##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938209_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1253367 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.687499351746137 31.0 31.0 34.0 30.0 34.0 2 31.989631927440247 31.0 31.0 34.0 30.0 34.0 3 32.042479178085905 33.0 31.0 34.0 30.0 34.0 4 35.735268281357335 37.0 35.0 37.0 33.0 37.0 5 35.632078234068715 37.0 35.0 37.0 33.0 37.0 6 35.71187768626428 37.0 35.0 37.0 33.0 37.0 7 35.65855970358243 37.0 35.0 37.0 33.0 37.0 8 35.64839747655714 37.0 35.0 37.0 33.0 37.0 9 37.185602461210486 39.0 37.0 39.0 33.0 39.0 10-11 37.11732876324332 39.0 37.0 39.0 33.0 39.0 12-13 37.02312371396407 39.0 37.0 39.0 33.0 39.0 14-15 38.02961463003254 40.0 37.0 41.0 33.0 41.0 16-17 37.907655140114585 40.0 37.0 41.0 33.0 41.0 18-19 37.924706011886386 40.0 37.0 41.0 33.0 41.0 20-21 37.78845382078833 39.5 37.0 41.0 32.5 41.0 22-23 37.54879496587991 39.0 36.5 40.0 32.0 41.0 24-25 37.45540133097489 39.0 36.5 40.5 32.0 41.0 26-27 37.35682126623726 39.0 36.0 40.0 32.0 41.0 28-29 37.25406724447029 39.0 36.0 40.0 31.5 41.0 30-31 36.934007357781084 39.0 36.0 40.0 31.0 41.0 32-33 36.65548279155267 38.0 35.0 40.0 30.0 41.0 34-35 36.352544785366135 38.0 35.0 40.0 30.0 41.0 36-37 36.14555991980002 38.0 35.0 40.0 30.0 41.0 38-39 36.090687324622394 38.0 35.0 40.0 30.0 41.0 40-41 35.95978073461325 38.0 35.0 40.0 29.5 41.0 42-43 35.702328208736944 38.0 35.0 40.0 29.0 41.0 44-45 35.37763001578947 38.0 34.0 40.0 27.5 41.0 46-47 34.90881800781415 38.0 34.0 40.0 26.5 41.0 48-49 34.3733495456638 38.0 33.0 40.0 24.0 41.0 50-51 33.89874793256883 37.0 33.0 40.0 23.5 41.0 52-53 33.2152138998394 37.0 32.0 39.0 22.0 40.0 54-55 32.80516560592388 36.0 31.0 39.0 20.0 40.0 56-57 32.41661939400032 36.0 31.0 39.0 18.5 40.0 58-59 31.894576768017668 35.0 30.5 39.0 16.5 40.0 60-61 31.302746123042972 35.0 29.0 38.0 13.0 40.0 62-63 30.716636468009767 34.5 28.5 38.0 8.0 40.0 64-65 30.120803005025664 34.0 28.0 37.5 7.5 39.5 66-67 29.503668917404077 34.0 27.5 37.0 6.5 39.0 68-69 28.862312475117022 33.0 26.0 36.0 2.0 39.0 70-71 28.119963665869612 32.5 26.0 35.5 2.0 38.5 72-73 27.51902196244197 32.0 25.0 35.0 2.0 38.0 74-75 26.929662660657254 31.5 24.0 35.0 2.0 37.0 76-77 26.11807874309759 30.5 23.0 34.0 2.0 36.0 78-79 26.90441387079762 32.0 25.0 34.5 2.0 36.0 80-81 26.669893574667277 32.0 24.5 35.0 2.0 36.0 82-83 26.08871663287768 31.5 23.5 34.0 2.0 36.0 84-85 25.44727083128884 31.0 20.5 34.0 2.0 35.0 86-87 24.68664285879555 31.0 18.5 34.0 2.0 35.0 88-89 23.778855674355555 30.0 13.5 34.0 2.0 35.0 90-91 23.039586569616084 29.5 7.0 34.0 2.0 35.0 92-93 22.17827898771868 29.0 2.0 34.0 2.0 35.0 94-95 21.20358163251466 29.0 2.0 33.5 2.0 35.0 96-97 20.00447035864196 27.0 2.0 33.0 2.0 34.5 98-99 18.675205267092558 25.0 2.0 32.5 2.0 34.0 100-101 16.28541081742219 19.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.027416339098373044 1101 2 0.08073846568990106 1101 3 0.22190127521858471 1101 4 0.06746177436234291 1101 5 0.212620874953366 1101 6 0.24792820804861293 1101 7 0.210604601748976 1101 8 0.1816056829861381 1101 9 0.1154557883035352 1101 10-11 0.18355608105392207 1101 12-13 0.20323041691518995 1101 14-15 0.19798395991398365 1101 16-17 0.29147744301879186 1101 18-19 0.2816045831245333 1101 20-21 0.18855684731259004 1101 22-23 0.31069417275080724 1101 24-25 0.2953473247076843 1101 26-27 0.21557782663710867 1101 28-29 0.35137715351943655 1101 30-31 0.34872894003712673 