FastQCFastQC Report
Thu 26 May 2016
SRR938206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938206_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212124
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97990.4429679348897259No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87300.3946433382577107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51420.23244628239646603No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34050.15392446354725142No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA32230.14569707665573903No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC31590.14280392961696542No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30010.1356614728649931No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA28400.12838339984557828No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25210.11396287007419115No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA24380.11021082000828163No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA23610.10673000247725715No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG22710.10266151445398179No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA22610.10220946022917342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46450.056.5497131
GTATCAA80650.040.1671521
TGGTATC19800.033.1058652
ATCAACG102100.031.494473
GTGGTAT21450.031.4450361
TATCAAC103300.031.3139482
TCAACGC104000.031.1924114
CAACGCA105900.030.6327745
AACGCAG109650.029.585146
ACGCAGA126400.025.5143367
CGCAGAG125700.025.4674938
TAAGGTG15700.022.9919345
GCAGAGT139400.022.8283029
AGGACCG8850.022.540715
GAGTACT91850.022.10642612-13
CGTCGAT652.9387404E-421.92815238-39
GGACCGA7850.021.7818326
TAAGGTA9000.021.6372954
GTGTAAG10850.021.4514851
GTAAGGT15350.021.3502164
Produced by FastQC (version 0.11.5)