##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938207_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2227754 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49477321104575 33.0 31.0 34.0 31.0 34.0 2 32.72691149920503 34.0 31.0 34.0 31.0 34.0 3 32.78597322684641 34.0 31.0 34.0 31.0 34.0 4 36.24018630423287 37.0 35.0 37.0 35.0 37.0 5 36.165963566892934 37.0 35.0 37.0 35.0 37.0 6 36.22002204911314 37.0 37.0 37.0 35.0 37.0 7 36.19342036867626 37.0 37.0 37.0 35.0 37.0 8 36.18369218504377 37.0 37.0 37.0 35.0 37.0 9 37.87844079732322 39.0 38.0 39.0 35.0 39.0 10-11 37.85063229602551 39.0 38.0 39.0 35.0 39.0 12-13 37.77241921684352 39.0 38.0 39.0 35.0 39.0 14-15 39.02865621608131 40.0 38.0 41.0 36.0 41.0 16-17 38.93985870971391 40.0 38.0 41.0 35.5 41.0 18-19 39.18727314595777 40.0 39.0 41.0 36.0 41.0 20-21 39.15676124922231 40.0 39.0 41.0 36.0 41.0 22-23 39.05925564492309 40.0 39.0 41.0 35.5 41.0 24-25 38.95624988216832 40.0 38.0 41.0 35.0 41.0 26-27 38.79393258860718 40.0 38.0 41.0 35.0 41.0 28-29 38.675442171801734 40.0 38.0 41.0 34.5 41.0 30-31 38.42823983258475 40.0 38.0 41.0 34.0 41.0 32-33 38.19052238263291 40.0 38.0 41.0 34.0 41.0 34-35 38.08898626149925 40.0 38.0 41.0 34.0 41.0 36-37 38.02737488070945 40.0 38.0 41.0 33.0 41.0 38-39 37.832077285014414 40.0 37.0 41.0 33.0 41.0 40-41 37.60235780072665 40.0 37.0 41.0 33.0 41.0 42-43 37.34089715471277 40.0 37.0 41.0 32.0 41.0 44-45 37.063084164589085 40.0 36.0 41.0 31.0 41.0 46-47 36.68732903184104 39.5 36.0 41.0 30.5 41.0 48-49 36.26048769298585 39.0 35.5 41.0 30.0 41.0 50-51 35.870466622436766 39.0 35.0 40.0 28.5 41.0 52-53 35.328244276522454 38.0 35.0 40.0 27.0 41.0 54-55 34.94267544800728 38.0 34.0 40.0 26.0 41.0 56-57 34.56113219861798 38.0 34.0 40.0 26.0 41.0 58-59 34.049161397533126 37.5 33.5 40.0 23.5 41.0 60-61 33.568569958801554 37.0 33.0 40.0 22.5 41.0 62-63 33.007236885221616 36.0 32.0 39.0 21.0 40.5 64-65 32.435412303153754 35.5 31.5 39.0 18.5 40.0 66-67 31.869442272351435 35.0 31.0 38.5 15.5 40.0 68-69 31.25175670204161 35.0 30.5 38.0 9.5 40.0 70-71 30.636605298430617 34.5 29.5 37.0 7.5 39.5 72-73 29.971056947939495 34.0 29.0 36.5 4.0 39.0 74-75 29.328692934677708 34.0 28.0 36.0 2.0 39.0 76-77 28.05293178690286 32.5 26.5 34.5 2.0 36.5 78-79 28.69669923160277 33.5 28.0 35.0 2.0 37.0 80-81 28.42290239407044 34.0 27.5 35.0 2.0 37.0 82-83 27.87236517137889 33.5 26.5 35.0 2.0 36.0 84-85 27.22617578062928 33.0 26.0 35.0 2.0 36.0 86-87 26.53028229328732 32.5 24.5 35.0 2.0 35.5 88-89 25.739431732588066 32.0 22.0 34.5 2.0 35.0 90-91 25.093506284805233 31.5 19.0 34.0 2.0 35.0 92-93 24.175887014454915 31.0 11.5 34.0 2.0 35.0 94-95 23.28724289127076 31.0 2.0 34.0 2.0 35.0 96-97 22.25061945798324 30.0 2.0 34.0 2.0 35.0 98-99 20.854294953572072 29.5 2.0 34.0 2.0 35.0 100-101 16.998763552887798 22.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13334373471992222 1101 2 0.1870132144623966 1101 3 0.1570135614432857 1101 4 0.05979193019386031 1101 5 -0.09179926968051433 1101 6 0.018698132629111797 1101 7 0.08107419400818117 1101 8 0.048801937866990386 1101 9 0.04497759237265342 1101 10-11 0.10033784509673183 1101 12-13 0.17587216402337447 1101 14-15 0.30933051768993636 1101 16-17 0.3483631855937901 1101 18-19 0.2755351871583258 1101 20-21 0.23840451010438102 1101 22-23 0.2656778812588172 1101 24-25 0.2877335137885453 1101 26-27 0.34716101998697724 1101 28-29 0.40661721645660975 1101 30-31 0.5115547549268342 1101 32-33 0.5023021444416358 1101 34-35 0.23973074289855134 1101 36-37 