1101 32-33 0.4280310363270914 1101 34-35 0.2499356139495461 1101 36-37 0.39297993202585246 1101 38-39 0.5100080837660315 1101 40-41 0.5274386266205937 1101 42-43 0.6416474514156292 1101 44-45 0.42504643528516084 1101 46-47 0.36713483525555546 1101 48-49 0.2801492686347373 1101 50-51 -0.0023204669498113617 1101 52-53 0.08084745968788098 1101 54-55 0.20299776841611106 1101 56-57 0.31272505815868357 1101 58-59 0.2338039023532481 1101 60-61 0.17176571741355318 1101 62-63 0.15216235715282522 1101 64-65 0.5037571183890144 1101 66-67 0.4105275818113405 1101 68-69 0.37571397486778935 1101 70-71 0.4759606371739018 1101 72-73 0.44430091906709634 1101 74-75 0.27893330868395694 1101 76-77 0.3652766967149681 1101 78-79 0.6665879775760573 1101 80-81 0.7808034996229729 1101 82-83 0.9159181433834114 1101 84-85 1.0238802102827513 1101 86-87 1.099960418375506 1101 88-89 0.9765767123705551 1101 90-91 1.0852612774397414 1101 92-93 1.3997235605017693 1101 94-95 1.0482264294445578 1101 96-97 1.085620317459263 1101 98-99 1.386251319388439 1101 100-101 1.528689945216744 1102 1 0.23388634289396393 1102 2 0.21330551549833032 1102 3 0.17898939782394763 1102 4 0.07359204256157881 1102 5 0.13062853778861694 1102 6 0.15214276743559196 1102 7 0.1730567090286712 1102 8 0.1747091312619986 1102 9 0.1338465929012358 1102 10-11 0.2283836672608146 1102 12-13 0.09441815637879358 1102 14-15 0.30717936221283537 1102 16-17 0.39032801773143433 1102 18-19 0.3394589892547941 1102 20-21 0.22342274769572867 1102 22-23 0.39536850225272957 1102 24-25 0.2214009645544266 1102 26-27 0.08186135154132756 1102 28-29 0.1360514830213546 1102 30-31 0.33570212011375133 1102 32-33 0.12726475280219063 1102 34-35 0.26564442621008055 1102 36-37 0.297960774937728 1102 38-39 0.34487398414917436 1102 40-41 0.4741819216397474 1102 42-43 0.3991187157834517 1102 44-45 0.38519969199013815 1102 46-47 0.2391654865965478 1102 48-49 0.29624122265772 1102 50-51 0.3321622916708762 1102 52-53 0.1563263868909459 1102 54-55 0.27809355385672063 1102 56-57 0.49854881294795206 1102 58-59 0.5299724847287273 1102 60-61 0.442263801704744 1102 62-63 0.5395186789919109 1102 64-65 0.7194659306495517 1102 66-67 0.5676157044167027 1102 68-69 0.7941047794654885 1102 70-71 0.7227039321930597 1102 72-73 0.5025384669598125 1102 74-75 0.8639907799483204 1102 76-77 0.6396061986306769 1102 78-79 0.6026033032465534 1102 80-81 0.5631789785501731 1102 82-83 0.7201327027703819 1102 84-85 0.7484012830796871 1102 86-87 0.6654776597548171 1102 88-89 0.4440096625621237 1102 90-91 0.6599739211179063 1102 92-93 0.7066201122259059 1102 94-95 0.6191076554982011 1102 96-97 0.9756586316355076 1102 98-99 0.7988949975493576 1102 100-101 0.9742838149485458 1103 1 0.11633070763581443 1103 2 0.15704345336446934 1103 3 0.19234983003973838 1103 4 0.14162447013184476 1103 5 0.08295670753237516 1103 6 0.06665831798126476 1103 7 0.0935898055314297 1103 8 -0.006144028091952691 1103 9 -0.0554220219245849 1103 10-11 0.07827891527821862 1103 12-13 0.011501838410978849 1103 14-15 0.18212655924333632 1103 16-17 0.2742431800116094 1103 18-19 0.21113325613325173 1103 20-21 0.16395237144255503 1103 22-23 0.2344284670697263 1103 24-25 0.16257959514998532 1103 26-27 0.17909367891404315 1103 28-29 0.31466727665994654 1103 30-31 0.32556252671084707 1103 32-33 0.39436693722260685 1103 34-35 0.42357369584964744 1103 36-37 0.46731683489684883 1103 38-39 0.2401661226267393 1103 40-41 0.2539505563912172 