FastQCFastQC Report
Thu 26 May 2016
SRR938208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938208_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214881
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59910.49313471854445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54440.4481097325581682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35520.29237431485059034No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA17710.14577559448209332No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16940.1394375251567849No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC16230.13359333136331872No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA16000.13170014182459022No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA15120.12445663402423776No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14600.12017637941493858No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG14280.11754237657844678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27250.059.434011
GTATCAA45950.042.6885761
TGGTATC8700.037.1224632
GTGGTAT9700.034.274681
AACCGAT702.0556862E-433.922157
TATCAAC60850.032.0015072
ATCAACG60800.031.8702773
TCAACGC61300.031.6090224
CAACGCA63650.030.4419965
AACGCAG65650.029.5869356
TAAGGTG6900.028.907575
ACGCAGA74500.025.7535137
CGCAGAG75900.025.278488
GTAAGGT7150.025.2399754
AGGTAAG6900.024.0916252
GCAGAGT82550.023.0119959
GTACATG40600.022.6946141
GAGTACT57150.022.64630912-13
TACATGG39450.022.6338272
GGTAAGG7850.021.7802123
Produced by FastQC (version 0.11.5)