FastQCFastQC Report
Thu 26 May 2016
SRR938209_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938209_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253367
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61330.48932196236218123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50850.40570718712077153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32410.2585834795395124No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA21550.17193687084469275No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20460.16324029593885908No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA18610.14848005412620566No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA17660.14090047049268092No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA14740.11760322395595224No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC13650.1089066490501186No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13500.10770987268693048No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG13380.10675245159638No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA13370.10667266650550078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25500.054.834551
GTATCAA43450.043.1647871
TGGTATC8850.040.8023152
GTGGTAT10400.035.792181
ATCAACG55800.033.2946433
TATCAAC56450.033.162462
TCAACGC57600.032.419174
CAACGCA58600.031.7037755
AACGCAG60100.030.9124976
ACGCAGA68650.027.0754857
CGCAGAG68650.026.866678
TAAGGTG7050.024.263045
GTACATG41750.024.1186391
TACATGG40600.023.7566092
GCAGAGT77550.023.5994369
GTATAGG2252.6648195E-923.3311941
GAGTACT50350.023.12703112-13
ACATGGG41600.022.3869323
CAGAGTA70550.022.26703610-11
GTACTTT53850.021.61179214-15
Produced by FastQC (version 0.11.5)