##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938206_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2212124 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8349794134506 33.0 31.0 34.0 30.0 34.0 2 32.036948652064716 34.0 31.0 34.0 30.0 34.0 3 32.11246295415628 34.0 31.0 34.0 30.0 34.0 4 35.636182691386196 37.0 35.0 37.0 33.0 37.0 5 35.64425909216662 37.0 35.0 37.0 33.0 37.0 6 35.66750191218937 37.0 35.0 37.0 33.0 37.0 7 35.61086313425468 37.0 35.0 37.0 33.0 37.0 8 35.576709081407735 37.0 35.0 37.0 33.0 37.0 9 37.21046831009473 39.0 37.0 39.0 34.0 39.0 10-11 37.121579757735105 39.0 37.0 39.0 33.5 39.0 12-13 37.01953281099975 39.0 37.0 39.0 33.0 39.0 14-15 38.193018338935794 40.0 38.0 41.0 33.0 41.0 16-17 38.03303996520991 40.0 38.0 41.0 33.0 41.0 18-19 38.19309315391 40.0 38.0 41.0 33.5 41.0 20-21 38.1699988337001 40.0 38.0 41.0 33.0 41.0 22-23 38.079461187528366 40.0 38.0 41.0 33.0 41.0 24-25 37.96829314269905 40.0 38.0 41.0 33.0 41.0 26-27 37.78759554166041 40.0 38.0 41.0 32.5 41.0 28-29 37.57084661619331 40.0 37.5 41.0 32.0 41.0 30-31 37.340584659811114 40.0 37.0 41.0 31.5 41.0 32-33 37.06988916534516 40.0 36.5 41.0 31.0 41.0 34-35 36.827997661975544 39.5 36.0 41.0 30.5 41.0 36-37 36.554423034151796 39.0 35.5 41.0 30.0 41.0 38-39 36.24555336861767 39.0 35.0 40.5 30.0 41.0 40-41 36.0081123390913 39.0 35.0 40.0 29.0 41.0 42-43 35.805727436617474 39.0 35.0 40.0 27.5 41.0 44-45 35.517680970867815 39.0 35.0 40.0 27.0 41.0 46-47 35.10823534304587 38.0 34.5 40.0 25.0 41.0 48-49 34.69597206124069 38.0 34.0 40.0 24.0 41.0 50-51 33.98322901428672 37.5 33.5 39.5 23.0 40.5 52-53 34.09846283481396 38.0 33.5 40.0 23.0 40.5 54-55 34.18134901117659 38.0 34.0 40.0 22.5 41.0 56-57 33.856393448106886 38.0 33.5 40.0 21.5 41.0 58-59 33.5121157313062 37.5 33.0 40.0 19.5 41.0 60-61 33.06977886411431 37.0 32.5 40.0 17.5 41.0 62-63 32.50700367610496 36.0 32.0 39.0 12.0 41.0 64-65 31.96240875285472 36.0 31.0 39.0 8.0 40.0 66-67 31.41238194603919 35.0 31.0 38.5 4.5 40.0 68-69 30.778506765443527 35.0 30.0 38.0 2.0 40.0 70-71 30.186658162019853 35.0 29.0 37.5 2.0 39.5 72-73 29.51791513495627 34.0 28.5 37.0 2.0 39.0 74-75 28.80926250065548 34.0 27.5 36.0 2.0 39.0 76-77 28.12533361601791 34.0 26.0 35.5 2.0 38.0 78-79 27.38133734817759 33.0 25.5 35.0 2.0 37.0 80-81 26.69534506203088 32.5 24.5 35.0 2.0 36.5 82-83 25.934736705537304 32.0 21.0 35.0 2.0 36.0 84-85 25.25821427731899 31.5 19.0 35.0 2.0 36.0 86-87 24.548955212275622 31.0 15.5 34.0 2.0 35.0 88-89 23.69989069328844 31.0 7.0 34.0 2.0 35.0 90-91 22.839669928087215 30.0 2.0 34.0 2.0 35.0 92-93 21.923735513922367 29.0 2.0 34.0 2.0 35.0 94-95 20.94140563548879 29.0 2.0 34.0 2.0 35.0 96-97 19.713328231147983 27.5 2.0 34.0 2.0 35.0 98-99 18.182189832034734 24.5 2.0 33.0 2.0 35.0 100-101 14.590366317620532 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25490816978554065 1101 2 0.24250458747740566 1101 3 0.18753690124815847 1101 4 0.19634363501223362 1101 5 0.17087192246739136 1101 6 0.2199648586108367 1101 7 0.19630594640241128 1101 8 0.24843945731689132 1101 9 0.37987740231245226 1101 10-11 0.2734635654233202 1101 12-13 0.3507581718487174 1101 14-15 0.460908885936405 1101 16-17 0.5558807631779885 1101 18-19 0.47774623331277866 1101 20-21 0.5432342778284607 1101 22-23 0.48445132001184277 1101 24-25 0.5231882465441373 1101 26-27 0.48600299024266036 1101 28-29 0.49869826481235435 1101 30-31 0.6404657154561093 1101 32-33 0.33691447125359275 1101 34-35 0.5177650511369549 1101 36-37 0.5691897801659209 1101 38-39 0.6552269532992483 1101 40-41 0.8468820169620912 1101 42-43 0.917162939769085 1101 44-45 0.7145794329680868 1101 46-47 0.8555050247999816 1101 48-49 0.9209931738617456 1101 50-51 0.8211323047286001 1101 52-53 