##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938208_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1214881 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.660011145124503 31.0 31.0 34.0 30.0 34.0 2 31.957227909564804 31.0 31.0 34.0 30.0 34.0 3 32.00275253296413 31.0 31.0 34.0 30.0 34.0 4 35.704338943485 37.0 35.0 37.0 33.0 37.0 5 35.62359852528766 37.0 35.0 37.0 33.0 37.0 6 35.68426701874505 37.0 35.0 37.0 33.0 37.0 7 35.62258361106973 37.0 35.0 37.0 33.0 37.0 8 35.595228668486875 37.0 35.0 37.0 33.0 37.0 9 37.09020142713566 39.0 37.0 39.0 33.0 39.0 10-11 37.00085111216654 39.0 37.0 39.0 33.0 39.0 12-13 36.8549903241552 39.0 37.0 39.0 33.0 39.0 14-15 37.77049645191587 39.5 37.0 40.5 32.5 41.0 16-17 37.52938435945578 39.0 36.0 40.0 32.0 41.0 18-19 37.85852194577082 39.5 37.0 41.0 33.0 41.0 20-21 37.796416686078715 39.5 37.0 41.0 32.5 41.0 22-23 37.60411595868237 39.0 36.5 41.0 32.0 41.0 24-25 37.38799973001471 39.0 36.0 40.5 32.0 41.0 26-27 37.0548374696781 39.0 36.0 40.0 31.0 41.0 28-29 36.83092294636265 39.0 36.0 40.0 30.5 41.0 30-31 36.40374653978456 38.0 35.0 40.0 30.0 41.0 32-33 36.035653697769575 38.0 35.0 40.0 30.0 41.0 34-35 35.738404419856764 38.0 34.0 40.0 29.0 41.0 36-37 35.63955893622503 38.0 34.0 40.0 29.0 41.0 38-39 35.32291887024326 38.0 34.0 40.0 27.0 41.0 40-41 34.9802153461944 38.0 33.5 40.0 26.5 41.0 42-43 34.54456280080106 37.5 33.0 40.0 25.5 41.0 44-45 34.05303111992039 37.0 33.0 40.0 24.0 41.0 46-47 33.385003963351146 37.0 32.0 39.0 22.5 40.0 48-49 32.67987317276342 36.0 31.0 39.0 19.0 40.0 50-51 31.95517997235943 35.5 30.0 39.0 15.0 40.0 52-53 30.993214973318373 35.0 28.5 38.0 9.0 40.0 54-55 30.293949366234223 34.5 28.0 38.0 7.5 40.0 56-57 29.603601916566316 34.0 27.0 38.0 4.0 40.0 58-59 28.799753226859256 33.5 26.0 37.5 2.0 39.5 60-61 27.87261591876077 33.0 23.5 37.0 2.0 39.0 62-63 26.98911004452288 32.0 22.5 36.0 2.0 39.0 64-65 26.08940669909234 31.0 20.0 36.0 2.0 38.5 66-67 25.120345943347537 31.0 16.5 35.0 2.0 38.0 68-69 24.11156195545078 29.5 10.5 34.5 2.0 38.0 70-71 23.0729149603953 29.0 7.5 34.0 2.0 37.0 72-73 22.122565502300226 28.0 3.5 34.0 2.0 36.0 74-75 21.231461353004946 26.5 2.0 33.0 2.0 36.0 76-77 20.420872085414125 26.0 2.0 31.5 2.0 34.5 78-79 20.821295665995272 27.0 2.0 33.0 2.0 35.0 80-81 20.203012887682004 26.0 2.0 33.0 2.0 35.0 82-83 19.261202949095427 25.5 2.0 32.5 2.0 35.0 84-85 18.263154580572092 24.0 2.0 32.0 2.0 34.5 86-87 17.194474191299392 20.0 2.0 31.5 2.0 34.0 88-89 16.01264856393342 13.5 2.0 31.0 2.0 34.0 90-91 15.041336558889306 4.5 2.0 30.5 2.0 34.0 92-93 13.887874203317033 2.0 2.0 29.5 2.0 34.0 94-95 12.69943764039441 2.0 2.0 29.0 2.0 34.0 96-97 11.429658542688543 2.0 2.0 27.5 2.0 33.0 98-99 10.067816930217857 2.0 2.0 24.5 2.0 32.5 100-101 7.948663284716774 2.0 2.0 2.0 2.0 29.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.004308418121688362 1101 2 0.10155466621043274 1101 3 0.1356543169743034 1101 4 0.1919927198155591 1101 5 0.10641691515230178 1101 6 0.033126354654520185 1101 7 -0.0017202201523147664 1101 8 0.11310450744919365 1101 9 0.001757315980853491 1101 10-11 -0.031085555775241858 1101 12-13 0.12974899647601035 1101 14-15 0.039155390672299006 1101 16-17 0.23986071180549828 1101 18-19 0.23458833036465876 1101 20-21 0.2389584229928161 1101 22-23 0.1553874712581802 1101 24-25 0.40447910606773263 1101 26-27 0.32353112073856494 1101 28-29 0.41862349366049756 1101 