##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938208_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1214881 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5187100629609 33.0 31.0 34.0 31.0 34.0 2 32.668920659718935 34.0 31.0 34.0 31.0 34.0 3 32.69765598441329 34.0 31.0 34.0 31.0 34.0 4 36.19170272643987 37.0 37.0 37.0 35.0 37.0 5 36.19106480387791 37.0 37.0 37.0 35.0 37.0 6 36.243870798868365 37.0 37.0 37.0 35.0 37.0 7 36.20387017329269 37.0 37.0 37.0 35.0 37.0 8 36.20105096713176 37.0 37.0 37.0 35.0 37.0 9 37.94151443639336 39.0 38.0 39.0 37.0 39.0 10-11 37.86693264607809 39.0 38.0 39.0 35.0 39.0 12-13 37.74911616857948 39.0 38.0 39.0 35.0 39.0 14-15 39.06248965948105 40.0 39.0 41.0 36.0 41.0 16-17 38.88423886783973 40.0 38.0 41.0 36.0 41.0 18-19 39.06075574480134 40.0 39.0 41.0 36.0 41.0 20-21 39.055096342769374 40.0 39.0 41.0 36.0 41.0 22-23 38.961839060780434 40.0 39.0 41.0 36.0 41.0 24-25 38.84901278396814 40.0 38.5 41.0 35.5 41.0 26-27 38.67729514248721 40.0 38.0 41.0 35.0 41.0 28-29 38.5145631547452 40.0 38.0 41.0 35.0 41.0 30-31 38.299051512041096 40.0 38.0 41.0 34.5 41.0 32-33 38.016290484417816 40.0 38.0 41.0 34.0 41.0 34-35 37.79041239430035 40.0 37.5 41.0 33.5 41.0 36-37 37.54424095857948 40.0 37.0 41.0 33.0 41.0 38-39 37.24751724654514 39.5 36.5 41.0 32.5 41.0 40-41 37.14538707906371 39.0 37.0 41.0 32.0 41.0 42-43 36.9353035400175 39.0 36.0 41.0 31.5 41.0 44-45 36.625626295908816 39.0 36.0 41.0 31.0 41.0 46-47 36.24739912798044 39.0 35.5 40.0 30.0 41.0 48-49 35.833575057968645 39.0 35.0 40.0 29.0 41.0 50-51 35.108573596920195 38.5 34.5 39.5 27.5 40.5 52-53 35.19629700357484 38.0 35.0 40.0 28.0 40.5 54-55 35.280428288861216 38.0 35.0 40.0 28.0 41.0 56-57 34.958360530784496 38.0 34.5 40.0 27.0 41.0 58-59 34.53805640223199 38.0 34.0 40.0 26.0 41.0 60-61 34.03456346753303 37.0 34.0 40.0 24.5 41.0 62-63 33.49016158784276 36.5 33.0 39.0 23.0 41.0 64-65 32.93245099725817 36.0 32.5 39.0 21.5 40.0 66-67 32.35195381276026 35.0 31.5 38.5 19.0 40.0 68-69 31.65865463366371 35.0 31.0 38.0 14.0 40.0 70-71 31.057382986481805 35.0 30.5 37.0 9.0 39.5 72-73 30.35031620380926 34.0 29.5 36.5 4.5 39.0 74-75 29.677580355606842 34.0 29.0 36.0 2.0 39.0 76-77 28.94157164364246 34.0 28.5 35.5 2.0 37.5 78-79 28.173945431692488 33.5 26.5 35.0 2.0 37.0 80-81 27.456623323601242 33.0 26.0 35.0 2.0 36.5 82-83 26.719056845896844 32.0 24.5 35.0 2.0 36.0 84-85 25.98402353810785 32.0 23.0 34.5 2.0 35.5 86-87 25.23262813394892 31.0 19.5 34.0 2.0 35.0 88-89 24.30997768505722 31.0 15.0 34.0 2.0 35.0 90-91 23.35100762955384 30.0 4.5 34.0 2.0 35.0 92-93 22.362345365513164 29.5 2.0 34.0 2.0 35.0 94-95 21.213959227282345 29.0 2.0 34.0 2.0 35.0 96-97 19.850122769225955 27.5 2.0 33.5 2.0 35.0 98-99 18.17239589721133 24.5 2.0 33.0 2.0 34.5 100-101 14.053484250720853 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12323023777321396 1101 2 0.09250301580477327 1101 3 0.03722578406262045 1101 4 0.019892026700723875 1101 5 0.03511271537801264 1101 6 0.03663422909468039 1101 7 0.1116673043580576 1101 8 0.17911481077144487 1101 9 0.08172745384926117 1101 10-11 0.14541948215287448 1101 12-13 0.16151489603655733 1101 14-15 0.2581779567098934 1101 16-17 0.28390463361932206 1101 18-19 0.25349069600378016 1101 20-21 0.19604016197907015 1101 22-23 0.26562391680212727 1101 24-25 0.21445206996185817 1101 26-27 0.1733622170212641 1101 28-29 0.10783388889289114 1101 30-31 0.01590864795578284 1101 32-33 0.0954865208283664 