0.22717507115414293 1101 38-39 0.37066480456909545 1101 40-41 0.4402034122113321 1101 42-43 0.4220486215013324 1101 44-45 0.48059762301172526 1101 46-47 0.6297395539431179 1101 48-49 0.8048727182256741 1101 50-51 0.6587066466147391 1101 52-53 0.6632168326053005 1101 54-55 0.7484035933958921 1101 56-57 0.7229191828396822 1101 58-59 0.9370921507712708 1101 60-61 0.865024572417326 1101 62-63 1.0095155561158151 1101 64-65 1.106693426188123 1101 66-67 1.3393783529266052 1101 68-69 1.5853134271898348 1101 70-71 1.5408331524285828 1101 72-73 1.7685208717134984 1101 74-75 1.5548478144207039 1101 76-77 1.6857085615166305 1101 78-79 1.9027428637224482 1101 80-81 1.8620203585585315 1101 82-83 1.969427852343415 1101 84-85 2.1367728624672324 1101 86-87 2.2951951087676115 1101 88-89 2.638462165677641 1101 90-91 2.796937490756086 1101 92-93 3.0012282025965717 1101 94-95 3.0453183047735344 1101 96-97 3.393330912415898 1101 98-99 3.64346482053708 1101 100-101 3.8787101410810756 1102 1 0.14503357515120285 1102 2 0.1090823896089006 1102 3 0.14951765135606365 1102 4 0.07894705331887053 1102 5 -0.04158796955921673 1102 6 0.008468583204304991 1102 7 0.05349740448713902 1102 8 0.047782565751667505 1102 9 0.08718300741139728 1102 10-11 0.1542507257648964 1102 12-13 0.18807024582394405 1102 14-15 0.19307138798176737 1102 16-17 0.2555790861807381 1102 18-19 0.23895755567290422 1102 20-21 0.16812316571205344 1102 22-23 0.19011793449269732 1102 24-25 0.30195054758905826 1102 26-27 0.3414471330066817 1102 28-29 0.2275213660061297 1102 30-31 0.29709755613135513 1102 32-33 0.16596121069908776 1102 34-35 0.2769056085569801 1102 36-37 0.36240946028499366 1102 38-39 0.43951330703402647 1102 40-41 0.3529917647869567 1102 42-43 0.4560161345049778 1102 44-45 0.34224747446886994 1102 46-47 0.3835659555653237 1102 48-49 0.3913721342826335 1102 50-51 0.36088428318546306 1102 52-53 0.5850708936532101 1102 54-55 0.6980099736020478 1102 56-57 0.8119431918268063 1102 58-59 0.8634927905948544 1102 60-61 0.6632869879247281 1102 62-63 0.9196440929296514 1102 64-65 0.8731596953150742 1102 66-67 0.8276811445846342 1102 68-69 1.0341630796267118 1102 70-71 1.0556273392337232 1102 72-73 1.4451090320877782 1102 74-75 1.4086768453757514 1102 76-77 1.3832488928819124 1102 78-79 1.3696960506183125 1102 80-81 1.5742510036350055 1102 82-83 1.7425896741994684 1102 84-85 1.9848511668885003 1102 86-87 2.1942469308952646 1102 88-89 2.378976775233273 1102 90-91 2.3255486771515166 1102 92-93 2.6337668032251855 1102 94-95 2.7992891752329534 1102 96-97 2.997966512889061 1102 98-99 3.476039018287178 1102 100-101 3.5136093625096194 1103 1 0.15719388049057414 1103 2 0.16999756148435097 1103 3 0.0916546255301327 1103 4 -0.00923211223801701 1103 5 -0.10773678670005182 1103 6 0.01766000996391881 1103 7 0.11607304362599535 1103 8 0.09062083103260221 1103 9 0.04783465266267939 1103 10-11 0.12283420777722398 1103 12-13 0.15616700642101478 1103 14-15 0.22619678770630713 1103 16-17 0.23299041175003055 1103 18-19 0.23654890171460607 1103 20-21 0.15707693572134218 1103 22-23 0.21482070759778793 1103 24-25 0.2991678680906986 1103 26-27 0.23283109259998724 1103 28-29 0.1570598635138012 1103 30-31 0.25822086955825085 1103 32-33 0.29509413867082657 1103 34-35 0.30220983317409633 1103 36-37 0.37565495502666835 1103 38-39 0.3704322273017553 1103 40-41 0.4051751083637214 1103 42-43 0.4646408271340974 1103 44-45 0.5955777664149267 1103 46-47 0.5732432846269759 1103 48-49 0.6175263109192457 1103 50-51 0.7126909702956041 1103 52-53 