30-31 0.44169115319692764 1101 32-33 0.4309332708084739 1101 34-35 0.44229932032409636 1101 36-37 0.6202126717333627 1101 38-39 0.37735240461393715 1101 40-41 0.3823630288887543 1101 42-43 0.4328391877955866 1101 44-45 0.48558013140199563 1101 46-47 0.2357471056062863 1101 48-49 0.3890916083241649 1101 50-51 0.19478597742192605 1101 52-53 -0.023876059210955702 1101 54-55 0.22955720370048738 1101 56-57 0.274606269041179 1101 58-59 0.27706213666735735 1101 60-61 0.09406320768614407 1101 62-63 0.14484923940807803 1101 64-65 0.09098932126054748 1101 66-67 -0.037913767306335444 1101 68-69 0.05598401477150716 1101 70-71 -0.3611911613411998 1101 72-73 -0.2708821306744369 1101 74-75 -0.23846364909012863 1101 76-77 -0.510569823559436 1101 78-79 -0.5906343267812204 1101 80-81 -0.6987112619081621 1101 82-83 -0.9345422935202734 1101 84-85 -0.8992495816025752 1101 86-87 -1.445725787522143 1101 88-89 -1.5017644782127189 1101 90-91 -1.3308055247503852 1101 92-93 -1.8363884488674262 1101 94-95 -2.0934366342251813 1101 96-97 -1.7831785590317786 1101 98-99 -1.7933408693718818 1101 100-101 -1.3567344043310792 1102 1 0.21092956139078467 1102 2 0.17931331493941371 1102 3 0.08043429864282103 1102 4 0.16580648512892537 1102 5 0.2634726367629483 1102 6 0.1260150217212157 1102 7 0.1462682077078057 1102 8 0.11891923980763153 1102 9 0.1487481670615196 1102 10-11 0.2888907790366986 1102 12-13 0.3328533856269118 1102 14-15 0.12363257258331828 1102 16-17 0.20322110620038103 1102 18-19 0.23993854461623698 1102 20-21 0.1675366611139566 1102 22-23 0.22583471091151353 1102 24-25 0.17576048440604808 1102 26-27 0.05493684412504507 1102 28-29 0.07975114797179828 1102 30-31 0.14386993316239227 1102 32-33 0.0924160898694879 1102 34-35 0.30134159676813965 1102 36-37 0.4063626796155262 1102 38-39 0.4648952191014146 1102 40-41 0.2990438709906087 1102 42-43 0.027308649546526453 1102 44-45 0.02511993758173503 1102 46-47 -0.11261468140826736 1102 48-49 0.21334207050566079 1102 50-51 0.45990805128497314 1102 52-53 0.16606854131904925 1102 54-55 -0.28608094946773477 1102 56-57 -0.0306821340820278 1102 58-59 -0.08247518959009525 1102 60-61 -0.35867152973183636 1102 62-63 -0.5797575714056258 1102 64-65 -0.5047138706735979 1102 66-67 -0.881865592011966 1102 68-69 -0.8736242011237998 1102 70-71 -0.7230897100881819 1102 72-73 -0.5711324906681803 1102 74-75 -0.9448766782778186 1102 76-77 -0.9668515352541363 1102 78-79 -1.4381892044082676 1102 80-81 -1.2549322840473884 1102 82-83 -1.3934319737411478 1102 84-85 -1.7604197310646228 1102 86-87 -1.858033429982152 1102 88-89 -2.0938895410675347 1102 90-91 -2.312783717634753 1102 92-93 -2.4043928404484003 1102 94-95 -2.1913219085338724 1102 96-97 -2.1631540588950102 1102 98-99 -1.7405154091687152 1102 100-101 -1.596494911132484 1103 1 0.10024286246879299 1103 2 0.25817562909584524 1103 3 0.1915323509173419 1103 4 0.13902281128642358 1103 5 0.16008582749378775 1103 6 0.07812173853380955 1103 7 0.011239837385318197 1103 8 0.009055247590694648 1103 9 0.11300613131948012 1103 10-11 0.25125194728022393 1103 12-13 0.1703622819593349 1103 14-15 0.46031223279238986 1103 16-17 0.3369076810634226 1103 18-19 0.27962108429877475 1103 20-21 0.34447736746642477 1103 22-23 0.3945254972493615 1103 24-25 0.27570955494335436 1103 26-27 0.2859411339528677 1103 28-29 0.3629516076428416 1103 30-31 0.39424374235973403 1103 32-33 0.3005543568312845 1103 34-35 0.41692535941072606 