562063712 1107 60-61 1.2808463626454092 1107 62-63 1.1983414881456227 1107 64-65 1.1714084359268107 1107 66-67 1.4498269180799674 1107 68-69 1.5412533737615846 1107 70-71 1.9715446434195556 1107 72-73 2.1695582193643794 1107 74-75 1.9501543335392562 1107 76-77 1.9417624496034662 1107 78-79 1.8970577400765514 1107 80-81 2.2913995152299975 1107 82-83 2.7973932375224955 1107 84-85 3.1232587071924236 1107 86-87 3.171569188473729 1107 88-89 3.170434181646499 1107 90-91 3.4096024006098453 1107 92-93 3.4315003265739783 1107 94-95 3.147045177631835 1107 96-97 2.7548268241154314 1107 98-99 2.767173385240236 1107 100-101 2.389111149093175 1108 1 -0.05636580427650273 1108 2 -0.021156865166076955 1108 3 0.12878764059333392 1108 4 0.10149664109749068 1108 5 -0.08737772486817619 1108 6 0.020829140002646795 1108 7 0.032420527784850606 1108 8 -0.06851024988741727 1108 9 -0.10484130261433933 1108 10-11 0.05838942070406716 1108 12-13 0.034993439916426894 1108 14-15 0.05354947329524862 1108 16-17 0.1239254168834023 1108 18-19 -0.002953511274860432 1108 20-21 0.06904119548860876 1108 22-23 0.18420572159487136 1108 24-25 0.14393723848446172 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-0.9502564204314652 2316 50-51 -0.8218143430719067 2316 52-53 -1.055438216628346 2316 54-55 -1.0981401288133092 2316 56-57 -1.2750756569274664 2316 58-59 -1.3780130685251386 2316 60-61 -1.3815851121323774 2316 62-63 -1.5069840831032657 2316 64-65 -1.683615651727056 2316 66-67 -1.8485606056288901 2316 68-69 -2.1009002943344584 2316 70-71 -2.0792320214033424 2316 72-73 -2.1405682704830262 2316 74-75 -2.062671726034562 2316 76-77 -2.17172152512412 2316 78-79 -2.394088883361949 2316 80-81 -2.7568871488444415 2316 82-83 -2.941076361284349 2316 84-85 -3.150119278207118 2316 86-87 -3.3398439234612987 2316 88-89 -3.4911128317217397 2316 90-91 -3.619619510666535 2316 92-93 -3.9179833268631867 2316 94-95 -3.642473087545941 2316 96-97 -3.297599192376259 2316 98-99 -3.473925866116579 2316 100-101 -3.2130738083084616 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 35.0 5 48.0 6 108.0 7 302.0 8 717.0 9 1296.0 10 2150.0 11 3320.0 12 5101.0 13 7394.0 14 10231.0 15 13650.0 16 17707.0 17 22451.0 18 23247.0 19 22405.0 20 23019.0 21 24331.0 22 25865.0 23 28277.0 24 31015.0 25 34928.0 26 39665.0 27 46760.0 28 54564.0 29 61957.0 30 71323.0 31 80512.0 32 90636.0 33 100083.0 34 109625.0 35 114438.0 36 97636.0 37 59041.0 38 13455.0 39 289.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6270770105915 19.78230375663787 15.386119541433555 22.204499691337077 2 17.621048191398327 21.12358515494508 37.533565575194565 23.721801078462033 3 21.06924794993875 23.683904312158795 29.944227344277767 25.302620393624693 4 14.956738709562734 17.568010885620186 34.80578476336381 32.66946564145327 5 14.220968895317126 37.042004856192854 33.963938121921956 14.773088126568062 6 33.65772620099968 35.82451110784312 15.874430548305826 14.64333214285137 7 29.347864731301655 32.74283368724527 19.504183917853645 18.40511766359943 8 27.317822066184817 35.01969545772244 18.492479365220447 19.170003110872297 9 26.400295411426665 16.517021709830995 19.3874252987243 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23.001612205893643 25.96479815101399 28.97962317212621 22.053966470966152 36-37 23.051276280856534 