FastQCFastQC Report
Thu 26 May 2016
SRR938206_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938206_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212124
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104520.4724870757697127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86880.39274471051351556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49950.22580108529178292No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34870.1576313081906801No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA30590.13828338736888168No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29430.13303955836110454No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28430.1285190161130208No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA27600.12476696604711128No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA25230.11405328091915282No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA24500.11075328507805168No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23510.10627794825244878No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG22700.10261630903150094No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA22490.10166699515940336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46700.056.710131
GTATCAA83550.043.577531
TATCAAC105100.034.500412
ATCAACG105450.034.3858953
TCAACGC106300.034.0215264
CAACGCA110300.032.8739135
GTGGTAT20450.031.9677281
AACGCAG113850.031.8848296
TGGTATC20550.030.0631122
ACGCAGA128500.027.799087
CGCAGAG128500.027.7251478
TAAGGTG15900.026.3019495
GCAGAGT140050.024.7263579
GTAAGGT16200.024.054774
GGTAAGG17200.023.4851253
GAGTACT89400.023.35217312-13
AGGTAAG15500.022.6883242
TAAGGTA7550.022.6598974
GTACTTT93100.022.42613414-15
GTGTAAG9700.022.1373331
Produced by FastQC (version 0.11.5)