0.9215262598483562 1103 54-55 0.8741050764441454 1103 56-57 0.8441411221283985 1103 58-59 1.0008986264782038 1103 60-61 1.0276096979201341 1103 62-63 0.9548979664943786 1103 64-65 1.1990577227711015 1103 66-67 1.4107595673522901 1103 68-69 1.396765787473985 1103 70-71 1.454496691759985 1103 72-73 1.617111959197004 1103 74-75 1.8400164426649752 1103 76-77 1.9746509434857522 1103 78-79 1.9658485510563501 1103 80-81 2.1390960203617304 1103 82-83 2.2754220104025933 1103 84-85 2.46865089750332 1103 86-87 2.61062184498331 1103 88-89 2.6985765814668845 1103 90-91 2.895166791379296 1103 92-93 2.919607753136141 1103 94-95 3.086400564324208 1103 96-97 3.126431131266383 1103 98-99 3.6663449218151705 1103 100-101 3.832084651057169 1104 1 0.219707151798886 1104 2 0.16183753089115527 1104 3 0.15999066763603054 1104 4 0.16203896461229306 1104 5 0.056117452822533664 1104 6 0.09995319140471537 1104 7 0.17016010340852716 1104 8 0.16131929181243265 1104 9 0.06593847832171917 1104 10-11 0.21879922687281095 1104 12-13 0.1590089533156558 1104 14-15 0.379753498333919 1104 16-17 0.3672598230825983 1104 18-19 0.2578818694055798 1104 20-21 0.2409251874308822 1104 22-23 0.2899018449803563 1104 24-25 0.27065053110764836 1104 26-27 0.39545028278601535 1104 28-29 0.31168456852595483 1104 30-31 0.2631092396151615 1104 32-33 0.20307000627474991 1104 34-35 0.33245709463019324 1104 36-37 0.37848432248214436 1104 38-39 0.42887730798627643 1104 40-41 0.42013608694345805 1104 42-43 0.4876805731300635 1104 44-45 0.5719817576193762 1104 46-47 0.5077240305373962 1104 48-49 0.6401836419648035 1104 50-51 0.6524613703720874 1104 52-53 0.8088975092885704 1104 54-55 0.9645600495996263 1104 56-57 0.799995089935102 1104 58-59 1.0496502429361314 1104 60-61 1.061057420286879 1104 62-63 1.0590199971177654 1104 64-65 1.2350968096044568 1104 66-67 1.529143411326988 1104 68-69 1.5818739958096195 1104 70-71 1.6729145002204149 1104 72-73 1.9349574031757335 1104 74-75 1.733506079723 1104 76-77 1.785855701532821 1104 78-79 1.8725445865203056 1104 80-81 2.032376335552158 1104 82-83 2.2347079008004513 1104 84-85 2.413574590625384 1104 86-87 2.5685295320269894 1104 88-89 2.6979825803498336 1104 90-91 3.1940472212942055 1104 92-93 3.406892959212513 1104 94-95 3.5693589465076982 1104 96-97 3.8915360186300845 1104 98-99 4.1276908211695975 1104 100-101 3.7484794341121592 1105 1 0.13249861321103396 1105 2 0.12080304813252951 1105 3 0.12462120950794997 1105 4 0.09688297118043465 1105 5 -0.003274993811295701 1105 6 0.01318800257055841 1105 7 0.04856732836747568 1105 8 0.05424211611456542 1105 9 0.13754767372401489 1105 10-11 0.07883206759530736 1105 12-13 0.17899253295441042 1105 14-15 0.2545318234570786 1105 16-17 0.1987212352336698 1105 18-19 0.20131372490639876 1105 20-21 0.16640958808769568 1105 22-23 0.19954880721352453 1105 24-25 0.24644691862817325 1105 26-27 0.20393960955120605 1105 28-29 0.28800640760641016 1105 30-31 0.33263304913896974 1105 32-33 0.3748105645670279 1105 34-35 0.34703837541837146 1105 36-37 0.423681366817128 1105 38-39 0.44675087120467083 1105 40-41 0.3884201591930463 1105 42-43 0.31474953864730537 1105 44-45 0.42919029620723137 1105 46-47 0.4542182834109596 1105 48-49 0.4857419342472298 1105 50-51 0.5837175280075613 1105 52-53 0.8316561299782279 1105 54-55 0.8447899346571006 1105 56-57 0.6699047779482399 1105 58-59 0.8209392412940986 1105 60-61 0.836205203538114 1105 62-63 1.0771480759354972 1105 64-65 1.1438899130527318 1105 66-67 1.214438977829456 1105 68-69 1.5243206296355645 