1103 36-37 0.31003417152231094 1103 38-39 0.400965684583646 1103 40-41 0.5320886040353372 1103 42-43 0.4561412548497117 1103 44-45 0.2894960843108194 1103 46-47 0.21158399573158704 1103 48-49 0.33479904490381074 1103 50-51 0.15575142359893945 1103 52-53 0.2220452443545753 1103 54-55 0.22052682184591887 1103 56-57 -0.07100455675738715 1103 58-59 -0.1651441546120047 1103 60-61 -0.2881276944821174 1103 62-63 -0.23384204901951833 1103 64-65 -0.12517837349104255 1103 66-67 -0.4467810686921503 1103 68-69 -0.49270120255374295 1103 70-71 -1.0918124905756663 1103 72-73 -1.2379787163379383 1103 74-75 -1.2734239480015574 1103 76-77 -1.146351773577905 1103 78-79 -1.2145990690534276 1103 80-81 -1.3214213505364576 1103 82-83 -1.4462810854137906 1103 84-85 -1.9147425324462475 1103 86-87 -1.9978968345514385 1103 88-89 -1.7610850906160262 1103 90-91 -2.0385971199187445 1103 92-93 -2.03156305585391 1103 94-95 -1.9567035856802555 1103 96-97 -1.9577157063978348 1103 98-99 -1.694388333629874 1103 100-101 -1.7210860063118147 1104 1 0.20592860846530314 1104 2 0.22626937774452927 1104 3 0.23310117055497415 1104 4 0.15831854330136252 1104 5 0.1507224938241265 1104 6 0.21087582129899118 1104 7 0.13876971039798747 1104 8 0.11415578523285319 1104 9 0.3259049306333779 1104 10-11 0.2987284884970549 1104 12-13 0.17821762306662237 1104 14-15 0.3897087186594632 1104 16-17 0.17395309957388605 1104 18-19 0.2409295871167103 1104 20-21 0.13190663076694875 1104 22-23 0.17032929389666407 1104 24-25 0.31413669938603306 1104 26-27 0.27358574239658395 1104 28-29 0.07498124282452778 1104 30-31 0.12023679481227134 1104 32-33 0.10569957786996298 1104 34-35 0.24495626302432072 1104 36-37 0.056806156474095815 1104 38-39 0.014844347541107084 1104 40-41 0.13609742540265302 1104 42-43 -0.1022285218774357 1104 44-45 0.08654698014085227 1104 46-47 -0.013359429322825633 1104 48-49 -0.37154599740127736 1104 50-51 -0.4575999752796527 1104 52-53 -0.36146514093161386 1104 54-55 -0.1529871669399867 1104 56-57 -0.43578395050459307 1104 58-59 -0.5296834111379418 1104 60-61 -0.9074882894919902 1104 62-63 -0.5389503702210696 1104 64-65 -0.5679778603526806 1104 66-67 -0.9298939158893482 1104 68-69 -0.626047124112759 1104 70-71 -0.7221637597282715 1104 72-73 -0.8654468974354153 1104 74-75 -1.426406636788908 1104 76-77 -1.3527074822421596 1104 78-79 -1.3565144012240324 1104 80-81 -1.4495566115019045 1104 82-83 -1.461864924438249 1104 84-85 -1.604238763185542 1104 86-87 -1.7890826742389425 1104 88-89 -1.8129166774531615 1104 90-91 -1.9925576543898202 1104 92-93 -1.7055146536456842 1104 94-95 -1.4352538256733354 1104 96-97 -1.3815789463765018 1104 98-99 -1.5111345175236721 1104 100-101 -1.4379155031191138 1105 1 0.1735812887483945 1105 2 0.2008799835649029 1105 3 0.127361227922691 1105 4 0.10235359814662814 1105 5 0.12876420793687515 1105 6 0.07201020301051386 1105 7 0.07464701843955623 1105 8 0.01440284117358459 1105 9 0.15273111222093405 1105 10-11 0.23559113750176408 1105 12-13 0.2627992567492541 1105 14-15 0.18109145357161083 1105 16-17 0.1795356413384397 1105 18-19 0.15509854801153722 1105 20-21 0.18672335677123897 1105 22-23 0.34410532918213477 1105 24-25 0.22108770620385343 1105 26-27 0.25958513700863506 1105 28-29 0.12421975126392937 1105 30-31 0.04741410141244273 1105 32-33 0.10154748135381908 1105 34-35 0.015473869723948042 1105 36-37 0.04647420207998465 1105 38-39 0.02854398863254204 1105 40-41 