0.8200495193169601 1101 54-55 0.9099462977913291 1101 56-57 0.9887123978205565 1101 58-59 0.9583148614097681 1101 60-61 0.9972787435644292 1101 62-63 0.961928623510552 1101 64-65 0.7461019444631667 1101 66-67 0.9022724477606161 1101 68-69 1.3232034266258417 1101 70-71 1.548485718051019 1101 72-73 1.783579331425635 1101 74-75 1.9044206216710755 1101 76-77 1.934747075179807 1101 78-79 2.1002674807218824 1101 80-81 2.317966022481336 1101 82-83 2.4319917337438177 1101 84-85 2.7747903242453944 1101 86-87 2.8509441501387336 1101 88-89 3.2519539777968696 1101 90-91 3.623130280200783 1101 92-93 3.937917054435033 1101 94-95 4.1133731212672195 1101 96-97 4.3703411738507185 1101 98-99 4.700110234335433 1101 100-101 4.745211057754824 1102 1 0.4430357363523676 1102 2 0.3575207299938725 1102 3 0.37537758764589313 1102 4 0.3418831018971389 1102 5 0.4123109983970821 1102 6 0.39123309701111 1102 7 0.3037331107150365 1102 8 0.42224193345297323 1102 9 0.5117619432808596 1102 10-11 0.49212767306013205 1102 12-13 0.5503961897166647 1102 14-15 0.639123148882419 1102 16-17 0.6940095477545682 1102 18-19 0.5546172906540932 1102 20-21 0.6557878378636417 1102 22-23 0.7053296138859793 1102 24-25 0.6609737856067426 1102 26-27 0.6564606042807597 1102 28-29 0.8302796509281833 1102 30-31 0.7777363214600115 1102 32-33 0.8457270309555085 1102 34-35 0.8057468711752378 1102 36-37 0.8438993107553259 1102 38-39 0.8223299870764791 1102 40-41 1.0213604885101546 1102 42-43 1.3018862209111361 1102 44-45 1.204218186824896 1102 46-47 1.3009041095822695 1102 48-49 1.4521369057503293 1102 50-51 1.2655646266646485 1102 52-53 1.276418725542598 1102 54-55 1.2765534979393536 1102 56-57 1.3607948109518446 1102 58-59 1.465694834323294 1102 60-61 1.6441604831104044 1102 62-63 1.6023655365659053 1102 64-65 1.780683444087142 1102 66-67 1.7410779274569137 1102 68-69 1.9118192077821412 1102 70-71 2.1232782547092235 1102 72-73 2.339452159804953 1102 74-75 2.3390386356724875 1102 76-77 2.404879662020015 1102 78-79 2.6546790861261123 1102 80-81 2.670387814593507 1102 82-83 3.0439049316310154 1102 84-85 3.0324199891579546 1102 86-87 3.167679811442877 1102 88-89 3.4210006441560132 1102 90-91 3.491561644314693 1102 92-93 3.758905383828406 1102 94-95 4.058453450306079 1102 96-97 4.293637787213477 1102 98-99 4.520758168875961 1102 100-101 4.182786503092876 1103 1 0.5375584476550763 1103 2 0.5476916879184941 1103 3 0.48629341077927535 1103 4 0.3285241579121845 1103 5 0.329887865960778 1103 6 0.44310089809551556 1103 7 0.41963387131934127 1103 8 0.4270034256047879 1103 9 0.6043870014419213 1103 10-11 0.655074504350317 1103 12-13 0.7451638006499977 1103 14-15 0.8829936847238784 1103 16-17 0.9289792149783196 1103 18-19 0.9722073631536361 1103 20-21 0.8585919134113098 1103 22-23 0.9837208194580498 1103 24-25 0.8915275145619361 1103 26-27 0.9080021692644777 1103 28-29 1.0571738322102888 1103 30-31 1.0587027862642557 1103 32-33 1.2094433390809627 1103 34-35 1.1511758268398822 1103 36-37 1.206944392703086 1103 38-39 1.1913014145626803 1103 40-41 1.1870362904206857 1103 42-43 1.4656297994546819 1103 44-45 1.3877004738129628 1103 46-47 1.5014305562497583 1103 48-49 1.6544494312699811 1103 50-51 1.5453604516496924 1103 52-53 1.6274763963936891 1103 54-55 1.7519951362772304 1103 56-57 1.761791175291748 1103 58-59 1.920777577341596 1103 60-61 1.8860747596342264 1103 62-63 2.0102600703752884 1103 64-65 2.21660107733533 1103 66-67 2.3268623837340314 1103 68-69 2.455494162202857 1103 70-71 2.57854675704991 1103 72-73 2.6107576561443615 1103 74-75 2.6082314906449007 1103 76-77 2.6169568666804963 1103 78-79 2.9462973346946377 1103 80-81 3.063838967606962 1103 82-83 3.1342729843510533 1103 84-85 3.127006054478766 1103 86-87 3.277457191061341 