1105 70-71 1.7075860929954558 1105 72-73 1.619579734867031 1105 74-75 1.7821349795587942 1105 76-77 1.520166046360405 1105 78-79 1.701107804911107 1105 80-81 1.8735668117426343 1105 82-83 1.9778277694375674 1105 84-85 2.125249467472674 1105 86-87 2.1025032594489907 1105 88-89 2.0933766690197864 1105 90-91 2.3349503411628447 1105 92-93 2.628740249852907 1105 94-95 2.9686200302515395 1105 96-97 3.270797102373926 1105 98-99 3.4454822825817466 1105 100-101 3.3179375621909806 1106 1 0.1303113578485835 1106 2 0.11141768947991437 1106 3 0.06222303464382861 1106 4 0.055993664776806895 1106 5 -0.05108706744299951 1106 6 -0.020207063299842787 1106 7 0.09864337569575099 1106 8 0.04945359478776368 1106 9 0.12482294268552607 1106 10-11 0.11363803507389747 1106 12-13 0.10236321894604572 1106 14-15 0.036124120310056185 1106 16-17 0.0632963767040664 1106 18-19 -0.029285259638101024 1106 20-21 -0.03488366383026431 1106 22-23 -0.02532258333423698 1106 24-25 -0.015264593595318843 1106 26-27 -0.11218287138104444 1106 28-29 -0.02674856342850518 1106 30-31 0.0814679593360168 1106 32-33 0.1053706307729314 1106 34-35 0.12420002072467895 1106 36-37 0.246141609721235 1106 38-39 0.1274911024780181 1106 40-41 0.27847506983743386 1106 42-43 0.240013243941533 1106 44-45 0.3156283735518315 1106 46-47 0.45393388877005947 1106 48-49 0.4774014435182963 1106 50-51 0.6360965792053079 1106 52-53 0.666647742405253 1106 54-55 0.7138977205615049 1106 56-57 0.7985673514792992 1106 58-59 0.9425877908031453 1106 60-61 0.863394024580856 1106 62-63 0.8998830957301678 1106 64-65 0.8535332734499619 1106 66-67 1.0245198340329047 1106 68-69 1.0238867125271476 1106 70-71 1.0547418110835345 1106 72-73 1.4859939915043938 1106 74-75 1.8395112348988825 1106 76-77 1.7732751519096261 1106 78-79 1.7917507403379815 1106 80-81 1.6968298012973122 1106 82-83 2.0694366177678774 1106 84-85 2.1361419052368795 1106 86-87 2.1676615049923953 1106 88-89 2.241412970435608 1106 90-91 2.221717882373788 1106 92-93 2.2842502142895995 1106 94-95 2.5153992022303377 1106 96-97 2.9998221308916726 1106 98-99 3.206922149275254 1106 100-101 3.2795862235343343 1107 1 0.14878969302323952 1107 2 0.10491438280724452 1107 3 0.10735762801901672 1107 4 0.06852616366870024 1107 5 0.007073765410702038 1107 6 0.05715849217485669 1107 7 0.045918099661903966 1107 8 0.09112340384092477 1107 9 0.14265163079980425 1107 10-11 0.23289645391322011 1107 12-13 0.16015953496648194 1107 14-15 0.3304473164148263 1107 16-17 0.2776011815783974 1107 18-19 0.15889253107675216 1107 20-21 0.18007155991868018 1107 22-23 0.20399213383995374 1107 24-25 0.2685528877763943 1107 26-27 0.2635439019723975 1107 28-29 0.2668326986862226 1107 30-31 0.3483540417613753 1107 32-33 0.3685999676522087 1107 34-35 0.3952140964109887 1107 36-37 0.47205494159914707 1107 38-39 0.5012009047392141 1107 40-41 0.5916629492668193 1107 42-43 0.4404269363669542 1107 44-45 0.6504109534842186 1107 46-47 0.8369580553760798 1107 48-49 0.8690719141265006 1107 50-51 0.9774029740169468 1107 52-53 1.003259081017795 1107 54-55 1.032120585227183 1107 56-57 1.1629463607785553 1107 58-59 1.061827906619385 1107 60-61 1.1364164711437468 1107 62-63 1.204840899543811 1107 64-65 1.1458718044008123 1107 66-67 1.3506193936577553 1107 68-69 1.4937035940889452 1107 70-71 1.6393868978244228 1107 72-73 1.7547809421104894 1107 74-75 1.648342223019558 1107 76-77 1.7339118545428356 1107 78-79 1.7530159202699274 1107 80-81 1.7749316675441094 1107 82-83 1.9457419210136884 1107 84-85 2.049099232730889 