1103 42-43 0.34963108961139255 1103 44-45 0.2518367548356295 1103 46-47 0.4760390384288513 1103 48-49 0.54549372617317 1103 50-51 0.574825383106834 1103 52-53 0.4541533525562116 1103 54-55 0.8955151179632281 1103 56-57 0.8715820090789563 1103 58-59 0.8019965890708072 1103 60-61 0.8532596287045671 1103 62-63 0.915302468622599 1103 64-65 0.6334312486344409 1103 66-67 0.6332724253380633 1103 68-69 0.6039304015727147 1103 70-71 0.803051264717979 1103 72-73 0.5850770515555794 1103 74-75 0.3663508307302621 1103 76-77 0.44866923769064115 1103 78-79 0.636109705072883 1103 80-81 0.5592573262015108 1103 82-83 0.6742928936011694 1103 84-85 0.8371589473381995 1103 86-87 1.017512353156011 1103 88-89 1.0105080998219478 1103 90-91 0.755715525303426 1103 92-93 1.0707926569589858 1103 94-95 0.7503738450879034 1103 96-97 1.0296756424465947 1103 98-99 0.7615107798018634 1103 100-101 1.0330317441043242 1104 1 0.12228744212229259 1104 2 0.07217208517426243 1104 3 0.1130260557841325 1104 4 0.06359575770375869 1104 5 0.14989793097260673 1104 6 -0.013910475373059228 1104 7 0.08341628374414967 1104 8 -0.0025706473041822164 1104 9 -0.05587295640380319 1104 10-11 0.06761953908556961 1104 12-13 0.1604966394663876 1104 14-15 0.3756874348481176 1104 16-17 0.1507401999946154 1104 18-19 0.2299300749554689 1104 20-21 0.22782476578365163 1104 22-23 0.36888833005163235 1104 24-25 0.14410530446441783 1104 26-27 0.2948334369272061 1104 28-29 0.19194820774066557 1104 30-31 0.32875508783171625 1104 32-33 0.37425250984890823 1104 34-35 0.24313803815071822 1104 36-37 0.47842962020914825 1104 38-39 0.03690713115820188 1104 40-41 0.20442970177139586 1104 42-43 0.28180586961585874 1104 44-45 0.19369205326076866 1104 46-47 0.22080984823018923 1104 48-49 0.28154060356188637 1104 50-51 0.4224969664257756 1104 52-53 0.3851136340273129 1104 54-55 0.48516281715254195 1104 56-57 0.5549248193686225 1104 58-59 0.5119035645872678 1104 60-61 0.5881307769267288 1104 62-63 0.5292016440724083 1104 64-65 0.607057355761075 1104 66-67 0.5657598349174542 1104 68-69 0.5658847296088503 1104 70-71 0.5343391335906311 1104 72-73 0.8294590678486102 1104 74-75 0.6792066977727806 1104 76-77 0.43783058754651094 1104 78-79 0.7171360319475859 1104 80-81 1.00597089372015 1104 82-83 1.0949911161494335 1104 84-85 1.1590098574009637 1104 86-87 1.0306106249771148 1104 88-89 0.9914508938339779 1104 90-91 1.269151173103804 1104 92-93 1.1688632073080214 1104 94-95 1.236933248006661 1104 96-97 1.2756535268599904 1104 98-99 1.1933330610244042 1104 100-101 1.2066489425253941 1105 1 0.17905800868796717 1105 2 0.10216780886358023 1105 3 0.13621714692842346 1105 4 0.060869408939851155 1105 5 0.07546443509664869 1105 6 0.05546637678059341 1105 7 0.0453549778262996 1105 8 -0.057102439453004195 1105 9 -0.07965063534653183 1105 10-11 -6.553201146175525E-4 1105 12-13 0.08197228242495669 1105 14-15 0.25848628286674824 1105 16-17 0.15326798664791852 1105 18-19 0.283453803127685 1105 20-21 0.2754186586339742 1105 22-23 0.09455068990076398 1105 24-25 0.05817260042847039 1105 26-27 0.26766682190809377 1105 28-29 0.07846220101738055 1105 30-31 0.17712963484517985 1105 32-33 0.2332736789248102 1105 34-35 0.19372015696364286 1105 36-37 0.1989863648181327 1105 38-39 0.21532053961330888 1105 40-41 0.3449268456347312 1105 42-43 0.32397971261691794 1105 44-45 0.3276893654469788 1105 46-47 0.38859991616411804 1105 48-49 0.4467627442541158 1105 50-51 0.410698041872422 