1103 88-89 3.5267594205421453 1103 90-91 3.830127297475329 1103 92-93 4.128761690387076 1103 94-95 4.5322717717539796 1103 96-97 4.867809318440038 1103 98-99 5.02074896993188 1103 100-101 4.2577929126797205 1104 1 0.2473119037467093 1104 2 0.2395228866069985 1104 3 0.18735581291435466 1104 4 0.15730623165367064 1104 5 0.21218556132295419 1104 6 0.14747097240797302 1104 7 0.2838438580832445 1104 8 0.24844535980495408 1104 9 0.27743759706622484 1104 10-11 0.3407115563344334 1104 12-13 0.29040050940398743 1104 14-15 0.4187030269850851 1104 16-17 0.47659901570154517 1104 18-19 0.4123330898412405 1104 20-21 0.3716809412936115 1104 22-23 0.3591882461188334 1104 24-25 0.42269803310544063 1104 26-27 0.4758509468721215 1104 28-29 0.4360988374009125 1104 30-31 0.4291900508477582 1104 32-33 0.39864989246222393 1104 34-35 0.3472882103752184 1104 36-37 0.4051939893482128 1104 38-39 0.4437496596986321 1104 40-41 0.5931972117135018 1104 42-43 0.6346971305214453 1104 44-45 0.6724480634161338 1104 46-47 0.5578679324317122 1104 48-49 0.7678568986339158 1104 50-51 0.7064835570713015 1104 52-53 0.670230796530376 1104 54-55 0.8566088185503773 1104 56-57 0.9421520373249166 1104 58-59 0.7864741917559641 1104 60-61 0.8387847799209212 1104 62-63 0.9913188823275689 1104 64-65 1.0015863002727023 1104 66-67 0.8492116771601061 1104 68-69 1.0160305106409062 1104 70-71 1.287305343209976 1104 72-73 1.591665480363826 1104 74-75 1.5750004018246422 1104 76-77 1.6805382470409 1104 78-79 1.9194400368911388 1104 80-81 1.9249214671960395 1104 82-83 2.0639984002499396 1104 84-85 2.1976597975687433 1104 86-87 2.3446834990990233 1104 88-89 2.409425866607215 1104 90-91 2.628438505766489 1104 92-93 2.963085027440357 1104 94-95 3.133848262112391 1104 96-97 3.188397097328032 1104 98-99 3.1722225128799835 1104 100-101 3.0753553948369454 1105 1 0.2502758495308761 1105 2 0.2754665350553225 1105 3 0.16656174590271178 1105 4 0.05772139368935569 1105 5 0.1295023251676639 1105 6 0.19920273921930232 1105 7 0.2632345495835935 1105 8 0.2798937658363485 1105 9 0.23175024916060494 1105 10-11 0.26655916940448066 1105 12-13 0.36064645607145707 1105 14-15 0.42398765476861655 1105 16-17 0.4325876474013768 1105 18-19 0.43765127420968497 1105 20-21 0.47074654412940475 1105 22-23 0.43975600758757594 1105 24-25 0.4219270413838174 1105 26-27 0.48200470006968743 1105 28-29 0.5972468967891658 1105 30-31 0.5577393307108665 1105 32-33 0.5794270537617479 1105 34-35 0.5949306897070343 1105 36-37 0.697826913232845 1105 38-39 0.8381917167370219 1105 40-41 0.9907790331200488 1105 42-43 1.0817592954925033 1105 44-45 1.1485963355130266 1105 46-47 1.1681332098952169 1105 48-49 1.0125273143159106 1105 50-51 1.0147390565142516 1105 52-53 0.9983584200652871 1105 54-55 1.019564490277041 1105 56-57 1.0538971297668596 1105 58-59 1.1724978165946638 1105 60-61 1.2866837016011985 1105 62-63 1.2739403199460853 1105 64-65 1.419892313391589 1105 66-67 1.6343574627239121 1105 68-69 1.7716469947172477 1105 70-71 1.7536213386319126 1105 72-73 1.7888642618208088 1105 74-75 1.9870776485569976 1105 76-77 2.210541159196758 1105 78-79 2.2538954853251205 1105 80-81 2.4250206284663776 1105 82-83 2.7594918133726445 1105 84-85 2.7179700808904776 1105 86-87 2.7673068808949886 1105 88-89 3.0853606696647127 1105 90-91 3.455289569237717 1105 92-93 3.388614810656353 1105 94-95 3.6941616404988515 1105 96-97 3.9695255669706064 1105 98-99 4.27914138005341 1105 100-101 4.1456805677629 1106 1 0.0625373409496568 1106 2 0.053301766212825896 1106 3 0.016692842053323886 1106 4 0.0760391321220979 1106 5 0.12424870031619406 1106 6 -0.17001004126838382 1106 7 0.09173647720989919 1106 8 0.0492511532211779 1106 9 -0.014506755173613328 1106 10-11 0.08197862320312055 1106 12-13 