1107 86-87 2.119431495532158 1107 88-89 2.300549707249157 1107 90-91 2.514735720103154 1107 92-93 2.8471540660836254 1107 94-95 2.892932572169716 1107 96-97 3.31564619694133 1107 98-99 3.695371618597843 1107 100-101 3.382961036369384 1108 1 0.21072644122943984 1108 2 0.1794125538101028 1108 3 0.13607969866277614 1108 4 0.13336718504685052 1108 5 0.08469966678116947 1108 6 0.13444228057876728 1108 7 0.11841275344850288 1108 8 0.1788265543639227 1108 9 0.19702524676179678 1108 10-11 0.1804766359414458 1108 12-13 0.1725810735586606 1108 14-15 0.2976969829049594 1108 16-17 0.3361956805091566 1108 18-19 0.3151110096460883 1108 20-21 0.3611517743482082 1108 22-23 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54-55 0.01731318114666891 2316 56-57 0.17970882497012752 2316 58-59 0.22607675814246875 2316 60-61 0.3499690874506882 2316 62-63 0.3673860858653697 2316 64-65 0.5453530343098265 2316 66-67 0.6552776429264533 2316 68-69 0.9208741776043787 2316 70-71 1.0522878554632484 2316 72-73 1.04304435754878 2316 74-75 1.3777495399655422 2316 76-77 1.4522034625450821 2316 78-79 1.5438394593786988 2316 80-81 1.6428410947270606 2316 82-83 1.9046411662402107 2316 84-85 1.9684991987517826 2316 86-87 2.1840688358766265 2316 88-89 2.4061736695828166 2316 90-91 2.6727147539863765 2316 92-93 2.702632607942686 2316 94-95 3.1887075564838163 2316 96-97 3.630020115118974 2316 98-99 3.807623945409542 2316 100-101 4.216235663230034 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 7.0 4 16.0 5 32.0 6 77.0 7 189.0 8 515.0 9 1042.0 10 1902.0 11 3085.0 12 4749.0 13 7085.0 14 9483.0 15 13603.0 16 17793.0 17 25031.0 18 25961.0 19 21451.0 20 20099.0 21 21074.0 22 22934.0 23 25200.0 24 28329.0 25 31568.0 26 36083.0 27 41708.0 28 49676.0 29 59220.0 30 71444.0 31 85313.0 32 103926.0 33 129479.0 34 169536.0 35 233437.0 36 325618.0 37 407029.0 38 223160.0 39 10895.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09578167068716 18.781696722349057 15.137398473978724 22.98512313298506 2 15.93717703405133 19.716512780360258 39.739077184989505 24.607233000598903 3 19.595804794751146 23.363750546531243 29.70485966155015 27.335584997167466 4 13.100341022384839 16.819312002937544 36.53316203658191 33.54718493809571 5 13.671119428205735 37.116832409344866 34.23179527004192 14.980252892407474 6 32.67236867266314 35.98866840773263 16.786054474596387 14.552908445007843 7 29.049302571109735 32.67075269531555 19.822296357676837 18.45764837589788 8 26.825619200709593 34.5932736647857 19.208969045463338 19.372138089041368 9 26.06961989519489 16.81226921823505 20.061191675562025 37.05691921100804 10-11 25.15578470513351 26.43864627782062 26.69848196883498 21.707087048210887 12-13 26.30144531218438 23.619775792120674 27.26806909560032 22.810709800094624 14-15 22.655104647999735 25.28438956904577 25.73663878507232 26.323866997882174 16-17 22.110565170122015 28.395549957490818 26.92958917366998 22.56429569871718 18-19 22.141246295596375 26.516617184841774 29.48644688776229 21.85568963179956 20-21 22.427072288951113 25.687935023346387 30.038033822405886 21.846958865296617 22-23 22.292986196835372 25.782830209852992 29.81279317697228 22.111390416339354 24-25 22.25732255230747 25.843174767623346 29.81432760810166 22.085175071967523 26-27 21.50596041057364 26.3417601335958 29.737275067729385 22.415004388101174 28-29 21.80117284044827 26.736928763229695 29.463419210559156 21.998479185762882 30-31 21.907041800845157 26.172324233286083 29.455137326652764 22.465496639216 32-33 21.499927729901955 