25.46666865989803 29.111979064606153 22.370075994639286 38-39 22.471688095059143 25.92124308113049 29.04579606700302 22.561272756807345 40-41 22.904312540484494 25.72536694154029 29.0890297177895 22.28129080018572 42-43 22.712430383235162 25.76180934290017 28.86484922613917 22.66091104772549 44-45 22.76044640162328 26.003407689159275 29.043499552119584 22.192646357097864 46-47 23.34428216471729 26.31181948568774 28.192311577076133 22.151586772518836 48-49 23.04481676154154 26.525842460578787 27.983104327344567 22.44623645053511 50-51 23.108267964666986 26.648558980475052 28.015119635163654 22.22805341969431 52-53 23.457008587616638 26.375691259506333 27.95467987937905 22.212620273497976 54-55 22.98597442137822 26.60326956454287 27.985788577209362 22.424967436869544 56-57 23.027466152120724 26.787618899867162 27.734171732940315 22.4507432150718 58-59 22.96545076099147 26.36995433081555 27.85020313575674 22.81439177243624 60-61 22.706319348155617 26.645246106160652 28.04239024689802 22.60604429878571 62-63 22.880002844223803 26.656437157198308 28.130545024385988 22.3330149741919 64-65 22.631779676081692 26.647225750568037 28.018270905852958 22.70272366749731 66-67 22.498502133374167 26.760846756224883 27.95376672550397 22.78688438489698 68-69 23.067096317532243 26.273034613936396 28.095684045009918 22.564185023521446 70-71 22.845217663922636 26.54412263775901 27.60549484646039 23.005164851857955 72-73 22.823603179066513 26.754328553098105 27.552206051086138 22.869862216749244 74-75 22.938422554694746 26.996693589830606 27.240917068251676 22.82396678722297 76-77 22.91701007436999 26.713725642293607 27.65583437567671 22.713429907659688 78-79 22.92565586831244 26.925062783008347 27.587799249512766 22.56148209916645 80-81 23.13198962341027 26.850698553281028 27.27700977744308 22.740302045865626 82-83 23.142292218648063 27.003636916030953 27.222692222849787 22.631378642471194 84-85 23.010395930997618 27.1158412397383 27.26638042942051 22.607382399843562 86-87 23.35553918857389 26.937591837015027 26.967973551747697 22.738895422663386 88-89 23.038293595001925 27.204071441730584 26.69211115743748 23.06552380583001 90-91 23.073414288854124 27.14730370925828 26.86428529871876 22.914996703168836 92-93 23.231938810180903 26.69608671358845 26.99186282388009 23.080111652350553 94-95 23.037535285155773 26.460341848366447 27.12833070255992 23.373792163917855 96-97 23.325087498368813 26.133640703800804 27.044076230138387 23.497195567692003 98-99 23.016163197102767 26.51391347172804 26.600290644237152 23.86963268693204 100-101 23.77956497041382 25.910068998061526 26.6174627556722 23.692903275852455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 565.0 1 412.0 2 361.5 3 481.5 4 402.5 5 337.0 6 718.0 7 1671.0 8 2569.0 9 2853.0 10 2260.0 11 1491.5 12 1336.5 13 1388.0 14 1449.0 15 1575.0 16 1667.0 17 1778.5 18 1980.5 19 2197.5 20 2481.0 21 2857.5 22 3442.5 23 4254.0 24 5201.5 25 6411.5 26 7914.0 27 9387.0 28 10625.5 29 11865.0 30 13471.0 31 16034.0 32 19714.5 33 21039.0 34 20555.0 35 21549.0 36 23512.5 37 25861.0 38 27817.0 39 29114.5 40 30846.5 41 33180.5 42 34525.0 43 35708.0 44 37466.0 45 39847.0 46 41768.0 47 43144.5 48 43629.5 49 43646.5 50 43689.5 51 43204.0 52 43751.0 53 45025.0 54 46243.5 55 44992.0 56 41945.5 57 38859.5 58 35862.0 59 31929.0 60 