0.25063395513847553 1106 14-15 0.3624372237618303 1106 16-17 0.025420844962020794 1106 18-19 0.21236885570223762 1106 20-21 0.04286080233752898 1106 22-23 -0.048683223788643204 1106 24-25 -0.07542579026509344 1106 26-27 0.002764451056847861 1106 28-29 0.06770067027880344 1106 30-31 0.15108741344213428 1106 32-33 0.13353769340479005 1106 34-35 0.18824434697631176 1106 36-37 0.26194967462986796 1106 38-39 0.05031702302384389 1106 40-41 0.4855420721878616 1106 42-43 0.28857709359605366 1106 44-45 0.27851343900055525 1106 46-47 0.5871655211349278 1106 48-49 0.6281051651532792 1106 50-51 0.6312980829102983 1106 52-53 0.5898962224156321 1106 54-55 0.2807719239416713 1106 56-57 0.6255644556728086 1106 58-59 0.8484440577932162 1106 60-61 0.830811156835118 1106 62-63 0.5861260753484885 1106 64-65 0.9364985575517331 1106 66-67 1.1512412060428687 1106 68-69 0.8303290363606841 1106 70-71 1.0661594311539808 1106 72-73 1.3300161597314535 1106 74-75 1.5490198313428287 1106 76-77 1.7246021144165127 1106 78-79 2.0785767853742563 1106 80-81 2.1658108694366334 1106 82-83 2.4292208161358637 1106 84-85 2.478873472866173 1106 86-87 2.5266000593050926 1106 88-89 2.728488070217036 1106 90-91 2.852532957852688 1106 92-93 3.178095726577201 1106 94-95 3.2028050258409095 1106 96-97 3.4664277743112706 1106 98-99 3.888828385748681 1106 100-101 3.6173251628855407 1107 1 0.22623845570995016 1107 2 0.1761874713156999 1107 3 0.05357370197506128 1107 4 0.05914339644213129 1107 5 0.14277332000326481 1107 6 0.1057380953259397 1107 7 0.0698968750538711 1107 8 0.17881424598117235 1107 9 0.1534347570620298 1107 10-11 0.12787939951495275 1107 12-13 0.23226494724864466 1107 14-15 0.3053353739685889 1107 16-17 0.3411774370079783 1107 18-19 0.1985074060722738 1107 20-21 0.3953473463809516 1107 22-23 0.40024077967186855 1107 24-25 0.330284308316557 1107 26-27 0.41876259177906405 1107 28-29 0.45094094372549876 1107 30-31 0.5298411520611594 1107 32-33 0.4593425260678714 1107 34-35 0.5107782599803912 1107 36-37 0.49608005523654697 1107 38-39 0.5554446285374723 1107 40-41 0.7035697516962856 1107 42-43 0.6541503881925124 1107 44-45 0.48651082940703105 1107 46-47 0.5001555830993283 1107 48-49 0.5655596863815688 1107 50-51 0.42990819404841574 1107 52-53 0.5532603478773339 1107 54-55 0.6234778681395312 1107 56-57 0.5274461776512567 1107 58-59 0.565984166469967 1107 60-61 0.6637629964718386 1107 62-63 0.6978986405950494 1107 64-65 0.9343868065858949 1107 66-67 1.1566382762787981 1107 68-69 1.1314827908000886 1107 70-71 1.3076109580311446 1107 72-73 1.5347630779266872 1107 74-75 1.6705115580942724 1107 76-77 1.8238261865727736 1107 78-79 1.7738210162180685 1107 80-81 2.1404930892192375 1107 82-83 2.287207723625464 1107 84-85 2.4385402581856326 1107 86-87 2.447766220449413 1107 88-89 2.751577270623912 1107 90-91 3.0223259720829603 1107 92-93 3.1246310265518176 1107 94-95 3.4226896791961927 1107 96-97 3.688304565862886 1107 98-99 3.7982420643040484 1107 100-101 3.4637087677571383 1108 1 0.28964819864981806 1108 2 0.11807337833051434 1108 3 0.09847012108604503 1108 4 0.05194300658372697 1108 5 0.09243439400595577 1108 6 0.057239006168934736 1108 7 0.06495379174034355 1108 8 0.06303378114323266 1108 9 0.11345997025208732 1108 10-11 0.11245722158815141 1108 12-13 0.18053995582904747 1108 14-15 0.21370379895824954 1108 16-17 0.23765870092363883 1108 18-19 0.2730934015312556 1108 20-21 0.2273707928876192 1108 22-23 0.1462561165957723 1108 24-25 0.09822835527557316 1108 26-27 0.2767409383570225 1108 28-29 0.3914317098896589 1108 30-31 0.2589959931696626 1108 32-33 0.2801479180823776 1108 34-35 0.4619510803375064 1108 36-37 0.5970158694069738 1108 38-39 0.43677295223435664 1108 40-41 0.3733831168871262 1108 42-43 