FastQCFastQC Report
Thu 26 May 2016
SRR938205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938205_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231435
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101770.4560742302599No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88000.3943650610481596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52620.23581238082220632No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34810.15599827017143678No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC31790.14246437830364767No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA30970.13878961296206252No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30430.1363696455419943No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA29910.13403930654489152No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG25490.11423142506951806No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA24710.1107359165738639No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA24530.10992926076717449No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24010.10759892177007173No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA23310.10446192696627954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46450.053.0829121
GTATCAA81000.041.6434251
TATCAAC101900.033.1022342
ATCAACG102050.032.9065363
TCAACGC102750.032.63544
CAACGCA104850.031.936465
AACGCAG109100.030.779446
GTGGTAT21850.028.4834771
TGGTATC21400.027.972412
ACGCAGA124750.026.8419847
CGCAGAG126150.026.431148
TAAGGTG15250.024.6049965
GCAGAGT140100.023.5959349
TAGGACG4050.023.4553034
GTAAGGT15050.022.7228094
GTACATG79650.021.7710931
GAGTACT89700.021.7628312-13
GGTAAGG15950.021.4411263
TACATGG78550.021.4106662
CAGAGTA132050.021.31159610-11
Produced by FastQC (version 0.11.5)