1105 52-53 0.4981029678701674 1105 54-55 0.28159169632562 1105 56-57 0.3544825236052773 1105 58-59 0.46014843073981737 1105 60-61 0.20134409525283203 1105 62-63 0.3678661827526817 1105 64-65 0.521811323390633 1105 66-67 0.2979503924595228 1105 68-69 0.4952841247169424 1105 70-71 0.5814152128506329 1105 72-73 0.4408144121397939 1105 74-75 0.38453249752048535 1105 76-77 0.44103487691626597 1105 78-79 0.4616719612530531 1105 80-81 0.5836060029266363 1105 82-83 0.8135469862288005 1105 84-85 1.0676522866451528 1105 86-87 0.9817557824432903 1105 88-89 0.848018937750993 1105 90-91 0.721023945635654 1105 92-93 0.8398096635095555 1105 94-95 0.6278351428029936 1105 96-97 0.7853801266363831 1105 98-99 0.9548586281707792 1105 100-101 0.9609569642741 1106 1 -0.07445948419249149 1106 2 0.040541897307431185 1106 3 0.07750680578663349 1106 4 -0.022093448026460294 1106 5 -0.151263849351146 1106 6 -0.018621850255563288 1106 7 0.09957678232535017 1106 8 -0.11063153173983409 1106 9 -0.0880591209085253 1106 10-11 -0.017176885795990415 1106 12-13 -0.02110741416926487 1106 14-15 -0.15453144904819993 1106 16-17 -0.009455423881092884 1106 18-19 -0.12061097576269475 1106 20-21 -0.02023042571756406 1106 22-23 -0.04576093302963358 1106 24-25 0.04271530738301266 1106 26-27 0.07326564939239688 1106 28-29 0.12479044687276541 1106 30-31 0.20625850858618122 1106 32-33 0.23651013011353683 1106 34-35 0.3242817742027526 1106 36-37 0.19420431983194675 1106 38-39 0.34125079574337747 1106 40-41 0.22633917634573208 1106 42-43 -0.16399972499616666 1106 44-45 -0.08487360469485594 1106 46-47 0.14871904315160123 1106 48-49 0.24942296511982676 1106 50-51 -0.027116402315463972 1106 52-53 0.07623310020096596 1106 54-55 -0.3199074456435227 1106 56-57 -0.01962543326227717 1106 58-59 0.19982089385749546 1106 60-61 0.16859230412019954 1106 62-63 0.30697661668972387 1106 64-65 0.15045876756442667 1106 66-67 -0.2784945737775324 1106 68-69 -0.1766098632131694 1106 70-71 -0.14756926454190733 1106 72-73 -0.17405656885561172 1106 74-75 -0.2838019903332061 1106 76-77 0.05462368988505162 1106 78-79 0.11443072287007539 1106 80-81 -0.09151034345548936 1106 82-83 -0.32253762873053304 1106 84-85 -0.1341074625474974 1106 86-87 0.29219763310147684 1106 88-89 0.5442345501183468 1106 90-91 0.09134989979523311 1106 92-93 0.4597851963505093 1106 94-95 0.10245764716903238 1106 96-97 0.19979656266999157 1106 98-99 0.2371925154570711 1106 100-101 0.24102544413395321 1107 1 0.0444333307065925 1107 2 0.031978519269241445 1107 3 -0.11846448351487737 1107 4 -0.08200642047060569 1107 5 0.07830011956173877 1107 6 4.879415821861244E-4 1107 7 -0.07884208433607398 1107 8 -0.08354546960219977 1107 9 -0.025792874275921918 1107 10-11 -0.04732602515308315 1107 12-13 -0.04912660629610599 1107 14-15 -4.95850192926639E-4 1107 16-17 -0.022249098831125025 1107 18-19 0.12377383111599727 1107 20-21 0.16412544013989105 1107 22-23 0.22894360271398284 1107 24-25 0.029548434251019273 1107 26-27 -0.10933023859325885 1107 28-29 -0.06132593499373229 1107 30-31 0.07527078995447312 1107 32-33 -0.060149329101584215 1107 34-35 0.19965080182102923 1107 36-37 0.26374591299803285 1107 38-39 0.11520758977995627 1107 40-41 0.04362382189086844 1107 42-43 0.05082305026373035 1107 44-45 -0.07601882533650439 1107 46-47 -0.06671586322866574 1107 48-49 0.026652171765597643 1107 50-51 -0.004915819189939441 1107 52-53 0.17255751516505313 1107 54-55 0.1386393039536813 1107 