0.26203512809951235 1105 42-43 0.14292505928057864 1105 44-45 -0.02028237252645937 1105 46-47 -0.22004320060148785 1105 48-49 -0.38559820490520735 1105 50-51 -0.388557208944988 1105 52-53 -0.3952198434987473 1105 54-55 -0.41851061130915923 1105 56-57 -0.4270015009896255 1105 58-59 -0.3962365915867956 1105 60-61 -0.4244913308457541 1105 62-63 -0.7166533553602328 1105 64-65 -0.8497773039723242 1105 66-67 -0.7874991741161388 1105 68-69 -1.1240992163046961 1105 70-71 -1.130391558566501 1105 72-73 -1.3208664168726933 1105 74-75 -1.0541726111031622 1105 76-77 -1.3453586490553668 1105 78-79 -1.4709015900618283 1105 80-81 -1.4871967516059748 1105 82-83 -1.5138899471402958 1105 84-85 -1.6168051756853643 1105 86-87 -1.4608456504414313 1105 88-89 -1.6231935703715639 1105 90-91 -1.9431043773671774 1105 92-93 -1.844015309482634 1105 94-95 -1.8149923557337324 1105 96-97 -1.9512221999043273 1105 98-99 -1.8506144604442376 1105 100-101 -1.691292270635726 1106 1 0.2543559512738689 1106 2 0.13742869037762517 1106 3 0.15943141701001906 1106 4 0.08597730293753614 1106 5 0.1414651604247652 1106 6 0.19670985619557513 1106 7 0.19583106829428942 1106 8 0.23046473717990068 1106 9 0.12716345256341555 1106 10-11 0.1801963238855535 1106 12-13 0.055271387572467745 1106 14-15 0.1612222794643614 1106 16-17 0.24038866305297546 1106 18-19 0.25584564713750524 1106 20-21 0.25364914880124445 1106 22-23 0.28204490491698664 1106 24-25 0.1804530796498227 1106 26-27 0.2028576022820232 1106 28-29 0.15741145791557187 1106 30-31 0.08129689814841612 1106 32-33 0.43902267663354877 1106 34-35 0.32372778450866235 1106 36-37 0.35397019021515774 1106 38-39 0.1627979288151451 1106 40-41 0.15786833611429074 1106 42-43 0.10194355095308083 1106 44-45 -0.0434733239249141 1106 46-47 -0.1845606706770937 1106 48-49 -0.1697070763230215 1106 50-51 -0.07288811143244445 1106 52-53 -0.21337091686056908 1106 54-55 -0.35764455646441107 1106 56-57 -0.6349684863998704 1106 58-59 -0.33091354039065024 1106 60-61 -0.8247985785360505 1106 62-63 -1.1553438399052816 1106 64-65 -1.2534183595355692 1106 66-67 -1.7134766755915543 1106 68-69 -2.0317193867780396 1106 70-71 -2.1981247363043117 1106 72-73 -2.006317166010337 1106 74-75 -2.1429262227840518 1106 76-77 -1.8464317101099006 1106 78-79 -2.151099653539191 1106 80-81 -2.474890803218507 1106 82-83 -2.226811192947139 1106 84-85 -2.2254546960995896 1106 86-87 -2.1331590043203246 1106 88-89 -2.3212142723292732 1106 90-91 -2.4615181852668577 1106 92-93 -2.0092175870054323 1106 94-95 -2.0785252563671417 1106 96-97 -2.107173294252828 1106 98-99 -1.933379850064652 1106 100-101 -1.6479972146032908 1107 1 -0.1179306874389745 1107 2 -0.12034129873958932 1107 3 -0.045927821621738474 1107 4 0.02277378950476816 1107 5 -0.14199207129321678 1107 6 -0.031839319059955073 1107 7 -0.001625480582220007 1107 8 -0.1440137959912633 1107 9 -0.14274497732796476 1107 10-11 -0.08920064173764786 1107 12-13 -0.09108374600838687 1107 14-15 -0.19294372036493002 1107 16-17 -0.24237225707591392 1107 18-19 -0.25340572121278626 1107 20-21 -0.22321359472776692 1107 22-23 -0.3028201538713944 1107 24-25 -0.10568583857990888 1107 26-27 -0.18198058296006536 1107 28-29 -0.23726191180040956 1107 30-31 -0.5050358697068233 1107 32-33 -0.3343326356597274 1107 34-35 -0.28120141069864246 1107 36-37 -0.2796943612433438 1107 38-39 -0.5285947343732929 1107 40-41 -0.59515343745975 1107 42-43 -0.5757766120753303 1107 44-45 -0.565625995008709 1107 46-47 -0.6570159525625883 1107 48-49 -1.0096284295227704 1107 50-51 -0.9529600765221744 1107 52-53 -1.245106707889736 1107 54-55 -1.531222325706672 1107 56-57 -1.6352432233992147 1107 58-59 -1.571158042983317 1107 60-61 -1.5659696516641013 1107 62-63 -1.8782925814497808 1107 64-65 -2.0292669767815816 1107 66-67 -1.953039336966203 1107 68-69 -1.818565819464805 1107 70-71 -2.1132800160559277 1107 72-73 -2.230294170790227 1107 74-75 -2.038339448569854 1107 76-77 -2.44593084855601 1107 78-79 -2.3575875144315788 1107 80-81 -2.2580799663109232 1107 82-83 -2.3393329985293683 1107 84-85 -2.5464235307879353 1107 86-87 -2.807608330014368 1107 88-89 -2.724882278005394 1107 90-91 -3.23167034421955 1107 92-93 -3.161396211399941 1107 94-95 -2.843886188765836 1107 96-97 -2.8016494134726813 1107 98-99 -2.3330871751398714 1107 100-101 -1.9592909057882348 1108 1 -0.05153226618732276 1108 2 -0.08919646984944052 1108 3 -0.15926682710481543 1108 4 -0.1602393037075629 1108 5 -0.25717570714120797 1108 6 -0.28050636386993943 1108 7 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sequence quality scores pass #Quality Count 2 6.0 3 8.0 4 26.0 5 54.0 6 129.0 7 355.0 8 799.0 9 1590.0 10 2605.0 11 3983.0 12 6348.0 13 9356.0 14 12815.0 15 17501.0 16 22358.0 17 28797.0 18 30330.0 19 30390.0 20 32515.0 21 35192.0 22 37176.0 23 39779.0 24 41826.0 25 44848.0 26 49120.0 27 55082.0 28 61177.0 29 67078.0 30 72245.0 31 76468.0 32 81023.0 33 84628.0 34 85797.0 35 80929.0 36 61788.0 37 33630.0 38 6976.0 39 154.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.541938553505766 19.995340846698216 15.321617401196727 22.14110319859929 2 17.755450642716905 21.109185727280586 37.41299757566278 23.722366054339727 3 21.076616723754864 23.736671531717082 30.032260799629896 25.15445094489816 4 15.10872258270563 17.60493414581346 34.6352441103285 32.65109916115241 5 14.14211338615084 37.2045683712316 33.87953493157732 14.773783311040232 6 33.6250544301849 35.870088627569125 15.883324731512053 14.62153221073392 7 29.363616683444715 32.78115305120419 19.43490761646614 18.42032264888495 8 27.344900447039667 34.99058755548897 18.450202118561407 19.21430987890995 9 26.52780521532991 16.424008955617015 19.427597787435797 37.62058804161728 10-11 25.86652519876432 26.41452125763758 26.05107002249603 21.667883521102066 12-13 26.68723132358056 23.503633112252984 26.972200672089752 22.83693489207671 14-15 23.11909561512609 25.02208035190278 25.30054383927315 26.558280193697986 16-17 22.54162947085433 28.171974503942653 26.464239133163687 22.822156892039335 18-19 22.666870253135905 26.093831412294705 29.180676955191498 22.0586213793779 20-21 22.771613022180773 25.281447318708583 29.877988049858384 22.068951609252263 22-23 22.81696725852162 25.166168538317745 29.834238908996024 22.182625294164612 24-25 22.752534180412983 25.583934681137155 29.68887685255801 21.97465428589185 26-27 22.220539080895065 25.85155516770125 29.55431972733111 22.37358602407258 28-29 22.44314510253926 26.21684338942199 29.45889168861124 21.88111981942751 30-31 22.448242949965614 25.827921416456316 29.32716349428667 22.3966721392914 32-33 22.18834601907512 25.71881525844918 29.45169115328991 22.641147569185787 34-35 22.964580370721748 25.945994892131786 29.10250104441705 21.986923692729416 36-37 23.03375618943042 25.484175924489254 29.09969638814247 22.38237149793785 38-39 22.47611137611726 25.863383864678667 