##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938205_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2231435 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52161680712187 34.0 31.0 34.0 31.0 34.0 2 32.74286098407527 34.0 31.0 34.0 31.0 34.0 3 32.795482727482536 34.0 31.0 34.0 31.0 34.0 4 36.2497029938134 37.0 35.0 37.0 35.0 37.0 5 36.21504144194207 37.0 37.0 37.0 35.0 37.0 6 36.2523537544226 37.0 37.0 37.0 35.0 37.0 7 36.220369403545256 37.0 37.0 37.0 35.0 37.0 8 36.20889024327395 37.0 37.0 37.0 35.0 37.0 9 37.92030643957812 39.0 38.0 39.0 35.0 39.0 10-11 37.910779610430055 39.0 38.0 39.0 35.0 39.0 12-13 37.8624486037012 39.0 38.0 39.0 35.0 39.0 14-15 39.19242034834087 40.5 39.0 41.0 36.0 41.0 16-17 39.14846500122118 40.0 39.0 41.0 36.0 41.0 18-19 39.18090376820297 40.0 39.0 41.0 36.0 41.0 20-21 39.10813489973941 40.0 39.0 41.0 36.0 41.0 22-23 38.974624849032125 40.0 38.5 41.0 35.0 41.0 24-25 38.939902573904234 40.0 38.0 41.0 35.0 41.0 26-27 38.90198863063455 40.0 38.0 41.0 35.0 41.0 28-29 38.848601684566205 40.0 38.0 41.0 35.0 41.0 30-31 38.643389343628655 40.0 38.0 41.0 35.0 41.0 32-33 38.45833152209228 40.0 38.0 41.0 34.0 41.0 34-35 38.26842032145234 40.0 38.0 41.0 34.0 41.0 36-37 38.13546932803331 40.0 38.0 41.0 34.0 41.0 38-39 38.10366647471246 40.0 38.0 41.0 34.0 41.0 40-41 38.030189541707465 40.0 38.0 41.0 33.0 41.0 42-43 37.869520286273186 40.0 38.0 41.0 33.0 41.0 44-45 37.661743676154586 40.0 37.0 41.0 33.0 41.0 46-47 37.340419281762635 40.0 37.0 41.0 32.5 41.0 48-49 36.99074855418151 40.0 37.0 41.0 31.0 41.0 50-51 36.688256659952 40.0 36.0 41.0 31.0 41.0 52-53 36.27292213306684 39.0 35.5 41.0 30.0 41.0 54-55 36.049569671534236 39.0 35.0 41.0 29.5 41.0 56-57 35.80444646606332 39.0 35.0 40.5 29.0 41.0 58-59 35.46649353442964 38.5 35.0 40.0 28.0 41.0 60-61 35.1095297420718 38.0 35.0 40.0 28.0 41.0 62-63 34.74063707883044 37.5 34.0 40.0 26.5 41.0 64-65 34.35419449815926 37.0 34.0 40.0 26.0 41.0 66-67 33.94179037256295 36.0 34.0 39.0 26.0 41.0 68-69 33.48561015669289 36.0 33.0 39.0 25.5 40.5 70-71 32.97050754335215 35.0 33.0 38.5 23.5 40.0 72-73 32.56135289623045 35.0 32.0 37.5 23.0 40.0 74-75 32.12062125941378 35.0 32.0 37.0 22.0 39.0 76-77 30.83674070721307 34.0 30.5 35.5 20.5 38.5 78-79 31.541288677465396 35.0 31.5 36.0 22.0 38.5 80-81 31.38277184860863 35.0 32.0 36.0 20.5 37.5 82-83 31.01343866166839 35.0 31.0 35.0 19.5 37.0 84-85 30.638500561298 34.0 31.0 35.0 18.0 36.5 86-87 30.223758478288634 34.0 31.0 35.0 13.0 36.0 88-89 29.76676533262228 34.0 30.0 35.0 7.5 36.0 90-91 29.433342893698452 34.0 30.0 35.0 3.5 35.0 92-93 29.040695337305365 34.0 29.0 35.0 2.0 35.0 94-95 28.633404737310293 34.0 29.0 35.0 2.0 35.0 96-97 28.07739683208339 34.0 29.0 35.0 2.0 35.0 98-99 27.407738069896723 34.0 27.5 35.0 2.0 35.0 100-101 25.008698214377745 31.0 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22640294804768502 1101 2 0.2289976512549714 1101 3 0.1938347680668855 1101 4 0.09542005522663288 1101 5 0.17523240976630206 1101 6 0.13049315803288408 1101 7 0.1706434139123303 1101 8 0.1850107608312186 1101 9 0.1135019734379128 1101 10-11 0.1816087181193069 1101 12-13 0.22044576346657152 1101 14-15 0.1733547018469821 1101 16-17 0.22570422026835502 1101 18-19 0.24077038565179976 1101 20-21 0.21939765126374766 1101 22-23 0.23930375387499936 1101 24-25 0.20850085910743843 1101 26-27 0.25497785092809266 1101 28-29 0.20168268904217967 1101 