0.5308252172444412 1108 44-45 0.5578948487327438 1108 46-47 0.7975474699963954 1108 48-49 0.7206375810581989 1108 50-51 0.8513966633041647 1108 52-53 0.6253244743041009 1108 54-55 0.6601483739874041 1108 56-57 0.7170701029005286 1108 58-59 0.8830337062082307 1108 60-61 0.9070023523694388 1108 62-63 0.9336051159406793 1108 64-65 1.24598393897055 1108 66-67 1.1714727514660268 1108 68-69 1.3153625199233012 1108 70-71 1.6304134943108544 1108 72-73 1.6611342926480255 1108 74-75 1.7411813687093343 1108 76-77 1.8813685275712686 1108 78-79 2.0662775201650554 1108 80-81 2.1717780158677797 1108 82-83 2.1378668747373943 1108 84-85 2.2515066802007517 1108 86-87 2.3088054615015885 1108 88-89 2.5019752144068583 1108 90-91 2.8448798966282 1108 92-93 3.2525623899121676 1108 94-95 3.3663832465325676 1108 96-97 3.613250773323877 1108 98-99 3.658724679083466 1108 100-101 4.009072433922183 1109 1 0.26062452450354456 1109 2 0.21820410226187192 1109 3 0.13232719625441547 1109 4 0.13565497395774173 1109 5 0.1687302108401525 1109 6 0.09849530968536158 1109 7 0.20722500370771257 1109 8 0.22883741603002505 1109 9 0.14775702240628164 1109 10-11 0.18603702664450594 1109 12-13 0.24229537939151413 1109 14-15 0.356385374075856 1109 16-17 0.29682502914646847 1109 18-19 0.25461754103780265 1109 20-21 0.28333142340849093 1109 22-23 0.3541632222240949 1109 24-25 0.15988013041349092 1109 26-27 0.2578028905010612 1109 28-29 0.2272134728436015 1109 30-31 0.12282840424354191 1109 32-33 0.13217392749111667 1109 34-35 0.06842233799479658 1109 36-37 0.07716825374379965 1109 38-39 0.18835623480111252 1109 40-41 0.3827093598221083 1109 42-43 0.40980827813049103 1109 44-45 0.4928522894204619 1109 46-47 0.6788996377291525 1109 48-49 0.6834753903112514 1109 50-51 0.6913497465275071 1109 52-53 0.7738558395865311 1109 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64-65 -1.4722794091940798 2316 66-67 -1.4672459590437583 2316 68-69 -1.7038013469582687 2316 70-71 -1.7529385673146827 2316 72-73 -1.722665640005797 2316 74-75 -1.7222433643652089 2316 76-77 -1.9071278081153977 2316 78-79 -2.623149696772888 2316 80-81 -2.6413065338586783 2316 82-83 -2.57818005571443 2316 84-85 -2.614575152977455 2316 86-87 -2.4975534558139465 2316 88-89 -2.774834856117458 2316 90-91 -2.7710302605381187 2316 92-93 -3.1026681477979103 2316 94-95 -3.074260599993803 2316 96-97 -3.3554592635273472 2316 98-99 -3.1850813639121505 2316 100-101 -3.1491232275459655 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5942.0 3 1899.0 4 1979.0 5 2342.0 6 2727.0 7 3221.0 8 4086.0 9 5410.0 10 6730.0 11 8386.0 12 10716.0 13 13011.0 14 15118.0 15 18679.0 16 22657.0 17 27635.0 18 27060.0 19 23714.0 20 23360.0 21 24369.0 22 26457.0 23 29878.0 24 33500.0 25 37409.0 26 41679.0 27 48252.0 28 55222.0 29 64043.0 30 75238.0 31 90450.0 32 108986.0 33 136602.0 34 177261.0 35 236063.0 36 304896.0 37 326139.0 38 162064.0 39 8944.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.88626543847705 18.361119904729158 14.681728809664069 23.070885847129723 2 15.668107317562901 19.47795587058834 40.25331156001373 24.60062525183503 3 19.54943635445896 23.426568682258058 29.42467629504596 27.59931866823702 4 12.862394143165165 16.515317155512697 36.74923232168808 33.87305637963406 5 13.768921793689673 37.07128773613503 34.12469540085835 15.035095069316947 6 32.62341302839108 35.834952238160426 16.937695614524813 14.603939118923678 7 28.993122374872403 32.671591251888785 19.901288192256917 18.4339981809819 8 26.83260380266448 34.35255076452613 19.353131886693387 19.461713546116002 9 26.300829131444896 16.836709542587 20.081235253440692 36.7812260725274 10-11 25.152270592316434 26.366862337072618 26.691891701201836 21.788975369409116 12-13 26.363841935666233 