26.188843112839212 29.57005127137018 22.741177885888657 34-35 22.432686989563205 26.386170350311378 29.24971178502934 21.931430875096073 36-37 22.459497693518127 25.83957713264566 29.224459638194432 22.476465535641783 38-39 21.94584623865624 26.307084099198175 29.14064746320201 22.60642219894357 40-41 22.599950528096855 25.993265177032647 29.199197136555473 22.20758715831503 42-43 22.064289300731023 26.12651497654349 29.066989700056133 22.742206022669347 44-45 22.154500923832728 26.42206496815515 29.22415557738199 22.199278530630128 46-47 22.655284201038356 26.640710778658683 28.346935972284193 22.357069048018765 48-49 22.358887022534805 26.696619106059288 28.230652935647292 22.71384093575862 50-51 22.600969406855516 27.083488571897973 28.107098000946245 22.208444020300266 52-53 22.948224983548453 26.640665890399028 28.130888778563524 22.280220347488996 54-55 22.40615435995177 26.91071366048495 28.34985370916178 22.33327827040149 56-57 22.47614524590473 27.101680482531055 27.994216594029947 22.42795767753427 58-59 22.4637908853491 26.69197317118497 27.90954477020353 22.934691173262397 60-61 22.09788423676941 27.02214876507909 28.206570384342257 22.673396613809242 62-63 22.278985920348475 26.982332878764893 28.390769357837538 22.347911843049097 64-65 21.928296391791914 27.064433505674323 28.13504094258163 22.87222915995213 66-67 21.875669817149877 27.04848180016326 28.136887676307097 22.938960706379767 68-69 22.55517881506975 26.59381088724891 28.321709790821124 22.52930050686022 70-71 22.272050684231743 26.93369644942844 27.7782017224523 23.01605114388752 72-73 22.30699377668609 26.962404253256196 27.79490266421038 22.93569930584733 74-75 22.262422152535695 27.429711718618844 27.55535395739386 22.752512171451606 76-77 22.35872991362601 26.866700721892993 28.015997277975934 22.758572086505062 78-79 22.353657540284967 27.092354003179885 27.90730035722077 22.646688099314378 80-81 22.52501119401729 27.293135121607985 27.518676334108033 22.663177350266693 82-83 22.42998226649103 27.33633537856362 27.670558492187137 22.563123862758218 84-85 22.39620695134882 27.489210622475486 27.597149287995354 22.517433138180344 86-87 22.57073829624772 27.514035883920645 27.247458457958334 22.6677673618733 88-89 22.417937528111274 27.647195336648483 27.040867169355327 22.893999965884923 90-91 22.378435570167625 27.58549562842536 27.22397715657541 22.81209164483161 92-93 22.40348853677034 27.171094108930816 27.42379262337597 23.001624730922877 94-95 22.17871052609725 27.15773984868613 27.479992737077957 23.183556888138657 96-97 22.550118931604114 26.75316945206174 27.446155337827253 23.250556278506888 98-99 22.16586750601727 27.09004225780764 27.118725855727337 23.625364380447753 100-101 22.621460897388133 26.54209127219612 27.36264865869391 23.473799171721833 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 604.0 1 434.0 2 383.0 3 525.0 4 437.0 5 397.0 6 804.5 7 1805.0 8 2942.0 9 3427.5 10 2809.5 11 2023.5 12 1944.5 13 2085.5 14 2191.0 15 2321.0 16 2607.5 17 2953.5 18 3224.0 19 3603.0 20 4070.5 21 4781.5 22 5805.5 23 7013.5 24 8656.0 25 10893.5 26 13844.5 27 17239.5 28 20203.5 29 22949.0 30 26376.0 31 31599.0 32 39231.0 33 42588.5 34 42661.0 35 45164.5 36 49020.0 37 53451.0 38 56365.0 39 58541.5 40 61581.0 41 65468.5 42 67841.0 43 69805.5 44 72376.5 45 76186.0 46 77947.0 47 79516.0 48 80594.5 49 79069.5 50 79209.5 51 77838.5 52 76413.5 53 77685.0 54 79504.0 55 75603.0 56 69432.5 57 64572.0 58 59735.0 59 52671.5 60 42152.0 61 34776.5 62 29445.5 63 21680.5 64 15067.0 65 11413.0 66 8499.0 67 6449.0 68 4892.5 69 3617.5 70 2683.0 71 1827.0 72 1267.5 73 997.5 74 744.5 75 527.5 76 373.5 77 229.5 78 159.0 79 95.0 80 53.5 81 35.5 82 14.0 83 7.5 84 7.0 85 4.5 86 4.0 87 3.0 88 1.