1101 34-35 0.1449254669992044 1101 36-37 0.24881244741622055 1101 38-39 0.14432661995228102 1101 40-41 0.12235025022440027 1101 42-43 0.26758923674775303 1101 44-45 0.4704079878784668 1101 46-47 0.12364913178738846 1101 48-49 0.20107360384872663 1101 50-51 0.07973266326781925 1101 52-53 -0.012059775245418791 1101 54-55 0.012428822957488705 1101 56-57 -0.025850251530194157 1101 58-59 0.042285611514714105 1101 60-61 0.10106212191332276 1101 62-63 0.03644336991536079 1101 64-65 -0.11902793775403353 1101 66-67 0.02147165715187782 1101 68-69 0.05897905029332762 1101 70-71 -0.009416556672832144 1101 72-73 -0.16092496304757375 1101 74-75 -0.5105730498166849 1101 76-77 -0.8321703038235917 1101 78-79 -0.9110505574410013 1101 80-81 -1.0237261360258998 1101 82-83 -0.9381393012568289 1101 84-85 -1.2748596157604624 1101 86-87 -1.316545139249989 1101 88-89 -1.4570796693956964 1101 90-91 -1.5811014770577678 1101 92-93 -1.5574393993056361 1101 94-95 -1.7055889365276151 1101 96-97 -1.4894812841223413 1101 98-99 -1.7762196240125974 1101 100-101 -1.715577456585093 1102 1 0.14835284749887023 1102 2 0.1666483305274724 1102 3 0.1254524464673068 1102 4 0.12218757486635923 1102 5 0.1105665318101785 1102 6 0.08089211858111867 1102 7 0.1162277443635702 1102 8 0.1642882402111141 1102 9 0.11072572101913636 1102 10-11 0.17477914503299274 1102 12-13 0.11076372259091727 1102 14-15 0.14279365287330137 1102 16-17 0.10380152368461637 1102 18-19 0.08558188735720051 1102 20-21 0.0579331028615897 1102 22-23 0.2102630787628641 1102 24-25 0.11568791867164663 1102 26-27 0.04932092818447131 1102 28-29 0.09529252310372982 1102 30-31 -0.011026548185512297 1102 32-33 -0.09975408459668245 1102 34-35 0.15013299225484644 1102 36-37 0.21256134219959932 1102 38-39 0.12400901543259124 1102 40-41 0.186755950556325 1102 42-43 0.3697265185161456 1102 44-45 0.20827428268140835 1102 46-47 0.06694302937158625 1102 48-49 0.11291703974990241 1102 50-51 0.1924298653796157 1102 52-53 0.008373725139961152 1102 54-55 0.06711765190453178 1102 56-57 0.21894113279606842 1102 58-59 0.16648111662441778 1102 60-61 0.1512269619739115 1102 62-63 -0.022293402534323548 1102 64-65 -0.09707065597317666 1102 66-67 -0.38873895554470295 1102 68-69 -0.28769539261206845 1102 70-71 -0.5398778533731026 1102 72-73 -0.5305523952251257 1102 74-75 -0.47555162465893375 1102 76-77 -0.6552156982402799 1102 78-79 -0.8403173166997462 1102 80-81 -0.7619074058831892 1102 82-83 -0.697514871779152 1102 84-85 -0.5802305791105766 1102 86-87 -0.8605597097280722 1102 88-89 -0.9704282561203854 1102 90-91 -1.1458741809643946 1102 92-93 -1.3044328050801113 1102 94-95 -1.1631975312653147 1102 96-97 -1.4883220139173616 1102 98-99 -1.5099096175261657 1102 100-101 -1.6108776923023296 1103 1 0.13374653877491482 1103 2 0.1277656445859634 1103 3 0.19910428482502596 1103 4 -0.009432439106596746 1103 5 -0.008608410894176188 1103 6 0.030093244252832108 1103 7 0.07633299858647291 1103 8 0.10743659512417025 1103 9 0.026339518985878385 1103 10-11 0.10013157038541465 1103 12-13 0.12733538622140372 1103 14-15 0.10017613966754624 1103 16-17 0.150059187589342 1103 18-19 0.07266343326227798 1103 20-21 0.04571329652413425 1103 22-23 0.05841492691471473 1103 24-25 0.13313452435354378 1103 26-27 0.1200247341823939 1103 28-29 0.08022262568089644 1103 30-31 0.09686229499745025 1103 32-33 0.1002224094974622 1103 34-35 -0.14612630400444715 1103 36-37 -0.09569902488429705 1103 38-39 -0.1713002504649097 1103 40-41 -0.08095019950277305 1103 42-43 -0.02507802334721987 1103 44-45 -0.08791318997665343 1103 46-47 0.04108979763599763 1103 48-49 0.06136075289949616 1103 50-51 0.056500435332544896 1103 52-53 0.0028269842991051064 1103 54-55 -0.2022992057476145 1103 56-57 0.05082819997725352 1103 58-59 0.04400555297237929 1103 60-61 0.05424877676042428 1103 62-63 0.02363126103740143 1103 64-65 0.047474341513002116 1103 66-67 -0.2174004900885862 1103 68-69 -0.09148963758278583 1103 70-71 -0.35029945791235306 1103 72-73 -0.42502067204634386 1103 74-75 -0.2555787870390418 1103 76-77 -0.5423514383171941 1103 78-79 -0.6744178566826378 1103 80-81 -0.8879055907049072 1103 82-83 -1.0161113080226478 1103 84-85 -1.0876512630018453 1103 86-87 -1.2716845326176056 1103 88-89 -0.9774604396231581 1103 90-91 -0.9422640655895727 1103 92-93 -1.1572597167305574 1103 94-95 -1.583519039822118 1103 96-97 -1.7736771816254695 1103 98-99 -2.2028441339900944 1103 100-101 -2.0628766737130757 1104 1 0.20768228116792642 1104 2 0.10482663021520722 1104 3 0.08818279787690386 1104 4 0.1396475153451675 1104 5 0.0763298251206379 1104 6 0.15695257803780294 1104 7 0.16094663134660436 1104 8 0.033862355822961376 1104 9 0.06861462456841849 1104 10-11 0.09563267305632195 1104 12-13 0.11821853193251997 1104 14-15 0.08502760340913795 1104 16-17 0.09158707147016543 1104 18-19 0.05753421176235918 1104 20-21 0.0869266669494877 1104 22-23 0.15533234911514882 1104 24-25 0.08525323804828844 1104 26-27 0.03549412605578084 1104 28-29 -0.011863800656364276 1104 30-31 -0.008089778456280783 1104 32-33 0.01593136203216261 1104 34-35 -0.005116747523196352 1104 36-37 -0.060114032739924994 1104 38-39 -0.16972249414796892 1104 40-41 -0.12916992650163195 1104 42-43 -0.16321919405220342 1104 44-45 -0.341810014572701 1104 46-47 -0.29964329834682246 1104 48-49 -0.16833554414419183 1104 50-51 -0.2955084436907711 1104 52-53 -0.34541378713811355 1104 54-55 -0.2500807471806539 1104 56-57 -0.263744064606108 1104 58-59 -0.22549759648637746 1104 60-61 -0.11573320574097323 1104 62-63 -0.060016057238108544 1104 64-65 -0.29868447192661307 1104 66-67 -0.545592542209608 1104 68-69 -0.408290018112357 1104 70-71 -0.39318802328704905 1104 72-73 -0.43925363109617166 1104 74-75 -0.6738058651773287 1104 76-77 -0.5706841710672421 1104 78-79 -0.7598311781003986 1104 80-81 -0.8542367955710723 1104 82-83 -0.8583596035672798 1104 84-85 -1.0124816823050722 1104 86-87 -0.9476667745709904 1104 88-89 -0.7944006359418196 1104 90-91 -1.1365783195930597 1104 92-93 -1.2920937395335024 1104 94-95 -1.2490511325906155 1104 96-97 -1.5830779916922069 1104 98-99 -1.8554310143683175 1104 100-101 -1.5555889558815164 1105 1 0.10166097727758938 1105 2 0.1277656445859634 1105 3 0.14257258123450356 1105 4 0.08453241956414104 1105 5 0.1136222995718299 1105 6 0.10495955441325577 1105 7 0.1435598893427752 1105 8 0.1334106210982 1105 9 0.09051064198052927 1105 10-11 0.04703760552674652 1105 12-13 0.09754165054531683 1105 14-15 0.06312495555754793 1105 16-17 0.043489286901795765 1105 18-19 0.10245716893836487 1105 20-21 0.13051085190992495 1105 22-23 0.09508414005451016 1105 24-25 0.058268214193120116 1105 26-27 0.12117064709300962 1105 28-29 0.07831277082986077 1105 30-31 0.0857851368614746 1105 32-33 0.015424854111664388 1105 34-35 -0.11098497474547031 1105 36-37 0.03684490177727895 1105 38-39 0.05711838971843264 1105 40-41 0.04051656902282019 1105 42-43 -0.15189238545569594 1105 44-45 -0.1253463450568617 1105 46-47 -0.117810125969811 1105 48-49 -0.2426881393846969 1105 50-51 -0.025623323261804387 1105 52-53 -0.2798315336535353 1105 54-55 -0.33407919046877765 1105 56-57 -0.417468209495631 1105 58-59 -0.43918772433854514 1105 60-61 -0.4774548137666912 1105 62-63 -0.6199898237601573 1105 64-65 -0.6324339854541492 1105 66-67 -0.5604104213338132 1105 68-69 -0.6296867346034141 1105 70-71 -0.6734468987068531 1105 72-73 -0.7477861418706375 1105 74-75 -0.8273893294378141 1105 76-77 -0.8322674047037495 1105 78-79 -0.8230045640928729 1105 80-81 -0.9432913813848138 1105 82-83 -1.2663022935077493 1105 84-85 -1.2725252125816766 1105 86-87 -1.1467800253601546 1105 88-89 -1.2303252218997045 1105 90-91 -1.2520425224268514 1105 92-93 -1.4070687312454524 1105 94-95 -1.4039926838251766 1105 96-97 -1.6915534230311238 1105 98-99 -2.035922820009958 1105 100-101 -1.950577208472433 1106 1 0.23883367026315483 1106 2 0.14402965436548953 1106 3 0.22268155157043168 1106 4 0.16346038779271055 1106 5 0.3041559694941043 1106 6 0.17145859497436078 1106 7 0.1921046233900512 1106 8 0.06329037543372351 1106 9 0.26122526521946554 1106 10-11 0.23418299577542 1106 12-13 0.24841216338896288 1106 14-15 0.29002306388223786 1106 16-17 -0.05576200989465718 1106 18-19 0.2870483998473361 1106 20-21 0.3958947912169748 1106 22-23 0.3713530823725506 1106 24-25 0.3667570595045717 1106 26-27 0.16948379244182377 1106 28-29 0.11786531339306094 1106 30-31 0.22695900082366194 1106 32-33 0.29602380330718603 1106 34-35 0.36765038300058706 1106 36-37 0.3954471353216107 1106 38-39 0.14431504361263592 1106 40-41 0.29049167240227547 1106 42-43 0.01162093206410475 1106 44-45 0.155667659208639 1106 46-47 0.2138940660863966 1106 48-49 0.37193055466892844 1106 50-51 0.2989738026322115 1106 52-53 0.3518294819815466 1106 54-55 0.36159788732965836 1106 56-57 0.019888217207721937 1106 58-59 0.1533241074044156 1106 60-61 0.28902223756445977 1106 62-63 0.03140836376586975 1106 64-65 0.054836054988648186 1106 66-67 -0.03308061563439679 1106 68-69 -0.06408129891923409 1106 70-71 -0.32190160390079114 1106 72-73 -0.23524927315034816 1106 74-75 -0.3305981529338098 1106 76-77 -0.7478531570194704 1106 78-79 -0.7255628672996259 1106 80-81 -0.8750803222057151 1106 82-83 -0.7590294726638334 1106 84-85 -0.8521716738863176 1106 86-87 -0.7636303923263164 1106 88-89 -1.0810805085049182 1106 90-91 -1.3187721491379527 1106 92-93 -1.3536867007907034 1106 94-95 -1.7305671695010965 1106 96-97 -1.68106409248621 1106 98-99 -1.8844668188550209 1106 100-101 -1.9584876501485073 1107 1 0.09572703496451851 1107 2 0.036055346275951194 1107 3 0.07972962254326177 1107 4 0.11140372795446041 1107 5 -0.0074351311609959225 1107 6 0.05316231112569625 1107 7 0.027759217632848276 1107 8 0.06258819567570129 1107 9 0.08974670004011642 1107 10-11 0.1117129767838918 1107 12-13 0.14101967091088596 1107 14-15 0.022898346621289534 1107 16-17 0.031160242201870858 1107 18-19 0.08768636316594325 1107 20-21 0.14124126658133918 1107 22-23 0.18080663581556422 1107 24-25 0.11537403878459429 1107 26-27 -0.037178446424661615 1107 28-29 -0.07579126269042291 1107 30-31 -0.1287800683780489 1107 32-33 0.06994626078964217 1107 34-35 0.1524166164896954 1107 36-37 -0.05544796048958034 1107 38-39 -0.03606913473702633 1107 40-41 -0.01774390257157421 1107 42-43 -0.079604088207752 1107 44-45 -0.28714336708075194 1107 46-47 -0.2360831280753075 1107 48-49 -0.15891567529927642 1107 50-51 -0.2546769319558351 1107 52-53 -0.2059076320529769 1107 54-55 -0.02086969103707048 1107 56-57 -0.23368136327155042 1107 58-59 -0.242211816458763 1107 60-61 -0.21026524669817803 1107 62-63 -0.18275679584832716 1107 64-65 -0.4926591037719916 1107 66-67 -0.6057895624581136 1107 68-69 -0.5166888331146531 1107 70-71 -0.6649660950771974 1107 72-73 -0.7305930811651997 1107 74-75 -0.7893989392684446 1107 76-77 -1.1862930643024008 1107 78-79 -1.2957268292738888 1107 80-81 -1.0955164205135475 1107 82-83 -1.218685477934514 1107 84-85 -1.4137374347210887 1107 86-87 -1.1287505297481601 1107 88-89 -1.1440399594577002 1107 90-91 -1.36546810330222 1107 92-93 -1.478690034977589 1107 94-95 -1.4494198624571588 1107 96-97 -1.2983707896673558 1107 98-99 -1.117908579945862 1107 100-101 -0.9521216871286402 1108 1 -0.13453506562159845 1108 2 -0.09648388609311098 1108 3 -0.0198177871750147 1108 4 0.09516601761224308 1108 5 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1.2929328601558119 2316 92-93 1.1806901186549084 2316 94-95 1.0159612893525782 2316 96-97 1.065478377071642 2316 98-99 0.9467532332648005 2316 100-101 0.6558509823444965 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2833.0 3 626.0 4 444.0 5 478.0 6 600.0 7 683.0 8 874.0 9 1216.0 10 1590.0 11 2276.0 12 2995.0 13 4053.0 14 5301.0 15 7195.0 16 8926.0 17 12028.0 18 11581.0 19 9621.0 20 9878.0 21 10469.0 22 11695.0 23 13362.0 24 15224.0 25 17883.0 26 20592.0 27 24232.0 28 28874.0 29 34739.0 30 42486.0 31 51972.0 32 66137.0 33 85255.0 34 114081.0 35 152393.0 36 187384.0 37 179395.0 38 72668.0 39 2842.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.87401650078252 17.739371368150735 14.237961689372893 24.148650441693857 2 15.840897655808025 18.999755427331035 39.939210927869176 25.22013598899176 3 19.361055494053943 22.51695370013939 29.380945747335257 28.74104505847141 4 13.028100535640707 15.842933662958385 35.46864441697569 35.66032138442522 5 14.095622746808012 36.40069793291796 34.03717581246846 15.466503507805568 6 33.8342634554366 34.73263936989175 16.491279280705573 14.94181789396608 7 29.602143446001648 32.28656224237428 19.572382522671063 18.53891178895301 8 27.17962656387701 34.173688155304625 19.012296871010516 19.634388409807848 9 26.366056838380537 16.49123905757756 19.517334412296222 37.62536969174569 10-11 25.468142732094122 25.918500592142525 26.36173214561557 22.25162453014778 12-13 26.428306490166666 23.14881060318821 27.035245050361844 23.38763785628328 14-15 22.947649944676705 24.702047477072252 25.263398642982388 27.08690393526865 16-17 22.553492749777064 27.828427599143524 26.35336945395445 23.264710197124966 18-19 22.62148947760081 25.94516996453939 28.850954173152942 22.582386384706858 20-21 22.7307415905165 25.204097274417503 29.47172059812492 22.59344053694107 22-23 22.57156015084868 25.106484209085473 29.4299224441213 22.89203319594455 24-25 22.47348683846992 25.425418820942657 29.45655750751952 22.644536833067907 26-27 21.986603003320813 25.79057858310317 29.10314962122617 23.11966879234985 28-29 22.209389762279024 26.301099503297277 28.9595612699603 22.529949464463403 30-31 22.22244190654135 25.703600818214245 28.923568804716844 23.150388470527563 32-33 21.80210268523543 25.687995527148388 29.1260263847155 23.383875402900678 34-35 22.716915477804704 25.892078958337795 28.789008374618792 22.601997189238705 36-37 22.80031351280555 25.442437327299217 28.73024430687187 23.027004853023367 38-39 22.22932244145064 25.820638031555713 28.805955400375506 23.144084126618136 40-41 