23.70356718864831 27.139646107750583 22.792944767934877 14-15 22.76791346923935 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29.061778430012925 22.69158082341246 40-41 22.648773922599872 25.995241875116292 29.15738505248836 22.198599149795477 42-43 22.09923392306675 26.106075147207275 29.05754563736312 22.737145292362857 44-45 22.16978839465454 26.429926993273522 29.14217378969225 22.258110822379688 46-47 22.643471718043187 26.665124737962554 28.32547899269099 22.36592455130327 48-49 22.343991987357025 26.667880018358538 28.260392809421635 22.727735184862794 50-51 22.554263420439042 27.07806508581187 28.095672354603153 22.271999139145937 52-53 22.913311973313615 26.64716338470936 28.146305811491235 22.293218830485795 54-55 22.356484267513533 26.936296462411626 28.34931079804735 22.357908472027493 56-57 22.420483763989292 27.09906631804867 28.05092366739745 22.429526250564592 58-59 22.48463874343893 26.612448738354864 27.984421840077456 22.91849067812875 60-61 22.0515336202843 26.936286034651136 28.345367490143598 22.666812854920966 62-63 22.223282218408247 26.93494014688834 28.49693997688706 22.344837657816353 64-65 21.948129573883392 26.99501117218916 28.244433573174447 22.812425680752998 66-67 21.88015814350913 26.990933195895867 28.22606203222095 22.902846628374057 68-69 22.496975374393493 26.524361573420187 28.454493174021316 22.524169878165008 70-71 22.27840506536295 26.811898575185815 27.902064766582857 23.00763159286838 72-73 22.24806879740407 26.831616043172673 27.98318269602687 22.937132463396388 74-75 22.192251829664585 27.265534488117776 27.84338636299415 22.69882731922349 76-77 22.403922575651674 26.707390070319185 28.027445913358296 22.86124144067084 78-79 22.417345727056716 26.9237733566294 27.940741973177275 22.71813894313661 80-81 22.646115658200074 27.077460768876886 27.565291010553945 22.711132562369095 82-83 22.506711734073363 27.154491504197036 27.697172164908366 22.641624596821227 84-85 22.460330413733768 27.235080587006344 27.66861241332415 22.635976585935737 86-87 22.691657753333796 27.212819648089866 27.281805680000655 22.81371691857569 88-89 22.61202587567341 27.233666953350173 27.181892093051857 22.97241507792456 90-91 22.520838392293108 27.242277935971543 27.2231725906803 23.01371108105505 92-93 22.72466990676888 26.75616245241632 27.48295871865027 23.036208922164526 94-95 22.446712586212197 26.67101985887699 27.630613854365826 23.251653700544995 96-97 22.849010797507063 26.19920426094345 27.581980611604006 23.36980432994548 98-99 22.571668282016308 26.447444842422584 27.219014507851913 23.761872367709195 100-101 23.425775712184787 25.344002042655372 27.44833999165644 23.7818822535034 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 638.0 1 443.5 2 386.0 3 517.5 4 426.5 5 403.0 6 790.5 7 1751.5 8 2963.0 9 3488.5 10 2876.0 11 2079.5 12 1919.5 13 2065.0 14 2237.5 15 2431.5 16 2618.5 17 2886.5 18 3241.5 19 3587.5 20 3984.0 21 4694.5 22 5800.0 23 7076.5 24 8686.0 25 10943.0 26 13705.0 27 16930.0 28 19967.0 29 22747.0 30 26078.5 31 31102.5 32 38351.0 33 41680.5 34 42279.0 35 45018.5 36 48519.5 37 52864.0 38 56198.0 39 58230.0 40 60861.0 41 64634.5 42 66959.5 43 68645.5 44 71150.0 45 74788.0 46 76970.0 47 78909.5 48 79719.5 49 78007.5 50 77789.0 51 76760.0 52 75578.5 53 76347.0 54 77778.5 55 74898.0 56 69557.5 57 64345.0 58 59484.0 59 52707.0 60 42374.5 61 35066.5 62 29794.0 63 22483.0 64 16277.0 65 12475.5 66 9114.5 67 6739.0 68 5172.5 69 3915.0 70 3017.0 71 2115.0 72 1464.0 73 1145.5 74 871.0 75 627.0 76 440.0 77 284.5 78 194.5 79 127.5 80 77.5 81 50.0 82 39.5 83 20.5 84 8.5 85 11.0 86 10.5 87 9.5 88 4.5 89 2.0 90 3.5 91 4.5 92 3.5 93 3.0 94 1.