56-57 0.12265428832703407 1107 58-59 0.2452073894108615 1107 60-61 0.27502751078104737 1107 62-63 0.22804560480873093 1107 64-65 0.1067746691972431 1107 66-67 0.018649747098642422 1107 68-69 0.05363807814278587 1107 70-71 0.19531053389340158 1107 72-73 0.06842592284900206 1107 74-75 -0.08987185561592526 1107 76-77 -0.06234609155588444 1107 78-79 0.4223162632298312 1107 80-81 0.5672934204916835 1107 82-83 0.23015434904224819 1107 84-85 0.43006163739642744 1107 86-87 0.5113674810561797 1107 88-89 0.7669940425903441 1107 90-91 0.597657137418345 1107 92-93 0.7926427266664717 1107 94-95 0.4594280313236858 1107 96-97 0.8292244904864106 1107 98-99 0.607299637057789 1107 100-101 0.4888352255318402 1108 1 -0.011204919937870983 1108 2 0.02776933036150453 1108 3 0.062258305315261 1108 4 -0.029038750430935067 1108 5 -0.0653079408911239 1108 6 -0.014833655266201617 1108 7 0.004689370797265724 1108 8 0.07875985274609576 1108 9 -0.06293638161002235 1108 10-11 -0.06921935008722357 1108 12-13 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1843.0 11 2839.0 12 4261.0 13 5980.0 14 8198.0 15 11038.0 16 14146.0 17 18087.0 18 18309.0 19 15731.0 20 15453.0 21 15748.0 22 16915.0 23 18184.0 24 20863.0 25 23878.0 26 28506.0 27 34468.0 28 40896.0 29 49409.0 30 58071.0 31 69303.0 32 83850.0 33 101337.0 34 123717.0 35 146906.0 36 151207.0 37 114996.0 38 35921.0 39 1168.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.669226172382075 19.86377493583284 15.309322808084143 22.15767608370094 2 17.626884941375113 21.062269893123485 37.55671381447181 23.754131351029585 3 21.094122143434475 23.732922044814597 29.931053852359017 25.24190195939191 4 15.026963371462628 17.503971302898513 34.85443609094543 32.61462923469343 5 14.249617231026507 37.12815161082109 33.82624562478508 14.795985533367324 6 33.70920089646528 35.78425154005171 15.882498900960373 14.624048662522629 7 29.338972543556675 32.86563313059942 19.408361637094323 18.38703268874958 8 27.37756530036813 34.95382818371707 18.472157285764208 19.196449230150588 9 26.597088304308663 16.45362581670535 19.36220986963831 37.58707600934768 10-11 25.887988115212863 26.389596981570442 26.0678237100546 21.654591193162098 12-13 26.86958408829976 23.492001943564812 26.860807728303044 22.777606239832387 14-15 23.100735857893177 24.9697813968295 25.306833513248712 26.622649232028607 16-17 22.490384880056837 28.15465892005079 26.481522334484502 22.87343386540787 18-19 22.666146467874135 26.133446947302748 29.094311562375587 22.106095022447537 20-21 22.891499457062455 25.28409476234814 29.761394707216642 22.063011073372763 22-23 22.84785701235153 25.167967562573452 29.813693834287964 22.170481590787055 24-25 22.833338586063455 25.560861921150803 29.645544441487843 21.960255051297896 26-27 22.2501206953649 25.78775171467217 29.478197030694524 22.483930559268405 28-29 22.38438541943421 26.282605174701423 29.322616599926437 22.010392805937926 30-31 22.398148347610874 25.840436201048856 29.27295038085413 22.48846507048614 32-33 22.214740120866967 25.682494635426412 29.397304768017847 22.705460475688778 34-35 23.028142461861947 25.8840427632957 29.017637166323208 22.070177608519145 36-37 23.100540161011065 25.457022492081094 29.06588847310764 22.376548873800196 38-39 22.48899602931413 25.863719932587713 29.14033374189265 22.506950296205506 40-41 22.891336014168342 25.64357395549771 29.168024838485607 22.297065191848336 42-43 22.707736818357173 25.73290305129753 