##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938205_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2231435 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87224006076807 33.0 31.0 34.0 30.0 34.0 2 32.06402382323483 34.0 31.0 34.0 30.0 34.0 3 32.13352125426015 34.0 31.0 34.0 30.0 34.0 4 35.65401860237919 37.0 35.0 37.0 33.0 37.0 5 35.6770495219444 37.0 35.0 37.0 35.0 37.0 6 35.70320309576573 37.0 35.0 37.0 35.0 37.0 7 35.64768008030707 37.0 35.0 37.0 33.0 37.0 8 35.620817097517964 37.0 35.0 37.0 33.0 37.0 9 37.282402131363895 39.0 37.0 39.0 34.0 39.0 10-11 37.20395575044758 39.0 37.0 39.0 34.0 39.0 12-13 37.10944638763845 39.0 37.0 39.0 33.5 39.0 14-15 38.353491139110034 40.0 38.0 41.0 33.5 41.0 16-17 38.27550746492727 40.0 38.0 41.0 33.0 41.0 18-19 38.217345116483344 40.0 38.0 41.0 33.5 41.0 20-21 38.10599367671476 40.0 38.0 41.0 33.0 41.0 22-23 37.95581699668599 40.0 38.0 41.0 33.0 41.0 24-25 37.90812436839971 40.0 38.0 41.0 33.0 41.0 26-27 37.91804018490343 40.0 38.0 41.0 33.0 41.0 28-29 37.8272391980945 40.0 38.0 41.0 33.0 41.0 30-31 37.63048061001105 40.0 37.5 41.0 32.5 41.0 32-33 37.39316224761196 40.0 37.0 41.0 32.0 41.0 34-35 37.18468384694154 40.0 37.0 41.0 31.0 41.0 36-37 36.955220071389036 40.0 36.0 41.0 30.5 41.0 38-39 36.693196530483746 39.5 36.0 41.0 30.0 41.0 40-41 36.45576120299269 39.0 35.5 41.0 30.0 41.0 42-43 36.167220869082 39.0 35.0 41.0 29.5 41.0 44-45 35.92531330735603 39.0 35.0 40.5 28.5 41.0 46-47 35.75616116983018 39.0 35.0 41.0 27.5 41.0 48-49 35.4795203534945 39.0 35.0 41.0 26.5 41.0 50-51 34.82184244667669 38.5 34.5 40.0 25.0 40.5 52-53 34.88943751442457 38.5 34.5 40.0 26.0 41.0 54-55 35.01569909049557 39.0 35.0 40.0 25.5 41.0 56-57 34.788757458765325 38.5 34.5 40.0 24.5 41.0 58-59 34.508063869214205 38.0 34.0 40.0 24.0 41.0 60-61 34.12097551575556 38.0 34.0 40.0 23.0 41.0 62-63 33.6926874410413 37.0 33.5 40.0 22.0 41.0 64-65 33.25248774891493 36.5 33.0 39.5 20.5 41.0 66-67 32.812845545579414 36.0 32.5 39.0 18.5 41.0 68-69 32.31643942126927 35.5 32.0 39.0 15.5 40.5 70-71 31.815429084871397 35.0 31.5 38.0 11.5 40.0 72-73 31.32968762253886 35.0 31.0 37.5 7.5 39.5 74-75 30.803083889963183 35.0 30.5 37.0 4.5 39.0 76-77 30.252305579145258 34.5 30.0 36.0 2.0 39.0 78-79 29.68994906864865 34.0 29.0 36.0 2.0 38.0 80-81 29.160343232045747 34.0 29.0 35.0 2.0 37.0 82-83 28.617230168030886 34.0 28.5 35.0 2.0 37.0 84-85 28.12674624176819 34.0 27.0 35.0 2.0 36.0 86-87 27.62642940529301 33.5 26.5 35.0 2.0 36.0 88-89 27.06046848776684 33.0 25.5 35.0 2.0 35.0 90-91 26.442399397696995 33.0 24.5 35.0 2.0 35.0 92-93 25.825337058888117 32.0 23.5 35.0 2.0 35.0 94-95 25.2065343601763 32.0 19.5 34.0 2.0 35.0 96-97 24.35195289130089 31.5 9.5 34.0 2.0 35.0 98-99 23.248750243677275 31.0 2.0 34.0 2.0 35.0 100-101 20.32449768870704 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5651070826030491 1101 2 0.40975412347838613 1101 3 0.43122245491211686 1101 4 0.3303740690607242 1101 5 0.29275585600567666 1101 6 0.3237344585955242 1101 7 0.42254520532641493 1101 8 0.3820048903190525 1101 9 0.27919943463147234 1101 10-11 0.3712150231528071 1101 12-13 0.286855518357676 1101 14-15 0.39645391668781116 1101 16-17 0.6457828732275317 1101 18-19 0.6183588855578819 1101 20-21 0.6974704535627794 1101 22-23 0.6248544965630529 1101 24-25 0.6473238290524819 1101 26-27 0.666678204275037 1101 28-29 0.5930867632644308 1101 30-31 0.6362867224695066 1101 32-33 0.8069795439643386 1101 34-35 0.7640822864317514 1101 36-37 0.8293564279139858 1101 38-39 0.8884463898883439 1101 40-41 0.8142464375308194 1101 42-43 0.902267612449215 