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4701815992231901 2 0.038650636221115996 3 0.05243829007777141 4 0.055919107608795884 5 0.08326838820970253 6 0.08055606286085229 7 0.04705884480255176 8 0.040006798895541124 9 0.04588350381804998 10-11 0.0494095267715553 12-13 0.045160217058356586 14-15 0.03801776030638427 16-17 0.03883145791103934 18-19 0.046878023112628404 20-21 0.055399245250266266 22-23 0.04705884480255176 24-25 0.06428211076775081 26-27 0.056145134721200085 28-29 0.04371364353896978 30-31 0.04547665501572245 32-33 0.04500199807967365 34-35 0.049093088814189445 36-37 0.044436930298663184 38-39 0.026377364017568634 40-41 0.024343120005930953 42-43 0.02402668204856509 44-45 0.01543765177720598 46-47 0.02748489686834915 48-49 0.027982156515638363 50-51 0.016590390050467335 52-53 0.01625134938186105 54-55 0.016409568360543983 56-57 0.015505459910927236 58-59 0.013991078257819181 60-61 0.01645477378302482 62-63 0.01699723885279487 64-65 0.017268471387679896 66-67 0.00944793329849502 68-69 0.01265751829463448 70-71 0.017946552724892456 72-73 0.016025322269456865 74-75 0.014081489102780856 76-77 0.009493138720975859 78-79 0.013810256567895833 80-81 0.017697922901247852 82-83 0.014736967728752999 84-85 0.01315477794192369 86-87 0.0037068446434286684 88-89 0.028298594473004226 90-91 0.03168900115906703 92-93 0.016386965649303566 94-95 0.032547904186202944 96-97 0.026897226376098263 98-99 0.023891065781122576 100-101 0.14621693901426863 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2212124.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.13335360628247 #Duplication Level Percentage of deduplicated Percentage of total 1 80.2768769369574 47.47040950321123 2 11.781608917111438 13.933720922929627 3 3.4491946597786685 6.118873424207793 4 1.5169173028770129 3.588016290500587 5 0.7830732521948227 2.315287376082903 6 0.4733063663055847 1.6792915633711678 7 0.3297846964723956 1.3650892549309892 8 0.21616987878190644 1.0226279904830153 9 0.16087682010060123 0.8561867301056829 >10 0.9078849696574964 9.865758653441075 >50 0.06251515420164375 2.5364257396280623 >100 0.03580262725632202 4.02143515726416 >500 0.0029942091523916955 1.2562465744678337 >1k 0.0028406599650894495 3.1016864575779706 >5k 7.67745936510662E-5 0.39442991720488013 >10k+ 7.67745936510662E-5 0.4745144445931638 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10452 0.4724870757697127 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8688 0.39274471051351556 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4995 0.22580108529178292 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3487 0.1576313081906801 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3059 0.13828338736888168 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2943 0.13303955836110454 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2843 0.1285190161130208 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2760 0.12476696604711128 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2523 0.11405328091915282 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2450 0.11075328507805168 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2351 0.10627794825244878 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2270 0.10261630903150094 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2249 0.10166699515940336 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.520542248083742E-5 2 0.0 0.0 0.0 0.0 4.520542248083742E-5 3 0.0 0.0 0.0 0.0 4.520542248083742E-5 4 0.0 0.0 0.0 0.0 4.520542248083742E-5 5 0.0 0.0 0.0 0.0 4.520542248083742E-5 6 4.520542248083742E-5 0.0 0.0 0.0 4.520542248083742E-5 7 4.520542248083742E-5 0.0 0.0 0.0 4.520542248083742E-5 8 4.520542248083742E-5 0.0 0.0 0.0 4.520542248083742E-5 9 4.520542248083742E-5 0.0 0.0 0.0 4.520542248083742E-5 10-11 4.520542248083742E-5 