FastQCFastQC Report
Thu 26 May 2016
SRR938207_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938207_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227754
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98580.44250846368135804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86130.38662258041058395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51230.2299625542137956No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34900.15666002619678834No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31380.14085935879814376No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA31310.1405451409805571No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC30920.13879449885400272No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA29930.13435056114813396No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA24970.11208598435913482No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA24860.11159221350292717No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24370.10939268877982039No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG23490.10544252193015927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49500.055.2477571
GTATCAA85000.041.9656871
TATCAAC109400.032.6058772
ATCAACG110100.032.3092773
TCAACGC111000.032.0473064
CAACGCA113450.031.3538275
AACGCAG116400.030.5207966
GTGGTAT21900.029.9700151
TGGTATC21800.029.8893222
ACGCAGA131950.026.7080237
CGCAGAG133350.026.4276268
GCAGAGT146600.023.7150679
GAGTACT94300.023.09270912-13
TAAGGTG18650.022.9486475
GTAAGGT17900.022.3171734
CAGAGTA138750.021.82199310-11
GTACTTT100150.021.5066914-15
GTACATG80200.021.052611
CGTATAG1150.003696717620.6787451
TACATGG79150.020.5507262
Produced by FastQC (version 0.11.5)