FastQCFastQC Report
Thu 26 May 2016
SRR938207_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938207_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227754
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101970.45772558370448446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83660.37553518027573957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49230.22098490228274753No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33520.15046544636436518No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31280.14041047620159136No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA31000.13915360493124465No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28260.12685422178570885No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA27080.1215574071463905No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA25030.11235531391706624No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA23950.1075073818743003No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG23140.10387143284222584No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22580.1013576903015324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45200.055.1996081
GTATCAA82350.044.201161
TATCAAC103400.035.0184332
ATCAACG103200.034.8100283
TGGTATC19350.034.625852
TCAACGC104450.034.438944
CAACGCA105850.033.8936545
AACGCAG110000.032.695466
GTGGTAT22050.031.3712751
ACGCAGA126350.028.1204877
TAAGGTG17000.027.952035
CGCAGAG127250.027.7722858
GTAAGGT15650.026.7196434
GCAGAGT138050.024.8076489
AGGTAAG15750.024.7397652
GGTAAGG17900.023.6264933
GAGTACT89950.022.99765212-13
AAGGTAA16550.022.483781
GTACATG79650.022.4005431
GTACTTT92900.022.11447314-15
Produced by FastQC (version 0.11.5)