0.0 0.0 0.0 4.520542248083742E-5 12-13 4.520542248083742E-5 0.0 0.0 0.0 1.3561626744251225E-4 14-15 6.780813372125614E-5 0.0 0.0 2.260271124041871E-5 1.3561626744251225E-4 16-17 1.3561626744251228E-4 0.0 0.0 1.1301355620209355E-4 1.3561626744251225E-4 18-19 2.034244011637684E-4 0.0 0.0 2.260271124041871E-4 1.3561626744251225E-4 20-21 2.260271124041871E-4 0.0 0.0 3.3904066860628067E-4 1.5821897868293098E-4 22-23 2.260271124041871E-4 0.0 0.0 5.42465069770049E-4 1.808216899233497E-4 24-25 2.260271124041871E-4 0.0 0.0 7.232867596933988E-4 1.808216899233497E-4 26-27 2.260271124041871E-4 0.0 0.0 0.0011527382732613542 1.808216899233497E-4 28-29 2.260271124041871E-4 0.0 0.0 0.0032321877073798752 1.808216899233497E-4 30-31 2.260271124041871E-4 0.0 0.0 0.009741768544620465 1.808216899233497E-4 32-33 2.712325348850245E-4 0.0 0.0 0.02474996880825849 1.808216899233497E-4 34-35 2.9383524612544326E-4 0.0 0.0 0.0480759668083706 1.808216899233497E-4 36-37 3.1643795736586197E-4 0.0 0.0 0.08433071563800221 1.808216899233497E-4 38-39 3.1643795736586197E-4 0.0 0.0 0.13814777110143917 2.486298236446058E-4 40-41 4.068488023275368E-4 0.0 0.0 0.19838399655715502 2.712325348850245E-4 42-43 4.068488023275368E-4 0.0 0.0 0.2587106328578326 2.9383524612544326E-4 44-45 4.068488023275368E-4 0.0 0.0 0.33341259350741637 3.1643795736586197E-4 46-47 4.068488023275368E-4 0.0 0.0 0.42027481280434553 3.1643795736586197E-4 48-49 4.068488023275368E-4 0.0 0.0 0.5102110008299715 3.1643795736586197E-4 50-51 4.068488023275368E-4 0.0 0.0 0.6098211492664968 3.616433798466994E-4 52-53 4.068488023275368E-4 0.0 0.0 0.7006388430304993 4.294515135679555E-4 54-55 4.068488023275368E-4 0.0 0.0 0.8005428267131499 4.520542248083742E-4 56-57 4.068488023275368E-4 0.0 0.0 0.9151159699908323 4.746569360487929E-4 58-59 4.068488023275368E-4 0.0 0.0 1.0358370507259087 4.972596472892116E-4 60-61 4.068488023275368E-4 0.0 0.0 1.1679725006373967 5.42465069770049E-4 62-63 4.068488023275368E-4 0.0 0.0 1.2974182279112743 5.42465069770049E-4 64-65 4.068488023275368E-4 0.0 0.0 1.4255077925107273 5.42465069770049E-4 66-67 4.068488023275368E-4 0.0 0.0 1.5801329401064317 5.42465069770049E-4 68-69 4.520542248083742E-4 0.0 0.0 1.7482293036014256 5.42465069770049E-4 70-71 4.520542248083742E-4 0.0 0.0 1.9225414126875346 5.650677810104677E-4 72-73 4.520542248083742E-4 0.0 0.0 2.103882964969414 6.780813372125613E-4 74-75 4.972596472892116E-4 0.0 0.0 2.282986848838492 8.136976046550736E-4 76-77 4.972596472892116E-4 0.0 0.0 2.4762174272328314 8.136976046550736E-4 78-79 4.972596472892116E-4 2.260271124041871E-5 0.0 2.6922089358462724 8.136976046550736E-4 80-81 4.972596472892116E-4 4.520542248083742E-5 0.0 2.9135572870236928 8.136976046550736E-4 82-83 4.972596472892116E-4 4.520542248083742E-5 0.0 3.1437659010073578 8.58903027135911E-4 84-85 4.972596472892116E-4 4.520542248083742E-5 0.0 3.3818176557914477 8.58903027135911E-4 86-87 4.972596472892116E-4 4.520542248083742E-5 0.0 3.628526248980618 8.815057383763297E-4 88-89 4.972596472892116E-4 4.520542248083742E-5 0.0 3.8839142832861087 9.267111608571672E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4670 0.0 56.71013 1 GTATCAA 8355 0.0 43.57753 1 TATCAAC 10510 0.0 34.50041 2 ATCAACG 10545 0.0 34.385895 3 TCAACGC 10630 0.0 34.021526 4 CAACGCA 11030 0.0 32.873913 5 GTGGTAT 2045 0.0 31.967728 1 AACGCAG 11385 0.0 31.884829 6 TGGTATC 2055 0.0 30.063112 2 ACGCAGA 12850 0.0 27.79908 7 CGCAGAG 12850 0.0 27.725147 8 TAAGGTG 1590 0.0 26.301949 5 GCAGAGT 14005 0.0 24.726357 9 GTAAGGT 1620 0.0 24.05477 4 GGTAAGG 1720 0.0 23.485125 3 GAGTACT 8940 0.0 23.352173 12-13 AGGTAAG 1550 0.0 22.688324 2 TAAGGTA 755 0.0 22.659897 4 GTACTTT 9310 0.0 22.426134 14-15 GTGTAAG 970 0.0 22.137333 1 >>END_MODULE