FastQCFastQC Report
Thu 26 May 2016
SRR938209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938209_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253367
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63830.5092682350819832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56270.4489507063773021No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36250.28922095443712814No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA17680.14106004067443934No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17530.1398632643112512No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA17310.13810799231190865No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC16940.13515594394937794No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG15750.12566151813475224No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15260.12175204868167105No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA15230.12151269340903344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26900.061.1081281
GTATCAA46200.042.0587651
TGGTATC7950.040.0389632
GTGGTAT9900.034.0720561
ATCAACG61450.031.3866253
TCAACGC61450.031.3816184
TATCAAC61850.031.3397542
CAACGCA63500.030.293715
AACGCAG65850.029.2126166
TAAGGTG7700.026.5246055
GTAAGGT7650.026.0770844
ACGCAGA74350.025.8729067
CGCAGAG75550.025.5248228
AGGTAAG8150.023.9001922
GGTAAGG9100.023.4915453
CCTATAC2853.6379788E-1223.3358523
GCAGAGT82750.022.7299429
GAGTACT57750.022.41315512-13
CAGAGTA79200.022.01003510-11
GTACTTT59850.021.54736514-15
Produced by FastQC (version 0.11.5)