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015800667398644556 3 0.003052401656556334 4 0.0018404186458648487 5 0.11751746377741887 6 0.0 7 0.0 8 1.3466477896572062E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.7955303862096083E-4 24-25 5.835473755181227E-4 26-27 0.00623946809207839 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011222064913810052 36-37 4.713267263800222E-4 38-39 0.006845459597424132 40-41 0.010189634941739528 42-43 0.005409035288456445 44-45 0.0035012842531087366 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.488825965524021E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.2444129827620104E-5 68-69 8.753210632771841E-4 70-71 0.0 72-73 8.977651931048042E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.733238948286031E-5 82-83 0.0022668571125896305 84-85 0.004488825965524021 86-87 0.0031870664355220547 88-89 0.0 90-91 0.003007513396901094 92-93 2.2444129827620104E-5 94-95 2.2444129827620104E-5 96-97 1.3466477896572062E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2227754.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.50073468021747 #Duplication Level Percentage of deduplicated Percentage of total 1 79.12351544122625 45.49660268352041 2 12.497927331117108 14.372800070384065 3 3.640993123124694 6.280793386358682 4 1.5564877324855297 3.5799675255465497 5 0.8294114092321901 2.384588269150272 6 0.5016360102374351 1.730666347842337 7 0.3359782373465446 1.3523296838793555 8 0.2360762564402804 1.0859646548697237 9 0.18053690519558344 0.9342904217074935 >10 0.9802259209803446 10.314559324540978 >50 0.07169616270644749 2.7945145317151567 >100 0.039206462413136835 4.313831778640607 >500 0.0032713372193790744 1.353365605003397 >1k 0.0028040033308962533 2.9490274547306736 >5k 2.3366694424135448E-4 1.0566982621103105 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9858 0.44250846368135804 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8613 0.38662258041058395 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5123 0.2299625542137956 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3490 0.15666002619678834 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3138 0.14085935879814376 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3131 0.1405451409805571 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3092 0.13879449885400272 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2993 0.13435056114813396 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2497 0.11208598435913482 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2486 0.11159221350292717 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2437 0.10939268877982039 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2349 0.10544252193015927 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.488825965524021E-5 0.0 5 0.0 0.0 0.0 4.488825965524021E-5 0.0 6 0.0 0.0 0.0 4.488825965524021E-5 4.488825965524021E-5 7 0.0 0.0 0.0 4.488825965524021E-5 4.488825965524021E-5 8 0.0 0.0 0.0 4.488825965524021E-5 4.488825965524021E-5 9 0.0 0.0 0.0 4.488825965524021E-5 4.488825965524021E-5 10-11 0.0 0.0 0.0 4.488825965524021E-5 8.977651931048042E-5 12-13 4.488825965524021E-5 0.0 0.0 8.977651931048042E-5 3.3666194741430156E-4 14-15 4.488825965524021E-5 0.0 0.0 1.3466477896572062E-4 3.815502070695418E-4 