29.13681574737428 22.52368901182979 40-41 22.91755068376107 25.734126894300296 29.128217496397742 22.220104925540895 42-43 22.676469765091113 25.807792541325952 28.90479020524277 22.610947488340162 44-45 22.78820838757392 25.929422688075082 29.087881482993595 22.194487441357403 46-47 23.335103055445806 26.32449295403661 28.243941788489398 22.09646220202818 48-49 23.07096170918207 26.475513705747964 28.004602948372813 22.448921636697154 50-51 23.105914214157977 26.65287870088552 27.984919669665036 22.25628741529147 52-53 23.530021896968996 26.322708638601227 27.901801149179274 22.2454683152505 54-55 23.112218942266374 26.61209464053751 27.91981468340926 22.355871733786856 56-57 23.07786033301089 26.80478317245354 27.680284849856957 22.437071644678618 58-59 23.032298726271186 26.43865160361556 27.756881268352483 22.772168401760766 60-61 22.771996052164763 26.639121269168115 27.99867966477973 22.590203013887393 62-63 22.90529998970876 26.684120613358033 28.065616959967066 22.344962436966142 64-65 22.630704728031663 26.674117571992827 27.915578361088983 22.779599338886523 66-67 22.520985435636362 26.738900553752888 27.82936524433991 22.91074876627084 68-69 23.198914770809147 26.32306564895553 27.850208729590197 22.627810850645126 70-71 23.00374710981053 26.530961714277012 27.394941586991354 23.0703495889211 72-73 22.975581527438678 26.74594054418028 27.285362910328953 22.993115018052087 74-75 23.029043678923625 27.05054580645533 27.037291183082164 22.883119331538882 76-77 23.0356479082134 26.691905896668445 27.518732862770023 22.753713332348134 78-79 23.06477229781968 26.85243963387172 27.494119206584845 22.588668861723754 80-81 23.256047519888106 26.737922350869553 27.16517655930292 22.840853569939416 82-83 23.265134884548182 26.891850275763602 27.102028414602213 22.740986425086007 84-85 23.099238143304145 27.133740079540686 27.041734367365017 22.72528740979015 86-87 23.480448946756944 26.890242546853116 26.682459342915983 22.946849163473964 88-89 23.201750702701634 27.184879276929486 26.331345844468707 23.282024175900172 90-91 23.210945193343235 27.072169837730662 26.603014843675382 23.113870125250717 92-93 23.374392895633626 26.708804793767705 26.592550328221265 23.324251982377405 94-95 23.23145424167371 26.503519617944143 26.7247692109478 23.540256929434346 96-97 23.461872989959936 26.11141091485971 26.6603193342134 23.766396760966952 98-99 23.086187522381422 26.496931429488736 26.18690496281082 24.22997608531902 100-101 24.116587048023913 25.657358022109918 26.151822310617256 24.074232619248914 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 583.0 1 410.0 2 352.0 3 479.0 4 391.5 5 344.5 6 702.0 7 1560.0 8 2448.5 9 2776.5 10 2187.5 11 1467.0 12 1300.5 13 1325.5 14 1409.0 15 1534.5 16 1636.5 17 1735.5 18 1952.0 19 2217.5 20 2512.0 21 2923.0 22 3470.0 23 4269.0 24 5244.5 25 6386.5 26 7846.0 27 9420.5 28 10710.0 29 11852.0 30 13421.0 31 16140.5 32 19290.5 33 20451.5 34 20389.0 35 21240.5 36 23280.5 37 25387.0 38 26911.0 39 28209.5 40 29964.0 41 32143.0 42 33689.0 43 35029.5 44 36406.0 45 38593.5 46 40499.0 47 41719.0 48 42012.0 49 41666.0 50 42010.5 51 42075.5 52 42486.0 53 43596.0 54 44728.0 55 43436.5 56 40603.5 57 37892.5 58 35153.0 59 31727.0 60 26824.0 61 22606.0 62 19303.5 63 15708.0 64 11949.0 65 8913.0 66 6837.0 67 5265.0 68 4065.0 69 3232.5 70 2535.5 71 1854.0 72 1307.0 73 971.0 74 723.0 75 477.5 76 340.5 77 246.5 78 162.5 79 102.0 80 59.0 81 33.5 82 24.0 83 12.5 84 5.5 85 4.5 86 4.0 87 3.0 88 2.5 89 2.