26483.0 61 22245.0 62 18919.0 63 15020.0 64 11143.5 65 8187.0 66 6244.0 67 4882.0 68 3804.5 69 3042.0 70 2376.5 71 1656.5 72 1175.0 73 893.0 74 641.0 75 436.0 76 297.5 77 200.0 78 140.0 79 82.5 80 48.5 81 37.0 82 23.0 83 14.0 84 9.5 85 7.0 86 4.0 87 1.5 88 0.5 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.007514568566802091 3 0.005817730503330651 4 0.0 5 8.080181254625904E-5 6 1.6160362509251808E-4 7 0.0 8 8.080181254625904E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010504235631013674 26-27 0.01854401597936645 28-29 2.020045313656476E-4 30-31 0.0 32-33 0.003030067970484714 34-35 0.013009091819947706 36-37 0.006019735034696298 38-39 0.017695596947630732 40-41 0.01935203410482904 42-43 0.0158775561653399 44-45 0.008120582160899033 46-47 0.00169683806347144 48-49 8.080181254625904E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.020045313656476E-4 68-69 1.6160362509251808E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.020045313656476E-4 82-83 7.272163129163313E-4 84-85 0.0035956806583085275 86-87 7.676172191894609E-4 88-89 0.0 90-91 0.0035552797520353977 92-93 3.6360815645816567E-4 94-95 4.040090627312952E-5 96-97 1.2120271881938856E-4 98-99 4.040090627312952E-5 100-101 0.002020045313656476 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1237596.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.88940661932263 #Duplication Level Percentage of deduplicated Percentage of total 1 81.95547646928111 50.72175807887667 2 10.767444932672007 13.327815553786056 3 3.071503519720025 5.702805907938998 4 1.3666896260001662 3.3833443994373704 5 0.7161492709966436 2.2161026716421377 6 0.44426266950679805 1.649709179933518 7 0.3193242086359487 1.383395005416455 8 0.20779177966261114 1.0288087954953629 9 0.16165957272016482 0.9004513526985813 >10 0.9043730008674292 10.07391486748951 >50 0.051725942216100836 2.1954868668053815 >100 0.02850033689067618 3.5341257254535257 >500 0.0033991138869041895 1.4710466774869404 >1k 0.0014380866444594648 1.4676484067397622 >5k 2.6147029899263E-4 0.9435865107998845 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6129 0.4952343090960216 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5533 0.4470764288184512 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3574 0.2887856780403298 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1754 0.14172637920613834 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1679 0.13566624326516893 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1629 0.13162615263785596 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1574 0.12718205294781174 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1544 0.12475799857142396 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1501 0.12128352063193482 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1494 0.120717907944111 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 8.080181254625904E-5 0.0 0.0 0.0 0.0 6 8.080181254625904E-5 0.0 0.0 0.0 0.0 7 1.6160362509251808E-4 0.0 0.0 0.0 0.0 8 1.6160362509251808E-4 0.0 0.0 0.0 0.0 9 1.6160362509251808E-4 0.0 0.0 0.0 0.0 10-11 1.6160362509251808E-4 0.0 0.0 0.0 0.0 12-13 3.6360815645816567E-4 0.0 0.0 0.0 0.0 14-15 4.848108752775542E-4 0.0 0.0 0.0 0.0 16-17 4.848108752775542E-4 0.0 0.0 0.0 0.0 18-19 4.848108752775542E-4 0.0 0.0 0.0 