30-31 0.22921084065521313 1101 32-33 0.24618865808791668 1101 34-35 0.20913263341339672 1101 36-37 0.23520338016996334 1101 38-39 0.2876593767174924 1101 40-41 0.35357448093756716 1101 42-43 0.4977581396029578 1101 44-45 0.5619349865593151 1101 46-47 0.6368114785369627 1101 48-49 0.594272779560562 1101 50-51 0.5007859601120899 1101 52-53 0.3913942955661227 1101 54-55 0.5630402698980603 1101 56-57 0.5646688717707207 1101 58-59 0.4341425374984027 1101 60-61 0.4722592443977902 1101 62-63 0.4552478978587189 1101 64-65 0.6057605732350453 1101 66-67 0.5445648661099014 1101 68-69 0.5737474962871261 1101 70-71 0.5782407640459795 1101 72-73 0.5922890529261053 1101 74-75 0.5358320457855683 1101 76-77 0.5780995604339729 1101 78-79 0.6039599110835212 1101 80-81 0.728007478704825 1101 82-83 0.7800828785459615 1101 84-85 0.7592393483403015 1101 86-87 0.7668149528197361 1101 88-89 0.817532083534843 1101 90-91 0.8017644977552258 1101 92-93 0.9876093370087808 1101 94-95 0.6563837901340186 1101 96-97 0.5998182319797287 1101 98-99 0.6379927450019522 1101 100-101 0.5595925832282944 1102 1 0.06629547677270153 1102 2 0.06599766972435361 1102 3 0.0943743305067315 1102 4 -0.01682427210825921 1102 5 0.024619226322258214 1102 6 -0.004001899355586147 1102 7 0.0385686149720641 1102 8 0.03879893345015262 1102 9 0.029234910295201644 1102 10-11 0.05304707758681815 1102 12-13 0.07998855907414537 1102 14-15 -0.014635249783765403 1102 16-17 0.059085137909278274 1102 18-19 0.1034951734078291 1102 20-21 0.1483632581983798 1102 22-23 0.12296153266516541 1102 24-25 0.019942845756126815 1102 26-27 0.029100657945640762 1102 28-29 -0.014496539881690751 1102 30-31 -0.011100173457450069 1102 32-33 0.019297316533659625 1102 34-35 0.10451782774207174 1102 36-37 0.11407925669600161 1102 38-39 0.003444720442935534 1102 40-41 0.12420439456243315 1102 42-43 -0.05309383754426733 1102 44-45 0.03102801683062495 1102 46-47 -0.01379093110158891 1102 48-49 -0.020568932715214316 1102 50-51 0.01634692651219183 1102 52-53 0.027890587324883143 1102 54-55 0.0327322724509429 1102 56-57 0.20091326169628587 1102 58-59 0.13463420780763613 1102 60-61 0.18132942907107719 1102 62-63 0.08727921679824391 1102 64-65 0.13791153271937873 1102 66-67 0.05649146879041922 1102 68-69 0.10802051067477692 1102 70-71 0.1246876656021314 1102 72-73 0.07383544801079012 1102 74-75 0.1844348884284912 1102 76-77 0.11058348181186872 1102 78-79 0.0801499292040404 1102 80-81 0.1850774550845351 1102 82-83 0.2953048548517998 1102 84-85 0.1936712507070517 1102 86-87 0.10406559052641384 1102 88-89 0.11950966683687625 1102 90-91 0.2380029364358549 1102 92-93 0.2997318690377675 1102 94-95 0.16667136352415568 1102 96-97 0.1318923639701346 1102 98-99 0.1714241199652662 1102 100-101 0.3351964037223283 1103 1 0.1394130083239702 1103 2 0.12711930614719336 1103 3 0.13522100960642547 1103 4 0.07720910957572613 1103 5 0.10238335408638477 1103 6 0.030243189106371915 1103 7 0.1203204379889442 1103 8 0.10846728745585921 1103 9 0.12178446971223167 1103 10-11 0.04720515954610249 1103 12-13 0.1178247934465233 1103 14-15 0.28144699072618806 1103 16-17 0.2721470908808712 1103 18-19 0.2631603698200209 1103 20-21 0.2128895451644297 1103 22-23 0.24230425983922288 1103 24-25 0.21385165050831034 1103 26-27 0.1059024597474405 1103 28-29 0.17841046898917767 1103 30-31 0.192832263908727 1103 32-33 0.2509767369178988 1103 34-35 0.18443442286950784 1103 36-37 0.17643494480469712 