28.89423128709996 22.665128843245338 44-45 22.789666012414827 25.907973893755887 29.061507651555047 22.240852442274242 46-47 23.24766010274724 26.320622770505363 28.323946617391393 22.107770509355998 48-49 23.056774272818735 26.46734755263223 28.021042519868484 22.45483565468055 50-51 23.0141291417438 26.65707649874299 28.095322439477027 22.23347192003619 52-53 23.469662118118638 26.319027068687785 28.01968617332354 22.191624639870046 54-55 22.98393048484602 26.539313704605277 28.118460115831994 22.358295694716713 56-57 22.99370415847872 26.73522599525917 27.865581270290345 22.405488575971763 58-59 22.90757615287462 26.4139314342886 27.89653788555148 22.781954527285304 60-61 22.676478637142992 26.596798862583743 28.155600075636265 22.571122424636997 62-63 22.853322291076754 26.604936941853424 28.253456489599614 22.288284277470204 64-65 22.487986359940862 26.658512630378812 28.148898128002415 22.704602881677914 66-67 22.48726829412295 26.740013100711923 28.03660061258993 22.7361179925752 68-69 22.999190576701135 26.280366837500598 28.18604209559652 22.53440049020175 70-71 22.78243323783058 26.559738687870354 27.714587985801447 22.943240088497625 72-73 22.725347005306507 26.75928120015925 27.658379389277044 22.8569924052572 74-75 22.743617791117845 27.087596849127195 27.421696917183873 22.74708844257109 76-77 22.83361537362959 26.70482787563419 27.77358906050662 22.687967690229595 78-79 22.810996300365336 26.906005982286114 27.727393492887558 22.555604224460993 80-81 23.035272190826788 26.85921202648546 27.43992781044977 22.66558797223798 82-83 23.039669561935796 27.019032342096267 27.321697205087258 22.61960089088068 84-85 22.926529578262663 27.226323526771733 27.310299981449383 22.53684691351622 86-87 23.25289251450795 27.04329027988267 27.00583110499779 22.697986100611594 88-89 22.836208389083165 27.32715158449201 26.82905326213312 23.007586764291705 90-91 22.8717002342892 27.23818391363094 27.043428273715847 22.846687578364016 92-93 23.07516166479836 26.812221499722742 27.192836883110665 22.919779952368224 94-95 22.92648521941299 26.57246440986559 27.36967703793063 23.131373332790794 96-97 23.216703487486107 26.20174298509535 27.270304707240577 23.31124882017797 98-99 22.85112820107758 26.62328751275564 26.89028831938291 23.635295966783872 100-101 23.288350499095635 26.144896107843913 27.09685191966918 23.46990147339127 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 594.0 1 428.0 2 366.0 3 501.0 4 409.5 5 355.5 6 767.5 7 1699.0 8 2596.0 9 2882.5 10 2260.0 11 1491.5 12 1367.5 13 1431.0 14 1503.5 15 1603.5 16 1695.0 17 1811.5 18 2004.0 19 2240.5 20 2528.5 21 2917.5 22 3531.0 23 4373.5 24 5311.0 25 6438.5 26 7915.0 27 9456.0 28 10633.5 29 11947.5 30 13431.5 31 15992.5 32 20146.5 33 21578.5 34 20881.0 35 21715.0 36 23690.5 37 26251.0 38 28295.5 39 29634.0 40 31446.0 41 34038.5 42 35735.5 43 36662.5 44 37728.0 45 40410.5 46 42739.5 47 44086.0 48 44427.0 49 44224.5 50 44787.5 51 44460.0 52 44887.0 53 46225.0 54 47481.0 55 45614.5 56 42021.5 57 39049.0 58 36419.0 59 32457.0 60 26385.0 61 22053.5 62 18799.5 63 14484.5 64 10455.5 65 7783.0 66 5842.0 67 4525.5 68 3589.0 69 2815.5 70 2185.5 71 1550.0 72 1075.0 73 784.5 74 574.0 75 395.5 76 274.5 77 198.0 78 128.0 79 75.5 80 46.5 81 26.5 82 16.0 83 10.0 84 7.0 85 2.5 86 2.0 87 2.0 88 1.5 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011568838177485125 3 0.005584956361544544 4 0.0 5 0.0 6 0.0 7 0.0 8 