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005597256027545085 2 0.009548260282282792 3 0.007737383332194676 4 0.0 5 4.938755318422133E-4 6 0.0018108769500881157 7 0.0 8 0.0 9 8.231258864036889E-5 10-11 0.0 12-13 0.0011112199466449802 14-15 0.0 16-17 0.0021812835989697757 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00333365983993494 26-27 0.018191082089521523 28-29 0.006420381913948774 30-31 0.0038275353717771533 32-33 0.0 34-35 0.007284664094672646 36-37 6.173444148027667E-4 38-39 0.002016658421689038 40-41 0.0016050954784871934 42-43 0.0022224398932899603 44-45 4.1156294320184446E-5 46-47 8.231258864036889E-5 48-49 6.173444148027667E-4 50-51 0.004403723492259736 52-53 0.008313571452677259 54-55 0.007819695920835045 56-57 0.01695639325991599 58-59 0.024487995120509747 60-61 0.003127878368334018 62-63 0.021072022691934436 64-65 0.021689367106737203 66-67 0.013951983774542528 68-69 0.03218422215838424 70-71 0.017409112497438022 72-73 0.005185693084343241 74-75 0.017326799908797652 76-77 0.01954923980208761 78-79 0.02749240460588321 80-81 0.012141106824454411 82-83 0.035805976058560465 84-85 0.023253306290904212 86-87 0.03243115992430534 88-89 0.023294462585224395 90-91 0.029385594144611695 92-93 0.025023026946672144 94-95 0.015598235547349907 96-97 0.016256736256472854 98-99 0.012017637941493858 100-101 0.010865261700528693 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1214881.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.67307033221932 #Duplication Level Percentage of deduplicated Percentage of total 1 82.87072984163302 51.93763079847011 2 10.149579764062548 12.722106527911237 3 2.9518028148565856 5.549956362670492 4 1.255992105857759 3.148675263485423 5 0.7068163887371309 2.214917662164374 6 0.44053536698917956 1.6565822423485737 7 0.2970022732003235 1.3029831049979024 8 0.2198602906304736 1.102345556635668 9 0.15309710891841566 0.8635559287412975 >10 0.8673714284078822 9.703446025856774 >50 0.05393179224468252 2.3173848459494026 >100 0.027908733643882164 3.4991515813984733 >500 0.003668744948923465 1.5650124005981072 >1k 0.0014412926585056468 1.4772278347339356 >5k 2.6205321063739034E-4 0.9390238640381653 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5991 0.49313471854445 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5444 0.4481097325581682 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3552 0.29237431485059034 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1771 0.14577559448209332 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1694 0.1394375251567849 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1623 0.13359333136331872 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1600 0.13170014182459022 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1512 0.12445663402423776 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1460 0.12017637941493858 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1428 0.11754237657844678 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.231258864036889E-5 0.0 0.0 0.0 0.0 3 8.231258864036889E-5 0.0 0.0 0.0 0.0 4 8.231258864036889E-5 0.0 0.0 0.0 0.0 5 2.469377659211067E-4 0.0 0.0 0.0 0.0 6 2.469377659211067E-4 0.0 0.0 0.0 0.0 7 4.1156294320184446E-4 0.0 0.0 0.0 0.0 8 4.1156294320184446E-4 0.0 0.0 0.0 0.0 9 4.1156294320184446E-4 0.0 0.0 0.0 0.0 10-11 4.1156294320184446E-4 