1101 44-45 0.9732529015316729 1101 46-47 0.9667212584935427 1101 48-49 1.155439839361378 1101 50-51 1.149092781260876 1101 52-53 0.9811436279278567 1101 54-55 0.9815058357890578 1101 56-57 0.9658171613837538 1101 58-59 0.9211760883574343 1101 60-61 0.9927254821650067 1101 62-63 1.076691003142578 1101 64-65 1.0342512084761495 1101 66-67 0.9877487529817728 1101 68-69 0.966871045983396 1101 70-71 1.0516066442695653 1101 72-73 1.042331709893329 1101 74-75 1.0084918672933831 1101 76-77 1.0440383290296538 1101 78-79 1.290259131658047 1101 80-81 1.172825842859318 1101 82-83 1.1428648057316764 1101 84-85 1.2556380177123643 1101 86-87 1.327826850231478 1101 88-89 1.2704671621519985 1101 90-91 1.3476887285424688 1101 92-93 1.4900466289058159 1101 94-95 1.6224833349877876 1101 96-97 1.7572177610872863 1101 98-99 1.79157618632253 1101 100-101 1.771313963060006 1102 1 0.3035575631147722 1102 2 0.26942941124160313 1102 3 0.14231901283517345 1102 4 0.009743565441752366 1102 5 0.054768905649318356 1102 6 0.14479437531913675 1102 7 0.05213279431291795 1102 8 0.15983003863843948 1102 9 0.23438861239515774 1102 10-11 0.261927207608565 1102 12-13 0.13415760355077566 1102 14-15 0.12493208587965654 1102 16-17 0.07372381430404573 1102 18-19 0.13319852002831567 1102 20-21 0.215202085250489 1102 22-23 0.2263288661928229 1102 24-25 0.15201428222952273 1102 26-27 0.1557921716416928 1102 28-29 0.21219361991946073 1102 30-31 0.08956047152808821 1102 32-33 0.26897302119467525 1102 34-35 0.2226400740461898 1102 36-37 0.2590844098468281 1102 38-39 0.22600741580087913 1102 40-41 0.4842036039584059 1102 42-43 0.3728117717127404 1102 44-45 0.3403745562650684 1102 46-47 0.32453793654966034 1102 48-49 0.3176728285485808 1102 50-51 0.4126327613139509 1102 52-53 0.43837200490023776 1102 54-55 0.2692626371385529 1102 56-57 0.24402888410499202 1102 58-59 0.1662506636129777 1102 60-61 0.18247541187875527 1102 62-63 0.27354972058773086 1102 64-65 0.21568196471520906 1102 66-67 0.2909026049763668 1102 68-69 0.1811837131056393 1102 70-71 0.10916441751892236 1102 72-73 0.24398784901291393 1102 74-75 0.21233714108143076 1102 76-77 0.12737512537189488 1102 78-79 0.0644927549606571 1102 80-81 0.16704608099120222 1102 82-83 0.21991380397297888 1102 84-85 0.3370183422809525 1102 86-87 0.28897843151830216 1102 88-89 0.4071720040027955 1102 90-91 0.44542138117650865 1102 92-93 0.4262392789131013 1102 94-95 0.41195907135174537 1102 96-97 0.19286030571648993 1102 98-99 0.4889953981724844 1102 100-101 0.41042162005487626 1103 1 0.4601085829188065 1103 2 0.39498273016841523 1103 3 0.3453865656015225 1103 4 0.24471194016916087 1103 5 0.26281608206998897 1103 6 0.2558089495867222 1103 7 0.27788814988755206 1103 8 0.27376143991924096 1103 9 0.3272790932374434 1103 10-11 0.325203038203675 1103 12-13 0.3550606259598226 1103 14-15 0.37267749010096196 1103 16-17 0.3388365995975562 1103 18-19 0.3398151585726623 1103 20-21 0.35028294924700987 1103 22-23 0.4697640319171441 1103 24-25 0.5161028231252942 1103 26-27 0.3829796685581002 1103 28-29 0.4789884404793341 1103 30-31 0.5676993168356006 1103 32-33 0.5626088667883451 1103 34-35 0.5498299144890382 1103 36-37 0.6371755212210672 1103 38-39 0.6330638972549565 1103 40-41 0.6147906943346513 1103 42-43 0.5446230956324953 1103 44-45 0.37203695524674885 1103 46-47 0.44132461643875587 1103 48-49 0.33198758141755036 1103 50-51 0.35140640626229214 1103 52-53 0.45374196138465095 1103 54-55 0.4105054955500904 1103 56-57 0.4146753520406179 1103 58-59 0.5440431603813707 1103 60-61 0.5195501235016593 1103 62-63 0.4374157498152087 1103 64-65 0.4421414883132897 