1103 38-39 0.11687473040909424 1103 40-41 0.2712704228031555 1103 42-43 0.21661139616699643 1103 44-45 0.13584581381710592 1103 46-47 0.17415791588339857 1103 48-49 0.31791411495458277 1103 50-51 0.40436336573344533 1103 52-53 0.4367470204282995 1103 54-55 0.5350987407812653 1103 56-57 0.4944913825334467 1103 58-59 0.44564964547970476 1103 60-61 0.4376785355707895 1103 62-63 0.5612920987930536 1103 64-65 0.60408963579507 1103 66-67 0.5975604128714096 1103 68-69 0.537534142598048 1103 70-71 0.46810013612303436 1103 72-73 0.4886281569300053 1103 74-75 0.5299607264242141 1103 76-77 0.6096997256570269 1103 78-79 0.5988844495951895 1103 80-81 0.6999092638922519 1103 82-83 0.7899548306764714 1103 84-85 0.7596997381210784 1103 86-87 0.9034545468430828 1103 88-89 0.7132508477768553 1103 90-91 0.770820077234923 1103 92-93 0.7029459538783556 1103 94-95 0.33996317943645593 1103 96-97 0.34073855956331656 1103 98-99 0.5636709944410612 1103 100-101 0.6053380267253985 1104 1 0.03807838304567923 1104 2 0.03439803173577616 1104 3 0.026408099385065498 1104 4 0.026765580258960142 1104 5 0.05067556435042775 1104 6 -0.01634988283881711 1104 7 -0.007131085237226387 1104 8 0.041604522001541966 1104 9 0.06270682612754541 1104 10-11 0.07561410626490783 1104 12-13 0.0440299358488474 1104 14-15 0.04771626896729941 1104 16-17 -0.01407581576457062 1104 18-19 0.09153182706471341 1104 20-21 0.14107989941111754 1104 22-23 0.028366250126559578 1104 24-25 0.09409296736934891 1104 26-27 0.1977626297766193 1104 28-29 0.11037053404783137 1104 30-31 0.08544799103149359 1104 32-33 0.10680721528358816 1104 34-35 0.15069620321579436 1104 36-37 0.1536834481095326 1104 38-39 0.10668797515192097 1104 40-41 0.21989773128961332 1104 42-43 0.16877886453164592 1104 44-45 0.15074067026124993 1104 46-47 0.25066124379517873 1104 48-49 0.25009046544079894 1104 50-51 0.4037981398161037 1104 52-53 0.35119719403340355 1104 54-55 0.1383505200893822 1104 56-57 0.2028244274862061 1104 58-59 0.23002633971555042 1104 60-61 0.27368688707209543 1104 62-63 0.27776439695549016 1104 64-65 0.249995785222346 1104 66-67 0.17828851613472807 1104 68-69 0.21531730347980016 1104 70-71 0.24926484756943523 1104 72-73 0.14888989113539708 1104 74-75 0.1902709085653811 1104 76-77 0.1442696348748278 1104 78-79 0.21016647425749113 1104 80-81 0.1595584777668151 1104 82-83 0.25508269862124067 1104 84-85 0.28824249342439856 1104 86-87 0.21025513744648805 1104 88-89 0.07429005599916039 1104 90-91 0.08599985368230634 1104 92-93 0.24409382389946543 1104 94-95 0.1719070951831867 1104 96-97 0.35514028523264685 1104 98-99 0.2826337472066278 1104 100-101 0.25853270668064 1105 1 0.04560034946364766 1105 2 0.044311743085430066 1105 3 0.06088882242811877 1105 4 0.06432852792528365 1105 5 0.041074060639097354 1105 6 0.04706300483676529 1105 7 -0.02820073204834017 1105 8 0.05374204680234129 1105 9 -0.030879810959312692 1105 10-11 0.04485600644796506 1105 12-13 0.0717261631445325 1105 14-15 0.12899840877238233 1105 16-17 0.0873774948085071 1105 18-19 0.02170227325525076 1105 20-21 0.13254043444764108 1105 22-23 0.12876732505852573 1105 24-25 0.1601906276234999 1105 26-27 0.058511310086657886 1105 28-29 0.015809659951692367 1105 30-31 0.1471462940284738 1105 32-33 0.10410944293303714 1105 34-35 0.174295690896912 1105 36-37 0.2454919934350528 1105 38-39 0.2018701744149709 1105 40-41 0.0377625080877948 1105 42-43 0.12237916203462618 1105 