0.0 0.0 0.0 0.0 12-13 4.5271923752202893E-4 0.0 0.0 0.0 0.0 14-15 6.585007091229511E-4 0.0 0.0 0.0 0.0 16-17 6.585007091229511E-4 0.0 0.0 0.0 0.0 18-19 7.4081329776332E-4 0.0 0.0 0.0 0.0 20-21 8.231258864036889E-4 0.0 0.0 4.1156294320184446E-5 0.0 22-23 9.054384750440579E-4 0.0 0.0 8.231258864036889E-5 0.0 24-25 0.0012346888296055334 0.0 0.0 3.2925035456147556E-4 0.0 26-27 0.0012346888296055334 0.0 0.0 5.761881204825823E-4 0.0 28-29 0.0018931895387284845 0.0 0.0 0.0014404703012064555 0.0 30-31 0.002140127304649591 0.0 0.0 0.004691817552501027 0.0 32-33 0.002140127304649591 0.0 0.0 0.010700636523247955 4.1156294320184446E-5 34-35 0.002140127304649591 0.0 0.0 0.02296521223066292 8.231258864036889E-5 36-37 0.002140127304649591 0.0 0.0 0.046053893344286397 8.231258864036889E-5 38-39 0.00222243989328996 0.0 0.0 0.08009014874707893 8.231258864036889E-5 40-41 0.0022635961876101445 0.0 0.0 0.1145791233873935 8.231258864036889E-5 42-43 0.002387065070570698 0.0 0.0 0.15133169421531822 8.231258864036889E-5 44-45 0.0026340028364918045 0.0 0.0 0.1987025889778505 1.2346888296055334E-4 46-47 0.002880940602412911 0.0 0.0 0.25916118533420146 1.6462517728073778E-4 48-49 0.0029632531910532803 0.0 0.0 0.32089562681447814 1.6462517728073778E-4 50-51 0.0029632531910532803 0.0 0.0 0.38514060224828606 1.6462517728073778E-4 52-53 0.0029632531910532803 0.0 0.0 0.44773932590928656 1.6462517728073778E-4 54-55 0.0029632531910532803 0.0 0.0 0.5187750899059249 1.6462517728073778E-4 56-57 0.0032925035456147556 0.0 0.0 0.5977128624120387 1.6462517728073778E-4 58-59 0.0032925035456147556 0.0 0.0 0.6805193265842499 1.6462517728073778E-4 60-61 0.0033336598399349403 0.0 0.0 0.7671533261282382 1.6462517728073778E-4 62-63 0.0033748161342551245 0.0 0.0 0.8531288249631034 1.6462517728073778E-4 64-65 0.0033748161342551245 0.0 0.0 0.9450308301800752 1.6462517728073778E-4 66-67 0.0033748161342551245 0.0 0.0 1.052078351706875 1.6462517728073778E-4 68-69 0.0033748161342551245 0.0 0.0 1.1685095083386767 1.6462517728073778E-4 70-71 0.0033748161342551245 0.0 0.0 1.2914022031787478 1.6462517728073778E-4 72-73 0.0033748161342551245 0.0 0.0 1.4203037169895651 2.0578147160092223E-4 74-75 0.0033748161342551245 0.0 0.0 1.5484644175026196 2.469377659211067E-4 76-77 0.0033748161342551245 0.0 0.0 1.6900420699640541 2.469377659211067E-4 78-79 0.0034159724285753087 0.0 0.0 1.8477529897990008 2.469377659211067E-4 80-81 0.003539441311535862 0.0 0.0 2.006410504403312 2.469377659211067E-4 82-83 0.0036217539001762314 0.0 0.0 2.168360522553238 2.469377659211067E-4 84-85 0.003745222783136785 0.0 0.0 2.333726513131739 2.469377659211067E-4 86-87 0.003868691666097338 0.0 0.0 2.495511906104384 2.469377659211067E-4 88-89 0.003868691666097338 0.0 0.0 2.6638823061682584 2.8809406024129115E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2725 0.0 59.43401 1 GTATCAA 4595 0.0 42.688576 1 TGGTATC 870 0.0 37.122463 2 GTGGTAT 970 0.0 34.27468 1 AACCGAT 70 2.0556862E-4 33.92215 7 TATCAAC 6085 0.0 32.001507 2 ATCAACG 6080 0.0 31.870277 3 TCAACGC 6130 0.0 31.609022 4 CAACGCA 6365 0.0 30.441996 5 AACGCAG 6565 0.0 29.586935 6 TAAGGTG 690 0.0 28.90757 5 ACGCAGA 7450 0.0 25.753513 7 CGCAGAG 7590 0.0 25.27848 8 GTAAGGT 715 0.0 25.239975 4 AGGTAAG 690 0.0 24.091625 2 GCAGAGT 8255 0.0 23.011995 9 GTACATG 4060 0.0 22.694614 1 GAGTACT 5715 0.0 22.646309 12-13 TACATGG 3945 0.0 22.633827 2 GGTAAGG 785 0.0 21.780212 3 >>END_MODULE