1103 66-67 0.5181570774115585 1103 68-69 0.5416487920948185 1103 70-71 0.6637947323901976 1103 72-73 0.6687623771006947 1103 74-75 0.496967307940519 1103 76-77 0.5963940758667512 1103 78-79 0.5423661650629832 1103 80-81 0.6397330203950098 1103 82-83 0.6292118067950767 1103 84-85 0.7232483976375121 1103 86-87 0.6663580976027887 1103 88-89 0.6494162882651509 1103 90-91 0.9017892358877369 1103 92-93 0.9505993728515101 1103 94-95 1.0615648655358498 1103 96-97 1.0484834123938143 1103 98-99 1.075706863197201 1103 100-101 0.9763120860959447 1104 1 0.32655377330765845 1104 2 0.23199233900901106 1104 3 0.13248672594112065 1104 4 0.07333313498754279 1104 5 0.18928019022423825 1104 6 0.16027884842281992 1104 7 0.15064656771639306 1104 8 0.19335286232756488 1104 9 0.03546333381605393 1104 10-11 0.31941985425147124 1104 12-13 0.11407021172997389 1104 14-15 0.1768192118483185 1104 16-17 0.2669940784592626 1104 18-19 0.2665007430047268 1104 20-21 0.07201242520184081 1104 22-23 0.11988053766745566 1104 24-25 0.2540831209647436 1104 26-27 0.206027620479297 1104 28-29 0.24906434224538287 1104 30-31 0.23497735031082811 1104 32-33 0.23229603154593548 1104 34-35 0.3308475518447054 1104 36-37 0.32923755764930007 1104 38-39 0.34331467299868734 1104 40-41 0.2768749937429007 1104 42-43 0.39844529170822085 1104 44-45 0.5286183766168975 1104 46-47 0.5732518061124168 1104 48-49 0.5503839547435021 1104 50-51 0.5880209337218858 1104 52-53 0.4620519358918074 1104 54-55 0.7327546332921884 1104 56-57 0.5574162363307948 1104 58-59 0.6065767567177858 1104 60-61 0.6543466998475296 1104 62-63 0.6841512817620057 1104 64-65 0.628261154714643 1104 66-67 0.7084686437794687 1104 68-69 0.7282402491088078 1104 70-71 0.7563729700388642 1104 72-73 0.7981523945188833 1104 74-75 0.61345287160918 1104 76-77 0.6390040353781181 1104 78-79 0.7625568422959184 1104 80-81 0.9020572524375474 1104 82-83 0.8701393540606546 1104 84-85 0.8760399215557975 1104 86-87 0.8504319922400612 1104 88-89 0.7936821290098663 1104 90-91 0.7480339441042752 1104 92-93 0.7620515420501555 1104 94-95 0.8154943391836333 1104 96-97 0.8196442018644632 1104 98-99 1.0681599436784524 1104 100-101 1.0764879982436852 1105 1 0.32929054497412125 1105 2 0.24489322746883602 1105 3 0.25915927447182696 1105 4 0.23828915952940832 1105 5 0.2181015371284829 1105 6 0.23695192973106316 1105 7 0.19598487079861826 1105 8 0.3488580526463565 1105 9 0.3425580126470962 1105 10-11 0.4175151745466721 1105 12-13 0.2972327365840428 1105 14-15 0.4539354575141772 1105 16-17 0.4060027712576044 1105 18-19 0.48606390804428656 1105 20-21 0.5199471663465474 1105 22-23 0.456832815717668 1105 24-25 0.4350285453646805 1105 26-27 0.2783829660494632 1105 28-29 0.34495332419275115 1105 30-31 0.3894174117720368 1105 32-33 0.37293619572665904 1105 34-35 0.5006426882250992 1105 36-37 0.3499188096338983 1105 38-39 0.4371618383025009 1105 40-41 0.5348864761644379 1105 42-43 0.6288442994633243 1105 44-45 0.5303096256392479 1105 46-47 0.6170243825606647 1105 48-49 0.4202975808487466 1105 50-51 0.3841232397961889 1105 52-53 0.41358686798398736 1105 54-55 0.373212480998788 1105 56-57 0.5859150397124466 1105 58-59 0.5320882764337469 1105 60-61 0.5788358194646079 1105 62-63 0.5472580354632086 1105 64-65 0.6742988881511209 1105 66-67 0.7927041825986976 1105 68-69 0.7816893443459847 1105 70-71 0.8598895149125845 1105 72-73 0.8709480274479411 1105 74-75 0.8725565479873119 1105 76-77 0.8430292762468419 1105 78-79 0.8673585531511527 1105 80-81 0.906140566058113 1105 82-83 0.923857928177469 1105 84-85 0.9602740751534284 1105 86-87 0.8520020876498648 1105 