44-45 0.11540228193462099 1105 46-47 0.08483689841894204 1105 48-49 0.32647414198941505 1105 50-51 0.2505670571482099 1105 52-53 0.21963566568643955 1105 54-55 0.20863538558436545 1105 56-57 0.12386232075594705 1105 58-59 0.12626152050827955 1105 60-61 0.16911522955854963 1105 62-63 0.14355048890437416 1105 64-65 0.21166808867538833 1105 66-67 0.2566771411211377 1105 68-69 0.21755624781307148 1105 70-71 0.36447340866516953 1105 72-73 0.3135800596849023 1105 74-75 0.2922699964502371 1105 76-77 0.30143346823053463 1105 78-79 0.4209412606669787 1105 80-81 0.44191597575824204 1105 82-83 0.5805530879111345 1105 84-85 0.5678189992135607 1105 86-87 0.574176401450547 1105 88-89 0.49577835940105075 1105 90-91 0.33588413440066134 1105 92-93 0.4699973613292201 1105 94-95 0.32667417549833644 1105 96-97 0.4750124612452957 1105 98-99 0.6287383975021044 1105 100-101 0.6823367809370424 1106 1 -0.07229025629409591 1106 2 0.009881726129343349 1106 3 -0.025885633114569373 1106 4 -0.07462813329290441 1106 5 -0.032555661570121686 1106 6 -0.006067561105723485 1106 7 0.03480242915254905 1106 8 -0.07695376343604465 1106 9 -0.07058229574870722 1106 10-11 0.014774930703318034 1106 12-13 -0.06662502530822678 1106 14-15 -0.20091107055564095 1106 16-17 -0.15095975016318874 1106 18-19 -0.1323360438378316 1106 20-21 0.03257972045998514 1106 22-23 0.02299034747597517 1106 24-25 0.03848337558846282 1106 26-27 -0.09627303413208921 1106 28-29 0.02342870369350436 1106 30-31 0.034656395418956265 1106 32-33 0.002400806727614224 1106 34-35 0.023633528212108956 1106 36-37 0.017679532237622197 1106 38-39 -0.05467857993345859 1106 40-41 -0.05918814420250129 1106 42-43 -0.25749642547445717 1106 44-45 0.02389905254297986 1106 46-47 -0.008928431776944024 1106 48-49 -0.02607008530764432 1106 50-51 -0.09070279704572215 1106 52-53 -0.19133001706578057 1106 54-55 -0.33001512971377167 1106 56-57 -0.03828591167882678 1106 58-59 0.007891136869176307 1106 60-61 0.04959059030795032 1106 62-63 -0.09226339310324505 1106 64-65 -0.20237900161526312 1106 66-67 -0.025071554603606216 1106 68-69 -0.07274560310770539 1106 70-71 -0.22410301280657308 1106 72-73 -0.032520243814808225 1106 74-75 -0.24222556504459192 1106 76-77 -0.13991740064171054 1106 78-79 -0.13691451692126932 1106 80-81 -0.5213779630003614 1106 82-83 -0.5688799111179108 1106 84-85 -0.3340211331782683 1106 86-87 -0.06459064878059095 1106 88-89 -0.06183702036033978 1106 90-91 -0.4520030516420164 1106 92-93 -0.17282337435733908 1106 94-95 -0.03754257744149925 1106 96-97 -0.0017714392247079047 1106 98-99 -0.10813132153166194 1106 100-101 -8.0117403263813E-4 1107 1 0.0554766295235396 1107 2 0.11468053237911846 1107 3 -0.011295868632508643 1107 4 0.0352110162181134 1107 5 0.0624914749195824 1107 6 0.025997251325691195 1107 7 0.10808123602164699 1107 8 0.059584709268143854 1107 9 0.045685260682510886 1107 10-11 0.09486941657591785 1107 12-13 0.11412391361271546 1107 14-15 0.24171459933216255 1107 16-17 0.1124003625540837 1107 18-19 0.14865249670187808 1107 20-21 0.13958158424317446 1107 22-23 0.08613359943895205 1107 24-25 0.1781509912426955 1107 26-27 0.17068100609698433 1107 28-29 0.14447880907564326 1107 30-31 0.06697106705055234 1107 32-33 0.10615759753682852 1107 34-35 0.17227623729743158 1107 36-37 0.06468693124399749 1107 38-39 0.007944051387781315 1107 40-41 0.10820329478134028 1107 42-43 0.08611784301483283 1107 44-45 0.14626193480723515 1107 