22.673623321125834 25.673876145636797 28.846947288148293 22.80555324508907 42-43 22.405169936034643 25.637327043584897 28.62600071978528 23.33150230059518 44-45 22.309988999189763 26.031037765056947 28.881490972089562 22.77748226366373 46-47 22.915993275212703 26.334262116002172 28.077881041427933 22.671863567357196 48-49 22.666323016697763 26.45786362805435 27.86006254868603 23.015750806561847 50-51 22.719739371726757 26.63529870999381 27.90504625517738 22.73991566310205 52-53 23.035756922088936 26.257996525576534 27.935105673519462 22.771140878815075 54-55 22.46059016447443 26.56304235489337 28.082284566085203 22.894082914547003 56-57 22.551163858134657 26.749944940834553 27.841166142832733 22.857725058198053 58-59 22.564415702699165 26.287222925393976 27.79804486226663 23.350316509640223 60-61 22.08989682708605 26.54315778033115 28.148572948285093 23.2183724442977 62-63 22.3322082639687 26.562438879169033 28.352447983213313 22.75290487364896 64-65 22.207107578749397 26.573917468557056 28.093982157767233 23.12499279492631 66-67 22.06815175410463 26.6460208579758 28.105650987297558 23.18017640062201 68-69 22.692592319574743 26.21756084664147 28.22309810618805 22.86674872759574 70-71 22.4280514000625 26.455904835194605 27.72740130266524 23.38864246207765 72-73 22.3707739379234 26.633450116913966 27.79139040535527 23.20438553980736 74-75 22.436217305811095 26.973796890079015 27.56143592581248 23.028549878297415 76-77 22.634014537365506 26.635083146518827 27.58859864834938 23.14230366776629 78-79 22.5591651123531 26.89291105402013 27.64198659592362 22.905937237703153 80-81 22.8653807521184 26.800145892132033 27.30887472048319 23.025598635266377 82-83 22.63828382376369 26.993147298705246 27.2839353202476 23.08463355728347 84-85 22.612893638636624 27.10958036851434 27.32927129462459 22.948254698224442 86-87 22.93689050620145 27.045120550618083 26.96447904102879 23.053509902151674 88-89 22.711953746561463 27.142227676291817 26.801874516134344 23.34394406101237 90-91 22.70907471476984 27.1331804635366 26.917141329308077 23.240603492385485 92-93 22.908830009073462 26.684429185171577 27.0923850032064 23.31435580254856 94-95 22.658479010035386 26.48611651061194 27.278799839223 23.576604640129677 96-97 23.07091079127033 26.06852934963129 27.196615330257735 23.66394452884065 98-99 22.693700879156804 26.339498397621323 26.84357959414093 24.12322112908094 100-101 23.68776710678338 25.15904854359331 26.943523757567167 24.209660592056142 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 612.0 1 437.0 2 371.5 3 478.0 4 387.5 5 370.5 6 703.5 7 1489.0 8 2396.0 9 2692.5 10 2080.0 11 1394.0 12 1251.5 13 1318.5 14 1372.0 15 1400.5 16 1516.0 17 1672.5 18 1797.5 19 1978.5 20 2240.0 21 2718.0 22 3323.0 23 4013.0 24 4908.0 25 6032.0 26 7401.5 27 8889.0 28 10088.5 29 11281.0 30 12824.5 31 15263.0 32 18939.5 33 20133.0 34 19774.0 35 20908.5 36 22807.5 37 25053.0 38 27085.5 39 28530.5 40 30121.5 41 32435.5 42 34040.5 43 35120.0 44 36426.0 45 39183.0 46 41160.0 47 42343.5 48 43042.5 49 42970.0 50 43293.5 51 43012.0 52 43874.0 53 45278.5 54 46672.0 55 45157.0 56 41646.5 57 38659.0 58 36167.5 59 32522.0 60 26304.0 61 21854.5 62 18712.5 63 14557.5 64 10544.0 65 7746.0 66 5849.5 67 4557.5 68 3541.0 69 2778.5 70 2204.5 71 1595.5 72 1106.5 73 818.5 74 618.5 75 433.5 76 302.5 77 199.5 78 127.0 79 83.5 80 51.5 81 38.0 82 28.0 83 21.5 84 15.5 85 10.0 86 6.0 87 5.5 88 5.5 89 3.5 90 2.0 91 1.0 92 2.0 93 2.0 94 1.5 95 1.