88-89 0.9832581233325861 1105 90-91 0.8703283399238906 1105 92-93 0.9577381750879077 1105 94-95 0.9962836597644014 1105 96-97 1.049258321747935 1105 98-99 0.8157021343318291 1105 100-101 1.0089276505317528 1106 1 0.11943362340976904 1106 2 0.1650414558325224 1106 3 0.17298652593690633 1106 4 0.23143751613240937 1106 5 0.12201772154068635 1106 6 0.25140287208647294 1106 7 0.2232986235491481 1106 8 0.1607205963851257 1106 9 0.15630587786961314 1106 10-11 -0.41442191083832114 1106 12-13 0.04068550889412137 1106 14-15 -0.008746800067719107 1106 16-17 0.17191523469412573 1106 18-19 0.313010143202618 1106 20-21 0.1947381667964052 1106 22-23 0.0911630909843737 1106 24-25 0.138973329179656 1106 26-27 0.2968832174392304 1106 28-29 0.10265389149905246 1106 30-31 0.22327605133610717 1106 32-33 0.19261624881367112 1106 34-35 -0.12067669077092802 1106 36-37 -0.16058012272291933 1106 38-39 -0.22510150418399633 1106 40-41 0.1382941815152705 1106 42-43 -0.08476067052257008 1106 44-45 0.06566968296018416 1106 46-47 0.13000036924006508 1106 48-49 0.06248526213944672 1106 50-51 0.16163932568803574 1106 52-53 0.27792911225341754 1106 54-55 -0.10860623746299325 1106 56-57 -0.12577814670883924 1106 58-59 -0.030002960749946794 1106 60-61 -0.042001644328138354 1106 62-63 0.09597889918553193 1106 64-65 0.12918327939095064 1106 66-67 -0.19629461439438955 1106 68-69 -0.3741134846540106 1106 70-71 -0.07246974243929216 1106 72-73 0.08257943508816012 1106 74-75 0.17515838119885174 1106 76-77 0.3624929509342323 1106 78-79 0.5380763269166451 1106 80-81 0.3144385657283557 1106 82-83 0.2788542743747655 1106 84-85 0.2271873141324825 1106 86-87 0.42096084547907964 1106 88-89 0.21825422526452698 1106 90-91 0.23326310682349316 1106 92-93 0.4545250678085928 1106 94-95 0.43475158670463543 1106 96-97 0.7630651159889688 1106 98-99 0.5849487181789641 1106 100-101 0.24848846141133407 1107 1 0.25840023982073745 1107 2 0.19815467050047886 1107 3 0.17321103149244976 1107 4 0.11074836149259681 1107 5 0.21747129050163494 1107 6 0.2236479857037068 1107 7 0.07190523756023026 1107 8 0.12309830375761521 1107 9 0.1762986782086884 1107 10-11 0.30499665848067536 1107 12-13 0.22559738078660274 1107 14-15 0.32874030537828247 1107 16-17 0.3139499324148147 1107 18-19 0.4376979159720378 1107 20-21 0.38084436953652556 1107 22-23 0.40334638815811985 1107 24-25 0.4906695073596481 1107 26-27 0.519917100359585 1107 28-29 0.5048126285391774 1107 30-31 0.6339952239170117 1107 32-33 0.633075633629737 1107 34-35 0.7095284995652662 1107 36-37 0.6161069107264794 1107 38-39 0.6460672407718917 1107 40-41 0.6584067826637323 1107 42-43 0.7682781863570725 1107 44-45 0.6511033367300456 1107 46-47 0.6773075605787895 1107 48-49 0.6658969724266441 1107 50-51 0.5845362795734346 1107 52-53 0.6977120192519877 1107 54-55 0.6910891317228476 1107 56-57 0.6714240495748172 1107 58-59 0.6503872531302974 1107 60-61 0.5547018012515181 1107 62-63 0.653746597868107 1107 64-65 0.6581814620571933 1107 66-67 0.6993239467959498 1107 68-69 0.8744226810620574 1107 70-71 0.8719202314522612 1107 72-73 0.9357178679232625 1107 74-75 0.9015681696410773 1107 76-77 1.171727870838005 1107 78-79 1.0840000027693328 1107 80-81 1.11746135884842 1107 82-83 1.0888648996808001 1107 84-85 1.0899306171898218 1107 86-87 1.1891749231386441 1107 88-89 1.2073778987947357 1107 90-91 1.3044189814177862 1107 92-93 1.3510734884313642 1107 94-95 1.2559236218281384 1107 96-97 1.2808071513640797 1107 98-99 1.2896126275561386 1107 100-101 0.8802834800975496 1108 1 0.26676846298022383 1108 2 0.1059185904648885 1108 3 0.17640805442351848 1108 4 0.06710857535352233 