3.989254543960388E-4 9 6.382807270336622E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.994627271980194E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.004029147089399993 26-27 0.016914439266392044 28-29 0.0 30-31 0.0 32-33 4.3881799983564276E-4 34-35 0.015119274721609873 36-37 0.0029520483625306874 38-39 0.014600671630895021 40-41 0.012007656177320768 42-43 0.006422699815776225 44-45 0.0021541974537386097 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.3881799983564276E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.989254543960389E-5 84-85 0.0031515110897287067 86-87 1.994627271980194E-4 88-89 0.0 90-91 1.1967763631881165E-4 92-93 1.5957018175841555E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1253367.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.329001817586835 #Duplication Level Percentage of deduplicated Percentage of total 1 81.72370088378544 50.120330000416004 2 10.911292667540206 13.383573756795904 3 3.0679588126135373 5.644645547851715 4 1.3734912807842345 3.36939397022624 5 0.7592510768144954 2.3282055334980476 6 0.4427513491187635 1.6292098976906204 7 0.32068211977430006 1.3766980014553958 8 0.2208499163000692 1.0835603934544675 9 0.16441108987228906 0.9074851226648143 >10 0.9257001103456187 10.211093133730898 >50 0.05481468064021948 2.3120417041162344 >100 0.02976663285297509 3.606308560062853 >500 0.003509591416156967 1.4975902683200797 >1k 0.0015598184071808742 1.5724458662096015 >5k 2.5996973453014567E-4 0.957418243507088 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6383 0.5092682350819832 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5627 0.4489507063773021 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3625 0.28922095443712814 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1768 0.14106004067443934 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1753 0.1398632643112512 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1731 0.13810799231190865 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1694 0.13515594394937794 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1575 0.12566151813475224 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1526 0.12175204868167105 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1523 0.12151269340903344 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.978509087920776E-5 0.0 0.0 0.0 0.0 3 7.978509087920776E-5 0.0 0.0 7.978509087920776E-5 0.0 4 7.978509087920776E-5 0.0 0.0 7.978509087920776E-5 0.0 5 7.978509087920776E-5 0.0 0.0 7.978509087920776E-5 0.0 6 7.978509087920776E-5 0.0 0.0 7.978509087920776E-5 0.0 7 2.393552726376233E-4 0.0 0.0 7.978509087920776E-5 7.978509087920776E-5 8 2.393552726376233E-4 0.0 0.0 7.978509087920776E-5 7.978509087920776E-5 9 2.393552726376233E-4 0.0 0.0 7.978509087920776E-5 7.978509087920776E-5 10-11 2.393552726376233E-4 0.0 0.0 7.978509087920776E-5 7.978509087920776E-5 12-13 2.7924781807722715E-4 0.0 0.0 7.978509087920776E-5 7.978509087920776E-5 14-15 3.1914036351683104E-4 0.0 0.0 7.978509087920776E-5 7.978509087920776E-5 16-17 3.989254543960388E-4 0.0 0.0 7.978509087920776E-5 7.978509087920776E-5 18-19 5.186030907148504E-4 0.0 0.0 7.978509087920776E-5 7.978509087920776E-5 20-21 5.584956361544543E-4 0.0 0.0 1.1967763631881163E-4 7.978509087920776E-5 22-23 5.584956361544543E-4 0.0 0.0 1.5957018175841552E-4 7.978509087920776E-5 24-25 7.180658179128699E-4 0.0 0.0 3.1914036351683104E-4 7.978509087920776E-5 