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12215188154230744 2 0.042637920915711086 3 0.14190690281599597 4 0.11367368491234943 5 0.1775482536972757 6 0.1759020019244683 7 0.1548299792325339 8 0.06181675406891704 9 0.11276824643730538 10-11 0.08795100096223415 12-13 0.13672120973165272 14-15 0.12828416939601492 16-17 0.12667907391752772 18-19 0.11704850104660458 20-21 0.12256344448550928 22-23 0.17635472116199036 24-25 0.19508083507767426 26-27 0.13367564395195908 28-29 0.1134679034407485 30-31 0.08379421523589553 32-33 0.11009308730649339 34-35 0.08025477392435967 36-37 0.07371092312745034 38-39 0.04288485868163219 40-41 0.04938755318422133 42-43 0.0518569308434324 44-45 0.03498285017215678 46-47 0.04671239405340935 48-49 0.03843997889505227 50-51 0.04811170806029561 52-53 0.023952963294347346 54-55 0.04597158075564602 56-57 0.022924055936342738 58-59 0.04461342304307994 60-61 0.030167563736695196 62-63 0.048194020648935985 64-65 0.03778147818592932 66-67 0.03535325682103844 68-69 0.061117097065473906 70-71 0.04317295274187348 72-73 0.04333757791915423 74-75 0.03662910194496416 76-77 0.01794414432360042 78-79 0.020619303454412406 80-81 0.023459087762505135 82-83 0.020619303454412406 84-85 0.018108769500881156 86-87 0.02024889680553075 88-89 0.057701124636898596 90-91 0.09976285743212711 92-93 0.07420479865929255 94-95 0.06395688137356663 96-97 0.05765996834257841 98-99 0.048770208769418566 100-101 0.11647231292612199 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1214881.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.00475970577731 #Duplication Level Percentage of deduplicated Percentage of total 1 81.63768388945846 49.80287288612621 2 10.888730592887308 13.285287866400727 3 3.168709168778135 5.799190242564104 4 1.3732945898662148 3.3511002584012974 5 0.7420729714464805 2.263499165362235 6 0.46840578848216746 1.7144989542689872 7 0.32036474727903214 1.36806420981716 8 0.21388118378555934 1.0438216177940194 9 0.16644887865985306 0.9138756461346379 >10 0.9285836226318358 10.228713397777547 >50 0.05531293953690922 2.3160414220193077 >100 0.030314094055822225 3.5407970461233 >500 0.004311327396297792 1.849628316252852 >1k 0.001751476754745978 2.0349692268976343 >5k 1.34728981134306E-4 0.4876397440600858 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5933 0.48836058840330865 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4904 0.403660934692369 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3037 0.2499833317008003 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2070 0.1703870584855636 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1974 0.1624850499760882 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1871 0.1540068533461302 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1785 0.14692797072305847 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1546 0.12725526203801032 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 1400 0.11523762409651646 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1363 0.11219205831682279 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1292 0.10634786452335661 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1277 0.10511317569375107 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 8.231258864036889E-5 0.0 0.0 0.0 0.0 8 8.231258864036889E-5 0.0 0.0 0.0 0.0 9 8.231258864036889E-5 0.0 0.0 0.0 0.0 10-11 8.231258864036889E-5 0.0 0.0 0.0 0.0 12-13 1.6462517728073778E-4 0.0 0.0 0.0 0.0 14-15 1.6462517728073778E-4 