1108 5 0.09895203201668323 1108 6 0.1054477304275494 1108 7 0.12893745541256152 1108 8 0.17554250487165746 1108 9 0.1836280252896998 1108 10-11 0.16423272122312937 1108 12-13 0.048324501320884394 1108 14-15 0.053888961977399674 1108 16-17 0.1872431088736306 1108 18-19 0.1508342804613534 1108 20-21 0.157061594900334 1108 22-23 0.17283612284089855 1108 24-25 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-0.6032836217323023 2316 58-59 -0.5834801020256108 2316 60-61 -0.6724235219603614 2316 62-63 -0.639015926616473 2316 64-65 -0.657508202034073 2316 66-67 -0.8278504034796441 2316 68-69 -0.7735503393833838 2316 70-71 -0.9530091425532099 2316 72-73 -0.9561729013229545 2316 74-75 -0.795013370086675 2316 76-77 -0.7756725752696703 2316 78-79 -0.9996556095169282 2316 80-81 -0.8604441123402644 2316 82-83 -0.882807726154688 2316 84-85 -0.9599116855225738 2316 86-87 -0.9132057075424136 2316 88-89 -1.0036105717384523 2316 90-91 -0.9200319347706447 2316 92-93 -1.1067727333578041 2316 94-95 -1.1439956958386652 2316 96-97 -1.038428983820907 2316 98-99 -1.048657898376863 2316 100-101 -1.0193954119371362 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5886.0 3 1688.0 4 1784.0 5 1934.0 6 2271.0 7 2773.0 8 3477.0 9 4302.0 10 5387.0 11 6959.0 12 8608.0 13 10837.0 14 12630.0 15 15519.0 16 18749.0 17 24675.0 18 22222.0 19 18433.0 20 17632.0 21 17835.0 22 18684.0 23 20561.0 24 22959.0 25 25287.0 26 28859.0 27 33306.0 28 38969.0 29 45703.0 30 54520.0 31 67150.0 32 83187.0 33 108502.0 34 148093.0 35 217752.0 36 326198.0 37 438368.0 38 315126.0 39 34607.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.89428093662053 18.33127594349076 14.70845337276076 23.06598974712795 2 15.657883822543656 19.469128523356776 40.16997751418535 24.703010139914227 3 19.56957666145023 23.393927708250192 29.435189876823326 27.601305753476257 4 12.87484360495621 16.450516383922363 36.70056907615934 33.974070934962086 5 13.780975148120076 37.003511824937995 34.12493664811335 15.090576378828585 6 32.60970704261496 35.80222875342256 16.973035394549655 14.615028809412825 7 28.99144834588344 32.66692408181496 19.940301211600183 18.401326360701407 8 26.777835767091005 34.32947913640162 19.3567131896394 19.535971906867967 9 26.27880637863053 16.75897022941082 20.105482526738292 36.85674086522036 10-11 25.077330233396705 26.395363908431673 26.73740191046152 21.789903947710094 12-13 26.337659307911366 23.69456398729911 27.14845708402171 22.81931962076782 14-15 22.78008551791428 25.256235382319602 25.756621898899457 26.207057200866657 16-17 22.156287008682764 28.34910502046814 26.83981218366755 22.65479578718155 18-19 22.14740437911952 26.57246389484158 29.34949057949861 21.930641146540285 20-21 22.519243680632268 25.726658333680913 29.908355096419104 21.845742889267715 22-23 22.36462314442253 25.772798055546524 29.696190355537667 22.166388444493276 24-25 22.217529820610956 25.83747220350121 29.80616743052137 22.138830545366464 26-27 21.558970734543518 26.30590459627563 29.651261737451073 22.48386293172978 28-29 21.825153288255915 26.741745866066037 29.403451101915245 22.029649743762803 30-31 21.897050266226113 26.17326168193912 29.38257292487703 22.547115126957735 32-33 21.508795100889433 26.22622957068875 29.44193237711581 22.823042951306007 34-35 22.414905807192333 26.424933904701682 29.162008041314323 21.99815224679166 36-37 22.505286765696777 25.83103292784459 29.14767538148687 22.516004924971764 38-39 21.921735387870935 26.34271982640741 29.033853244325286 22.701691541396364 40-41 22.60502351789723 25.98078665584065 29.165084385111562 22.24910544115056 