46-47 0.30146404651279823 1107 48-49 0.23120737839890637 1107 50-51 0.19622439928631508 1107 52-53 0.28203762432917046 1107 54-55 0.25331746171902125 1107 56-57 0.17304271606967347 1107 58-59 0.2943569355767224 1107 60-61 0.33184874847211887 1107 62-63 0.27394571446782123 1107 64-65 0.2993125316221992 1107 66-67 0.38265975869583 1107 68-69 0.5117259669409293 1107 70-71 0.32354567857763783 1107 72-73 0.23821729564300398 1107 74-75 0.40131167481443697 1107 76-77 0.36319567854875245 1107 78-79 0.5670018470177212 1107 80-81 0.5417323872863662 1107 82-83 0.4838067042866534 1107 84-85 0.6794848113804761 1107 86-87 0.6602014416564401 1107 88-89 0.8269859899107885 1107 90-91 0.7946162043543161 1107 92-93 0.9498981361779677 1107 94-95 0.8939437461058439 1107 96-97 0.903240163454651 1107 98-99 0.9700638666949573 1107 100-101 1.2274329155876558 1108 1 0.07620981443023567 1108 2 0.023276914581018104 1108 3 -0.015624488297895311 1108 4 -0.010691043541818601 1108 5 -0.019498420736567823 1108 6 0.018112132218277566 1108 7 -0.025278846485022655 1108 8 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#Quality Count 2 9.0 3 7.0 4 11.0 5 32.0 6 63.0 7 180.0 8 396.0 9 891.0 10 1536.0 11 2533.0 12 3793.0 13 5494.0 14 7597.0 15 10560.0 16 13644.0 17 19545.0 18 19504.0 19 13925.0 20 11647.0 21 11316.0 22 11555.0 23 12316.0 24 14085.0 25 16364.0 26 19419.0 27 23602.0 28 28994.0 29 35750.0 30 43413.0 31 53821.0 32 67109.0 33 87943.0 34 122500.0 35 187649.0 36 315071.0 37 529003.0 38 478937.0 39 61213.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09218059230943 18.841328562113617 15.129098539728918 22.937392305848032 2 15.85636092101984 19.773725153422628 39.75461343593182 24.615300489625707 3 19.577983468733514 23.435443311416236 29.70200606197231 27.28456715787794 4 13.126172171719094 16.835982226683726 36.50883848285969 33.52900711873749 5 13.71928826069323 37.124047978094815 34.18795528437978 14.968708476832173 6 32.664749512981125 36.02877612726889 16.792079349906537 14.51439500984345 7 29.06613905401681 32.62039898092483 19.84749723832422 18.465964726734143 8 26.80582711795894 34.57403305236241 19.26353023107614 19.35660959860251 9 26.071680652497843 16.800699104832116 20.05772096306172 37.06989927960832 10-11 25.120583839547194 26.485848792369037 26.663537140898118 21.730030227185644 12-13 26.266483227160997 23.656660400146095 27.2260227163238 22.850833656369108 14-15 22.70807798569082 25.229572001873237 25.800549870374894 26.26180014206105 16-17 22.111172282687974 28.371668641858793 26.937251182370396 22.57990789308284 18-19 22.11865906916401 26.50908496102284 29.47816091438917 21.894095055423975 20-21 22.44017862944697 25.627432571417046 30.076251380837892 21.85613741829809 22-23 22.29524946951177 25.727726776715432 29.87996513454347 22.09705861922933 24-25 22.235052187683397 25.8593394094038 29.84162076150837 22.06398764140443 26-27 21.554938683594436 26.332436712477854 29.75272179936162 22.359902804566087 28-29 21.794818937060203 26.731218579621896 29.467777223077178 22.006185260240724 30-31 21.88519943444465 26.18097771165192 29.455328073638714 22.478494780264718 32-33 21.480442452197078 26.21521425186344 29.53674273531181 22.76760056062767 34-35 22.434059722980464 26.37516954022216 29.282565841668035 21.908204895129344 36-37 22.498616311613347 25.80111636203114 29.21892751265487 22.481339813700643 38-39 21.925358279496443 26.330447465301564 29.130324383937655 