26-27 7.978509087920776E-4 0.0 0.0 6.382807270336621E-4 7.978509087920776E-5 28-29 9.574210905504933E-4 0.0 0.0 0.0015159167267049475 7.978509087920776E-5 30-31 0.0011169912723089086 0.0 0.0 0.004388179998356427 7.978509087920776E-5 32-33 0.0011169912723089086 0.0 0.0 0.01212733381363958 7.978509087920776E-5 34-35 0.0011568838177485126 0.0 0.0 0.026249294899259354 7.978509087920776E-5 36-37 0.0014361316358257397 0.0 0.0 0.04962632652686723 7.978509087920776E-5 38-39 0.0015159167267049475 0.0 0.0 0.08768381487624934 7.978509087920776E-5 40-41 0.0015159167267049475 0.0 0.0 0.1262200137709067 7.978509087920776E-5 42-43 0.0015159167267049475 0.0 0.0 0.1674689057554571 7.978509087920776E-5 44-45 0.0017153794539029668 0.0 0.0 0.21609791864633424 1.1967763631881163E-4 46-47 0.001994627271980194 0.0 0.0 0.2741814648063975 2.393552726376233E-4 48-49 0.002074412362859402 0.0 0.0 0.336972331328334 2.393552726376233E-4 50-51 0.002074412362859402 7.978509087920776E-5 0.0 0.4076619218473121 2.393552726376233E-4 52-53 0.002114304908299006 7.978509087920776E-5 0.0 0.4720484901868327 2.393552726376233E-4 54-55 0.0021541974537386097 7.978509087920776E-5 0.0 0.5448523856141099 2.393552726376233E-4 56-57 0.0023137676354970252 7.978509087920776E-5 0.0 0.6312596390362919 2.393552726376233E-4 58-59 0.0023137676354970252 7.978509087920776E-5 0.0 0.724289055001448 2.393552726376233E-4 60-61 0.0023137676354970252 7.978509087920776E-5 0.0 0.8246188067820519 2.393552726376233E-4 62-63 0.0023137676354970252 7.978509087920776E-5 0.0 0.9232331791087527 2.393552726376233E-4 64-65 0.0023137676354970252 7.978509087920776E-5 0.0 1.0226852948896852 2.393552726376233E-4 66-67 0.0023935527263762328 7.978509087920776E-5 0.0 1.1331078606665086 2.393552726376233E-4 68-69 0.0023935527263762328 7.978509087920776E-5 0.0 1.2641548724356075 2.393552726376233E-4 70-71 0.0023935527263762328 7.978509087920776E-5 0.0 1.3969571562040488 2.393552726376233E-4 72-73 0.0023935527263762328 7.978509087920776E-5 0.0 1.5402910719685456 2.393552726376233E-4 74-75 0.0024334452718158366 7.978509087920776E-5 0.0 1.6833856324604048 2.393552726376233E-4 76-77 0.0024733378172554408 7.978509087920776E-5 0.0 1.8385676342204638 2.393552726376233E-4 78-79 0.0024733378172554408 7.978509087920776E-5 0.0 2.0222728059698394 2.393552726376233E-4 80-81 0.002792478180772272 1.5957018175841552E-4 0.0 2.2082917453547126 2.393552726376233E-4 82-83 0.0028323707262118756 1.5957018175841552E-4 0.0 2.3969037001931595 2.393552726376233E-4 84-85 0.0029121558170910836 1.5957018175841552E-4 0.0 2.586313505940399 2.393552726376233E-4 86-87 0.0029520483625306874 1.5957018175841552E-4 0.0 2.781308268049183 2.393552726376233E-4 88-89 0.002991940907970291 1.5957018175841552E-4 0.0 2.9926589737882043 2.393552726376233E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2690 0.0 61.108128 1 GTATCAA 4620 0.0 42.058765 1 TGGTATC 795 0.0 40.038963 2 GTGGTAT 990 0.0 34.072056 1 ATCAACG 6145 0.0 31.386625 3 TCAACGC 6145 0.0 31.381618 4 TATCAAC 6185 0.0 31.339754 2 CAACGCA 6350 0.0 30.29371 5 AACGCAG 6585 0.0 29.212616 6 TAAGGTG 770 0.0 26.524605 5 GTAAGGT 765 0.0 26.077084 4 ACGCAGA 7435 0.0 25.872906 7 CGCAGAG 7555 0.0 25.524822 8 AGGTAAG 815 0.0 23.900192 2 GGTAAGG 910 0.0 23.491545 3 CCTATAC 285 3.6379788E-12 23.335852 3 GCAGAGT 8275 0.0 22.729942 9 GAGTACT 5775 0.0 22.413155 12-13 CAGAGTA 7920 0.0 22.010035 10-11 GTACTTT 5985 0.0 21.547365 14-15 >>END_MODULE