0.0 0.0 0.0 0.0 16-17 2.0578147160092223E-4 0.0 0.0 0.0 0.0 18-19 2.8809406024129115E-4 0.0 0.0 0.0 4.1156294320184446E-5 20-21 3.2925035456147556E-4 0.0 0.0 4.1156294320184446E-5 8.231258864036889E-5 22-23 4.1156294320184446E-4 0.0 0.0 8.231258864036889E-5 8.231258864036889E-5 24-25 4.1156294320184446E-4 0.0 0.0 3.2925035456147556E-4 8.231258864036889E-5 26-27 4.1156294320184446E-4 0.0 0.0 6.173444148027667E-4 8.231258864036889E-5 28-29 4.1156294320184446E-4 0.0 0.0 0.0015639391841670088 8.231258864036889E-5 30-31 4.1156294320184446E-4 0.0 0.0 0.004856442729781765 8.231258864036889E-5 32-33 4.1156294320184446E-4 0.0 0.0 0.010865261700528695 8.231258864036889E-5 34-35 5.761881204825823E-4 0.0 0.0 0.023541400351145506 8.231258864036889E-5 36-37 6.585007091229511E-4 0.0 0.0 0.047617832528453405 8.231258864036889E-5 38-39 7.408132977633201E-4 0.0 0.0 0.08297108934949185 1.2346888296055334E-4 40-41 7.408132977633201E-4 0.0 0.0 0.11873590911373212 1.6462517728073778E-4 42-43 9.877510636844267E-4 0.0 0.0 0.1568466376542229 1.6462517728073778E-4 44-45 0.0013170014182459023 0.0 0.0 0.20648112860436535 1.6462517728073778E-4 46-47 0.0014404703012064557 0.0 0.0 0.27089072921545404 1.6462517728073778E-4 48-49 0.0014816265955266401 0.0 0.0 0.3364527060675079 1.6462517728073778E-4 50-51 0.0014816265955266401 0.0 0.0 0.40477215463901406 1.6462517728073778E-4 52-53 0.001563939184167009 0.0 0.0 0.47325622838780096 1.6462517728073778E-4 54-55 0.0016462517728073778 0.0 0.0 0.5503008113551862 2.0578147160092223E-4 56-57 0.0018931895387284845 0.0 0.0 0.6380460308458196 2.469377659211067E-4 58-59 0.0018931895387284845 0.0 0.0 0.7294953168252694 3.2925035456147556E-4 60-61 0.0018931895387284845 0.0 0.0 0.8284762046653129 3.2925035456147556E-4 62-63 0.0018931895387284845 0.0 0.0 0.9271689984451152 3.2925035456147556E-4 64-65 0.0018931895387284845 0.0 0.0 1.0348750206810378 3.2925035456147556E-4 66-67 0.0020578147160092222 0.0 0.0 1.1595785924711968 3.2925035456147556E-4 68-69 0.0020578147160092222 0.0 0.0 1.295723614082367 3.2925035456147556E-4 70-71 0.0021812835989697757 0.0 0.0 1.4429396788656668 3.2925035456147556E-4 72-73 0.0022635961876101445 0.0 0.0 1.595588374499231 3.2925035456147556E-4 74-75 0.0023459087762505134 0.0 0.0 1.7431748459314123 4.1156294320184446E-4 76-77 0.002387065070570698 0.0 0.0 1.9095697438679178 4.1156294320184446E-4 78-79 0.002387065070570698 0.0 0.0 2.0998764488044506 4.1156294320184446E-4 80-81 0.002798628013772542 0.0 0.0 2.2910062796273873 4.5271923752202893E-4 82-83 0.002880940602412911 0.0 0.0 2.490614307080282 4.938755318422133E-4 84-85 0.00296325319105328 0.0 0.0 2.69771278009945 4.938755318422133E-4 86-87 0.003045565779693649 0.0 0.0 2.9106966032064046 4.938755318422133E-4 88-89 0.003045565779693649 0.0 0.0 3.1356569079605325 4.938755318422133E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2270 0.0 56.43193 1 GTATCAA 4115 0.0 46.290157 1 ATCAACG 5105 0.0 37.08375 3 TCAACGC 5165 0.0 36.745056 4 TATCAAC 5230 0.0 36.559715 2 CAACGCA 5295 0.0 35.932743 5 AACGCAG 5410 0.0 35.25248 6 TGGTATC 875 0.0 33.158905 2 GTGGTAT 970 0.0 32.401913 1 ACGCAGA 6285 0.0 30.334602 7 CGCAGAG 6335 0.0 30.01518 8 GCAGAGT 7080 0.0 26.38681 9 GAGTACT 4645 0.0 22.920662 12-13 CAGAGTA 6780 0.0 22.74882 10-11 GTGTAAG 555 0.0 21.450901 1 AGAGTAC 6450 0.0 21.444065 10-11 GTACTTT 4930 0.0 21.207813 14-15 GTATAGC 445 0.0 20.332561 1 AGTACTT 4930 0.0 20.294113 12-13 GTACATG 3950 0.0 20.253994 1 >>END_MODULE