22.613869871264335 40-41 22.614492953693652 25.967864253385553 29.24564496942761 22.171997823493186 42-43 22.078479389009175 26.08029719517349 29.09758203993168 22.743641375885655 44-45 22.120404154117423 26.408609214984562 29.278608966259167 22.192377664638848 46-47 22.654199048683495 26.6481778085303 28.34860901106643 22.349014131719784 48-49 22.326305718069314 26.686638866917477 28.316285260381775 22.670770154631438 50-51 22.58427872646974 27.073968096762847 28.142518155357426 22.199235021409987 52-53 22.933493469449033 26.616683882792913 28.19656409440562 22.25325855335244 54-55 22.34371603923036 26.921062007183714 28.379831812264307 22.355390141321614 56-57 22.504554244241934 27.07520048757862 28.03124446824577 22.389000799933676 58-59 22.419160764261562 26.66938539549662 27.948472619637137 22.96298122060468 60-61 21.973461023959917 27.040805580265616 28.315321754834894 22.670411640939577 62-63 22.206158817084074 26.986849269640388 28.490410879097976 22.316581034177556 64-65 21.860394768388954 27.03735488598144 28.25845252046329 22.843797825166316 66-67 21.795727861219348 27.071682571977224 28.23985462269795 22.892734944105474 68-69 22.52533633708795 26.56698318275348 28.439527217866395 22.468153262292176 70-71 22.218482725241827 26.95162529941495 27.861533049360617 22.968358925982606 72-73 22.21102115902995 27.011878006753502 27.85933715299796 22.917763681218588 74-75 22.11827814836641 27.44657585813613 27.729196682852063 22.705949310645394 76-77 22.28169317053824 26.928568387607076 28.074086854423275 22.715651587431406 78-79 22.272439035867052 27.170296244345003 27.939576998657817 22.617687721130125 80-81 22.497406377510437 27.30646422593533 27.561815602964014 22.634313793590223 82-83 22.38613269039878 27.37706901612639 27.709388801376694 22.527409492098137 84-85 22.33010035063566 27.545581739697838 27.621521428929952 22.50279648073655 86-87 22.526688645133504 27.5739308464787 27.2344170870069 22.664963421380897 88-89 22.38183820972362 27.712361389457534 27.14487826651227 22.76092213430658 90-91 22.28736729361889 27.639137234867327 27.243337232178465 22.83015823933531 92-93 22.402408323819618 27.24347979040323 27.52576545731376 22.828346428463394 94-95 22.1292128159682 27.22308738547168 27.62659454566232 23.0211052528978 96-97 22.461913522016104 26.797957368240617 27.652810859379727 23.087318250363555 98-99 22.14700405792685 27.152437781069132 27.298711367348815 23.401846793655203 100-101 22.25935328611409 26.92119644981817 27.60869126817496 23.21075899589278 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 649.0 1 449.5 2 386.5 3 548.0 4 458.0 5 397.0 6 838.5 7 1907.0 8 3110.0 9 3666.5 10 2963.5 11 2103.5 12 2038.5 13 2103.5 14 2188.5 15 2439.0 16 2682.5 17 2875.0 18 3183.5 19 3542.0 20 4008.5 21 4683.5 22 5638.5 23 6919.5 24 8470.5 25 10608.5 26 13724.0 27 17032.0 28 19862.5 29 22977.5 30 26502.0 31 31226.0 32 39083.0 33 42931.0 34 42765.0 35 45170.0 36 49184.5 37 53522.0 38 56426.0 39 58743.5 40 61999.5 41 66057.0 42 68181.0 43 70299.5 44 73366.0 45 77089.5 46 79029.5 47 80933.0 48 81451.0 49 79610.0 50 79792.5 51 78241.0 52 76804.5 53 78213.0 54 80605.0 55 76378.5 56 69425.0 57 64367.0 58 59735.5 59 52915.0 60 41585.0 61 33822.0 62 28782.5 63 20793.5 64 14010.5 65 10648.0 66 7881.5 67 5913.5 68 4525.5 69 3318.5 70 2430.0 71 1623.0 72 1113.0 73 913.0 74 664.5 75 431.0 76 329.5 77 208.0 78 112.5 79 68.5 80 38.0 81 20.0 82 10.5 83 6.5 84 4.0 85 2.5 86 1.