16-17 4.488825965524021E-5 0.0 0.0 1.7955303862096083E-4 4.039943368971619E-4 18-19 4.488825965524021E-5 0.0 0.0 2.0199716844858094E-4 4.488825965524021E-4 20-21 4.488825965524021E-5 0.0 0.0 2.4688542810382113E-4 4.937708562076424E-4 22-23 6.733238948286031E-5 0.0 0.0 4.713267263800222E-4 5.386591158628825E-4 24-25 1.3466477896572062E-4 0.0 0.0 7.406562843114634E-4 5.835473755181227E-4 26-27 1.3466477896572062E-4 0.0 0.0 0.001054874101898145 5.835473755181227E-4 28-29 1.3466477896572062E-4 0.0 0.0 0.0026484073196591726 5.835473755181227E-4 30-31 1.5710890879334073E-4 0.0 0.0 0.009000096060875663 5.835473755181227E-4 32-33 1.7955303862096083E-4 0.0 0.0 0.02388055413658779 5.835473755181227E-4 34-35 1.7955303862096083E-4 0.0 0.0 0.044955592044723075 6.284356351733629E-4 36-37 1.7955303862096083E-4 0.0 0.0 0.08133752649529527 6.284356351733629E-4 38-39 1.7955303862096083E-4 0.0 0.0 0.14002892599452182 6.284356351733629E-4 40-41 1.7955303862096083E-4 0.0 0.0 0.19887743440254174 6.284356351733629E-4 42-43 1.7955303862096083E-4 0.0 0.0 0.25891548169142553 6.284356351733629E-4 44-45 2.2444129827620102E-4 0.0 0.0 0.3332953279401586 6.50879765000983E-4 46-47 2.6932955793144124E-4 0.0 0.0 0.42145586990305034 6.733238948286031E-4 48-49 2.6932955793144124E-4 0.0 0.0 0.5099979620730116 7.182121544838433E-4 50-51 2.6932955793144124E-4 0.0 0.0 0.6120963086588556 7.631004141390835E-4 52-53 2.6932955793144124E-4 0.0 0.0 0.7031970316291656 8.52876933449564E-4 54-55 2.6932955793144124E-4 0.0 0.0 0.8034549595691445 9.426534527600444E-4 56-57 2.6932955793144124E-4 0.0 0.0 0.9159898265248317 9.426534527600444E-4 58-59 2.6932955793144124E-4 0.0 0.0 1.0400609762119157 9.426534527600444E-4 60-61 2.6932955793144124E-4 0.0 0.0 1.1725262304545296 9.426534527600444E-4 62-63 2.6932955793144124E-4 0.0 0.0 1.3012657591457584 0.0010324299720705248 64-65 3.1421781758668145E-4 0.0 0.0 1.4289953019947443 0.001077318231725765 66-67 3.1421781758668145E-4 0.0 0.0 1.5815256083032507 0.0010997623615533851 68-69 3.5910607724192167E-4 0.0 0.0 1.7486894872593652 0.0011222064913810053 70-71 3.5910607724192167E-4 0.0 0.0 1.9257961157291157 0.0011446506212086254 72-73 3.5910607724192167E-4 0.0 0.0 2.1066060256204233 0.0011895388808638655 74-75 3.5910607724192167E-4 0.0 0.0 2.281490685237239 0.0012568712703467258 76-77 3.5910607724192167E-4 0.0 0.0 2.4688093927785566 0.0012568712703467258 78-79 3.5910607724192167E-4 0.0 0.0 2.68813342945406 0.0012568712703467258 80-81 3.5910607724192167E-4 0.0 0.0 2.917557324551993 0.001279315400174346 82-83 3.5910607724192167E-4 0.0 0.0 3.1582032845637356 0.0013466477896572062 84-85 3.5910607724192167E-4 0.0 0.0 3.3959539518277153 0.0013466477896572062 86-87 3.5910607724192167E-4 0.0 0.0 3.6346472725444547 0.0013466477896572062 88-89 3.5910607724192167E-4 0.0 0.0 3.898747348226061 0.0013466477896572062 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4950 0.0 55.247757 1 GTATCAA 8500 0.0 41.965687 1 TATCAAC 10940 0.0 32.605877 2 ATCAACG 11010 0.0 32.309277 3 TCAACGC 11100 0.0 32.047306 4 CAACGCA 11345 0.0 31.353827 5 AACGCAG 11640 0.0 30.520796 6 GTGGTAT 2190 0.0 29.970015 1 TGGTATC 2180 0.0 29.889322 2 ACGCAGA 13195 0.0 26.708023 7 CGCAGAG 13335 0.0 26.427626 8 GCAGAGT 14660 0.0 23.715067 9 GAGTACT 9430 0.0 23.092709 12-13 TAAGGTG 1865 0.0 22.948647 5 GTAAGGT 1790 0.0 22.317173 4 CAGAGTA 13875 0.0 21.821993 10-11 GTACTTT 10015 0.0 21.50669 14-15 GTACATG 8020 0.0 21.05261 1 CGTATAG 115 0.0036967176 20.678745 1 TACATGG 7915 0.0 20.550726 2 >>END_MODULE