FastQCFastQC Report
Thu 26 May 2016
SRR938204_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938204_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212092
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99860.45142787912980115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84670.3827598490478696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49460.22358925397316207No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34540.1561417879545697No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31060.1404100733604208No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29830.13484972596076475No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA28200.12748113550430995No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28130.12716469296937016No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA24500.11075488722892177No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA24410.11034803254114205No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG23910.10808772872014365No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22570.10203011447986793No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA22550.10193970232702798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45950.055.3351481
GTATCAA81100.042.3898351
TGGTATC18200.036.325872
GTGGTAT18950.036.1825071
ATCAACG104150.032.9274753
TATCAAC104800.032.722512
TCAACGC105600.032.4753464
CAACGCA107900.031.8271835
AACGCAG111600.030.7677976
ACGCAGA126150.026.9472397
CGCAGAG125450.026.7919838
GCAGAGT135550.024.128779
TAAGGTA8150.023.3445154
GTGTAAG10100.022.6289921
GAGTACT90600.022.61237512-13
GTAAGGT16050.022.522734
TAAGGTG17950.021.993595
GGACCGT1954.1757448E-721.9498396
CAGAGTA129700.021.8418110-11
AGAGTAC124450.021.36916410-11
Produced by FastQC (version 0.11.5)