42-43 22.112511592321507 26.108026425897577 28.997537955676027 22.78192402610489 44-45 22.14259828724994 26.445988712999057 29.180265924270277 22.231147075480724 46-47 22.65406259777821 26.68321966779839 28.29462698369295 22.368090750730452 48-49 22.34725535013634 26.74729730759524 28.221927165258148 22.683520177010273 50-51 22.55058740628361 27.074502216963676 28.104169388889922 22.270740987862787 52-53 22.88656632922014 26.637745240426163 28.15629942013706 22.319389010216632 54-55 22.349132608030096 26.951906487251907 28.341020125214992 22.357940779503004 56-57 22.45552592846271 27.090560960626046 28.04215917630655 22.411753934604686 58-59 22.426820196951045 26.650337377107302 27.966160763491775 22.956681662449874 60-61 22.04318548177422 26.91124603056487 28.39896610895842 22.64660237870249 62-63 22.188834045421864 26.974476870420204 28.51643391561437 22.320255168543557 64-65 21.889230198242814 27.019732840389594 28.30158528806773 22.78945167329986 66-67 21.867973590504917 27.00196294056071 28.249782673637547 22.88028079529683 68-69 22.5183548332153 26.520941094208027 28.461667757348657 22.499036315228015 70-71 22.257140007016435 26.856738075143106 27.952781411155236 22.933340506685227 72-73 22.218742805958186 26.89782045330393 28.005694486878628 22.877742253859257 74-75 22.155282476859238 27.32824194780463 27.865271646158867 22.65120392917726 76-77 22.331380027692624 26.81939595296826 28.06096949284129 22.788254526497827 78-79 22.369224287462295 27.01641783686147 27.989610552594897 22.624747323081337 80-81 22.577442570369687 27.1960167750122 27.577069428766148 22.649471225851965 82-83 22.43246798856367 27.255480318903004 27.768241526463598 22.54381016606973 84-85 22.414422547029194 27.371091751382597 27.7109565091889 22.50352919239931 86-87 22.61893877463213 27.421666594957404 27.33465188738777 22.62474274302269 88-89 22.513464105619114 27.40091483063528 27.2747305217404 22.810890542005207 90-91 22.415666278214182 27.442531376991564 27.324829825976575 22.81697251881768 92-93 22.57939788332084 26.973560338984264 27.626706666714483 22.82033511098041 94-95 22.344501619597306 26.840498362466953 27.78156421174217 23.03343580619357 96-97 22.729287219922092 26.44713784817581 27.680039956447832 23.14353497545427 98-99 22.423543620225665 26.711731238997043 27.370014403866854 23.494710736910434 100-101 22.8274975725791 26.09496546396172 27.732301823472294 23.345235139986883 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 676.0 1 464.5 2 413.5 3 560.5 4 435.0 5 404.5 6 846.5 7 1847.5 8 3062.5 9 3621.5 10 2999.5 11 2212.0 12 2073.0 13 2167.0 14 2324.0 15 2555.0 16 2766.0 17 2933.5 18 3247.0 19 3639.5 20 4067.5 21 4793.5 22 5833.5 23 7110.5 24 8699.0 25 10910.0 26 13916.0 27 17309.0 28 20064.5 29 22895.0 30 26059.5 31 30939.0 32 38755.0 33 42049.0 34 42439.0 35 45199.5 36 49041.5 37 53467.0 38 56613.5 39 58948.5 40 61575.0 41 65393.5 42 67935.5 43 70046.0 44 72726.0 45 76478.0 46 78463.0 47 80031.0 48 81014.5 49 79071.0 50 78740.0 51 77854.0 52 76398.0 53 76995.5 54 78995.0 55 75815.5 56 69936.5 57 64693.0 58 59906.0 59 52929.5 60 41910.0 61 34551.5 62 29460.0 63 21737.5 64 15254.5 65 11875.0 66 8738.5 67 6402.5 68 4993.5 69 3779.0 70 2846.5 71 1985.5 72 1349.5 73 1047.0 74 782.0 75 545.0 76 396.0 77 267.5 78 164.5 79 91.5 80 67.0 81 45.0 82 28.0 83 23.0 84 14.5 85 10.0 86 11.5 87 9.5 88 6.0 89 5.5 90 4.5 91 2.5 92 3.0 93 4.0 94 2.5 95 1.5 96 1.