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015371274538581677 3 0.00663250329944632 4 0.0 5 0.0 6 8.962842296549081E-5 7 0.0 8 2.6888526889647243E-4 9 4.4814211482745414E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.912923746378451E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0050864130032916045 26-27 0.02088342255095936 28-29 4.481421148274541E-5 30-31 0.0 32-33 3.3610658612059056E-4 34-35 0.017970498804580907 36-37 0.003585136918619633 38-39 0.017499949584012083 40-41 0.015102389269685204 42-43 0.007797672797997701 44-45 0.0026216313717406063 46-47 4.481421148274541E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.4647816315509974E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0026440384774819793 86-87 3.8092079760333595E-4 88-89 1.3444263444823621E-4 90-91 2.2407105741372707E-4 92-93 3.3610658612059056E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2231435.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.253248119496114 #Duplication Level Percentage of deduplicated Percentage of total 1 78.92702663726804 45.18828639397586 2 12.555040521860796 14.376337002968484 3 3.6834055275077255 6.326607917733698 4 1.638236468564406 3.751774360525001 5 0.8507198797470857 2.4353238177673897 6 0.5025360640941468 1.7263093179946312 7 0.343256024705266 1.3756765645673739 8 0.23113463716337587 1.0586566984419572 9 0.18346353836014898 0.9453495134352872 >10 0.9670302008833657 10.211713720490225 >50 0.0723457163468455 2.8371661612808485 >100 0.03923161633958294 4.283102279676151 >500 0.003443087559616186 1.3866158953331942 >1k 0.002895323629677098 3.0111293804633426 >5k 1.5650397998254583E-4 0.6300029958053195 >10k+ 7.825198999127291E-5 0.4559479795413694 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10177 0.4560742302599 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8800 0.3943650610481596 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5262 0.23581238082220632 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3481 0.15599827017143678 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3179 0.14246437830364767 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3097 0.13878961296206252 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3043 0.1363696455419943 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2991 0.13403930654489152 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2549 0.11423142506951806 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2471 0.1107359165738639 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2453 0.10992926076717449 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2401 0.10759892177007173 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2331 0.10446192696627954 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 8.962842296549081E-5 0.0 4 0.0 0.0 0.0 8.962842296549081E-5 0.0 5 0.0 0.0 0.0 8.962842296549081E-5 4.481421148274541E-5 6 0.0 0.0 0.0 8.962842296549081E-5 4.481421148274541E-5 7 0.0 0.0 0.0 8.962842296549081E-5 4.481421148274541E-5 8 0.0 0.0 0.0 8.962842296549081E-5 4.481421148274541E-5 9 0.0 0.0 0.0 8.962842296549081E-5 4.481421148274541E-5 10-11 8.962842296549081E-5 0.0 0.0 8.962842296549081E-5 4.481421148274541E-5 12-13 8.962842296549081E-5 0.0 0.0 8.962842296549081E-5 2.2407105741372704E-4 14-15 1.1203552870686352E-4 0.0 0.0 8.962842296549081E-5 2.4647816315509974E-4 16-17 1.568497401896089E-4 0.0 0.0 