8.080181254625904E-5 20-21 4.848108752775542E-4 0.0 0.0 0.0 8.080181254625904E-5 22-23 4.848108752775542E-4 0.0 0.0 0.0 8.080181254625904E-5 24-25 6.464145003700723E-4 0.0 0.0 1.6160362509251808E-4 8.080181254625904E-5 26-27 6.464145003700723E-4 0.0 0.0 5.252117815506838E-4 8.080181254625904E-5 28-29 6.464145003700723E-4 0.0 0.0 0.0017776398760176988 8.080181254625904E-5 30-31 6.464145003700723E-4 0.0 0.0 0.0056157259719650035 8.080181254625904E-5 32-33 6.464145003700723E-4 0.0 0.0 0.013534303601498389 8.080181254625904E-5 34-35 7.272163129163313E-4 0.0 0.0 0.026139386358714798 8.080181254625904E-5 36-37 9.696217505551085E-4 0.0 0.0 0.048036677558751 8.080181254625904E-5 38-39 0.0012928290007401446 0.0 0.0 0.08225624517209171 8.080181254625904E-5 40-41 0.0012928290007401446 0.0 0.0 0.12342476866441068 8.080181254625904E-5 42-43 0.0016160362509251808 0.0 0.0 0.1658861211574698 8.080181254625904E-5 44-45 0.0017776398760176988 0.0 0.0 0.2155792358734191 8.080181254625904E-5 46-47 0.002100847126202735 0.0 0.0 0.27626139709565967 8.080181254625904E-5 48-49 0.002181648938748994 0.0 0.0 0.34021603172602366 8.080181254625904E-5 50-51 0.002181648938748994 0.0 0.0 0.40841276151506634 8.080181254625904E-5 52-53 0.002181648938748994 0.0 0.0 0.4742258378339943 8.080181254625904E-5 54-55 0.002262450751295253 0.0 0.0 0.5486443071890988 8.080181254625904E-5 56-57 0.002585658001480289 0.0 0.0 0.6359910665516049 8.080181254625904E-5 58-59 0.002585658001480289 0.0 0.0 0.7257214793842255 8.080181254625904E-5 60-61 0.002585658001480289 0.0 0.0 0.8251481097223974 8.080181254625904E-5 62-63 0.002585658001480289 0.0 0.0 0.9211406630273531 8.080181254625904E-5 64-65 0.002585658001480289 0.0 0.0 1.0168100090821237 8.080181254625904E-5 66-67 0.002828063439119066 0.0 0.0 1.1280741049583223 8.080181254625904E-5 68-69 0.0029088652516653254 0.0 0.0 1.253316914405024 8.080181254625904E-5 70-71 0.003110869783030973 0.0 0.0 1.3828422199166772 8.080181254625904E-5 72-73 0.0031512706893041024 0.0 0.0 1.5158016024615464 8.080181254625904E-5 74-75 0.0031512706893041024 0.0 0.0 1.6507002285075258 8.080181254625904E-5 76-77 0.0031512706893041024 0.0 0.0 1.8031328478760436 8.080181254625904E-5 78-79 0.0031512706893041024 0.0 0.0 1.9695037799087909 8.080181254625904E-5 80-81 0.0037976851896741747 0.0 0.0 2.1511058536065084 8.080181254625904E-5 82-83 0.0039592888147666925 0.0 0.0 2.3316171028348505 8.080181254625904E-5 84-85 0.00420169425240547 0.0 0.0 2.516410848128145 8.080181254625904E-5 86-87 0.004363297877497988 8.080181254625904E-5 0.0 2.7052042831424794 8.080181254625904E-5 88-89 0.004363297877497988 8.080181254625904E-5 0.0 2.8983206151280383 8.080181254625904E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2505 0.0 59.728355 1 GTATCAA 4375 0.0 42.015606 1 TGGTATC 915 0.0 40.49032 2 GTGGTAT 1010 0.0 37.152107 1 TATCAAC 5795 0.0 31.638186 2 ATCAACG 5800 0.0 31.608358 3 TCAACGC 5970 0.0 30.946953 4 CAACGCA 6135 0.0 30.114641 5 AACGCAG 6375 0.0 28.980911 6 TAAGGTG 775 0.0 28.80307 5 AGGTAAG 755 0.0 26.422874 2 GTAAGGT 800 0.0 26.121935 4 ACGCAGA 7255 0.0 25.40019 7 CGCAGAG 7430 0.0 24.67409 8 GGTAAGG 865 0.0 23.060877 3 GCAGAGT 7980 0.0 22.735424 9 GTACATG 3880 0.0 22.402533 1 GAGTACT 5575 0.0 22.362844 12-13 AAGGTAA 875 0.0 21.713491 1 TACATGG 3840 0.0 21.646343 2 >>END_MODULE