FastQCFastQC Report
Thu 26 May 2016
SRR938205_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938205_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231435
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105980.47494101329413585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86490.387598115114265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51810.23218242969210395No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA35630.1596730355130219No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31080.13928256928837274No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30650.13735555819461467No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28700.12861678695547932No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA28140.12610719111244556No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA25250.11315588399393216No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA24470.109660375498278No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23740.1063889380600376No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG23110.10356564273662464No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA22420.10047346214431521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45950.057.035411
GTATCAA82900.043.5336461
TATCAAC103800.034.792182
ATCAACG104150.034.538383
TCAACGC104750.034.431284
CAACGCA107450.033.7429855
TGGTATC18600.032.956582
GTGGTAT18700.032.931071
AACGCAG112100.032.4280786
ACGCAGA126750.028.4354117
CGCAGAG127700.027.9998368
GCAGAGT138550.025.2569189
TAAGGTG14950.024.1567125
GAGTACT90500.023.74299612-13
TAAGGTA8750.022.809024
GTGTAAG11250.022.4895651
CAGAGTA132950.022.40433510-11
GTACTTT95700.022.13968314-15
TACATGG76050.021.8067822
GTACATG77600.021.4694771
Produced by FastQC (version 0.11.5)