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.49223929892647555 2 0.051088201090329764 3 0.05848254598498276 4 0.06744538828153183 5 0.08178593595601037 6 0.11095998763127762 7 0.07053756887384127 8 0.05104338687884702 9 0.049878217380295645 10-11 0.07672193005846013 12-13 0.11013092471884685 14-15 0.05642109225677647 16-17 0.06202286869211965 18-19 0.060588813924671796 20-21 0.0780663564029425 22-23 0.06733335275282497 24-25 0.0924293111831624 26-27 0.06961887753844499 28-29 0.07024627649920342 30-31 0.07427955553265052 32-33 0.07360734236040933 34-35 0.07618415952066719 36-37 0.07055997597958263 38-39 0.041453145621539504 40-41 0.045083096751641884 42-43 0.043537006455487165 44-45 0.020681758599287007 46-47 0.027829625330784896 48-49 0.027067783735578225 50-51 0.025476879227940764 52-53 0.025230401064785662 54-55 0.024737444738475466 56-57 0.024939108690147822 58-59 0.021264343348562698 60-61 0.02863628113747432 62-63 0.019673438840925234 64-65 0.021690078357648777 66-67 0.015393681644323049 68-69 0.018597897765339343 70-71 0.042909607494728726 72-73 0.03838337213497144 74-75 0.033117702285748854 76-77 0.02312413312509663 78-79 0.014564618731892258 80-81 0.016962179046219137 82-83 0.021264343348562698 84-85 0.017948091698839534 86-87 0.009097284930997318 88-89 0.04335774960955618 90-91 0.0545837095859839 92-93 0.034551757053196706 94-95 0.056062578564914506 96-97 0.04510550385738325 98-99 0.0351343418024724 100-101 0.16859106359808823 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2231435.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.923413487705886 #Duplication Level Percentage of deduplicated Percentage of total 1 80.19974718756221 47.25642865142205 2 11.778845185793099 13.880995305803213 3 3.534742328059163 6.248372513061786 4 1.50324893999776 3.5430623546577436 5 0.7783335120230103 2.2931033680135067 6 0.46234302020653867 1.634569737167078 7 0.30404912423636615 1.254092858756798 8 0.2331686465010588 1.099127405612049 9 0.1654342823910957 0.877315736373519 >10 0.9354569604148568 10.064059598050532 >50 0.06208583039599777 2.516749585053331 >100 0.03652548090197332 4.0765662024463545 >500 0.003124045091095053 1.326269633025051 >1k 0.0026668677606907294 2.832533001960515 >5k 1.5239244346804167E-4 0.6209312466058097 >10k+ 7.619622173402083E-5 0.4758228019904823 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10598 0.47494101329413585 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8649 0.387598115114265 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5181 0.23218242969210395 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3563 0.1596730355130219 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3108 0.13928256928837274 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3065 0.13735555819461467 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2870 0.12861678695547932 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2814 0.12610719111244556 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2525 0.11315588399393216 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2447 0.109660375498278 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2374 0.1063889380600376 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2311 0.10356564273662464 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2242 0.10047346214431521 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.481421148274541E-5 2 0.0 0.0 0.0 0.0 4.481421148274541E-5 3 0.0 0.0 0.0 0.0 4.481421148274541E-5 4 0.0 0.0 0.0 0.0 4.481421148274541E-5 5 0.0 0.0 0.0 0.0 4.481421148274541E-5 6 0.0 0.0 0.0 0.0 4.481421148274541E-5 7 0.0 0.0 0.0 0.0 4.481421148274541E-5 8 0.0 0.0 0.0 0.0 4.481421148274541E-5 9 0.0 0.0 0.0 0.0 4.481421148274541E-5 10-11 8.962842296549081E-5 0.0 0.0 0.0 4.481421148274541E-5 12-13 