FastQCFastQC Report
Thu 26 May 2016
SRR938208_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938208_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214881
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59330.48836058840330865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49040.403660934692369No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30370.2499833317008003No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA20700.1703870584855636No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA19740.1624850499760882No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA18710.1540068533461302No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA17850.14692797072305847No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA15460.12725526203801032No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA14000.11523762409651646No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC13630.11219205831682279No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG12920.10634786452335661No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12770.10511317569375107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22700.056.431931
GTATCAA41150.046.2901571
ATCAACG51050.037.083753
TCAACGC51650.036.7450564
TATCAAC52300.036.5597152
CAACGCA52950.035.9327435
AACGCAG54100.035.252486
TGGTATC8750.033.1589052
GTGGTAT9700.032.4019131
ACGCAGA62850.030.3346027
CGCAGAG63350.030.015188
GCAGAGT70800.026.386819
GAGTACT46450.022.92066212-13
CAGAGTA67800.022.7488210-11
GTGTAAG5550.021.4509011
AGAGTAC64500.021.44406510-11
GTACTTT49300.021.20781314-15
GTATAGC4450.020.3325611
AGTACTT49300.020.29411312-13
GTACATG39500.020.2539941
Produced by FastQC (version 0.11.5)