1.568497401896089E-4 2.6888526889647243E-4 18-19 1.7925684593098163E-4 0.0 0.0 1.7925684593098163E-4 2.6888526889647243E-4 20-21 1.7925684593098163E-4 0.0 0.0 2.4647816315509974E-4 3.3610658612059056E-4 22-23 1.7925684593098163E-4 0.0 0.0 3.80920797603336E-4 3.5851369186196326E-4 24-25 2.6888526889647243E-4 0.0 0.0 5.825847492756902E-4 3.5851369186196326E-4 26-27 3.80920797603336E-4 0.0 0.0 0.0011651694985513805 3.5851369186196326E-4 28-29 4.033279033447087E-4 0.0 0.0 0.0031594019095335514 4.929563263101995E-4 30-31 4.481421148274541E-4 0.0 0.0 0.009679869680273009 4.929563263101995E-4 32-33 4.481421148274541E-4 0.0 0.0 0.02346023971121722 4.929563263101995E-4 34-35 4.705492205688268E-4 0.0 0.0 0.04709973626836542 5.377705377929449E-4 36-37 4.929563263101995E-4 0.0 0.0 0.08552792261481962 5.377705377929449E-4 38-39 4.929563263101995E-4 0.0 0.0 0.1431814056873716 5.825847492756904E-4 40-41 4.929563263101995E-4 0.0 0.0 0.20385984803500884 5.825847492756904E-4 42-43 4.929563263101995E-4 0.0 0.0 0.26444866195968064 5.825847492756904E-4 44-45 5.153634320515722E-4 0.0 0.0 0.3392659880301241 6.04991855017063E-4 46-47 5.377705377929449E-4 0.0 0.0 0.4282446049291151 6.498060664998084E-4 48-49 5.377705377929449E-4 0.0 0.0 0.5190606044988987 7.170273837239265E-4 50-51 5.377705377929449E-4 0.0 0.0 0.6181672331929902 8.066558066894174E-4 52-53 5.825847492756904E-4 0.0 0.0 0.7120305991436005 8.514700181721628E-4 54-55 5.825847492756904E-4 0.0 0.0 0.8138484876323979 8.514700181721628E-4 56-57 5.825847492756904E-4 0.0 0.0 0.9311272790827427 8.514700181721628E-4 58-59 5.825847492756904E-4 0.0 0.0 1.0535372977478619 8.514700181721628E-4 60-61 5.825847492756904E-4 0.0 0.0 1.1867475413803226 8.514700181721628E-4 62-63 5.825847492756904E-4 0.0 0.0 1.3185013231395941 8.514700181721628E-4 64-65 5.825847492756904E-4 0.0 0.0 1.4507256541194344 8.514700181721628E-4 66-67 5.825847492756904E-4 0.0 0.0 1.6087405638075947 8.738771239135355E-4 68-69 5.825847492756904E-4 0.0 0.0 1.784367458608474 8.962842296549082E-4 70-71 5.825847492756904E-4 0.0 0.0 1.9614508152825425 8.962842296549082E-4 72-73 5.825847492756904E-4 0.0 0.0 2.1392960135518173 9.635055468790262E-4 74-75 5.825847492756904E-4 0.0 0.0 2.3186873021172474 0.0010307268641031443 76-77 5.825847492756904E-4 0.0 0.0 2.5148839199887068 0.0010307268641031443 78-79 5.825847492756904E-4 0.0 0.0 2.7391790484598477 0.0010307268641031443 80-81 5.825847492756904E-4 0.0 0.0 2.97411755215814 0.0010755410755858897 82-83 5.825847492756904E-4 0.0 0.0 3.212058608025777 0.001209983710034126 84-85 5.825847492756904E-4 0.0 0.0 3.4591193559301527 0.001209983710034126 86-87 5.825847492756904E-4 0.0 0.0 3.7161064516779563 0.0012996121329996169 88-89 5.825847492756904E-4 0.0 0.0 3.98600004033279 0.0013444263444823623 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4645 0.0 53.082912 1 GTATCAA 8100 0.0 41.643425 1 TATCAAC 10190 0.0 33.102234 2 ATCAACG 10205 0.0 32.906536 3 TCAACGC 10275 0.0 32.6354 4 CAACGCA 10485 0.0 31.93646 5 AACGCAG 10910 0.0 30.77944 6 GTGGTAT 2185 0.0 28.483477 1 TGGTATC 2140 0.0 27.97241 2 ACGCAGA 12475 0.0 26.841984 7 CGCAGAG 12615 0.0 26.43114 8 TAAGGTG 1525 0.0 24.604996 5 GCAGAGT 14010 0.0 23.595934 9 TAGGACG 405 0.0 23.455303 4 GTAAGGT 1505 0.0 22.722809 4 GTACATG 7965 0.0 21.771093 1 GAGTACT 8970 0.0 21.76283 12-13 GGTAAGG 1595 0.0 21.441126 3 TACATGG 7855 0.0 21.410666 2 CAGAGTA 13205 0.0 21.311596 10-11 >>END_MODULE