8.962842296549081E-5 0.0 0.0 0.0 2.2407105741372704E-4 14-15 8.962842296549081E-5 0.0 0.0 0.0 2.4647816315509974E-4 16-17 8.962842296549081E-5 0.0 0.0 4.481421148274541E-5 3.1369948037921787E-4 18-19 8.962842296549081E-5 0.0 0.0 4.481421148274541E-5 3.1369948037921787E-4 20-21 8.962842296549081E-5 0.0 0.0 6.722131722411811E-5 3.1369948037921787E-4 22-23 8.962842296549081E-5 0.0 0.0 2.0166395167235435E-4 3.5851369186196326E-4 24-25 1.1203552870686352E-4 0.0 0.0 3.585136918619633E-4 3.5851369186196326E-4 26-27 1.3444263444823621E-4 0.0 0.0 9.186913353962808E-4 3.5851369186196326E-4 28-29 1.3444263444823621E-4 0.0 0.0 0.002868109534895706 3.5851369186196326E-4 30-31 1.3444263444823621E-4 0.0 0.0 0.009433391517117909 3.80920797603336E-4 32-33 1.3444263444823621E-4 0.0 0.0 0.022989690490648397 4.481421148274541E-4 34-35 1.568497401896089E-4 0.0 0.0 0.04665159415353797 4.481421148274541E-4 36-37 1.7925684593098163E-4 0.0 0.0 0.08507978049999215 4.481421148274541E-4 38-39 1.7925684593098163E-4 0.0 0.0 0.14206105040030295 4.705492205688268E-4 40-41 1.7925684593098163E-4 0.0 0.0 0.20238097905607827 4.929563263101995E-4 42-43 1.7925684593098163E-4 0.0 0.0 0.2626112792888881 4.929563263101995E-4 44-45 1.7925684593098163E-4 0.0 0.0 0.3365771353411594 4.929563263101995E-4 46-47 1.7925684593098163E-4 0.0 0.0 0.4249955745966161 4.929563263101995E-4 48-49 1.7925684593098163E-4 0.0 0.0 0.5152289894171239 5.377705377929449E-4 50-51 1.7925684593098163E-4 0.0 0.0 0.6137306262561983 5.377705377929449E-4 52-53 1.7925684593098163E-4 0.0 0.0 0.7069890003517916 6.722131722411811E-4 54-55 1.7925684593098163E-4 0.0 0.0 0.8082467111970548 6.722131722411811E-4 56-57 1.7925684593098163E-4 0.0 0.0 0.9242482976201413 6.946202779825538E-4 58-59 1.7925684593098163E-4 0.0 0.0 1.046098138641726 7.170273837239265E-4 60-61 1.7925684593098163E-4 0.0 0.0 1.1780087701411872 7.170273837239265E-4 62-63 1.7925684593098163E-4 0.0 0.0 1.3089110818822864 7.618415952066719E-4 64-65 1.7925684593098163E-4 0.0 0.0 1.4403287570554375 7.618415952066719E-4 66-67 1.7925684593098163E-4 0.0 0.0 1.5972457185622706 7.618415952066719E-4 68-69 2.2407105741372704E-4 0.0 0.0 1.7719539220277534 7.618415952066719E-4 70-71 2.2407105741372704E-4 0.0 0.0 1.948140994472167 7.618415952066719E-4 72-73 2.2407105741372704E-4 0.0 0.0 2.1242608455993564 7.618415952066719E-4 74-75 2.2407105741372704E-4 0.0 0.0 2.3023077078203036 7.618415952066719E-4 76-77 2.2407105741372704E-4 0.0 0.0 2.496913421184126 7.618415952066719E-4 78-79 2.2407105741372704E-4 0.0 0.0 2.717242491939044 7.618415952066719E-4 80-81 2.2407105741372704E-4 0.0 0.0 2.9488871510933548 8.066558066894174E-4 82-83 2.2407105741372704E-4 0.0 0.0 3.182705299504579 8.514700181721628E-4 84-85 2.2407105741372704E-4 0.0 0.0 3.425934432327179 8.514700181721628E-4 86-87 2.2407105741372704E-4 0.0 0.0 3.679358798262105 8.962842296549082E-4 88-89 2.2407105741372704E-4 0.0 0.0 3.943695424693079 9.410984411376536E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4595 0.0 57.03541 1 GTATCAA 8290 0.0 43.533646 1 TATCAAC 10380 0.0 34.79218 2 ATCAACG 10415 0.0 34.53838 3 TCAACGC 10475 0.0 34.43128 4 CAACGCA 10745 0.0 33.742985 5 TGGTATC 1860 0.0 32.95658 2 GTGGTAT 1870 0.0 32.93107 1 AACGCAG 11210 0.0 32.428078 6 ACGCAGA 12675 0.0 28.435411 7 CGCAGAG 12770 0.0 27.999836 8 GCAGAGT 13855 0.0 25.256918 9 TAAGGTG 1495 0.0 24.156712 5 GAGTACT 9050 0.0 23.742996 12-13 TAAGGTA 875 0.0 22.80902 4 GTGTAAG 1125 0.0 22.489565 1 CAGAGTA 13295 0.0 22.404335 10-11 GTACTTT 9570 0.0 22.139683 14-15 TACATGG 7605 0.0 21.806782 2 GTACATG 7760 0.0 21.469477 1 >>END_MODULE